Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006919

Physical Location & Seq

Genomic Coordinates Forward (+)

44733185 .. 44734948

Gene Structure 1764 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1319800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGACTTCATCTCCCTTACCACCAACTTCTACAATGGCCGCCCTGCACTCCACCACCACAACCACCCTCTTCCGCTCTCCTTTATTCCCAAACAAGTCCCAGACTCCACTGCAACGAAAACCCAAACAATGCCTTGCGGGCAGAGTGCGCTGCAAAGCAACAAAAGGTGACAATGATAACCTAGACCAGGGCTTAGCAAGACTCGACAGGAGGAACATGCTGATAGGTTTAGGCACTGGGGGTCTCTACAGTGCGGCAGGAAACTCATTTGCTTTTGCAGCACCGGTATCCGCCCCAGACCTGACCACATGTGGCCCTGCCGACAAGCCAGACGGGTCCACCATCGATTGTTGCCCACCCATCACGACCACCATCATCGACTTCAAACTCCCCGACCGAGGCCCACTCCGCACAAGGATCGCTGCCCAGGACGTTGCAAAAAACCCTGCATACTTGGCTAAATACAAAAAGGCCATCGAGCTGATGCGGGCACTTCCAGATGACGACCCGCGCAGTCTCGTCCAACAGGCCAAAGTCCATTGCTCCTACTGCGACGGTGGATACCCACAAGTCGGATATTCAGATTTGGAGATCCAAGTTCACTTCTGTTGGCTATTCTTCCCGTTCCATCGTTGGTACCTCTACTTCTACGAGAAAATCATGGGCGAGCTCATTGGGGACCCAACCTTCGCCCTCCCCTTCTGGAACTGGGACGCGCCAGCTGGCATGTACATTCCTGAGATTTTCACCGATACGTCGTCATCCCTCTACGACCAGAACAGAAATACAGCGCATCAGCCCCCGAAGCTCCTGGACTTGAATTATGGCGGGACCGACGATGACGTCGACGACGCGACACGAATCAAAGAGAACCTAACAACGATGTACCAGCAGATGGTGTCGAAGGCCACTTCTCACAGACTATTCTACGGAGAGCCCTATAGCGCAGGGGACGAACCAGATCCTGGCGCCGGAAACATTGAGACCACTCCCCATAACAATATTCACCTTTGGGTTGGCGACCCAACCCAGACAAACGGGGAGGACATGGGGACCTTTTACTCTGCGGGGAGGGATCCGCTGTTTTACTCTCACCATTCCAACGTGGACCGCATGTGGTCTATATATAAAGATAAGTTGGGAGGTACGGACATAGAAAATACCGACTGGCTGGACGCAGAGTTCTTATTCTACGACGAGAAAAAGAATCTCGTGCGCGTCAAGGTTCGGGACTCGCTCGACACTAAAAAACTCGGGTACGTGTACGACGAGAAAGTCCCAATCCCATGGCTGAAGTCGAAGCCGACGGCTCGTAAGTCGACGAATAAGAGAAAGGCCACAGTTTCATCTTCTGATCTTACTACAACGTTCCCTGCTACATTGTCGGATACAATCAGCGTCGAGGTGACAAGGCCGTCTGCGACGAAGCGGACAGCTGCCCAGAAGAAGGCACATGACGAGGTGCTGGTGATTAAGGGGATTGAGTTTGCCGGGAATGAGCCTGTGAAGTTCGACGTGTATGTGAACGATGACGCGGAATCGCTGGCTGGGAAAGACAAGTCGGAGTTTGCTGGGAGTTTTGTTCACGTGCCGCATAAGCATAAGAAAAATATTAAGACGAACCTGCGACTGAGCATTATGAGCTTGTTGGAGGAGTTGGATGCGGAGACAGACAGCAGTTTGGTGGTGACTTTGGTGCCGAAAGTTGGGAAGGGGCCAATCACCATCGGAGGGTTTAGCATTGAGCTCATTAATACTACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

588

Amino Acids

65.18

Weight (kDa)

6.19

Isoelectric Point (pI)

