MD05G1357200.v1.1

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016777
Physical Location & Seq
Forward (+)
47263109 .. 47264792
1684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1357200.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGCTGGAGCTGTAACATTGAAGGTGGCTGTTGCTGTTTTGGCGGTTGGTATCGCCGCCGTGAGCCTCGGAGGGAGCTTGGTTGGAGCACAAGTGCACCATGTGGTCGGAGGAGACCGCGGCTGGGATCCATCCTCCGACCTCGCTTCTTGGTCCTCGGGCAAAACATTTAGGGCGGGAGATAAGATGTGGCTAGCCTACTCCGCGGCACATGGGTACATAGCGGAAGTGACGAGTAAGGAGGAATTCGAGTCCTGCGATGTAACCAACCCAATCAGGATGTTCACGGACGGCCTGGATAGCATCTCGATAGACAATGAAGGACTCCGGTACTTCGCTAGCAGTAACGTCGAGAGCTGCAAGAAGGGCCTCAAACTACACGTGGAAGTTATGCCCCAATCATATCAAGCTCCTGAAACGTCCAACTCCCACGTGGCAGAATCAAAGGGCTCCGCGGTGGTTGCAGCTGAAGGGCCCAAAACCCCTTCTGGTTCGGCCCATCTCACTGCAAGCCTCATGGTGTTGTCATTTGGGTTGCTTTGTTATGCATTGGGTATTTAG

Protein Analysis

187

Amino Acids

19.32

Weight (kDa)

5.62

Isoelectric Point (pI)

