MD10G1277000.v1.1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013321
Physical Location & Seq
Reverse (-)
36790417 .. 36793354
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1277000.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGATGCCGGGGCATGCGATTTGCAGGATAGGAGGGAGGTGTTGGGTGGAGTTGATCAAAAGGGGGGCGAAAAGTCGAAAAGTTCTGTTGTATGGTCGGTTGTCGAGACTGTTATTGTGATTGAATCCGAAGAGGATGTTTGTGTCACCGGAAAAAATGAAGATTTGGAAGCACTGGGGAGTGAGTTGGGGTCTGATAAAGCAGTGGTAGAAGGGCTTGAGAAGCCGAATGATAAGGAGGCGTGCGGCCAGCGTGGTAGTGATGAGGTACCTAGTCAAGTTGAGCAAGGAGCTAATAAGAATTCGAACTGTTCGGTTGATGGGAATGTATATGAGACCGTGGTTGTCATTAATTCAAGTGAGTCTGGGAGTGCAGTTGGAGATAACAGAAGATTGGTTCCGAAGGTTGAGGAATTGGGATCGAGTAAGGTATTAATGGAAGAGTCGAAGAAGAAGGTGCCTCAAGCAGAAAATGATTCATATGTGAATGATATGAAGTGTGGGAGTGGTAAAGGGTTTGGTAATAAATGGGACGGGGAAAGGGTTTGTAGGATTTGCCATCTAAATTCTGATATCTCACCGCCGAGAAAAATGGGAACTGCTAATAGTTCGATCACGGCAGATTTAATTCAACTCGGTTGTGGGTGTAAAGATGAGCTTGGCGTTGCTCATGGTCATTGTGCCGAAGCATGGTTTAGGGTAAAAGGAAACAGGACGTGTGAAATATGTGGAGAGACTGCGCAAAACATTACCGGTCATGGGAATAACAGATTTATGGAGGAGTGGCATGAAGCAACGCTTGCTGATATCGGTAATAACTCATCGGAGACGAATGCATCCTGTTGGCGGGGACAACCATTTTGCAACTTCATAATGGCATGTTTGGTGATAGCTTTTGTACTTCCATGGTTTTTTCGTGTGAATCTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

311

Amino Acids

33.55

Weight (kDa)

4.83

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 183 - 243 2.7e-06 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013321)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34200 AT2G34200
fragaria_vesca FvH4_3g07460
malus_domestica MD10G1277000.v1.1
prunus_persica Prupe.4G064900_v2.0.a1 Prupe.4G064900_v2.0.a1
pyrus_communis pycom05g27540 pycom10g23130
rosa_chinensis RchiOBHm_Chr5g0011391
rosa_laevigata RLG00000031829
rosa_multiflora Rmu_sc0009975.1_g000013
rosa_roxburghii Rroxscaffold_1G00065060
rosa_rugosa Rorug04G0456800
rosa_samantha Rh5AG088500 Rh5BG083600 Rh5CG096900 Rh5DG083500
rosa_wichuraiana Rw5G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 741
Acc65I GGTACC 1 cut(s) 268
AccB1I GGYRCC 2 cut(s) 268, 457
AciI CCGC 3 cut(s) 246, 581, 847
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 2 cut(s) 301, 565
AfaI GTAC 2 cut(s) 270, 900
AfiI CCNNNNNNNGG 3 cut(s) 690, 759, 846
AflIII ACRYGT 1 cut(s) 716
AgeI ACCGGT 1 cut(s) 752
AgsI TTSAA 3 cut(s) 125, 357, 632
AjiI CACGTC 1 cut(s) 717
AluBI AGCT 3 cut(s) 293, 658, 893
AluI AGCT 3 cut(s) 293, 658, 893
Alw26I GTCTC 4 cut(s) 101, 329, 728, 821
