MD05G1281800.v1.1

Leucine Rich Repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016779
Physical Location & Seq
Forward (+)
41568324 .. 41569751
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1281800.v1.1.491

Sequence Viewer

Length: 1428 bp
ATGGCTTCTTTGCCTCTTTTCCTTGTTGTGTTTCTTGTTTTGGGAGGGGGGTTGATTTCAGGGCAGCAAAACTCAGATGGGATTATGGAAAAGGAGGAGCTTTTGGGACTTTTTGAAGTTTTGGGTGGCCTTCTGGAGGACCCCAGCTGGGCCCAAGAGCACCCACAGCCCTGTGATGAGACTCCATGGCCTGGTATTCAATGTGAAATTGGTGATGAAAATCCTCCAAGTTTTCATGTCACAAAGATTCACATTGGCCCTGATTTTCTCAGCCCTCCTTGCAAATCCTCTGCTTCCCTTTCAAGTTCCCTCCTCAAATTACCCTACTTGAAAACCCTCTCAATTTTCAATTGCTTTTTAACTACACCGGTCACTCTCTCTCCATCACTATTTGGTGCATTGTCTTCCTTGGAAACCCTAGCCCTTGTTTCCAATCCAGCTCTCTCAGGAGAAATCCCCCCAGAATTGTCAAAAATTACAAACTTAAGGGTCCTCAGCCTCTCACAGAACAACCTACTAGGAAACATCCCTAGTGAAATTGGTGGGCTGGTGAGACTAGAGCAGCTTGACCTAAGCTACAACAACCTAAGTGGTGAACTCCCACAGGAAATTGGAGGGTTGGGGAGTTTGACAATCTTGGATTTGAGCTGGAATGTTCTAGAAGGTCAGGTGCCTAGCTCTGTAGGGCAGCTTCAAGTCCTCCAAAAGATTGATATGAGCTGCAACATGCTCAGAGGATTGATCCCTCCAAATGTAGGGCAGCTCAAGAGGTTGGTGTTGTTTGATCTGAGTCATAACTTGATCAATGGGCCAATCCCAGAAACCCTTTCAGGTTTGGAGAATTTAGAGTATTGGGTAGCTGATAAAAACCCAATCAATTCAGAAATTCCTCAGTTTGTAGGGAAACTTAAGAATCTCATATCTCTGAGCTTTTCAGGATGTGGGTTGATTGGCAAATTACCCAACTCCCTATCTTCCTTGAAAAATCTCAGTTCCCTCTCTCTAGACAACAACAGTCTCAATGGGACAGTCCCTACAAGTTTAGGGACACTCCCAAAATTGGATCAGCTGAATCTGAGCAACAACCAGCTGAGTGGGGCCCTGTCACTTCCAGAGGATTTCATTGGGAGGCTTGGGAAGAGGTTGGATGTGAGAGGAAATAATGGTCTGTGTGCAATCAATCCATTGTACAATAAGAAGAAGGGCATCTCTGCAAATCTGATAAGTGCCCCTGTTTGTTTGAATGCAAGTGGAGCAACAAATGACACGGCCTTGGCCGGTGAAGACAAAGAACCAAAAGTGTCCGAGAGAATGAAGCCATTTGGTCAGTACCCTGTTGAGAACAATAGTTCCGGTTCTCCATCGAATTCAAATGCAAAGATGGTTTCTTTTGGTTTTGTTGTGTGTTTCTTGTGCCTCTTGTTGTAA

Protein Analysis

476

Amino Acids

50.69

Weight (kDa)

