Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006919

Physical Location & Seq

Genomic Coordinates Forward (+)

44759206 .. 44760969

Gene Structure 1764 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1320100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGCTTCATCTCCTTTACCATCAACTTCTACAATGGCCGCCGTGCACTCCACCACCACAACCACCCCCTTCCGCTCTCCTTTATTCCCAAACAAGTACCAGACTTCATTGCAACGAAAACCCAAACAATGCCTTGCAGGCAGAGTGCGCTGTAAAGCAACAAAAGGTGACAATGAAAACCTAGACCAGGGCCTAGCAAGACTCGACAGGAGGAACATGCTGATAGGTTTAGGCGCTGGGGGTCTCTACGGTGCAGCAGGAAACCCATTTGCTTTTGCAGCACCGGTATCCGCCCCAGATCTGACCACGTGTGGTCCTGCCGACAAGCCAGACGGGTCCACCATCGATTGTTGCCCACCCACCACGACGACCATCATCGACTTCAAACTCCCTGACCGAGGCCCACTCCGCACTAGGCTCGCTGCCCAGGACGTTGCAAAAAACCCTGCATACTTGGCCAAATACAAAAAGGCCATCGAGCTGATGCGGGCACTTCCAGACGACGACCCGCGCAGTTTCGCCCAACAGGCCAAAGTCCACTGCTCTTACTGCGATGGTGGATACCCACAAGTCGGATACTCGGATTTGGAGATCCAAGTTCACTACTGTTGGCTCTTCTTCCCGTTCCATCGTTGGTACCTCTACTTCTACGAGAAAATCATGGGGGAGCTCATTGGGGACCCAACCTTCGCCCTCCCCTTCTGGAACTGGGACGCACCAGCTGGAATGTACATTCCTGAGATTTTCACTGATACGTCGTCATCCCTCTACGACCAGTATAGAAATGCAGCGCATCAGCCCCCGAAGCTCTTAGACTTTAATTACAGCGGGACCGACGATAACGTCGACGACGCAACACGAATCAAAGAGAACTTAACAACAATGTACCAGCAGATGGTGTCAAAGGCCACTTCTCACAGACTCTTTTTTGGAGAGCCCTACAGCGCAGGGGACGACCCAAGTCCTGGTGCCGGAAACATTGAGAGCATTCCCCATAACAATATTCACTTTTGGACTGGCGACCCAACCCAGACAAATGGGGAGGACATGGGGGCCTTTTACTCTTCGGGGAGGGATCCGCTGTTTTACTCCCACCATTCCAACGTCGACCGCATGTGGTCTATATATAAAGATAAGTTGGGAGGTACGGACATAGAAAAGACTGACTGGTTGGACACGGAGTTTTTGTTCTACGACGAGAAAAAGAATCTTGTGCGCGTCAAGGTTTGTGACTCGCTCGACACGAAAAAACTCGGGTACGTGTACGATGAGAAAGTCCCGATCCCGTGGCTGAAATCGAAGCCGACAACTCGTAAGTCGACGAATAAGAGAAAGGCCGCAGTTTCATCTTCTGATCTTACTACAACGTTCCCTGCTACACTGTCGGATACAATCAGCGTCGAGGTGACGAGGCCATCTGCGACGAAGCGGACAACTGCCCAGAAGAAGGCGCAGGACGAGGTGCTGGTGATTAAGGGGATTGAGTTTGCCGGGAATGAGACTGTGAAGTTCGACGTGTATGTGAACGATGACGCGGACTCACTGGCCGGGAAAGACAAGTCGGAGTTTGCTGGGAGTTTTGTTCACGTGCCGCATAAGCATAAGAAAAATATTAAGACGAACCTGCGGCTGAGCATTACAAGCTTGTTGGAGGAGTTGGATGCGGAGACAGACAGCAGTTTAGTGGTGACTTTGGTGCCAAAAGTTGGGAAGGGGCCAATCACCATCGGAGGGTTTAGCATTGAGCTCATTAATACTACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

588

Amino Acids

65.17

Weight (kDa)

6.38

Isoelectric Point (pI)

40.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	171 - 378	2.3e-36	Common central domain of tyrosinase
