Rorug04G0386600

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014118
Physical Location & Seq
Reverse (-)
55610463 .. 55612585
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0386600.1

Sequence Viewer

Length: 786 bp
ATGCTCTTGATCCCATGTACTTCCGAAGAAAAGCTAGAACTGTACAGCATTGCCGAAAGAGAAGTCTACAGCAATATTGAGTTACCACTGCCTTGTAGTAATTATAGGAGGTGTTTTGGCTTCAACCACGACAGTGGTGGTTGGTTGACCATAGTAGATCTAGCAGAGCGTGAGTTGCCATATTTAGATATAACTCTCACGAACCCTTTCAGAAAAGAAGTGGCACCCATTCACCTTCCTCGTCTGCAATTCAAATATTTATCTCAAAAAGGACGTCGGCAGCTCCCGAAGGTTATATTATCCGGTGATCCCACATTGAATCCGGACAGTTTTGTGGTTTTAGCAATCAATAGTCAATTTCAAAATGGACACCAGGTGTCTTCCATTAAGGGACTAAATAGTGATTGGGTTCATTGGGACTTTGCATATAACGTTACTGATGCCATAGTTTATAGAAGCCAGATCTACTTGGTTACTGTTGATAGATGGCTCGCTTTGTTGGATGTTGATCAGGCAATAACAAACATATTCCTTCCGCCTCAACCACTTCTTCCGCCAGCTAAGGTGTATCTTGTAGAATCTACACGGGGAGACCTGTTGCTTGTTCAAAGATTAATGGAACTAAATAAGAATGTGAACGGTTTGCATCAGAAATTTTGGACCAATAGCTTCGTTATTCACAAGGTGGTGTTCATCAAAGAGCTGGACGGAGATATAGAATACTTAAAGTTTACAGAGGGGACGATGTCTGAAGGTGATCGAGATATATCGTTCACGGTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

261

Amino Acids

30.01

Weight (kDa)