39.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	172 - 378	2.9e-36	Common central domain of tyrosinase
PPO1_DWL	PF12142	384 - 436	7e-25	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	457 - 583	6e-47	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g04680
malus_domestica	MD05G1319800.v1.1 MD05G1320100.v1.1 MD05G1320200.v1.1 MD05G1320500.v1.1 MD05G1320800.v1.1 MD10G1298300.v1.1
prunus_persica	Prupe.4G041500_v2.0.a1 Prupe.4G041700_v2.0.a1
pyrus_communis	pycom05g29530 pycom05g29560 pycom10g24890 pycom10g24910 pycom10g25140
rosa_chinensis	RchiOBHm_Chr5g0007621
rosa_laevigata	RLG00000031479
rosa_multiflora	Rmu_co8439317.1_g000001 Rmu_sc0011744.1_g000006 Rmu_sc0021005.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068730
rosa_rugosa	Rorug04G0429400
rosa_samantha	Rh5AG057500 Rh5BG054400 Rh5CG064400 Rh5DG053600
rosa_wichuraiana	Rw5G006180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	842, 1274, 1414
AatII	GACGTC	1 cut(s)	846
Acc36I	ACCTGC	1 cut(s)	1632
Acc65I	GGTACC	1 cut(s)	636
AccB1I	GGYRCC	3 cut(s)	636, 968, 1696
AccB7I	CCANNNNNTGG	2 cut(s)	895, 965
AccBSI	CCGCTC	1 cut(s)	75
AccI	GTMKAC	2 cut(s)	846, 1319
AccII	CGCG	5 cut(s)	511, 716, 854, 1218, 1535
AclI	AACGTT	1 cut(s)	1367
AclWI	GGATC	5 cut(s)	425, 586, 956, 1070, 1083
AcoI	YGGCCR	1 cut(s)	36
AcuI	CTGAAG	1 cut(s)	1313
AcvI	CACGTG	1 cut(s)	1588
AcyI	GRCGYC	2 cut(s)	843, 969
AfaI	GTAC	6 cut(s)	638, 731, 887, 1147, 1259, 1265
AfiI	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
AflIII	ACRYGT	3 cut(s)	308, 1260, 1515
AgeI	ACCGGT	1 cut(s)	283
AgsI	TTSAA	2 cut(s)	385, 820
AjiI	CACGTC	1 cut(s)	1516
AjnI	CCWGG	4 cut(s)	186, 426, 810, 964
AloI	GAACNNNNNNTCC	2 cut(s)	1166, 1198
AluBI	AGCT	7 cut(s)	481, 670, 722, 808, 1436, 1644, 1747
AluI	AGCT	7 cut(s)	481, 670, 722, 808, 1436, 1644, 1747
Alw21I	GWGCWC	2 cut(s)	672, 1749
Alw26I	GTCTC	4 cut(s)	248, 521, 977, 1661
AlwI	GGATC	5 cut(s)	425, 586, 956, 1070, 1083
AlwNI	CAGNNNCTG	1 cut(s)	965
Ama87I	CYCGRG	1 cut(s)	1253
AoxI	GGCC	9 cut(s)	36, 313, 400, 471, 528, 906, 1335, 1412, 1715
ApeKI	GCWGC	4 cut(s)	150, 278, 422, 1436
ArsI	GACNNNNNNTTYG	2 cut(s)	671, 703
AseI	ATTAAT	1 cut(s)	1752
AsiGI	ACCGGT	1 cut(s)	283
