RLG00000032107

Leucine Rich Repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016779
Physical Location & Seq
Reverse (-)
11030903 .. 11032675
1773 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032107

Sequence Viewer

Length: 1482 bp
ATGGCTTTTTCTTCTTCTGTGTGTCTTTTCCTTGTTGGGTTTCTTGTTTTTGCAGGGAAGTTGCTTGCTGAAATGCCTGACTCAGATGATGAGGTTCCACTAGAGATGGAAAAGAAGGAGCTTTTGGGGATTTTTGAGGTAATGGGTGCCCTTCTTGAGGACTCTGATTGGCCTCTAGAGCACCCACAACCATGTACTGAGACTCAATGGCCTGGTGTTCAATGTGAGATTGGGGACGAGGACCCTCCAATGTTTCATGTCACAAAGATACACATTGGCCCTGATATTCTCACCCCTCCTTGCAAATCATCTGCTACTTTATCAGAGTCCCTTCTCAAACTACCTTACTTGAAAACCCTCTCAATATTTAGTTGCTTTTCAACATCACAAGTCACTCTTTCTCCAAAGCTCTTTGGTGCATTGTCTTCCTTGGAACACCTAGCCCTTGTGTCCAATCCAACTCTCTCAGGTGAAATTCCCTCAAGTTTAGCCCAAATTTCCAGCTTAAGGGTTCTGACTTTGTCACAGAACAATTTACAAGGAAAAGTCCCTAGTAAGATTGGTGCTTTGGTCAGCTTAGAGCAACTTGACTTGAGTTACAACAACTTGAGTGGTCAAATACCAGAAGAAATTGGAGGGTTGGAGAGTTTGGCAATTTTGGATTTAAGTCAGAATGTTCTTGAAGGTCAGTTGCCCTCCTCTTTTGGTCAGCTTCAACTCCTCCAAAAGATTGATTTAAGCTCAAACAGGCTTGGAGGTATATTGCCTCCAGATATGGGGAAGCTCAACAGGTTAGTTTTGCTTGATCTGAGTCACAACTTAATAAATGGGCCAATCCCTGAAACCCTTTCAGGTTTACAGCATTTAGAGTACATGGTAGCTGATAGCAACCCAATCAAGACAGAAGTTCCTAACTTTGTTGGGAAGCTCATGAATCTCAAAACACTCAGCTTTTCAGAATGTGGGTTGATAGGCCCATTACCCAACTTTCTCTCTTCCTTGAAGAATCTCACTGCTCTTTCTCTAAGCAACAACAGCCTCACTGGGACTGTTCGTCCAAATTTGGGCACTCTTCCAAGTCTTGATTTACTAAATTTGAGCAACAATCAGTTAAGTGGTGAGCTGACACTTCCAGAAGATTTCATTGAGAGGCTTGGGAAGAGGTTGGTGCTAAGAGGAAACAATGGGCTATGTACAGGCAATCCACTCAACAAGAAGGAGAACATTTCTAGCCATGTTATAGCCCCTCTGTGTTTGAATCCAAGAGGGCCCAGAGATGACCACACCTTGGCTGGTGAACACCCAACTGAGTCTGAGAGAGTGAAGCCATTGCGTCAAAACCCTGTGCAGGAAAGTTCATCTACAGGTATTGAACTTGATTTCTTGCTAAATGTCAACACCTTCAGTTCATGTTCTTTATTTTCTGTGGTCCTTTTTCTAACAGAAGAATTTCAGTCTTTCAGTTTCAGATTTGGCTTTTAA

Protein Analysis

494

Amino Acids

53.58

Weight (kDa)

