Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0006919

Physical Location & Seq

Genomic Coordinates Forward (+)

26805608 .. 26807440

Gene Structure 1833 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g25140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1833 bp

ATGTCTTCTCCTTTGCGAACATCTGCATTATCCTCCATCTCTTCCTTCCGTCGTCCTCAGCCTTTTCCAAAGAAGTGCCAAAACACTTGTTCAGTTGGAAAACCAAGGGTTTGCTTTGTGGGTAGGTTACCGCCATGCAAAGCAACAATTAATAGTGATCAGAACCCCAGTAACCAAGACCCTAGGGCACCTCATGTGGGAAAAATATTAGACAGGAGAAATGTACTGATTGGTCTAGGAGCTGGAGGGCTATATGGCGCGGCCGGTCTCGGCCCCAGCCGATTCGCATTTGCTGATCCAATTCCACCGCCCGACATAAACACGTGCACTGAGACGACCGACCCCAAAATACCAACACAAGAGTGTTGCCCGCCAAAACCTGATAAAATCAAAATCATTGATTTTCAGCTACCGGCGTCACAACCCGCAATACTACGCAAGAGGCGTGCAGCTCAGGATGCCGCGAAGGACCGGGTGTATGTAGCAAACTACAAAGAGGCCATTAGGCGCATGAAGTCCCTTGACATAAACGATCCACGTAGTTTCTGGAGCCAAGCTAATGTTCACTGTGCCTATTGTGAGGGCTCATATCATTACGGTGAAAAGAAATTAGAAATACAAGTGCACTCCTCGTGGCTTTTCTATCCCTTCCACCGCTTGTACTTGTACTTCTACGAGCGAATCCTGGCGTCGTTGATTAAGGAACGCGACCCTAATTTTGCTTTGCCCTTTTGGAACTGGGATGCTCCAGAAGGGATGTACATGCCAACCATATTTGAGGAGGATGGTGTAATTAACCCTCTCTATGATCCCTACCGAAATGACAAGCACCGGCGTCCAGGGACAATCGTGGACCTCAACTACGGGCTAGGCATGGACAACAACACCCGAGACACTGACACAATAGAGAATTATAATCTCTTCACCATGCACAACAAAATGTTGAGTGGTGCTCGCTGGGACTGGTGCTTATTCTTTGGCCATCCTTACAGGGCAGGGGACAACCCAAATCCAGGAGCCGGCAACATCGAGCTTGTTCCCCATAACACCGTCCACGATTGGACTGGCACTACTCAAGACTCAACCCAGGGTGGGGTGGACATGGGGATATTCTACTCTGCAGGTAGAGATCCAGTGTTTTACGCTCACCATGCCAACGTGGACCGCATGTGGTACCTGTGGAAAAACAGATTCGGAGGCAAGGATATCAAGGACCTTGATTGGCTCGACACCGAGTTTCTATTCTACGACGAGAACAAGCAGCTAATGCGTGTTAAGGTCCGAGATTCTCTTGACACCGCCCTCCTTGGGTACGAATACCAAAGCGTTGAAATTCCATGGATTGATGACAACTTCAAGCCCACCGCTCGCTTTCCAACCAACAAGACAAAACCACAAGTTTTATTTACTGAGCATCACACCAAGTTCCCGTTTACCTTGGACTCCACGATCAGTGTCGAGGTGGCGAGGCCAGTGCCGAGAAGGCGAAGCGACTCTGAGAAGGCTAAACAGCAGGAGGTGTTGGTGGTCGGTGGGATTGGGTTCCCAGCTAACGAGAGTGTGAAGTTTGATGTGTACGTGAATGATGACGCGGACTCGCCCAGTGGACTGGACAAGTCTGAGTTTGCCGGGAGCTTTGTGCATATCCCGCACAGGCACAAAGAAAAGATTCAAACCAAGCTGACGTTAGGGATCACACAGCTGTTGGAGGATTTGCGTGCGGAAGAGGACAGCAGTGTGGTGGTGACTTTGGTCCCTAGGGTTGGGATAGGAAAGGTCACCCTTTCTTGCATAACCATCGAGCTCTCTGCTTGTCCGAATCAGGCTCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

611