PPO1_DWL	PF12142	384 - 436	2.6e-23	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	457 - 583	9.6e-48	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g04680
malus_domestica	MD05G1319800.v1.1 MD05G1320100.v1.1 MD05G1320200.v1.1 MD05G1320500.v1.1 MD05G1320800.v1.1 MD10G1298300.v1.1
prunus_persica	Prupe.4G041500_v2.0.a1 Prupe.4G041700_v2.0.a1
pyrus_communis	pycom05g29530 pycom05g29560 pycom10g24890 pycom10g24910 pycom10g25140
rosa_chinensis	RchiOBHm_Chr5g0007621
rosa_laevigata	RLG00000031479
rosa_multiflora	Rmu_co8439317.1_g000001 Rmu_sc0011744.1_g000006 Rmu_sc0021005.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068730
rosa_rugosa	Rorug04G0429400
rosa_samantha	Rh5AG057500 Rh5BG054400 Rh5CG064400 Rh5DG053600
rosa_wichuraiana	Rw5G006180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	842
Acc36I	ACCTGC	1 cut(s)	1632
Acc65I	GGTACC	1 cut(s)	636
AccB1I	GGYRCC	3 cut(s)	636, 968, 1696
AccB7I	CCANNNNNTGG	3 cut(s)	895, 965, 1706
AccBSI	CCGCTC	1 cut(s)	75
AccI	GTMKAC	3 cut(s)	846, 1107, 1319
AccII	CGCG	3 cut(s)	511, 1218, 1535
AclI	AACGTT	1 cut(s)	1367
AclWI	GGATC	4 cut(s)	586, 1070, 1083, 1276
AcoI	YGGCCR	3 cut(s)	36, 456, 1545
AcvI	CACGTG	2 cut(s)	309, 1588
AfaI	GTAC	7 cut(s)	98, 638, 731, 887, 1147, 1259, 1265
AfiI	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
AflIII	ACRYGT	3 cut(s)	308, 1260, 1515
AgeI	ACCGGT	1 cut(s)	283
AgsI	TTSAA	1 cut(s)	385
AjiI	CACGTC	1 cut(s)	1516
AjnI	CCWGG	3 cut(s)	186, 426, 964
AloI	GAACNNNNNNTCC	2 cut(s)	1172, 1204
AluBI	AGCT	6 cut(s)	481, 670, 722, 808, 1644, 1747
AluI	AGCT	6 cut(s)	481, 670, 722, 808, 1644, 1747
Alw21I	GWGCWC	3 cut(s)	48, 672, 1749
Alw26I	GTCTC	3 cut(s)	248, 1493, 1661
Alw44I	GTGCAC	1 cut(s)	44
AlwI	GGATC	4 cut(s)	586, 1070, 1083, 1276
Ama87I	CYCGRG	1 cut(s)	1253
ApaLI	GTGCAC	1 cut(s)	44
ApeKI	GCWGC	4 cut(s)	254, 278, 422, 788
ArsI	GACNNNNNNTTYG	2 cut(s)	671, 703
AseI	ATTAAT	1 cut(s)	1752
AsiGI	ACCGGT	1 cut(s)	283
Asp718I	GGTACC	1 cut(s)	636
AspLEI	GCGC	7 cut(s)	150, 236, 513, 793, 947, 1218, 1453
AspS9I	GGNCC	8 cut(s)	190, 314, 336, 401, 679, 831, 1053, 1715
AsuC2I	CCSGG	2 cut(s)	1492, 1549
AsuHPI	GGTGA	5 cut(s)	179, 1417, 1480, 1699, 1714
AvaI	CYCGRG	1 cut(s)	1253
AvaII	GGWCC	4 cut(s)	314, 336, 679, 831
BaeGI	GKGCMC	2 cut(s)	48, 493
BalI	TGGCCA	1 cut(s)	458
BamHI	GGATCC	1 cut(s)	1075
BanI	GGYRCC	3 cut(s)	636, 968, 1696
BanII	GRGCYC	3 cut(s)	672, 939, 1749