4.84

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 161 - 246 1.6e-10 Leucine-rich repeat region
LRR_8 PF13855 162 - 203 5.8e-06 Leucine rich repeat
LRR_8 PF13855 192 - 251 1.2e-07 Leucine rich repeat
LRR_8 PF13855 241 - 299 2.2e-07 Leucine rich repeat
LRR_14 PF23598 255 - 340 2.9e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016779)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05310
malus_domestica MD05G1281800.v1.1
prunus_persica Prupe.4G082700_v2.0.a1
pyrus_communis pycom05g26170
rosa_chinensis RchiOBHm_Chr5g0014421
rosa_laevigata RLG00000032107
rosa_multiflora Rmu_ssc0000422.1_g000037
rosa_roxburghii Rroxscaffold_1G00062160
rosa_rugosa Rorug05G0017900
rosa_samantha Rh5AG112800 Rh5BG109600 Rh5CG121400 Rh5DG108600
rosa_wichuraiana Rw5G009790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 1288
AcsI RAATTY 5 cut(s) 474, 495, 1060, 1093, 1448
AcuI CTGAAG 1 cut(s) 1387
AfaI GTAC 3 cut(s) 196, 872, 1195
AfiI CCNNNNNNNGG 6 cut(s) 157, 507, 776, 1064, 1288, 1348
AflII CTTAAG 1 cut(s) 505
AgsI TTSAA 8 cut(s) 221, 352, 381, 683, 716, 1003, 1258, 1373
AhdI GACNNNNNGTC 1 cut(s) 1053
AjnI CCWGG 1 cut(s) 211
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 1 cut(s) 194
AoxI GGCC 6 cut(s) 170, 209, 277, 830, 973, 1268
ApaI GGGCCC 1 cut(s) 1272
ApoI RAATTY 5 cut(s) 474, 495, 1060, 1093, 1448
Asp700I GAANNNNTTC 1 cut(s) 1449
AspS9I GGNCC 7 cut(s) 241, 278, 830, 974, 1268, 1269, 1429
AsuHPI GGTGA 4 cut(s) 283, 482, 1130, 1307
AvaII GGWCC 2 cut(s) 241, 1429
BaeGI GKGCMC 3 cut(s) 151, 1070, 1272
BanI GGYRCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 1272
BbsI GAAGAC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 183
BccI CCATC 1 cut(s) 100
BciT130I CCWGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 5 cut(s) 101, 176, 440, 552, 1230
BfmI CTRYAG 1 cut(s) 1362
BfrI CTTAAG 1 cut(s) 505
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 2 cut(s) 241, 1429
BmeRI GACNNNNNGTC 1 cut(s) 1053
BmgT120I GGNCC 7 cut(s) 241, 278, 830, 974, 1268, 1269, 1429
BmiI GGNNCC 4 cut(s) 96, 148, 243, 1270
BmrFI CCNGG 1 cut(s) 213
BmrI ACTGGG 1 cut(s) 1053
BmuI ACTGGG 1 cut(s) 1053
BpiI GAAGAC 1 cut(s) 417
BpmI CTGGAG 1 cut(s) 753
BpuEI CTTGAG 4 cut(s) 176, 466, 613, 628
BsaJI CCNNGG 2 cut(s) 429, 1287
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
Bsc4I CCNNNNNNNGG 6 cut(s) 157, 507, 776, 1064, 1288, 1348
Bse1I ACTGG 1 cut(s) 1048
Bse3DI GCAATG 1 cut(s) 1328
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 429, 1287
BseLI CCNNNNNNNGG 6 cut(s) 157, 507, 776, 1064, 1288, 1348
BseMI GCAATG 1 cut(s) 1328
BseMII CTCAG 7 cut(s) 96, 189, 480, 800, 961, 1299, 1305
BseNI ACTGG 1 cut(s) 1048
BseRI GAGGAG 2 cut(s) 688, 710
BseSI GKGCMC 3 cut(s) 151, 1070, 1272
BsgI GTGCAG 1 cut(s) 1367
BshFI GGCC 6 cut(s) 172, 211, 279, 832, 975, 1270
BshNI GGYRCC 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 183
BslFI GGGAC 4 cut(s) 248, 313, 533, 1060
BslI CCNNNNNNNGG 6 cut(s) 157, 507, 776, 1064, 1288, 1348