Amino Acids

69.17

Weight (kDa)

6.99

Isoelectric Point (pI)

52.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	180 - 396	2.5e-33	Common central domain of tyrosinase
PPO1_DWL	PF12142	402 - 450	2.7e-22	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	476 - 602	3.5e-44	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g04680
malus_domestica	MD05G1319800.v1.1 MD05G1320100.v1.1 MD05G1320200.v1.1 MD05G1320500.v1.1 MD05G1320800.v1.1 MD10G1298300.v1.1
prunus_persica	Prupe.4G041500_v2.0.a1 Prupe.4G041700_v2.0.a1
pyrus_communis	pycom05g29530 pycom05g29560 pycom10g24890 pycom10g24910 pycom10g25140
rosa_chinensis	RchiOBHm_Chr5g0007621
rosa_laevigata	RLG00000031479
rosa_multiflora	Rmu_co8439317.1_g000001 Rmu_sc0011744.1_g000006 Rmu_sc0021005.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068730
rosa_rugosa	Rorug04G0429400
rosa_samantha	Rh5AG057500 Rh5BG054400 Rh5CG064400 Rh5DG053600
rosa_wichuraiana	Rw5G006180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	915
Acc36I	ACCTGC	1 cut(s)	1112
Acc65I	GGTACC	1 cut(s)	1173
AccB1I	GGYRCC	2 cut(s)	187, 1173
AccBSI	CCGCTC	1 cut(s)	1367
AccII	CGCG	4 cut(s)	260, 464, 708, 1592
AclWI	GGATC	5 cut(s)	290, 527, 803, 1124, 1700
AcoI	YGGCCR	2 cut(s)	261, 979
AcsI	RAATTY	1 cut(s)	1332
AcvI	CACGTG	1 cut(s)	324
AcyI	GRCGYC	3 cut(s)	416, 689, 835
AfaI	GTAC	7 cut(s)	225, 662, 668, 761, 1175, 1313, 1577
AfiI	CCNNNNNNNGG	7 cut(s)	197, 1013, 1019, 1092, 1093, 1308, 1763
AflIII	ACRYGT	1 cut(s)	321
AgsI	TTSAA	3 cut(s)	1331, 1357, 1673
AjnI	CCWGG	4 cut(s)	684, 838, 1012, 1086
AjuI	GAANNNNNNNTTGG	2 cut(s)	546, 578
AleI	CACNNNNGTG	1 cut(s)	361
Alw21I	GWGCWC	4 cut(s)	329, 627, 955, 1806
Alw26I	GTCTC	3 cut(s)	272, 326, 885
Alw44I	GTGCAC	2 cut(s)	325, 623
AlwI	GGATC	5 cut(s)	290, 527, 803, 1124, 1700
Ama87I	CYCGRG	1 cut(s)	888
AoxI	GGCC	5 cut(s)	261, 271, 498, 979, 1469
ApaLI	GTGCAC	2 cut(s)	325, 623
ApeKI	GCWGC	2 cut(s)	449, 1261
ApoI	RAATTY	1 cut(s)	1332
ArsI	GACNNNNNNTTYG	2 cut(s)	701, 733
AseI	ATTAAT	1 cut(s)	150
Asp700I	GAANNNNTTC	1 cut(s)	1668
Asp718I	GGTACC	1 cut(s)	1173
AspA2I	CCTAGG	2 cut(s)	182, 1757
AspLEI	GCGC	2 cut(s)	260, 510
AspS9I	GGNCC	7 cut(s)	272, 469, 853, 1162, 1213, 1279, 1753
