Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006919

Physical Location & Seq

Genomic Coordinates Forward (+)

44810753 .. 44812510

Gene Structure 1758 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1320800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGTCTTCATCTCCCTTACCAACTTCTACAATGGTCGCCCTGCACTCCACCACAACCACCTTCTTCCGCTCTCATTTATTCCCAAACAAGTCCCAGACTCCACTGCAACGAAAACCCAAACAATGCCTTGCGGGTAGAGTGCGCTGCAAAGCAACAAAAGGTGACAATGATAACCTAGATCAGGGCCTAGCAAGACTCGACAGGAGAAACATGCTGATAGGTTTAGGCGCCGGGGGTCTCTACGGTAAGGTAGGAAACCCATTTGCTTTTGCCGCACCAATATCCGCCCCAGACTTGACCACGTGTGTCCCTGCCAGCAAGCCAGACGGGTCCACCATCGATTGTTGCCCACCTACCACCACGACCATCATCGACTTTGAACTCCCTGACTCAGGCCCACTCCGCACTAGGCCCGCTGCCCAGGACGTTGCAAATGACCCTGAATACTTGGCCAAATACAAAAATGCCGTCGAGATGATGCGGGCACTTCCAGACGACGACCCGCGCAGTCACGCACAACAGGCGATGGTACACTGCTCATACTGCGACGGTGGATACCCACAAGTTGGATATTCAGATTTGGAGATCCAAGTTCACTTCTGTTGGCTATTCTTCCCGTTCCATCGTTGGTACCTCTACTTCTATGAGAAAATCATGGGCGAGCTCATTGGGGACCAAACCTTCGCCCTCCCCTTCTGGAACTGGGACGCGCCAGCTGGCATGTACATTCCTGAAATTTTCACTGATACGACGTCATCCCTCTACGACCAGTACAGAAATACAGCGCATCAGCCCCCGAAGCTCCTGGACTTGAATTATGGCAGGACCGACGATGACGTCGACGACGCGACACGAATCAAAGAGAACCTAACAACGATGTACAAGCAGATGGTGTCGAATGCCACTTGTCATAGATTATTCTACGGAGAGCCTTACAGCGCAGGGGACGAACCAGATCCTGGCGCCGGAAACATTGAGACCACTCCCCATAACAATATTCACCTTTGGGTTGGCGACCCAACCCAGCCAAACGGGGAGGACATGGGGACCTTTTACTCTGCGGCGAGGGATCCGGTGTTTTACTCTCACCATTCCAACGTGGACCGCATGTGGTCTATATATAAAGATAAGTTGGGTGGTACGGACATAGAAAATACCGATTGGCTGGACGCAGAGTTCTTGTTCTACGACGAGAAAAAGAATCTCATGCGCGTCAATGTTCGGGACTCGCTCGACACGAAAAAACTCGGGTATGTGTACGACGAGAATGTCTCGATCCCGTGGCTGGATTCGAAGCCGACGGCTCGTAAGTCAACAAATAAGAGAAAGGCTACGGTTTCATCTTCTGATCTTTCTACAACGTTCCCTGCTACACTATCAGATATAATCAGCGTCGAGGTGACGAGGCCGTCTAAGACGAAACGGACAGCTGCCCAGAAGAAGGCTCAGGATGAGGTGCTGGTGATTAAGGGGATTGAGTTTGCCGGGAATGAAACTGTGAAGTTCGACGTGTATGTGAACGATGACGCGGACTCGCTGGCCGGGAAAGACAAGTCGGAGTTTGCTGGGAGTTTTGTTCACGTGCCGCATAAGCATAAGAAGAATATTAAGACGAACCTGCGGCTGAGCATTACAAGCTTGTTGGAAGAGTTGGATGCGGAGACAGACAACAGTTTGGTAGTGACTTTGGTGCCGAAAGTTGGGAAGGGGCCAATCACCATCGGAGGGTTTAGCATTGAGCTCATTAATACTACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

586

Amino Acids

65.28

Weight (kDa)

5.57

Isoelectric Point (pI)

