Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0006919

Physical Location & Seq

Genomic Coordinates Reverse (-)

2687666 .. 2689429

Gene Structure 1764 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g04680.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGCTTCTCCTCTACCACCCAGCTGTACTGCTACAATCTTCTGCCCTAACACTTCACTGATGACCTCAAACACTTCTGTCACTCCCTTCTTCACCAAGAAATTCAAATCCCAAAAACCTTCATTGGTCATAAGACCTAAGCAAGGCATTGTATGCAAGGCAACCAACAACAATGACCAAAATAAAGATGCTGTGGGAAAATTGGACAGGAGAAACATGCTCATTGGCTTGGGTGCTGGCGGTCTATACGGCACGGCGGGTCTCGAAACCAATCCGTTTGCATTCGCCGCGCCCGTGCCACCACCCGACCTAGCCACATGCGGTCCGGCGGACAAACCTGACGGTACAACCATCGATTGTTGCCCGCCAAAAACCACCACAATCATCGACTTTAAGCTACCTGATCCGGGTCCTATGCGCACTAGGCTAGCTGCCCAGAACGTCGCAAAAGACCCAGTATACTTGGCAAAATACAAGAAGGCCATTGAGCTCATGCGGGCCTTACCAGACGACGACCCGCGAAGCCTGGCCCAGCAGGCCATGGTTCACTGTTCGTACTGCGACGGTGGTTATCCCATGGCCGGGTTTTCCGACCTCGAAATCCAAGTTCACTTCTGCTGGCTCTTCTACCCTTGGCACCGTTTGTACCTCTACTTCTACGAGAAGATCATGTGCAAGCTCATCGACGATCCCACTTTCGCTCTTCCCTTCTGGAACTGGGACGCTCCGGCCGGCATGTACATCCCCTCCATTTTCACCGACACCACCTCCTCTCTCTACGACCAATACCGGAACGCAGCGCATCAACCTCCGAAGCTTCTAGACCTCAACTACGGTGGCACCGATGACGACACGGATGACAAGACTAGAATCAGAGAGAACCTCACCACCATGTACCAACAAATGATCTCAAAAGCCACATCTCACCGCCTCTTCTTCGGTGAGCCCTACAGCGCCGGAGACGAACCGAACCCCGGCGCCGGAAACATCGAGAGTATTCCGCATAATAACATCCATCTCTGGACCGGAGATCCAACTCAGACTAACGGAGAGGACATGGGAGCTTTCTACTCAGCAGGGAGGGATCCTATCTTTTACTCTCACCATGCCAACGTAGACCGCATGTGGTCTATCTACAAGGCACGAGGTGGGACTGATATAACCAAAAAGGATTGGCTTGACACCGAGTTCTTGTTCTACGATGAGAACAAGAATCTGGTCCGAGTTAAAGTACGTGACTCGCTAGACGAGTCGAAACTAGGGTACAAGTACCAGGACGTGGAGATTCCGTGGCTGAACTCGAAGCCTACGGCAAGGAAATCGAAGAATAAAAGAAAGGCGGCGGTTTCCTCAGCGGACTTGACTTCGAAGTTTCCGGCGACGTTGAGTGAGACTATTAGTGTTGAGGTGGCGCGGCCGTCGGCGGCTAAGAGGACAACGGCGGAGAAGACGAAGGAGGAGGAGGTGTTGGTGATCAGTGGTATCGAGTTCGCCGGAAGCGAGATGTTGAAGTTTGATGTGTACGTGAATGATGATGCGGATGAGGTGAGCGGGAAGGACAAGGCGGAGTTTGCCGGGAGTTTTGTGCATGTGCCGCATAGGGCGAACAAGAAGATCAAGACAAACTTGAGGTTGGGGATTACGAATCTACTGACGGATTTGGGGGCGGAGGAGGATAATAGCGTGGTGGTGACATTGGTGCCCAAGTTTGGAAAAGGAGCTATCATAGGTGGGTTCAAGATCGAGCTTATTTCTACCACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

588

Amino Acids

