RLG00000031775

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011538
Physical Location & Seq
Forward (+)
8155800 .. 8156903
1104 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031775

Sequence Viewer

Length: 495 bp
ATGACGATTGTAGAGATGCAAGTGCACATGGACTGTTCTGGCTGTGAGAACAAGATAAAGAAAGCTCTCAAAAAGCTTAAAGGAGTAGACGATATTGACGTGGATATAAACATGCAGAAGGTGACTGTCATGGGATGGGCTAAACAAGAGAAGGTTTTAAAGGCAGTGAGGAGGACTGGAAGGACAGCTGAGCTTTGGCCTTACCCTTACAACCCTGAATATGGCCACAACCTTAATGTTCAGTACTACCAGCAGCATCAGCCCCGCGATGGCCACAAACATCACCACCACCATCATGATCATCAGAGTAAACACAAGATCACCACCTACAATTCGATTCCCAATTACTCGTCTTATTCGTACAATTACTACAAGCATGGCTACAGTGGCAACGAGCATGGCTATTATCAGCCTCCTCCTTACTCCACCATGTTTAATGAGCAGGACACTGCTGTGTTCAGTGATGAAAATCCTCATGCTTGTTCCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

165

Amino Acids

19.28

Weight (kDa)

7.8

Isoelectric Point (pI)