40.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	169 - 376	9.4e-36	Common central domain of tyrosinase
PPO1_DWL	PF12142	382 - 434	1.5e-22	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	455 - 581	1.8e-47	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g04680
malus_domestica	MD05G1319800.v1.1 MD05G1320100.v1.1 MD05G1320200.v1.1 MD05G1320500.v1.1 MD05G1320800.v1.1 MD10G1298300.v1.1
prunus_persica	Prupe.4G041500_v2.0.a1 Prupe.4G041700_v2.0.a1
pyrus_communis	pycom05g29530 pycom05g29560 pycom10g24890 pycom10g24910 pycom10g25140
rosa_chinensis	RchiOBHm_Chr5g0007621
rosa_laevigata	RLG00000031479
rosa_multiflora	Rmu_co8439317.1_g000001 Rmu_sc0011744.1_g000006 Rmu_sc0021005.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068730
rosa_rugosa	Rorug04G0429400
rosa_samantha	Rh5AG057500 Rh5BG054400 Rh5CG064400 Rh5DG053600
rosa_wichuraiana	Rw5G006180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	836, 1268, 1408
AatII	GACGTC	2 cut(s)	755, 840
Acc36I	ACCTGC	1 cut(s)	1626
Acc65I	GGTACC	1 cut(s)	630
AccB1I	GGYRCC	4 cut(s)	227, 630, 962, 1690
AccB7I	CCANNNNNTGG	2 cut(s)	566, 959
AccBSI	CCGCTC	1 cut(s)	69
AccI	GTMKAC	1 cut(s)	840
AccII	CGCG	5 cut(s)	505, 710, 848, 1212, 1529
AclI	AACGTT	1 cut(s)	1361
AclWI	GGATC	5 cut(s)	580, 950, 1064, 1077, 1270
AcoI	YGGCCR	2 cut(s)	450, 1539
AcsI	RAATTY	1 cut(s)	735
AcvI	CACGTG	2 cut(s)	303, 1582
AcyI	GRCGYC	4 cut(s)	228, 752, 837, 963
AfaI	GTAC	7 cut(s)	531, 632, 725, 773, 881, 1141, 1259
AfiI	CCNNNNNNNGG	9 cut(s)	181, 392, 566, 959, 965, 1285, 1441, 1700, 1725
AflIII	ACRYGT	2 cut(s)	302, 1509
AgsI	TTSAA	2 cut(s)	380, 814
AjiI	CACGTC	1 cut(s)	1510
AjnI	CCWGG	3 cut(s)	420, 804, 958
AloI	GAACNNNNNNTCC	2 cut(s)	1160, 1192
AluBI	AGCT	6 cut(s)	664, 716, 802, 1430, 1638, 1741
AluI	AGCT	6 cut(s)	664, 716, 802, 1430, 1638, 1741
Alw21I	GWGCWC	2 cut(s)	666, 1743
Alw26I	GTCTC	4 cut(s)	242, 971, 1276, 1655
AlwI	GGATC	5 cut(s)	580, 950, 1064, 1077, 1270
AlwNI	CAGNNNCTG	1 cut(s)	959
Ama87I	CYCGRG	1 cut(s)	1247
AoxI	GGCC	7 cut(s)	184, 394, 410, 450, 1406, 1539, 1709
ApeKI	GCWGC	3 cut(s)	144, 416, 1430
ApoI	RAATTY	1 cut(s)	735
ArsI	GACNNNNNNTTYG	4 cut(s)	453, 485, 665, 697
AseI	ATTAAT	1 cut(s)	1746
Asp718I	GGTACC	1 cut(s)	630
AspLEI	GCGC	8 cut(s)	144, 230, 507, 712, 787, 941, 965, 1212
