FvH4_3g07830

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011538
Physical Location & Seq
Reverse (-)
4606605 .. 4608188
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g07830.t2

Sequence Viewer

Length: 513 bp
ATGACGATAGTAGAGATGCAAGTGCACATGGACTGTTCTGGCTGTGAGAACAAGATAAAGAAAGCTCTCAAAAAGATTAAAGGAGTAGACGATATTGATGTGGATATAAACATGCAGAAGGTGACTGTCATGGGATGGGCTAAGCAAGAGAAGGTTTTAAAGGCAGTAAGGAGGACTGGAAGGACAGCTGAGCTTTGGCCTTACCCTTACAACCCTGAATATGGACACAACCTTAATGTTCAGTACTACCAGCAGCATCAGCCCCGTGATGGCCACGAACATCACCACCACCACCACAACCATCATGATCGTGGTGAGCATCGGAGTAAACACAACATCACCACCTACAATTCAATTCCGAATTACTCGTCCTATTCGTACAATTATTATAAGCACGGCTACAGTGGCATGGAGCACGGCTACTATCAGCCTCCTCCTTACTCCACCATGTTTAGTGAGCAGGACACTGCTGTATTCAGTGATGACAACCCTAATTCTTGTTCCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

171

Amino Acids

20.0

Weight (kDa)

6.97

Isoelectric Point (pI)