BarI	GAAGNNNNNNTAC	2 cut(s)	629, 661
BbrPI	CACGTG	2 cut(s)	309, 1588
Bbv12I	GWGCWC	3 cut(s)	48, 672, 1749
BbvI	GCAGC	4 cut(s)	266, 290, 409, 800
BccI	CCATC	9 cut(s)	28, 350, 380, 482, 548, 636, 889, 1423, 1733
BceAI	ACGGC	1 cut(s)	26
BcgI	CGANNNNNNTGC	2 cut(s)	400, 434
BciT130I	CCWGG	3 cut(s)	188, 428, 966
BciVI	GTATCC	4 cut(s)	298, 554, 569, 1381
BcnI	CCSGG	2 cut(s)	1492, 1549
BcoDI	GTCTC	3 cut(s)	248, 1493, 1661
BfaI	CTAG	3 cut(s)	182, 194, 414
BfmI	CTRYAG	1 cut(s)	940
BfoI	RGCGCY	1 cut(s)	237
BfuAI	ACCTGC	1 cut(s)	1632
BfuI	GTATCC	4 cut(s)	298, 554, 569, 1381
BglI	GCCNNNNNGGC	2 cut(s)	138, 527
BglII	AGATCT	1 cut(s)	298
BisI	GCNGC	8 cut(s)	39, 255, 279, 423, 789, 1338, 1592, 1628
BlpI	GCTNAGC	1 cut(s)	1631
BlsI	GCNGC	8 cut(s)	40, 256, 280, 424, 790, 1339, 1593, 1629
Bme1390I	CCNGG	5 cut(s)	188, 428, 966, 1492, 1549
Bme18I	GGWCC	4 cut(s)	314, 336, 679, 831
BmeT110I	CYCGRG	1 cut(s)	1253
BmgBI	CACGTC	1 cut(s)	1516
BmgT120I	GGNCC	8 cut(s)	190, 314, 336, 401, 679, 831, 1053, 1715
BmrFI	CCNGG	5 cut(s)	188, 428, 966, 1492, 1549
BmrI	ACTGGG	1 cut(s)	718
BmsI	GCATC	3 cut(s)	474, 802, 1651
BmuI	ACTGGG	1 cut(s)	718
BoxI	GACNNNNGTC	1 cut(s)	960
BplI	GAGNNNNNCTC	2 cut(s)	390, 422
Bpu1102I	GCTNAGC	1 cut(s)	1631
BpuMI	CCSGG	2 cut(s)	1492, 1549
Bsa29I	ATCGAT	1 cut(s)	345
BsaAI	YACGTR	3 cut(s)	309, 1261, 1588
BsaI	GGTCTC	1 cut(s)	248
BsaJI	CCNNGG	4 cut(s)	187, 397, 426, 1286
BsaWI	WCCGGW	1 cut(s)	283
BsaXI	ACNNNNNCTCC	2 cut(s)	1172, 1202
Bsc4I	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
Bse118I	RCCGGY	1 cut(s)	283
Bse1I	ACTGG	5 cut(s)	713, 775, 1021, 1172, 1548
Bse3DI	GCAATG	1 cut(s)	107
BseBI	CCWGG	3 cut(s)	188, 428, 966
BseCI	ATCGAT	1 cut(s)	345
BseDI	CCNNGG	4 cut(s)	187, 397, 426, 1286
BseGI	GGATG	2 cut(s)	761, 1666
BseLI	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
BseMI	GCAATG	1 cut(s)	107
BseMII	CTCAG	2 cut(s)	729, 1622
BseNI	ACTGG	5 cut(s)	713, 775, 1021, 1172, 1548
BseRI	GAGGAG	1 cut(s)	1667
BseSI	GKGCMC	2 cut(s)	48, 493
BseXI	GCAGC	4 cut(s)	266, 290, 409, 800
BseYI	CCCAGC	2 cut(s)	236, 1571
BsgI	GTGCAG	1 cut(s)	273
Bsh1236I	CGCG	3 cut(s)	511, 1218, 1535
Bsh1285I	CGRYCG	1 cut(s)	1111
BshNI	GGYRCC	3 cut(s)	636, 968, 1696
BshTI	ACCGGT	1 cut(s)	283
BshVI	ATCGAT	1 cut(s)	345
BsiEI	CGRYCG	1 cut(s)	1111
BsiHKAI	GWGCWC	3 cut(s)	48, 672, 1749