5.54

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 14 - 227 1.3e-21 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35760
fragaria_vesca FvH4_3g00580
malus_domestica MD00G1058300.v1.1 MD10G1336500.v1.1
prunus_persica Prupe.4G007000_v2.0.a1
pyrus_communis pycom05g32590
rosa_chinensis RchiOBHm_Chr5g0000791
rosa_laevigata RLG00000030896
rosa_multiflora Rmu_sc0011992.1_g000001 Rmu_sc0040466.1_g000001
rosa_roxburghii Rroxscaffold_1G00075440
rosa_rugosa Rorug04G0386600
rosa_samantha Rh5AG006500 Rh5BG008400 Rh5CG007100 Rh5DG007100
rosa_wichuraiana Rw5G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 277
AccB1I GGYRCC 1 cut(s) 223
AccI GTMKAC 1 cut(s) 66
AccIII TCCGGA 1 cut(s) 322
AciI CCGC 2 cut(s) 536, 554
AclI AACGTT 1 cut(s) 432
AclWI GGATC 2 cut(s) 4, 302
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 1 cut(s) 771
AcyI GRCGYC 1 cut(s) 274
AdeI CACNNNGTG 2 cut(s) 376, 685
AfaI GTAC 2 cut(s) 19, 44
AgsI TTSAA 5 cut(s) 124, 253, 319, 362, 608
AjnI CCWGG 1 cut(s) 372
AleI CACNNNNGTG 1 cut(s) 132
AluBI AGCT 5 cut(s) 34, 283, 560, 669, 703
AluI AGCT 5 cut(s) 34, 283, 560, 669, 703
Alw26I GTCTC 1 cut(s) 585
AlwI GGATC 2 cut(s) 4, 302
Aor13HI TCCGGA 1 cut(s) 322
ApeKI GCWGC 1 cut(s) 280
ApoI RAATTY 1 cut(s) 653
ArsI GACNNNNNNTTYG 4 cut(s) 48, 80, 259, 291
AseI ATTAAT 1 cut(s) 614
Asp700I GAANNNNTTC 1 cut(s) 206
AspS9I GGNCC 1 cut(s) 660
AsuHPI GGTGA 3 cut(s) 224, 317, 767
AvaII GGWCC 1 cut(s) 660
BanI GGYRCC 1 cut(s) 223
BbsI GAAGAC 1 cut(s) 372
BbvI GCAGC 1 cut(s) 292
BccI CCATC 1 cut(s) 480
BciT130I CCWGG 1 cut(s) 374
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 1 cut(s) 585
BfaI CTAG 2 cut(s) 35, 161
BfmI CTRYAG 1 cut(s) 67
BglII AGATCT 2 cut(s) 157, 462
BisI GCNGC 1 cut(s) 281
BlsI GCNGC 1 cut(s) 282
Bme1390I CCNGG 1 cut(s) 374
Bme18I GGWCC 1 cut(s) 660
BmgT120I GGNCC 1 cut(s) 660
BmiI GGNNCC 1 cut(s) 225
BmrFI CCNGG 1 cut(s) 374
BmsI GCATC 2 cut(s) 430, 655
BpiI GAAGAC 1 cut(s) 372
Bpu10I CCTNAGC 1 cut(s) 561
BsaBI GATNNNNATC 1 cut(s) 507
BsaHI GRCGYC 1 cut(s) 274
BsaI GGTCTC 1 cut(s) 585
BsaWI WCCGGW 2 cut(s) 302, 322
Bse3DI GCAATG 1 cut(s) 48
Bse8I GATNNNNATC 1 cut(s) 507
BseAI TCCGGA 1 cut(s) 322
BseBI CCWGG 1 cut(s) 374
BseGI GGATG 1 cut(s) 508
BseJI GATNNNNATC 1 cut(s) 507
BseMI GCAATG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 292
Bsh1285I CGRYCG 1 cut(s) 780
BshNI GGYRCC 1 cut(s) 223
BsiEI CGRYCG 1 cut(s) 780
BsiSI CCGG 2 cut(s) 303, 323
BslFI GGGAC 3 cut(s) 405, 431, 754
BsmAI GTCTC 1 cut(s) 585
BsmFI GGGAC 3 cut(s) 405, 431, 754
Bso31I GGTCTC 1 cut(s) 585
Bsp13I TCCGGA 1 cut(s) 322
Bsp1407I TGTACA 1 cut(s) 42
Bsp143I GATC 6 cut(s) 9, 157, 307, 462, 508, 757
BspACI CCGC 2 cut(s) 536, 554
BspEI TCCGGA 1 cut(s) 322
BspLI GGNNCC 1 cut(s) 225
BspPI GGATC 2 cut(s) 4, 302
BspT107I GGYRCC 1 cut(s) 223
BspTNI GGTCTC 1 cut(s) 585
BsrDI GCAATG 1 cut(s) 48
BsrGI TGTACA 1 cut(s) 42
BssMI GATC 6 cut(s) 9, 157, 307, 462, 508, 757
BssNI GRCGYC 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 6 cut(s) 42, 134, 329, 478, 641, 778
BstACI GRCGYC 1 cut(s) 274
BstAUI TGTACA 1 cut(s) 42
BstC8I GCNNGC 2 cut(s) 492, 558
BstDEI CTNAG 1 cut(s) 561
BstF5I GGATG 1 cut(s) 508
BstKTI GATC 6 cut(s) 12, 160, 310, 465, 511, 760
BstMAI GTCTC 1 cut(s) 585
BstMBI GATC 6 cut(s) 9, 157, 307, 462, 508, 757
BstMCI CGRYCG 1 cut(s) 780
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 372
BstSFI CTRYAG 1 cut(s) 67
BstV1I GCAGC 1 cut(s) 292
BstV2I GAAGAC 1 cut(s) 372
BstX2I RGATCY 2 cut(s) 157, 462
BstXI CCANNNNNNTGG 1 cut(s) 134
BstYI RGATCY 2 cut(s) 157, 462
BtsCI GGATG 1 cut(s) 508
BtsI GCAGTG 1 cut(s) 86
BtsIMutI CAGTG 2 cut(s) 86, 139
Cac8I GCNNGC 2 cut(s) 492, 558
Cfr13I GGNCC 1 cut(s) 660
CsiI ACCWGGT 1 cut(s) 372
Csp6I GTAC 2 cut(s) 18, 43
CviAII CATG 1 cut(s) 15
CviJI RGCY 8 cut(s) 34, 120, 283, 459, 490, 560, 669, 703
