RLG00000030945

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016777
Physical Location & Seq
Reverse (-)
831554 .. 832325
772 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030945

Sequence Viewer

Length: 531 bp
ATGGCTGGATTGAAGGTGGTGTTTGTTGTTTTGGCTATCAGTTTCAGCCTTGGAGGGAACTGGGTCGGTGCTCAAGTCCACCATGTCGTCGGAGGAGATCGTGGCTGGGATCCGGCTAATTCCGATCTCACTTCTTGGTCCTCCGGCAAAAGCTTTATGGTCGGAGACACACTCTGGTTTGCATACTCTGCAGCACATGGGTTCATAGCAGAAGTGAAAAGCAAGGAGGAATTCGAATCTTGTGATGTGAGCAATCCGATCAGAATGTTCACAGATGGCTTGGATAGCACCCCAATGGACAAGGAAGGACTCCGCTACTTCACAAGCAGTAACCCTGAGAGCTGCAAGAATGGCCTCAAGTTACACGTTCAAGTCGTGCCTCATCAGGATCGATCTCAAACTGCAATGCCGATAGTGGCGACATCCAAGATCTCTGCATTGGCTGCAGGGCCAACTACTCCTTCTGGTTCAGCTCATCTCAGTTCAAGTCTCATTTTGTTGTCATTGGGTTTACTTTGCCATGTTATGTAA

Protein Analysis

177

Amino Acids

18.74

Weight (kDa)

