Rh5AG112800

Leucine Rich Repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0016779
Physical Location & Seq
Reverse (-)
10401065 .. 10403391
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG112800.1

Sequence Viewer

Length: 1437 bp
ATGGCTTTTTCTTCTTTTGTGTGTCTTTTCCTTGTTGGGTTTCTTGTTTTTGCAGGTAAGTTGCTTGCTGAAATGCCTGACTCAGATGATGAGGTTCCACTAGAGATGGAAAAGAAGGAGCTTTTGGGGATTTTTGAGGTAATGGGTGCCCTTCTTGAGGACTCTGATTGGCCTCTAGAGCACCCACAACCATGTACTGAGACTCAATGGCCTGGTGTTCAATGTGAGATTGGGGACGAAGACCCTCCAATGTTTCACGTCACAAAGATACACATTGGCCCTGATATTCTCACCCCTCCTTGCAAATCATCTGCTACTTTATCAGAGTCCCTTCTCAAACTACCTTACTTGAAAACCCTCTCAATATTTAGTTGCTTTTCAACATCACAAGTCACTCTTTCTCCAAAGCTCTTTGGTGCATTGTCTTCCTTGGAACACCTAGCCCTTGTGTCCAATCCAACTCTCTCAGGTGAAATTCCCTCAAGTTTAGCCCAAATTTCCAGCTTAAGGGTTCTGACTTTGTCACAGAACAATTTACAAGGAAAAGTCCCTATTCAGATTGGTGCTTTGGTCAGCTTAGAGCAACTTGACTTGAGTTACAACAACTTGAGTGGTCAAATACCAGACGAAATTGGAGGGTTGGAGAGTTTGACCATTTTGGATTTAAGTCAGAATGTTCTTGAAGGTCAGCTGCCCTCCTCTTTTGGTCAGCTTCAACTCCTCCAAAAGATTGATTTAAGCTCAAACAGGCTTGGAGGTATGTTGCCTCCAGATATAGGGAAGCTCAACAGGTTGGTTTTGCTTGATCTGAGTCACAACTTAATAAATGGGCCAATCCCTGAAACCCTTTCAGGTTTGCAGCATTTAGAGTACATGGTAGCTGATAGCAACCCAATCAAGACAGAAGTTCCTAACTTTGTTGGGAAGCTCATGAATCTCAAAACACTCAGCTTTTCAGAATGTGGGTTGATAGGCCCATTACCCAACTTTCTCTCTTCCTTGAAGAATCTCACTGCTCTTTGTCTAAGCAACAATAGCCTCACTGGGACTGTTCGTCCAAATTTGGCCACTCTTCCAAGTCTTGATTTACTAAATTTGAGCAACAATCAGTTAAGTGGTGAGCTGACACTTCCAGAAGATTTCATTGAGAGGCTTGGAAAGAGGTTGGTGCTAAGAGGAAACAATGGGCTATGTACAAGCAATCCACTCAACAAGAAGGAGAATATTTCTAGCCATGTGATAGCCCCTCTGTGTTTGAATCCAAGAGGGCCCAGAGATGACCAGACCTTGGCTGGTGAACACCCAACTAAGTCTGAGAGAGTGAAGCCATTGGATCAAGACCCTGTGCAGGAAAGTTCATCTACAGGTTCTTCATGGTCACATGTAAAACTGAATTCATTTGGTTTTGTTTTTTGTTTGTTGAGTATGTTATTGTAA

Protein Analysis

478

Amino Acids

51.88

Weight (kDa)