4.93

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 156 - 239 2.7e-10 Leucine-rich repeat region
LRR_8 PF13855 160 - 220 1.6e-08 Leucine rich repeat
LRR_14 PF23598 294 - 362 1e-05 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016779)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05310
malus_domestica MD05G1281800.v1.1
prunus_persica Prupe.4G082700_v2.0.a1
pyrus_communis pycom05g26170
rosa_chinensis RchiOBHm_Chr5g0014421
rosa_laevigata RLG00000032107
rosa_multiflora Rmu_ssc0000422.1_g000037
rosa_roxburghii Rroxscaffold_1G00062160
rosa_rugosa Rorug05G0017900
rosa_samantha Rh5AG112800 Rh5BG109600 Rh5CG121400 Rh5DG108600
rosa_wichuraiana Rw5G009790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 670
AccB7I CCANNNNNTGG 1 cut(s) 191
AclWI GGATC 2 cut(s) 736, 1071
AcoI YGGCCR 1 cut(s) 1275
AcsI RAATTY 3 cut(s) 841, 885, 1366
AfaI GTAC 2 cut(s) 1190, 1331
AfiI CCNNNNNNNGG 5 cut(s) 136, 148, 191, 755, 1060
AflII CTTAAG 2 cut(s) 484, 908
AgeI ACCGGT 1 cut(s) 367
AgsI TTSAA 9 cut(s) 116, 200, 303, 331, 349, 695, 982, 1243, 1371
AjnI CCWGG 1 cut(s) 190
AloI GAACNNNNNNTCC 2 cut(s) 1334, 1366
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 3 cut(s) 173, 547, 1022
AlwI GGATC 2 cut(s) 736, 1071
AoxI GGCC 8 cut(s) 127, 150, 188, 256, 809, 1098, 1269, 1275
ApaI GGGCCC 2 cut(s) 154, 1102
ApeKI GCWGC 5 cut(s) 64, 562, 688, 720, 760
ApoI RAATTY 3 cut(s) 841, 885, 1366
AsiGI ACCGGT 1 cut(s) 367
AspS9I GGNCC 8 cut(s) 139, 150, 151, 257, 490, 809, 1098, 1099
AsuHPI GGTGA 4 cut(s) 224, 562, 605, 1292
AvaII GGWCC 2 cut(s) 139, 490
BaeGI GKGCMC 3 cut(s) 154, 1102, 1231
BanI GGYRCC 1 cut(s) 670
BanII GRGCYC 2 cut(s) 154, 1102
BarI GAAGNNNNNNTAC 2 cut(s) 675, 707
BbsI GAAGAC 2 cut(s) 396, 1290
Bbv12I GWGCWC 1 cut(s) 162
BbvCI CCTCAGC 1 cut(s) 494
BbvI GCAGC 5 cut(s) 76, 574, 700, 707, 772
BccI CCATC 4 cut(s) 71, 391, 1369, 1375
BceAI ACGGC 1 cut(s) 1284
BciT130I CCWGG 1 cut(s) 192
BclI TGATCA 1 cut(s) 801
BcoDI GTCTC 3 cut(s) 173, 547, 1022
BfaI CTAG 7 cut(s) 419, 518, 531, 557, 659, 675, 1004
BfmI CTRYAG 1 cut(s) 681
BfrI CTTAAG 2 cut(s) 484, 908
BisI GCNGC 5 cut(s) 65, 563, 689, 721, 761
BlsI GCNGC 5 cut(s) 66, 564, 690, 722, 762
Bme1390I CCNGG 1 cut(s) 192
Bme18I GGWCC 2 cut(s) 139, 490
BmgT120I GGNCC 8 cut(s) 139, 150, 151, 257, 490, 809, 1098, 1099
BmiI GGNNCC 6 cut(s) 141, 152, 491, 672, 1099, 1100
BmrFI CCNGG 1 cut(s) 192
BmsI GCATC 1 cut(s) 1215
BpiI GAAGAC 2 cut(s) 396, 1290