AsuC2I	CCSGG	2 cut(s)	473, 1630
AsuHPI	GGTGA	5 cut(s)	611, 916, 1139, 1756, 1771
AvaI	CYCGRG	1 cut(s)	888
AvaII	GGWCC	6 cut(s)	469, 853, 1162, 1213, 1279, 1753
AvrII	CCTAGG	2 cut(s)	182, 1757
BaeGI	GKGCMC	3 cut(s)	190, 329, 627
BalI	TGGCCA	1 cut(s)	981
BanI	GGYRCC	2 cut(s)	187, 1173
BanII	GRGCYC	2 cut(s)	587, 1806
BarI	GAAGNNNNNNTAC	2 cut(s)	653, 685
BauI	CACGAG	1 cut(s)	631
BbrPI	CACGTG	1 cut(s)	324
Bbv12I	GWGCWC	4 cut(s)	329, 627, 955, 1806
BbvCI	CCTCAGC	1 cut(s)	57
BbvI	GCAGC	2 cut(s)	461, 1273
BccI	CCATC	4 cut(s)	44, 779, 990, 1805
BcgI	CGANNNNNNTGC	6 cut(s)	1456, 1490, 1780, 1790, 1814, 1824
BciT130I	CCWGG	4 cut(s)	686, 840, 1014, 1088
BclI	TGATCA	1 cut(s)	157
BcnI	CCSGG	2 cut(s)	473, 1630
BcoDI	GTCTC	3 cut(s)	272, 326, 885
BfaI	CTAG	4 cut(s)	183, 236, 869, 1758
BfmI	CTRYAG	1 cut(s)	1119
BfuAI	ACCTGC	1 cut(s)	1112
BglI	GCCNNNNNGGC	1 cut(s)	1483
BisI	GCNGC	4 cut(s)	261, 450, 462, 1262
BlnI	CCTAGG	2 cut(s)	182, 1757
BlsI	GCNGC	4 cut(s)	262, 451, 463, 1263
Bme1390I	CCNGG	6 cut(s)	473, 686, 840, 1014, 1088, 1630
Bme18I	GGWCC	6 cut(s)	469, 853, 1162, 1213, 1279, 1753
BmeT110I	CYCGRG	1 cut(s)	888
BmgT120I	GGNCC	7 cut(s)	272, 469, 853, 1162, 1213, 1279, 1753
BmiI	GGNNCC	7 cut(s)	189, 274, 551, 1018, 1175, 1544, 1755
BmrFI	CCNGG	6 cut(s)	473, 686, 840, 1014, 1088, 1630
BmrI	ACTGGG	3 cut(s)	162, 748, 1596
BmsI	GCATC	3 cut(s)	448, 733, 1423
BmuI	ACTGGG	3 cut(s)	162, 748, 1596
BoxI	GACNNNNGTC	1 cut(s)	1751
BplI	GAGNNNNNCTC	2 cut(s)	937, 969
BpmI	CTGGAG	3 cut(s)	264, 568, 732
Bpu10I	CCTNAGC	2 cut(s)	57, 453
BpuEI	CTTGAG	1 cut(s)	1059
BpuMI	CCSGG	2 cut(s)	473, 1630
BsaAI	YACGTR	3 cut(s)	324, 539, 1579
BsaHI	GRCGYC	3 cut(s)	416, 689, 835
BsaI	GGTCTC	1 cut(s)	272
BsaJI	CCNNGG	9 cut(s)	104, 182, 839, 1086, 1087, 1306, 1337, 1437, 1757
Bsc4I	CCNNNNNNNGG	7 cut(s)	197, 1013, 1019, 1092, 1093, 1308, 1763
Bse118I	RCCGGY	4 cut(s)	263, 412, 831, 1019
Bse1I	ACTGG	8 cut(s)	168, 743, 968, 1069, 1133, 1472, 1602, 1614
BseBI	CCWGG	4 cut(s)	686, 840, 1014, 1088
BseDI	CCNNGG	9 cut(s)	104, 182, 839, 1086, 1087, 1306, 1337, 1437, 1757
BseGI	GGATG	5 cut(s)	463, 748, 762, 790, 982
BseLI	CCNNNNNNNGG	7 cut(s)	197, 1013, 1019, 1092, 1093, 1308, 1763