Asp718I	GGTACC	1 cut(s)	636
AspLEI	GCGC	7 cut(s)	150, 513, 718, 793, 947, 971, 1218
AspS9I	GGNCC	8 cut(s)	314, 336, 401, 679, 831, 1053, 1108, 1715
AsuC2I	CCSGG	1 cut(s)	1492
AsuHPI	GGTGA	8 cut(s)	179, 739, 998, 1085, 1417, 1480, 1699, 1714
AvaI	CYCGRG	1 cut(s)	1253
AvaII	GGWCC	5 cut(s)	336, 679, 831, 1053, 1108
BaeGI	GKGCMC	1 cut(s)	493
BamHI	GGATCC	1 cut(s)	1075
BanI	GGYRCC	3 cut(s)	636, 968, 1696
BanII	GRGCYC	3 cut(s)	672, 939, 1749
BarI	GAAGNNNNNNTAC	2 cut(s)	629, 661
BauI	CACGAG	1 cut(s)	1211
BbrPI	CACGTG	1 cut(s)	1588
Bbv12I	GWGCWC	2 cut(s)	672, 1749
BbvI	GCAGC	4 cut(s)	137, 290, 409, 1423
BccI	CCATC	7 cut(s)	350, 368, 380, 482, 636, 889, 1733
BceAI	ACGGC	2 cut(s)	1323, 1399
BcgI	CGANNNNNNTGC	2 cut(s)	400, 434
BciT130I	CCWGG	4 cut(s)	188, 428, 812, 966
BciVI	GTATCC	3 cut(s)	298, 554, 1381
BcnI	CCSGG	1 cut(s)	1492
BcoDI	GTCTC	4 cut(s)	248, 521, 977, 1661
BfaI	CTAG	1 cut(s)	182
BfmI	CTRYAG	2 cut(s)	247, 940
BfoI	RGCGCY	1 cut(s)	972
BfuAI	ACCTGC	1 cut(s)	1632
BfuI	GTATCC	3 cut(s)	298, 554, 1381
BglI	GCCNNNNNGGC	1 cut(s)	138
BisI	GCNGC	7 cut(s)	39, 151, 255, 279, 423, 1437, 1592
BlpI	GCTNAGC	1 cut(s)	193
BlsI	GCNGC	7 cut(s)	40, 152, 256, 280, 424, 1438, 1593
Bme1390I	CCNGG	5 cut(s)	188, 428, 812, 966, 1492
Bme18I	GGWCC	5 cut(s)	336, 679, 831, 1053, 1108
BmeT110I	CYCGRG	1 cut(s)	1253
BmgBI	CACGTC	1 cut(s)	1516
BmgT120I	GGNCC	8 cut(s)	314, 336, 401, 679, 831, 1053, 1108, 1715
BmrFI	CCNGG	5 cut(s)	188, 428, 812, 966, 1492
BmrI	ACTGGG	2 cut(s)	246, 718
BmsI	GCATC	3 cut(s)	474, 802, 1651
BmuI	ACTGGG	2 cut(s)	246, 718
BplI	GAGNNNNNCTC	2 cut(s)	390, 422
Bpu1102I	GCTNAGC	1 cut(s)	193
BpuMI	CCSGG	1 cut(s)	1492
Bsa29I	ATCGAT	1 cut(s)	345
BsaAI	YACGTR	2 cut(s)	1261, 1588
BsaHI	GRCGYC	2 cut(s)	843, 969
BsaI	GGTCTC	2 cut(s)	248, 977
BsaJI	CCNNGG	4 cut(s)	187, 397, 426, 1286
BsaWI	WCCGGW	1 cut(s)	283
BsaXI	ACNNNNNCTCC	1 cut(s)	25
Bsc4I	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
Bse118I	RCCGGY	1 cut(s)	283
Bse1I	ACTGG	3 cut(s)	241, 713, 1172
Bse3DI	GCAATG	1 cut(s)	538
BseBI	CCWGG	4 cut(s)	188, 428, 812, 966
BseCI	ATCGAT	1 cut(s)	345
BseDI	CCNNGG	4 cut(s)	187, 397, 426, 1286
BseGI	GGATG	2 cut(s)	761, 1666
