Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0006919

Physical Location & Seq

Genomic Coordinates Reverse (-)

5524546 .. 5526303

Gene Structure 1758 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031479

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGTCTTCTCCTCCTCTACCACCCACTGCTACAATCATTTGCCCCAACTCCACTTCACTAACCTCCATCACTTCTTTCACTCCCTTCTTCCCCAAGAAATCCCAAAAGTCTTCCTTAATTACAAAACCGAAGCAAGGCTTTGTATGCAAGGCAACAAACAACAATGACCAAAATCAAGATGCTATGGGAAAATTGGACAGGAGAAACATGCTCATCGGTTTGGGTGCTGGCGGCCTATACGGCACGGTGGGTCTTGAAACCAATCCGTTTGCATTTGCCGCACCTGTGCCACCGCCGGACTTAGCCACGTGCGGTCCGGCGGACAAGCCTGATGGTTCAACTATCGATTGCTGCCCGCCAAAAACCACCACAATCATTGACTTCAAGCTACCCGACCCGGGTCCATTGCGCACCAGGCTCGCTTGTCAGGATGTCGCAAAAGACCCCGCATACTTGGCAAAATATAAAAAGGCCGTCGAGCTCATGCGGGCCCTTCCCGAAGACGACCCACGAAGCCTGGCCCAGCAGGCCATGGTTCACTGTTCGTATTGCGACGGTGGGTATCCAATGCCCGGGTTTTCCGACCTCGAAATCCAAGTCCACTTCTGTTGGCTCTTCTTCCCTTTCCACCGTTGGTATCTCTACTTCTACGAGAAGATCATGGGCAAGCTCATCGACGACCCCACTTTCGCTCTTCCATTCTGGAACTGGGACGCTCCGGCCGGTATGTACTTGCCGTCTATTTTTACCGACACCACCTCCTCTCTGTACGACCAATACCGAAACGCAGCGCATCAACCCCCGAAGCTCCTAGACCTCAATTATGATGGCACCGACGATGACACCGATGACAAGACTAGAATCAAGGAGAACCTCACGACCATGTACCAACAAATGGTCTCTAAGGCCACTTCTCAGCGCCTCTTCTTCGGTGAGCCCTACACTGCCGGCGACGACCCGAACCCCGGCGCCGGAAACATCGAGACTATTCCACATAACAACATCCATCTCTGGTCGGGAGATCCTACTCAGACTAATGGGGAGGACATGGGAGCTTTCTACTCAGCAGGGAGGGATCCTCTGTTTTACTCTCACCATGCCAACGTAGACCGCATGTGGTCTATCTACAAAGCGCGAGGTGGGACTGATATCACCAAAACTGATTATCTTAACACCGAGTTTTTGTTCTACGACGAGAACAAGAATCTGGTCAGAGTCAAAGTCCGTGACTCGCTGGACGAGTCGAAACTCGGGTACAAGTACCAGGACGTGGAGATCCCGTGGCTGGAGTCGAAGCCTACGGCAAGGAAGTCGAAGAATAAAAGGAAGGCGGCGGTTTCCTCCGCGGATTTGACTTCGAAGTTTCCGGCGACGTTGAGTGAGACTATTAGTGTTGAGGTGGCGCGGCCTTCGGTGGCGAAGAGGACAAAGGCTGAGAAGGCGAAGGAGGAGGAGGTGTTGGTGATCAGCGGTATCGAGTTTGCCGGAGGCGAGAAGTTGAAGTTTGATGTGTATGTGAATGATGATGCGGATGTGCTGAGCGGGAAGGACAAGGCGGAGTTTGCCGGAAGTTTTGTGCACGTGCCGCATAGGGTGAAGAAGAAGATCAAGACGAACTTGAGGTTGGCGATAACGAATTTGCTGGCGGATTTGGGGGCGGAGGATGATAGTAGTGTGGTGGTGACTTTGGTGCCGAAGTTTGGGAAAGGGCCTATCACCATTGGTGGGTTCAAGATCGAGCTTATTACTACCACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

586

Amino Acids

64.85

Weight (kDa)

6.12

Isoelectric Point (pI)