BseMII	CTCAG	6 cut(s)	71, 321, 467, 1401, 1488, 1611
BseNI	ACTGG	8 cut(s)	168, 743, 968, 1069, 1133, 1472, 1602, 1614
BseRI	GAGGAG	2 cut(s)	619, 794
BseSI	GKGCMC	3 cut(s)	190, 329, 627
BseX3I	CGGCCG	1 cut(s)	261
BseXI	GCAGC	2 cut(s)	461, 1273
BseYI	CCCAGC	3 cut(s)	275, 957, 1546
BsgI	GTGCAG	1 cut(s)	468
Bsh1236I	CGCG	4 cut(s)	260, 464, 708, 1592
Bsh1285I	CGRYCG	2 cut(s)	264, 339
BshFI	GGCC	5 cut(s)	263, 273, 500, 981, 1471
BshNI	GGYRCC	2 cut(s)	187, 1173
BsiEI	CGRYCG	2 cut(s)	264, 339
BsiHKAI	GWGCWC	4 cut(s)	329, 627, 955, 1806
BsiHKCI	CYCGRG	1 cut(s)	888
BsiSI	CCGG	6 cut(s)	264, 413, 472, 832, 1020, 1629
BslFI	GGGAC	5 cut(s)	502, 856, 974, 1013, 1739
BslI	CCNNNNNNNGG	7 cut(s)	197, 1013, 1019, 1092, 1093, 1308, 1763
BsmAI	GTCTC	3 cut(s)	272, 326, 885
BsmBI	CGTCTC	1 cut(s)	326
BsmFI	GGGAC	5 cut(s)	502, 856, 974, 1013, 1739
BsnI	GGCC	5 cut(s)	263, 273, 500, 981, 1471
Bso31I	GGTCTC	1 cut(s)	272
BsoBI	CYCGRG	1 cut(s)	888
Bsp1286I	GDGCHC	6 cut(s)	190, 329, 587, 627, 955, 1806
Bsp1407I	TGTACA	1 cut(s)	759
Bsp143I	GATC	7 cut(s)	157, 295, 532, 808, 1129, 1449, 1692
Bsp19I	CCATGG	1 cut(s)	1337
BspANI	GGCC	5 cut(s)	263, 273, 500, 981, 1471
BspCNI	CTCAG	6 cut(s)	70, 322, 466, 1402, 1489, 1612
BspFNI	CGCG	4 cut(s)	260, 464, 708, 1592
BspLI	GGNNCC	7 cut(s)	189, 274, 551, 1018, 1175, 1544, 1755
BspMAI	CTGCAG	1 cut(s)	1123
BspMI	ACCTGC	1 cut(s)	1112
BspPI	GGATC	5 cut(s)	290, 527, 803, 1124, 1700
BspT107I	GGYRCC	2 cut(s)	187, 1173
BspTNI	GGTCTC	1 cut(s)	272
BsrBI	CCGCTC	1 cut(s)	1367
BsrFI	RCCGGY	4 cut(s)	263, 412, 831, 1019
BsrGI	TGTACA	1 cut(s)	759
BsrI	ACTGG	8 cut(s)	168, 743, 968, 1069, 1133, 1472, 1602, 1614
BssAI	RCCGGY	4 cut(s)	263, 412, 831, 1019
BssECI	CCNNGG	9 cut(s)	104, 182, 839, 1086, 1087, 1306, 1337, 1437, 1757
BssMI	GATC	7 cut(s)	157, 295, 532, 808, 1129, 1449, 1692
BssNI	GRCGYC	3 cut(s)	416, 689, 835
BssSI	CACGAG	1 cut(s)	631
BssT1I	CCWWGG	6 cut(s)	104, 182, 1306, 1337, 1437, 1757
Bst2BI	CACGAG	1 cut(s)	631
Bst2UI	CCWGG	4 cut(s)	686, 840, 1014, 1088
Bst4CI	ACNGT	3 cut(s)	569, 599, 1051
Bst6I	CTCTTC	3 cut(s)	46, 926, 1719
BstACI	GRCGYC	3 cut(s)	416, 689, 835
BstAPI	GCANNNNNTGC	1 cut(s)	1267
BstAUI	TGTACA	1 cut(s)	759
BstBAI	YACGTR	3 cut(s)	324, 539, 1579
