Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0006919

Physical Location & Seq

Genomic Coordinates Forward (+)

29981707 .. 29983467

Gene Structure 1761 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g29560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGGCTTCATCTCCCTTACCACCAACTTCTACAATGGCCGCCCTGCACTCTACCACAACTACCCTCTTCCGCTCTCCTTTATTCCCAAACAAATCCCAGACTCCACTGCAAGGAAAACCCAAACAATGCCTTGCGGGCAGAGTGCGCTGCAAAGCAACAAAAGGTGACAATGATAACCCAGACCAGGGCCTAGCAAGACTCGACAGGAGGAACATGCTGATAGGTTTAGGCGCCGGGGGTCTCTACGGTGCGGCAGGAAACCCATTTGCTTTTGCCGCACCAGTATCCGCTCCAGACCTGACCACTTGTGGCCCTGCCGACAAGCCAGACGGGTCCACCATCGATTGTTGCCCACCTAGCACCACGACCATCATCGACTTCGAACTCCCCGACTCAGGCCCACTCCGCACTAGGCCCGCTGCCCAGGACGTTGCAAACGACCCTGCATACTTGGCCAAATACAAAAAGGCTGTCGAGCTGATGCGGGCACTTCCAGACGATGACCCGCGCAGTCACGCCCAACAGGCCATGGTACACTGCTCCTACTGCGACGGTGCATACCCACAAGTCGGATATTCGGATTTGGAAATCCAAGTTCACTACTGTTGGCTCTTCTTCCCGTTCCATCGTTGGTACCTCTACTTCTACGAGAAAATCTTGGGCGAGCTCATTGGGGACCCAACCTTCGCCCTCCCCTTCTGGAACTGGGACGCGCCAGCTGGCATGTACATTCCTGAGATTTTCACCGATACGTCGTCATCCCTCTACGACCAGTACAGAAATGCAGCGCATCAGCCCCCGAAGCTCCTGGACTTGAATTACAGCGGGACCGACGATGACGTCGACGACGCGACACGAATCACAGAGAACTTAACAACGATGTATCAGCAGATGGTGTCGAAGGCCACTTCTCACAGACTCTTCTACGGAGAGCCCTACAGCGCAGGGGACGACCCAAGCCCTGGCGCCGGAAACATTGAGAGCATTCCCCATAACAATATTCACTTTTGGACTGGCGACCCAACCCAGACAAACGGGGAGGACATGGGGGCCTTTTACTCTTCGGGGAGGGATCCGATGTTTTACTCCCACCATTCCAACGTGGACCGCATGTGGTCTATATATAAAGATAAGTTGGGCGGTACGTACATAGAAAAGACCGACTGGCTGGACACGGAGTTCTTGTTCTACGACGAGAAAAAGAATCTCGTGCGCGTCAAGGTTCGGGACTCGCTCGACACGAAAAAACTCGGGTACGTGTACGACGAGAATGTCTCGATCCCGTGGTTGGAGTATAAGCCGACGGCTCGTAAGTCGACGAATAAGAGAAAGGCCACGGTTTCATCTTCTGATCTTACTGCAACGTTCCCTGCTACACTGTCGGATACAATCAGCGTCGAGGTGACGAGGCCGTCTGCAACGAAGCGGACAGCTGCCCAAAAGAAGGCGCAGGACGAGGTGCTGGTGATTACGGGGATTGAGTTTGCCGGGAATGAGACTGTGAAGTTCGACGTGTATGTGAACGATGACGCGGACTCGCTGGCCGGGAAAGACAAGTCGGAGTTTGCTGGGAGTTTTGTTCACGTGCCGCATAAGCATAAGAAAAATATTAAGACGAACCTGCGGCTGAGCATTACGAGCTTGTTGGAGGAGTTGGATGCGGAGACAGACAGCACTTTGGTGGTGACTTTGGTGCCGAAAGTTGGGAAGGGGCCCATCACCATCGGAGGGTTTAGCATTGAGCTCATTAATACTACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

587