4.88

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 143 - 203 1.4e-06 Leucine rich repeat
LRR_14 PF23598 161 - 245 8.8e-10 Leucine-rich repeat region
LRR_8 PF13855 168 - 227 9e-09 Leucine rich repeat
LRR_4 PF12799 168 - 208 8.8e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 192 - 251 4.7e-08 Leucine rich repeat
LRR_14 PF23598 253 - 340 3e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016779)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05310
malus_domestica MD05G1281800.v1.1
prunus_persica Prupe.4G082700_v2.0.a1
pyrus_communis pycom05g26170
rosa_chinensis RchiOBHm_Chr5g0014421
rosa_laevigata RLG00000032107
rosa_multiflora Rmu_ssc0000422.1_g000037
rosa_roxburghii Rroxscaffold_1G00062160
rosa_rugosa Rorug05G0017900
rosa_samantha Rh5AG112800 Rh5BG109600 Rh5CG121400 Rh5DG108600
rosa_wichuraiana Rw5G009790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 1288
AclWI GGATC 1 cut(s) 1341
AcoI YGGCCR 1 cut(s) 1065
AcsI RAATTY 5 cut(s) 474, 495, 1060, 1093, 1393
AfaI GTAC 3 cut(s) 196, 872, 1195
AfiI CCNNNNNNNGG 5 cut(s) 157, 507, 776, 1288, 1348
AflII CTTAAG 1 cut(s) 505
AflIII ACRYGT 1 cut(s) 1381
AgsI TTSAA 7 cut(s) 221, 352, 381, 683, 716, 1003, 1258
AhdI GACNNNNNGTC 1 cut(s) 1053
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 1 cut(s) 211
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 1 cut(s) 1341
AoxI GGCC 7 cut(s) 170, 209, 277, 830, 973, 1065, 1268
ApaI GGGCCC 1 cut(s) 1272
ApeKI GCWGC 2 cut(s) 691, 859
ApoI RAATTY 5 cut(s) 474, 495, 1060, 1093, 1393
AspS9I GGNCC 5 cut(s) 278, 830, 974, 1268, 1269
AsuHPI GGTGA 4 cut(s) 283, 482, 1130, 1307
BaeGI GKGCMC 2 cut(s) 151, 1272
BalI TGGCCA 1 cut(s) 1067
BanI GGYRCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 1272
BbsI GAAGAC 2 cut(s) 246, 417
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 2 cut(s) 678, 871
BccI CCATC 1 cut(s) 100
BciT130I CCWGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 4 cut(s) 101, 176, 440, 1230
BfmI CTRYAG 1 cut(s) 1362
BfrI CTTAAG 1 cut(s) 505
BfuAI ACCTGC 1 cut(s) 44
BisI GCNGC 2 cut(s) 692, 860
BlsI GCNGC 2 cut(s) 693, 861
Bme1390I CCNGG 1 cut(s) 213
BmeRI GACNNNNNGTC 1 cut(s) 1053
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 5 cut(s) 278, 830, 974, 1268, 1269
BmiI GGNNCC 3 cut(s) 96, 148, 1270
BmrFI CCNGG 1 cut(s) 213
BmrI ACTGGG 1 cut(s) 1053
BmuI ACTGGG 1 cut(s) 1053
BpiI GAAGAC 2 cut(s) 246, 417
BpmI CTGGAG 1 cut(s) 753
BpuEI CTTGAG 4 cut(s) 176, 466, 613, 628
BsaJI CCNNGG 2 cut(s) 429, 1287
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
Bsc4I CCNNNNNNNGG 5 cut(s) 157, 507, 776, 1288, 1348
Bse1I ACTGG 1 cut(s) 1048
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 429, 1287
BseLI CCNNNNNNNGG 5 cut(s) 157, 507, 776, 1288, 1348
BseMII CTCAG 6 cut(s) 96, 189, 480, 800, 961, 1305
BseNI ACTGG 1 cut(s) 1048
BseRI GAGGAG 2 cut(s) 688, 710
BseSI GKGCMC 2 cut(s) 151, 1272
BseXI GCAGC 2 cut(s) 678, 871
BsgI GTGCAG 1 cut(s) 1367
BshFI GGCC 7 cut(s) 172, 211, 279, 832, 975, 1067, 1270
BshNI GGYRCC 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 183
BslFI GGGAC 4 cut(s) 248, 313, 533, 1060
BslI CCNNNNNNNGG 5 cut(s) 157, 507, 776, 1288, 1348
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 4 cut(s) 248, 313, 533, 1060