BstC8I	GCNNGC	6 cut(s)	371, 447, 955, 1021, 1369, 1719
BstDEI	CTNAG	6 cut(s)	57, 330, 453, 1410, 1497, 1620
BstDSI	CCRYGG	1 cut(s)	1337
BstEII	GGTNACC	2 cut(s)	126, 1777
BstF5I	GGATG	5 cut(s)	463, 748, 762, 790, 982
BstFNI	CGCG	4 cut(s)	260, 464, 708, 1592
BstHHI	GCGC	2 cut(s)	260, 510
BstKTI	GATC	7 cut(s)	160, 298, 535, 811, 1132, 1452, 1695
BstMAI	GTCTC	3 cut(s)	272, 326, 885
BstMBI	GATC	7 cut(s)	157, 295, 532, 808, 1129, 1449, 1692
BstMCI	CGRYCG	2 cut(s)	264, 339
BstMWI	GCNNNNNNNGC	5 cut(s)	458, 1151, 1267, 1483, 1648
BstNI	CCWGG	4 cut(s)	686, 840, 1014, 1088
BstNSI	RCATGY	2 cut(s)	766, 1171
BstPAI	GACNNNNGTC	1 cut(s)	1751
BstPI	GGTNACC	2 cut(s)	126, 1777
BstSCI	CCNGG	6 cut(s)	471, 684, 838, 1012, 1086, 1628
BstSFI	CTRYAG	1 cut(s)	1119
BstSLI	GKGCMC	3 cut(s)	190, 329, 627
BstUI	CGCG	4 cut(s)	260, 464, 708, 1592
BstV1I	GCAGC	2 cut(s)	461, 1273
BstX2I	RGATCY	1 cut(s)	1129
BstXI	CCANNNNNNTGG	1 cut(s)	1609
BstYI	RGATCY	1 cut(s)	1129
BstZI	CGGCCG	1 cut(s)	261
BsuRI	GGCC	5 cut(s)	263, 273, 500, 981, 1471
BtgI	CCRYGG	1 cut(s)	1337
BtsCI	GGATG	5 cut(s)	463, 748, 762, 790, 982
BtsI	GCAGTG	1 cut(s)	1741
BtsIMutI	CAGTG	8 cut(s)	327, 565, 894, 1140, 1459, 1479, 1609, 1741
BveI	ACCTGC	1 cut(s)	1112
Cac8I	GCNNGC	6 cut(s)	371, 447, 955, 1021, 1369, 1719
CfoI	GCGC	2 cut(s)	260, 510
Cfr10I	RCCGGY	4 cut(s)	263, 412, 831, 1019
Cfr13I	GGNCC	7 cut(s)	272, 469, 853, 1162, 1213, 1279, 1753
CseI	GACGC	4 cut(s)	405, 678, 824, 1598
Csp6I	GTAC	7 cut(s)	224, 661, 667, 760, 1174, 1312, 1576
CviQI	GTAC	7 cut(s)	224, 661, 667, 760, 1174, 1312, 1576
DdeI	CTNAG	6 cut(s)	57, 330, 453, 1410, 1497, 1620
DpnI	GATC	7 cut(s)	159, 297, 534, 810, 1131, 1451, 1694
DpnII	GATC	7 cut(s)	157, 295, 532, 808, 1129, 1449, 1692
EaeI	YGGCCR	2 cut(s)	261, 979
EagI	CGGCCG	1 cut(s)	261
Eam1104I	CTCTTC	3 cut(s)	46, 926, 1719
EarI	CTCTTC	3 cut(s)	46, 926, 1719
Ecl136II	GAGCTC	1 cut(s)	1804
EclXI	CGGCCG	1 cut(s)	261
Eco130I	CCWWGG	6 cut(s)	104, 182, 1306, 1337, 1437, 1757
Eco24I	GRGCYC	2 cut(s)	587, 1806
Eco31I	GGTCTC	1 cut(s)	272
Eco32I	GATATC	1 cut(s)	1207
Eco47I	GGWCC	6 cut(s)	469, 853, 1162, 1213, 1279, 1753
Eco52I	CGGCCG	1 cut(s)	261
Eco53kI	GAGCTC	1 cut(s)	1804
Eco72I	CACGTG	1 cut(s)	324
