FvH4_3g01050

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016777
Physical Location & Seq
Reverse (-)
527581 .. 528867
1287 bp
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UTR
Exon/CDS
Intron
FvH4_3g01050.t1

Sequence Viewer

Length: 549 bp
ATGGCTGGATTGAAGGTGGTGTTTGTTGTCTTGGCCATCAGTTTCAGCCTTGGAGGGAACTGGGTCGGGGCTCAAGTCCACCATGTCGTCGGAGGAGATCGTGGGTGGGATCCTGCTAACTCCGATCTCACTTCTTGGTCCTCCGGCAAAAGCTTTATGGTCGGAGACACAATCTGGTTTGCATACTCTGCAGCGCATGGGTTCATAGCTGAAGTGAAAAGCAAGGAGGAGTTCGATTCTTGTGATGTAAGCAATCCGATCAGAATGTTCAAAGATGGGTTGGACAGCACCCCAATGGACAAGGAAGGACTACGCTACTTCACAAGCAGTAACCCTGAGAGCTGCAAGAATGGCCTCAAGTTGCACGTTCAAGTTGTGCCTCGTCAGGACCGTCCTCAAACTGTTATGCCGGTAGTGGCGACATCCGAGATCTCTGCATTGGCTGCAGCTGAAGCGCCATCAACTCCTTCCGGTTCAGCTCATCTCGGTTCAAGTCTCGTTTTGTTGTCAATTGGGTTTGCTTTGCTAGGTTGTGTAATGGGACTCTAG

Protein Analysis

183

Amino Acids

19.26

Weight (kDa)

5.66

Isoelectric Point (pI)