BsnI GGCC 7 cut(s) 172, 211, 279, 832, 975, 1067, 1270
Bsp120I GGGCCC 1 cut(s) 1268
Bsp1286I GDGCHC 3 cut(s) 151, 183, 1272
Bsp1407I TGTACA 1 cut(s) 1193
Bsp143I GATC 2 cut(s) 805, 1333
BspANI GGCC 7 cut(s) 172, 211, 279, 832, 975, 1067, 1270
BspCNI CTCAG 6 cut(s) 95, 190, 479, 801, 960, 1306
BspHI TCATGA 1 cut(s) 930
BspLI GGNNCC 3 cut(s) 96, 148, 1270
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 1 cut(s) 1341
BspT107I GGYRCC 1 cut(s) 146
BspTI CTTAAG 1 cut(s) 505
BsrGI TGTACA 1 cut(s) 1193
BsrI ACTGG 1 cut(s) 1048
BssECI CCNNGG 2 cut(s) 429, 1287
BssMI GATC 2 cut(s) 805, 1333
BssT1I CCWWGG 2 cut(s) 429, 1287
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 1051
Bst6I CTCTTC 2 cut(s) 1000, 1077
BstAFI CTTAAG 1 cut(s) 505
BstAUI TGTACA 1 cut(s) 1193
BstC8I GCNNGC 1 cut(s) 66
BstENI CCTNNNNNAGG 1 cut(s) 155
BstKTI GATC 2 cut(s) 808, 1336
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 2 cut(s) 805, 1333
BstMWI GCNNNNNNNGC 2 cut(s) 178, 1035
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 1 cut(s) 1385
BstSCI CCNGG 1 cut(s) 211
BstSFI CTRYAG 1 cut(s) 1362
BstSLI GKGCMC 2 cut(s) 151, 1272
BstV1I GCAGC 2 cut(s) 678, 871
BstV2I GAAGAC 2 cut(s) 246, 417
BsuRI GGCC 7 cut(s) 172, 211, 279, 832, 975, 1067, 1270
BtrI CACGTC 1 cut(s) 259
BtsI GCAGTG 1 cut(s) 1011
BtsIMutI CAGTG 2 cut(s) 1011, 1041
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 1 cut(s) 66
CciI TCATGA 1 cut(s) 930
Cfr13I GGNCC 5 cut(s) 278, 830, 974, 1268, 1269
Csp6I GTAC 3 cut(s) 195, 871, 1194
CspCI CAANNNNNGTGG 2 cut(s) 592, 627
CviAII CATG 6 cut(s) 192, 874, 931, 1235, 1374, 1382
CviQI GTAC 3 cut(s) 195, 871, 1194
DpnI GATC 2 cut(s) 807, 1335
DpnII GATC 2 cut(s) 805, 1333
DriI GACNNNNNGTC 1 cut(s) 1053
EaeI YGGCCR 1 cut(s) 1065
Eam1104I CTCTTC 2 cut(s) 1000, 1077
Eam1105I GACNNNNNGTC 1 cut(s) 1053
EarI CTCTTC 2 cut(s) 1000, 1077
Eco130I CCWWGG 2 cut(s) 429, 1287
Eco24I GRGCYC 1 cut(s) 1272
EcoNI CCTNNNNNAGG 1 cut(s) 155
EcoO109I RGGNCCY 1 cut(s) 1268
EcoRI GAATTC 1 cut(s) 1393
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 2 cut(s) 429, 1287
EcoT38I GRGCYC 1 cut(s) 1272
ErhI CCWWGG 2 cut(s) 429, 1287
FaeI CATG 6 cut(s) 195, 877, 934, 1238, 1377, 1385
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 4 cut(s) 248, 313, 533, 1060
FatI CATG 6 cut(s) 191, 873, 930, 1234, 1373, 1381
Fnu4HI GCNGC 2 cut(s) 692, 860
FriOI GRGCYC 1 cut(s) 1272
Fsp4HI GCNGC 2 cut(s) 692, 860
FspBI CTAG 4 cut(s) 101, 176, 440, 1230
GluI GCNGC 2 cut(s) 692, 860
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 7 cut(s) 172, 211, 279, 832, 975, 1067, 1270
Hin1II CATG 6 cut(s) 195, 877, 934, 1238, 1377, 1385
HinfI GANTC 8 cut(s) 80, 161, 202, 326, 811, 934, 1006, 1258
HphI GGTGA 4 cut(s) 283, 482, 1130, 1307
Hpy166II GTNNAC 1 cut(s) 1298
Hpy188I TCNGA 9 cut(s) 85, 166, 325, 516, 558, 672, 810, 958, 1315
Hpy188III TCNNGA 9 cut(s) 155, 176, 680, 770, 898, 931, 1082, 1133, 1337
Hpy8I GTNNAC 1 cut(s) 1298
HpyAV CCTTC 5 cut(s) 109, 161, 341, 677, 1210
HpyCH4III ACNGT 1 cut(s) 1051
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 5 cut(s) 53, 303, 419, 859, 1348
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 1035