6.12

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 118 7.4e-20 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016777)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01050
malus_domestica MD05G1357200.v1.1
prunus_persica Prupe.4G011400_v2.0.a1
pyrus_communis pycom10g28040
rosa_chinensis RchiOBHm_Chr5g0001591
rosa_laevigata RLG00000030945
rosa_multiflora Rmu_sc0041139.1_g000001
rosa_roxburghii Rroxscaffold_1G00074730
rosa_rugosa Rorug04G0391200
rosa_samantha Rh5AG012600 Rh5BG015500 Rh5CG013600 Rh5DG013400
rosa_wichuraiana Rw5G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 313
AclWI GGATC 3 cut(s) 104, 117, 396
AcsI RAATTY 1 cut(s) 230
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 3 cut(s) 13, 371, 486
AjuI GAANNNNNNNTTGG 2 cut(s) 445, 477
AluBI AGCT 3 cut(s) 153, 342, 473
AluI AGCT 3 cut(s) 153, 342, 473
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 2 cut(s) 159, 494
AlwI GGATC 3 cut(s) 104, 117, 396
AoxI GGCC 2 cut(s) 352, 449
ApeKI GCWGC 3 cut(s) 191, 342, 443
ApoI RAATTY 1 cut(s) 230
AspS9I GGNCC 2 cut(s) 138, 449
AsuII TTCGAA 1 cut(s) 234
AvaII GGWCC 1 cut(s) 138
BamHI GGATCC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 3 cut(s) 203, 329, 430
BccI CCATC 1 cut(s) 269
BcoDI GTCTC 2 cut(s) 159, 494
BfmI CTRYAG 2 cut(s) 189, 444
BglII AGATCT 1 cut(s) 429
BisI GCNGC 3 cut(s) 192, 343, 444
BlsI GCNGC 3 cut(s) 193, 344, 445
Bme18I GGWCC 1 cut(s) 138
BmgT120I GGNCC 2 cut(s) 138, 449
BmiI GGNNCC 1 cut(s) 111
BmrI ACTGGG 1 cut(s) 70
BmuI ACTGGG 1 cut(s) 70
BplI GAGNNNNNCTC 2 cut(s) 156, 188
Bpu14I TTCGAA 1 cut(s) 234
BpuEI CTTGAG 2 cut(s) 57, 341
Bsa29I ATCGAT 1 cut(s) 391
BsaJI CCNNGG 1 cut(s) 49
Bse1I ACTGG 1 cut(s) 65
Bse3DI GCAATG 1 cut(s) 411
BseCI ATCGAT 1 cut(s) 391
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 1 cut(s) 422
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 2 cut(s) 327, 493
BseNI ACTGG 1 cut(s) 65
BseRI GAGGAG 1 cut(s) 108
BseXI GCAGC 3 cut(s) 203, 329, 430
BseYI CCCAGC 1 cut(s) 105
BshFI GGCC 2 cut(s) 354, 451
BshVI ATCGAT 1 cut(s) 391
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 2 cut(s) 113, 144
BsmAI GTCTC 2 cut(s) 159, 494
BsnI GGCC 2 cut(s) 354, 451
Bsp119I TTCGAA 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
BspACI CCGC 1 cut(s) 313
BspANI GGCC 2 cut(s) 354, 451
BspCNI CTCAG 2 cut(s) 328, 492
BspDI ATCGAT 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 111
BspMAI CTGCAG 2 cut(s) 193, 448
BspPI GGATC 3 cut(s) 104, 117, 396
BspT104I TTCGAA 1 cut(s) 234
BsrDI GCAATG 1 cut(s) 411
BsrI ACTGG 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
BssT1I CCWWGG 1 cut(s) 49
BstAPI GCANNNNNTGC 2 cut(s) 188, 443
BstBI TTCGAA 1 cut(s) 234
BstDEI CTNAG 2 cut(s) 336, 479
BstF5I GGATG 1 cut(s) 422
BstKTI GATC 7 cut(s) 100, 112, 127, 261, 391, 395, 432
BstMAI GTCTC 2 cut(s) 159, 494
BstMBI GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
BstMWI GCNNNNNNNGC 4 cut(s) 188, 285, 351, 443
BstSFI CTRYAG 2 cut(s) 189, 444
BstV1I GCAGC 3 cut(s) 203, 329, 430
BstX2I RGATCY 2 cut(s) 109, 429
BstYI RGATCY 2 cut(s) 109, 429
Bsu15I ATCGAT 1 cut(s) 391
BsuRI GGCC 2 cut(s) 354, 451
BsuTUI ATCGAT 1 cut(s) 391
BtsCI GGATG 1 cut(s) 422
Cfr13I GGNCC 2 cut(s) 138, 449
ClaI ATCGAT 1 cut(s) 391
CviAII CATG 3 cut(s) 83, 197, 521
DdeI CTNAG 2 cut(s) 336, 479
DpnI GATC 7 cut(s) 99, 111, 126, 260, 390, 394, 431
DpnII GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
Eco130I CCWWGG 1 cut(s) 49
Eco47I GGWCC 1 cut(s) 138
EcoRI GAATTC 1 cut(s) 230
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 3 cut(s) 86, 200, 524
FaiI YATR 7 cut(s) 84, 158, 184, 198, 206, 522, 527
FatI CATG 3 cut(s) 82, 196, 520
Fnu4HI GCNGC 3 cut(s) 192, 343, 444
FokI GGATG 1 cut(s) 409
Fsp4HI GCNGC 3 cut(s) 192, 343, 444
GluI GCNGC 3 cut(s) 192, 343, 444
GsaI CCCAGC 1 cut(s) 109
HaeIII GGCC 2 cut(s) 354, 451
HapII CCGG 2 cut(s) 113, 144
Hin1II CATG 3 cut(s) 86, 200, 524
HindIII AAGCTT 1 cut(s) 151
HinfI GANTC 2 cut(s) 236, 309
HpaII CCGG 2 cut(s) 113, 144
Hpy166II GTNNAC 3 cut(s) 79, 270, 512
Hpy188I TCNGA 5 cut(s) 92, 124, 164, 258, 263
Hpy188III TCNNGA 1 cut(s) 386
Hpy8I GTNNAC 3 cut(s) 79, 270, 512
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 7, 299, 471
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 6 cut(s) 182, 191, 345, 404, 437, 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 285, 351, 443
HpyF3I CTNAG 2 cut(s) 336, 479
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 3 cut(s) 86, 200, 524
Kzo9I GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
LpnPI CCDG 9 cut(s) 46, 91, 126, 157, 160, 348, 371, 432, 450
Lsp1109I GCAGC 3 cut(s) 203, 329, 430
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 2 cut(s) 329, 360
MalI GATC 7 cut(s) 99, 111, 126, 260, 390, 394, 431
MboI GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
MflI RGATCY 2 cut(s) 109, 429
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 2 cut(s) 118, 230
MlyI GAGTC 1 cut(s) 303
MmeI TCCRAC 2 cut(s) 70, 142
MnlI CCTC 6 cut(s) 47, 86, 151, 220, 365, 390
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 2 cut(s) 113, 144
MwoI GCNNNNNNNGC 4 cut(s) 188, 285, 351, 443
NdeII GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
NlaIII CATG 3 cut(s) 86, 200, 524
NlaIV GGNNCC 1 cut(s) 111
NspV TTCGAA 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 3 cut(s) 193, 344, 445
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
PspFI CCCAGC 1 cut(s) 105
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 2 cut(s) 138, 449
PstI CTGCAG 2 cut(s) 193, 448
PsuI RGATCY 2 cut(s) 109, 429
RseI CAYNNNNRTG 1 cut(s) 293
SatI GCNGC 3 cut(s) 192, 343, 444
Sau3AI GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
Sau96I GGNCC 2 cut(s) 138, 449
SchI GAGTC 1 cut(s) 303
SduI GDGCHC 1 cut(s) 73
SetI ASST 5 cut(s) 18, 155, 344, 369, 475
SfcI CTRYAG 2 cut(s) 189, 444
SfuI TTCGAA 1 cut(s) 234
SinI GGWCC 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 2 cut(s) 72, 356
SmoI CTYRAG 2 cut(s) 72, 356
Sse9I AATT 2 cut(s) 118, 230
SsiI CCGC 1 cut(s) 313
StyI CCWWGG 1 cut(s) 49
TaiI ACGT 1 cut(s) 369
TaqI TCGA 2 cut(s) 234, 391
TasI AATT 2 cut(s) 118, 230
TfiI GAWTC 1 cut(s) 236
TseI GCWGC 3 cut(s) 191, 342, 443
TspDTI ATGAA 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 138
XapI RAATTY 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.