CviKI_1 RGCY 8 cut(s) 34, 120, 283, 459, 490, 560, 669, 703
CviQI GTAC 2 cut(s) 18, 43
DdeI CTNAG 1 cut(s) 561
DpnI GATC 6 cut(s) 11, 159, 309, 464, 510, 759
DpnII GATC 6 cut(s) 9, 157, 307, 462, 508, 757
DraIII CACNNNGTG 2 cut(s) 376, 685
EciI GGCGGA 2 cut(s) 525, 543
Eco31I GGTCTC 1 cut(s) 585
Eco47I GGWCC 1 cut(s) 660
Eco57I CTGAAG 1 cut(s) 771
EcoRII CCWGG 1 cut(s) 372
FaeI CATG 1 cut(s) 18
FaqI GGGAC 3 cut(s) 405, 431, 754
FatI CATG 1 cut(s) 14
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 281
FokI GGATG 1 cut(s) 515
Fsp4HI GCNGC 1 cut(s) 281
FspBI CTAG 2 cut(s) 35, 161
GluI GCNGC 1 cut(s) 281
HapII CCGG 2 cut(s) 303, 323
Hin1I GRCGYC 1 cut(s) 274
Hin1II CATG 1 cut(s) 18
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HinfI GANTC 2 cut(s) 319, 578
HpaII CCGG 2 cut(s) 303, 323
HphI GGTGA 3 cut(s) 224, 317, 767
Hpy166II GTNNAC 5 cut(s) 67, 147, 637, 732, 774
Hpy188I TCNGA 4 cut(s) 25, 212, 651, 751
Hpy188III TCNNGA 5 cut(s) 7, 199, 286, 323, 761
Hpy8I GTNNAC 5 cut(s) 67, 147, 637, 732, 774
Hpy99I CGWCG 1 cut(s) 279
HpyAV CCTTC 4 cut(s) 245, 283, 542, 746
HpyCH4III ACNGT 6 cut(s) 42, 134, 329, 478, 641, 778
HpyCH4IV ACGT 2 cut(s) 274, 432
HpyCH4V TGCA 3 cut(s) 247, 425, 646
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 1 cut(s) 561
HpySE526I ACGT 2 cut(s) 274, 432
Hsp92I GRCGYC 1 cut(s) 274
Hsp92II CATG 1 cut(s) 18
Kpn2I TCCGGA 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 6 cut(s) 9, 157, 307, 462, 508, 757
LmnI GCTCC 1 cut(s) 288
LpnPI CCDG 9 cut(s) 316, 336, 359, 386, 473, 497, 570, 608, 689
Lsp1109I GCAGC 1 cut(s) 292
LweI GCATC 2 cut(s) 430, 655
MabI ACCWGGT 1 cut(s) 372
MaeI CTAG 2 cut(s) 35, 161
MaeII ACGT 2 cut(s) 274, 432
MaeIII GTNAC 3 cut(s) 81, 433, 472
MalI GATC 6 cut(s) 11, 159, 309, 464, 510, 759
MboI GATC 6 cut(s) 9, 157, 307, 462, 508, 757
MboII GAAGA 3 cut(s) 38, 372, 542
MflI RGATCY 2 cut(s) 157, 462
MluCI AATT 4 cut(s) 100, 248, 356, 653
MmeI TCCRAC 1 cut(s) 480
MnlI CCTC 4 cut(s) 102, 249, 549, 730
MroI TCCGGA 1 cut(s) 322
MroXI GAANNNNTTC 1 cut(s) 206
MseI TTAA 3 cut(s) 387, 614, 725
MslI CAYNNNNRTG 1 cut(s) 132
MspI CCGG 2 cut(s) 303, 323
MspR9I CCNGG 1 cut(s) 374
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 1 cut(s) 175
NdeII GATC 6 cut(s) 9, 157, 307, 462, 508, 757
NlaIII CATG 1 cut(s) 18
NlaIV GGNNCC 1 cut(s) 225
OliI CACNNNNGTG 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 2 cut(s) 319, 578
PflFI GACNNNGTC 1 cut(s) 745
PkrI GCNGC 1 cut(s) 282
PshBI ATTAAT 1 cut(s) 614
Psp1406I AACGTT 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 1 cut(s) 225
PspPI GGNCC 1 cut(s) 660
PsuI RGATCY 2 cut(s) 157, 462
PsyI GACNNNGTC 1 cut(s) 745
RsaI GTAC 2 cut(s) 19, 44
RsaNI GTAC 2 cut(s) 18, 43
RseI CAYNNNNRTG 1 cut(s) 132
SaqAI TTAA 3 cut(s) 387, 614, 725
SatI GCNGC 1 cut(s) 281
Sau3AI GATC 6 cut(s) 9, 157, 307, 462, 508, 757
Sau96I GGNCC 1 cut(s) 660
ScrFI CCNGG 1 cut(s) 374
SexAI ACCWGGT 1 cut(s) 372
SfaNI GCATC 2 cut(s) 430, 655
SfcI CTRYAG 1 cut(s) 67
SinI GGWCC 1 cut(s) 660
SmiMI CAYNNNNRTG 1 cut(s) 132
Sse9I AATT 4 cut(s) 100, 248, 356, 653
SsiI CCGC 2 cut(s) 536, 554
SspI AATATT 2 cut(s) 76, 257
SspMI CTAG 2 cut(s) 35, 161
StyD4I CCNGG 1 cut(s) 372
TaaI ACNGT 6 cut(s) 42, 134, 329, 478, 641, 778
TaiI ACGT 2 cut(s) 277, 435
TaqI TCGA 1 cut(s) 760
TasI AATT 4 cut(s) 100, 248, 356, 653
TatI WGTACW 2 cut(s) 17, 42
TfiI GAWTC 2 cut(s) 319, 578
Tru1I TTAA 3 cut(s) 387, 614, 725
Tru9I TTAA 3 cut(s) 387, 614, 725
TscAI CASTG 2 cut(s) 93, 139
TseI GCWGC 1 cut(s) 280
TspDTI ATGAA 2 cut(s) 401, 682
TspGWI ACGGA 1 cut(s) 723
TspRI CASTG 2 cut(s) 93, 139
Tth111I GACNNNGTC 1 cut(s) 745
VpaK11BI GGWCC 1 cut(s) 660
VspI ATTAAT 1 cut(s) 614
XapI RAATTY 1 cut(s) 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 2 cut(s) 35, 161
ZraI GACGTC 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.