FvH4_3g07460

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013321
Physical Location & Seq
Reverse (-)
4415412 .. 4418569
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g07460.t1

Sequence Viewer

Length: 1005 bp
ATGGATGCAGGGTCACATGATGTCCAAGGTAGCAGTGTGGTGTTGGGTGGGGTTGATCAGAATACCCTTGAGAAATCAAGGAATTCTGTTGTTTGGTCAGTTGTTGAGTCTGTCATTGTTGAGGCGGCCACTGCTATTCGAAAAACTGAAGATTTGAAGGCATTAGGTGGTGAGTTAGGGTCTAATAAGGCAGTAGGTGCAGAAGCGGTTGAGAGACTGAATGATAAGGAGGCATGTGAGCAGCGTAATAGTAGTGATGTTTCGGGTCATGCTGAGCTTGGAACTAGTAAGAATTCAAGCAATTTGGCTAATGGAAATGTGTCTGGGAGTGTGGTTGTCATCGACTCTGGTGAGGCTTCTCGTGCTACTGGAGATGATGGAAGATTGGGTGTAGAGGGTGATGAACTGGGGTCAAATAAGGTGGATGAGCCAAAGATGAAGGTGCCTGAGAAAGAGAGTGATTTACATGTGATTAATATCAAGTGTGATGAAATGGGGTCGAGTAAGGTGGAAGAGTCAAAGATGAAGGTGCCTGAGGCAGAGAGGGATTCACATGTGATAAATATCAAATGTGGGAGTCGTAAAGGATTTGGTGATAATTGGGAGGGGGAAAGTGTTTGTAGGATTTGCCATTTAAGTTCTGATCAATCACCGGACAGAAAAACAGAAAGTAGTTCTTATTCTGTCTCGATGGATTTAATTCATCTTGGCTGTGGGTGTAAAGATGAATTAGGCATTGCACATGCTCATTGTGCTGAAGCATGGTTTAAGCTAAAAGGAAACAGAATGTGTGAAATTTGTGGTGAGAATGCAAAAAATGTTACAGGTCTTGGGGATAACAGATTTTTGGAGGAGTGGAATGAAAGAAGGTTTTCGGGTCTCGGCGCGAACACATCAGAAAGGAATGTAGGATGTTGGCGAGGACAGCCATTTTGCAATTTTTTAATGGCATGCCTGGTAATAGCTTTTGTGCTGCCATGGTTTTTTCGTGTAAATATGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

335

Amino Acids

36.14

Weight (kDa)