26.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 3 - 56 2.3e-15 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 390
AccI GTMKAC 1 cut(s) 87
AcoI YGGCCR 1 cut(s) 271
AfaI GTAC 2 cut(s) 245, 380
AfiI CCNNNNNNNGG 2 cut(s) 221, 269
AgsI TTSAA 1 cut(s) 354
AluBI AGCT 3 cut(s) 65, 188, 193
AluI AGCT 3 cut(s) 65, 188, 193
Alw21I GWGCWC 2 cut(s) 27, 417
Alw44I GTGCAC 1 cut(s) 23
AoxI GGCC 2 cut(s) 197, 271
ApaLI GTGCAC 1 cut(s) 23
ApeKI GCWGC 1 cut(s) 253
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AsuHPI GGTGA 4 cut(s) 133, 275, 326, 331
BaeGI GKGCMC 1 cut(s) 27
BalI TGGCCA 1 cut(s) 273
Bbv12I GWGCWC 2 cut(s) 27, 417
BbvI GCAGC 1 cut(s) 265
BccI CCATC 3 cut(s) 129, 263, 309
BceAI ACGGC 2 cut(s) 412, 433
BfmI CTRYAG 1 cut(s) 400
BisI GCNGC 1 cut(s) 254
BlpI GCTNAGC 2 cut(s) 141, 189
BlsI GCNGC 1 cut(s) 255
BmcAI AGTACT 1 cut(s) 245
BmsI GCATC 3 cut(s) 6, 265, 328
Bpu1102I GCTNAGC 2 cut(s) 141, 189
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 269
Bse1I ACTGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 221, 269
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 1 cut(s) 423
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 1 cut(s) 265
BshFI GGCC 2 cut(s) 199, 273
BsiHKAI GWGCWC 2 cut(s) 27, 417
BslI CCNNNNNNNGG 2 cut(s) 221, 269
BsnI GGCC 2 cut(s) 199, 273
Bsp1286I GDGCHC 2 cut(s) 27, 417
Bsp143I GATC 1 cut(s) 307
Bsp1720I GCTNAGC 2 cut(s) 141, 189
BspANI GGCC 2 cut(s) 199, 273
BspCNI CTCAG 1 cut(s) 181
BspHI TCATGA 1 cut(s) 304
BsrI ACTGG 1 cut(s) 181
BssMI GATC 1 cut(s) 307
Bst4CI ACNGT 3 cut(s) 35, 127, 404
BstDEI CTNAG 2 cut(s) 141, 189
BstF5I GGATG 1 cut(s) 140
BstKTI GATC 1 cut(s) 310
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 2 cut(s) 259, 405
BstNSI RCATGY 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 400
BstSLI GKGCMC 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 265
BsuRI GGCC 2 cut(s) 199, 273
BtsCI GGATG 1 cut(s) 140
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 3 cut(s) 409, 465, 484
CciI TCATGA 1 cut(s) 304
Csp6I GTAC 2 cut(s) 244, 379
CviAII CATG 6 cut(s) 28, 112, 130, 305, 409, 448
CviQI GTAC 2 cut(s) 244, 379
DdeI CTNAG 2 cut(s) 141, 189
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
DraI TTTAAA 1 cut(s) 159
EaeI YGGCCR 1 cut(s) 271
FaeI CATG 6 cut(s) 31, 115, 133, 308, 412, 451
FatI CATG 6 cut(s) 27, 111, 129, 304, 408, 447
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 1 cut(s) 254
FokI GGATG 1 cut(s) 147
Fsp4HI GCNGC 1 cut(s) 254
GluI GCNGC 1 cut(s) 254
HaeIII GGCC 2 cut(s) 199, 273
Hin1II CATG 6 cut(s) 31, 115, 133, 308, 412, 451
HphI GGTGA 4 cut(s) 133, 275, 326, 331
Hpy166II GTNNAC 3 cut(s) 25, 88, 329
Hpy188I TCNGA 2 cut(s) 324, 360
Hpy188III TCNNGA 1 cut(s) 305
Hpy8I GTNNAC 3 cut(s) 25, 88, 329
HpyAV CCTTC 3 cut(s) 112, 145, 174
HpyCH4III ACNGT 3 cut(s) 35, 127, 404
HpyCH4V TGCA 3 cut(s) 19, 25, 115
HpyF10VI GCNNNNNNNGC 2 cut(s) 259, 405
HpyF3I CTNAG 2 cut(s) 141, 189
Hsp92II CATG 6 cut(s) 31, 115, 133, 308, 412, 451
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 412
LpnPI CCDG 5 cut(s) 24, 162, 228, 263, 446
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 3 cut(s) 6, 265, 328
MaeIII GTNAC 1 cut(s) 121
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MhlI GDGCHC 2 cut(s) 27, 417
MlsI TGGCCA 1 cut(s) 273
MluCI AATT 5 cut(s) 349, 354, 361, 382, 493
MluNI TGGCCA 1 cut(s) 273
MnlI CCTC 3 cut(s) 165, 441, 444
Mox20I TGGCCA 1 cut(s) 273
MscI TGGCCA 1 cut(s) 273
MseI TTAA 3 cut(s) 78, 158, 234
MslI CAYNNNNRTG 1 cut(s) 309
Msp20I TGGCCA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 259, 405
NdeII GATC 1 cut(s) 307
NlaIII CATG 6 cut(s) 31, 115, 133, 308, 412, 451
NmuCI GTSAC 1 cut(s) 121
NspI RCATGY 1 cut(s) 115
PagI TCATGA 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 374
PkrI GCNGC 1 cut(s) 255
PsiI TTATAA 1 cut(s) 390
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 245, 380
RsaNI GTAC 2 cut(s) 244, 379
RseI CAYNNNNRTG 1 cut(s) 309
SaqAI TTAA 3 cut(s) 78, 158, 234
SatI GCNGC 1 cut(s) 254
Sau3AI GATC 1 cut(s) 307
ScaI AGTACT 1 cut(s) 245
SduI GDGCHC 2 cut(s) 27, 417
SetI ASST 7 cut(s) 67, 123, 156, 190, 195, 234, 347
SfaNI GCATC 3 cut(s) 6, 265, 328
SfcI CTRYAG 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 309
Sse9I AATT 5 cut(s) 349, 354, 361, 382, 493
TaaI ACNGT 3 cut(s) 35, 127, 404
TasI AATT 5 cut(s) 349, 354, 361, 382, 493
TatI WGTACW 1 cut(s) 243
Tru1I TTAA 3 cut(s) 78, 158, 234
Tru9I TTAA 3 cut(s) 78, 158, 234
TscAI CASTG 3 cut(s) 409, 472, 484
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 121
TspRI CASTG 3 cut(s) 409, 472, 484
VneI GTGCAC 1 cut(s) 23
XceI RCATGY 1 cut(s) 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 87
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.