AspS9I	GGNCC	9 cut(s)	184, 330, 395, 411, 673, 825, 1047, 1102, 1709
AsuC2I	CCSGG	3 cut(s)	232, 1486, 1543
AsuHPI	GGTGA	6 cut(s)	173, 992, 1079, 1411, 1474, 1708
AsuII	TTCGAA	1 cut(s)	1292
AvaI	CYCGRG	1 cut(s)	1247
AvaII	GGWCC	5 cut(s)	330, 673, 825, 1047, 1102
BaeGI	GKGCMC	1 cut(s)	487
BalI	TGGCCA	1 cut(s)	452
BamHI	GGATCC	1 cut(s)	1069
BanI	GGYRCC	4 cut(s)	227, 630, 962, 1690
BanII	GRGCYC	2 cut(s)	666, 1743
BarI	GAAGNNNNNNTAC	2 cut(s)	623, 655
BbrPI	CACGTG	2 cut(s)	303, 1582
Bbv12I	GWGCWC	2 cut(s)	666, 1743
BbvI	GCAGC	3 cut(s)	131, 403, 1417
BccI	CCATC	6 cut(s)	344, 374, 520, 630, 883, 1727
BceAI	ACGGC	3 cut(s)	452, 1317, 1393
BciT130I	CCWGG	3 cut(s)	422, 806, 960
BciVI	GTATCC	1 cut(s)	548
BcnI	CCSGG	3 cut(s)	232, 1486, 1543
BcoDI	GTCTC	4 cut(s)	242, 971, 1276, 1655
BfaI	CTAG	3 cut(s)	176, 188, 408
BfoI	RGCGCY	2 cut(s)	231, 966
BfuAI	ACCTGC	1 cut(s)	1626
BfuI	GTATCC	1 cut(s)	548
BisI	GCNGC	7 cut(s)	145, 273, 417, 1062, 1431, 1586, 1622
BlpI	GCTNAGC	1 cut(s)	1625
BlsI	GCNGC	7 cut(s)	146, 274, 418, 1063, 1432, 1587, 1623
Bme1390I	CCNGG	6 cut(s)	232, 422, 806, 960, 1486, 1543
Bme18I	GGWCC	5 cut(s)	330, 673, 825, 1047, 1102
BmeT110I	CYCGRG	1 cut(s)	1247
BmgBI	CACGTC	1 cut(s)	1510
BmgT120I	GGNCC	9 cut(s)	184, 330, 395, 411, 673, 825, 1047, 1102, 1709
BmiI	GGNNCC	9 cut(s)	229, 331, 632, 674, 964, 1048, 1071, 1692, 1710
BmrFI	CCNGG	6 cut(s)	232, 422, 806, 960, 1486, 1543
BmrI	ACTGGG	1 cut(s)	712
BmsI	GCATC	3 cut(s)	468, 796, 1645
BmuI	ACTGGG	1 cut(s)	712
BplI	GAGNNNNNCTC	2 cut(s)	1256, 1288
Bpu10I	CCTNAGC	1 cut(s)	1446
Bpu1102I	GCTNAGC	1 cut(s)	1625
Bpu14I	TTCGAA	1 cut(s)	1292
BpuMI	CCSGG	3 cut(s)	232, 1486, 1543
Bsa29I	ATCGAT	1 cut(s)	339
BsaAI	YACGTR	2 cut(s)	303, 1582
BsaHI	GRCGYC	4 cut(s)	228, 752, 837, 963
BsaI	GGTCTC	2 cut(s)	242, 971
BsaJI	CCNNGG	3 cut(s)	231, 420, 1280
BsaWI	WCCGGW	1 cut(s)	1072
Bsc4I	CCNNNNNNNGG	9 cut(s)	181, 392, 566, 959, 965, 1285, 1441, 1700, 1725
Bse1I	ACTGG	2 cut(s)	707, 769
BseBI	CCWGG	3 cut(s)	422, 806, 960
BseCI	ATCGAT	1 cut(s)	339
BseDI	CCNNGG	3 cut(s)	231, 420, 1280
BseGI	GGATG	3 cut(s)	755, 1456, 1660
BseLI	CCNNNNNNNGG	9 cut(s)	181, 392, 566, 959, 965, 1285, 1441, 1700, 1725
BseMII	CTCAG	3 cut(s)	405, 1460, 1616