BseLI	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
BseMI	GCAATG	1 cut(s)	538
BseMII	CTCAG	2 cut(s)	729, 1622
BseNI	ACTGG	3 cut(s)	241, 713, 1172
BseRI	GAGGAG	1 cut(s)	1667
BseSI	GKGCMC	1 cut(s)	493
BseXI	GCAGC	4 cut(s)	137, 290, 409, 1423
BseYI	CCCAGC	2 cut(s)	1547, 1571
BsgI	GTGCAG	1 cut(s)	29
Bsh1236I	CGCG	5 cut(s)	511, 716, 854, 1218, 1535
Bsh1285I	CGRYCG	1 cut(s)	397
BshFI	GGCC	9 cut(s)	38, 315, 402, 473, 530, 908, 1337, 1414, 1717
BshNI	GGYRCC	3 cut(s)	636, 968, 1696
BshTI	ACCGGT	1 cut(s)	283
BshVI	ATCGAT	1 cut(s)	345
BsiEI	CGRYCG	1 cut(s)	397
BsiHKAI	GWGCWC	2 cut(s)	672, 1749
BsiHKCI	CYCGRG	1 cut(s)	1253
BsiSI	CCGG	3 cut(s)	284, 972, 1491
BslFI	GGGAC	8 cut(s)	82, 692, 725, 844, 965, 1066, 1244, 1262
BslI	CCNNNNNNNGG	9 cut(s)	187, 398, 572, 895, 965, 971, 1447, 1706, 1731
BsmAI	GTCTC	4 cut(s)	248, 521, 977, 1661
BsmFI	GGGAC	8 cut(s)	82, 692, 725, 844, 965, 1066, 1244, 1262
BsnI	GGCC	9 cut(s)	38, 315, 402, 473, 530, 908, 1337, 1414, 1717
Bso31I	GGTCTC	2 cut(s)	248, 977
BsoBI	CYCGRG	1 cut(s)	1253
Bsp1286I	GDGCHC	4 cut(s)	493, 672, 939, 1749
Bsp1407I	TGTACA	1 cut(s)	729
Bsp143I	GATC	5 cut(s)	417, 591, 961, 1075, 1354
Bsp1720I	GCTNAGC	1 cut(s)	193
Bsp19I	CCATGG	1 cut(s)	1286
BspANI	GGCC	9 cut(s)	38, 315, 402, 473, 530, 908, 1337, 1414, 1717
BspCNI	CTCAG	2 cut(s)	730, 1623
BspDI	ATCGAT	1 cut(s)	345
BspFNI	CGCG	5 cut(s)	511, 716, 854, 1218, 1535
BspMI	ACCTGC	1 cut(s)	1632
BspPI	GGATC	5 cut(s)	425, 586, 956, 1070, 1083
BspT107I	GGYRCC	3 cut(s)	636, 968, 1696
BspTNI	GGTCTC	2 cut(s)	248, 977
BsrBI	CCGCTC	1 cut(s)	75
BsrDI	GCAATG	1 cut(s)	538
BsrFI	RCCGGY	1 cut(s)	283
BsrGI	TGTACA	1 cut(s)	729
BsrI	ACTGG	3 cut(s)	241, 713, 1172
BssAI	RCCGGY	1 cut(s)	283
BssECI	CCNNGG	4 cut(s)	187, 397, 426, 1286
BssMI	GATC	5 cut(s)	417, 591, 961, 1075, 1354
BssNI	GRCGYC	2 cut(s)	843, 969
BssSI	CACGAG	1 cut(s)	1211
BssT1I	CCWWGG	1 cut(s)	1286
Bst2BI	CACGAG	1 cut(s)	1211
Bst2UI	CCWGG	4 cut(s)	188, 428, 812, 966
Bst4CI	ACNGT	3 cut(s)	251, 557, 1342
Bst6I	CTCTTC	1 cut(s)	74
BstACI	GRCGYC	2 cut(s)	843, 969
BstAUI	TGTACA	1 cut(s)	729
BstBAI	YACGTR	2 cut(s)	1261, 1588
BstC8I	GCNNGC	6 cut(s)	139, 489, 668, 720, 724, 1545
BstDEI	CTNAG	3 cut(s)	193, 738, 1631
