FvH4_3g05310

Leucine Rich Repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016779
Physical Location & Seq
Forward (+)
3095925 .. 3097728
1804 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05310.t1

Sequence Viewer

Length: 1539 bp
ATGGCATTTATACTTGAATTGGGCACAGCTAAAACCTTTAACTCACTCTTCGAACTTTTGGATACCCAGTTTTTCACTTGTGGGTTTTGCTGTGTTTATTGGTATCTTTGTGAGTTTGTTCTCATGGCCTTTTCTTCTTCTGTGTGTCTTTTCCTTGTTGGGTTTCTTCTTTTTGCAGGCAAGGGGTTTGCTGAAATGCCTATCTCAGATGATGAGGTTCCATCAGAGATGGAAAAGGAGGAGCTTCTGGGGATTTTTGAGGTAATGGGTGCCCTTCTTGAGGACTCTGATTGGGCTCTAGAGCACCCACAACCATGTACTGAGACTCCATGGCCTGGTGTTGAATGTGAGATTGGGGATGAAGACCCTCCAATGTTTCATGTCACAAAGATACATATTGGCCCTGATATTCTCACCCCTCCCTGCAAATCCTCTGCTTCTTTATCAGAGTCCCTTCTCAAATTACCTTACTTACAAACCCTTTCAATATTTAGTTGCTTTACAACATCACAAGTCACTCTTTCTCCAAAGCTCTTTGGTGCATTGTCTTCCTTGGAACACCTAACCCTTGTGTCCAATCCAACTCTCTCAGGTGAAATTCCCTCAAGTTTGTCCCAAATTTCTAGCTTAAGGATACTGAGTTTGTCACAGAACAATTTACAAGGAAAAGTCCCTAGCAAGATTGGTAGTTTGGTCAGCTTAGAGCAACTTGACTTGAGTTACAACAACTTGAGTGGTCAAATACCAGAAGAAATTGGAGGGTTGGAGAGTTTGACCATTTTGGATTTAAGCCGGAATGTTCTTGAAGGTCAGGTACCCTCCTCTATTGGTCAGCTTAAACTCCTCCAAAAGATTGATTTCAGCTCAAACGGACTTAGAGGTATGTTGCCTCCAGATATAGGGACGCTCAACAAGTTGGTCTTGCTTGATCTAAGTCACAACTTAATCAATGGGCCAATCCCTGAGACCCTTTCAGGGATGCAGCATTTAGAGTACATGGTAGCTGATAGCAACCCAATCAATACAGAAGTTCCTAACTTTGTTGGGAAGCTCATGAACCTCAAAACACTGAGCTTTTCAGAATGTGGATTGATAGGGCCATTACCCAACTTCCTCTCTTCCTTGAAGAATCTTACTGCTCTTTCTTTAGATAACAATAGCCTCACTGGGACTGTTCCTCCAAATTTGGAGACCCTTCCAAGTCTGGATTTGCTGAATTTGAGCAACAATCAACTAAGTGGTGAGCTGACACTTTCAGAAGATTTCATTGAGAGGCTTGGGAAGAGGTTGGTGCTAAGAGGAAACAATGGGTTATGTACAAGCAATCCACTTTACAAGAAGGAGAACATTTCTGGCTATGTGATAGCTCCTTTGTGTTTGAATACAAGAGGGCTGGGAGATCAAAACCCGGAGTCTGTGAGAATGAAGCCATTGGATCAAAACCCTGTTCAGGAAAATTCATCTGCAGGTTCTTCATGGTCAAATGTGAAACTGAATTCATTTGGTTTTGTTATTTGTTGCTTGAGTCTGTTATTGTAA

Protein Analysis

513

Amino Acids

55.72

Weight (kDa)