5.14

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 207 - 267 1.8e-06 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013321)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34200 AT2G34200
fragaria_vesca FvH4_3g07460
malus_domestica MD10G1277000.v1.1
prunus_persica Prupe.4G064900_v2.0.a1 Prupe.4G064900_v2.0.a1
pyrus_communis pycom05g27540 pycom10g23130
rosa_chinensis RchiOBHm_Chr5g0011391
rosa_laevigata RLG00000031829
rosa_multiflora Rmu_sc0009975.1_g000013
rosa_roxburghii Rroxscaffold_1G00065060
rosa_rugosa Rorug04G0456800
rosa_samantha Rh5AG088500 Rh5BG083600 Rh5CG096900 Rh5DG083500
rosa_wichuraiana Rw5G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 442, 529
AccII CGCG 1 cut(s) 887
AciI CCGC 2 cut(s) 125, 206
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 3 cut(s) 82, 292, 795
AcuI CTGAAG 2 cut(s) 168, 777
AflIII ACRYGT 2 cut(s) 466, 553
AgsI TTSAA 2 cut(s) 157, 297
AhlI ACTAGT 1 cut(s) 284
AjnI CCWGG 1 cut(s) 954
AluBI AGCT 3 cut(s) 277, 772, 965
AluI AGCT 3 cut(s) 277, 772, 965
Alw26I GTCTC 3 cut(s) 208, 691, 884
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 2 cut(s) 241, 973
ApoI RAATTY 3 cut(s) 82, 292, 795
ArsI GACNNNNNNTTYG 2 cut(s) 915, 947
AseI ATTAAT 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 871
AspLEI GCGC 1 cut(s) 887
AsuHPI GGTGA 6 cut(s) 182, 362, 410, 605, 642, 815
AsuII TTCGAA 1 cut(s) 139
AxyI CCTNAGG 1 cut(s) 534
BanI GGYRCC 2 cut(s) 442, 529
BauI CACGAG 1 cut(s) 360
BbvI GCAGC 2 cut(s) 253, 960
BccI CCATC 2 cut(s) 371, 685
BciT130I CCWGG 1 cut(s) 956
BclI TGATCA 2 cut(s) 55, 643
BcoDI GTCTC 3 cut(s) 208, 691, 884
BcuI ACTAGT 1 cut(s) 284
BfaI CTAG 2 cut(s) 285, 1003
BisI GCNGC 3 cut(s) 126, 242, 974
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 3 cut(s) 127, 243, 975
Bme1390I CCNGG 1 cut(s) 956
BmiI GGNNCC 2 cut(s) 444, 531
BmrFI CCNGG 1 cut(s) 956
BmrI ACTGGG 1 cut(s) 416
BmuI ACTGGG 1 cut(s) 416
BpmI CTGGAG 1 cut(s) 390
Bpu1102I GCTNAGC 1 cut(s) 273
Bpu14I TTCGAA 1 cut(s) 139
BpuEI CTTGAG 1 cut(s) 89
BsaBI GATNNNNATC 2 cut(s) 476, 563
BsaI GGTCTC 1 cut(s) 884
BsaJI CCNNGG 2 cut(s) 25, 977
BsaWI WCCGGW 1 cut(s) 652
Bse1I ACTGG 2 cut(s) 373, 411
Bse21I CCTNAGG 1 cut(s) 534
Bse3DI GCAATG 1 cut(s) 735
Bse8I GATNNNNATC 2 cut(s) 476, 563
BseBI CCWGG 1 cut(s) 956
BseDI CCNNGG 2 cut(s) 25, 977
BseGI GGATG 3 cut(s) 10, 430, 917
BseJI GATNNNNATC 2 cut(s) 476, 563
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 3 cut(s) 264, 438, 525
BseNI ACTGG 2 cut(s) 373, 411
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 2 cut(s) 253, 960
BsgI GTGCAG 1 cut(s) 219
Bsh1236I CGCG 1 cut(s) 887
BshFI GGCC 1 cut(s) 128
BshNI GGYRCC 2 cut(s) 442, 529
BsiSI CCGG 1 cut(s) 653
BsmAI GTCTC 3 cut(s) 208, 691, 884
BsmI GAATGC 1 cut(s) 814