65.14

Weight (kDa)

6.3

Isoelectric Point (pI)

35.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	174 - 381	1.9e-35	Common central domain of tyrosinase
PPO1_DWL	PF12142	386 - 437	7.8e-27	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	458 - 583	4.2e-46	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g04680
malus_domestica	MD05G1319800.v1.1 MD05G1320100.v1.1 MD05G1320200.v1.1 MD05G1320500.v1.1 MD05G1320800.v1.1 MD10G1298300.v1.1
prunus_persica	Prupe.4G041500_v2.0.a1 Prupe.4G041700_v2.0.a1
pyrus_communis	pycom05g29530 pycom05g29560 pycom10g24890 pycom10g24910 pycom10g25140
rosa_chinensis	RchiOBHm_Chr5g0007621
rosa_laevigata	RLG00000031479
rosa_multiflora	Rmu_co8439317.1_g000001 Rmu_sc0011744.1_g000006 Rmu_sc0021005.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068730
rosa_rugosa	Rorug04G0429400
rosa_samantha	Rh5AG057500 Rh5BG054400 Rh5CG064400 Rh5DG053600
rosa_wichuraiana	Rw5G006180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	419
AccB1I	GGYRCC	4 cut(s)	636, 839, 977, 1699
AccB7I	CCANNNNNTGG	1 cut(s)	1279
AccBSI	CCGCTC	1 cut(s)	1551
AccI	GTMKAC	2 cut(s)	459, 1116
AccII	CGCG	3 cut(s)	290, 520, 1414
AclWI	GGATC	5 cut(s)	398, 683, 1025, 1079, 1092
AcoI	YGGCCR	3 cut(s)	579, 729, 1415
AcsI	RAATTY	1 cut(s)	101
AcyI	GRCGYC	1 cut(s)	978
AdeI	CACNNNGTG	1 cut(s)	1148
AfiI	CCNNNNNNNGG	8 cut(s)	143, 407, 581, 582, 974, 980, 1279, 1709
AgsI	TTSAA	3 cut(s)	106, 1510, 1738
AjiI	CACGTC	1 cut(s)	1279
AjnI	CCWGG	2 cut(s)	525, 1272
AluBI	AGCT	9 cut(s)	24, 397, 431, 490, 679, 817, 1064, 1721, 1747
AluI	AGCT	9 cut(s)	24, 397, 431, 490, 679, 817, 1064, 1721, 1747
Alw21I	GWGCWC	1 cut(s)	492
Alw26I	GTCTC	3 cut(s)	266, 954, 1385
AlwI	GGATC	5 cut(s)	398, 683, 1025, 1079, 1092
AoxI	GGCC	7 cut(s)	480, 498, 528, 537, 579, 729, 1415
ApeKI	GCWGC	2 cut(s)	431, 797
ApoI	RAATTY	1 cut(s)	101
AspLEI	GCGC	6 cut(s)	292, 420, 802, 956, 980, 1414
AspS9I	GGNCC	6 cut(s)	323, 410, 498, 529, 1023, 1219
AsuC2I	CCSGG	4 cut(s)	408, 583, 975, 1576
AsuHPI	GGTGA	9 cut(s)	85, 748, 877, 917, 953, 1094, 1483, 1558, 1702
AsuII	TTCGAA	1 cut(s)	1367
AsuNHI	GCTAGC	1 cut(s)	427
