Rh5CG013600

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0016777
Physical Location & Seq
Reverse (-)
834009 .. 834784
776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG013600.1

Sequence Viewer

Length: 549 bp
ATGGCTGGATTGAAGGTGGTGTTTGTTGTTTTGGCCATCAGTTTCAGCCTTGGAGGGAACTGGGTCGGAGCTCAAGTCCACCATGTCGTCGGAGGAGATCGTGGCTGGGATCCTGCTAATTCCGATCTCACGTCCTGGTCCTCCGGCAAAAGCTTTATGGTCGGAGACACACTCTGGTTTGCATACTCTGCAGCACATGGGTTCATAGCAGAAGTGAAAAGCAAGGAGGAATTCGAATCTTGTGATGTGAGCAATCCGATCAGAATGTTCACAGATGGCTTGGATAGCACCCCAATGGACAAGGAAGGACTCCGCTACTTCACAAGCAGTAACCCTGAGAGCTGCAAGAATGGCCTCAAGTTACACGTTCAAGTTTTGCCTCATCAGGATCGATCTCAAACTGTAATGCCGATAGTGGCGACATCCGAGATCTCTGCACTGGCTGCAGCTGAAGGGCCAACAACTCCTTCTGGTTCAGCTCATCTCAGTTCAAGTCTCATTTTGTTGTCATTTGCGTTTGCTTTGCTATGTTTTGTAATGGGTCAGTAG

Protein Analysis

182

Amino Acids

19.44

Weight (kDa)

5.39

Isoelectric Point (pI)