BseNI	ACTGG	2 cut(s)	707, 769
BseSI	GKGCMC	1 cut(s)	487
BseXI	GCAGC	3 cut(s)	131, 403, 1417
BseYI	CCCAGC	2 cut(s)	1023, 1565
BsgI	GTGCAG	1 cut(s)	26
Bsh1236I	CGCG	5 cut(s)	505, 710, 848, 1212, 1529
BshFI	GGCC	7 cut(s)	186, 396, 412, 452, 1408, 1541, 1711
BshNI	GGYRCC	4 cut(s)	227, 630, 962, 1690
BshVI	ATCGAT	1 cut(s)	339
BsiHKAI	GWGCWC	2 cut(s)	666, 1743
BsiHKCI	CYCGRG	1 cut(s)	1247
BsiSI	CCGG	5 cut(s)	231, 966, 1073, 1485, 1542
BslFI	GGGAC	7 cut(s)	76, 293, 686, 719, 959, 1060, 1238
BslI	CCNNNNNNNGG	9 cut(s)	181, 392, 566, 959, 965, 1285, 1441, 1700, 1725
BsmAI	GTCTC	4 cut(s)	242, 971, 1276, 1655
BsmFI	GGGAC	7 cut(s)	76, 293, 686, 719, 959, 1060, 1238
BsmI	GAATGC	1 cut(s)	904
BsnI	GGCC	7 cut(s)	186, 396, 412, 452, 1408, 1541, 1711
Bso31I	GGTCTC	2 cut(s)	242, 971
BsoBI	CYCGRG	1 cut(s)	1247
Bsp119I	TTCGAA	1 cut(s)	1292
Bsp1286I	GDGCHC	3 cut(s)	487, 666, 1743
Bsp1407I	TGTACA	2 cut(s)	723, 879
Bsp143I	GATC	6 cut(s)	178, 585, 955, 1069, 1275, 1348
Bsp1720I	GCTNAGC	1 cut(s)	1625
BspANI	GGCC	7 cut(s)	186, 396, 412, 452, 1408, 1541, 1711
BspCNI	CTCAG	3 cut(s)	404, 1459, 1617
BspDI	ATCGAT	1 cut(s)	339
BspFNI	CGCG	5 cut(s)	505, 710, 848, 1212, 1529
BspLI	GGNNCC	9 cut(s)	229, 331, 632, 674, 964, 1048, 1071, 1692, 1710
BspMI	ACCTGC	1 cut(s)	1626
BspPI	GGATC	5 cut(s)	580, 950, 1064, 1077, 1270
BspT104I	TTCGAA	1 cut(s)	1292
BspT107I	GGYRCC	4 cut(s)	227, 630, 962, 1690
BspTNI	GGTCTC	2 cut(s)	242, 971
BsrBI	CCGCTC	1 cut(s)	69
BsrGI	TGTACA	2 cut(s)	723, 879
BsrI	ACTGG	2 cut(s)	707, 769
BssECI	CCNNGG	3 cut(s)	231, 420, 1280
BssMI	GATC	6 cut(s)	178, 585, 955, 1069, 1275, 1348
BssNI	GRCGYC	4 cut(s)	228, 752, 837, 963
Bst2UI	CCWGG	3 cut(s)	422, 806, 960
Bst4CI	ACNGT	5 cut(s)	245, 551, 1336, 1498, 1673
Bst6I	CTCTTC	1 cut(s)	1641
BstACI	GRCGYC	4 cut(s)	228, 752, 837, 963
BstAUI	TGTACA	2 cut(s)	723, 879
BstBAI	YACGTR	2 cut(s)	303, 1582
BstBI	TTCGAA	1 cut(s)	1292
BstC8I	GCNNGC	8 cut(s)	316, 320, 414, 483, 662, 714, 718, 1539
BstDEI	CTNAG	4 cut(s)	391, 1413, 1446, 1625
BstDSI	CCRYGG	1 cut(s)	1280
BstENI	CCTNNNNNAGG	2 cut(s)	179, 390
BstF5I	GGATG	3 cut(s)	755, 1456, 1660
BstFNI	CGCG	5 cut(s)	505, 710, 848, 1212, 1529
BstH2I	RGCGCY	2 cut(s)	231, 966
BstHHI	GCGC	8 cut(s)	144, 230, 507, 712, 787, 941, 965, 1212