BsiHKCI	CYCGRG	1 cut(s)	1253
BsiSI	CCGG	4 cut(s)	284, 972, 1491, 1548
BslFI	GGGAC	5 cut(s)	692, 725, 844, 965, 1262
BslI	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
BsmAI	GTCTC	3 cut(s)	248, 1493, 1661
BsmFI	GGGAC	5 cut(s)	692, 725, 844, 965, 1262
BsmI	GAATGC	1 cut(s)	987
Bso31I	GGTCTC	1 cut(s)	248
BsoBI	CYCGRG	1 cut(s)	1253
Bsp1286I	GDGCHC	5 cut(s)	48, 493, 672, 939, 1749
Bsp1407I	TGTACA	1 cut(s)	729
Bsp143I	GATC	5 cut(s)	298, 591, 1075, 1281, 1354
Bsp1720I	GCTNAGC	1 cut(s)	1631
BspCNI	CTCAG	2 cut(s)	730, 1623
BspDI	ATCGAT	1 cut(s)	345
BspFNI	CGCG	3 cut(s)	511, 1218, 1535
BspMI	ACCTGC	1 cut(s)	1632
BspPI	GGATC	4 cut(s)	586, 1070, 1083, 1276
BspQI	GCTCTTC	1 cut(s)	620
BspT107I	GGYRCC	3 cut(s)	636, 968, 1696
BspTNI	GGTCTC	1 cut(s)	248
BsrBI	CCGCTC	1 cut(s)	75
BsrDI	GCAATG	1 cut(s)	107
BsrFI	RCCGGY	1 cut(s)	283
BsrGI	TGTACA	1 cut(s)	729
BsrI	ACTGG	5 cut(s)	713, 775, 1021, 1172, 1548
BssAI	RCCGGY	1 cut(s)	283
BssECI	CCNNGG	4 cut(s)	187, 397, 426, 1286
BssMI	GATC	5 cut(s)	298, 591, 1075, 1281, 1354
Bst2UI	CCWGG	3 cut(s)	188, 428, 966
Bst4CI	ACNGT	4 cut(s)	251, 608, 1383, 1504
Bst6I	CTCTTC	2 cut(s)	620, 1069
BstAUI	TGTACA	1 cut(s)	729
BstBAI	YACGTR	3 cut(s)	309, 1261, 1588
BstC8I	GCNNGC	3 cut(s)	139, 420, 489
BstDEI	CTNAG	3 cut(s)	738, 811, 1631
BstDSI	CCRYGG	1 cut(s)	1286
BstENI	CCTNNNNNAGG	2 cut(s)	185, 396
BstF5I	GGATG	2 cut(s)	761, 1666
BstFNI	CGCG	3 cut(s)	511, 1218, 1535
BstH2I	RGCGCY	1 cut(s)	237
BstHHI	GCGC	7 cut(s)	150, 236, 513, 793, 947, 1218, 1453
BstKTI	GATC	5 cut(s)	301, 594, 1078, 1284, 1357
BstMAI	GTCTC	3 cut(s)	248, 1493, 1661
BstMBI	GATC	5 cut(s)	298, 591, 1075, 1281, 1354
BstMCI	CGRYCG	1 cut(s)	1111
BstNI	CCWGG	3 cut(s)	188, 428, 966
BstNSI	RCATGY	2 cut(s)	220, 1117
BstPAI	GACNNNNGTC	1 cut(s)	960
BstSCI	CCNGG	5 cut(s)	186, 426, 964, 1490, 1547
BstSFI	CTRYAG	1 cut(s)	940
BstSLI	GKGCMC	2 cut(s)	48, 493
BstUI	CGCG	3 cut(s)	511, 1218, 1535
BstV1I	GCAGC	4 cut(s)	266, 290, 409, 800
BstX2I	RGATCY	3 cut(s)	298, 591, 1075
BstXI	CCANNNNNNTGG	1 cut(s)	1037
BstYI	RGATCY	3 cut(s)	298, 591, 1075
Bsu15I	ATCGAT	1 cut(s)	345
BsuI	GTATCC	4 cut(s)	298, 554, 569, 1381
BsuTUI	ATCGAT	1 cut(s)	345
BtgI	CCRYGG	1 cut(s)	1286
BtgZI	GCGATG	1 cut(s)	567
BtrI	CACGTC	1 cut(s)	1516
BtsCI	GGATG	2 cut(s)	761, 1666
BtsI	GCAGTG	1 cut(s)	538
BtsIMutI	CAGTG	4 cut(s)	538, 747, 1379, 1541
