Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0006919

Physical Location & Seq

Genomic Coordinates Forward (+)

29945855 .. 29947618

Gene Structure 1764 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g29530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGCTTCATCTCCCTTACCACCAACTTCTACAATGGCCGCCCTGCACTCCACCACCACAACCACCCTCTTCCGCTCTCCTTTATTCCCAAACAAGTCCCAGACTCCACTGCAACGAAAACCCAAACAATGCCTTGCGGGCAGAGTGCGCTGCAAAGCAACAAAAGGTGACAGCGATAACCTAGACCAGGGCCTAGCAAGACTCGACAGGAGGAACATGCTGATAGGTTTAGGCGCCGGGGGTCTCTACGGTGCGGCAGGAAACCCATTTGCTTTTGCCGCACCAATATCCGCCCCAGACCTGACCAAGTGTGGCCCTGCCGACAAGCCAGACGGGTCCACCATCGATTGTTGCCCACCTACCACCACGACCATCATCGACTTCAAACTCCCCGACAAAGGCCCACTCCGCACTAGGCCCGCTGCTCAGAACGTTGCAAAAGACCCTGCATACTTGGCCAAATACAAAAAGGCCGTCGAGCTGATGCGGGCACTTCCAGACGACGACCCGCGCAGTCACGCCCAACAGGCCATGGTACACTGCTCCTACTGCGACGGTGGATACCCACAAGTCGGATATTCGGATTTGGAGATCCAAGTTCACTACTGTTGGCTCTTCTTCCCGTTCCATCGTTGGTACCTCTACTTCTACGAGAAAATCTTGGGCGAGCTTATTGGGGACCCAACCTTCGCCCTCCCCTTCTGGAACTGGGACGCGCCAGCTGGCATGTACATTCCTGAGATTTTCACCGACACGTCGTCATCCCTCTACGACCAGTACAGAAATGCAGCGCATCAGCCCCCGAAGCTCCTGGACTTGAATTACAGCGGGACCGACGATGAAGTCGACGACCCCACACGAATCAAAGAGAACTTAACAACGATGTACCAGCAGATGGTGTCGAAGGCCACCTCTCACAGACTCTTCTACGGAGAGCCCTACAGCGCAGGGGACGACCCAAGCCCTGGCGCCGGAAACATTGAGAGCATTCCCCATAACAATATTCACTTTTGGACTGGCGACCCAACCCAGACAAACGGGGAGGACATGGGGGCCTTTTACTCTTCGGGGAGGGATCCGATGTTTTACTCCCACCATTCCAACGTGGACCGCATGTGGTCTATATATAAAGATAAGTTGGGCGGTACGGACATAGAAAAGACCGACTGGCTGGACACGGAGTTCTTGTTCTACGACGAGAAAAAGAATCTCGTGCGCGTCAAGGTTCGGGACTCGCTCGACACGAAAAAACTGGGGTACGTGTACGACGAGAAAGTCCCGATCCCGTGGCTGAAGTATAAGCCGACGGCTCGTAAGTCGACGAATAAGAGAAAGGCCTCGGTTTCATCTTCTGATCTTACTACGACGTTCCCTGCTGTACTGTCGGATACAATCAGCGTCGAGGTGACGAGGCCGTCTGTGACGAAGCGGACAGCTGCCCAGAAGAAGGCGCAGGACGAGGTGCTGGTGATTACGGGGATTGAGTTTGCCGGGAATGAGACTGTGAAGTTCGACGTGTATGTGAACGATGACGCGGACTCTCTGGCCGGGAAAGACAAGTCGGAGTTTGCTGGGAGTTTTGTTCACGTGCCGCATAAGCATAAGAAAAATATTAAGACGAACCTGCGGCTGAGCATTACGAGCTTGTTGGAGGAGTTGGCTGCCGAGACGGACAGCAGTTTGGTGGTGACTTTGGTGCCGAAAGTTGGGAAGGGGCCAATCACCATCGGAGGGTTTAGCATTGAGCTCATTAATACTACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

588

Amino Acids

65.14

Weight (kDa)

6.41

Isoelectric Point (pI)

40.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	171 - 378	6e-36	Common central domain of tyrosinase