4.55

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 202 - 244 6.5e-06 Leucine rich repeat
LRR_14 PF23598 202 - 309 2.4e-11 Leucine-rich repeat region
LRR_8 PF13855 209 - 268 3.1e-09 Leucine rich repeat
LRR_4 PF12799 209 - 248 1e-05 Leucine Rich repeats (2 copies)
LRR_14 PF23598 270 - 333 6.4e-06 Leucine-rich repeat region
LRR_14 PF23598 325 - 428 1.9e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016779)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05310
malus_domestica MD05G1281800.v1.1
prunus_persica Prupe.4G082700_v2.0.a1
pyrus_communis pycom05g26170
rosa_chinensis RchiOBHm_Chr5g0014421
rosa_laevigata RLG00000032107
rosa_multiflora Rmu_ssc0000422.1_g000037
rosa_roxburghii Rroxscaffold_1G00062160
rosa_rugosa Rorug05G0017900
rosa_samantha Rh5AG112800 Rh5BG109600 Rh5CG121400 Rh5DG108600
rosa_wichuraiana Rw5G009790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1457
Acc65I GGTACC 1 cut(s) 814
AccB1I GGYRCC 2 cut(s) 269, 814
AccB7I CCANNNNNTGG 1 cut(s) 335
AclWI GGATC 1 cut(s) 1443
AcsI RAATTY 6 cut(s) 597, 618, 1183, 1216, 1456, 1495
AfaI GTAC 4 cut(s) 319, 816, 995, 1318
AfiI CCNNNNNNNGG 5 cut(s) 280, 335, 899, 975, 1450
AflII CTTAAG 1 cut(s) 628
AgsI TTSAA 6 cut(s) 17, 344, 486, 806, 1126, 1381
AjnI CCWGG 1 cut(s) 334
AjuI GAANNNNNNNTTGG 2 cut(s) 336, 368
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 3 cut(s) 317, 959, 1184
AlwI GGATC 1 cut(s) 1443
AoxI GGCC 5 cut(s) 126, 332, 400, 953, 1097
ApeKI GCWGC 1 cut(s) 982
ApoI RAATTY 6 cut(s) 597, 618, 1183, 1216, 1456, 1495
Asp718I GGTACC 1 cut(s) 814
AspS9I GGNCC 3 cut(s) 401, 953, 1097
AsuC2I CCSGG 1 cut(s) 1409
AsuHPI GGTGA 3 cut(s) 406, 605, 1253
AsuII TTCGAA 1 cut(s) 51
BaeGI GKGCMC 2 cut(s) 26, 274
BanI GGYRCC 2 cut(s) 269, 814
BanII GRGCYC 1 cut(s) 298
BarI GAAGNNNNNNTAC 2 cut(s) 798, 830
BbsI GAAGAC 2 cut(s) 369, 540
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 994
BccI CCATC 2 cut(s) 223, 229
BciT130I CCWGG 1 cut(s) 336
BciVI GTATCC 2 cut(s) 55, 627
BcnI CCSGG 1 cut(s) 1409
BcoDI GTCTC 3 cut(s) 317, 959, 1184
BfaI CTAG 3 cut(s) 299, 624, 675
BfmI CTRYAG 1 cut(s) 1464
BfrI CTTAAG 1 cut(s) 628
BfuAI ACCTGC 1 cut(s) 1457
BfuI GTATCC 2 cut(s) 55, 627
BisI GCNGC 1 cut(s) 983
BlsI GCNGC 1 cut(s) 984
Bme1390I CCNGG 2 cut(s) 336, 1409
BmgT120I GGNCC 3 cut(s) 401, 953, 1097
BmiI GGNNCC 3 cut(s) 219, 271, 816