Amino Acids

64.83

Weight (kDa)

5.44

Isoelectric Point (pI)

42.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	170 - 377	1.6e-35	Common central domain of tyrosinase
PPO1_DWL	PF12142	386 - 435	2.3e-23	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	456 - 582	2.2e-47	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g04680
malus_domestica	MD05G1319800.v1.1 MD05G1320100.v1.1 MD05G1320200.v1.1 MD05G1320500.v1.1 MD05G1320800.v1.1 MD10G1298300.v1.1
prunus_persica	Prupe.4G041500_v2.0.a1 Prupe.4G041700_v2.0.a1
pyrus_communis	pycom05g29530 pycom05g29560 pycom10g24890 pycom10g24910 pycom10g25140
rosa_chinensis	RchiOBHm_Chr5g0007621
rosa_laevigata	RLG00000031479
rosa_multiflora	Rmu_co8439317.1_g000001 Rmu_sc0011744.1_g000006 Rmu_sc0021005.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068730
rosa_rugosa	Rorug04G0429400
rosa_samantha	Rh5AG057500 Rh5BG054400 Rh5CG064400 Rh5DG053600
rosa_wichuraiana	Rw5G006180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	839, 1271, 1411
AatII	GACGTC	1 cut(s)	843
Acc36I	ACCTGC	1 cut(s)	1629
Acc65I	GGTACC	1 cut(s)	633
AccB1I	GGYRCC	4 cut(s)	230, 633, 965, 1693
AccB7I	CCANNNNNTGG	1 cut(s)	962
AccBSI	CCGCTC	2 cut(s)	72, 290
AccI	GTMKAC	2 cut(s)	843, 1316
AccII	CGCG	5 cut(s)	508, 713, 851, 1215, 1532
AclI	AACGTT	1 cut(s)	1364
AclWI	GGATC	3 cut(s)	1067, 1080, 1273
AcoI	YGGCCR	3 cut(s)	36, 453, 1542
AcvI	CACGTG	1 cut(s)	1585
AcyI	GRCGYC	3 cut(s)	231, 840, 966
AfaI	GTAC	8 cut(s)	534, 635, 728, 776, 1144, 1148, 1256, 1262
AflIII	ACRYGT	2 cut(s)	1257, 1512
AgsI	TTSAA	1 cut(s)	817
AjiI	CACGTC	1 cut(s)	1513
AjnI	CCWGG	4 cut(s)	183, 423, 807, 961
AleI	CACNNNNGTG	1 cut(s)	1679
AloI	GAACNNNNNNTCC	4 cut(s)	1163, 1169, 1195, 1201
AluBI	AGCT	7 cut(s)	478, 667, 719, 805, 1433, 1641, 1744
AluI	AGCT	7 cut(s)	478, 667, 719, 805, 1433, 1641, 1744
Alw21I	GWGCWC	2 cut(s)	669, 1746
Alw26I	GTCTC	4 cut(s)	245, 1279, 1490, 1658
AlwI	GGATC	3 cut(s)	1067, 1080, 1273
Ama87I	CYCGRG	1 cut(s)	1250
ApaI	GGGCCC	1 cut(s)	1716
ApeKI	GCWGC	4 cut(s)	147, 419, 785, 1433
ArsI	GACNNNNNNTTYG	4 cut(s)	456, 488, 668, 700
AseI	ATTAAT	1 cut(s)	1749