PPO1_DWL	PF12142	384 - 436	3.1e-24	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	457 - 583	1.1e-46	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g04680
malus_domestica	MD05G1319800.v1.1 MD05G1320100.v1.1 MD05G1320200.v1.1 MD05G1320500.v1.1 MD05G1320800.v1.1 MD10G1298300.v1.1
prunus_persica	Prupe.4G041500_v2.0.a1 Prupe.4G041700_v2.0.a1
pyrus_communis	pycom05g29530 pycom05g29560 pycom10g24890 pycom10g24910 pycom10g25140
rosa_chinensis	RchiOBHm_Chr5g0007621
rosa_laevigata	RLG00000031479
rosa_multiflora	Rmu_co8439317.1_g000001 Rmu_sc0011744.1_g000006 Rmu_sc0021005.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068730
rosa_rugosa	Rorug04G0429400
rosa_samantha	Rh5AG057500 Rh5BG054400 Rh5CG064400 Rh5DG053600
rosa_wichuraiana	Rw5G006180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	842, 1274, 1414
Acc36I	ACCTGC	1 cut(s)	1632
Acc65I	GGTACC	1 cut(s)	636
AccB1I	GGYRCC	4 cut(s)	233, 636, 968, 1696
AccB7I	CCANNNNNTGG	2 cut(s)	895, 965
AccBSI	CCGCTC	1 cut(s)	75
AccI	GTMKAC	2 cut(s)	846, 1319
AccII	CGCG	4 cut(s)	511, 716, 1218, 1535
AclI	AACGTT	1 cut(s)	432
AclWI	GGATC	4 cut(s)	586, 1070, 1083, 1276
AcoI	YGGCCR	3 cut(s)	36, 456, 1545
AcuI	CTGAAG	1 cut(s)	1313
AcvI	CACGTG	1 cut(s)	1588
AcyI	GRCGYC	2 cut(s)	234, 969
AfaI	GTAC	9 cut(s)	537, 638, 731, 779, 887, 1147, 1259, 1265, 1380
AfiI	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
AflIII	ACRYGT	3 cut(s)	753, 1260, 1515
AgsI	TTSAA	2 cut(s)	385, 820
AhdI	GACNNNNNGTC	1 cut(s)	757
AjiI	CACGTC	2 cut(s)	756, 1516
AjnI	CCWGG	3 cut(s)	186, 810, 964
AloI	GAACNNNNNNTCC	4 cut(s)	1166, 1172, 1198, 1204
AluBI	AGCT	7 cut(s)	481, 670, 722, 808, 1436, 1644, 1747
AluI	AGCT	7 cut(s)	481, 670, 722, 808, 1436, 1644, 1747
Alw21I	GWGCWC	1 cut(s)	1749
Alw26I	GTCTC	3 cut(s)	248, 1493, 1661
AlwI	GGATC	4 cut(s)	586, 1070, 1083, 1276
ApeKI	GCWGC	5 cut(s)	150, 422, 788, 1436, 1661
ArsI	GACNNNNNNTTYG	4 cut(s)	459, 491, 671, 703
AseI	ATTAAT	1 cut(s)	1752
Asp718I	GGTACC	1 cut(s)	636
AspLEI	GCGC	9 cut(s)	150, 236, 513, 718, 793, 947, 971, 1218, 1453
AsuC2I	CCSGG	3 cut(s)	238, 1492, 1549
AsuHPI	GGTGA	6 cut(s)	179, 739, 1417, 1480, 1699, 1714
AvaII	GGWCC	4 cut(s)	336, 679, 831, 1108
BaeGI	GKGCMC	1 cut(s)	493
BalI	TGGCCA	1 cut(s)	458
BamHI	GGATCC	1 cut(s)	1075
BanI	GGYRCC	4 cut(s)	233, 636, 968, 1696