28.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 118 5.3e-19 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016777)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01050
malus_domestica MD05G1357200.v1.1
prunus_persica Prupe.4G011400_v2.0.a1
pyrus_communis pycom10g28040
rosa_chinensis RchiOBHm_Chr5g0001591
rosa_laevigata RLG00000030945
rosa_multiflora Rmu_sc0041139.1_g000001
rosa_roxburghii Rroxscaffold_1G00074730
rosa_rugosa Rorug04G0391200
rosa_samantha Rh5AG012600 Rh5BG015500 Rh5CG013600 Rh5DG013400
rosa_wichuraiana Rw5G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 104, 117
AcoI YGGCCR 1 cut(s) 33
AcuI CTGAAG 2 cut(s) 231, 471
AgsI TTSAA 4 cut(s) 13, 271, 371, 492
AluBI AGCT 5 cut(s) 153, 209, 342, 449, 479
AluI AGCT 5 cut(s) 153, 209, 342, 449, 479
Alw26I GTCTC 2 cut(s) 159, 500
AlwI GGATC 2 cut(s) 104, 117
AoxI GGCC 2 cut(s) 33, 352
ApeKI GCWGC 4 cut(s) 191, 342, 443, 446
AspLEI GCGC 2 cut(s) 196, 457
AspS9I GGNCC 2 cut(s) 138, 388
AvaII GGWCC 2 cut(s) 138, 388
BalI TGGCCA 1 cut(s) 35
BamHI GGATCC 1 cut(s) 109
BanII GRGCYC 1 cut(s) 73
BbvI GCAGC 4 cut(s) 203, 329, 430, 458
BccI CCATC 3 cut(s) 44, 269, 466
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BcoDI GTCTC 2 cut(s) 159, 500
BfaI CTAG 2 cut(s) 527, 547
BfmI CTRYAG 2 cut(s) 189, 444
BfoI RGCGCY 1 cut(s) 458
BglII AGATCT 1 cut(s) 429
BisI GCNGC 4 cut(s) 192, 343, 444, 447
BlsI GCNGC 4 cut(s) 193, 344, 445, 448
Bme18I GGWCC 2 cut(s) 138, 388
BmgT120I GGNCC 2 cut(s) 138, 388
BmiI GGNNCC 1 cut(s) 111
BmrI ACTGGG 1 cut(s) 70
BmuI ACTGGG 1 cut(s) 70
BpuEI CTTGAG 2 cut(s) 57, 341
BsaJI CCNNGG 1 cut(s) 49
BsaWI WCCGGW 1 cut(s) 470
Bse118I RCCGGY 1 cut(s) 409
Bse1I ACTGG 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 1 cut(s) 422
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 65
BseRI GAGGAG 2 cut(s) 108, 242
BseXI GCAGC 4 cut(s) 203, 329, 430, 458
BshFI GGCC 2 cut(s) 35, 354
BsiSI CCGG 3 cut(s) 144, 410, 471
BsmAI GTCTC 2 cut(s) 159, 500
BsnI GGCC 2 cut(s) 35, 354
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 5 cut(s) 97, 109, 124, 258, 429
BspANI GGCC 2 cut(s) 35, 354
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 111
BspMAI CTGCAG 2 cut(s) 193, 448
BspPI GGATC 2 cut(s) 104, 117
BsrFI RCCGGY 1 cut(s) 409
BsrI ACTGG 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 409
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 5 cut(s) 97, 109, 124, 258, 429
BssT1I CCWWGG 1 cut(s) 49
Bst4CI ACNGT 2 cut(s) 392, 403
BstAPI GCANNNNNTGC 2 cut(s) 188, 443
BstDEI CTNAG 1 cut(s) 336
BstF5I GGATG 1 cut(s) 422
BstH2I RGCGCY 1 cut(s) 458
BstHHI GCGC 2 cut(s) 196, 457
BstKTI GATC 5 cut(s) 100, 112, 127, 261, 432
BstMAI GTCTC 2 cut(s) 159, 500
BstMBI GATC 5 cut(s) 97, 109, 124, 258, 429
BstMWI GCNNNNNNNGC 4 cut(s) 188, 351, 443, 452
BstSFI CTRYAG 2 cut(s) 189, 444
BstV1I GCAGC 4 cut(s) 203, 329, 430, 458
BstX2I RGATCY 2 cut(s) 109, 429
BstYI RGATCY 2 cut(s) 109, 429
BsuRI GGCC 2 cut(s) 35, 354
BtsCI GGATG 1 cut(s) 422
CfoI GCGC 2 cut(s) 196, 457
Cfr10I RCCGGY 1 cut(s) 409
Cfr13I GGNCC 2 cut(s) 138, 388
CviAII CATG 2 cut(s) 83, 197
DdeI CTNAG 1 cut(s) 336
DpnI GATC 5 cut(s) 99, 111, 126, 260, 431
DpnII GATC 5 cut(s) 97, 109, 124, 258, 429
EaeI YGGCCR 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 49
Eco24I GRGCYC 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 138, 388
Eco57I CTGAAG 2 cut(s) 231, 471
EcoT14I CCWWGG 1 cut(s) 49
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 2 cut(s) 86, 200
FaiI YATR 6 cut(s) 84, 158, 184, 198, 206, 407
FatI CATG 2 cut(s) 82, 196
Fnu4HI GCNGC 4 cut(s) 192, 343, 444, 447
FokI GGATG 1 cut(s) 409
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 4 cut(s) 192, 343, 444, 447
FspBI CTAG 2 cut(s) 527, 547
GlaI GCGC 2 cut(s) 195, 456
GluI GCNGC 4 cut(s) 192, 343, 444, 447
HaeII RGCGCY 1 cut(s) 458
HaeIII GGCC 2 cut(s) 35, 354
HapII CCGG 3 cut(s) 144, 410, 471
HhaI GCGC 2 cut(s) 196, 457
Hin1II CATG 2 cut(s) 86, 200
Hin6I GCGC 2 cut(s) 194, 455
HinP1I GCGC 2 cut(s) 194, 455
HindIII AAGCTT 1 cut(s) 151
HinfI GANTC 2 cut(s) 236, 543
HpaII CCGG 3 cut(s) 144, 410, 471
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 6 cut(s) 92, 124, 164, 258, 263, 427
Hpy188III TCNNGA 1 cut(s) 386
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 7, 299, 477
HpyCH4III ACNGT 2 cut(s) 392, 403
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 6 cut(s) 182, 191, 345, 364, 437, 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 351, 443, 452
HpyF3I CTNAG 1 cut(s) 336
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 2 cut(s) 86, 200
HspAI GCGC 2 cut(s) 194, 455
Kzo9I GATC 5 cut(s) 97, 109, 124, 258, 429
LpnPI CCDG 8 cut(s) 46, 126, 157, 160, 348, 371, 423, 484
Lsp1109I GCAGC 4 cut(s) 203, 329, 430, 458
MaeI CTAG 2 cut(s) 527, 547
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 1 cut(s) 329
MalI GATC 5 cut(s) 99, 111, 126, 260, 431
MboI GATC 5 cut(s) 97, 109, 124, 258, 429
MfeI CAATTG 1 cut(s) 510
MflI RGATCY 2 cut(s) 109, 429
MhlI GDGCHC 1 cut(s) 73
MlsI TGGCCA 1 cut(s) 35
MluCI AATT 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 35
MlyI GAGTC 1 cut(s) 537
MmeI TCCRAC 3 cut(s) 70, 142, 261
MnlI CCTC 7 cut(s) 47, 86, 151, 220, 365, 390, 405
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
MslI CAYNNNNRTG 1 cut(s) 293
Msp20I TGGCCA 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 3 cut(s) 144, 410, 471
MunI CAATTG 1 cut(s) 510
MwoI GCNNNNNNNGC 4 cut(s) 188, 351, 443, 452
NdeII GATC 5 cut(s) 97, 109, 124, 258, 429
NlaIII CATG 2 cut(s) 86, 200
NlaIV GGNNCC 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 388
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 4 cut(s) 193, 344, 445, 448
PleI GAGTC 1 cut(s) 537
PpsI GAGTC 1 cut(s) 537
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 2 cut(s) 138, 388
PstI CTGCAG 2 cut(s) 193, 448
PsuI RGATCY 2 cut(s) 109, 429
PvuII CAGCTG 1 cut(s) 449
RseI CAYNNNNRTG 1 cut(s) 293
SatI GCNGC 4 cut(s) 192, 343, 444, 447
Sau3AI GATC 5 cut(s) 97, 109, 124, 258, 429
Sau96I GGNCC 2 cut(s) 138, 388
SchI GAGTC 1 cut(s) 537
SduI GDGCHC 1 cut(s) 73
SetI ASST 8 cut(s) 18, 155, 211, 344, 369, 451, 481, 532
SfcI CTRYAG 2 cut(s) 189, 444
SinI GGWCC 2 cut(s) 138, 388
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 2 cut(s) 72, 356
SmoI CTYRAG 2 cut(s) 72, 356
Sse9I AATT 1 cut(s) 510
SspMI CTAG 2 cut(s) 527, 547
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 2 cut(s) 392, 403
TaiI ACGT 1 cut(s) 369
TaqI TCGA 1 cut(s) 234
TasI AATT 1 cut(s) 510
TfiI GAWTC 1 cut(s) 236
TseI GCWGC 4 cut(s) 191, 342, 443, 446
TspDTI ATGAA 1 cut(s) 193
VpaK11BI GGWCC 2 cut(s) 138, 388
XspI CTAG 2 cut(s) 527, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.