AvaII	GGWCC	4 cut(s)	323, 410, 1023, 1219
BaeGI	GKGCMC	1 cut(s)	1704
BamHI	GGATCC	1 cut(s)	1084
BanI	GGYRCC	4 cut(s)	636, 839, 977, 1699
BanII	GRGCYC	2 cut(s)	492, 948
BarI	GAAGNNNNNNTAC	2 cut(s)	638, 670
BauI	CACGAG	1 cut(s)	1143
BbsI	GAAGAC	1 cut(s)	1454
Bbv12I	GWGCWC	1 cut(s)	492
BbvCI	CCTCAGC	1 cut(s)	1351
BbvI	GCAGC	2 cut(s)	418, 809
BccI	CCATC	2 cut(s)	359, 1023
BceAI	ACGGC	5 cut(s)	265, 270, 1326, 1402, 1455
BcgI	CGANNNNNNTGC	4 cut(s)	664, 698, 1302, 1336
BciT130I	CCWGG	2 cut(s)	527, 1274
BclI	TGATCA	1 cut(s)	1473
BcnI	CCSGG	4 cut(s)	408, 583, 975, 1576
BcoDI	GTCTC	3 cut(s)	266, 954, 1385
BfaI	CTAG	7 cut(s)	311, 423, 428, 821, 867, 1244, 1259
BfmI	CTRYAG	1 cut(s)	949
BfoI	RGCGCY	2 cut(s)	957, 981
BglI	GCCNNNNNGGC	1 cut(s)	536
BisI	GCNGC	7 cut(s)	288, 432, 798, 1341, 1415, 1425, 1595
BlsI	GCNGC	7 cut(s)	289, 433, 799, 1342, 1416, 1426, 1596
Bme1390I	CCNGG	6 cut(s)	408, 527, 583, 975, 1274, 1576
Bme18I	GGWCC	4 cut(s)	323, 410, 1023, 1219
BmgBI	CACGTC	1 cut(s)	1279
BmgT120I	GGNCC	6 cut(s)	323, 410, 498, 529, 1023, 1219
BmiI	GGNNCC	6 cut(s)	411, 638, 841, 979, 1086, 1701
BmrFI	CCNGG	6 cut(s)	408, 527, 583, 975, 1274, 1576
BmrI	ACTGGG	2 cut(s)	449, 727
BmsI	GCATC	3 cut(s)	178, 811, 1525
BmtI	GCTAGC	1 cut(s)	431
BmuI	ACTGGG	2 cut(s)	449, 727
BpiI	GAAGAC	1 cut(s)	1454
BplI	GAGNNNNNCTC	2 cut(s)	867, 899
Bpu10I	CCTNAGC	2 cut(s)	138, 1351
Bpu14I	TTCGAA	1 cut(s)	1367
BpuEI	CTTGAG	1 cut(s)	1648
BpuMI	CCSGG	4 cut(s)	408, 583, 975, 1576
Bsa29I	ATCGAT	1 cut(s)	354
BsaAI	YACGTR	2 cut(s)	1235, 1525
BsaHI	GRCGYC	1 cut(s)	978
BsaI	GGTCTC	1 cut(s)	266
BsaJI	CCNNGG	5 cut(s)	540, 576, 632, 973, 1289
BsaWI	WCCGGW	2 cut(s)	789, 1025
BsaXI	ACNNNNNCTCC	8 cut(s)	731, 752, 761, 782, 1274, 1304, 1451, 1481
Bsc4I	CCNNNNNNNGG	8 cut(s)	143, 407, 581, 582, 974, 980, 1279, 1709
Bse118I	RCCGGY	1 cut(s)	731
Bse1I	ACTGG	2 cut(s)	455, 722
BseBI	CCWGG	2 cut(s)	527, 1274
BseCI	ATCGAT	1 cut(s)	354
BseDI	CCNNGG	5 cut(s)	540, 576, 632, 973, 1289
BseGI	GGATG	4 cut(s)	741, 862, 1011, 1546
BseLI	CCNNNNNNNGG	8 cut(s)	143, 407, 581, 582, 974, 980, 1279, 1709
BseMII	CTCAG	3 cut(s)	1052, 1086, 1365
BseNI	ACTGG	2 cut(s)	455, 722
