pycom05g27540

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013321
Physical Location & Seq
Reverse (-)
28507942 .. 28509651
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g27540.1

Sequence Viewer

Length: 963 bp
ATGGATGCCGGGGCATGTGATTTGCAGGATAGGAGTGAGGTGTTGGGTGGAGTTGATCAAAAGGGGAGCGAAAAGTCGAAAGATTCTGTTGTTTGGTCGGTTGTTGAGACTGTCATTGTGATTGAATCCGAAGAGGATGTTCGTGTCAACGGGAAAAATGAAGGTTTGGACACATTGGGGAGTGAGCTGGGGTCTGATAAAGCACTGGTAGAAGGGCTTGAGAAGCCGAATGATAAGGAGGCGTGCGGACAGCGTAGTAGTGATGAGGTACCTATACAAGTTGAGCAAGGAGATGAGGTACCTAGTCAAGTTGAGCAAGGAGCTAATAAGAATTCAAACAATTTGGTTGATGGGAATGTGTCTGAGACTGTTGTTGTCATTAATTCGGGTGAGTCTGCGAGCGTAGTTGGAGATAATATAAGATTGGTTGCAAAAGTTGAGGAATTGGAGTCGAGTAAGGTATTAGCAGAAGATTCAAAGAAGAAGGTGCCTCAAGCAGAAAAGGATTCATGTGTGATTGATATGAAGTGTGGGAGTGGTAAAGGCTTTAGTGATAGATGGGATGGGGAAAGGGTTTGCAGGATTTGCCATTTAGGTTCTGATATCTCACCGGACAGAAAAATGGAAACTGCTAATAGTTCTATCACAGCGGATTTAATTAATCTCGGTTGTGGGTGTAAAGATGAGCTTGGCGTTGCTCATGGTCATTGTGCCGAAGCATGGTTTAGGCTAAGAGGAAACAGGACATGTGAAATATGTGGTGAGACTGCGCAAAATATTACCGGTCATGGGGATAACGGATTTATGGAGGAGTGGCATGAAGCAACGCTTGCTGATATCGGTAATAATTCATTGGAGAGGAATGTATCATGTTGGCGGCGACAGCCATTTTGCAACTTCTTAATGGCGTGTCTGGTAATAGCTTTTGTACTTCCATGGTTTTTTCGTGTGAATATGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

321

Amino Acids

34.81

Weight (kDa)