BanII	GRGCYC	2 cut(s)	939, 1749
BarI	GAAGNNNNNNTAC	2 cut(s)	629, 661
BauI	CACGAG	1 cut(s)	1211
BbrPI	CACGTG	1 cut(s)	1588
Bbv12I	GWGCWC	1 cut(s)	1749
BbvI	GCAGC	5 cut(s)	137, 409, 800, 1423, 1648
BccI	CCATC	5 cut(s)	350, 380, 636, 889, 1733
BceAI	ACGGC	3 cut(s)	458, 1323, 1399
BciT130I	CCWGG	3 cut(s)	188, 812, 966
BciVI	GTATCC	2 cut(s)	554, 1381
BcnI	CCSGG	3 cut(s)	238, 1492, 1549
BcoDI	GTCTC	3 cut(s)	248, 1493, 1661
BfaI	CTAG	3 cut(s)	182, 194, 414
BfmI	CTRYAG	1 cut(s)	940
BfoI	RGCGCY	2 cut(s)	237, 972
BfuAI	ACCTGC	1 cut(s)	1632
BfuI	GTATCC	2 cut(s)	554, 1381
BglI	GCCNNNNNGGC	2 cut(s)	138, 527
BlpI	GCTNAGC	1 cut(s)	1631
Bme1390I	CCNGG	6 cut(s)	188, 238, 812, 966, 1492, 1549
Bme18I	GGWCC	4 cut(s)	336, 679, 831, 1108
BmeRI	GACNNNNNGTC	1 cut(s)	757
BmgBI	CACGTC	2 cut(s)	756, 1516
BmrFI	CCNGG	6 cut(s)	188, 238, 812, 966, 1492, 1549
BmrI	ACTGGG	2 cut(s)	718, 1262
BmsI	GCATC	2 cut(s)	474, 802
BmuI	ACTGGG	2 cut(s)	718, 1262
Bpu1102I	GCTNAGC	1 cut(s)	1631
BpuMI	CCSGG	3 cut(s)	238, 1492, 1549
Bsa29I	ATCGAT	1 cut(s)	345
BsaAI	YACGTR	2 cut(s)	1261, 1588
BsaHI	GRCGYC	2 cut(s)	234, 969
BsaI	GGTCTC	1 cut(s)	248
BsaJI	CCNNGG	6 cut(s)	187, 237, 531, 964, 1286, 1338
BsaXI	ACNNNNNCTCC	2 cut(s)	1172, 1202
Bsc4I	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
Bse1I	ACTGG	5 cut(s)	713, 775, 1021, 1172, 1257
BseBI	CCWGG	3 cut(s)	188, 812, 966
BseCI	ATCGAT	1 cut(s)	345
BseDI	CCNNGG	6 cut(s)	187, 237, 531, 964, 1286, 1338
BseGI	GGATG	1 cut(s)	761
BseLI	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
BseMII	CTCAG	3 cut(s)	440, 729, 1622
BseNI	ACTGG	5 cut(s)	713, 775, 1021, 1172, 1257
BseRI	GAGGAG	1 cut(s)	1667
BseSI	GKGCMC	1 cut(s)	493
BseXI	GCAGC	5 cut(s)	137, 409, 800, 1423, 1648
BseYI	CCCAGC	1 cut(s)	1571
BsgI	GTGCAG	1 cut(s)	29
Bsh1236I	CGCG	4 cut(s)	511, 716, 1218, 1535
BshNI	GGYRCC	4 cut(s)	233, 636, 968, 1696
BshVI	ATCGAT	1 cut(s)	345
BsiHKAI	GWGCWC	1 cut(s)	1749
BsiSI	CCGG	4 cut(s)	237, 972, 1491, 1548
BslFI	GGGAC	7 cut(s)	82, 692, 725, 844, 965, 1244, 1262
BslI	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
BsmAI	GTCTC	3 cut(s)	248, 1493, 1661
BsmBI	CGTCTC	1 cut(s)	1661
BsmFI	GGGAC	7 cut(s)	82, 692, 725, 844, 965, 1244, 1262
BsmI	GAATGC	1 cut(s)	987
Bso31I	GGTCTC	1 cut(s)	248
Bsp1286I	GDGCHC	3 cut(s)	493, 939, 1749