BstKTI	GATC	6 cut(s)	181, 588, 958, 1072, 1278, 1351
BstMAI	GTCTC	4 cut(s)	242, 971, 1276, 1655
BstMBI	GATC	6 cut(s)	178, 585, 955, 1069, 1275, 1348
BstMWI	GCNNNNNNNGC	7 cut(s)	272, 402, 521, 543, 799, 1591, 1635
BstNI	CCWGG	3 cut(s)	422, 806, 960
BstNSI	RCATGY	3 cut(s)	214, 724, 1111
BstSCI	CCNGG	6 cut(s)	230, 420, 804, 958, 1484, 1541
BstSLI	GKGCMC	1 cut(s)	487
BstUI	CGCG	5 cut(s)	505, 710, 848, 1212, 1529
BstV1I	GCAGC	3 cut(s)	131, 403, 1417
BstX2I	RGATCY	3 cut(s)	585, 955, 1069
BstYI	RGATCY	3 cut(s)	585, 955, 1069
Bsu15I	ATCGAT	1 cut(s)	339
BsuI	GTATCC	1 cut(s)	548
BsuRI	GGCC	7 cut(s)	186, 396, 412, 452, 1408, 1541, 1711
BsuTUI	ATCGAT	1 cut(s)	339
BtgI	CCRYGG	1 cut(s)	1280
BtgZI	GCGATG	1 cut(s)	539
BtrI	CACGTC	1 cut(s)	1510
BtsCI	GGATG	3 cut(s)	755, 1456, 1660
BtsI	GCAGTG	2 cut(s)	101, 532
BtsIMutI	CAGTG	3 cut(s)	101, 532, 741
BveI	ACCTGC	1 cut(s)	1626
Cac8I	GCNNGC	8 cut(s)	316, 320, 414, 483, 662, 714, 718, 1539
CaiI	CAGNNNCTG	1 cut(s)	959
CfoI	GCGC	8 cut(s)	144, 230, 507, 712, 787, 941, 965, 1212
Cfr13I	GGNCC	9 cut(s)	184, 330, 395, 411, 673, 825, 1047, 1102, 1709
ClaI	ATCGAT	1 cut(s)	339
CseI	GACGC	6 cut(s)	716, 854, 1178, 1201, 1381, 1535
Csp6I	GTAC	7 cut(s)	530, 631, 724, 772, 880, 1140, 1258
CviAII	CATG	6 cut(s)	211, 655, 721, 1042, 1108, 1207
CviQI	GTAC	7 cut(s)	530, 631, 724, 772, 880, 1140, 1258
DdeI	CTNAG	4 cut(s)	391, 1413, 1446, 1625
DinI	GGCGCC	2 cut(s)	229, 964
DpnI	GATC	6 cut(s)	180, 587, 957, 1071, 1277, 1350
DpnII	GATC	6 cut(s)	178, 585, 955, 1069, 1275, 1348
DrdI	GACNNNNNNGTC	3 cut(s)	836, 1268, 1408
DseDI	GACNNNNNNGTC	3 cut(s)	836, 1268, 1408
EaeI	YGGCCR	2 cut(s)	450, 1539
Eam1104I	CTCTTC	1 cut(s)	1641
EarI	CTCTTC	1 cut(s)	1641
EciI	GGCGGA	1 cut(s)	274
Ecl136II	GAGCTC	2 cut(s)	664, 1741
Eco24I	GRGCYC	2 cut(s)	666, 1743
Eco31I	GGTCTC	2 cut(s)	242, 971
Eco47I	GGWCC	5 cut(s)	330, 673, 825, 1047, 1102
Eco53kI	GAGCTC	2 cut(s)	664, 1741
Eco72I	CACGTG	2 cut(s)	303, 1582
Eco88I	CYCGRG	1 cut(s)	1247
EcoICRI	GAGCTC	2 cut(s)	664, 1741
EcoNI	CCTNNNNNAGG	2 cut(s)	179, 390
EcoO109I	RGGNCCY	2 cut(s)	184, 1047
EcoRII	CCWGG	3 cut(s)	420, 804, 958
EcoT38I	GRGCYC	2 cut(s)	666, 1743
EgeI	GGCGCC	2 cut(s)	229, 964
EheI	GGCGCC	2 cut(s)	229, 964