4.62

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 193 - 253 3.2e-06 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013321)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34200 AT2G34200
fragaria_vesca FvH4_3g07460
malus_domestica MD10G1277000.v1.1
prunus_persica Prupe.4G064900_v2.0.a1 Prupe.4G064900_v2.0.a1
pyrus_communis pycom05g27540 pycom10g23130
rosa_chinensis RchiOBHm_Chr5g0011391
rosa_laevigata RLG00000031829
rosa_multiflora Rmu_sc0009975.1_g000013
rosa_roxburghii Rroxscaffold_1G00065060
rosa_rugosa Rorug04G0456800
rosa_samantha Rh5AG088500 Rh5BG083600 Rh5CG096900 Rh5DG083500
rosa_wichuraiana Rw5G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 771
Acc65I GGTACC 2 cut(s) 268, 298
AccB1I GGYRCC 3 cut(s) 268, 298, 487
AciI CCGC 3 cut(s) 246, 650, 877
AcsI RAATTY 1 cut(s) 331
AfaI GTAC 3 cut(s) 270, 300, 930
AfiI CCNNNNNNNGG 2 cut(s) 720, 789
AflIII ACRYGT 1 cut(s) 746
AgeI ACCGGT 1 cut(s) 782
AgsI TTSAA 3 cut(s) 125, 336, 477
AluBI AGCT 4 cut(s) 187, 323, 688, 923
AluI AGCT 4 cut(s) 187, 323, 688, 923
Alw26I GTCTC 3 cut(s) 101, 359, 758
ApoI RAATTY 1 cut(s) 331
ArsI GACNNNNNNTTYG 2 cut(s) 873, 905
AseI ATTAAT 2 cut(s) 381, 660
AsiGI ACCGGT 1 cut(s) 782
Asp700I GAANNNNTTC 1 cut(s) 956
Asp718I GGTACC 2 cut(s) 268, 298
AspLEI GCGC 1 cut(s) 772
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 3 cut(s) 401, 600, 773
BanI GGYRCC 3 cut(s) 268, 298, 487
BccI CCATC 3 cut(s) 344, 552, 557
BclI TGATCA 1 cut(s) 55
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 3 cut(s) 101, 359, 758
BfaI CTAG 2 cut(s) 303, 961
BisI GCNGC 1 cut(s) 878
BlsI GCNGC 1 cut(s) 879
Bme1390I CCNGG 1 cut(s) 10
BmiI GGNNCC 3 cut(s) 270, 300, 489
BmrFI CCNGG 1 cut(s) 10
BpuEI CTTGAG 2 cut(s) 239, 477
BpuMI CCSGG 1 cut(s) 10
BsaJI CCNNGG 2 cut(s) 9, 935
BsaWI WCCGGW 2 cut(s) 610, 782
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 2 cut(s) 720, 789
Bse118I RCCGGY 1 cut(s) 782
Bse1I ACTGG 1 cut(s) 210
BseDI CCNNGG 2 cut(s) 9, 935
BseGI GGATG 3 cut(s) 10, 142, 568
BseLI CCNNNNNNNGG 2 cut(s) 720, 789
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 1 cut(s) 210
BseRI GAGGAG 1 cut(s) 824
BseYI CCCAGC 1 cut(s) 187
BshNI GGYRCC 3 cut(s) 268, 298, 487
BshTI ACCGGT 1 cut(s) 782
BsiSI CCGG 3 cut(s) 9, 611, 783
BslI CCNNNNNNNGG 2 cut(s) 720, 789
BsmAI GTCTC 3 cut(s) 101, 359, 758
Bsp143I GATC 1 cut(s) 55
Bsp19I CCATGG 1 cut(s) 935
BspACI CCGC 3 cut(s) 246, 650, 877
BspCNI CTCAG 1 cut(s) 355
BspLI GGNNCC 3 cut(s) 270, 300, 489
BspT107I GGYRCC 3 cut(s) 268, 298, 487
BsrFI RCCGGY 1 cut(s) 782
BsrI ACTGG 1 cut(s) 210
BssAI RCCGGY 1 cut(s) 782
BssECI CCNNGG 2 cut(s) 9, 935
BssMI GATC 1 cut(s) 55
BssT1I CCWWGG 1 cut(s) 935
Bst4CI ACNGT 2 cut(s) 112, 370
Bst6I CTCTTC 1 cut(s) 126
BstAPI GCANNNNNTGC 2 cut(s) 585, 830
BstC8I GCNNGC 3 cut(s) 244, 400, 831
BstDEI CTNAG 2 cut(s) 363, 731
BstDSI CCRYGG 1 cut(s) 935
BstF5I GGATG 3 cut(s) 10, 142, 568
BstHHI GCGC 1 cut(s) 772
BstKTI GATC 1 cut(s) 58
BstMAI GTCTC 3 cut(s) 101, 359, 758
BstMBI GATC 1 cut(s) 55
BstMWI GCNNNNNNNGC 4 cut(s) 223, 585, 830, 883
BstNSI RCATGY 2 cut(s) 18, 750
BstSCI CCNGG 1 cut(s) 8
BtgI CCRYGG 1 cut(s) 935
BtsCI GGATG 3 cut(s) 10, 142, 568
BtsIMutI CAGTG 1 cut(s) 203
Cac8I GCNNGC 3 cut(s) 244, 400, 831
CfoI GCGC 1 cut(s) 772
Cfr10I RCCGGY 1 cut(s) 782
Csp6I GTAC 3 cut(s) 269, 299, 929
CspAI ACCGGT 1 cut(s) 782
CviAII CATG 9 cut(s) 15, 510, 701, 720, 747, 788, 818, 870, 936
CviJI RGCY 9 cut(s) 187, 217, 226, 323, 546, 688, 730, 886, 923
CviKI_1 RGCY 9 cut(s) 187, 217, 226, 323, 546, 688, 730, 886, 923
CviQI GTAC 3 cut(s) 269, 299, 929