Asp718I	GGTACC	1 cut(s)	633
AspLEI	GCGC	9 cut(s)	147, 233, 510, 715, 790, 944, 968, 1215, 1450
AsuC2I	CCSGG	3 cut(s)	235, 1489, 1546
AsuHPI	GGTGA	6 cut(s)	176, 736, 1414, 1477, 1696, 1711
AsuII	TTCGAA	1 cut(s)	381
AvaI	CYCGRG	1 cut(s)	1250
AvaII	GGWCC	4 cut(s)	333, 676, 828, 1105
BaeGI	GKGCMC	2 cut(s)	490, 1716
BalI	TGGCCA	1 cut(s)	455
BamHI	GGATCC	1 cut(s)	1072
BanI	GGYRCC	4 cut(s)	230, 633, 965, 1693
BanII	GRGCYC	4 cut(s)	669, 936, 1716, 1746
BarI	GAAGNNNNNNTAC	2 cut(s)	626, 658
BauI	CACGAG	1 cut(s)	1208
BbrPI	CACGTG	1 cut(s)	1585
Bbv12I	GWGCWC	2 cut(s)	669, 1746
BbvI	GCAGC	4 cut(s)	134, 406, 797, 1420
BccI	CCATC	6 cut(s)	347, 377, 633, 886, 1724, 1730
BceAI	ACGGC	2 cut(s)	1320, 1396
BciT130I	CCWGG	4 cut(s)	185, 425, 809, 963
BciVI	GTATCC	2 cut(s)	295, 1378
BcnI	CCSGG	3 cut(s)	235, 1489, 1546
BcoDI	GTCTC	4 cut(s)	245, 1279, 1490, 1658
BfaI	CTAG	3 cut(s)	191, 357, 411
BfmI	CTRYAG	1 cut(s)	937
BfoI	RGCGCY	2 cut(s)	234, 969
BfuAI	ACCTGC	1 cut(s)	1629
BfuI	GTATCC	2 cut(s)	295, 1378
BglI	GCCNNNNNGGC	2 cut(s)	135, 524
BisI	GCNGC	9 cut(s)	39, 148, 252, 276, 420, 786, 1434, 1589, 1625
BlpI	GCTNAGC	1 cut(s)	1628
BlsI	GCNGC	9 cut(s)	40, 149, 253, 277, 421, 787, 1435, 1590, 1626
Bme1390I	CCNGG	7 cut(s)	185, 235, 425, 809, 963, 1489, 1546
Bme18I	GGWCC	4 cut(s)	333, 676, 828, 1105
BmeT110I	CYCGRG	1 cut(s)	1250
BmgBI	CACGTC	1 cut(s)	1513
BmrFI	CCNGG	7 cut(s)	185, 235, 425, 809, 963, 1489, 1546
BmrI	ACTGGG	1 cut(s)	715
BmsI	GCATC	3 cut(s)	471, 799, 1648
BmuI	ACTGGG	1 cut(s)	715
BplI	GAGNNNNNCTC	2 cut(s)	1259, 1291
BpmI	CTGGAG	1 cut(s)	276
Bpu1102I	GCTNAGC	1 cut(s)	1628
Bpu14I	TTCGAA	1 cut(s)	381
BpuMI	CCSGG	3 cut(s)	235, 1489, 1546
Bsa29I	ATCGAT	1 cut(s)	342
BsaAI	YACGTR	3 cut(s)	1146, 1258, 1585
BsaHI	GRCGYC	3 cut(s)	231, 840, 966
BsaI	GGTCTC	1 cut(s)	245
BsaJI	CCNNGG	7 cut(s)	184, 234, 423, 528, 961, 1283, 1335
BsaXI	ACNNNNNCTCC	2 cut(s)	1169, 1199
Bse1I	ACTGG	5 cut(s)	281, 710, 772, 1018, 1169
BseBI	CCWGG	4 cut(s)	185, 425, 809, 963
BseCI	ATCGAT	1 cut(s)	342
BseDI	CCNNGG	7 cut(s)	184, 234, 423, 528, 961, 1283, 1335
BseGI	GGATG	2 cut(s)	758, 1663
BseMII	CTCAG	3 cut(s)	408, 726, 1619