BstDSI	CCRYGG	1 cut(s)	1286
BstENI	CCTNNNNNAGG	1 cut(s)	185
BstF5I	GGATG	2 cut(s)	761, 1666
BstFNI	CGCG	5 cut(s)	511, 716, 854, 1218, 1535
BstH2I	RGCGCY	1 cut(s)	972
BstHHI	GCGC	7 cut(s)	150, 513, 718, 793, 947, 971, 1218
BstKTI	GATC	5 cut(s)	420, 594, 964, 1078, 1357
BstMAI	GTCTC	4 cut(s)	248, 521, 977, 1661
BstMBI	GATC	5 cut(s)	417, 591, 961, 1075, 1354
BstMCI	CGRYCG	1 cut(s)	397
BstMWI	GCNNNNNNNGC	9 cut(s)	138, 147, 278, 408, 455, 549, 805, 1597, 1641
BstNI	CCWGG	4 cut(s)	188, 428, 812, 966
BstNSI	RCATGY	4 cut(s)	220, 312, 730, 1117
BstSCI	CCNGG	5 cut(s)	186, 426, 810, 964, 1490
BstSFI	CTRYAG	2 cut(s)	247, 940
BstSLI	GKGCMC	1 cut(s)	493
BstUI	CGCG	5 cut(s)	511, 716, 854, 1218, 1535
BstV1I	GCAGC	4 cut(s)	137, 290, 409, 1423
BstX2I	RGATCY	3 cut(s)	591, 961, 1075
BstXI	CCANNNNNNTGG	1 cut(s)	1287
BstYI	RGATCY	3 cut(s)	591, 961, 1075
Bsu15I	ATCGAT	1 cut(s)	345
BsuI	GTATCC	3 cut(s)	298, 554, 1381
BsuRI	GGCC	9 cut(s)	38, 315, 402, 473, 530, 908, 1337, 1414, 1717
BsuTUI	ATCGAT	1 cut(s)	345
BtgI	CCRYGG	1 cut(s)	1286
BtrI	CACGTC	1 cut(s)	1516
BtsCI	GGATG	2 cut(s)	761, 1666
BtsI	GCAGTG	1 cut(s)	107
BtsIMutI	CAGTG	3 cut(s)	107, 234, 256
BveI	ACCTGC	1 cut(s)	1632
Cac8I	GCNNGC	6 cut(s)	139, 489, 668, 720, 724, 1545
CaiI	CAGNNNCTG	1 cut(s)	965
CfoI	GCGC	7 cut(s)	150, 513, 718, 793, 947, 971, 1218
Cfr10I	RCCGGY	1 cut(s)	283
Cfr13I	GGNCC	8 cut(s)	314, 336, 401, 679, 831, 1053, 1108, 1715
ClaI	ATCGAT	1 cut(s)	345
CseI	GACGC	6 cut(s)	722, 860, 1184, 1207, 1387, 1541
Csp6I	GTAC	6 cut(s)	637, 730, 886, 1146, 1258, 1264
CspAI	ACCGGT	1 cut(s)	283
CviAII	CATG	8 cut(s)	217, 309, 661, 727, 1048, 1114, 1287, 1454
CviQI	GTAC	6 cut(s)	637, 730, 886, 1146, 1258, 1264
DdeI	CTNAG	3 cut(s)	193, 738, 1631
DinI	GGCGCC	1 cut(s)	970
DpnI	GATC	5 cut(s)	419, 593, 963, 1077, 1356
DpnII	GATC	5 cut(s)	417, 591, 961, 1075, 1354
DrdI	GACNNNNNNGTC	3 cut(s)	842, 1274, 1414
DseDI	GACNNNNNNGTC	3 cut(s)	842, 1274, 1414
EaeI	YGGCCR	1 cut(s)	36
Eam1104I	CTCTTC	1 cut(s)	74
EarI	CTCTTC	1 cut(s)	74
EciI	GGCGGA	1 cut(s)	280
Ecl136II	GAGCTC	2 cut(s)	670, 1747
Eco130I	CCWWGG	1 cut(s)	1286
Eco24I	GRGCYC	3 cut(s)	672, 939, 1749
Eco31I	GGTCTC	2 cut(s)	248, 977