FaeI	CATG	6 cut(s)	214, 658, 724, 1045, 1111, 1210
FaqI	GGGAC	7 cut(s)	76, 293, 686, 719, 959, 1060, 1238
FatI	CATG	6 cut(s)	210, 654, 720, 1041, 1107, 1206
FauI	CCCGC	4 cut(s)	124, 421, 474, 510
FblI	GTMKAC	1 cut(s)	840
Fnu4HI	GCNGC	7 cut(s)	145, 273, 417, 1062, 1431, 1586, 1622
FokI	GGATG	3 cut(s)	742, 1463, 1667
FriOI	GRGCYC	2 cut(s)	666, 1743
Fsp4HI	GCNGC	7 cut(s)	145, 273, 417, 1062, 1431, 1586, 1622
FspBI	CTAG	3 cut(s)	176, 188, 408
GlaI	GCGC	8 cut(s)	143, 229, 506, 711, 786, 940, 964, 1211
GluI	GCNGC	7 cut(s)	145, 273, 417, 1062, 1431, 1586, 1622
GsaI	CCCAGC	2 cut(s)	1027, 1569
HaeII	RGCGCY	2 cut(s)	231, 966
HaeIII	GGCC	7 cut(s)	186, 396, 412, 452, 1408, 1541, 1711
HapII	CCGG	5 cut(s)	231, 966, 1073, 1485, 1542
HgaI	GACGC	6 cut(s)	716, 854, 1178, 1201, 1381, 1535
HhaI	GCGC	8 cut(s)	144, 230, 507, 712, 787, 941, 965, 1212
Hin1I	GRCGYC	4 cut(s)	228, 752, 837, 963
Hin1II	CATG	6 cut(s)	214, 658, 724, 1045, 1111, 1210
Hin6I	GCGC	8 cut(s)	142, 228, 505, 710, 785, 939, 963, 1210
HinP1I	GCGC	8 cut(s)	142, 228, 505, 710, 785, 939, 963, 1210
HincII	GTYRAC	2 cut(s)	841, 1314
HindII	GTYRAC	2 cut(s)	841, 1314
HindIII	AAGCTT	1 cut(s)	1636
HinfI	GANTC	8 cut(s)	97, 195, 389, 855, 1201, 1226, 1289, 1532
HpaII	CCGG	5 cut(s)	231, 966, 1073, 1485, 1542
HphI	GGTGA	6 cut(s)	173, 992, 1079, 1411, 1474, 1708
Hpy166II	GTNNAC	9 cut(s)	333, 532, 595, 841, 1102, 1258, 1314, 1519, 1579
Hpy188I	TCNGA	5 cut(s)	577, 1348, 1381, 1558, 1724
Hpy188III	TCNNGA	7 cut(s)	472, 491, 697, 731, 1223, 1273, 1448
Hpy8I	GTNNAC	9 cut(s)	333, 532, 595, 841, 1102, 1258, 1314, 1519, 1579
HpyAV	CCTTC	5 cut(s)	70, 691, 703, 1435, 1699
HpyCH4III	ACNGT	5 cut(s)	245, 551, 1336, 1498, 1673
HpyCH4IV	ACGT	8 cut(s)	302, 426, 752, 837, 1098, 1361, 1509, 1581
HpyCH4V	TGCA	4 cut(s)	43, 106, 147, 431
HpyF10VI	GCNNNNNNNGC	7 cut(s)	272, 402, 521, 543, 799, 1591, 1635
HpyF3I	CTNAG	4 cut(s)	391, 1413, 1446, 1625
HpySE526I	ACGT	8 cut(s)	302, 426, 752, 837, 1098, 1361, 1509, 1581
Hsp92I	GRCGYC	4 cut(s)	228, 752, 837, 963
Hsp92II	CATG	6 cut(s)	214, 658, 724, 1045, 1111, 1210
HspAI	GCGC	8 cut(s)	142, 228, 505, 710, 785, 939, 963, 1210
KasI	GGCGCC	2 cut(s)	227, 962
KpnI	GGTACC	1 cut(s)	634
Kzo9I	GATC	6 cut(s)	178, 585, 955, 1069, 1275, 1348