BmrFI CCNGG 2 cut(s) 336, 1409
BmrI ACTGGG 2 cut(s) 61, 1176
BmsI GCATC 1 cut(s) 969
BmuI ACTGGG 2 cut(s) 61, 1176
BpiI GAAGAC 2 cut(s) 369, 540
BpmI CTGGAG 1 cut(s) 876
Bpu14I TTCGAA 1 cut(s) 51
BpuEI CTTGAG 4 cut(s) 299, 589, 736, 751
BpuMI CCSGG 1 cut(s) 1409
BsaI GGTCTC 2 cut(s) 959, 1184
BsaJI CCNNGG 2 cut(s) 329, 552
BsaXI ACNNNNNCTCC 6 cut(s) 310, 340, 508, 538, 1162, 1192
Bsc4I CCNNNNNNNGG 5 cut(s) 280, 335, 899, 975, 1450
Bse1I ACTGG 2 cut(s) 67, 1171
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 329, 552
BseGI GGATG 2 cut(s) 364, 984
BseLI CCNNNNNNNGG 5 cut(s) 280, 335, 899, 975, 1450
BseMII CTCAG 6 cut(s) 219, 312, 603, 629, 954, 1061
BseNI ACTGG 2 cut(s) 67, 1171
BseRI GAGGAG 3 cut(s) 254, 811, 833
BseSI GKGCMC 2 cut(s) 26, 274
BseXI GCAGC 1 cut(s) 994
BseYI CCCAGC 1 cut(s) 1393
BshFI GGCC 5 cut(s) 128, 334, 402, 955, 1099
BshNI GGYRCC 2 cut(s) 269, 814
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 2 cut(s) 793, 1409
BslFI GGGAC 5 cut(s) 436, 598, 656, 916, 1183
BslI CCNNNNNNNGG 5 cut(s) 280, 335, 899, 975, 1450
BsmAI GTCTC 3 cut(s) 317, 959, 1184
BsmFI GGGAC 5 cut(s) 436, 598, 656, 916, 1183
BsnI GGCC 5 cut(s) 128, 334, 402, 955, 1099
Bso31I GGTCTC 2 cut(s) 959, 1184
Bsp119I TTCGAA 1 cut(s) 51
Bsp1286I GDGCHC 4 cut(s) 26, 274, 298, 306
Bsp1407I TGTACA 1 cut(s) 1316
Bsp143I GATC 3 cut(s) 928, 1399, 1435
Bsp19I CCATGG 1 cut(s) 329
BspANI GGCC 5 cut(s) 128, 334, 402, 955, 1099
BspCNI CTCAG 6 cut(s) 218, 313, 602, 630, 955, 1062
BspHI TCATGA 1 cut(s) 1053
BspLI GGNNCC 3 cut(s) 219, 271, 816
BspMAI CTGCAG 1 cut(s) 1468
BspMI ACCTGC 1 cut(s) 1457
BspPI GGATC 1 cut(s) 1443
BspT104I TTCGAA 1 cut(s) 51
BspT107I GGYRCC 2 cut(s) 269, 814
BspTI CTTAAG 1 cut(s) 628
BspTNI GGTCTC 2 cut(s) 959, 1184
BsrGI TGTACA 1 cut(s) 1316
BsrI ACTGG 2 cut(s) 67, 1171
BssECI CCNNGG 2 cut(s) 329, 552
BssMI GATC 3 cut(s) 928, 1399, 1435
BssT1I CCWWGG 2 cut(s) 329, 552
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 1174
Bst6I CTCTTC 3 cut(s) 53, 1123, 1277
BstAFI CTTAAG 1 cut(s) 628
BstAUI TGTACA 1 cut(s) 1316
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 178
BstDSI CCRYGG 1 cut(s) 329
BstENI CCTNNNNNAGG 1 cut(s) 278
BstF5I GGATG 2 cut(s) 364, 984
BstKTI GATC 3 cut(s) 931, 1402, 1438
BstMAI GTCTC 3 cut(s) 317, 959, 1184
BstMBI GATC 3 cut(s) 928, 1399, 1435
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 2 cut(s) 334, 1407