BseRI	GAGGAG	4 cut(s)	760, 1472, 1475, 1685
BseSI	GKGCMC	1 cut(s)	1704
BseX3I	CGGCCG	2 cut(s)	729, 1415
BseXI	GCAGC	2 cut(s)	418, 809
BseYI	CCCAGC	2 cut(s)	20, 531
Bsh1236I	CGCG	3 cut(s)	290, 520, 1414
Bsh1285I	CGRYCG	2 cut(s)	732, 1418
BshFI	GGCC	7 cut(s)	482, 500, 530, 539, 581, 731, 1417
BshNI	GGYRCC	4 cut(s)	636, 839, 977, 1699
BshVI	ATCGAT	1 cut(s)	354
BsiEI	CGRYCG	2 cut(s)	732, 1418
BsiHKAI	GWGCWC	1 cut(s)	492
BslFI	GGGAC	2 cut(s)	734, 1165
BslI	CCNNNNNNNGG	8 cut(s)	143, 407, 581, 582, 974, 980, 1279, 1709
BsmAI	GTCTC	3 cut(s)	266, 954, 1385
BsmBI	CGTCTC	1 cut(s)	954
BsmFI	GGGAC	2 cut(s)	734, 1165
BsmI	GAATGC	1 cut(s)	281
BsnI	GGCC	7 cut(s)	482, 500, 530, 539, 581, 731, 1417
Bso31I	GGTCTC	1 cut(s)	266
Bsp119I	TTCGAA	1 cut(s)	1367
Bsp1286I	GDGCHC	3 cut(s)	492, 948, 1704
Bsp1407I	TGTACA	1 cut(s)	738
Bsp143I	GATC	9 cut(s)	403, 666, 688, 906, 1030, 1084, 1473, 1614, 1740
Bsp19I	CCATGG	2 cut(s)	540, 576
BspANI	GGCC	7 cut(s)	482, 500, 530, 539, 581, 731, 1417
BspCNI	CTCAG	3 cut(s)	1051, 1085, 1364
BspDI	ATCGAT	1 cut(s)	354
BspFNI	CGCG	3 cut(s)	290, 520, 1414
BspLI	GGNNCC	6 cut(s)	411, 638, 841, 979, 1086, 1701
BspOI	GCTAGC	1 cut(s)	431
BspPI	GGATC	5 cut(s)	398, 683, 1025, 1079, 1092
BspQI	GCTCTTC	2 cut(s)	629, 708
BspT104I	TTCGAA	1 cut(s)	1367
BspT107I	GGYRCC	4 cut(s)	636, 839, 977, 1699
BspTNI	GGTCTC	1 cut(s)	266
BsrBI	CCGCTC	1 cut(s)	1551
BsrFI	RCCGGY	1 cut(s)	731
BsrGI	TGTACA	1 cut(s)	738
BsrI	ACTGG	2 cut(s)	455, 722
BssAI	RCCGGY	1 cut(s)	731
BssECI	CCNNGG	5 cut(s)	540, 576, 632, 973, 1289
BssMI	GATC	9 cut(s)	403, 666, 688, 906, 1030, 1084, 1473, 1614, 1740
BssNAI	GTATAC	1 cut(s)	460
BssNI	GRCGYC	1 cut(s)	978
BssSI	CACGAG	1 cut(s)	1143
BssT1I	CCWWGG	3 cut(s)	540, 576, 632
Bst1107I	GTATAC	1 cut(s)	460
Bst2BI	CACGAG	1 cut(s)	1143
Bst2UI	CCWGG	2 cut(s)	527, 1274
Bst4CI	ACNGT	5 cut(s)	344, 551, 566, 641, 836
Bst6I	CTCTTC	3 cut(s)	629, 708, 938
BstACI	GRCGYC	1 cut(s)	978
BstAPI	GCANNNNNTGC	1 cut(s)	153
BstAUI	TGTACA	1 cut(s)	738
BstBAI	YACGTR	2 cut(s)	1235, 1525
BstBI	TTCGAA	1 cut(s)	1367
BstC8I	GCNNGC	8 cut(s)	238, 365, 429, 498, 537, 620, 677, 733