38.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	172 - 378	2.4e-34	Common central domain of tyrosinase
PPO1_DWL	PF12142	383 - 434	6.2e-26	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	455 - 581	4.6e-48	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g04680
malus_domestica	MD05G1319800.v1.1 MD05G1320100.v1.1 MD05G1320200.v1.1 MD05G1320500.v1.1 MD05G1320800.v1.1 MD10G1298300.v1.1
prunus_persica	Prupe.4G041500_v2.0.a1 Prupe.4G041700_v2.0.a1
pyrus_communis	pycom05g29530 pycom05g29560 pycom10g24890 pycom10g24910 pycom10g25140
rosa_chinensis	RchiOBHm_Chr5g0007621
rosa_laevigata	RLG00000031479
rosa_multiflora	Rmu_co8439317.1_g000001 Rmu_sc0011744.1_g000006 Rmu_sc0021005.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068730
rosa_rugosa	Rorug04G0429400
rosa_samantha	Rh5AG057500 Rh5BG054400 Rh5CG064400 Rh5DG053600
rosa_wichuraiana	Rw5G006180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	410
AccB1I	GGYRCC	3 cut(s)	830, 968, 1690
AccB7I	CCANNNNNTGG	2 cut(s)	895, 1270
AccBSI	CCGCTC	1 cut(s)	1542
AccI	GTMKAC	1 cut(s)	1107
AccII	CGCG	3 cut(s)	1135, 1346, 1405
AclWI	GGATC	4 cut(s)	1016, 1070, 1083, 1270
AcoI	YGGCCR	1 cut(s)	720
AcsI	RAATTY	1 cut(s)	1636
AcvI	CACGTG	2 cut(s)	309, 1582
AcyI	GRCGYC	1 cut(s)	969
AfaI	GTAC	5 cut(s)	731, 770, 887, 1256, 1262
AgsI	TTSAA	5 cut(s)	257, 339, 385, 1501, 1732
AjiI	CACGTC	1 cut(s)	1270
AjnI	CCWGG	3 cut(s)	413, 516, 1263
AjuI	GAANNNNNNNTTGG	2 cut(s)	1607, 1639
AluBI	AGCT	6 cut(s)	388, 481, 670, 808, 1055, 1741
AluI	AGCT	6 cut(s)	388, 481, 670, 808, 1055, 1741
Alw21I	GWGCWC	2 cut(s)	483, 1581
Alw26I	GTCTC	3 cut(s)	904, 977, 1376
Alw44I	GTGCAC	1 cut(s)	1577
AlwI	GGATC	4 cut(s)	1016, 1070, 1083, 1270
Ama87I	CYCGRG	3 cut(s)	397, 572, 1250
AoxI	GGCC	9 cut(s)	232, 471, 489, 519, 528, 720, 906, 1406, 1709
ApaI	GGGCCC	1 cut(s)	493
ApaLI	GTGCAC	1 cut(s)	1577
ApeKI	GCWGC	2 cut(s)	351, 788
ApoI	RAATTY	1 cut(s)	1636
ArsI	GACNNNNNNTTYG	4 cut(s)	582, 614, 671, 703
AspLEI	GCGC	6 cut(s)	411, 793, 921, 971, 1135, 1405
AspS9I	GGNCC	6 cut(s)	314, 401, 489, 490, 520, 1709
AsuC2I	CCSGG	5 cut(s)	398, 399, 573, 574, 966
AsuHPI	GGTGA	7 cut(s)	944, 1085, 1144, 1474, 1606, 1693, 1708
AsuII	TTCGAA	1 cut(s)	1358