BseNI	ACTGG	5 cut(s)	281, 710, 772, 1018, 1169
BseRI	GAGGAG	1 cut(s)	1664
BseSI	GKGCMC	2 cut(s)	490, 1716
BseXI	GCAGC	4 cut(s)	134, 406, 797, 1420
BseYI	CCCAGC	1 cut(s)	1568
BsgI	GTGCAG	1 cut(s)	29
Bsh1236I	CGCG	5 cut(s)	508, 713, 851, 1215, 1532
BshNI	GGYRCC	4 cut(s)	230, 633, 965, 1693
BshVI	ATCGAT	1 cut(s)	342
BsiHKAI	GWGCWC	2 cut(s)	669, 1746
BsiHKCI	CYCGRG	1 cut(s)	1250
BsiSI	CCGG	4 cut(s)	234, 969, 1488, 1545
BslFI	GGGAC	5 cut(s)	689, 722, 841, 962, 1241
BsmAI	GTCTC	4 cut(s)	245, 1279, 1490, 1658
BsmFI	GGGAC	5 cut(s)	689, 722, 841, 962, 1241
BsmI	GAATGC	1 cut(s)	984
Bso31I	GGTCTC	1 cut(s)	245
BsoBI	CYCGRG	1 cut(s)	1250
Bsp119I	TTCGAA	1 cut(s)	381
Bsp120I	GGGCCC	1 cut(s)	1712
Bsp1286I	GDGCHC	5 cut(s)	490, 669, 936, 1716, 1746
Bsp1407I	TGTACA	1 cut(s)	726
Bsp143I	GATC	3 cut(s)	1072, 1278, 1351
Bsp1720I	GCTNAGC	1 cut(s)	1628
Bsp19I	CCATGG	1 cut(s)	528
BspCNI	CTCAG	3 cut(s)	407, 727, 1620
BspDI	ATCGAT	1 cut(s)	342
BspFNI	CGCG	5 cut(s)	508, 713, 851, 1215, 1532
BspMI	ACCTGC	1 cut(s)	1629
BspPI	GGATC	3 cut(s)	1067, 1080, 1273
BspQI	GCTCTTC	1 cut(s)	617
BspT104I	TTCGAA	1 cut(s)	381
BspT107I	GGYRCC	4 cut(s)	230, 633, 965, 1693
BspTNI	GGTCTC	1 cut(s)	245
BsrBI	CCGCTC	2 cut(s)	72, 290
BsrGI	TGTACA	1 cut(s)	726
BsrI	ACTGG	5 cut(s)	281, 710, 772, 1018, 1169
BssECI	CCNNGG	7 cut(s)	184, 234, 423, 528, 961, 1283, 1335
BssMI	GATC	3 cut(s)	1072, 1278, 1351
BssNI	GRCGYC	3 cut(s)	231, 840, 966
BssSI	CACGAG	1 cut(s)	1208
BssT1I	CCWWGG	1 cut(s)	528
Bst2BI	CACGAG	1 cut(s)	1208
Bst2UI	CCWGG	4 cut(s)	185, 425, 809, 963
Bst4CI	ACNGT	6 cut(s)	248, 554, 605, 1339, 1380, 1501
Bst6I	CTCTTC	4 cut(s)	71, 617, 926, 1066
BstACI	GRCGYC	3 cut(s)	231, 840, 966
BstAUI	TGTACA	1 cut(s)	726
BstBAI	YACGTR	3 cut(s)	1146, 1258, 1585
BstBI	TTCGAA	1 cut(s)	381
BstC8I	GCNNGC	7 cut(s)	136, 417, 486, 665, 717, 721, 1542
BstDEI	CTNAG	3 cut(s)	394, 735, 1628
BstDSI	CCRYGG	3 cut(s)	528, 1283, 1335
BstF5I	GGATG	2 cut(s)	758, 1663
BstFNI	CGCG	5 cut(s)	508, 713, 851, 1215, 1532
BstH2I	RGCGCY	2 cut(s)	234, 969
BstHHI	GCGC	9 cut(s)	147, 233, 510, 715, 790, 944, 968, 1215, 1450
BstKTI	GATC	3 cut(s)	1075, 1281, 1354