BstDEI	CTNAG	5 cut(s)	138, 1038, 1072, 1351, 1428
BstDSI	CCRYGG	3 cut(s)	540, 576, 1289
BstENI	CCTNNNNNAGG	1 cut(s)	141
BstF5I	GGATG	4 cut(s)	741, 862, 1011, 1546
BstFNI	CGCG	3 cut(s)	290, 520, 1414
BstH2I	RGCGCY	2 cut(s)	957, 981
BstHHI	GCGC	6 cut(s)	292, 420, 802, 956, 980, 1414
BstKTI	GATC	9 cut(s)	406, 669, 691, 909, 1033, 1087, 1476, 1617, 1743
BstMAI	GTCTC	3 cut(s)	266, 954, 1385
BstMBI	GATC	9 cut(s)	403, 666, 688, 906, 1030, 1084, 1473, 1614, 1740
BstMCI	CGRYCG	2 cut(s)	732, 1418
BstMWI	GCNNNNNNNGC	6 cut(s)	153, 287, 424, 536, 1571, 1594
BstNI	CCWGG	2 cut(s)	527, 1274
BstNSI	RCATGY	5 cut(s)	220, 321, 739, 1126, 1592
BstSCI	CCNGG	6 cut(s)	406, 525, 581, 973, 1272, 1574
BstSFI	CTRYAG	1 cut(s)	949
BstSLI	GKGCMC	1 cut(s)	1704
BstUI	CGCG	3 cut(s)	290, 520, 1414
BstV1I	GCAGC	2 cut(s)	418, 809
BstV2I	GAAGAC	1 cut(s)	1454
BstX2I	RGATCY	2 cut(s)	1030, 1084
BstYI	RGATCY	2 cut(s)	1030, 1084
BstZ17I	GTATAC	1 cut(s)	460
BstZI	CGGCCG	2 cut(s)	729, 1415
Bsu15I	ATCGAT	1 cut(s)	354
BsuRI	GGCC	7 cut(s)	482, 500, 530, 539, 581, 731, 1417
BsuTUI	ATCGAT	1 cut(s)	354
BtgI	CCRYGG	3 cut(s)	540, 576, 1289
BtrI	CACGTC	1 cut(s)	1279
BtsCI	GGATG	4 cut(s)	741, 862, 1011, 1546
BtsIMutI	CAGTG	3 cut(s)	56, 547, 1483
Cac8I	GCNNGC	8 cut(s)	238, 365, 429, 498, 537, 620, 677, 733
CfoI	GCGC	6 cut(s)	292, 420, 802, 956, 980, 1414
Cfr10I	RCCGGY	1 cut(s)	731
Cfr13I	GGNCC	6 cut(s)	323, 410, 498, 529, 1023, 1219
ClaI	ATCGAT	1 cut(s)	354
CpoI	CGGWCCG	1 cut(s)	323
CseI	GACGC	1 cut(s)	731
CspCI	CAANNNNNGTGG	2 cut(s)	817, 852
CspI	CGGWCCG	1 cut(s)	323
DdeI	CTNAG	5 cut(s)	138, 1038, 1072, 1351, 1428
DinI	GGCGCC	1 cut(s)	979
DpnI	GATC	9 cut(s)	405, 668, 690, 908, 1032, 1086, 1475, 1616, 1742
DpnII	GATC	9 cut(s)	403, 666, 688, 906, 1030, 1084, 1473, 1614, 1740
DraIII	CACNNNGTG	1 cut(s)	1148
EaeI	YGGCCR	3 cut(s)	579, 729, 1415
EagI	CGGCCG	2 cut(s)	729, 1415
Eam1104I	CTCTTC	3 cut(s)	629, 708, 938
EarI	CTCTTC	3 cut(s)	629, 708, 938
EciI	GGCGGA	4 cut(s)	344, 1457, 1580, 1682
Ecl136II	GAGCTC	1 cut(s)	490
EclXI	CGGCCG	2 cut(s)	729, 1415
Eco130I	CCWWGG	3 cut(s)	540, 576, 632
Eco24I	GRGCYC	2 cut(s)	492, 948
Eco31I	GGTCTC	1 cut(s)	266