AvaI	CYCGRG	3 cut(s)	397, 572, 1250
AvaII	GGWCC	2 cut(s)	314, 401
BaeGI	GKGCMC	2 cut(s)	493, 1581
BamHI	GGATCC	1 cut(s)	1075
BanI	GGYRCC	3 cut(s)	830, 968, 1690
BanII	GRGCYC	3 cut(s)	483, 493, 939
BarI	GAAGNNNNNNTAC	2 cut(s)	629, 661
BbrPI	CACGTG	2 cut(s)	309, 1582
BbsI	GAAGAC	2 cut(s)	102, 507
Bbv12I	GWGCWC	2 cut(s)	483, 1581
BbvI	GCAGC	2 cut(s)	338, 800
BccI	CCATC	4 cut(s)	74, 326, 821, 1014
BceAI	ACGGC	4 cut(s)	256, 458, 721, 1317
BcgI	CGANNNNNNTGC	6 cut(s)	400, 434, 655, 689, 1293, 1327
BciT130I	CCWGG	3 cut(s)	415, 518, 1265
BciVI	GTATCC	1 cut(s)	573
BclI	TGATCA	1 cut(s)	1464
BcnI	CCSGG	5 cut(s)	398, 399, 573, 574, 966
BcoDI	GTCTC	3 cut(s)	904, 977, 1376
BfaI	CTAG	2 cut(s)	812, 858
BfoI	RGCGCY	2 cut(s)	922, 972
BfuI	GTATCC	1 cut(s)	573
BglI	GCCNNNNNGGC	2 cut(s)	240, 527
BisI	GCNGC	7 cut(s)	232, 279, 352, 789, 1332, 1406, 1586
BlpI	GCTNAGC	1 cut(s)	1538
BlsI	GCNGC	7 cut(s)	233, 280, 353, 790, 1333, 1407, 1587
Bme1390I	CCNGG	8 cut(s)	398, 399, 415, 518, 573, 574, 966, 1265
Bme18I	GGWCC	2 cut(s)	314, 401
BmeT110I	CYCGRG	3 cut(s)	397, 572, 1250
BmgBI	CACGTC	1 cut(s)	1270
BmgT120I	GGNCC	6 cut(s)	314, 401, 489, 490, 520, 1709
BmiI	GGNNCC	6 cut(s)	402, 491, 832, 970, 1077, 1692
BmrFI	CCNGG	8 cut(s)	398, 399, 415, 518, 573, 574, 966, 1265
BmrI	ACTGGG	1 cut(s)	718
BmsI	GCATC	3 cut(s)	169, 802, 1516
BmuI	ACTGGG	1 cut(s)	718
BoxI	GACNNNNGTC	1 cut(s)	399
BpiI	GAAGAC	2 cut(s)	102, 507
BplI	GAGNNNNNCTC	2 cut(s)	1063, 1095
BpmI	CTGGAG	1 cut(s)	1307
Bpu1102I	GCTNAGC	1 cut(s)	1538
Bpu14I	TTCGAA	1 cut(s)	1358
BpuEI	CTTGAG	1 cut(s)	1639
BpuMI	CCSGG	5 cut(s)	398, 399, 573, 574, 966
Bsa29I	ATCGAT	1 cut(s)	345
BsaAI	YACGTR	2 cut(s)	309, 1582
BsaHI	GRCGYC	1 cut(s)	969
BsaI	GGTCTC	1 cut(s)	904
BsaJI	CCNNGG	6 cut(s)	397, 531, 572, 964, 1280, 1344
BsaXI	ACNNNNNCTCC	6 cut(s)	743, 773, 1265, 1295, 1442, 1472
Bse118I	RCCGGY	2 cut(s)	722, 947
Bse1I	ACTGG	1 cut(s)	713
Bse3DI	GCAATG	1 cut(s)	404
BseBI	CCWGG	3 cut(s)	415, 518, 1265
BseCI	ATCGAT	1 cut(s)	345
BseDI	CCNNGG	6 cut(s)	397, 531, 572, 964, 1280, 1344
BseGI	GGATG	4 cut(s)	436, 1002, 1537, 1669
BseMI	GCAATG	1 cut(s)	404
BseMII	CTCAG	5 cut(s)	929, 1043, 1077, 1425, 1529
BseNI	ACTGG	1 cut(s)	713