Eco88I	CYCGRG	1 cut(s)	888
Eco91I	GGTNACC	2 cut(s)	126, 1777
EcoICRI	GAGCTC	1 cut(s)	1804
EcoO109I	RGGNCCY	1 cut(s)	1213
EcoO65I	GGTNACC	2 cut(s)	126, 1777
EcoRII	CCWGG	4 cut(s)	684, 838, 1012, 1086
EcoRV	GATATC	1 cut(s)	1207
EcoT14I	CCWWGG	6 cut(s)	104, 182, 1306, 1337, 1437, 1757
EcoT38I	GRGCYC	2 cut(s)	587, 1806
ErhI	CCWWGG	6 cut(s)	104, 182, 1306, 1337, 1437, 1757
Esp3I	CGTCTC	1 cut(s)	326
FaqI	GGGAC	5 cut(s)	502, 856, 974, 1013, 1739
FauI	CCCGC	3 cut(s)	378, 433, 1656
FbaI	TGATCA	1 cut(s)	157
Fnu4HI	GCNGC	4 cut(s)	261, 450, 462, 1262
FokI	GGATG	5 cut(s)	470, 755, 769, 797, 969
FriOI	GRGCYC	2 cut(s)	587, 1806
Fsp4HI	GCNGC	4 cut(s)	261, 450, 462, 1262
FspBI	CTAG	4 cut(s)	183, 236, 869, 1758
GlaI	GCGC	2 cut(s)	259, 509
GluI	GCNGC	4 cut(s)	261, 450, 462, 1262
GsaI	CCCAGC	3 cut(s)	279, 961, 1550
GsuI	CTGGAG	3 cut(s)	264, 568, 732
HaeIII	GGCC	5 cut(s)	263, 273, 500, 981, 1471
HapII	CCGG	6 cut(s)	264, 413, 472, 832, 1020, 1629
HgaI	GACGC	4 cut(s)	405, 678, 824, 1598
HhaI	GCGC	2 cut(s)	260, 510
Hin1I	GRCGYC	3 cut(s)	416, 689, 835
Hin6I	GCGC	2 cut(s)	258, 508
HinP1I	GCGC	2 cut(s)	258, 508
HpaII	CCGG	6 cut(s)	264, 413, 472, 832, 1020, 1629
HphI	GGTGA	5 cut(s)	611, 916, 1139, 1756, 1771
Hpy188I	TCNGA	7 cut(s)	162, 1196, 1283, 1498, 1621, 1818, 1832
Hpy188III	TCNNGA	5 cut(s)	455, 547, 749, 1076, 1292
Hpy99I	CGWCG	3 cut(s)	54, 694, 1253
HpyAV	CCTTC	6 cut(s)	55, 460, 658, 746, 1476, 1495
HpyCH4III	ACNGT	3 cut(s)	569, 599, 1051
HpyCH4IV	ACGT	5 cut(s)	323, 538, 1158, 1578, 1685
HpyCH4V	TGCA	9 cut(s)	26, 138, 327, 449, 625, 931, 1121, 1642, 1791
HpyF10VI	GCNNNNNNNGC	5 cut(s)	458, 1151, 1267, 1483, 1648
HpyF3I	CTNAG	6 cut(s)	57, 330, 453, 1410, 1497, 1620
HpySE526I	ACGT	5 cut(s)	323, 538, 1158, 1578, 1685
Hsp92I	GRCGYC	3 cut(s)	416, 689, 835
HspAI	GCGC	2 cut(s)	258, 508
KpnI	GGTACC	1 cut(s)	1177
KroI	GCCGGC	1 cut(s)	1019
KroNI	GCCGGC	1 cut(s)	1021
Ksp22I	TGATCA	1 cut(s)	157
Kzo9I	GATC	7 cut(s)	157, 295, 532, 808, 1129, 1449, 1692
LmnI	GCTCC	5 cut(s)	239, 549, 751, 1016, 1632
Lsp1109I	GCAGC	2 cut(s)	461, 1273
LweI	GCATC	3 cut(s)	448, 733, 1423
MaeI	CTAG	4 cut(s)	183, 236, 869, 1758
MaeII	ACGT	5 cut(s)	323, 538, 1158, 1578, 1685