25.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 8 - 61 7.7e-16 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 87
AccII CGCG 1 cut(s) 267
AciI CCGC 1 cut(s) 265
AcoI YGGCCR 2 cut(s) 223, 271
AfaI GTAC 2 cut(s) 245, 362
AfiI CCNNNNNNNGG 2 cut(s) 221, 269
AjiI CACGTC 1 cut(s) 100
AleI CACNNNNGTG 1 cut(s) 452
AluBI AGCT 4 cut(s) 65, 76, 188, 193
AluI AGCT 4 cut(s) 65, 76, 188, 193
Alw21I GWGCWC 1 cut(s) 27
Alw44I GTGCAC 1 cut(s) 23
AoxI GGCC 3 cut(s) 197, 223, 271
ApaLI GTGCAC 1 cut(s) 23
ApeKI GCWGC 1 cut(s) 253
AsuHPI GGTGA 3 cut(s) 133, 275, 313
BaeGI GKGCMC 1 cut(s) 27
BalI TGGCCA 2 cut(s) 225, 273
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 265
BccI CCATC 3 cut(s) 129, 263, 300
BclI TGATCA 1 cut(s) 298
BfmI CTRYAG 1 cut(s) 382
BisI GCNGC 1 cut(s) 254
BlpI GCTNAGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 255
BmcAI AGTACT 1 cut(s) 245
BmgBI CACGTC 1 cut(s) 100
BmsI GCATC 2 cut(s) 6, 265
Bpu1102I GCTNAGC 1 cut(s) 189
BsaBI GATNNNNATC 1 cut(s) 468
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 269
Bse1I ACTGG 1 cut(s) 181
Bse8I GATNNNNATC 1 cut(s) 468
BseGI GGATG 1 cut(s) 140
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 2 cut(s) 221, 269
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 2 cut(s) 184, 405
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 1 cut(s) 265
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 3 cut(s) 199, 225, 273
BsiHKAI GWGCWC 1 cut(s) 27
BslI CCNNNNNNNGG 2 cut(s) 221, 269
BsnI GGCC 3 cut(s) 199, 225, 273
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 2 cut(s) 298, 318
Bsp1720I GCTNAGC 1 cut(s) 189
BspACI CCGC 1 cut(s) 265
BspANI GGCC 3 cut(s) 199, 225, 273
BspCNI CTCAG 1 cut(s) 181
BspFNI CGCG 1 cut(s) 267
BspHI TCATGA 1 cut(s) 295
BsrI ACTGG 1 cut(s) 181
BssMI GATC 2 cut(s) 298, 318
Bst4CI ACNGT 3 cut(s) 35, 127, 386
BstDEI CTNAG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 140
BstFNI CGCG 1 cut(s) 267
BstKTI GATC 2 cut(s) 301, 321
BstMBI GATC 2 cut(s) 298, 318
BstMWI GCNNNNNNNGC 2 cut(s) 259, 387
BstNSI RCATGY 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 382
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 265
BsuRI GGCC 3 cut(s) 199, 225, 273
BtgZI GCGATG 1 cut(s) 282
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 1 cut(s) 140
BtsI GCAGTG 2 cut(s) 171, 447
BtsIMutI CAGTG 4 cut(s) 171, 391, 447, 466
CciI TCATGA 1 cut(s) 295
Csp6I GTAC 2 cut(s) 244, 361
CviAII CATG 8 cut(s) 28, 112, 130, 296, 377, 398, 430, 476
CviQI GTAC 2 cut(s) 244, 361
DdeI CTNAG 1 cut(s) 189
DpnI GATC 2 cut(s) 300, 320
DpnII GATC 2 cut(s) 298, 318
DraI TTTAAA 1 cut(s) 159
EaeI YGGCCR 2 cut(s) 223, 271
FaeI CATG 8 cut(s) 31, 115, 133, 299, 380, 401, 433, 479
FatI CATG 8 cut(s) 27, 111, 129, 295, 376, 397, 429, 475
FauI CCCGC 1 cut(s) 272
FbaI TGATCA 1 cut(s) 298
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 1 cut(s) 254
FokI GGATG 1 cut(s) 147
Fsp4HI GCNGC 1 cut(s) 254
GluI GCNGC 1 cut(s) 254
HaeIII GGCC 3 cut(s) 199, 225, 273
Hin1II CATG 8 cut(s) 31, 115, 133, 299, 380, 401, 433, 479
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 1 cut(s) 337
HphI GGTGA 3 cut(s) 133, 275, 313
Hpy166II GTNNAC 3 cut(s) 25, 88, 311
Hpy188I TCNGA 1 cut(s) 306
Hpy188III TCNNGA 1 cut(s) 296
Hpy8I GTNNAC 3 cut(s) 25, 88, 311
HpyAV CCTTC 3 cut(s) 112, 145, 174
HpyCH4III ACNGT 3 cut(s) 35, 127, 386
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 3 cut(s) 19, 25, 115
HpyF10VI GCNNNNNNNGC 2 cut(s) 259, 387
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 8 cut(s) 31, 115, 133, 299, 380, 401, 433, 479
Ksp22I TGATCA 1 cut(s) 298
Kzo9I GATC 2 cut(s) 298, 318
LpnPI CCDG 5 cut(s) 24, 162, 228, 263, 428
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 2 cut(s) 6, 265
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 121
MalI GATC 2 cut(s) 300, 320
MboI GATC 2 cut(s) 298, 318
MhlI GDGCHC 1 cut(s) 27
MlsI TGGCCA 2 cut(s) 225, 273
MluCI AATT 3 cut(s) 331, 343, 364
MluNI TGGCCA 2 cut(s) 225, 273
MnlI CCTC 5 cut(s) 162, 165, 423, 426, 483
Mox20I TGGCCA 2 cut(s) 225, 273
MscI TGGCCA 2 cut(s) 225, 273
MseI TTAA 4 cut(s) 78, 158, 234, 435
MslI CAYNNNNRTG 2 cut(s) 294, 452
Msp20I TGGCCA 2 cut(s) 225, 273
MspA1I CMGCKG 1 cut(s) 188
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 2 cut(s) 259, 387
NdeII GATC 2 cut(s) 298, 318
NlaIII CATG 8 cut(s) 31, 115, 133, 299, 380, 401, 433, 479
NmuCI GTSAC 1 cut(s) 121
NspI RCATGY 1 cut(s) 115
OliI CACNNNNGTG 1 cut(s) 452
PagI TCATGA 1 cut(s) 295
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 356
PfeI GAWTC 1 cut(s) 337
PkrI GCNGC 1 cut(s) 255
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 245, 362
RsaNI GTAC 2 cut(s) 244, 361
RseI CAYNNNNRTG 2 cut(s) 294, 452
SaqAI TTAA 4 cut(s) 78, 158, 234, 435
SatI GCNGC 1 cut(s) 254
Sau3AI GATC 2 cut(s) 298, 318
ScaI AGTACT 1 cut(s) 245
SduI GDGCHC 1 cut(s) 27
SetI ASST 9 cut(s) 67, 78, 102, 123, 156, 190, 195, 234, 329
SfaNI GCATC 2 cut(s) 6, 265
SfcI CTRYAG 1 cut(s) 382
SmiMI CAYNNNNRTG 2 cut(s) 294, 452
Sse9I AATT 3 cut(s) 331, 343, 364
SsiI CCGC 1 cut(s) 265
TaaI ACNGT 3 cut(s) 35, 127, 386
TaiI ACGT 1 cut(s) 102
TaqI TCGA 1 cut(s) 335
TasI AATT 3 cut(s) 331, 343, 364
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 1 cut(s) 337
Tru1I TTAA 4 cut(s) 78, 158, 234, 435
Tru9I TTAA 4 cut(s) 78, 158, 234, 435
TscAI CASTG 4 cut(s) 171, 391, 454, 466
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 1 cut(s) 480
TspRI CASTG 4 cut(s) 171, 391, 454, 466
VneI GTGCAC 1 cut(s) 23
XceI RCATGY 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 87
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.