Rroxscaffold_1G00065060

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013321
Physical Location & Seq
Reverse (-)
86830515 .. 86833472
2958 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00065060.1

Sequence Viewer

Length: 918 bp
ATGGATGCAGGGTCACATGATGTTCAAGGTAGCAGTGTGGTATTGGGTGGGGTTGATCAGAATTCCCTTGAGAAATCAAGGAATTCTGTTGTTTGGTCAGTTGTTGAGTCTGTCATTGTCGAGGCGGCCACTGCTATTCGGAAAACTGAAGATTTGAAGGCATTGGGTGGTGAATTAGGGTCTAATAAGGCAGTAGGTGGAGAAGGGGTTGAGAGACTGAATAATAAGGAGGCATGTGAGCCGCGTACTAGTAGTGAGGTTTCGAGTCATGTTGAGCTAGAAACTAATAAGAATTTGAACAATTTAGCTGATGGGAATGTGTCTGACACTGTTGTTGCCATCGACTCTGGTGAGGCTTCACGTGGTACTGGAGATGATGGAAGATTGGGTGTAAGGGGTGATGAACTGGGGTCGACTAAGGTGGCAGAGTCAAAGATGAAGGTGCCTGAGGCAGAGAGGGATTCACATGTGATTAATATCAAGTGTGGGAATGGTAAAGGATTTGGTGATAACTGGGAGGCGGAAAGTGTTTGTAGGATTTGCCATTTAAGTTCTGATCAATCGCCGGACAGAAAAACAGAATCTAGTTCTTGTTCTGTTACTATGGATTTAATTCATCTTGGCTGTGAGTGTAAAGATGAATTAGGCGTTGCACATGCTCATTGTGCTGAAGCTTGGTTTAAGCTAAAAGGAAACAGAATGTGTGAAATTTGTGGCGATACTGCAAAAAATGTTACTGGTCTTGGGGATAACAGATTTTTGGAGGAGTGGAACGAAAGAAGATTTTCTGGTCTTGGTGCGAACACATCAGAGAGGAATGTTGGATGTTGGCGGGGACAACCATTTTGCAATTTCTTAATGGCATGCTTGGTAATAGCTTTTGTGCTGCCGTGGTTTTTTCGTGTAAATATGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

305

Amino Acids

32.92

Weight (kDa)