BsnI GGCC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 884
Bsp119I TTCGAA 1 cut(s) 139
Bsp143I GATC 2 cut(s) 55, 643
Bsp1720I GCTNAGC 1 cut(s) 273
Bsp19I CCATGG 1 cut(s) 977
BspACI CCGC 2 cut(s) 125, 206
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 3 cut(s) 265, 439, 526
BspFNI CGCG 1 cut(s) 887
BspLI GGNNCC 2 cut(s) 444, 531
BspT104I TTCGAA 1 cut(s) 139
BspT107I GGYRCC 2 cut(s) 442, 529
BspTNI GGTCTC 1 cut(s) 884
BsrDI GCAATG 1 cut(s) 735
BsrI ACTGG 2 cut(s) 373, 411
BssECI CCNNGG 2 cut(s) 25, 977
BssMI GATC 2 cut(s) 55, 643
BssSI CACGAG 1 cut(s) 360
BssT1I CCWWGG 2 cut(s) 25, 977
Bst2BI CACGAG 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 956
Bst6I CTCTTC 1 cut(s) 507
BstAPI GCANNNNNTGC 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 139
BstC8I GCNNGC 1 cut(s) 952
BstDEI CTNAG 3 cut(s) 273, 447, 534
BstDSI CCRYGG 1 cut(s) 977
BstF5I GGATG 3 cut(s) 10, 430, 917
BstFNI CGCG 1 cut(s) 887
BstHHI GCGC 1 cut(s) 887
BstKTI GATC 2 cut(s) 58, 646
BstMAI GTCTC 3 cut(s) 208, 691, 884
BstMBI GATC 2 cut(s) 55, 643
BstMWI GCNNNNNNNGC 5 cut(s) 131, 197, 362, 752, 925
BstNI CCWGG 1 cut(s) 956
BstNSI RCATGY 5 cut(s) 237, 470, 557, 746, 954
BstSCI CCNGG 1 cut(s) 954
BstUI CGCG 1 cut(s) 887
BstV1I GCAGC 2 cut(s) 253, 960
Bsu36I CCTNAGG 1 cut(s) 534
BsuRI GGCC 1 cut(s) 128
BtgI CCRYGG 1 cut(s) 977
BtsCI GGATG 3 cut(s) 10, 430, 917
BtsI GCAGTG 2 cut(s) 40, 129
BtsIMutI CAGTG 2 cut(s) 40, 129
Cac8I GCNNGC 1 cut(s) 952
CfoI GCGC 1 cut(s) 887
CspCI CAANNNNNGTGG 2 cut(s) 402, 437
CviAII CATG 9 cut(s) 17, 234, 269, 467, 554, 743, 762, 951, 978
CviJI RGCY 9 cut(s) 128, 277, 308, 356, 430, 711, 772, 928, 965
CviKI_1 RGCY 9 cut(s) 128, 277, 308, 356, 430, 711, 772, 928, 965
DdeI CTNAG 3 cut(s) 273, 447, 534
DpnI GATC 2 cut(s) 57, 645
DpnII GATC 2 cut(s) 55, 643
EaeI YGGCCR 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
Eco130I CCWWGG 2 cut(s) 25, 977
Eco31I GGTCTC 1 cut(s) 884
Eco57I CTGAAG 2 cut(s) 168, 777
Eco81I CCTNAGG 1 cut(s) 534
EcoRI GAATTC 2 cut(s) 82, 292
EcoRII CCWGG 1 cut(s) 954
EcoT14I CCWWGG 2 cut(s) 25, 977
ErhI CCWWGG 2 cut(s) 25, 977
FaeI CATG 9 cut(s) 20, 237, 272, 470, 557, 746, 765, 954, 981
FalI AAGNNNNNCTT 2 cut(s) 661, 693
FatI CATG 9 cut(s) 16, 233, 268, 466, 553, 742, 761, 950, 977
FbaI TGATCA 2 cut(s) 55, 643
Fnu4HI GCNGC 3 cut(s) 126, 242, 974
FokI GGATG 3 cut(s) 17, 437, 924
Fsp4HI GCNGC 3 cut(s) 126, 242, 974
FspBI CTAG 2 cut(s) 285, 1003
GlaI GCGC 1 cut(s) 886
GluI GCNGC 3 cut(s) 126, 242, 974
GsuI CTGGAG 1 cut(s) 390
HaeIII GGCC 1 cut(s) 128
HapII CCGG 1 cut(s) 653
HhaI GCGC 1 cut(s) 887
Hin1II CATG 9 cut(s) 20, 237, 272, 470, 557, 746, 765, 954, 981
Hin6I GCGC 1 cut(s) 885
HinP1I GCGC 1 cut(s) 885
HinfI GANTC 5 cut(s) 107, 344, 515, 548, 577
HpaII CCGG 1 cut(s) 653
HphI GGTGA 6 cut(s) 182, 362, 410, 605, 642, 815
