MD10G1280500.v1.1

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011538
Physical Location & Seq
Reverse (-)
37129338 .. 37132120
2783 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1280500.v1.1.491

Sequence Viewer

Length: 510 bp
ATGACGATCGTAGAGATGATTGTGACCATGGACTGTGCTGGCTGCGAGAACAAGATTAGGAGTGCTCTAAAAAAAATGAAAGGAGTAGATGCTGTTGACGTGGACTTCAACATGCAGAAGGTGACTGTAATGGGATGGGCAGATCAAGAGAAGGTTTTAAGGGCAGTAAGGAAGACTGGAAAGAGAGCTGAGCTTTGGCCTTACCCTTACAACCCTCAATACCACAGCATTGGTCTTGACTACTACCAGCAACATCAGCCGCTTGATCATCATCACAATCATCATCGTCATAAGCATCATCATGACCGTCGGATAACTGCCTACCAACTTCCCATCACTACCTACAAATCAGTACCTAAATCCTCCTCCTATTCATACAACTACTACAAGCATGGCTCCACTAATCAGGAACACGGCTACTATCAACCTCCTCCTTATTCCACCATGTTTAACGAGCAGGCCACTGCTGTGTTCAGTGATGAAAACCCTCATGCTTGTTCCATAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

170

Amino Acids

19.77

Weight (kDa)