29.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 118 8.1e-20 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016777)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01050
malus_domestica MD05G1357200.v1.1
prunus_persica Prupe.4G011400_v2.0.a1
pyrus_communis pycom10g28040
rosa_chinensis RchiOBHm_Chr5g0001591
rosa_laevigata RLG00000030945
rosa_multiflora Rmu_sc0041139.1_g000001
rosa_roxburghii Rroxscaffold_1G00074730
rosa_rugosa Rorug04G0391200
rosa_samantha Rh5AG012600 Rh5BG015500 Rh5CG013600 Rh5DG013400
rosa_wichuraiana Rw5G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 313
AclWI GGATC 3 cut(s) 104, 117, 396
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 1 cut(s) 230
AcuI CTGAAG 1 cut(s) 471
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 3 cut(s) 13, 371, 492
AjiI CACGTC 1 cut(s) 132
AjnI CCWGG 1 cut(s) 134
AjuI GAANNNNNNNTTGG 2 cut(s) 451, 483
AluBI AGCT 5 cut(s) 71, 153, 342, 449, 479
AluI AGCT 5 cut(s) 71, 153, 342, 449, 479
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 2 cut(s) 159, 500
AlwI GGATC 3 cut(s) 104, 117, 396
AoxI GGCC 3 cut(s) 33, 352, 455
ApeKI GCWGC 4 cut(s) 191, 342, 443, 446
ApoI RAATTY 1 cut(s) 230
AspS9I GGNCC 2 cut(s) 138, 455
AsuII TTCGAA 1 cut(s) 234
AvaII GGWCC 1 cut(s) 138
BalI TGGCCA 1 cut(s) 35
BamHI GGATCC 1 cut(s) 109
BanII GRGCYC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 4 cut(s) 203, 329, 430, 458
BccI CCATC 2 cut(s) 44, 269
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 159, 500
BfmI CTRYAG 2 cut(s) 189, 444
BglII AGATCT 1 cut(s) 429
BisI GCNGC 4 cut(s) 192, 343, 444, 447
BlsI GCNGC 4 cut(s) 193, 344, 445, 448
Bme1390I CCNGG 1 cut(s) 136
Bme18I GGWCC 1 cut(s) 138
BmgBI CACGTC 1 cut(s) 132
BmgT120I GGNCC 2 cut(s) 138, 455
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 136
BmrI ACTGGG 1 cut(s) 70
BmuI ACTGGG 1 cut(s) 70
BplI GAGNNNNNCTC 2 cut(s) 156, 188
Bpu14I TTCGAA 1 cut(s) 234
BpuEI CTTGAG 2 cut(s) 57, 341
Bsa29I ATCGAT 1 cut(s) 391
BsaJI CCNNGG 1 cut(s) 49
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bse1I ACTGG 2 cut(s) 65, 444
BseBI CCWGG 1 cut(s) 136
BseCI ATCGAT 1 cut(s) 391
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 1 cut(s) 422
BseMII CTCAG 2 cut(s) 327, 499
BseNI ACTGG 2 cut(s) 65, 444
BseRI GAGGAG 1 cut(s) 108
BseXI GCAGC 4 cut(s) 203, 329, 430, 458
BseYI CCCAGC 1 cut(s) 105
BsgI GTGCAG 1 cut(s) 420
BshFI GGCC 3 cut(s) 35, 354, 457
BshVI ATCGAT 1 cut(s) 391
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 144
BsmAI GTCTC 2 cut(s) 159, 500
BsnI GGCC 3 cut(s) 35, 354, 457
Bsp119I TTCGAA 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
BspACI CCGC 1 cut(s) 313
BspANI GGCC 3 cut(s) 35, 354, 457
BspCNI CTCAG 2 cut(s) 328, 498
BspDI ATCGAT 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 111
BspMAI CTGCAG 2 cut(s) 193, 448
BspPI GGATC 3 cut(s) 104, 117, 396
BspT104I TTCGAA 1 cut(s) 234
BsrI ACTGG 2 cut(s) 65, 444
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 136
Bst4CI ACNGT 1 cut(s) 403
BstAPI GCANNNNNTGC 2 cut(s) 188, 443
BstBI TTCGAA 1 cut(s) 234
BstDEI CTNAG 2 cut(s) 336, 485
BstF5I GGATG 1 cut(s) 422
BstKTI GATC 7 cut(s) 100, 112, 127, 261, 391, 395, 432
BstMAI GTCTC 2 cut(s) 159, 500
BstMBI GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
BstMWI GCNNNNNNNGC 4 cut(s) 188, 285, 351, 443
BstNI CCWGG 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 134
BstSFI CTRYAG 2 cut(s) 189, 444
BstV1I GCAGC 4 cut(s) 203, 329, 430, 458
BstX2I RGATCY 2 cut(s) 109, 429
BstYI RGATCY 2 cut(s) 109, 429
Bsu15I ATCGAT 1 cut(s) 391
BsuRI GGCC 3 cut(s) 35, 354, 457
BsuTUI ATCGAT 1 cut(s) 391
BtrI CACGTC 1 cut(s) 132
BtsCI GGATG 1 cut(s) 422
BtsIMutI CAGTG 1 cut(s) 437
Cfr13I GGNCC 2 cut(s) 138, 455
ClaI ATCGAT 1 cut(s) 391