BpmI CTGGAG 1 cut(s) 155
Bpu10I CCTNAGC 2 cut(s) 494, 572
BpuEI CTTGAG 1 cut(s) 749
BsaBI GATNNNNATC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 185, 408, 1272
BsaWI WCCGGW 2 cut(s) 367, 1352
BsaXI ACNNNNNCTCC 2 cut(s) 364, 394
Bsc4I CCNNNNNNNGG 5 cut(s) 136, 148, 191, 755, 1060
Bse118I RCCGGY 2 cut(s) 367, 1277
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 1 cut(s) 192
BseDI CCNNGG 3 cut(s) 185, 408, 1272
BseGI GGATG 3 cut(s) 525, 944, 1153
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 5 cut(s) 136, 148, 191, 755, 1060
BseRI GAGGAG 2 cut(s) 110, 302
BseSI GKGCMC 3 cut(s) 154, 1102, 1231
BseXI GCAGC 5 cut(s) 76, 574, 700, 707, 772
BseYI CCCAGC 2 cut(s) 143, 147
BshFI GGCC 8 cut(s) 129, 152, 190, 258, 811, 1100, 1271, 1277
BshNI GGYRCC 1 cut(s) 670
BshTI ACCGGT 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 3 cut(s) 368, 1278, 1353
BslFI GGGAC 4 cut(s) 120, 1016, 1039, 1060
BslI CCNNNNNNNGG 5 cut(s) 136, 148, 191, 755, 1060
BsmAI GTCTC 3 cut(s) 173, 547, 1022
BsmFI GGGAC 4 cut(s) 120, 1016, 1039, 1060
BsmI GAATGC 1 cut(s) 1249
BsnI GGCC 8 cut(s) 129, 152, 190, 258, 811, 1100, 1271, 1277
Bsp120I GGGCCC 2 cut(s) 150, 1098
Bsp1286I GDGCHC 4 cut(s) 154, 162, 1102, 1231
Bsp1407I TGTACA 1 cut(s) 1188
Bsp143I GATC 4 cut(s) 741, 784, 801, 1063
Bsp19I CCATGG 1 cut(s) 185
BspANI GGCC 8 cut(s) 129, 152, 190, 258, 811, 1100, 1271, 1277
BspLI GGNNCC 6 cut(s) 141, 152, 491, 672, 1099, 1100
BspPI GGATC 2 cut(s) 736, 1071
BspT107I GGYRCC 1 cut(s) 670
BspTI CTTAAG 2 cut(s) 484, 908
BsrFI RCCGGY 2 cut(s) 367, 1277
BsrGI TGTACA 1 cut(s) 1188
BssAI RCCGGY 2 cut(s) 367, 1277
BssECI CCNNGG 3 cut(s) 185, 408, 1272
BssMI GATC 4 cut(s) 741, 784, 801, 1063
BssT1I CCWWGG 3 cut(s) 185, 408, 1272
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 1016, 1030
Bst6I CTCTTC 1 cut(s) 1133
BstAFI CTTAAG 2 cut(s) 484, 908
BstAUI TGTACA 1 cut(s) 1188
BstDSI CCRYGG 1 cut(s) 185
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 3 cut(s) 525, 944, 1153
BstKTI GATC 4 cut(s) 744, 787, 804, 1066
BstMAI GTCTC 3 cut(s) 173, 547, 1022
BstMBI GATC 4 cut(s) 741, 784, 801, 1063
BstMWI GCNNNNNNNGC 3 cut(s) 166, 279, 1253
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 1 cut(s) 190
BstSFI CTRYAG 1 cut(s) 681
BstSLI GKGCMC 3 cut(s) 154, 1102, 1231
BstV1I GCAGC 5 cut(s) 76, 574, 700, 707, 772
BstV2I GAAGAC 2 cut(s) 396, 1290
BstXI CCANNNNNNTGG 1 cut(s) 1094
BsuRI GGCC 8 cut(s) 129, 152, 190, 258, 811, 1100, 1271, 1277