Hpy188I TCNGA 3 cut(s) 60, 643, 898
Hpy188III TCNNGA 1 cut(s) 688
HpyAV CCTTC 4 cut(s) 151, 433, 520, 861
HpyCH4V TGCA 5 cut(s) 8, 200, 740, 812, 936
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 197, 362, 752, 925
HpyF3I CTNAG 3 cut(s) 273, 447, 534
Hsp92II CATG 9 cut(s) 20, 237, 272, 470, 557, 746, 765, 954, 981
HspAI GCGC 1 cut(s) 885
Ksp22I TGATCA 2 cut(s) 55, 643
Kzo9I GATC 2 cut(s) 55, 643
Lsp1109I GCAGC 2 cut(s) 253, 960
MaeI CTAG 2 cut(s) 285, 1003
MaeIII GTNAC 2 cut(s) 12, 820
MalI GATC 2 cut(s) 57, 645
MboI GATC 2 cut(s) 55, 643
MboII GAAGA 3 cut(s) 161, 393, 524
MluCI AATT 8 cut(s) 82, 292, 301, 598, 699, 728, 795, 937
MlyI GAGTC 4 cut(s) 116, 338, 524, 586
MnlI CCTC 9 cut(s) 115, 223, 346, 388, 529, 537, 598, 844, 914
MroXI GAANNNNTTC 1 cut(s) 871
MseI TTAA 5 cut(s) 474, 635, 698, 768, 944
MspI CCGG 1 cut(s) 653
MspR9I CCNGG 1 cut(s) 956
Mva1269I GAATGC 1 cut(s) 814
MvaI CCWGG 1 cut(s) 956
MvnI CGCG 1 cut(s) 887
MwoI GCNNNNNNNGC 5 cut(s) 131, 197, 362, 752, 925
NcoI CCATGG 1 cut(s) 977
NdeII GATC 2 cut(s) 55, 643
NlaIII CATG 9 cut(s) 20, 237, 272, 470, 557, 746, 765, 954, 981
NlaIV GGNNCC 2 cut(s) 444, 531
NmeAIII GCCGAG 1 cut(s) 861
NmuCI GTSAC 1 cut(s) 12
NspI RCATGY 5 cut(s) 237, 470, 557, 746, 954
NspV TTCGAA 1 cut(s) 139
PaeI GCATGC 1 cut(s) 954
PciI ACATGT 2 cut(s) 466, 553
PctI GAATGC 1 cut(s) 814
PdmI GAANNNNTTC 1 cut(s) 871
PfeI GAWTC 1 cut(s) 548
PkrI GCNGC 3 cut(s) 127, 243, 975
PleI GAGTC 4 cut(s) 115, 338, 523, 585
PpsI GAGTC 4 cut(s) 115, 338, 523, 585
PscI ACATGT 2 cut(s) 466, 553
PshBI ATTAAT 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 954
PspGI CCWGG 1 cut(s) 954
PspN4I GGNNCC 2 cut(s) 444, 531
SaqAI TTAA 5 cut(s) 474, 635, 698, 768, 944
SatI GCNGC 3 cut(s) 126, 242, 974
Sau3AI GATC 2 cut(s) 55, 643
SchI GAGTC 4 cut(s) 116, 338, 524, 586
ScrFI CCNGG 1 cut(s) 956
SfuI TTCGAA 1 cut(s) 139
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
SpeI ACTAGT 1 cut(s) 284
SphI GCATGC 1 cut(s) 954
Sse9I AATT 8 cut(s) 82, 292, 301, 598, 699, 728, 795, 937
SsiI CCGC 2 cut(s) 125, 206
SspMI CTAG 2 cut(s) 285, 1003
StyD4I CCNGG 1 cut(s) 954
StyI CCWWGG 2 cut(s) 25, 977
TaqI TCGA 4 cut(s) 139, 342, 500, 689
TasI AATT 8 cut(s) 82, 292, 301, 598, 699, 728, 795, 937
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 5 cut(s) 474, 635, 698, 768, 944
Tru9I TTAA 5 cut(s) 474, 635, 698, 768, 944
TscAI CASTG 2 cut(s) 40, 136
TseFI GTSAC 1 cut(s) 12
TseI GCWGC 2 cut(s) 241, 973
Tsp45I GTSAC 1 cut(s) 12
TspDTI ATGAA 7 cut(s) 417, 452, 504, 539, 692, 741, 876
TspRI CASTG 2 cut(s) 40, 136
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 3 cut(s) 82, 292, 795
XceI RCATGY 5 cut(s) 237, 470, 557, 746, 954
XmnI GAANNNNTTC 1 cut(s) 871
XspI CTAG 2 cut(s) 285, 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.