MaeIII	GTNAC	5 cut(s)	126, 170, 417, 1744, 1777
MalI	GATC	7 cut(s)	159, 297, 534, 810, 1131, 1451, 1694
MbiI	CCGCTC	1 cut(s)	1367
MboI	GATC	7 cut(s)	157, 295, 532, 808, 1129, 1449, 1692
MboII	GAAGA	3 cut(s)	33, 913, 1736
MflI	RGATCY	1 cut(s)	1129
MhlI	GDGCHC	6 cut(s)	190, 329, 587, 627, 955, 1806
MlsI	TGGCCA	1 cut(s)	981
MluCI	AATT	7 cut(s)	147, 300, 608, 715, 792, 910, 1332
MluNI	TGGCCA	1 cut(s)	981
MlyI	GAGTC	4 cut(s)	1073, 1436, 1487, 1589
MmeI	TCCRAC	3 cut(s)	76, 1400, 1686
Mox20I	TGGCCA	1 cut(s)	981
MroNI	GCCGGC	1 cut(s)	1019
MroXI	GAANNNNTTC	1 cut(s)	1668
MscI	TGGCCA	1 cut(s)	981
MseI	TTAA	4 cut(s)	150, 699, 795, 1275
MslI	CAYNNNNRTG	2 cut(s)	361, 597
Msp20I	TGGCCA	1 cut(s)	981
MspA1I	CMGCKG	1 cut(s)	1702
MspI	CCGG	6 cut(s)	264, 413, 472, 832, 1020, 1629
MspR9I	CCNGG	6 cut(s)	473, 686, 840, 1014, 1088, 1630
MvaI	CCWGG	4 cut(s)	686, 840, 1014, 1088
MvnI	CGCG	4 cut(s)	260, 464, 708, 1592
MwoI	GCNNNNNNNGC	5 cut(s)	458, 1151, 1267, 1483, 1648
NaeI	GCCGGC	1 cut(s)	1021
NciI	CCSGG	2 cut(s)	473, 1630
NcoI	CCATGG	1 cut(s)	1337
NdeII	GATC	7 cut(s)	157, 295, 532, 808, 1129, 1449, 1692
NgoMIV	GCCGGC	1 cut(s)	1019
NlaIV	GGNNCC	7 cut(s)	189, 274, 551, 1018, 1175, 1544, 1755
NmeAIII	GCCGAG	2 cut(s)	249, 1503
NmuCI	GTSAC	3 cut(s)	417, 1744, 1777
NspI	RCATGY	2 cut(s)	766, 1171
OliI	CACNNNNGTG	1 cut(s)	361
PasI	CCCWGGG	1 cut(s)	1087
PcsI	WCGNNNNNNNCGW	1 cut(s)	442
PdiI	GCCGGC	1 cut(s)	1021
PdmI	GAANNNNTTC	1 cut(s)	1668
PfeI	GAWTC	6 cut(s)	282, 681, 1191, 1286, 1669, 1819
PfoI	TCCNGGA	1 cut(s)	1012
PkrI	GCNGC	4 cut(s)	262, 451, 463, 1263
PleI	GAGTC	4 cut(s)	1073, 1436, 1487, 1589
PmaCI	CACGTG	1 cut(s)	324
PmlI	CACGTG	1 cut(s)	324
PpsI	GAGTC	4 cut(s)	1073, 1436, 1487, 1589
Ppu21I	YACGTR	3 cut(s)	324, 539, 1579
PpuMI	RGGWCCY	1 cut(s)	1213
PshAI	GACNNNNGTC	1 cut(s)	1751
PshBI	ATTAAT	1 cut(s)	150
PsiI	TTATAA	1 cut(s)	915
Psp124BI	GAGCTC	1 cut(s)	1806
Psp5II	RGGWCCY	1 cut(s)	1213
Psp6I	CCWGG	4 cut(s)	684, 838, 1012, 1086
PspCI	CACGTG	1 cut(s)	324
PspEI	GGTNACC	2 cut(s)	126, 1777
PspFI	CCCAGC	3 cut(s)	275, 957, 1546
PspGI	CCWGG	4 cut(s)	684, 838, 1012, 1086
PspN4I	GGNNCC	7 cut(s)	189, 274, 551, 1018, 1175, 1544, 1755
PspPI	GGNCC	7 cut(s)	272, 469, 853, 1162, 1213, 1279, 1753