BtgI CCRYGG 1 cut(s) 185
BtsCI GGATG 3 cut(s) 525, 944, 1153
Cfr10I RCCGGY 2 cut(s) 367, 1277
Cfr13I GGNCC 8 cut(s) 139, 150, 151, 257, 490, 809, 1098, 1099
Csp6I GTAC 2 cut(s) 1189, 1330
CspAI ACCGGT 1 cut(s) 367
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 3 cut(s) 186, 236, 727
CviQI GTAC 2 cut(s) 1189, 1330
DpnI GATC 4 cut(s) 743, 786, 803, 1065
DpnII GATC 4 cut(s) 741, 784, 801, 1063
EaeI YGGCCR 1 cut(s) 1275
Eam1104I CTCTTC 1 cut(s) 1133
EarI CTCTTC 1 cut(s) 1133
Eco130I CCWWGG 3 cut(s) 185, 408, 1272
Eco24I GRGCYC 2 cut(s) 154, 1102
Eco47I GGWCC 2 cut(s) 139, 490
EcoNI CCTNNNNNAGG 1 cut(s) 134
EcoO109I RGGNCCY 4 cut(s) 139, 490, 1098, 1099
EcoRI GAATTC 1 cut(s) 1366
EcoRII CCWGG 1 cut(s) 190
EcoT14I CCWWGG 3 cut(s) 185, 408, 1272
EcoT38I GRGCYC 2 cut(s) 154, 1102
ErhI CCWWGG 3 cut(s) 185, 408, 1272
FaeI CATG 3 cut(s) 189, 239, 730
FaiI YATR 7 cut(s) 86, 187, 237, 716, 728, 795, 920
FaqI GGGAC 4 cut(s) 120, 1016, 1039, 1060
FatI CATG 3 cut(s) 185, 235, 726
FbaI TGATCA 1 cut(s) 801
Fnu4HI GCNGC 5 cut(s) 65, 563, 689, 721, 761
FokI GGATG 3 cut(s) 512, 951, 1160
FriOI GRGCYC 2 cut(s) 154, 1102
Fsp4HI GCNGC 5 cut(s) 65, 563, 689, 721, 761
FspBI CTAG 7 cut(s) 419, 518, 531, 557, 659, 675, 1004
GluI GCNGC 5 cut(s) 65, 563, 689, 721, 761
GsaI CCCAGC 2 cut(s) 147, 151
GsuI CTGGAG 1 cut(s) 155
HaeIII GGCC 8 cut(s) 129, 152, 190, 258, 811, 1100, 1271, 1277
HapII CCGG 3 cut(s) 368, 1278, 1353
Hin1II CATG 3 cut(s) 189, 239, 730
HinfI GANTC 5 cut(s) 181, 247, 790, 913, 1072
HpaII CCGG 3 cut(s) 368, 1278, 1353
HphI GGTGA 4 cut(s) 224, 562, 605, 1292
Hpy166II GTNNAC 1 cut(s) 596
Hpy188I TCNGA 8 cut(s) 76, 734, 789, 883, 927, 1077, 1221, 1306
Hpy188III TCNNGA 7 cut(s) 134, 447, 659, 766, 936, 1004, 1112
Hpy8I GTNNAC 1 cut(s) 596
HpyAV CCTTC 3 cut(s) 140, 656, 1195
HpyCH4III ACNGT 2 cut(s) 1016, 1030
HpyCH4V TGCA 7 cut(s) 282, 398, 723, 1175, 1214, 1247, 1376
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 279, 1253
Hsp92II CATG 3 cut(s) 189, 239, 730
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 4 cut(s) 741, 784, 801, 1063
LmnI GCTCC 2 cut(s) 97, 1253
Lsp1109I GCAGC 5 cut(s) 76, 574, 700, 707, 772
LweI GCATC 1 cut(s) 1215
MaeI CTAG 7 cut(s) 419, 518, 531, 557, 659, 675, 1004
MaeIII GTNAC 3 cut(s) 238, 370, 1104
MalI GATC 4 cut(s) 743, 786, 803, 1065
MboI GATC 4 cut(s) 741, 784, 801, 1063
MboII GAAGA 5 cut(s) 396, 966, 1150, 1210, 1295
MfeI CAATTG 1 cut(s) 349
MhlI GDGCHC 4 cut(s) 154, 162, 1102, 1231