BstMAI	GTCTC	4 cut(s)	245, 1279, 1490, 1658
BstMBI	GATC	3 cut(s)	1072, 1278, 1351
BstNI	CCWGG	4 cut(s)	185, 425, 809, 963
BstNSI	RCATGY	3 cut(s)	217, 727, 1114
BstSCI	CCNGG	7 cut(s)	183, 233, 423, 807, 961, 1487, 1544
BstSFI	CTRYAG	1 cut(s)	937
BstSLI	GKGCMC	2 cut(s)	490, 1716
BstSNI	TACGTA	1 cut(s)	1146
BstUI	CGCG	5 cut(s)	508, 713, 851, 1215, 1532
BstV1I	GCAGC	4 cut(s)	134, 406, 797, 1420
BstX2I	RGATCY	1 cut(s)	1072
BstYI	RGATCY	1 cut(s)	1072
Bsu15I	ATCGAT	1 cut(s)	342
BsuI	GTATCC	2 cut(s)	295, 1378
BsuTUI	ATCGAT	1 cut(s)	342
BtgI	CCRYGG	3 cut(s)	528, 1283, 1335
BtrI	CACGTC	1 cut(s)	1513
BtsCI	GGATG	2 cut(s)	758, 1663
BtsI	GCAGTG	2 cut(s)	104, 535
BtsIMutI	CAGTG	3 cut(s)	104, 535, 1376
BveI	ACCTGC	1 cut(s)	1629
Cac8I	GCNNGC	7 cut(s)	136, 417, 486, 665, 717, 721, 1542
CfoI	GCGC	9 cut(s)	147, 233, 510, 715, 790, 944, 968, 1215, 1450
ClaI	ATCGAT	1 cut(s)	342
CseI	GACGC	5 cut(s)	719, 857, 1204, 1384, 1538
Csp6I	GTAC	8 cut(s)	533, 634, 727, 775, 1143, 1147, 1255, 1261
CviAII	CATG	5 cut(s)	214, 529, 724, 1045, 1111
CviQI	GTAC	8 cut(s)	533, 634, 727, 775, 1143, 1147, 1255, 1261
DdeI	CTNAG	3 cut(s)	394, 735, 1628
DinI	GGCGCC	2 cut(s)	232, 967
DpnI	GATC	3 cut(s)	1074, 1280, 1353
DpnII	GATC	3 cut(s)	1072, 1278, 1351
DrdI	GACNNNNNNGTC	3 cut(s)	839, 1271, 1411
DseDI	GACNNNNNNGTC	3 cut(s)	839, 1271, 1411
EaeI	YGGCCR	3 cut(s)	36, 453, 1542
Eam1104I	CTCTTC	4 cut(s)	71, 617, 926, 1066
EarI	CTCTTC	4 cut(s)	71, 617, 926, 1066
Ecl136II	GAGCTC	2 cut(s)	667, 1744
Eco105I	TACGTA	1 cut(s)	1146
Eco130I	CCWWGG	1 cut(s)	528
Eco24I	GRGCYC	4 cut(s)	669, 936, 1716, 1746
Eco31I	GGTCTC	1 cut(s)	245
Eco47I	GGWCC	4 cut(s)	333, 676, 828, 1105
Eco53kI	GAGCTC	2 cut(s)	667, 1744
Eco72I	CACGTG	1 cut(s)	1585
Eco88I	CYCGRG	1 cut(s)	1250
EcoICRI	GAGCTC	2 cut(s)	667, 1744
EcoO109I	RGGNCCY	4 cut(s)	187, 676, 1050, 1712
EcoRII	CCWGG	4 cut(s)	183, 423, 807, 961
EcoT14I	CCWWGG	1 cut(s)	528
EcoT38I	GRGCYC	4 cut(s)	669, 936, 1716, 1746
EgeI	GGCGCC	2 cut(s)	232, 967
EheI	GGCGCC	2 cut(s)	232, 967
ErhI	CCWWGG	1 cut(s)	528
FaeI	CATG	5 cut(s)	217, 532, 727, 1048, 1114
FaqI	GGGAC	5 cut(s)	689, 722, 841, 962, 1241