Eco47I	GGWCC	5 cut(s)	336, 679, 831, 1053, 1108
Eco53kI	GAGCTC	2 cut(s)	670, 1747
Eco57I	CTGAAG	1 cut(s)	1313
Eco72I	CACGTG	1 cut(s)	1588
Eco88I	CYCGRG	1 cut(s)	1253
EcoICRI	GAGCTC	2 cut(s)	670, 1747
EcoNI	CCTNNNNNAGG	1 cut(s)	185
EcoO109I	RGGNCCY	2 cut(s)	679, 1053
EcoRII	CCWGG	4 cut(s)	186, 426, 810, 964
EcoT14I	CCWWGG	1 cut(s)	1286
EcoT38I	GRGCYC	3 cut(s)	672, 939, 1749
EgeI	GGCGCC	1 cut(s)	970
EheI	GGCGCC	1 cut(s)	970
ErhI	CCWWGG	1 cut(s)	1286
FaeI	CATG	8 cut(s)	220, 312, 664, 730, 1051, 1117, 1290, 1457
FaqI	GGGAC	8 cut(s)	82, 692, 725, 844, 965, 1066, 1244, 1262
FatI	CATG	8 cut(s)	216, 308, 660, 726, 1047, 1113, 1286, 1453
FauI	CCCGC	5 cut(s)	130, 480, 516, 821, 1060
FblI	GTMKAC	2 cut(s)	846, 1319
Fnu4HI	GCNGC	7 cut(s)	39, 151, 255, 279, 423, 1437, 1592
FokI	GGATG	2 cut(s)	748, 1673
FriOI	GRGCYC	3 cut(s)	672, 939, 1749
Fsp4HI	GCNGC	7 cut(s)	39, 151, 255, 279, 423, 1437, 1592
FspBI	CTAG	1 cut(s)	182
GlaI	GCGC	7 cut(s)	149, 512, 717, 792, 946, 970, 1217
GluI	GCNGC	7 cut(s)	39, 151, 255, 279, 423, 1437, 1592
GsaI	CCCAGC	2 cut(s)	1551, 1575
HaeII	RGCGCY	1 cut(s)	972
HaeIII	GGCC	9 cut(s)	38, 315, 402, 473, 530, 908, 1337, 1414, 1717
HapII	CCGG	3 cut(s)	284, 972, 1491
HgaI	GACGC	6 cut(s)	722, 860, 1184, 1207, 1387, 1541
HhaI	GCGC	7 cut(s)	150, 513, 718, 793, 947, 971, 1218
Hin1I	GRCGYC	2 cut(s)	843, 969
Hin1II	CATG	8 cut(s)	220, 312, 664, 730, 1051, 1117, 1290, 1457
Hin6I	GCGC	7 cut(s)	148, 511, 716, 791, 945, 969, 1216
HinP1I	GCGC	7 cut(s)	148, 511, 716, 791, 945, 969, 1216
HincII	GTYRAC	2 cut(s)	847, 1320
HindII	GTYRAC	2 cut(s)	847, 1320
HinfI	GANTC	6 cut(s)	103, 201, 861, 1207, 1232, 1538
HpaII	CCGG	3 cut(s)	284, 972, 1491
HphI	GGTGA	8 cut(s)	179, 739, 998, 1085, 1417, 1480, 1699, 1714
Hpy166II	GTNNAC	8 cut(s)	339, 601, 847, 1108, 1264, 1320, 1525, 1585
Hpy188I	TCNGA	6 cut(s)	575, 583, 1354, 1387, 1564, 1730
Hpy188III	TCNNGA	5 cut(s)	364, 497, 703, 737, 1229
Hpy8I	GTNNAC	8 cut(s)	339, 601, 847, 1108, 1264, 1320, 1525, 1585
HpyAV	CCTTC	5 cut(s)	697, 709, 898, 1441, 1705
HpyCH4III	ACNGT	3 cut(s)	251, 557, 1342
HpyCH4IV	ACGT	8 cut(s)	432, 755, 843, 1104, 1260, 1367, 1515, 1587
HpyCH4V	TGCA	6 cut(s)	46, 112, 153, 278, 437, 449