Bsp1407I	TGTACA	1 cut(s)	729
Bsp143I	GATC	4 cut(s)	591, 1075, 1281, 1354
Bsp1720I	GCTNAGC	1 cut(s)	1631
Bsp19I	CCATGG	1 cut(s)	531
BspCNI	CTCAG	3 cut(s)	439, 730, 1623
BspDI	ATCGAT	1 cut(s)	345
BspFNI	CGCG	4 cut(s)	511, 716, 1218, 1535
BspMI	ACCTGC	1 cut(s)	1632
BspPI	GGATC	4 cut(s)	586, 1070, 1083, 1276
BspQI	GCTCTTC	1 cut(s)	620
BspT107I	GGYRCC	4 cut(s)	233, 636, 968, 1696
BspTNI	GGTCTC	1 cut(s)	248
BsrBI	CCGCTC	1 cut(s)	75
BsrGI	TGTACA	1 cut(s)	729
BsrI	ACTGG	5 cut(s)	713, 775, 1021, 1172, 1257
BssECI	CCNNGG	6 cut(s)	187, 237, 531, 964, 1286, 1338
BssMI	GATC	4 cut(s)	591, 1075, 1281, 1354
BssNI	GRCGYC	2 cut(s)	234, 969
BssSI	CACGAG	1 cut(s)	1211
BssT1I	CCWWGG	1 cut(s)	531
Bst2BI	CACGAG	1 cut(s)	1211
Bst2UI	CCWGG	3 cut(s)	188, 812, 966
Bst4CI	ACNGT	5 cut(s)	251, 557, 608, 1383, 1504
Bst6I	CTCTTC	4 cut(s)	74, 620, 929, 1069
BstACI	GRCGYC	2 cut(s)	234, 969
BstAUI	TGTACA	1 cut(s)	729
BstBAI	YACGTR	2 cut(s)	1261, 1588
BstC8I	GCNNGC	6 cut(s)	139, 420, 489, 668, 720, 724
BstDEI	CTNAG	3 cut(s)	426, 738, 1631
BstDSI	CCRYGG	2 cut(s)	531, 1286
BstENI	CCTNNNNNAGG	1 cut(s)	185
BstF5I	GGATG	1 cut(s)	761
BstFNI	CGCG	4 cut(s)	511, 716, 1218, 1535
BstH2I	RGCGCY	2 cut(s)	237, 972
BstHHI	GCGC	9 cut(s)	150, 236, 513, 718, 793, 947, 971, 1218, 1453
BstKTI	GATC	4 cut(s)	594, 1078, 1284, 1357
BstMAI	GTCTC	3 cut(s)	248, 1493, 1661
BstMBI	GATC	4 cut(s)	591, 1075, 1281, 1354
BstNI	CCWGG	3 cut(s)	188, 812, 966
BstNSI	RCATGY	3 cut(s)	220, 730, 1117
BstSCI	CCNGG	6 cut(s)	186, 236, 810, 964, 1490, 1547
BstSFI	CTRYAG	1 cut(s)	940
BstSLI	GKGCMC	1 cut(s)	493
BstUI	CGCG	4 cut(s)	511, 716, 1218, 1535
BstV1I	GCAGC	5 cut(s)	137, 409, 800, 1423, 1648
BstX2I	RGATCY	2 cut(s)	591, 1075
BstYI	RGATCY	2 cut(s)	591, 1075
Bsu15I	ATCGAT	1 cut(s)	345
BsuI	GTATCC	2 cut(s)	554, 1381
BsuTUI	ATCGAT	1 cut(s)	345
BtgI	CCRYGG	2 cut(s)	531, 1286
BtrI	CACGTC	2 cut(s)	756, 1516
BtsCI	GGATG	1 cut(s)	761
BtsI	GCAGTG	2 cut(s)	107, 538
BtsIMutI	CAGTG	2 cut(s)	107, 538
BveI	ACCTGC	1 cut(s)	1632
Cac8I	GCNNGC	6 cut(s)	139, 420, 489, 668, 720, 724
CfoI	GCGC	9 cut(s)	150, 236, 513, 718, 793, 947, 971, 1218, 1453
ClaI	ATCGAT	1 cut(s)	345
CseI	GACGC	4 cut(s)	722, 1207, 1387, 1541
Csp6I	GTAC	9 cut(s)	536, 637, 730, 778, 886, 1146, 1258, 1264, 1379
CviAII	CATG	5 cut(s)	217, 532, 727, 1048, 1114