AlwI GGATC 1 cut(s) 427
AoxI GGCC 1 cut(s) 247
ApoI RAATTY 2 cut(s) 301, 565
ArsI GACNNNNNNTTYG 2 cut(s) 843, 875
AseI ATTAAT 2 cut(s) 351, 434
AsiGI ACCGGT 1 cut(s) 752
Asp700I GAANNNNTTC 1 cut(s) 926
Asp718I GGTACC 1 cut(s) 268
AspLEI GCGC 1 cut(s) 742
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 3 cut(s) 139, 570, 898
AsuII TTCGAA 1 cut(s) 305
BanI GGYRCC 2 cut(s) 268, 457
BccI CCATC 2 cut(s) 314, 567
BceAI ACGGC 1 cut(s) 633
BclI TGATCA 1 cut(s) 55
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 4 cut(s) 101, 329, 728, 821
BfaI CTAG 2 cut(s) 273, 931
BisI GCNGC 1 cut(s) 247
BlsI GCNGC 1 cut(s) 248
Bme1390I CCNGG 1 cut(s) 10
BmgBI CACGTC 1 cut(s) 717
BmiI GGNNCC 3 cut(s) 270, 399, 459
BmrFI CCNGG 1 cut(s) 10
BmrI ACTGGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 845
BmuI ACTGGG 1 cut(s) 185
Bpu14I TTCGAA 1 cut(s) 305
BpuEI CTTGAG 2 cut(s) 239, 447
BpuMI CCSGG 1 cut(s) 10
BsaI GGTCTC 1 cut(s) 329
BsaJI CCNNGG 3 cut(s) 9, 339, 905
BsaWI WCCGGW 2 cut(s) 149, 752
Bsc4I CCNNNNNNNGG 3 cut(s) 690, 759, 846
Bse118I RCCGGY 1 cut(s) 752
Bse1I ACTGG 1 cut(s) 180
BseDI CCNNGG 3 cut(s) 9, 339, 905
BseGI GGATG 3 cut(s) 10, 142, 836
BseLI CCNNNNNNNGG 3 cut(s) 690, 759, 846
BseNI ACTGG 1 cut(s) 180
BseRI GAGGAG 1 cut(s) 794
BsgI GTGCAG 1 cut(s) 393
BshFI GGCC 1 cut(s) 249
BshNI GGYRCC 2 cut(s) 268, 457
BshTI ACCGGT 1 cut(s) 752
BsiSI CCGG 3 cut(s) 9, 150, 753
BslFI GGGAC 2 cut(s) 545, 864
BslI CCNNNNNNNGG 3 cut(s) 690, 759, 846
BsmAI GTCTC 4 cut(s) 101, 329, 728, 821
BsmBI CGTCTC 1 cut(s) 821
BsmFI GGGAC 2 cut(s) 545, 864
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 1 cut(s) 249
Bso31I GGTCTC 1 cut(s) 329
Bsp119I TTCGAA 1 cut(s) 305
Bsp143I GATC 3 cut(s) 55, 419, 612
Bsp19I CCATGG 1 cut(s) 905
BspACI CCGC 3 cut(s) 246, 581, 847
BspANI GGCC 1 cut(s) 249
BspLI GGNNCC 3 cut(s) 270, 399, 459
BspPI GGATC 1 cut(s) 427
BspT104I TTCGAA 1 cut(s) 305
BspT107I GGYRCC 2 cut(s) 268, 457
BspTNI GGTCTC 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 752
BsrI ACTGG 1 cut(s) 180
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 3 cut(s) 9, 339, 905
BssMI GATC 3 cut(s) 55, 419, 612
BssT1I CCWWGG 1 cut(s) 905
Bst4CI ACNGT 3 cut(s) 112, 311, 340
Bst6I CTCTTC 2 cut(s) 126, 435
BstAPI GCANNNNNTGC 1 cut(s) 800
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 16, 244, 251, 801
BstDSI CCRYGG 2 cut(s) 339, 905
BstF5I GGATG 3 cut(s) 10, 142, 836
BstHHI GCGC 1 cut(s) 742