BseRI	GAGGAG	3 cut(s)	751, 1463, 1466
BseSI	GKGCMC	2 cut(s)	493, 1581
BseX3I	CGGCCG	1 cut(s)	720
BseXI	GCAGC	2 cut(s)	338, 800
BseYI	CCCAGC	1 cut(s)	522
Bsh1236I	CGCG	3 cut(s)	1135, 1346, 1405
Bsh1285I	CGRYCG	1 cut(s)	723
BshFI	GGCC	9 cut(s)	234, 473, 491, 521, 530, 722, 908, 1408, 1711
BshNI	GGYRCC	3 cut(s)	830, 968, 1690
BshVI	ATCGAT	1 cut(s)	345
BsiEI	CGRYCG	1 cut(s)	723
BsiHKAI	GWGCWC	2 cut(s)	483, 1581
BsiHKCI	CYCGRG	3 cut(s)	397, 572, 1250
BslFI	GGGAC	2 cut(s)	725, 1156
BsmAI	GTCTC	3 cut(s)	904, 977, 1376
BsmFI	GGGAC	2 cut(s)	725, 1156
BsnI	GGCC	9 cut(s)	234, 473, 491, 521, 530, 722, 908, 1408, 1711
Bso31I	GGTCTC	1 cut(s)	904
BsoBI	CYCGRG	3 cut(s)	397, 572, 1250
Bsp119I	TTCGAA	1 cut(s)	1358
Bsp120I	GGGCCC	1 cut(s)	489
Bsp1286I	GDGCHC	4 cut(s)	483, 493, 939, 1581
Bsp143I	GATC	7 cut(s)	657, 1021, 1075, 1275, 1464, 1605, 1734
Bsp1720I	GCTNAGC	1 cut(s)	1538
Bsp19I	CCATGG	1 cut(s)	531
BspANI	GGCC	9 cut(s)	234, 473, 491, 521, 530, 722, 908, 1408, 1711
BspCNI	CTCAG	5 cut(s)	928, 1042, 1076, 1426, 1530
BspDI	ATCGAT	1 cut(s)	345
BspFNI	CGCG	3 cut(s)	1135, 1346, 1405
BspLI	GGNNCC	6 cut(s)	402, 491, 832, 970, 1077, 1692
BspPI	GGATC	4 cut(s)	1016, 1070, 1083, 1270
BspQI	GCTCTTC	2 cut(s)	620, 699
BspT104I	TTCGAA	1 cut(s)	1358
BspT107I	GGYRCC	3 cut(s)	830, 968, 1690
BspTNI	GGTCTC	1 cut(s)	904
BsrBI	CCGCTC	1 cut(s)	1542
BsrDI	GCAATG	1 cut(s)	404
BsrFI	RCCGGY	2 cut(s)	722, 947
BsrI	ACTGG	1 cut(s)	713
BssAI	RCCGGY	2 cut(s)	722, 947
BssECI	CCNNGG	6 cut(s)	397, 531, 572, 964, 1280, 1344
BssMI	GATC	7 cut(s)	657, 1021, 1075, 1275, 1464, 1605, 1734
BssNI	GRCGYC	1 cut(s)	969
BssT1I	CCWWGG	1 cut(s)	531
Bst2UI	CCWGG	3 cut(s)	415, 518, 1265
Bst4CI	ACNGT	4 cut(s)	247, 542, 557, 632
Bst6I	CTCTTC	4 cut(s)	620, 699, 929, 1415
BstACI	GRCGYC	1 cut(s)	969
BstBAI	YACGTR	2 cut(s)	309, 1582
BstBI	TTCGAA	1 cut(s)	1358
BstC8I	GCNNGC	8 cut(s)	229, 356, 420, 489, 528, 668, 949, 1644
BstDEI	CTNAG	7 cut(s)	301, 903, 915, 1029, 1063, 1434, 1538
BstDSI	CCRYGG	3 cut(s)	531, 1280, 1344
BstF5I	GGATG	4 cut(s)	436, 1002, 1537, 1669
BstFNI	CGCG	3 cut(s)	1135, 1346, 1405
BstH2I	RGCGCY	2 cut(s)	922, 972
BstHHI	GCGC	6 cut(s)	411, 793, 921, 971, 1135, 1405
BstKTI	GATC	7 cut(s)	660, 1024, 1078, 1278, 1467, 1608, 1737