BstSFI CTRYAG 1 cut(s) 1464
BstSLI GKGCMC 2 cut(s) 26, 274
BstV1I GCAGC 1 cut(s) 994
BstV2I GAAGAC 2 cut(s) 369, 540
BsuI GTATCC 2 cut(s) 55, 627
BsuRI GGCC 5 cut(s) 128, 334, 402, 955, 1099
BtgI CCRYGG 1 cut(s) 329
BtsCI GGATG 2 cut(s) 364, 984
BtsIMutI CAGTG 2 cut(s) 1067, 1164
BveI ACCTGC 1 cut(s) 1457
Cac8I GCNNGC 1 cut(s) 178
CciI TCATGA 1 cut(s) 1053
Cfr13I GGNCC 3 cut(s) 401, 953, 1097
CseI GACGC 1 cut(s) 913
Csp6I GTAC 4 cut(s) 318, 815, 994, 1317
CspCI CAANNNNNGTGG 2 cut(s) 715, 750
CviAII CATG 7 cut(s) 124, 315, 330, 380, 997, 1054, 1476
CviQI GTAC 4 cut(s) 318, 815, 994, 1317
DpnI GATC 3 cut(s) 930, 1401, 1437
DpnII GATC 3 cut(s) 928, 1399, 1435
Eam1104I CTCTTC 3 cut(s) 53, 1123, 1277
EarI CTCTTC 3 cut(s) 53, 1123, 1277
Eco130I CCWWGG 2 cut(s) 329, 552
Eco24I GRGCYC 1 cut(s) 298
Eco31I GGTCTC 2 cut(s) 959, 1184
EcoNI CCTNNNNNAGG 1 cut(s) 278
EcoRI GAATTC 1 cut(s) 1495
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 2 cut(s) 329, 552
EcoT38I GRGCYC 1 cut(s) 298
ErhI CCWWGG 2 cut(s) 329, 552
FaeI CATG 7 cut(s) 127, 318, 333, 383, 1000, 1057, 1479
FalI AAGNNNNNCTT 4 cut(s) 504, 536, 905, 937
FaqI GGGAC 5 cut(s) 436, 598, 656, 916, 1183
FatI CATG 7 cut(s) 123, 314, 329, 379, 996, 1053, 1475
Fnu4HI GCNGC 1 cut(s) 983
FokI GGATG 2 cut(s) 371, 991
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 1 cut(s) 983
FspBI CTAG 3 cut(s) 299, 624, 675
GluI GCNGC 1 cut(s) 983
GsaI CCCAGC 1 cut(s) 1397
GsuI CTGGAG 1 cut(s) 876
HaeIII GGCC 5 cut(s) 128, 334, 402, 955, 1099
HapII CCGG 2 cut(s) 793, 1409
HgaI GACGC 1 cut(s) 913
Hin1II CATG 7 cut(s) 127, 318, 333, 383, 1000, 1057, 1479
HinfI GANTC 6 cut(s) 284, 325, 449, 1129, 1412, 1525
HpaII CCGG 2 cut(s) 793, 1409
HphI GGTGA 3 cut(s) 406, 605, 1253
Hpy188I TCNGA 6 cut(s) 208, 226, 289, 448, 1081, 1258
Hpy188III TCNNGA 7 cut(s) 278, 299, 803, 893, 1054, 1205, 1451
HpyAV CCTTC 5 cut(s) 284, 464, 800, 1205, 1333
HpyCH4III ACNGT 1 cut(s) 1174
HpyCH4V TGCA 5 cut(s) 176, 426, 542, 982, 1466
Hsp92II CATG 7 cut(s) 127, 318, 333, 383, 1000, 1057, 1479
KpnI GGTACC 1 cut(s) 818
Kzo9I GATC 3 cut(s) 928, 1399, 1435
LmnI GCTCC 2 cut(s) 241, 1372
Lsp1109I GCAGC 1 cut(s) 994
LweI GCATC 1 cut(s) 969
MaeI CTAG 3 cut(s) 299, 624, 675
MaeIII GTNAC 5 cut(s) 382, 514, 645, 719, 935
MalI GATC 3 cut(s) 930, 1401, 1437
MboI GATC 3 cut(s) 928, 1399, 1435