28.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 44 - 123 2e-17 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016777)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01050
malus_domestica MD05G1357200.v1.1
prunus_persica Prupe.4G011400_v2.0.a1
pyrus_communis pycom10g28040
rosa_chinensis RchiOBHm_Chr5g0001591
rosa_laevigata RLG00000030945
rosa_multiflora Rmu_sc0041139.1_g000001
rosa_roxburghii Rroxscaffold_1G00074730
rosa_rugosa Rorug04G0391200
rosa_samantha Rh5AG012600 Rh5BG015500 Rh5CG013600 Rh5DG013400
rosa_wichuraiana Rw5G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 120, 206, 455
AclWI GGATC 2 cut(s) 122, 135
AcsI RAATTY 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 489
AcvI CACGTG 2 cut(s) 382, 433
AdeI CACNNNGTG 1 cut(s) 103
AfaI GTAC 2 cut(s) 218, 332
AfiI CCNNNNNNNGG 1 cut(s) 124
AflIII ACRYGT 1 cut(s) 379
AgsI TTSAA 1 cut(s) 22
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 5 cut(s) 11, 78, 357, 410, 467
AluI AGCT 5 cut(s) 11, 78, 357, 410, 467
Alw21I GWGCWC 2 cut(s) 91, 99
Alw26I GTCTC 1 cut(s) 108
Alw44I GTGCAC 1 cut(s) 95
AlwI GGATC 2 cut(s) 122, 135
Ama87I CYCGRG 1 cut(s) 157
AoxI GGCC 4 cut(s) 292, 367, 473, 495
ApaI GGGCCC 1 cut(s) 477
ApaLI GTGCAC 1 cut(s) 95
ApeKI GCWGC 2 cut(s) 357, 464
ApoI RAATTY 1 cut(s) 245
AspS9I GGNCC 5 cut(s) 153, 367, 473, 474, 496
AsuNHI GCTAGC 2 cut(s) 193, 338
AvaI CYCGRG 1 cut(s) 157
AvaII GGWCC 1 cut(s) 153
BaeGI GKGCMC 2 cut(s) 99, 477
BamHI GGATCC 1 cut(s) 127
BanII GRGCYC 2 cut(s) 452, 477
BbrPI CACGTG 2 cut(s) 382, 433
Bbv12I GWGCWC 2 cut(s) 91, 99
BbvI GCAGC 2 cut(s) 344, 476
BccI CCATC 2 cut(s) 139, 507
BceAI ACGGC 2 cut(s) 44, 307
BcgI CGANNNNNNTGC 2 cut(s) 331, 365
BciT130I CCWGG 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 108
BfaI CTAG 2 cut(s) 194, 339
BisI GCNGC 5 cut(s) 57, 121, 207, 358, 465
BlsI GCNGC 5 cut(s) 58, 122, 208, 359, 466
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 157
BmgT120I GGNCC 5 cut(s) 153, 367, 473, 474, 496
BmiI GGNNCC 3 cut(s) 129, 451, 475
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 1 cut(s) 312
BmtI GCTAGC 2 cut(s) 197, 342
BpmI CTGGAG 1 cut(s) 27
BsaAI YACGTR 2 cut(s) 382, 433
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 5 cut(s) 67, 118, 156, 204, 453
BsaWI WCCGGW 1 cut(s) 327
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 1 cut(s) 124
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 5 cut(s) 67, 118, 156, 204, 453
BseGI GGATG 2 cut(s) 131, 285
BseLI CCNNNNNNNGG 1 cut(s) 124
BseRI GAGGAG 1 cut(s) 126
BseSI GKGCMC 2 cut(s) 99, 477
BseXI GCAGC 2 cut(s) 344, 476
BseYI CCCAGC 1 cut(s) 123
Bsh1236I CGCG 3 cut(s) 120, 206, 455
BshFI GGCC 4 cut(s) 294, 369, 475, 497
BsiHKAI GWGCWC 2 cut(s) 91, 99
BsiHKCI CYCGRG 1 cut(s) 157
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 108
BsnI GGCC 4 cut(s) 294, 369, 475, 497
Bso31I GGTCTC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 157
Bsp120I GGGCCC 1 cut(s) 473
Bsp1286I GDGCHC 4 cut(s) 91, 99, 452, 477
Bsp143I GATC 1 cut(s) 127
BspANI GGCC 4 cut(s) 294, 369, 475, 497
BspFNI CGCG 3 cut(s) 120, 206, 455
BspLI GGNNCC 3 cut(s) 129, 451, 475
BspOI GCTAGC 2 cut(s) 197, 342
BspPI GGATC 2 cut(s) 122, 135
BspTNI GGTCTC 1 cut(s) 108
BssECI CCNNGG 5 cut(s) 67, 118, 156, 204, 453
BssMI GATC 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 296
BstBAI YACGTR 2 cut(s) 382, 433
BstC8I GCNNGC 3 cut(s) 195, 340, 511
BstDSI CCRYGG 3 cut(s) 118, 204, 453
BstF5I GGATG 2 cut(s) 131, 285
BstFNI CGCG 3 cut(s) 120, 206, 455
BstKTI GATC 1 cut(s) 130
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 1 cut(s) 127
BstMWI GCNNNNNNNGC 5 cut(s) 41, 203, 300, 366, 461
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 294
BstSLI GKGCMC 2 cut(s) 99, 477
BstUI CGCG 3 cut(s) 120, 206, 455
BstV1I GCAGC 2 cut(s) 344, 476
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BsuRI GGCC 4 cut(s) 294, 369, 475, 497
BtgI CCRYGG 3 cut(s) 118, 204, 453
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 2 cut(s) 131, 285
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 3 cut(s) 195, 340, 511
Cfr13I GGNCC 5 cut(s) 153, 367, 473, 474, 496
Cfr42I CCGCGG 3 cut(s) 121, 207, 456