MlyI GAGTC 2 cut(s) 175, 799
MmeI TCCRAC 1 cut(s) 1125
MseI TTAA 3 cut(s) 359, 485, 909
MspA1I CMGCKG 3 cut(s) 147, 1069, 1090
MspCI CTTAAG 2 cut(s) 484, 908
MspI CCGG 3 cut(s) 368, 1278, 1353
MspR9I CCNGG 1 cut(s) 192
MunI CAATTG 1 cut(s) 349
Mva1269I GAATGC 1 cut(s) 1249
MvaI CCWGG 1 cut(s) 192
MwoI GCNNNNNNNGC 3 cut(s) 166, 279, 1253
NcoI CCATGG 1 cut(s) 185
NdeII GATC 4 cut(s) 741, 784, 801, 1063
NlaIII CATG 3 cut(s) 189, 239, 730
NlaIV GGNNCC 6 cut(s) 141, 152, 491, 672, 1099, 1100
NmuCI GTSAC 3 cut(s) 238, 370, 1104
NspI RCATGY 1 cut(s) 730
PctI GAATGC 1 cut(s) 1249
PfeI GAWTC 3 cut(s) 247, 913, 1072
PflMI CCANNNNNTGG 1 cut(s) 191
PinAI ACCGGT 1 cut(s) 367
PkrI GCNGC 5 cut(s) 66, 564, 690, 722, 762
PleI GAGTC 2 cut(s) 175, 798
PpsI GAGTC 2 cut(s) 175, 798
PpuMI RGGWCCY 2 cut(s) 139, 490
Psp5II RGGWCCY 2 cut(s) 139, 490
Psp6I CCWGG 1 cut(s) 190
PspFI CCCAGC 2 cut(s) 143, 147
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 6 cut(s) 141, 152, 491, 672, 1099, 1100
PspOMI GGGCCC 2 cut(s) 150, 1098
PspPI GGNCC 8 cut(s) 139, 150, 151, 257, 490, 809, 1098, 1099
PspPPI RGGWCCY 2 cut(s) 139, 490
PvuII CAGCTG 3 cut(s) 147, 1069, 1090
RsaI GTAC 2 cut(s) 1190, 1331
RsaNI GTAC 2 cut(s) 1189, 1330
SaqAI TTAA 3 cut(s) 359, 485, 909
SatI GCNGC 5 cut(s) 65, 563, 689, 721, 761
Sau3AI GATC 4 cut(s) 741, 784, 801, 1063
Sau96I GGNCC 8 cut(s) 139, 150, 151, 257, 490, 809, 1098, 1099
SchI GAGTC 2 cut(s) 175, 799
ScrFI CCNGG 1 cut(s) 192
SduI GDGCHC 4 cut(s) 154, 162, 1102, 1231
SfaNI GCATC 1 cut(s) 1215
SfcI CTRYAG 1 cut(s) 681
SinI GGWCC 2 cut(s) 139, 490
SmlI CTYRAG 3 cut(s) 484, 764, 908
SmoI CTYRAG 3 cut(s) 484, 764, 908
SspMI CTAG 7 cut(s) 419, 518, 531, 557, 659, 675, 1004
StyD4I CCNGG 1 cut(s) 190
StyI CCWWGG 3 cut(s) 185, 408, 1272
TaaI ACNGT 2 cut(s) 1016, 1030
TaqI TCGA 1 cut(s) 1364
TatI WGTACW 1 cut(s) 1188
TfiI GAWTC 3 cut(s) 247, 913, 1072
Tru1I TTAA 3 cut(s) 359, 485, 909
Tru9I TTAA 3 cut(s) 359, 485, 909
TseFI GTSAC 3 cut(s) 238, 370, 1104
TseI GCWGC 5 cut(s) 64, 562, 688, 720, 760
Tsp45I GTSAC 3 cut(s) 238, 370, 1104
TspDTI ATGAA 4 cut(s) 224, 231, 1111, 1328
Van91I CCANNNNNTGG 1 cut(s) 191
Vha464I CTTAAG 2 cut(s) 484, 908
VpaK11BI GGWCC 2 cut(s) 139, 490
XagI CCTNNNNNAGG 1 cut(s) 134
XapI RAATTY 3 cut(s) 841, 885, 1366
XbaI TCTAGA 2 cut(s) 658, 1003
XceI RCATGY 1 cut(s) 730
XspI CTAG 7 cut(s) 419, 518, 531, 557, 659, 675, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.