FatI	CATG	5 cut(s)	213, 528, 723, 1044, 1110
FauI	CCCGC	5 cut(s)	127, 424, 477, 513, 818
FblI	GTMKAC	2 cut(s)	843, 1316
Fnu4HI	GCNGC	9 cut(s)	39, 148, 252, 276, 420, 786, 1434, 1589, 1625
FokI	GGATG	2 cut(s)	745, 1670
FriOI	GRGCYC	4 cut(s)	669, 936, 1716, 1746
Fsp4HI	GCNGC	9 cut(s)	39, 148, 252, 276, 420, 786, 1434, 1589, 1625
FspBI	CTAG	3 cut(s)	191, 357, 411
GlaI	GCGC	9 cut(s)	146, 232, 509, 714, 789, 943, 967, 1214, 1449
GluI	GCNGC	9 cut(s)	39, 148, 252, 276, 420, 786, 1434, 1589, 1625
GsaI	CCCAGC	1 cut(s)	1572
GsuI	CTGGAG	1 cut(s)	276
HaeII	RGCGCY	2 cut(s)	234, 969
HapII	CCGG	4 cut(s)	234, 969, 1488, 1545
HgaI	GACGC	5 cut(s)	719, 857, 1204, 1384, 1538
HhaI	GCGC	9 cut(s)	147, 233, 510, 715, 790, 944, 968, 1215, 1450
Hin1I	GRCGYC	3 cut(s)	231, 840, 966
Hin1II	CATG	5 cut(s)	217, 532, 727, 1048, 1114
Hin6I	GCGC	9 cut(s)	145, 231, 508, 713, 788, 942, 966, 1213, 1448
HinP1I	GCGC	9 cut(s)	145, 231, 508, 713, 788, 942, 966, 1213, 1448
HincII	GTYRAC	2 cut(s)	844, 1317
HindII	GTYRAC	2 cut(s)	844, 1317
HinfI	GANTC	8 cut(s)	100, 198, 392, 858, 918, 1204, 1229, 1535
HpaII	CCGG	4 cut(s)	234, 969, 1488, 1545
HphI	GGTGA	6 cut(s)	176, 736, 1414, 1477, 1696, 1711
Hpy166II	GTNNAC	9 cut(s)	336, 535, 598, 844, 1105, 1261, 1317, 1522, 1582
Hpy188I	TCNGA	7 cut(s)	572, 580, 1077, 1351, 1384, 1561, 1727
Hpy188III	TCNNGA	6 cut(s)	293, 494, 700, 734, 1226, 1276
Hpy8I	GTNNAC	9 cut(s)	336, 535, 598, 844, 1105, 1261, 1317, 1522, 1582
HpyAV	CCTTC	5 cut(s)	694, 706, 895, 1438, 1702
HpyCH4III	ACNGT	6 cut(s)	248, 554, 605, 1339, 1380, 1501
HpyCH4IV	ACGT	9 cut(s)	429, 752, 840, 1101, 1145, 1257, 1364, 1512, 1584
HpyCH4V	TGCA	9 cut(s)	46, 109, 150, 434, 446, 557, 785, 1361, 1418
HpyF3I	CTNAG	3 cut(s)	394, 735, 1628
HpySE526I	ACGT	9 cut(s)	429, 752, 840, 1101, 1145, 1257, 1364, 1512, 1584
Hsp92I	GRCGYC	3 cut(s)	231, 840, 966
Hsp92II	CATG	5 cut(s)	217, 532, 727, 1048, 1114
HspAI	GCGC	9 cut(s)	145, 231, 508, 713, 788, 942, 966, 1213, 1448
KasI	GGCGCC	2 cut(s)	230, 965
KflI	GGGWCCC	1 cut(s)	676
KpnI	GGTACC	1 cut(s)	637
Kzo9I	GATC	3 cut(s)	1072, 1278, 1351
LguI	GCTCTTC	1 cut(s)	617