Eco47I	GGWCC	4 cut(s)	323, 410, 1023, 1219
Eco52I	CGGCCG	2 cut(s)	729, 1415
Eco53kI	GAGCTC	1 cut(s)	490
EcoICRI	GAGCTC	1 cut(s)	490
EcoNI	CCTNNNNNAGG	1 cut(s)	141
EcoO109I	RGGNCCY	1 cut(s)	410
EcoRII	CCWGG	2 cut(s)	525, 1272
EcoT14I	CCWWGG	3 cut(s)	540, 576, 632
EcoT38I	GRGCYC	2 cut(s)	492, 948
EgeI	GGCGCC	1 cut(s)	979
EheI	GGCGCC	1 cut(s)	979
ErhI	CCWWGG	3 cut(s)	540, 576, 632
Esp3I	CGTCTC	1 cut(s)	954
FalI	AAGNNNNNCTT	2 cut(s)	1610, 1642
FaqI	GGGAC	2 cut(s)	734, 1165
FauI	CCCGC	5 cut(s)	250, 372, 489, 525, 1544
FbaI	TGATCA	1 cut(s)	1473
FblI	GTMKAC	2 cut(s)	459, 1116
Fnu4HI	GCNGC	7 cut(s)	288, 432, 798, 1341, 1415, 1425, 1595
FokI	GGATG	4 cut(s)	728, 869, 998, 1553
FriOI	GRGCYC	2 cut(s)	492, 948
Fsp4HI	GCNGC	7 cut(s)	288, 432, 798, 1341, 1415, 1425, 1595
FspAI	RTGCGCAY	1 cut(s)	419
FspBI	CTAG	7 cut(s)	311, 423, 428, 821, 867, 1244, 1259
FspI	TGCGCA	1 cut(s)	419
GlaI	GCGC	6 cut(s)	291, 419, 801, 955, 979, 1413
GluI	GCNGC	7 cut(s)	288, 432, 798, 1341, 1415, 1425, 1595
GsaI	CCCAGC	2 cut(s)	24, 535
HaeII	RGCGCY	2 cut(s)	957, 981
HaeIII	GGCC	7 cut(s)	482, 500, 530, 539, 581, 731, 1417
HgaI	GACGC	1 cut(s)	731
HhaI	GCGC	6 cut(s)	292, 420, 802, 956, 980, 1414
Hin1I	GRCGYC	1 cut(s)	978
Hin6I	GCGC	6 cut(s)	290, 418, 800, 954, 978, 1412
HinP1I	GCGC	6 cut(s)	290, 418, 800, 954, 978, 1412
HindIII	AAGCTT	1 cut(s)	815
HinfI	GANTC	6 cut(s)	870, 1213, 1238, 1250, 1285, 1645
HphI	GGTGA	9 cut(s)	85, 748, 877, 917, 953, 1094, 1483, 1558, 1702
Hpy166II	GTNNAC	5 cut(s)	460, 547, 610, 1117, 1522
Hpy188I	TCNGA	5 cut(s)	592, 813, 875, 1041, 1223
Hpy188III	TCNNGA	7 cut(s)	263, 712, 821, 991, 1021, 1618, 1738
Hpy8I	GTNNAC	5 cut(s)	460, 547, 610, 1117, 1522
Hpy99I	CGWCG	6 cut(s)	446, 515, 566, 689, 1384, 1423
HpyAV	CCTTC	6 cut(s)	97, 129, 472, 718, 1447, 1549
HpyCH4III	ACNGT	5 cut(s)	344, 551, 566, 641, 836
HpyCH4IV	ACGT	6 cut(s)	441, 1113, 1234, 1278, 1382, 1524
HpyCH4V	TGCA	4 cut(s)	156, 281, 675, 1588
HpyF10VI	GCNNNNNNNGC	6 cut(s)	153, 287, 424, 536, 1571, 1594
HpyF3I	CTNAG	5 cut(s)	138, 1038, 1072, 1351, 1428
HpySE526I	ACGT	6 cut(s)	441, 1113, 1234, 1278, 1382, 1524
Hsp92I	GRCGYC	1 cut(s)	978