CviQI	GTAC	9 cut(s)	536, 637, 730, 778, 886, 1146, 1258, 1264, 1379
DdeI	CTNAG	3 cut(s)	426, 738, 1631
DinI	GGCGCC	2 cut(s)	235, 970
DpnI	GATC	4 cut(s)	593, 1077, 1283, 1356
DpnII	GATC	4 cut(s)	591, 1075, 1281, 1354
DrdI	GACNNNNNNGTC	3 cut(s)	842, 1274, 1414
DriI	GACNNNNNGTC	1 cut(s)	757
DseDI	GACNNNNNNGTC	3 cut(s)	842, 1274, 1414
EaeI	YGGCCR	3 cut(s)	36, 456, 1545
Eam1104I	CTCTTC	4 cut(s)	74, 620, 929, 1069
Eam1105I	GACNNNNNGTC	1 cut(s)	757
EarI	CTCTTC	4 cut(s)	74, 620, 929, 1069
EciI	GGCGGA	1 cut(s)	280
Ecl136II	GAGCTC	1 cut(s)	1747
Eco130I	CCWWGG	1 cut(s)	531
Eco147I	AGGCCT	1 cut(s)	1337
Eco24I	GRGCYC	2 cut(s)	939, 1749
Eco31I	GGTCTC	1 cut(s)	248
Eco47I	GGWCC	4 cut(s)	336, 679, 831, 1108
Eco53kI	GAGCTC	1 cut(s)	1747
Eco57I	CTGAAG	1 cut(s)	1313
Eco72I	CACGTG	1 cut(s)	1588
EcoICRI	GAGCTC	1 cut(s)	1747
EcoNI	CCTNNNNNAGG	1 cut(s)	185
EcoO109I	RGGNCCY	3 cut(s)	190, 679, 1053
EcoRII	CCWGG	3 cut(s)	186, 810, 964
EcoT14I	CCWWGG	1 cut(s)	531
EcoT38I	GRGCYC	2 cut(s)	939, 1749
EgeI	GGCGCC	2 cut(s)	235, 970
EheI	GGCGCC	2 cut(s)	235, 970
ErhI	CCWWGG	1 cut(s)	531
Esp3I	CGTCTC	1 cut(s)	1661
FaeI	CATG	5 cut(s)	220, 535, 730, 1051, 1117
FaqI	GGGAC	7 cut(s)	82, 692, 725, 844, 965, 1244, 1262
FatI	CATG	5 cut(s)	216, 531, 726, 1047, 1113
FauI	CCCGC	5 cut(s)	130, 427, 480, 516, 821
FblI	GTMKAC	2 cut(s)	846, 1319
FokI	GGATG	1 cut(s)	748
FriOI	GRGCYC	2 cut(s)	939, 1749
FspBI	CTAG	3 cut(s)	182, 194, 414
GlaI	GCGC	9 cut(s)	149, 235, 512, 717, 792, 946, 970, 1217, 1452
GsaI	CCCAGC	1 cut(s)	1575
HaeII	RGCGCY	2 cut(s)	237, 972
HapII	CCGG	4 cut(s)	237, 972, 1491, 1548
HgaI	GACGC	4 cut(s)	722, 1207, 1387, 1541
HhaI	GCGC	9 cut(s)	150, 236, 513, 718, 793, 947, 971, 1218, 1453
Hin1I	GRCGYC	2 cut(s)	234, 969
Hin1II	CATG	5 cut(s)	220, 535, 730, 1051, 1117
Hin6I	GCGC	9 cut(s)	148, 234, 511, 716, 791, 945, 969, 1216, 1451
HinP1I	GCGC	9 cut(s)	148, 234, 511, 716, 791, 945, 969, 1216, 1451
HincII	GTYRAC	2 cut(s)	847, 1320
HindII	GTYRAC	2 cut(s)	847, 1320
HinfI	GANTC	7 cut(s)	103, 201, 861, 921, 1207, 1232, 1538
HpaII	CCGG	4 cut(s)	237, 972, 1491, 1548
HphI	GGTGA	6 cut(s)	179, 739, 1417, 1480, 1699, 1714
Hpy166II	GTNNAC	9 cut(s)	339, 538, 601, 847, 1108, 1264, 1320, 1525, 1585
Hpy188I	TCNGA	8 cut(s)	429, 575, 583, 1080, 1354, 1387, 1564, 1730
Hpy188III	TCNNGA	5 cut(s)	497, 703, 737, 1229, 1279