5.09

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 178 - 238 5.3e-06 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013321)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34200 AT2G34200
fragaria_vesca FvH4_3g07460
malus_domestica MD10G1277000.v1.1
prunus_persica Prupe.4G064900_v2.0.a1 Prupe.4G064900_v2.0.a1
pyrus_communis pycom05g27540 pycom10g23130
rosa_chinensis RchiOBHm_Chr5g0011391
rosa_laevigata RLG00000031829
rosa_multiflora Rmu_sc0009975.1_g000013
rosa_roxburghii Rroxscaffold_1G00065060
rosa_rugosa Rorug04G0456800
rosa_samantha Rh5AG088500 Rh5BG083600 Rh5CG096900 Rh5DG083500
rosa_wichuraiana Rw5G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 442
AccI GTMKAC 1 cut(s) 413
AccII CGCG 1 cut(s) 244
AciI CCGC 4 cut(s) 125, 242, 521, 832
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 4 cut(s) 61, 82, 292, 708
AcuI CTGAAG 2 cut(s) 168, 690
AcvI CACGTG 1 cut(s) 362
AfaI GTAC 2 cut(s) 247, 367
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 3 cut(s) 26, 157, 298
AhlI ACTAGT 1 cut(s) 248
AluBI AGCT 5 cut(s) 277, 308, 674, 685, 878
AluI AGCT 5 cut(s) 277, 308, 674, 685, 878
Alw26I GTCTC 1 cut(s) 208
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 886
ApoI RAATTY 4 cut(s) 61, 82, 292, 708
ArsI GACNNNNNNTTYG 2 cut(s) 828, 860
AseI ATTAAT 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 784
AsuHPI GGTGA 4 cut(s) 182, 362, 410, 518
AxyI CCTNAGG 1 cut(s) 447
BanI GGYRCC 1 cut(s) 442
BbrPI CACGTG 1 cut(s) 362
BbvI GCAGC 1 cut(s) 873
BccI CCATC 3 cut(s) 305, 347, 371
BceAI ACGGC 1 cut(s) 874
BclI TGATCA 2 cut(s) 55, 556
BcoDI GTCTC 1 cut(s) 208
BcuI ACTAGT 1 cut(s) 248
BfaI CTAG 4 cut(s) 249, 278, 585, 916
BisI GCNGC 3 cut(s) 126, 242, 887
BlsI GCNGC 3 cut(s) 127, 243, 888
BmiI GGNNCC 1 cut(s) 444
BmrI ACTGGG 2 cut(s) 416, 523
BmuI ACTGGG 2 cut(s) 416, 523
BpmI CTGGAG 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 89
BsaAI YACGTR 1 cut(s) 362
BsaBI GATNNNNATC 1 cut(s) 476
BsaJI CCNNGG 1 cut(s) 890
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bse1I ACTGG 4 cut(s) 373, 411, 518, 742
Bse21I CCTNAGG 1 cut(s) 447
Bse8I GATNNNNATC 1 cut(s) 476
BseDI CCNNGG 1 cut(s) 890
BseGI GGATG 2 cut(s) 10, 830
BseJI GATNNNNATC 1 cut(s) 476
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 4 cut(s) 373, 411, 518, 742
BseRI GAGGAG 1 cut(s) 779
BseXI GCAGC 1 cut(s) 873
Bsh1236I CGCG 1 cut(s) 244
BshFI GGCC 1 cut(s) 128
BshNI GGYRCC 1 cut(s) 442
BsiSI CCGG 1 cut(s) 566
BslFI GGGAC 1 cut(s) 849
BsmAI GTCTC 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 849
BsnI GGCC 1 cut(s) 128
Bsp143I GATC 2 cut(s) 55, 556
BspACI CCGC 4 cut(s) 125, 242, 521, 832
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 1 cut(s) 439
BspFNI CGCG 1 cut(s) 244
BspLI GGNNCC 1 cut(s) 444
BspT107I GGYRCC 1 cut(s) 442
BsrI ACTGG 4 cut(s) 373, 411, 518, 742
BssECI CCNNGG 1 cut(s) 890
BssMI GATC 2 cut(s) 55, 556
Bst4CI ACNGT 1 cut(s) 331
BstBAI YACGTR 1 cut(s) 362
BstC8I GCNNGC 1 cut(s) 865
BstDEI CTNAG 2 cut(s) 417, 447
BstDSI CCRYGG 1 cut(s) 890
BstF5I GGATG 2 cut(s) 10, 830
BstFNI CGCG 1 cut(s) 244
BstKTI GATC 2 cut(s) 58, 559
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 2 cut(s) 55, 556
BstMWI GCNNNNNNNGC 2 cut(s) 131, 665
BstNSI RCATGY 4 cut(s) 237, 470, 659, 867
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 1 cut(s) 873
Bsu36I CCTNAGG 1 cut(s) 447
BsuRI GGCC 1 cut(s) 128
BtgI CCRYGG 1 cut(s) 890
BtsCI GGATG 2 cut(s) 10, 830
BtsI GCAGTG 2 cut(s) 40, 129
BtsIMutI CAGTG 3 cut(s) 40, 129, 327
Cac8I GCNNGC 1 cut(s) 865
Csp6I GTAC 2 cut(s) 246, 366
CviAII CATG 6 cut(s) 17, 234, 269, 467, 656, 864
CviJI RGCY 9 cut(s) 128, 241, 277, 308, 356, 624, 674, 685, 878
CviKI_1 RGCY 9 cut(s) 128, 241, 277, 308, 356, 624, 674, 685, 878
CviQI GTAC 2 cut(s) 246, 366
DdeI CTNAG 2 cut(s) 417, 447
DpnI GATC 2 cut(s) 57, 558