8.65

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 6 - 61 8.3e-18 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 260
AfaI GTAC 1 cut(s) 354
AgsI TTSAA 1 cut(s) 109
AjiI CACGTC 1 cut(s) 100
AleI CACNNNNGTG 1 cut(s) 467
AluBI AGCT 2 cut(s) 188, 193
AluI AGCT 2 cut(s) 188, 193
Alw21I GWGCWC 1 cut(s) 67
AoxI GGCC 2 cut(s) 197, 459
ApeKI GCWGC 1 cut(s) 42
AsuHPI GGTGA 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 67
BbvI GCAGC 1 cut(s) 29
BccI CCATC 2 cut(s) 129, 341
BceAI ACGGC 1 cut(s) 430
BclI TGATCA 1 cut(s) 265
BisI GCNGC 2 cut(s) 43, 260
BlpI GCTNAGC 1 cut(s) 189
BlsI GCNGC 2 cut(s) 44, 261
BmgBI CACGTC 1 cut(s) 100
BmiI GGNNCC 1 cut(s) 397
BmsI GCATC 2 cut(s) 79, 304
BpiI GAAGAC 1 cut(s) 179
Bpu1102I GCTNAGC 1 cut(s) 189
BsaBI GATNNNNATC 1 cut(s) 270
BsaJI CCNNGG 1 cut(s) 27
Bse1I ACTGG 1 cut(s) 181
Bse8I GATNNNNATC 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 140
BseJI GATNNNNATC 1 cut(s) 270
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 2 cut(s) 355, 420
BseXI GCAGC 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 199, 461
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 67
BsnI GGCC 2 cut(s) 199, 461
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 3 cut(s) 6, 142, 265
Bsp1720I GCTNAGC 1 cut(s) 189
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 1 cut(s) 260
BspANI GGCC 2 cut(s) 199, 461
BspCNI CTCAG 1 cut(s) 181
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 397
BsrI ACTGG 1 cut(s) 181
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 3 cut(s) 6, 142, 265
BssT1I CCWWGG 1 cut(s) 27
Bst4CI ACNGT 3 cut(s) 35, 127, 308
BstC8I GCNNGC 2 cut(s) 40, 459
BstDEI CTNAG 1 cut(s) 189
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 140
BstKTI GATC 3 cut(s) 9, 145, 268
BstMBI GATC 3 cut(s) 6, 142, 265
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 1 cut(s) 256
BstNSI RCATGY 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 29
BstV2I GAAGAC 1 cut(s) 179
BstXI CCANNNNNNTGG 1 cut(s) 230
BsuRI GGCC 2 cut(s) 199, 461
BtgI CCRYGG 1 cut(s) 27
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 1 cut(s) 140
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 2 cut(s) 462, 481
Cac8I GCNNGC 2 cut(s) 40, 459
CciI TCATGA 1 cut(s) 301
Csp6I GTAC 1 cut(s) 353
CviAII CATG 6 cut(s) 28, 112, 302, 392, 445, 491
CviJI RGCY 8 cut(s) 42, 188, 193, 199, 259, 396, 417, 461
CviKI_1 RGCY 8 cut(s) 42, 188, 193, 199, 259, 396, 417, 461
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 1 cut(s) 189
DpnI GATC 3 cut(s) 8, 144, 267
DpnII GATC 3 cut(s) 6, 142, 265
Eco130I CCWWGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 6 cut(s) 31, 115, 305, 395, 448, 494
FaiI YATR 9 cut(s) 29, 113, 291, 303, 376, 393, 446, 492, 503
FatI CATG 6 cut(s) 27, 111, 301, 391, 444, 490
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 2 cut(s) 43, 260
FokI GGATG 1 cut(s) 147
Fsp4HI GCNGC 2 cut(s) 43, 260
GluI GCNGC 2 cut(s) 43, 260
HaeIII GGCC 2 cut(s) 199, 461
Hin1II CATG 6 cut(s) 31, 115, 305, 395, 448, 494
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 2 cut(s) 97, 103
Hpy188I TCNGA 1 cut(s) 312
Hpy188III TCNNGA 4 cut(s) 146, 236, 302, 407
Hpy8I GTNNAC 2 cut(s) 97, 103
Hpy99I CGWCG 1 cut(s) 312
HpyAV CCTTC 2 cut(s) 112, 145
HpyCH4III ACNGT 3 cut(s) 35, 127, 308
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 1 cut(s) 256
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 6 cut(s) 31, 115, 305, 395, 448, 494
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 3 cut(s) 6, 142, 265
LmnI GCTCC 1 cut(s) 401
LpnPI CCDG 5 cut(s) 24, 162, 260, 392, 443
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 2 cut(s) 79, 304
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 22, 121
MalI GATC 3 cut(s) 8, 144, 267
MboI GATC 3 cut(s) 6, 142, 265
MboII GAAGA 1 cut(s) 184
MhlI GDGCHC 1 cut(s) 67
MmeI TCCRAC 1 cut(s) 290
MnlI CCTC 6 cut(s) 225, 373, 376, 438, 441, 498
MseI TTAA 2 cut(s) 158, 450
MslI CAYNNNNRTG 2 cut(s) 300, 467
MwoI GCNNNNNNNGC 1 cut(s) 256
NcoI CCATGG 1 cut(s) 27
NdeII GATC 3 cut(s) 6, 142, 265
NlaIII CATG 6 cut(s) 31, 115, 305, 395, 448, 494
NlaIV GGNNCC 1 cut(s) 397
NmuCI GTSAC 2 cut(s) 22, 121
NspI RCATGY 1 cut(s) 115
OliI CACNNNNGTG 1 cut(s) 467
PagI TCATGA 1 cut(s) 301
PkrI GCNGC 2 cut(s) 44, 261
Ple19I CGATCG 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 397
PvuI CGATCG 1 cut(s) 9
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
RseI CAYNNNNRTG 2 cut(s) 300, 467
SaqAI TTAA 2 cut(s) 158, 450
SatI GCNGC 2 cut(s) 43, 260
Sau3AI GATC 3 cut(s) 6, 142, 265
SduI GDGCHC 1 cut(s) 67
SetI ASST 8 cut(s) 102, 123, 156, 190, 195, 344, 358, 430
SfaNI GCATC 2 cut(s) 79, 304
SmiMI CAYNNNNRTG 2 cut(s) 300, 467
SsiI CCGC 1 cut(s) 260
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 3 cut(s) 35, 127, 308
TaiI ACGT 1 cut(s) 102
TauI GCSGC 1 cut(s) 262
Tru1I TTAA 2 cut(s) 158, 450
Tru9I TTAA 2 cut(s) 158, 450
TscAI CASTG 2 cut(s) 469, 481
TseFI GTSAC 2 cut(s) 22, 121
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 2 cut(s) 22, 121
TspDTI ATGAA 3 cut(s) 92, 363, 495
TspRI CASTG 2 cut(s) 469, 481
XceI RCATGY 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.