HspAI	GCGC	6 cut(s)	290, 418, 800, 954, 978, 1412
KasI	GGCGCC	1 cut(s)	977
KroI	GCCGGC	1 cut(s)	731
KroNI	GCCGGC	1 cut(s)	733
Ksp22I	TGATCA	1 cut(s)	1473
Kzo9I	GATC	9 cut(s)	403, 666, 688, 906, 1030, 1084, 1473, 1614, 1740
LguI	GCTCTTC	2 cut(s)	629, 708
LmnI	GCTCC	3 cut(s)	730, 1061, 1718
Lsp1109I	GCAGC	2 cut(s)	418, 809
LweI	GCATC	3 cut(s)	178, 811, 1525
MaeI	CTAG	7 cut(s)	311, 423, 428, 821, 867, 1244, 1259
MaeII	ACGT	6 cut(s)	441, 1113, 1234, 1278, 1382, 1524
MaeIII	GTNAC	3 cut(s)	79, 1235, 1690
MalI	GATC	9 cut(s)	405, 668, 690, 908, 1032, 1086, 1475, 1616, 1742
MbiI	CCGCTC	1 cut(s)	1551
MboI	GATC	9 cut(s)	403, 666, 688, 906, 1030, 1084, 1473, 1614, 1740
MflI	RGATCY	2 cut(s)	1030, 1084
MhlI	GDGCHC	3 cut(s)	492, 948, 1704
MluCI	AATT	2 cut(s)	101, 200
Mly113I	GGCGCC	1 cut(s)	978
MlyI	GAGTC	2 cut(s)	1232, 1259
MmeI	TCCRAC	2 cut(s)	615, 1058
MroNI	GCCGGC	1 cut(s)	731
MseI	TTAA	2 cut(s)	393, 1227
MslI	CAYNNNNRTG	1 cut(s)	890
MspA1I	CMGCKG	2 cut(s)	24, 1355
MspR9I	CCNGG	6 cut(s)	408, 527, 583, 975, 1274, 1576
Mva1269I	GAATGC	1 cut(s)	281
MvaI	CCWGG	2 cut(s)	527, 1274
MvnI	CGCG	3 cut(s)	290, 520, 1414
MwoI	GCNNNNNNNGC	6 cut(s)	153, 287, 424, 536, 1571, 1594
NaeI	GCCGGC	1 cut(s)	733
NarI	GGCGCC	1 cut(s)	978
NciI	CCSGG	4 cut(s)	408, 583, 975, 1576
NcoI	CCATGG	2 cut(s)	540, 576
NdeII	GATC	9 cut(s)	403, 666, 688, 906, 1030, 1084, 1473, 1614, 1740
NgoMIV	GCCGGC	1 cut(s)	731
NheI	GCTAGC	1 cut(s)	427
NlaIV	GGNNCC	6 cut(s)	411, 638, 841, 979, 1086, 1701
NmuCI	GTSAC	3 cut(s)	79, 1235, 1690
NsbI	TGCGCA	1 cut(s)	419
NspI	RCATGY	5 cut(s)	220, 321, 739, 1126, 1592
NspV	TTCGAA	1 cut(s)	1367
PciSI	GCTCTTC	2 cut(s)	629, 708
PcsI	WCGNNNNNNNCGW	3 cut(s)	291, 840, 1497
PctI	GAATGC	1 cut(s)	281
PdiI	GCCGGC	1 cut(s)	733
PfeI	GAWTC	4 cut(s)	870, 1213, 1285, 1645
PflMI	CCANNNNNTGG	1 cut(s)	1279
PkrI	GCNGC	7 cut(s)	289, 433, 799, 1342, 1416, 1426, 1596
PleI	GAGTC	2 cut(s)	1232, 1258
PluTI	GGCGCC	1 cut(s)	981
PpsI	GAGTC	2 cut(s)	1232, 1258
Ppu21I	YACGTR	2 cut(s)	1235, 1525
PpuMI	RGGWCCY	1 cut(s)	410
Psp124BI	GAGCTC	1 cut(s)	492
Psp5II	RGGWCCY	1 cut(s)	410
Psp6I	CCWGG	2 cut(s)	525, 1272