BstMAI	GTCTC	3 cut(s)	904, 977, 1376
BstMBI	GATC	7 cut(s)	657, 1021, 1075, 1275, 1464, 1605, 1734
BstMCI	CGRYCG	1 cut(s)	723
BstMWI	GCNNNNNNNGC	9 cut(s)	144, 240, 278, 415, 455, 527, 1439, 1562, 1585
BstNI	CCWGG	3 cut(s)	415, 518, 1265
BstNSI	RCATGY	2 cut(s)	211, 1117
BstPAI	GACNNNNGTC	1 cut(s)	399
BstSCI	CCNGG	8 cut(s)	396, 397, 413, 516, 571, 572, 964, 1263
BstSLI	GKGCMC	2 cut(s)	493, 1581
BstUI	CGCG	3 cut(s)	1135, 1346, 1405
BstV1I	GCAGC	2 cut(s)	338, 800
BstV2I	GAAGAC	2 cut(s)	102, 507
BstX2I	RGATCY	3 cut(s)	1021, 1075, 1275
BstYI	RGATCY	3 cut(s)	1021, 1075, 1275
BstZI	CGGCCG	1 cut(s)	720
Bsu15I	ATCGAT	1 cut(s)	345
BsuI	GTATCC	1 cut(s)	573
BsuRI	GGCC	9 cut(s)	234, 473, 491, 521, 530, 722, 908, 1408, 1711
BsuTUI	ATCGAT	1 cut(s)	345
BtgI	CCRYGG	3 cut(s)	531, 1280, 1344
BtrI	CACGTC	1 cut(s)	1270
BtsCI	GGATG	4 cut(s)	436, 1002, 1537, 1669
BtsI	GCAGTG	2 cut(s)	24, 942
BtsIMutI	CAGTG	3 cut(s)	24, 538, 942
Cac8I	GCNNGC	8 cut(s)	229, 356, 420, 489, 528, 668, 949, 1644
CfoI	GCGC	6 cut(s)	411, 793, 921, 971, 1135, 1405
Cfr10I	RCCGGY	2 cut(s)	722, 947
Cfr13I	GGNCC	6 cut(s)	314, 401, 489, 490, 520, 1709
Cfr42I	CCGCGG	1 cut(s)	1347
Cfr9I	CCCGGG	2 cut(s)	397, 572
ClaI	ATCGAT	1 cut(s)	345
CpoI	CGGWCCG	1 cut(s)	314
CseI	GACGC	1 cut(s)	722
Csp6I	GTAC	5 cut(s)	730, 769, 886, 1255, 1261
CspCI	CAANNNNNGTGG	4 cut(s)	358, 393, 590, 625
CspI	CGGWCCG	1 cut(s)	314
CviAII	CATG	8 cut(s)	208, 484, 532, 661, 883, 1048, 1097, 1114
CviQI	GTAC	5 cut(s)	730, 769, 886, 1255, 1261
DdeI	CTNAG	7 cut(s)	301, 903, 915, 1029, 1063, 1434, 1538
DinI	GGCGCC	1 cut(s)	970
DpnI	GATC	7 cut(s)	659, 1023, 1077, 1277, 1466, 1607, 1736
DpnII	GATC	7 cut(s)	657, 1021, 1075, 1275, 1464, 1605, 1734
EaeI	YGGCCR	1 cut(s)	720
EagI	CGGCCG	1 cut(s)	720
Eam1104I	CTCTTC	4 cut(s)	620, 699, 929, 1415
EarI	CTCTTC	4 cut(s)	620, 699, 929, 1415
EciI	GGCGGA	4 cut(s)	335, 1571, 1661, 1673
Ecl136II	GAGCTC	1 cut(s)	481
EclXI	CGGCCG	1 cut(s)	720
Eco130I	CCWWGG	1 cut(s)	531
Eco24I	GRGCYC	3 cut(s)	483, 493, 939
Eco31I	GGTCTC	1 cut(s)	904
Eco32I	GATATC	1 cut(s)	1150
Eco47I	GGWCC	2 cut(s)	314, 401
Eco52I	CGGCCG	1 cut(s)	720
Eco53kI	GAGCTC	1 cut(s)	481
Eco72I	CACGTG	2 cut(s)	309, 1582