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 4 cut(s) 248, 313, 533, 1060
BsnI GGCC 6 cut(s) 172, 211, 279, 832, 975, 1270
Bsp120I GGGCCC 1 cut(s) 1268
Bsp1286I GDGCHC 4 cut(s) 151, 183, 1070, 1272
Bsp1407I TGTACA 1 cut(s) 1193
Bsp143I GATC 1 cut(s) 805
BspANI GGCC 6 cut(s) 172, 211, 279, 832, 975, 1270
BspCNI CTCAG 7 cut(s) 95, 190, 479, 801, 960, 1300, 1306
BspHI TCATGA 1 cut(s) 930
BspLI GGNNCC 4 cut(s) 96, 148, 243, 1270
BspT107I GGYRCC 1 cut(s) 146
BspTI CTTAAG 1 cut(s) 505
BsrDI GCAATG 1 cut(s) 1328
BsrGI TGTACA 1 cut(s) 1193
BsrI ACTGG 1 cut(s) 1048
BssECI CCNNGG 2 cut(s) 429, 1287
BssMI GATC 1 cut(s) 805
BssT1I CCWWGG 2 cut(s) 429, 1287
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 1051
Bst6I CTCTTC 3 cut(s) 1000, 1077, 1154
BstAFI CTTAAG 1 cut(s) 505
BstAUI TGTACA 1 cut(s) 1193
BstC8I GCNNGC 1 cut(s) 66
BstENI CCTNNNNNAGG 1 cut(s) 155
BstKTI GATC 1 cut(s) 808
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 1 cut(s) 805
BstMWI GCNNNNNNNGC 2 cut(s) 178, 1035
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 211
BstSFI CTRYAG 1 cut(s) 1362
BstSLI GKGCMC 3 cut(s) 151, 1070, 1272
BstV2I GAAGAC 1 cut(s) 417
BsuRI GGCC 6 cut(s) 172, 211, 279, 832, 975, 1270
BtsI GCAGTG 1 cut(s) 1011
BtsIMutI CAGTG 2 cut(s) 1011, 1041
Cac8I GCNNGC 1 cut(s) 66
CciI TCATGA 1 cut(s) 930
Cfr13I GGNCC 7 cut(s) 241, 278, 830, 974, 1268, 1269, 1429
CseI GACGC 1 cut(s) 1322
Csp6I GTAC 3 cut(s) 195, 871, 1194
CspCI CAANNNNNGTGG 2 cut(s) 592, 627
CviAII CATG 6 cut(s) 192, 257, 874, 931, 1235, 1410
CviQI GTAC 3 cut(s) 195, 871, 1194
DpnI GATC 1 cut(s) 807
DpnII GATC 1 cut(s) 805
DriI GACNNNNNGTC 1 cut(s) 1053
Eam1104I CTCTTC 3 cut(s) 1000, 1077, 1154
Eam1105I GACNNNNNGTC 1 cut(s) 1053
EarI CTCTTC 3 cut(s) 1000, 1077, 1154
Eco130I CCWWGG 2 cut(s) 429, 1287
Eco24I GRGCYC 1 cut(s) 1272
Eco47I GGWCC 2 cut(s) 241, 1429
Eco57I CTGAAG 1 cut(s) 1387
EcoNI CCTNNNNNAGG 1 cut(s) 155
EcoO109I RGGNCCY 2 cut(s) 241, 1268
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 2 cut(s) 429, 1287
EcoT38I GRGCYC 1 cut(s) 1272
ErhI CCWWGG 2 cut(s) 429, 1287
FaeI CATG 6 cut(s) 195, 260, 877, 934, 1238, 1413
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 4 cut(s) 248, 313, 533, 1060
FatI CATG 6 cut(s) 191, 256, 873, 930, 1234, 1409
FriOI GRGCYC 1 cut(s) 1272
FspBI CTAG 5 cut(s) 101, 176, 440, 552, 1230
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 6 cut(s) 172, 211, 279, 832, 975, 1270
HgaI GACGC 1 cut(s) 1322
Hin1II CATG 6 cut(s) 195, 260, 877, 934, 1238, 1413
HincII GTYRAC 1 cut(s) 1396
HindII GTYRAC 1 cut(s) 1396
HinfI GANTC 9 cut(s) 80, 161, 202, 326, 811, 934, 1006, 1258, 1310
HphI GGTGA 4 cut(s) 283, 482, 1130, 1307
Hpy166II GTNNAC 3 cut(s) 857, 1298, 1396
Hpy188I TCNGA 9 cut(s) 85, 166, 325, 516, 672, 810, 958, 1315, 1469
Hpy188III TCNNGA 8 cut(s) 155, 176, 680, 770, 898, 931, 1082, 1133
Hpy8I GTNNAC 3 cut(s) 857, 1298, 1396
HpyAV CCTTC 6 cut(s) 109, 161, 341, 677, 1210, 1411
HpyCH4III ACNGT 1 cut(s) 1051
HpyCH4V TGCA 4 cut(s) 53, 303, 419, 1348