PspPPI	RGGWCCY	1 cut(s)	1213
PstI	CTGCAG	1 cut(s)	1123
PsuI	RGATCY	1 cut(s)	1129
PvuII	CAGCTG	1 cut(s)	1702
RsaI	GTAC	7 cut(s)	225, 662, 668, 761, 1175, 1313, 1577
RsaNI	GTAC	7 cut(s)	224, 661, 667, 760, 1174, 1312, 1576
RseI	CAYNNNNRTG	2 cut(s)	361, 597
SacI	GAGCTC	1 cut(s)	1806
SaqAI	TTAA	4 cut(s)	150, 699, 795, 1275
SatI	GCNGC	4 cut(s)	261, 450, 462, 1262
Sau3AI	GATC	7 cut(s)	157, 295, 532, 808, 1129, 1449, 1692
Sau96I	GGNCC	7 cut(s)	272, 469, 853, 1162, 1213, 1279, 1753
SchI	GAGTC	4 cut(s)	1073, 1436, 1487, 1589
ScrFI	CCNGG	6 cut(s)	473, 686, 840, 1014, 1088, 1630
SduI	GDGCHC	6 cut(s)	190, 329, 587, 627, 955, 1806
SfaNI	GCATC	3 cut(s)	448, 733, 1423
SfcI	CTRYAG	1 cut(s)	1119
SgrAI	CRCCGGYG	1 cut(s)	831
SinI	GGWCC	6 cut(s)	469, 853, 1162, 1213, 1279, 1753
SmiMI	CAYNNNNRTG	2 cut(s)	361, 597
SmlI	CTYRAG	1 cut(s)	1074
SmoI	CTYRAG	1 cut(s)	1074
Sse9I	AATT	7 cut(s)	147, 300, 608, 715, 792, 910, 1332
SspI	AATATT	1 cut(s)	207
SspMI	CTAG	4 cut(s)	183, 236, 869, 1758
SstI	GAGCTC	1 cut(s)	1806
StyD4I	CCNGG	6 cut(s)	471, 684, 838, 1012, 1086, 1628
StyI	CCWWGG	6 cut(s)	104, 182, 1306, 1337, 1437, 1757
TaaI	ACNGT	3 cut(s)	569, 599, 1051
TaiI	ACGT	5 cut(s)	326, 541, 1161, 1581, 1688
TaqI	TCGA	4 cut(s)	1029, 1227, 1458, 1800
TaqII	GACCGA	1 cut(s)	353
TasI	AATT	7 cut(s)	147, 300, 608, 715, 792, 910, 1332
TatI	WGTACW	4 cut(s)	223, 660, 666, 759
TauI	GCSGC	2 cut(s)	263, 464
TfiI	GAWTC	6 cut(s)	282, 681, 1191, 1286, 1669, 1819
Tru1I	TTAA	4 cut(s)	150, 699, 795, 1275
Tru9I	TTAA	4 cut(s)	150, 699, 795, 1275
TscAI	CASTG	8 cut(s)	334, 572, 901, 1140, 1459, 1479, 1609, 1741
TseFI	GTSAC	3 cut(s)	417, 1744, 1777
TseI	GCWGC	2 cut(s)	449, 1261
Tsp45I	GTSAC	3 cut(s)	417, 1744, 1777
TspDTI	ATGAA	1 cut(s)	527
TspGWI	ACGGA	1 cut(s)	38
TspRI	CASTG	8 cut(s)	334, 572, 901, 1140, 1459, 1479, 1609, 1741
VneI	GTGCAC	2 cut(s)	325, 623
VpaK11BI	GGWCC	6 cut(s)	469, 853, 1162, 1213, 1279, 1753
VspI	ATTAAT	1 cut(s)	150
XapI	RAATTY	1 cut(s)	1332
XceI	RCATGY	2 cut(s)	766, 1171
XcmI	CCANNNNNNNNNTGG	2 cut(s)	543, 1061
XmaJI	CCTAGG	2 cut(s)	182, 1757
XmnI	GAANNNNTTC	1 cut(s)	1668
XspI	CTAG	4 cut(s)	183, 236, 869, 1758

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.