Eco88I	CYCGRG	3 cut(s)	397, 572, 1250
EcoICRI	GAGCTC	1 cut(s)	481
EcoO109I	RGGNCCY	2 cut(s)	490, 1709
EcoRII	CCWGG	3 cut(s)	413, 516, 1263
EcoRV	GATATC	1 cut(s)	1150
EcoT14I	CCWWGG	1 cut(s)	531
EcoT38I	GRGCYC	3 cut(s)	483, 493, 939
EgeI	GGCGCC	1 cut(s)	970
EheI	GGCGCC	1 cut(s)	970
ErhI	CCWWGG	1 cut(s)	531
FaeI	CATG	8 cut(s)	211, 487, 535, 664, 886, 1051, 1100, 1117
FalI	AAGNNNNNCTT	6 cut(s)	98, 130, 122, 154, 1601, 1633
FaqI	GGGAC	2 cut(s)	725, 1156
FatI	CATG	8 cut(s)	207, 483, 531, 660, 882, 1047, 1096, 1113
FauI	CCCGC	4 cut(s)	363, 454, 480, 1535
FbaI	TGATCA	1 cut(s)	1464
FblI	GTMKAC	1 cut(s)	1107
Fnu4HI	GCNGC	7 cut(s)	232, 279, 352, 789, 1332, 1406, 1586
FokI	GGATG	4 cut(s)	443, 989, 1544, 1676
FriOI	GRGCYC	3 cut(s)	483, 493, 939
Fsp4HI	GCNGC	7 cut(s)	232, 279, 352, 789, 1332, 1406, 1586
FspBI	CTAG	2 cut(s)	812, 858
FspI	TGCGCA	1 cut(s)	410
GlaI	GCGC	6 cut(s)	410, 792, 920, 970, 1134, 1404
GluI	GCNGC	7 cut(s)	232, 279, 352, 789, 1332, 1406, 1586
GsaI	CCCAGC	1 cut(s)	526
GsuI	CTGGAG	1 cut(s)	1307
HaeII	RGCGCY	2 cut(s)	922, 972
HaeIII	GGCC	9 cut(s)	234, 473, 491, 521, 530, 722, 908, 1408, 1711
HgaI	GACGC	1 cut(s)	722
HhaI	GCGC	6 cut(s)	411, 793, 921, 971, 1135, 1405
Hin1I	GRCGYC	1 cut(s)	969
Hin1II	CATG	8 cut(s)	211, 487, 535, 664, 886, 1051, 1100, 1117
Hin6I	GCGC	6 cut(s)	409, 791, 919, 969, 1133, 1403
HinP1I	GCGC	6 cut(s)	409, 791, 919, 969, 1133, 1403
HinfI	GANTC	6 cut(s)	861, 1204, 1215, 1229, 1241, 1289
HphI	GGTGA	7 cut(s)	944, 1085, 1144, 1474, 1606, 1693, 1708
Hpy166II	GTNNAC	4 cut(s)	538, 601, 1108, 1579
Hpy188I	TCNGA	3 cut(s)	583, 1032, 1214
Hpy8I	GTNNAC	4 cut(s)	538, 601, 1108, 1579
Hpy99I	CGWCG	7 cut(s)	479, 557, 680, 839, 956, 1196, 1375
HpyAV	CCTTC	7 cut(s)	94, 503, 1321, 1419, 1432, 1438, 1540
HpyCH4III	ACNGT	4 cut(s)	247, 542, 557, 632
HpyCH4IV	ACGT	5 cut(s)	308, 1104, 1269, 1373, 1581
HpyCH4V	TGCA	3 cut(s)	147, 272, 1579
HpyF10VI	GCNNNNNNNGC	9 cut(s)	144, 240, 278, 415, 455, 527, 1439, 1562, 1585
HpyF3I	CTNAG	7 cut(s)	301, 903, 915, 1029, 1063, 1434, 1538
HpySE526I	ACGT	5 cut(s)	308, 1104, 1269, 1373, 1581
Hsp92I	GRCGYC	1 cut(s)	969
Hsp92II	CATG	8 cut(s)	211, 487, 535, 664, 886, 1051, 1100, 1117