BveI	ACCTGC	1 cut(s)	1632
Cac8I	GCNNGC	3 cut(s)	139, 420, 489
CfoI	GCGC	7 cut(s)	150, 236, 513, 793, 947, 1218, 1453
Cfr10I	RCCGGY	1 cut(s)	283
Cfr13I	GGNCC	8 cut(s)	190, 314, 336, 401, 679, 831, 1053, 1715
ClaI	ATCGAT	1 cut(s)	345
CseI	GACGC	5 cut(s)	722, 860, 1207, 1387, 1541
Csp6I	GTAC	7 cut(s)	97, 637, 730, 886, 1146, 1258, 1264
CspAI	ACCGGT	1 cut(s)	283
CviAII	CATG	4 cut(s)	217, 661, 1048, 1114
CviQI	GTAC	7 cut(s)	97, 637, 730, 886, 1146, 1258, 1264
DdeI	CTNAG	3 cut(s)	738, 811, 1631
DpnI	GATC	5 cut(s)	300, 593, 1077, 1283, 1356
DpnII	GATC	5 cut(s)	298, 591, 1075, 1281, 1354
DrdI	GACNNNNNNGTC	1 cut(s)	842
DseDI	GACNNNNNNGTC	1 cut(s)	842
EaeI	YGGCCR	3 cut(s)	36, 456, 1545
Eam1104I	CTCTTC	2 cut(s)	620, 1069
EarI	CTCTTC	2 cut(s)	620, 1069
EciI	GGCGGA	1 cut(s)	280
Ecl136II	GAGCTC	2 cut(s)	670, 1747
Eco24I	GRGCYC	3 cut(s)	672, 939, 1749
Eco31I	GGTCTC	1 cut(s)	248
Eco47I	GGWCC	4 cut(s)	314, 336, 679, 831
Eco53kI	GAGCTC	2 cut(s)	670, 1747
Eco72I	CACGTG	2 cut(s)	309, 1588
Eco88I	CYCGRG	1 cut(s)	1253
EcoICRI	GAGCTC	2 cut(s)	670, 1747
EcoNI	CCTNNNNNAGG	2 cut(s)	185, 396
EcoO109I	RGGNCCY	3 cut(s)	190, 679, 1053
EcoRII	CCWGG	3 cut(s)	186, 426, 964
EcoT38I	GRGCYC	3 cut(s)	672, 939, 1749
FaeI	CATG	4 cut(s)	220, 664, 1051, 1117
FaqI	GGGAC	5 cut(s)	692, 725, 844, 965, 1262
FatI	CATG	4 cut(s)	216, 660, 1047, 1113
FauI	CCCGC	3 cut(s)	480, 516, 821
FblI	GTMKAC	3 cut(s)	846, 1107, 1319
Fnu4HI	GCNGC	8 cut(s)	39, 255, 279, 423, 789, 1338, 1592, 1628
FokI	GGATG	2 cut(s)	748, 1673
FriOI	GRGCYC	3 cut(s)	672, 939, 1749
Fsp4HI	GCNGC	8 cut(s)	39, 255, 279, 423, 789, 1338, 1592, 1628
FspBI	CTAG	3 cut(s)	182, 194, 414
GlaI	GCGC	7 cut(s)	149, 235, 512, 792, 946, 1217, 1452
GluI	GCNGC	8 cut(s)	39, 255, 279, 423, 789, 1338, 1592, 1628
GsaI	CCCAGC	2 cut(s)	240, 1575
HaeII	RGCGCY	1 cut(s)	237
HapII	CCGG	4 cut(s)	284, 972, 1491, 1548
HgaI	GACGC	5 cut(s)	722, 860, 1207, 1387, 1541
HhaI	GCGC	7 cut(s)	150, 236, 513, 793, 947, 1218, 1453
Hin1II	CATG	4 cut(s)	220, 664, 1051, 1117
Hin6I	GCGC	7 cut(s)	148, 234, 511, 791, 945, 1216, 1451
HinP1I	GCGC	7 cut(s)	148, 234, 511, 791, 945, 1216, 1451
HincII	GTYRAC	3 cut(s)	847, 1108, 1320
HindII	GTYRAC	3 cut(s)	847, 1108, 1320
HindIII	AAGCTT	1 cut(s)	1642
HinfI	GANTC	6 cut(s)	201, 861, 921, 1207, 1232, 1538
HpaII	CCGG	4 cut(s)	284, 972, 1491, 1548