Hpy8I	GTNNAC	9 cut(s)	339, 538, 601, 847, 1108, 1264, 1320, 1525, 1585
HpyAV	CCTTC	5 cut(s)	697, 709, 898, 1441, 1705
HpyCH4III	ACNGT	5 cut(s)	251, 557, 608, 1383, 1504
HpyCH4IV	ACGT	7 cut(s)	432, 755, 1104, 1260, 1367, 1515, 1587
HpyCH4V	TGCA	6 cut(s)	46, 112, 153, 437, 449, 788
HpyF3I	CTNAG	3 cut(s)	426, 738, 1631
HpySE526I	ACGT	7 cut(s)	432, 755, 1104, 1260, 1367, 1515, 1587
Hsp92I	GRCGYC	2 cut(s)	234, 969
Hsp92II	CATG	5 cut(s)	220, 535, 730, 1051, 1117
HspAI	GCGC	9 cut(s)	148, 234, 511, 716, 791, 945, 969, 1216, 1451
KasI	GGCGCC	2 cut(s)	233, 968
KflI	GGGWCCC	1 cut(s)	679
KpnI	GGTACC	1 cut(s)	640
Kzo9I	GATC	4 cut(s)	591, 1075, 1281, 1354
LguI	GCTCTTC	1 cut(s)	620
LmnI	GCTCC	2 cut(s)	548, 813
Lsp1109I	GCAGC	5 cut(s)	137, 409, 800, 1423, 1648
LweI	GCATC	2 cut(s)	474, 802
MaeI	CTAG	3 cut(s)	182, 194, 414
MaeII	ACGT	7 cut(s)	432, 755, 1104, 1260, 1367, 1515, 1587
MaeIII	GTNAC	5 cut(s)	167, 515, 1405, 1420, 1687
MalI	GATC	4 cut(s)	593, 1077, 1283, 1356
MbiI	CCGCTC	1 cut(s)	75
MboI	GATC	4 cut(s)	591, 1075, 1281, 1354
MboII	GAAGA	7 cut(s)	61, 607, 610, 916, 1056, 1341, 1456
MflI	RGATCY	2 cut(s)	591, 1075
MhlI	GDGCHC	3 cut(s)	493, 939, 1749
MlsI	TGGCCA	1 cut(s)	458
MluCI	AATT	1 cut(s)	820
MluNI	TGGCCA	1 cut(s)	458
Mly113I	GGCGCC	2 cut(s)	234, 969
MlyI	GAGTC	5 cut(s)	97, 195, 915, 1226, 1532
MmeI	TCCRAC	5 cut(s)	553, 1125, 1365, 1542, 1629
Mox20I	TGGCCA	1 cut(s)	458
MscI	TGGCCA	1 cut(s)	458
MseI	TTAA	3 cut(s)	875, 1614, 1752
Msp20I	TGGCCA	1 cut(s)	458
MspA1I	CMGCKG	4 cut(s)	422, 722, 828, 1436
MspI	CCGG	4 cut(s)	237, 972, 1491, 1548
MspR9I	CCNGG	6 cut(s)	188, 238, 812, 966, 1492, 1549
Mva1269I	GAATGC	1 cut(s)	987
MvaI	CCWGG	3 cut(s)	188, 812, 966
MvnI	CGCG	4 cut(s)	511, 716, 1218, 1535
NarI	GGCGCC	2 cut(s)	234, 969
NciI	CCSGG	3 cut(s)	238, 1492, 1549
NcoI	CCATGG	1 cut(s)	531
NdeII	GATC	4 cut(s)	591, 1075, 1281, 1354
NlaIII	CATG	5 cut(s)	220, 535, 730, 1051, 1117
NmeAIII	GCCGAG	1 cut(s)	1690
NmuCI	GTSAC	5 cut(s)	167, 515, 1405, 1420, 1687
NspI	RCATGY	3 cut(s)	220, 730, 1117
PceI	AGGCCT	1 cut(s)	1337
PciSI	GCTCTTC	1 cut(s)	620
PcsI	WCGNNNNNNNCGW	3 cut(s)	843, 1241, 1266
PctI	GAATGC	1 cut(s)	987
PfeI	GAWTC	2 cut(s)	861, 1207
PflMI	CCANNNNNTGG	2 cut(s)	895, 965
PfoI	TCCNGGA	1 cut(s)	810
PleI	GAGTC	5 cut(s)	97, 195, 915, 1226, 1532