LmnI	GCTCC	3 cut(s)	295, 545, 810
Lsp1109I	GCAGC	4 cut(s)	134, 406, 797, 1420
LweI	GCATC	3 cut(s)	471, 799, 1648
MaeI	CTAG	3 cut(s)	191, 357, 411
MaeII	ACGT	9 cut(s)	429, 752, 840, 1101, 1145, 1257, 1364, 1512, 1584
MaeIII	GTNAC	4 cut(s)	164, 512, 1402, 1684
MalI	GATC	3 cut(s)	1074, 1280, 1353
MbiI	CCGCTC	2 cut(s)	72, 290
MboI	GATC	3 cut(s)	1072, 1278, 1351
MboII	GAAGA	6 cut(s)	58, 604, 607, 913, 1053, 1338
MflI	RGATCY	1 cut(s)	1072
MhlI	GDGCHC	5 cut(s)	490, 669, 936, 1716, 1746
MlsI	TGGCCA	1 cut(s)	455
MluCI	AATT	1 cut(s)	817
MluNI	TGGCCA	1 cut(s)	455
Mly113I	GGCGCC	2 cut(s)	231, 966
MlyI	GAGTC	6 cut(s)	94, 192, 386, 912, 1223, 1529
MmeI	TCCRAC	7 cut(s)	550, 1122, 1269, 1362, 1539, 1626, 1635
Mox20I	TGGCCA	1 cut(s)	455
MscI	TGGCCA	1 cut(s)	455
MseI	TTAA	3 cut(s)	872, 1611, 1749
MslI	CAYNNNNRTG	1 cut(s)	1679
Msp20I	TGGCCA	1 cut(s)	455
MspA1I	CMGCKG	4 cut(s)	419, 719, 825, 1433
MspI	CCGG	4 cut(s)	234, 969, 1488, 1545
MspR9I	CCNGG	7 cut(s)	185, 235, 425, 809, 963, 1489, 1546
Mva1269I	GAATGC	1 cut(s)	984
MvaI	CCWGG	4 cut(s)	185, 425, 809, 963
MvnI	CGCG	5 cut(s)	508, 713, 851, 1215, 1532
NarI	GGCGCC	2 cut(s)	231, 966
NciI	CCSGG	3 cut(s)	235, 1489, 1546
NcoI	CCATGG	1 cut(s)	528
NdeII	GATC	3 cut(s)	1072, 1278, 1351
NlaIII	CATG	5 cut(s)	217, 532, 727, 1048, 1114
NmuCI	GTSAC	4 cut(s)	164, 512, 1402, 1684
NspI	RCATGY	3 cut(s)	217, 727, 1114
NspV	TTCGAA	1 cut(s)	381
OliI	CACNNNNGTG	1 cut(s)	1679
PciSI	GCTCTTC	1 cut(s)	617
PcsI	WCGNNNNNNNCGW	5 cut(s)	387, 435, 840, 1238, 1263
PctI	GAATGC	1 cut(s)	984
PfeI	GAWTC	2 cut(s)	858, 1204
PflMI	CCANNNNNTGG	1 cut(s)	962
PfoI	TCCNGGA	1 cut(s)	807
PkrI	GCNGC	9 cut(s)	40, 149, 253, 277, 421, 787, 1435, 1590, 1626
PleI	GAGTC	6 cut(s)	94, 192, 386, 912, 1223, 1529
PluTI	GGCGCC	2 cut(s)	234, 969
PmaCI	CACGTG	1 cut(s)	1585
PmlI	CACGTG	1 cut(s)	1585
PpsI	GAGTC	6 cut(s)	94, 192, 386, 912, 1223, 1529
Ppu21I	YACGTR	3 cut(s)	1146, 1258, 1585
PpuMI	RGGWCCY	1 cut(s)	676
PshBI	ATTAAT	1 cut(s)	1749
Psp124BI	GAGCTC	2 cut(s)	669, 1746
Psp1406I	AACGTT	1 cut(s)	1364
Psp5II	RGGWCCY	1 cut(s)	676
Psp6I	CCWGG	4 cut(s)	183, 423, 807, 961
PspCI	CACGTG	1 cut(s)	1585