PspFI	CCCAGC	2 cut(s)	20, 531
PspGI	CCWGG	2 cut(s)	525, 1272
PspN4I	GGNNCC	6 cut(s)	411, 638, 841, 979, 1086, 1701
PspPI	GGNCC	6 cut(s)	323, 410, 498, 529, 1023, 1219
PspPPI	RGGWCCY	1 cut(s)	410
PsuI	RGATCY	2 cut(s)	1030, 1084
PvuII	CAGCTG	1 cut(s)	24
RseI	CAYNNNNRTG	1 cut(s)	890
Rsr2I	CGGWCCG	1 cut(s)	323
RsrII	CGGWCCG	1 cut(s)	323
SacI	GAGCTC	1 cut(s)	492
SapI	GCTCTTC	2 cut(s)	629, 708
SaqAI	TTAA	2 cut(s)	393, 1227
SatI	GCNGC	7 cut(s)	288, 432, 798, 1341, 1415, 1425, 1595
Sau3AI	GATC	9 cut(s)	403, 666, 688, 906, 1030, 1084, 1473, 1614, 1740
Sau96I	GGNCC	6 cut(s)	323, 410, 498, 529, 1023, 1219
SchI	GAGTC	2 cut(s)	1232, 1259
ScrFI	CCNGG	6 cut(s)	408, 527, 583, 975, 1274, 1576
SduI	GDGCHC	3 cut(s)	492, 948, 1704
SfaNI	GCATC	3 cut(s)	178, 811, 1525
SfcI	CTRYAG	1 cut(s)	949
SfiI	GGCCNNNNNGGCC	1 cut(s)	536
SfoI	GGCGCC	1 cut(s)	979
SfuI	TTCGAA	1 cut(s)	1367
SinI	GGWCC	4 cut(s)	323, 410, 1023, 1219
SmiMI	CAYNNNNRTG	1 cut(s)	890
SmlI	CTYRAG	1 cut(s)	1627
SmoI	CTYRAG	1 cut(s)	1627
Sse9I	AATT	2 cut(s)	101, 200
SspDI	GGCGCC	1 cut(s)	977
SspMI	CTAG	7 cut(s)	311, 423, 428, 821, 867, 1244, 1259
SstI	GAGCTC	1 cut(s)	492
StyD4I	CCNGG	6 cut(s)	406, 525, 581, 973, 1272, 1574
StyI	CCWWGG	3 cut(s)	540, 576, 632
TaaI	ACNGT	5 cut(s)	344, 551, 566, 641, 836
TaiI	ACGT	6 cut(s)	444, 1116, 1237, 1281, 1385, 1527
TasI	AATT	2 cut(s)	101, 200
TatI	WGTACW	2 cut(s)	26, 738
TauI	GCSGC	5 cut(s)	290, 1343, 1417, 1427, 1597
TfiI	GAWTC	4 cut(s)	870, 1213, 1285, 1645
Tru1I	TTAA	2 cut(s)	393, 1227
Tru9I	TTAA	2 cut(s)	393, 1227
TscAI	CASTG	3 cut(s)	63, 554, 1483
TseFI	GTSAC	3 cut(s)	79, 1235, 1690
TseI	GCWGC	2 cut(s)	431, 797
Tsp45I	GTSAC	3 cut(s)	79, 1235, 1690
TspDTI	ATGAA	1 cut(s)	111
TspGWI	ACGGA	5 cut(s)	264, 869, 1062, 1278, 1670
TspRI	CASTG	3 cut(s)	63, 554, 1483
Van91I	CCANNNNNTGG	1 cut(s)	1279
VpaK11BI	GGWCC	4 cut(s)	323, 410, 1023, 1219
XagI	CCTNNNNNAGG	1 cut(s)	141
XapI	RAATTY	1 cut(s)	101
XbaI	TCTAGA	1 cut(s)	820
XceI	RCATGY	5 cut(s)	220, 321, 739, 1126, 1592
XmiI	GTMKAC	2 cut(s)	459, 1116
XspI	CTAG	7 cut(s)	311, 423, 428, 821, 867, 1244, 1259

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.