Csp6I GTAC 2 cut(s) 217, 331
CviAII CATG 3 cut(s) 101, 212, 517
CviQI GTAC 2 cut(s) 217, 331
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
DraIII CACNNNGTG 1 cut(s) 103
Eco24I GRGCYC 2 cut(s) 452, 477
Eco31I GGTCTC 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 489
Eco72I CACGTG 2 cut(s) 382, 433
Eco88I CYCGRG 1 cut(s) 157
EcoO109I RGGNCCY 2 cut(s) 367, 473
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 294
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 2 cut(s) 452, 477
FaeI CATG 3 cut(s) 104, 215, 520
FaiI YATR 7 cut(s) 102, 213, 221, 392, 403, 518, 546
FatI CATG 3 cut(s) 100, 211, 516
FauI CCCGC 1 cut(s) 169
Fnu4HI GCNGC 5 cut(s) 57, 121, 207, 358, 465
FokI GGATG 2 cut(s) 118, 292
FriOI GRGCYC 2 cut(s) 452, 477
Fsp4HI GCNGC 5 cut(s) 57, 121, 207, 358, 465
FspBI CTAG 2 cut(s) 194, 339
GluI GCNGC 5 cut(s) 57, 121, 207, 358, 465
GsaI CCCAGC 1 cut(s) 127
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 4 cut(s) 294, 369, 475, 497
HapII CCGG 1 cut(s) 328
Hin1II CATG 3 cut(s) 104, 215, 520
HinfI GANTC 3 cut(s) 251, 324, 440
HpaII CCGG 1 cut(s) 328
Hpy166II GTNNAC 2 cut(s) 97, 285
Hpy188I TCNGA 3 cut(s) 71, 110, 139
Hpy188III TCNNGA 4 cut(s) 277, 307, 352, 413
Hpy8I GTNNAC 2 cut(s) 97, 285
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 5 cut(s) 16, 314, 358, 464, 495
HpyCH4IV ACGT 4 cut(s) 348, 381, 419, 432
HpyCH4V TGCA 5 cut(s) 97, 360, 464, 509, 548
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 203, 300, 366, 461
HpySE526I ACGT 4 cut(s) 348, 381, 419, 432
Hsp92II CATG 3 cut(s) 104, 215, 520
KspI CCGCGG 3 cut(s) 121, 207, 456
Kzo9I GATC 1 cut(s) 127
LmnI GCTCC 5 cut(s) 8, 75, 86, 415, 455
LpnPI CCDG 8 cut(s) 109, 262, 268, 281, 308, 341, 426, 474
Lsp1109I GCAGC 2 cut(s) 344, 476
LweI GCATC 1 cut(s) 312
MaeI CTAG 2 cut(s) 194, 339
MaeII ACGT 4 cut(s) 348, 381, 419, 432
MaeIII GTNAC 4 cut(s) 13, 229, 262, 344
MalI GATC 1 cut(s) 129
MboI GATC 1 cut(s) 127
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 4 cut(s) 91, 99, 452, 477
MluCI AATT 1 cut(s) 245
MlyI GAGTC 2 cut(s) 260, 318
MmeI TCCRAC 4 cut(s) 64, 88, 162, 447
MnlI CCTC 9 cut(s) 65, 77, 104, 145, 152, 166, 235, 380, 524
Mph1103I ATGCAT 1 cut(s) 550
MspA1I CMGCKG 4 cut(s) 120, 206, 455, 467
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 3 cut(s) 120, 206, 455
MwoI GCNNNNNNNGC 5 cut(s) 41, 203, 300, 366, 461
NdeII GATC 1 cut(s) 127
NheI GCTAGC 2 cut(s) 193, 338
NlaIII CATG 3 cut(s) 104, 215, 520
NlaIV GGNNCC 3 cut(s) 129, 451, 475
NmuCI GTSAC 1 cut(s) 229
NsiI ATGCAT 1 cut(s) 550
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 1 cut(s) 440
PkrI GCNGC 5 cut(s) 58, 122, 208, 359, 466
PleI GAGTC 2 cut(s) 259, 318
PmaCI CACGTG 2 cut(s) 382, 433
PmlI CACGTG 2 cut(s) 382, 433
PpsI GAGTC 2 cut(s) 259, 318
Ppu21I YACGTR 2 cut(s) 382, 433
Psp6I CCWGG 1 cut(s) 294
PspCI CACGTG 2 cut(s) 382, 433
PspFI CCCAGC 1 cut(s) 123
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 129, 451, 475
PspOMI GGGCCC 1 cut(s) 473
PspPI GGNCC 5 cut(s) 153, 367, 473, 474, 496
PsuI RGATCY 1 cut(s) 127
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 2 cut(s) 218, 332
RsaNI GTAC 2 cut(s) 217, 331
SacII CCGCGG 3 cut(s) 121, 207, 456
SatI GCNGC 5 cut(s) 57, 121, 207, 358, 465
Sau3AI GATC 1 cut(s) 127
Sau96I GGNCC 5 cut(s) 153, 367, 473, 474, 496
SchI GAGTC 2 cut(s) 260, 318
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 4 cut(s) 91, 99, 452, 477
SfaNI GCATC 1 cut(s) 312
Sfr303I CCGCGG 3 cut(s) 121, 207, 456
SgrBI CCGCGG 3 cut(s) 121, 207, 456
SinI GGWCC 1 cut(s) 153
Sse9I AATT 1 cut(s) 245
SspMI CTAG 2 cut(s) 194, 339
StyD4I CCNGG 1 cut(s) 294
TaiI ACGT 4 cut(s) 351, 384, 422, 435
TaqI TCGA 3 cut(s) 249, 308, 351
TasI AATT 1 cut(s) 245
TauI GCSGC 3 cut(s) 59, 123, 209
TfiI GAWTC 1 cut(s) 440
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 2 cut(s) 357, 464
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 333
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 1 cut(s) 511
VneI GTGCAC 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 153
XapI RAATTY 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
XspI CTAG 2 cut(s) 194, 339
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.