DpnII GATC 2 cut(s) 55, 556
EaeI YGGCCR 1 cut(s) 126
EciI GGCGGA 1 cut(s) 536
Eco57I CTGAAG 2 cut(s) 168, 690
Eco72I CACGTG 1 cut(s) 362
Eco81I CCTNAGG 1 cut(s) 447
EcoRI GAATTC 2 cut(s) 61, 82
FaeI CATG 6 cut(s) 20, 237, 272, 470, 659, 867
FaiI YATR 8 cut(s) 18, 235, 270, 468, 605, 657, 865, 911
FaqI GGGAC 1 cut(s) 849
FatI CATG 6 cut(s) 16, 233, 268, 466, 655, 863
FauI CCCGC 1 cut(s) 825
FbaI TGATCA 2 cut(s) 55, 556
FblI GTMKAC 1 cut(s) 413
Fnu4HI GCNGC 3 cut(s) 126, 242, 887
FokI GGATG 2 cut(s) 17, 837
Fsp4HI GCNGC 3 cut(s) 126, 242, 887
FspBI CTAG 4 cut(s) 249, 278, 585, 916
GluI GCNGC 3 cut(s) 126, 242, 887
GsuI CTGGAG 1 cut(s) 390
HaeIII GGCC 1 cut(s) 128
HapII CCGG 1 cut(s) 566
Hin1II CATG 6 cut(s) 20, 237, 272, 470, 659, 867
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HindIII AAGCTT 1 cut(s) 672
HinfI GANTC 6 cut(s) 107, 265, 344, 428, 461, 581
HpaII CCGG 1 cut(s) 566
HphI GGTGA 4 cut(s) 182, 362, 410, 518
Hpy166II GTNNAC 1 cut(s) 414
Hpy188I TCNGA 5 cut(s) 60, 141, 325, 556, 811
Hpy8I GTNNAC 1 cut(s) 414
HpyAV CCTTC 3 cut(s) 151, 197, 433
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 4 cut(s) 8, 653, 725, 849
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 665
HpyF3I CTNAG 2 cut(s) 417, 447
HpySE526I ACGT 1 cut(s) 361
Hsp92II CATG 6 cut(s) 20, 237, 272, 470, 659, 867
Ksp22I TGATCA 2 cut(s) 55, 556
Kzo9I GATC 2 cut(s) 55, 556
LpnPI CCDG 8 cut(s) 333, 354, 392, 459, 499, 579, 723, 774
Lsp1109I GCAGC 1 cut(s) 873
MaeI CTAG 4 cut(s) 249, 278, 585, 916
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 3 cut(s) 12, 598, 733
MalI GATC 2 cut(s) 57, 558
MboI GATC 2 cut(s) 55, 556
MboII GAAGA 3 cut(s) 161, 393, 792
MluCI AATT 9 cut(s) 61, 82, 173, 292, 301, 612, 641, 708, 850
MlyI GAGTC 4 cut(s) 116, 274, 338, 437
MmeI TCCRAC 1 cut(s) 802
MnlI CCTC 9 cut(s) 115, 223, 250, 346, 442, 450, 511, 757, 807
MroXI GAANNNNTTC 1 cut(s) 784
MseI TTAA 5 cut(s) 474, 548, 611, 681, 857
MspI CCGG 1 cut(s) 566
MvnI CGCG 1 cut(s) 244
MwoI GCNNNNNNNGC 2 cut(s) 131, 665
NdeII GATC 2 cut(s) 55, 556
NlaIII CATG 6 cut(s) 20, 237, 272, 470, 659, 867
NlaIV GGNNCC 1 cut(s) 444
NmuCI GTSAC 1 cut(s) 12
NspI RCATGY 4 cut(s) 237, 470, 659, 867
PaeI GCATGC 1 cut(s) 867
PciI ACATGT 1 cut(s) 466
PdmI GAANNNNTTC 1 cut(s) 784
PfeI GAWTC 2 cut(s) 461, 581
PkrI GCNGC 3 cut(s) 127, 243, 888
PleI GAGTC 4 cut(s) 115, 273, 338, 436
PmaCI CACGTG 1 cut(s) 362
PmlI CACGTG 1 cut(s) 362
PpsI GAGTC 4 cut(s) 115, 273, 338, 436
Ppu21I YACGTR 1 cut(s) 362
PscI ACATGT 1 cut(s) 466
PshBI ATTAAT 1 cut(s) 474
PspCI CACGTG 1 cut(s) 362
PspN4I GGNNCC 1 cut(s) 444
RsaI GTAC 2 cut(s) 247, 367
RsaNI GTAC 2 cut(s) 246, 366
SalI GTCGAC 1 cut(s) 412
SaqAI TTAA 5 cut(s) 474, 548, 611, 681, 857
SatI GCNGC 3 cut(s) 126, 242, 887
Sau3AI GATC 2 cut(s) 55, 556
SchI GAGTC 4 cut(s) 116, 274, 338, 437
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
SpeI ACTAGT 1 cut(s) 248
SphI GCATGC 1 cut(s) 867
Sse9I AATT 9 cut(s) 61, 82, 173, 292, 301, 612, 641, 708, 850
SsiI CCGC 4 cut(s) 125, 242, 521, 832
SspMI CTAG 4 cut(s) 249, 278, 585, 916
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 1 cut(s) 364
TaqI TCGA 4 cut(s) 120, 263, 342, 413
TasI AATT 9 cut(s) 61, 82, 173, 292, 301, 612, 641, 708, 850
TauI GCSGC 2 cut(s) 128, 244
TfiI GAWTC 2 cut(s) 461, 581
Tru1I TTAA 5 cut(s) 474, 548, 611, 681, 857
Tru9I TTAA 5 cut(s) 474, 548, 611, 681, 857
TscAI CASTG 3 cut(s) 40, 136, 334
TseFI GTSAC 1 cut(s) 12
TseI GCWGC 1 cut(s) 886
Tsp45I GTSAC 1 cut(s) 12
TspDTI ATGAA 4 cut(s) 417, 452, 605, 654
TspRI CASTG 3 cut(s) 40, 136, 334
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 4 cut(s) 61, 82, 292, 708
XceI RCATGY 4 cut(s) 237, 470, 659, 867
XmiI GTMKAC 1 cut(s) 413
XmnI GAANNNNTTC 1 cut(s) 784
XspI CTAG 4 cut(s) 249, 278, 585, 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.