MhlI GDGCHC 4 cut(s) 26, 274, 298, 306
MlyI GAGTC 5 cut(s) 278, 319, 458, 1421, 1534
MmeI TCCRAC 2 cut(s) 605, 744
MseI TTAA 5 cut(s) 39, 629, 788, 837, 944
MslI CAYNNNNRTG 1 cut(s) 313
MspCI CTTAAG 1 cut(s) 628
MspI CCGG 2 cut(s) 793, 1409
MspR9I CCNGG 2 cut(s) 336, 1409
MvaI CCWGG 1 cut(s) 336
NciI CCSGG 1 cut(s) 1409
NcoI CCATGG 1 cut(s) 329
NdeII GATC 3 cut(s) 928, 1399, 1435
NlaIII CATG 7 cut(s) 127, 318, 333, 383, 1000, 1057, 1479
NlaIV GGNNCC 3 cut(s) 219, 271, 816
NmuCI GTSAC 4 cut(s) 382, 514, 645, 935
NspV TTCGAA 1 cut(s) 51
PagI TCATGA 1 cut(s) 1053
PfeI GAWTC 1 cut(s) 1129
PflMI CCANNNNNTGG 1 cut(s) 335
PkrI GCNGC 1 cut(s) 984
PleI GAGTC 5 cut(s) 278, 319, 457, 1420, 1533
PpsI GAGTC 5 cut(s) 278, 319, 457, 1420, 1533
Psp6I CCWGG 1 cut(s) 334
PspFI CCCAGC 1 cut(s) 1393
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 3 cut(s) 219, 271, 816
PspPI GGNCC 3 cut(s) 401, 953, 1097
PstI CTGCAG 1 cut(s) 1468
RsaI GTAC 4 cut(s) 319, 816, 995, 1318
RsaNI GTAC 4 cut(s) 318, 815, 994, 1317
RseI CAYNNNNRTG 1 cut(s) 313
SaqAI TTAA 5 cut(s) 39, 629, 788, 837, 944
SatI GCNGC 1 cut(s) 983
Sau3AI GATC 3 cut(s) 928, 1399, 1435
Sau96I GGNCC 3 cut(s) 401, 953, 1097
SchI GAGTC 5 cut(s) 278, 319, 458, 1421, 1534
ScrFI CCNGG 2 cut(s) 336, 1409
SduI GDGCHC 4 cut(s) 26, 274, 298, 306
SfaNI GCATC 1 cut(s) 969
SfcI CTRYAG 1 cut(s) 1464
SfuI TTCGAA 1 cut(s) 51
SmiMI CAYNNNNRTG 1 cut(s) 313
SmlI CTYRAG 6 cut(s) 278, 604, 628, 715, 730, 1522
SmoI CTYRAG 6 cut(s) 278, 604, 628, 715, 730, 1522
SspI AATATT 1 cut(s) 489
SspMI CTAG 3 cut(s) 299, 624, 675
StyD4I CCNGG 2 cut(s) 334, 1407
StyI CCWWGG 2 cut(s) 329, 552
TaaI ACNGT 1 cut(s) 1174
TaqI TCGA 1 cut(s) 51
TatI WGTACW 3 cut(s) 317, 993, 1316
TfiI GAWTC 1 cut(s) 1129
Tru1I TTAA 5 cut(s) 39, 629, 788, 837, 944
Tru9I TTAA 5 cut(s) 39, 629, 788, 837, 944
TscAI CASTG 2 cut(s) 1074, 1171
TseFI GTSAC 4 cut(s) 382, 514, 645, 935
TseI GCWGC 1 cut(s) 982
Tsp45I GTSAC 4 cut(s) 382, 514, 645, 935
TspDTI ATGAA 8 cut(s) 368, 375, 1070, 1255, 1439, 1449, 1464, 1488
TspGWI ACGGA 1 cut(s) 885
TspRI CASTG 2 cut(s) 1074, 1171
Van91I CCANNNNNTGG 1 cut(s) 335
Vha464I CTTAAG 1 cut(s) 628
XagI CCTNNNNNAGG 1 cut(s) 278
XapI RAATTY 6 cut(s) 597, 618, 1183, 1216, 1456, 1495
XbaI TCTAGA 1 cut(s) 298
XspI CTAG 3 cut(s) 299, 624, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.