LmnI	GCTCC	1 cut(s)	807
Lsp1109I	GCAGC	3 cut(s)	131, 403, 1417
LweI	GCATC	3 cut(s)	468, 796, 1645
MaeI	CTAG	3 cut(s)	176, 188, 408
MaeII	ACGT	8 cut(s)	302, 426, 752, 837, 1098, 1361, 1509, 1581
MaeIII	GTNAC	4 cut(s)	161, 509, 1399, 1681
MalI	GATC	6 cut(s)	180, 587, 957, 1071, 1277, 1350
MbiI	CCGCTC	1 cut(s)	69
MboI	GATC	6 cut(s)	178, 585, 955, 1069, 1275, 1348
MboII	GAAGA	6 cut(s)	55, 604, 1335, 1450, 1612, 1658
MflI	RGATCY	3 cut(s)	585, 955, 1069
MhlI	GDGCHC	3 cut(s)	487, 666, 1743
MlsI	TGGCCA	1 cut(s)	452
MluCI	AATT	2 cut(s)	735, 814
MluNI	TGGCCA	1 cut(s)	452
Mly113I	GGCGCC	2 cut(s)	228, 963
MlyI	GAGTC	5 cut(s)	91, 189, 383, 1220, 1526
MmeI	TCCRAC	5 cut(s)	547, 1119, 1536, 1623, 1632
MnlI	CCTC	9 cut(s)	644, 698, 770, 1030, 1059, 1390, 1398, 1447, 1718
Mox20I	TGGCCA	1 cut(s)	452
MscI	TGGCCA	1 cut(s)	452
MseI	TTAA	3 cut(s)	1467, 1608, 1746
Msp20I	TGGCCA	1 cut(s)	452
MspA1I	CMGCKG	3 cut(s)	416, 716, 1430
MspI	CCGG	5 cut(s)	231, 966, 1073, 1485, 1542
MspR9I	CCNGG	6 cut(s)	232, 422, 806, 960, 1486, 1543
Mva1269I	GAATGC	1 cut(s)	904
MvaI	CCWGG	3 cut(s)	422, 806, 960
MvnI	CGCG	5 cut(s)	505, 710, 848, 1212, 1529
MwoI	GCNNNNNNNGC	7 cut(s)	272, 402, 521, 543, 799, 1591, 1635
NarI	GGCGCC	2 cut(s)	228, 963
NciI	CCSGG	3 cut(s)	232, 1486, 1543
NdeII	GATC	6 cut(s)	178, 585, 955, 1069, 1275, 1348
NlaIII	CATG	6 cut(s)	214, 658, 724, 1045, 1111, 1210
NlaIV	GGNNCC	9 cut(s)	229, 331, 632, 674, 964, 1048, 1071, 1692, 1710
NmuCI	GTSAC	4 cut(s)	161, 509, 1399, 1681
NspI	RCATGY	3 cut(s)	214, 724, 1111
NspV	TTCGAA	1 cut(s)	1292
PcsI	WCGNNNNNNNCGW	2 cut(s)	837, 1235
PctI	GAATGC	1 cut(s)	904
PfeI	GAWTC	3 cut(s)	855, 1201, 1289
PflMI	CCANNNNNTGG	2 cut(s)	566, 959
PfoI	TCCNGGA	1 cut(s)	804
PkrI	GCNGC	7 cut(s)	146, 274, 418, 1063, 1432, 1587, 1623
PleI	GAGTC	5 cut(s)	91, 189, 383, 1220, 1526
PluTI	GGCGCC	2 cut(s)	231, 966
PmaCI	CACGTG	2 cut(s)	303, 1582
PmlI	CACGTG	2 cut(s)	303, 1582
PpsI	GAGTC	5 cut(s)	91, 189, 383, 1220, 1526
Ppu21I	YACGTR	2 cut(s)	303, 1582
PpuMI	RGGWCCY	1 cut(s)	1047
PshBI	ATTAAT	1 cut(s)	1746
Psp124BI	GAGCTC	2 cut(s)	666, 1743
Psp1406I	AACGTT	1 cut(s)	1361
Psp5II	RGGWCCY	1 cut(s)	1047
Psp6I	CCWGG	3 cut(s)	420, 804, 958
PspCI	CACGTG	2 cut(s)	303, 1582