HpyF10VI	GCNNNNNNNGC	9 cut(s)	138, 147, 278, 408, 455, 549, 805, 1597, 1641
HpyF3I	CTNAG	3 cut(s)	193, 738, 1631
HpySE526I	ACGT	8 cut(s)	432, 755, 843, 1104, 1260, 1367, 1515, 1587
Hsp92I	GRCGYC	2 cut(s)	843, 969
Hsp92II	CATG	8 cut(s)	220, 312, 664, 730, 1051, 1117, 1290, 1457
HspAI	GCGC	7 cut(s)	148, 511, 716, 791, 945, 969, 1216
KasI	GGCGCC	1 cut(s)	968
KflI	GGGWCCC	1 cut(s)	679
KpnI	GGTACC	1 cut(s)	640
Kzo9I	GATC	5 cut(s)	417, 591, 961, 1075, 1354
LmnI	GCTCC	2 cut(s)	548, 813
Lsp1109I	GCAGC	4 cut(s)	137, 290, 409, 1423
LweI	GCATC	3 cut(s)	474, 802, 1651
MaeI	CTAG	1 cut(s)	182
MaeII	ACGT	8 cut(s)	432, 755, 843, 1104, 1260, 1367, 1515, 1587
MaeIII	GTNAC	3 cut(s)	167, 1405, 1687
MalI	GATC	5 cut(s)	419, 593, 963, 1077, 1356
MbiI	CCGCTC	1 cut(s)	75
MboI	GATC	5 cut(s)	417, 591, 961, 1075, 1354
MboII	GAAGA	4 cut(s)	61, 610, 1341, 1456
MflI	RGATCY	3 cut(s)	591, 961, 1075
MhlI	GDGCHC	4 cut(s)	493, 672, 939, 1749
MluCI	AATT	1 cut(s)	820
Mly113I	GGCGCC	1 cut(s)	969
MlyI	GAGTC	3 cut(s)	97, 195, 1226
MmeI	TCCRAC	7 cut(s)	547, 553, 1125, 1365, 1542, 1629, 1638
MseI	TTAA	3 cut(s)	1473, 1614, 1752
MspA1I	CMGCKG	3 cut(s)	722, 1081, 1436
MspI	CCGG	3 cut(s)	284, 972, 1491
MspR9I	CCNGG	5 cut(s)	188, 428, 812, 966, 1492
MvaI	CCWGG	4 cut(s)	188, 428, 812, 966
MvnI	CGCG	5 cut(s)	511, 716, 854, 1218, 1535
MwoI	GCNNNNNNNGC	9 cut(s)	138, 147, 278, 408, 455, 549, 805, 1597, 1641
NarI	GGCGCC	1 cut(s)	969
NciI	CCSGG	1 cut(s)	1492
NcoI	CCATGG	1 cut(s)	1286
NdeII	GATC	5 cut(s)	417, 591, 961, 1075, 1354
NlaIII	CATG	8 cut(s)	220, 312, 664, 730, 1051, 1117, 1290, 1457
NmuCI	GTSAC	3 cut(s)	167, 1405, 1687
NspI	RCATGY	4 cut(s)	220, 312, 730, 1117
PciI	ACATGT	1 cut(s)	308
PcsI	WCGNNNNNNNCGW	2 cut(s)	843, 1266
PfeI	GAWTC	3 cut(s)	861, 1207, 1538
PflMI	CCANNNNNTGG	2 cut(s)	895, 965
PfoI	TCCNGGA	1 cut(s)	810
PinAI	ACCGGT	1 cut(s)	283
PkrI	GCNGC	7 cut(s)	40, 152, 256, 280, 424, 1438, 1593
PleI	GAGTC	3 cut(s)	97, 195, 1226
PluTI	GGCGCC	1 cut(s)	972
PmaCI	CACGTG	1 cut(s)	1588
PmlI	CACGTG	1 cut(s)	1588
PpsI	GAGTC	3 cut(s)	97, 195, 1226
Ppu21I	YACGTR	2 cut(s)	1261, 1588
PpuMI	RGGWCCY	2 cut(s)	679, 1053
PscI	ACATGT	1 cut(s)	308
PshBI	ATTAAT	1 cut(s)	1752
Psp124BI	GAGCTC	2 cut(s)	672, 1749