BstKTI GATC 3 cut(s) 58, 422, 615
BstMAI GTCTC 4 cut(s) 101, 329, 728, 821
BstMBI GATC 3 cut(s) 55, 419, 612
BstMWI GCNNNNNNNGC 2 cut(s) 223, 800
BstNSI RCATGY 2 cut(s) 18, 882
BstSCI CCNGG 1 cut(s) 8
BsuRI GGCC 1 cut(s) 249
BtgI CCRYGG 2 cut(s) 339, 905
BtrI CACGTC 1 cut(s) 717
BtsCI GGATG 3 cut(s) 10, 142, 836
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 2 cut(s) 173, 210
Cac8I GCNNGC 4 cut(s) 16, 244, 251, 801
CfoI GCGC 1 cut(s) 742
Cfr10I RCCGGY 1 cut(s) 752
Csp6I GTAC 2 cut(s) 269, 899
CspAI ACCGGT 1 cut(s) 752
CviAII CATG 7 cut(s) 15, 671, 690, 758, 788, 879, 906
CviJI RGCY 6 cut(s) 217, 226, 249, 293, 658, 893
CviKI_1 RGCY 6 cut(s) 217, 226, 249, 293, 658, 893
CviQI GTAC 2 cut(s) 269, 899
DpnI GATC 3 cut(s) 57, 421, 614
DpnII GATC 3 cut(s) 55, 419, 612
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 2 cut(s) 126, 435
EarI CTCTTC 2 cut(s) 126, 435
Eco130I CCWWGG 1 cut(s) 905
Eco31I GGTCTC 1 cut(s) 329
Eco32I GATATC 2 cut(s) 574, 808
EcoRI GAATTC 1 cut(s) 301
EcoRV GATATC 2 cut(s) 574, 808
EcoT14I CCWWGG 1 cut(s) 905
EcoT22I ATGCAT 1 cut(s) 838
ErhI CCWWGG 1 cut(s) 905
Esp3I CGTCTC 1 cut(s) 821
FaeI CATG 7 cut(s) 18, 674, 693, 761, 791, 882, 909
FalI AAGNNNNNCTT 4 cut(s) 642, 674, 783, 815
FaqI GGGAC 2 cut(s) 545, 864
FatI CATG 7 cut(s) 14, 670, 689, 757, 787, 878, 905
FauI CCCGC 1 cut(s) 840
FauNDI CATATG 1 cut(s) 481
FbaI TGATCA 1 cut(s) 55
Fnu4HI GCNGC 1 cut(s) 247
FokI GGATG 3 cut(s) 17, 149, 823
Fsp4HI GCNGC 1 cut(s) 247
FspBI CTAG 2 cut(s) 273, 931
FspI TGCGCA 1 cut(s) 741
GlaI GCGC 1 cut(s) 741
GluI GCNGC 1 cut(s) 247
HaeIII GGCC 1 cut(s) 249
HapII CCGG 3 cut(s) 9, 150, 753
HhaI GCGC 1 cut(s) 742
Hin1II CATG 7 cut(s) 18, 674, 693, 761, 791, 882, 909
Hin6I GCGC 1 cut(s) 740
HinP1I GCGC 1 cut(s) 740
HinfI GANTC 5 cut(s) 125, 362, 443, 476, 922
HpaII CCGG 3 cut(s) 9, 150, 753
HphI GGTGA 3 cut(s) 139, 570, 898
Hpy188I TCNGA 5 cut(s) 130, 196, 402, 571, 826
Hpy188III TCNNGA 1 cut(s) 106
HpyAV CCTTC 3 cut(s) 206, 397, 448
HpyCH4III ACNGT 3 cut(s) 112, 311, 340
HpyCH4IV ACGT 1 cut(s) 716
HpyCH4V TGCA 4 cut(s) 25, 374, 836, 864
HpyF10VI GCNNNNNNNGC 2 cut(s) 223, 800
HpySE526I ACGT 1 cut(s) 716
Hsp92II CATG 7 cut(s) 18, 674, 693, 761, 791, 882, 909
HspAI GCGC 1 cut(s) 740
KpnI GGTACC 1 cut(s) 272
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 3 cut(s) 55, 419, 612
LmnI GCTCC 1 cut(s) 290
LpnPI CCDG 9 cut(s) 11, 22, 161, 163, 263, 351, 697, 766, 853