CviAII CATG 2 cut(s) 83, 197
DdeI CTNAG 2 cut(s) 336, 485
DpnI GATC 7 cut(s) 99, 111, 126, 260, 390, 394, 431
DpnII GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
EaeI YGGCCR 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 49
Eco24I GRGCYC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 471
EcoICRI GAGCTC 1 cut(s) 71
EcoRI GAATTC 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 134
EcoT14I CCWWGG 1 cut(s) 49
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 2 cut(s) 86, 200
FaiI YATR 6 cut(s) 84, 158, 184, 198, 206, 529
FatI CATG 2 cut(s) 82, 196
Fnu4HI GCNGC 4 cut(s) 192, 343, 444, 447
FokI GGATG 1 cut(s) 409
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 4 cut(s) 192, 343, 444, 447
GluI GCNGC 4 cut(s) 192, 343, 444, 447
GsaI CCCAGC 1 cut(s) 109
HaeIII GGCC 3 cut(s) 35, 354, 457
HapII CCGG 1 cut(s) 144
Hin1II CATG 2 cut(s) 86, 200
HindIII AAGCTT 1 cut(s) 151
HinfI GANTC 2 cut(s) 236, 309
HpaII CCGG 1 cut(s) 144
Hpy166II GTNNAC 2 cut(s) 79, 270
Hpy188I TCNGA 7 cut(s) 68, 92, 124, 164, 258, 263, 427
Hpy188III TCNNGA 1 cut(s) 386
Hpy8I GTNNAC 2 cut(s) 79, 270
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 4 cut(s) 7, 299, 446, 477
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4IV ACGT 2 cut(s) 131, 366
HpyCH4V TGCA 5 cut(s) 182, 191, 345, 437, 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 285, 351, 443
HpyF3I CTNAG 2 cut(s) 336, 485
HpySE526I ACGT 2 cut(s) 131, 366
Hsp92II CATG 2 cut(s) 86, 200
Kzo9I GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
LmnI GCTCC 1 cut(s) 68
Lsp1109I GCAGC 4 cut(s) 203, 329, 430, 458
MaeII ACGT 2 cut(s) 131, 366
MaeIII GTNAC 2 cut(s) 329, 360
MalI GATC 7 cut(s) 99, 111, 126, 260, 390, 394, 431
MboI GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
MflI RGATCY 2 cut(s) 109, 429
MhlI GDGCHC 1 cut(s) 73
MlsI TGGCCA 1 cut(s) 35
MluCI AATT 2 cut(s) 118, 230
MluNI TGGCCA 1 cut(s) 35
MlyI GAGTC 1 cut(s) 303
MmeI TCCRAC 3 cut(s) 46, 70, 142
MnlI CCTC 6 cut(s) 47, 86, 151, 220, 365, 390
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
MslI CAYNNNNRTG 1 cut(s) 293
Msp20I TGGCCA 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 1 cut(s) 136
MvaI CCWGG 1 cut(s) 136
MwoI GCNNNNNNNGC 4 cut(s) 188, 285, 351, 443
NdeII GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
NlaIII CATG 2 cut(s) 86, 200
NlaIV GGNNCC 1 cut(s) 111
NspV TTCGAA 1 cut(s) 234
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 4 cut(s) 193, 344, 445, 448
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 134
PspFI CCCAGC 1 cut(s) 105
PspGI CCWGG 1 cut(s) 134
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 2 cut(s) 138, 455
PstI CTGCAG 2 cut(s) 193, 448
PsuI RGATCY 2 cut(s) 109, 429
PvuII CAGCTG 1 cut(s) 449
RseI CAYNNNNRTG 1 cut(s) 293
SacI GAGCTC 1 cut(s) 73
SatI GCNGC 4 cut(s) 192, 343, 444, 447
Sau3AI GATC 7 cut(s) 97, 109, 124, 258, 388, 392, 429
Sau96I GGNCC 2 cut(s) 138, 455
SchI GAGTC 1 cut(s) 303
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 1 cut(s) 73
SetI ASST 8 cut(s) 18, 73, 134, 155, 344, 369, 451, 481
SfcI CTRYAG 2 cut(s) 189, 444
SfuI TTCGAA 1 cut(s) 234
SinI GGWCC 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 2 cut(s) 72, 356
SmoI CTYRAG 2 cut(s) 72, 356
Sse9I AATT 2 cut(s) 118, 230
SsiI CCGC 1 cut(s) 313
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 1 cut(s) 134
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 1 cut(s) 403
TaiI ACGT 2 cut(s) 134, 369
TaqI TCGA 2 cut(s) 234, 391
TasI AATT 2 cut(s) 118, 230
TfiI GAWTC 1 cut(s) 236
TscAI CASTG 1 cut(s) 444
TseI GCWGC 4 cut(s) 191, 342, 443, 446
TspDTI ATGAA 1 cut(s) 193
TspRI CASTG 1 cut(s) 444
VpaK11BI GGWCC 1 cut(s) 138
XapI RAATTY 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.