PspFI	CCCAGC	1 cut(s)	1568
PspGI	CCWGG	4 cut(s)	183, 423, 807, 961
PspOMI	GGGCCC	1 cut(s)	1712
PspPPI	RGGWCCY	1 cut(s)	676
PsuI	RGATCY	1 cut(s)	1072
PvuII	CAGCTG	2 cut(s)	719, 1433
RsaI	GTAC	8 cut(s)	534, 635, 728, 776, 1144, 1148, 1256, 1262
RsaNI	GTAC	8 cut(s)	533, 634, 727, 775, 1143, 1147, 1255, 1261
RseI	CAYNNNNRTG	1 cut(s)	1679
SacI	GAGCTC	2 cut(s)	669, 1746
SalI	GTCGAC	2 cut(s)	842, 1315
SapI	GCTCTTC	1 cut(s)	617
SaqAI	TTAA	3 cut(s)	872, 1611, 1749
SatI	GCNGC	9 cut(s)	39, 148, 252, 276, 420, 786, 1434, 1589, 1625
Sau3AI	GATC	3 cut(s)	1072, 1278, 1351
SchI	GAGTC	6 cut(s)	94, 192, 386, 912, 1223, 1529
ScrFI	CCNGG	7 cut(s)	185, 235, 425, 809, 963, 1489, 1546
SduI	GDGCHC	5 cut(s)	490, 669, 936, 1716, 1746
SfaNI	GCATC	3 cut(s)	471, 799, 1648
SfcI	CTRYAG	1 cut(s)	937
SfoI	GGCGCC	2 cut(s)	232, 967
SfuI	TTCGAA	1 cut(s)	381
SgrDI	CGTCGACG	1 cut(s)	842
SinI	GGWCC	4 cut(s)	333, 676, 828, 1105
SmiMI	CAYNNNNRTG	1 cut(s)	1679
SnaBI	TACGTA	1 cut(s)	1146
Sse9I	AATT	1 cut(s)	817
SspDI	GGCGCC	2 cut(s)	230, 965
SspI	AATATT	2 cut(s)	1000, 1609
SspMI	CTAG	3 cut(s)	191, 357, 411
SstI	GAGCTC	2 cut(s)	669, 1746
StyD4I	CCNGG	7 cut(s)	183, 233, 423, 807, 961, 1487, 1544
StyI	CCWWGG	1 cut(s)	528
TaaI	ACNGT	6 cut(s)	248, 554, 605, 1339, 1380, 1501
TaiI	ACGT	9 cut(s)	432, 755, 843, 1104, 1148, 1260, 1367, 1515, 1587
TaqII	GACCGA	2 cut(s)	845, 1175
TasI	AATT	1 cut(s)	817
TatI	WGTACW	2 cut(s)	726, 774
TauI	GCSGC	5 cut(s)	41, 254, 278, 1591, 1627
TfiI	GAWTC	2 cut(s)	858, 1204
Tru1I	TTAA	3 cut(s)	872, 1611, 1749
Tru9I	TTAA	3 cut(s)	872, 1611, 1749
TscAI	CASTG	3 cut(s)	111, 542, 1383
TseFI	GTSAC	4 cut(s)	164, 512, 1402, 1684
TseI	GCWGC	4 cut(s)	147, 419, 785, 1433
Tsp45I	GTSAC	4 cut(s)	164, 512, 1402, 1684
TspDTI	ATGAA	1 cut(s)	1332
TspGWI	ACGGA	2 cut(s)	942, 1190
TspRI	CASTG	3 cut(s)	111, 542, 1383
Van91I	CCANNNNNTGG	1 cut(s)	962
VpaK11BI	GGWCC	4 cut(s)	333, 676, 828, 1105
VspI	ATTAAT	1 cut(s)	1749
XceI	RCATGY	3 cut(s)	217, 727, 1114
XmiI	GTMKAC	2 cut(s)	843, 1316
XspI	CTAG	3 cut(s)	191, 357, 411
ZraI	GACGTC	1 cut(s)	841

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.