Psp1406I	AACGTT	1 cut(s)	1367
Psp5II	RGGWCCY	2 cut(s)	679, 1053
Psp6I	CCWGG	4 cut(s)	186, 426, 810, 964
PspCI	CACGTG	1 cut(s)	1588
PspFI	CCCAGC	2 cut(s)	1547, 1571
PspGI	CCWGG	4 cut(s)	186, 426, 810, 964
PspPI	GGNCC	8 cut(s)	314, 336, 401, 679, 831, 1053, 1108, 1715
PspPPI	RGGWCCY	2 cut(s)	679, 1053
PstNI	CAGNNNCTG	1 cut(s)	965
PsuI	RGATCY	3 cut(s)	591, 961, 1075
PvuII	CAGCTG	2 cut(s)	722, 1436
RsaI	GTAC	6 cut(s)	638, 731, 887, 1147, 1259, 1265
RsaNI	GTAC	6 cut(s)	637, 730, 886, 1146, 1258, 1264
SacI	GAGCTC	2 cut(s)	672, 1749
SalI	GTCGAC	2 cut(s)	845, 1318
SaqAI	TTAA	3 cut(s)	1473, 1614, 1752
SatI	GCNGC	7 cut(s)	39, 151, 255, 279, 423, 1437, 1592
Sau3AI	GATC	5 cut(s)	417, 591, 961, 1075, 1354
Sau96I	GGNCC	8 cut(s)	314, 336, 401, 679, 831, 1053, 1108, 1715
SchI	GAGTC	3 cut(s)	97, 195, 1226
ScrFI	CCNGG	5 cut(s)	188, 428, 812, 966, 1492
SduI	GDGCHC	4 cut(s)	493, 672, 939, 1749
SfaNI	GCATC	3 cut(s)	474, 802, 1651
SfcI	CTRYAG	2 cut(s)	247, 940
SfoI	GGCGCC	1 cut(s)	970
SgrDI	CGTCGACG	1 cut(s)	845
SinI	GGWCC	5 cut(s)	336, 679, 831, 1053, 1108
Sse9I	AATT	1 cut(s)	820
SspDI	GGCGCC	1 cut(s)	968
SspI	AATATT	2 cut(s)	1003, 1612
SspMI	CTAG	1 cut(s)	182
SstI	GAGCTC	2 cut(s)	672, 1749
StyD4I	CCNGG	5 cut(s)	186, 426, 810, 964, 1490
StyI	CCWWGG	1 cut(s)	1286
TaaI	ACNGT	3 cut(s)	251, 557, 1342
TaiI	ACGT	8 cut(s)	435, 758, 846, 1107, 1263, 1370, 1518, 1590
TaqII	GACCGA	2 cut(s)	411, 848
TasI	AATT	1 cut(s)	820
TatI	WGTACW	1 cut(s)	729
TauI	GCSGC	3 cut(s)	41, 257, 1594
TfiI	GAWTC	3 cut(s)	861, 1207, 1538
Tru1I	TTAA	3 cut(s)	1473, 1614, 1752
Tru9I	TTAA	3 cut(s)	1473, 1614, 1752
TscAI	CASTG	3 cut(s)	114, 241, 256
TseFI	GTSAC	3 cut(s)	167, 1405, 1687
TseI	GCWGC	4 cut(s)	150, 278, 422, 1436
Tsp45I	GTSAC	3 cut(s)	167, 1405, 1687
TspDTI	ATGAA	1 cut(s)	1335
TspGWI	ACGGA	2 cut(s)	945, 1163
TspRI	CASTG	3 cut(s)	114, 241, 256
Van91I	CCANNNNNTGG	2 cut(s)	895, 965
VpaK11BI	GGWCC	5 cut(s)	336, 679, 831, 1053, 1108
VspI	ATTAAT	1 cut(s)	1752
XagI	CCTNNNNNAGG	1 cut(s)	185
XceI	RCATGY	4 cut(s)	220, 312, 730, 1117
XmiI	GTMKAC	2 cut(s)	846, 1319
XspI	CTAG	1 cut(s)	182
ZraI	GACGTC	1 cut(s)	844

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.