LweI GCATC 1 cut(s) 845
MaeI CTAG 2 cut(s) 273, 931
MaeII ACGT 1 cut(s) 716
MaeIII GTNAC 1 cut(s) 145
MalI GATC 3 cut(s) 57, 421, 614
MboI GATC 3 cut(s) 55, 419, 612
MboII GAAGA 6 cut(s) 143, 173, 402, 452, 460, 463
MluCI AATT 5 cut(s) 301, 352, 413, 565, 627
MlyI GAGTC 2 cut(s) 371, 452
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 8 cut(s) 27, 31, 127, 232, 259, 403, 471, 772
Mph1103I ATGCAT 1 cut(s) 838
MroXI GAANNNNTTC 1 cut(s) 926
MseI TTAA 3 cut(s) 351, 434, 626
MspI CCGG 3 cut(s) 9, 150, 753
MspR9I CCNGG 1 cut(s) 10
Mva1269I GAATGC 1 cut(s) 838
MwoI GCNNNNNNNGC 2 cut(s) 223, 800
NciI CCSGG 1 cut(s) 10
NcoI CCATGG 1 cut(s) 905
NdeI CATATG 1 cut(s) 481
NdeII GATC 3 cut(s) 55, 419, 612
NlaIII CATG 7 cut(s) 18, 674, 693, 761, 791, 882, 909
NlaIV GGNNCC 3 cut(s) 270, 399, 459
NmeAIII GCCGAG 1 cut(s) 609
NmuCI GTSAC 1 cut(s) 145
NsbI TGCGCA 1 cut(s) 741
NsiI ATGCAT 1 cut(s) 838
NspI RCATGY 2 cut(s) 18, 882
NspV TTCGAA 1 cut(s) 305
PaeI GCATGC 1 cut(s) 18
PctI GAATGC 1 cut(s) 838
PdmI GAANNNNTTC 1 cut(s) 926
PfeI GAWTC 3 cut(s) 125, 476, 922
PinAI ACCGGT 1 cut(s) 752
PkrI GCNGC 1 cut(s) 248
PleI GAGTC 2 cut(s) 370, 451
PpsI GAGTC 2 cut(s) 370, 451
PshBI ATTAAT 2 cut(s) 351, 434
PspN4I GGNNCC 3 cut(s) 270, 399, 459
RsaI GTAC 2 cut(s) 270, 900
RsaNI GTAC 2 cut(s) 269, 899
SaqAI TTAA 3 cut(s) 351, 434, 626
SatI GCNGC 1 cut(s) 247
Sau3AI GATC 3 cut(s) 55, 419, 612
SchI GAGTC 2 cut(s) 371, 452
ScrFI CCNGG 1 cut(s) 10
SfaNI GCATC 1 cut(s) 845
SfuI TTCGAA 1 cut(s) 305
SmlI CTYRAG 2 cut(s) 218, 462
SmoI CTYRAG 2 cut(s) 218, 462
SphI GCATGC 1 cut(s) 18
Sse9I AATT 5 cut(s) 301, 352, 413, 565, 627
SsiI CCGC 3 cut(s) 246, 581, 847
SspMI CTAG 2 cut(s) 273, 931
StyD4I CCNGG 1 cut(s) 8
StyI CCWWGG 1 cut(s) 905
TaaI ACNGT 3 cut(s) 112, 311, 340
TaiI ACGT 1 cut(s) 719
TaqI TCGA 6 cut(s) 77, 105, 305, 422, 446, 611
TasI AATT 5 cut(s) 301, 352, 413, 565, 627
TatI WGTACW 1 cut(s) 898
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 3 cut(s) 125, 476, 922
Tru1I TTAA 3 cut(s) 351, 434, 626
Tru9I TTAA 3 cut(s) 351, 434, 626
TscAI CASTG 2 cut(s) 180, 210
TseFI GTSAC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 5 cut(s) 174, 468, 509, 804, 859
TspRI CASTG 2 cut(s) 180, 210
VspI ATTAAT 2 cut(s) 351, 434
XapI RAATTY 2 cut(s) 301, 565
XceI RCATGY 2 cut(s) 18, 882
XmnI GAANNNNTTC 1 cut(s) 926
XspI CTAG 2 cut(s) 273, 931
Zsp2I ATGCAT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.