HspAI	GCGC	6 cut(s)	409, 791, 919, 969, 1133, 1403
KasI	GGCGCC	1 cut(s)	968
KroI	GCCGGC	1 cut(s)	947
KroNI	GCCGGC	1 cut(s)	949
Ksp22I	TGATCA	1 cut(s)	1464
KspI	CCGCGG	1 cut(s)	1347
Kzo9I	GATC	7 cut(s)	657, 1021, 1075, 1275, 1464, 1605, 1734
LguI	GCTCTTC	2 cut(s)	620, 699
LmnI	GCTCC	3 cut(s)	721, 813, 1052
Lsp1109I	GCAGC	2 cut(s)	338, 800
LweI	GCATC	3 cut(s)	169, 802, 1516
MaeI	CTAG	2 cut(s)	812, 858
MaeII	ACGT	5 cut(s)	308, 1104, 1269, 1373, 1581
MaeIII	GTNAC	2 cut(s)	1226, 1681
MalI	GATC	7 cut(s)	659, 1023, 1077, 1277, 1466, 1607, 1736
MbiI	CCGCTC	1 cut(s)	1542
MboI	GATC	7 cut(s)	657, 1021, 1075, 1275, 1464, 1605, 1734
MflI	RGATCY	3 cut(s)	1021, 1075, 1275
MhlI	GDGCHC	4 cut(s)	483, 493, 939, 1581
MluCI	AATT	4 cut(s)	117, 191, 820, 1636
Mly113I	GGCGCC	1 cut(s)	969
MlyI	GAGTC	4 cut(s)	1223, 1224, 1250, 1298
MmeI	TCCRAC	1 cut(s)	606
MroNI	GCCGGC	1 cut(s)	947
MseI	TTAA	2 cut(s)	116, 1170
MslI	CAYNNNNRTG	1 cut(s)	881
MspA1I	CMGCKG	2 cut(s)	1346, 1470
MspR9I	CCNGG	8 cut(s)	398, 399, 415, 518, 573, 574, 966, 1265
MvaI	CCWGG	3 cut(s)	415, 518, 1265
MvnI	CGCG	3 cut(s)	1135, 1346, 1405
MwoI	GCNNNNNNNGC	9 cut(s)	144, 240, 278, 415, 455, 527, 1439, 1562, 1585
NaeI	GCCGGC	1 cut(s)	949
NarI	GGCGCC	1 cut(s)	969
NciI	CCSGG	5 cut(s)	398, 399, 573, 574, 966
NcoI	CCATGG	1 cut(s)	531
NdeII	GATC	7 cut(s)	657, 1021, 1075, 1275, 1464, 1605, 1734
NgoMIV	GCCGGC	1 cut(s)	947
NlaIII	CATG	8 cut(s)	211, 487, 535, 664, 886, 1051, 1100, 1117
NlaIV	GGNNCC	6 cut(s)	402, 491, 832, 970, 1077, 1692
NmuCI	GTSAC	2 cut(s)	1226, 1681
NsbI	TGCGCA	1 cut(s)	410
NspI	RCATGY	2 cut(s)	211, 1117
NspV	TTCGAA	1 cut(s)	1358
PciSI	GCTCTTC	2 cut(s)	620, 699
PcsI	WCGNNNNNNNCGW	1 cut(s)	843
PdiI	GCCGGC	1 cut(s)	949
PfeI	GAWTC	2 cut(s)	861, 1204
PflMI	CCANNNNNTGG	2 cut(s)	895, 1270
PkrI	GCNGC	7 cut(s)	233, 280, 353, 790, 1333, 1407, 1587
PleI	GAGTC	4 cut(s)	1223, 1223, 1249, 1297
PluTI	GGCGCC	1 cut(s)	972
PmaCI	CACGTG	2 cut(s)	309, 1582
PmlI	CACGTG	2 cut(s)	309, 1582
PpsI	GAGTC	4 cut(s)	1223, 1223, 1249, 1297
Ppu21I	YACGTR	2 cut(s)	309, 1582
PshAI	GACNNNNGTC	1 cut(s)	399
Psp124BI	GAGCTC	1 cut(s)	483
Psp6I	CCWGG	3 cut(s)	413, 516, 1263
PspCI	CACGTG	2 cut(s)	309, 1582
PspFI	CCCAGC	1 cut(s)	522