PluTI	GGCGCC	2 cut(s)	237, 972
PmaCI	CACGTG	1 cut(s)	1588
PmlI	CACGTG	1 cut(s)	1588
PpsI	GAGTC	5 cut(s)	97, 195, 915, 1226, 1532
Ppu21I	YACGTR	2 cut(s)	1261, 1588
PpuMI	RGGWCCY	1 cut(s)	679
PshBI	ATTAAT	1 cut(s)	1752
Psp124BI	GAGCTC	1 cut(s)	1749
Psp1406I	AACGTT	1 cut(s)	432
Psp5II	RGGWCCY	1 cut(s)	679
Psp6I	CCWGG	3 cut(s)	186, 810, 964
PspCI	CACGTG	1 cut(s)	1588
PspFI	CCCAGC	1 cut(s)	1571
PspGI	CCWGG	3 cut(s)	186, 810, 964
PspPPI	RGGWCCY	1 cut(s)	679
PsuI	RGATCY	2 cut(s)	591, 1075
PvuII	CAGCTG	2 cut(s)	722, 1436
RsaI	GTAC	9 cut(s)	537, 638, 731, 779, 887, 1147, 1259, 1265, 1380
RsaNI	GTAC	9 cut(s)	536, 637, 730, 778, 886, 1146, 1258, 1264, 1379
SacI	GAGCTC	1 cut(s)	1749
SalI	GTCGAC	2 cut(s)	845, 1318
SapI	GCTCTTC	1 cut(s)	620
SaqAI	TTAA	3 cut(s)	875, 1614, 1752
Sau3AI	GATC	4 cut(s)	591, 1075, 1281, 1354
SchI	GAGTC	5 cut(s)	97, 195, 915, 1226, 1532
ScrFI	CCNGG	6 cut(s)	188, 238, 812, 966, 1492, 1549
SduI	GDGCHC	3 cut(s)	493, 939, 1749
SfaNI	GCATC	2 cut(s)	474, 802
SfcI	CTRYAG	1 cut(s)	940
SfoI	GGCGCC	2 cut(s)	235, 970
SinI	GGWCC	4 cut(s)	336, 679, 831, 1108
Sse9I	AATT	1 cut(s)	820
SseBI	AGGCCT	1 cut(s)	1337
SspDI	GGCGCC	2 cut(s)	233, 968
SspI	AATATT	2 cut(s)	1003, 1612
SspMI	CTAG	3 cut(s)	182, 194, 414
SstI	GAGCTC	1 cut(s)	1749
StuI	AGGCCT	1 cut(s)	1337
StyD4I	CCNGG	6 cut(s)	186, 236, 810, 964, 1490, 1547
StyI	CCWWGG	1 cut(s)	531
TaaI	ACNGT	5 cut(s)	251, 557, 608, 1383, 1504
TaiI	ACGT	7 cut(s)	435, 758, 1107, 1263, 1370, 1518, 1590
TaqII	GACCGA	2 cut(s)	848, 1178
TasI	AATT	1 cut(s)	820
TatI	WGTACW	3 cut(s)	729, 777, 1378
TauI	GCSGC	5 cut(s)	41, 257, 281, 1594, 1630
TfiI	GAWTC	2 cut(s)	861, 1207
Tru1I	TTAA	3 cut(s)	875, 1614, 1752
Tru9I	TTAA	3 cut(s)	875, 1614, 1752
TscAI	CASTG	2 cut(s)	114, 545
TseFI	GTSAC	5 cut(s)	167, 515, 1405, 1420, 1687
TseI	GCWGC	5 cut(s)	150, 422, 788, 1436, 1661
Tsp45I	GTSAC	5 cut(s)	167, 515, 1405, 1420, 1687
TspDTI	ATGAA	2 cut(s)	855, 1335
TspGWI	ACGGA	4 cut(s)	945, 1163, 1193, 1685
TspRI	CASTG	2 cut(s)	114, 545
Van91I	CCANNNNNTGG	2 cut(s)	895, 965
VpaK11BI	GGWCC	4 cut(s)	336, 679, 831, 1108
VspI	ATTAAT	1 cut(s)	1752
XagI	CCTNNNNNAGG	1 cut(s)	185
XceI	RCATGY	3 cut(s)	220, 730, 1117
XmiI	GTMKAC	2 cut(s)	846, 1319
XspI	CTAG	3 cut(s)	182, 194, 414

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.