HphI	GGTGA	5 cut(s)	179, 1417, 1480, 1699, 1714
Hpy188I	TCNGA	7 cut(s)	303, 575, 583, 1354, 1387, 1564, 1730
Hpy188III	TCNNGA	4 cut(s)	497, 703, 737, 1279
HpyAV	CCTTC	5 cut(s)	79, 697, 709, 1441, 1705
HpyCH4III	ACNGT	4 cut(s)	251, 608, 1383, 1504
HpyCH4IV	ACGT	9 cut(s)	308, 432, 755, 843, 1104, 1260, 1367, 1515, 1587
HpyCH4V	TGCA	8 cut(s)	46, 112, 137, 254, 278, 437, 449, 788
HpyF3I	CTNAG	3 cut(s)	738, 811, 1631
HpySE526I	ACGT	9 cut(s)	308, 432, 755, 843, 1104, 1260, 1367, 1515, 1587
Hsp92II	CATG	4 cut(s)	220, 664, 1051, 1117
HspAI	GCGC	7 cut(s)	148, 234, 511, 791, 945, 1216, 1451
KflI	GGGWCCC	1 cut(s)	679
KpnI	GGTACC	1 cut(s)	640
Kzo9I	GATC	5 cut(s)	298, 591, 1075, 1281, 1354
LguI	GCTCTTC	1 cut(s)	620
LmnI	GCTCC	1 cut(s)	667
Lsp1109I	GCAGC	4 cut(s)	266, 290, 409, 800
LweI	GCATC	3 cut(s)	474, 802, 1651
MaeI	CTAG	3 cut(s)	182, 194, 414
MaeII	ACGT	9 cut(s)	308, 432, 755, 843, 1104, 1260, 1367, 1515, 1587
MaeIII	GTNAC	4 cut(s)	167, 1229, 1405, 1687
MalI	GATC	5 cut(s)	300, 593, 1077, 1283, 1356
MbiI	CCGCTC	1 cut(s)	75
MboI	GATC	5 cut(s)	298, 591, 1075, 1281, 1354
MboII	GAAGA	5 cut(s)	607, 610, 1056, 1341, 1456
MflI	RGATCY	3 cut(s)	298, 591, 1075
MhlI	GDGCHC	5 cut(s)	48, 493, 672, 939, 1749
MlsI	TGGCCA	1 cut(s)	458
MluCI	AATT	1 cut(s)	820
MluNI	TGGCCA	1 cut(s)	458
MlyI	GAGTC	4 cut(s)	195, 915, 1226, 1532
MmeI	TCCRAC	7 cut(s)	553, 1125, 1152, 1365, 1542, 1629, 1638
Mox20I	TGGCCA	1 cut(s)	458
MscI	TGGCCA	1 cut(s)	458
MseI	TTAA	5 cut(s)	819, 875, 1473, 1614, 1752
Msp20I	TGGCCA	1 cut(s)	458
MspA1I	CMGCKG	3 cut(s)	722, 828, 1081
MspI	CCGG	4 cut(s)	284, 972, 1491, 1548
MspR9I	CCNGG	5 cut(s)	188, 428, 966, 1492, 1549
Mva1269I	GAATGC	1 cut(s)	987
MvaI	CCWGG	3 cut(s)	188, 428, 966
MvnI	CGCG	3 cut(s)	511, 1218, 1535
NciI	CCSGG	2 cut(s)	1492, 1549
NdeII	GATC	5 cut(s)	298, 591, 1075, 1281, 1354
NlaIII	CATG	4 cut(s)	220, 664, 1051, 1117
NmuCI	GTSAC	4 cut(s)	167, 1229, 1405, 1687
NspI	RCATGY	2 cut(s)	220, 1117
PciSI	GCTCTTC	1 cut(s)	620
PcsI	WCGNNNNNNNCGW	2 cut(s)	843, 1241
PctI	GAATGC	1 cut(s)	987
PfeI	GAWTC	2 cut(s)	861, 1207
PflMI	CCANNNNNTGG	3 cut(s)	895, 965, 1706
PinAI	ACCGGT	1 cut(s)	283
PkrI	GCNGC	8 cut(s)	40, 256, 280, 424, 790, 1339, 1593, 1629
PleI	GAGTC	4 cut(s)	195, 915, 1226, 1532
PmaCI	CACGTG	2 cut(s)	309, 1588
PmlI	CACGTG	2 cut(s)	309, 1588
PpsI	GAGTC	4 cut(s)	195, 915, 1226, 1532