HpySE526I ACGT 1 cut(s) 258
Hsp92II CATG 6 cut(s) 195, 877, 934, 1238, 1377, 1385
Kzo9I GATC 2 cut(s) 805, 1333
LmnI GCTCC 1 cut(s) 118
Lsp1109I GCAGC 2 cut(s) 678, 871
MaeI CTAG 4 cut(s) 101, 176, 440, 1230
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 6 cut(s) 259, 391, 522, 596, 812, 1377
MalI GATC 2 cut(s) 807, 1335
MboI GATC 2 cut(s) 805, 1333
MboII GAAGA 8 cut(s) 3, 251, 417, 987, 1015, 1064, 1148, 1362
MhlI GDGCHC 3 cut(s) 151, 183, 1272
MlsI TGGCCA 1 cut(s) 1067
MluCI AATT 7 cut(s) 474, 495, 532, 630, 1060, 1093, 1393
MluNI TGGCCA 1 cut(s) 1067
MlyI GAGTC 5 cut(s) 74, 155, 196, 335, 820
MmeI TCCRAC 2 cut(s) 482, 621
Mox20I TGGCCA 1 cut(s) 1067
MscI TGGCCA 1 cut(s) 1067
MseI TTAA 5 cut(s) 506, 665, 737, 821, 1112
MslI CAYNNNNRTG 1 cut(s) 190
Msp20I TGGCCA 1 cut(s) 1067
MspA1I CMGCKG 1 cut(s) 691
MspCI CTTAAG 1 cut(s) 505
MspR9I CCNGG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 213
MwoI GCNNNNNNNGC 2 cut(s) 178, 1035
NdeII GATC 2 cut(s) 805, 1333
NlaIII CATG 6 cut(s) 195, 877, 934, 1238, 1377, 1385
NlaIV GGNNCC 3 cut(s) 96, 148, 1270
NmuCI GTSAC 5 cut(s) 259, 391, 522, 812, 1377
NspI RCATGY 1 cut(s) 1385
PagI TCATGA 1 cut(s) 930
PciI ACATGT 1 cut(s) 1381
PfeI GAWTC 3 cut(s) 934, 1006, 1258
PflFI GACNNNGTC 1 cut(s) 520
PflMI CCANNNNNTGG 1 cut(s) 1288
PkrI GCNGC 2 cut(s) 693, 861
PleI GAGTC 5 cut(s) 74, 155, 196, 334, 819
PpsI GAGTC 5 cut(s) 74, 155, 196, 334, 819
PscI ACATGT 1 cut(s) 1381
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 3 cut(s) 96, 148, 1270
PspOMI GGGCCC 1 cut(s) 1268
PspPI GGNCC 5 cut(s) 278, 830, 974, 1268, 1269
PsyI GACNNNGTC 1 cut(s) 520
PvuII CAGCTG 1 cut(s) 691
RsaI GTAC 3 cut(s) 196, 872, 1195
RsaNI GTAC 3 cut(s) 195, 871, 1194
RseI CAYNNNNRTG 1 cut(s) 190
SaqAI TTAA 5 cut(s) 506, 665, 737, 821, 1112
SatI GCNGC 2 cut(s) 692, 860
Sau3AI GATC 2 cut(s) 805, 1333
Sau96I GGNCC 5 cut(s) 278, 830, 974, 1268, 1269
SchI GAGTC 5 cut(s) 74, 155, 196, 335, 820
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 3 cut(s) 151, 183, 1272
SfcI CTRYAG 1 cut(s) 1362
SmiMI CAYNNNNRTG 1 cut(s) 190
SmlI CTYRAG 5 cut(s) 155, 481, 505, 592, 607
SmoI CTYRAG 5 cut(s) 155, 481, 505, 592, 607
Sse9I AATT 7 cut(s) 474, 495, 532, 630, 1060, 1093, 1393
SspI AATATT 2 cut(s) 366, 1225
SspMI CTAG 4 cut(s) 101, 176, 440, 1230
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 2 cut(s) 429, 1287
TaaI ACNGT 1 cut(s) 1051
TaiI ACGT 1 cut(s) 261
TasI AATT 7 cut(s) 474, 495, 532, 630, 1060, 1093, 1393
TatI WGTACW 3 cut(s) 194, 870, 1193
TfiI GAWTC 3 cut(s) 934, 1006, 1258
Tru1I TTAA 5 cut(s) 506, 665, 737, 821, 1112
Tru9I TTAA 5 cut(s) 506, 665, 737, 821, 1112
TscAI CASTG 2 cut(s) 1018, 1048
TseFI GTSAC 5 cut(s) 259, 391, 522, 812, 1377
TseI GCWGC 2 cut(s) 691, 859
Tsp45I GTSAC 5 cut(s) 259, 391, 522, 812, 1377
TspDTI ATGAA 5 cut(s) 947, 1132, 1347, 1362, 1386
TspRI CASTG 2 cut(s) 1018, 1048
Tth111I GACNNNGTC 1 cut(s) 520
Van91I CCANNNNNTGG 1 cut(s) 1288
Vha464I CTTAAG 1 cut(s) 505
XagI CCTNNNNNAGG 1 cut(s) 155
XapI RAATTY 5 cut(s) 474, 495, 1060, 1093, 1393
XbaI TCTAGA 1 cut(s) 175
XceI RCATGY 1 cut(s) 1385
XcmI CCANNNNNNNNNTGG 1 cut(s) 1289
XspI CTAG 4 cut(s) 101, 176, 440, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.