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 1035
Hsp92II CATG 6 cut(s) 195, 260, 877, 934, 1238, 1413
Kzo9I GATC 1 cut(s) 805
LmnI GCTCC 1 cut(s) 118
MaeI CTAG 5 cut(s) 101, 176, 440, 552, 1230
MaeIII GTNAC 5 cut(s) 259, 391, 522, 596, 812
MalI GATC 1 cut(s) 807
MboI GATC 1 cut(s) 805
MhlI GDGCHC 4 cut(s) 151, 183, 1070, 1272
MluCI AATT 8 cut(s) 474, 495, 532, 630, 654, 1060, 1093, 1448
MlyI GAGTC 6 cut(s) 74, 155, 196, 335, 820, 1319
MmeI TCCRAC 2 cut(s) 482, 621
MroXI GAANNNNTTC 1 cut(s) 1449
MseI TTAA 6 cut(s) 506, 665, 737, 821, 1112, 1480
MslI CAYNNNNRTG 1 cut(s) 190
MspCI CTTAAG 1 cut(s) 505
MspR9I CCNGG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 213
MwoI GCNNNNNNNGC 2 cut(s) 178, 1035
NdeII GATC 1 cut(s) 805
NlaIII CATG 6 cut(s) 195, 260, 877, 934, 1238, 1413
NlaIV GGNNCC 4 cut(s) 96, 148, 243, 1270
NmuCI GTSAC 4 cut(s) 259, 391, 522, 812
PagI TCATGA 1 cut(s) 930
PdmI GAANNNNTTC 1 cut(s) 1449
PfeI GAWTC 3 cut(s) 934, 1006, 1258
PflFI GACNNNGTC 1 cut(s) 520
PflMI CCANNNNNTGG 1 cut(s) 1288
PleI GAGTC 6 cut(s) 74, 155, 196, 334, 819, 1318
PpsI GAGTC 6 cut(s) 74, 155, 196, 334, 819, 1318
PpuMI RGGWCCY 1 cut(s) 241
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 4 cut(s) 96, 148, 243, 1270
PspOMI GGGCCC 1 cut(s) 1268
PspPI GGNCC 7 cut(s) 241, 278, 830, 974, 1268, 1269, 1429
PspPPI RGGWCCY 1 cut(s) 241
PsyI GACNNNGTC 1 cut(s) 520
RsaI GTAC 3 cut(s) 196, 872, 1195
RsaNI GTAC 3 cut(s) 195, 871, 1194
RseI CAYNNNNRTG 1 cut(s) 190
SaqAI TTAA 6 cut(s) 506, 665, 737, 821, 1112, 1480
Sau3AI GATC 1 cut(s) 805
Sau96I GGNCC 7 cut(s) 241, 278, 830, 974, 1268, 1269, 1429
SchI GAGTC 6 cut(s) 74, 155, 196, 335, 820, 1319
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 4 cut(s) 151, 183, 1070, 1272
SfcI CTRYAG 1 cut(s) 1362
SinI GGWCC 2 cut(s) 241, 1429
SmiMI CAYNNNNRTG 1 cut(s) 190
SmlI CTYRAG 5 cut(s) 155, 481, 505, 592, 607
SmoI CTYRAG 5 cut(s) 155, 481, 505, 592, 607
Sse9I AATT 8 cut(s) 474, 495, 532, 630, 654, 1060, 1093, 1448
SspI AATATT 1 cut(s) 366
SspMI CTAG 5 cut(s) 101, 176, 440, 552, 1230
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 2 cut(s) 429, 1287
TaaI ACNGT 1 cut(s) 1051
TasI AATT 8 cut(s) 474, 495, 532, 630, 654, 1060, 1093, 1448
TatI WGTACW 3 cut(s) 194, 870, 1193
TfiI GAWTC 3 cut(s) 934, 1006, 1258
Tru1I TTAA 6 cut(s) 506, 665, 737, 821, 1112, 1480
Tru9I TTAA 6 cut(s) 506, 665, 737, 821, 1112, 1480
TscAI CASTG 2 cut(s) 1018, 1048
TseFI GTSAC 4 cut(s) 259, 391, 522, 812
Tsp45I GTSAC 4 cut(s) 259, 391, 522, 812
TspDTI ATGAA 5 cut(s) 245, 947, 1132, 1347, 1398
TspRI CASTG 2 cut(s) 1018, 1048
Tth111I GACNNNGTC 1 cut(s) 520
Van91I CCANNNNNTGG 1 cut(s) 1288
Vha464I CTTAAG 1 cut(s) 505
VpaK11BI GGWCC 2 cut(s) 241, 1429
XagI CCTNNNNNAGG 1 cut(s) 155
XapI RAATTY 5 cut(s) 474, 495, 1060, 1093, 1448
XbaI TCTAGA 1 cut(s) 175
XcmI CCANNNNNNNNNTGG 1 cut(s) 1289
XmnI GAANNNNTTC 1 cut(s) 1449
XspI CTAG 5 cut(s) 101, 176, 440, 552, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.