DdeI CTNAG 2 cut(s) 363, 731
DpnI GATC 1 cut(s) 57
DpnII GATC 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 935
Eco32I GATATC 2 cut(s) 604, 838
EcoRI GAATTC 1 cut(s) 331
EcoRV GATATC 2 cut(s) 604, 838
EcoT14I CCWWGG 1 cut(s) 935
ErhI CCWWGG 1 cut(s) 935
FaeI CATG 9 cut(s) 18, 513, 704, 723, 750, 791, 821, 873, 939
FalI AAGNNNNNCTT 4 cut(s) 672, 704, 813, 845
FatI CATG 9 cut(s) 14, 509, 700, 719, 746, 787, 817, 869, 935
FbaI TGATCA 1 cut(s) 55
Fnu4HI GCNGC 1 cut(s) 878
FokI GGATG 3 cut(s) 17, 149, 575
Fsp4HI GCNGC 1 cut(s) 878
FspBI CTAG 2 cut(s) 303, 961
FspI TGCGCA 1 cut(s) 771
GlaI GCGC 1 cut(s) 771
GluI GCNGC 1 cut(s) 878
GsaI CCCAGC 1 cut(s) 191
HapII CCGG 3 cut(s) 9, 611, 783
HhaI GCGC 1 cut(s) 772
Hin1II CATG 9 cut(s) 18, 513, 704, 723, 750, 791, 821, 873, 939
Hin6I GCGC 1 cut(s) 770
HinP1I GCGC 1 cut(s) 770
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 6 cut(s) 83, 125, 392, 449, 473, 506
HpaII CCGG 3 cut(s) 9, 611, 783
HphI GGTGA 3 cut(s) 401, 600, 773
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 4 cut(s) 130, 196, 364, 601
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 3 cut(s) 155, 206, 478
HpyCH4III ACNGT 2 cut(s) 112, 370
HpyCH4V TGCA 4 cut(s) 25, 431, 579, 894
HpyF10VI GCNNNNNNNGC 4 cut(s) 223, 585, 830, 883
HpyF3I CTNAG 2 cut(s) 363, 731
Hsp92II CATG 9 cut(s) 18, 513, 704, 723, 750, 791, 821, 873, 939
HspAI GCGC 1 cut(s) 770
KpnI GGTACC 2 cut(s) 272, 302
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 1 cut(s) 55
LmnI GCTCC 2 cut(s) 66, 320
LpnPI CCDG 9 cut(s) 11, 22, 173, 191, 565, 624, 727, 796, 899
MaeI CTAG 2 cut(s) 303, 961
MalI GATC 1 cut(s) 57
MboI GATC 1 cut(s) 55
MboII GAAGA 3 cut(s) 143, 482, 493
MluCI AATT 6 cut(s) 331, 340, 382, 443, 657, 847
MlyI GAGTC 2 cut(s) 401, 458
MmeI TCCRAC 1 cut(s) 388
MroXI GAANNNNTTC 1 cut(s) 956
MseI TTAA 4 cut(s) 381, 656, 660, 902
MspA1I CMGCKG 1 cut(s) 650
MspI CCGG 3 cut(s) 9, 611, 783
MspR9I CCNGG 1 cut(s) 10
MwoI GCNNNNNNNGC 4 cut(s) 223, 585, 830, 883
NciI CCSGG 1 cut(s) 10
NcoI CCATGG 1 cut(s) 935
NdeII GATC 1 cut(s) 55
NlaIII CATG 9 cut(s) 18, 513, 704, 723, 750, 791, 821, 873, 939
NlaIV GGNNCC 3 cut(s) 270, 300, 489
NsbI TGCGCA 1 cut(s) 771
NspI RCATGY 2 cut(s) 18, 750
PacI TTAATTAA 1 cut(s) 660
PciI ACATGT 1 cut(s) 746
PdmI GAANNNNTTC 1 cut(s) 956
PfeI GAWTC 4 cut(s) 83, 125, 473, 506
PinAI ACCGGT 1 cut(s) 782
PkrI GCNGC 1 cut(s) 879
PleI GAGTC 2 cut(s) 400, 457
PpsI GAGTC 2 cut(s) 400, 457
PscI ACATGT 1 cut(s) 746
PshBI ATTAAT 2 cut(s) 381, 660
PspFI CCCAGC 1 cut(s) 187
PspN4I GGNNCC 3 cut(s) 270, 300, 489
RsaI GTAC 3 cut(s) 270, 300, 930
RsaNI GTAC 3 cut(s) 269, 299, 929
SaqAI TTAA 4 cut(s) 381, 656, 660, 902
SatI GCNGC 1 cut(s) 878
Sau3AI GATC 1 cut(s) 55
SchI GAGTC 2 cut(s) 401, 458
ScrFI CCNGG 1 cut(s) 10
SmlI CTYRAG 2 cut(s) 218, 492
SmoI CTYRAG 2 cut(s) 218, 492
Sse9I AATT 6 cut(s) 331, 340, 382, 443, 657, 847
SsiI CCGC 3 cut(s) 246, 650, 877
SspI AATATT 1 cut(s) 778
SspMI CTAG 2 cut(s) 303, 961
StyD4I CCNGG 1 cut(s) 8
StyI CCWWGG 1 cut(s) 935
TaaI ACNGT 2 cut(s) 112, 370
TaqI TCGA 2 cut(s) 77, 452
TasI AATT 6 cut(s) 331, 340, 382, 443, 657, 847
TatI WGTACW 1 cut(s) 928
TauI GCSGC 1 cut(s) 880
TfiI GAWTC 4 cut(s) 83, 125, 473, 506
Tru1I TTAA 4 cut(s) 381, 656, 660, 902
Tru9I TTAA 4 cut(s) 381, 656, 660, 902
TscAI CASTG 1 cut(s) 210
TspDTI ATGAA 5 cut(s) 174, 498, 539, 834, 840
TspGWI ACGGA 1 cut(s) 813
TspRI CASTG 1 cut(s) 210
VspI ATTAAT 2 cut(s) 381, 660
XapI RAATTY 1 cut(s) 331
XceI RCATGY 2 cut(s) 18, 750
XmnI GAANNNNTTC 1 cut(s) 956
XspI CTAG 2 cut(s) 303, 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.