PspFI	CCCAGC	2 cut(s)	1023, 1565
PspGI	CCWGG	3 cut(s)	420, 804, 958
PspN4I	GGNNCC	9 cut(s)	229, 331, 632, 674, 964, 1048, 1071, 1692, 1710
PspPI	GGNCC	9 cut(s)	184, 330, 395, 411, 673, 825, 1047, 1102, 1709
PspPPI	RGGWCCY	1 cut(s)	1047
PstNI	CAGNNNCTG	1 cut(s)	959
PsuI	RGATCY	3 cut(s)	585, 955, 1069
PvuII	CAGCTG	2 cut(s)	716, 1430
RsaI	GTAC	7 cut(s)	531, 632, 725, 773, 881, 1141, 1259
RsaNI	GTAC	7 cut(s)	530, 631, 724, 772, 880, 1140, 1258
SacI	GAGCTC	2 cut(s)	666, 1743
SalI	GTCGAC	1 cut(s)	839
SaqAI	TTAA	3 cut(s)	1467, 1608, 1746
SatI	GCNGC	7 cut(s)	145, 273, 417, 1062, 1431, 1586, 1622
Sau3AI	GATC	6 cut(s)	178, 585, 955, 1069, 1275, 1348
Sau96I	GGNCC	9 cut(s)	184, 330, 395, 411, 673, 825, 1047, 1102, 1709
SchI	GAGTC	5 cut(s)	91, 189, 383, 1220, 1526
ScrFI	CCNGG	6 cut(s)	232, 422, 806, 960, 1486, 1543
SduI	GDGCHC	3 cut(s)	487, 666, 1743
SfaNI	GCATC	3 cut(s)	468, 796, 1645
SfoI	GGCGCC	2 cut(s)	229, 964
SfuI	TTCGAA	1 cut(s)	1292
SgrDI	CGTCGACG	1 cut(s)	839
SinI	GGWCC	5 cut(s)	330, 673, 825, 1047, 1102
Sse9I	AATT	2 cut(s)	735, 814
SspDI	GGCGCC	2 cut(s)	227, 962
SspI	AATATT	2 cut(s)	997, 1606
SspMI	CTAG	3 cut(s)	176, 188, 408
SstI	GAGCTC	2 cut(s)	666, 1743
StyD4I	CCNGG	6 cut(s)	230, 420, 804, 958, 1484, 1541
TaaI	ACNGT	5 cut(s)	245, 551, 1336, 1498, 1673
TaiI	ACGT	8 cut(s)	305, 429, 755, 840, 1101, 1364, 1512, 1584
TaqII	GACCGA	1 cut(s)	842
TasI	AATT	2 cut(s)	735, 814
TatI	WGTACW	3 cut(s)	723, 771, 879
TauI	GCSGC	4 cut(s)	275, 1064, 1588, 1624
TfiI	GAWTC	3 cut(s)	855, 1201, 1289
Tru1I	TTAA	3 cut(s)	1467, 1608, 1746
Tru9I	TTAA	3 cut(s)	1467, 1608, 1746
TscAI	CASTG	3 cut(s)	108, 539, 748
TseFI	GTSAC	4 cut(s)	161, 509, 1399, 1681
TseI	GCWGC	3 cut(s)	144, 416, 1430
Tsp45I	GTSAC	4 cut(s)	161, 509, 1399, 1681
TspDTI	ATGAA	2 cut(s)	1329, 1506
TspGWI	ACGGA	3 cut(s)	939, 1157, 1438
TspRI	CASTG	3 cut(s)	108, 539, 748
Van91I	CCANNNNNTGG	2 cut(s)	566, 959
VpaK11BI	GGWCC	5 cut(s)	330, 673, 825, 1047, 1102
VspI	ATTAAT	1 cut(s)	1746
XagI	CCTNNNNNAGG	2 cut(s)	179, 390
XapI	RAATTY	1 cut(s)	735
XceI	RCATGY	3 cut(s)	214, 724, 1111
XmiI	GTMKAC	1 cut(s)	840
XspI	CTAG	3 cut(s)	176, 188, 408
ZraI	GACGTC	2 cut(s)	753, 838

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.