PspGI	CCWGG	3 cut(s)	413, 516, 1263
PspN4I	GGNNCC	6 cut(s)	402, 491, 832, 970, 1077, 1692
PspOMI	GGGCCC	1 cut(s)	489
PspPI	GGNCC	6 cut(s)	314, 401, 489, 490, 520, 1709
PsuI	RGATCY	3 cut(s)	1021, 1075, 1275
RsaI	GTAC	5 cut(s)	731, 770, 887, 1256, 1262
RsaNI	GTAC	5 cut(s)	730, 769, 886, 1255, 1261
RseI	CAYNNNNRTG	1 cut(s)	881
Rsr2I	CGGWCCG	1 cut(s)	314
RsrII	CGGWCCG	1 cut(s)	314
SacI	GAGCTC	1 cut(s)	483
SacII	CCGCGG	1 cut(s)	1347
SapI	GCTCTTC	2 cut(s)	620, 699
SaqAI	TTAA	2 cut(s)	116, 1170
SatI	GCNGC	7 cut(s)	232, 279, 352, 789, 1332, 1406, 1586
Sau3AI	GATC	7 cut(s)	657, 1021, 1075, 1275, 1464, 1605, 1734
Sau96I	GGNCC	6 cut(s)	314, 401, 489, 490, 520, 1709
SchI	GAGTC	4 cut(s)	1223, 1224, 1250, 1298
ScrFI	CCNGG	8 cut(s)	398, 399, 415, 518, 573, 574, 966, 1265
SduI	GDGCHC	4 cut(s)	483, 493, 939, 1581
SfaNI	GCATC	3 cut(s)	169, 802, 1516
SfiI	GGCCNNNNNGGCC	1 cut(s)	527
SfoI	GGCGCC	1 cut(s)	970
Sfr303I	CCGCGG	1 cut(s)	1347
SfuI	TTCGAA	1 cut(s)	1358
SgrBI	CCGCGG	1 cut(s)	1347
SinI	GGWCC	2 cut(s)	314, 401
SmaI	CCCGGG	2 cut(s)	399, 574
SmiMI	CAYNNNNRTG	1 cut(s)	881
SmlI	CTYRAG	1 cut(s)	1618
SmoI	CTYRAG	1 cut(s)	1618
Sse9I	AATT	4 cut(s)	117, 191, 820, 1636
SspDI	GGCGCC	1 cut(s)	968
SspMI	CTAG	2 cut(s)	812, 858
SstI	GAGCTC	1 cut(s)	483
StyD4I	CCNGG	8 cut(s)	396, 397, 413, 516, 571, 572, 964, 1263
StyI	CCWWGG	1 cut(s)	531
TaaI	ACNGT	4 cut(s)	247, 542, 557, 632
TaiI	ACGT	5 cut(s)	311, 1107, 1272, 1376, 1584
TasI	AATT	4 cut(s)	117, 191, 820, 1636
TatI	WGTACW	1 cut(s)	729
TauI	GCSGC	5 cut(s)	234, 281, 1334, 1408, 1588
TfiI	GAWTC	2 cut(s)	861, 1204
Tru1I	TTAA	2 cut(s)	116, 1170
Tru9I	TTAA	2 cut(s)	116, 1170
TscAI	CASTG	3 cut(s)	31, 545, 949
TseFI	GTSAC	2 cut(s)	1226, 1681
TseI	GCWGC	2 cut(s)	351, 788
Tsp45I	GTSAC	2 cut(s)	1226, 1681
TspGWI	ACGGA	2 cut(s)	255, 1214
TspMI	CCCGGG	2 cut(s)	397, 572
TspRI	CASTG	3 cut(s)	31, 545, 949
Van91I	CCANNNNNTGG	2 cut(s)	895, 1270
VneI	GTGCAC	1 cut(s)	1577
VpaK11BI	GGWCC	2 cut(s)	314, 401
XapI	RAATTY	1 cut(s)	1636
XceI	RCATGY	2 cut(s)	211, 1117
XcmI	CCANNNNNNNNNTGG	1 cut(s)	705
XmaI	CCCGGG	2 cut(s)	397, 572
XmiI	GTMKAC	1 cut(s)	1107
XspI	CTAG	2 cut(s)	812, 858

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.