Ppu21I	YACGTR	3 cut(s)	309, 1261, 1588
PpuMI	RGGWCCY	1 cut(s)	679
PshAI	GACNNNNGTC	1 cut(s)	960
PshBI	ATTAAT	1 cut(s)	1752
Psp124BI	GAGCTC	2 cut(s)	672, 1749
Psp1406I	AACGTT	1 cut(s)	1367
Psp5II	RGGWCCY	1 cut(s)	679
Psp6I	CCWGG	3 cut(s)	186, 426, 964
PspCI	CACGTG	2 cut(s)	309, 1588
PspFI	CCCAGC	2 cut(s)	236, 1571
PspGI	CCWGG	3 cut(s)	186, 426, 964
PspPI	GGNCC	8 cut(s)	190, 314, 336, 401, 679, 831, 1053, 1715
PspPPI	RGGWCCY	1 cut(s)	679
PsuI	RGATCY	3 cut(s)	298, 591, 1075
PvuII	CAGCTG	1 cut(s)	722
RsaI	GTAC	7 cut(s)	98, 638, 731, 887, 1147, 1259, 1265
RsaNI	GTAC	7 cut(s)	97, 637, 730, 886, 1146, 1258, 1264
SacI	GAGCTC	2 cut(s)	672, 1749
SalI	GTCGAC	3 cut(s)	845, 1106, 1318
SapI	GCTCTTC	1 cut(s)	620
SaqAI	TTAA	5 cut(s)	819, 875, 1473, 1614, 1752
SatI	GCNGC	8 cut(s)	39, 255, 279, 423, 789, 1338, 1592, 1628
Sau3AI	GATC	5 cut(s)	298, 591, 1075, 1281, 1354
Sau96I	GGNCC	8 cut(s)	190, 314, 336, 401, 679, 831, 1053, 1715
SchI	GAGTC	4 cut(s)	195, 915, 1226, 1532
ScrFI	CCNGG	5 cut(s)	188, 428, 966, 1492, 1549
SduI	GDGCHC	5 cut(s)	48, 493, 672, 939, 1749
SfaNI	GCATC	3 cut(s)	474, 802, 1651
SfcI	CTRYAG	1 cut(s)	940
SgrDI	CGTCGACG	1 cut(s)	845
SinI	GGWCC	4 cut(s)	314, 336, 679, 831
Sse9I	AATT	1 cut(s)	820
SspI	AATATT	2 cut(s)	1003, 1612
SspMI	CTAG	3 cut(s)	182, 194, 414
SstI	GAGCTC	2 cut(s)	672, 1749
StyD4I	CCNGG	5 cut(s)	186, 426, 964, 1490, 1547
TaaI	ACNGT	4 cut(s)	251, 608, 1383, 1504
TaiI	ACGT	9 cut(s)	311, 435, 758, 846, 1107, 1263, 1370, 1518, 1590
TaqII	GACCGA	2 cut(s)	411, 848
TasI	AATT	1 cut(s)	820
TatI	WGTACW	1 cut(s)	729
TauI	GCSGC	4 cut(s)	41, 1340, 1594, 1630
TfiI	GAWTC	2 cut(s)	861, 1207
Tru1I	TTAA	5 cut(s)	819, 875, 1473, 1614, 1752
Tru9I	TTAA	5 cut(s)	819, 875, 1473, 1614, 1752
TscAI	CASTG	4 cut(s)	545, 754, 1386, 1548
TseFI	GTSAC	4 cut(s)	167, 1229, 1405, 1687
TseI	GCWGC	4 cut(s)	254, 278, 422, 788
Tsp45I	GTSAC	4 cut(s)	167, 1229, 1405, 1687
TspDTI	ATGAA	3 cut(s)	96, 189, 1335
TspGWI	ACGGA	2 cut(s)	1163, 1193
TspRI	CASTG	4 cut(s)	545, 754, 1386, 1548
Van91I	CCANNNNNTGG	3 cut(s)	895, 965, 1706
VneI	GTGCAC	1 cut(s)	44
VpaK11BI	GGWCC	4 cut(s)	314, 336, 679, 831
VspI	ATTAAT	1 cut(s)	1752
XagI	CCTNNNNNAGG	2 cut(s)	185, 396
XceI	RCATGY	2 cut(s)	220, 1117
XmiI	GTMKAC	3 cut(s)	846, 1107, 1319
XspI	CTAG	3 cut(s)	182, 194, 414

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.