Rh5BG078900

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011538
Physical Location & Seq
Forward (+)
6668444 .. 6670237
1794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG078900.1

Sequence Viewer

Length: 504 bp
ATGACGATTGTAGAGATGCAAGTGCACATGGACTGTTCTGGCTGTGAGAACAAGATAAAGAAAGCTCTCAAAAAGCTTAAAGGAGTAGACGATATTGACGTGGATATAAACATGCAGAAGGTGACTGTCATGGGATGGGCTAAACAAGAGAAGGTTTTAAAGGCAGTGAGGAGGACTGGAAGGACAGCTGAGCTTTGGCCTTACCCTTACAACCCTGAATATGGCCACAACCTTAATCTTCAGTACTACCAGCAGCATCAGCCCCGCGATGGCCACGAAAATCACCACCACCACCACCACCATCATGATCATCAAAGTAAACACAAGATCACCACCTACAATTCGATTCCCAATTACTCGTCTTATTCGTACAATTACTACAAGCATGGCTACAGTGGCAAGGAGCATGGCTATTATCAGCCTCCTCCTTACTCCACCATGTTTAATGAGCAGGACACTGCTGTGTTCAGTGATGAAAACCCTCATGCCTGTTCCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

167

Amino Acids

19.7

Weight (kDa)

7.3

Isoelectric Point (pI)

25.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 8 - 61 8e-16 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 87
AccII CGCG 1 cut(s) 267
AciI CCGC 1 cut(s) 265
AcoI YGGCCR 2 cut(s) 223, 271
AcuI CTGAAG 1 cut(s) 224
AfaI GTAC 2 cut(s) 245, 371
AfiI CCNNNNNNNGG 2 cut(s) 221, 269
AjiI CACGTC 1 cut(s) 100
AleI CACNNNNGTG 1 cut(s) 461
AluBI AGCT 4 cut(s) 65, 76, 188, 193
AluI AGCT 4 cut(s) 65, 76, 188, 193
Alw21I GWGCWC 1 cut(s) 27
Alw44I GTGCAC 1 cut(s) 23
AoxI GGCC 3 cut(s) 197, 223, 271
ApaLI GTGCAC 1 cut(s) 23
ApeKI GCWGC 1 cut(s) 253
AsuHPI GGTGA 3 cut(s) 133, 275, 322
BaeGI GKGCMC 1 cut(s) 27
BalI TGGCCA 2 cut(s) 225, 273
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 265
BccI CCATC 3 cut(s) 129, 263, 309
BclI TGATCA 1 cut(s) 307
BfmI CTRYAG 1 cut(s) 391
BisI GCNGC 1 cut(s) 254
BlpI GCTNAGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 255
BmcAI AGTACT 1 cut(s) 245
BmgBI CACGTC 1 cut(s) 100
BmsI GCATC 2 cut(s) 6, 265
Bpu1102I GCTNAGC 1 cut(s) 189
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 269
Bse1I ACTGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 221, 269
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 2 cut(s) 184, 414
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 1 cut(s) 265
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 3 cut(s) 199, 225, 273
BsiHKAI GWGCWC 1 cut(s) 27
BslI CCNNNNNNNGG 2 cut(s) 221, 269
BsnI GGCC 3 cut(s) 199, 225, 273
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 2 cut(s) 307, 327
Bsp1720I GCTNAGC 1 cut(s) 189
BspACI CCGC 1 cut(s) 265
BspANI GGCC 3 cut(s) 199, 225, 273
BspCNI CTCAG 1 cut(s) 181
BspFNI CGCG 1 cut(s) 267
BspHI TCATGA 1 cut(s) 304
BsrI ACTGG 1 cut(s) 181
BssMI GATC 2 cut(s) 307, 327
Bst4CI ACNGT 3 cut(s) 35, 127, 395
BstDEI CTNAG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 140
BstFNI CGCG 1 cut(s) 267
BstKTI GATC 2 cut(s) 310, 330
BstMBI GATC 2 cut(s) 307, 327
BstMWI GCNNNNNNNGC 2 cut(s) 259, 396
BstNSI RCATGY 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 391
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 265
BsuRI GGCC 3 cut(s) 199, 225, 273
BtgZI GCGATG 1 cut(s) 282
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 1 cut(s) 140
BtsI GCAGTG 2 cut(s) 171, 456
BtsIMutI CAGTG 4 cut(s) 171, 400, 456, 475
CciI TCATGA 1 cut(s) 304
Csp6I GTAC 2 cut(s) 244, 370
CviAII CATG 8 cut(s) 28, 112, 130, 305, 386, 407, 439, 485
CviQI GTAC 2 cut(s) 244, 370
DdeI CTNAG 1 cut(s) 189
DpnI GATC 2 cut(s) 309, 329
DpnII GATC 2 cut(s) 307, 327
DraI TTTAAA 1 cut(s) 159
EaeI YGGCCR 2 cut(s) 223, 271
Eco57I CTGAAG 1 cut(s) 224
FaeI CATG 8 cut(s) 31, 115, 133, 308, 389, 410, 442, 488
FatI CATG 8 cut(s) 27, 111, 129, 304, 385, 406, 438, 484
FauI CCCGC 1 cut(s) 272
FbaI TGATCA 1 cut(s) 307
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 1 cut(s) 254
FokI GGATG 1 cut(s) 147
Fsp4HI GCNGC 1 cut(s) 254
GluI GCNGC 1 cut(s) 254
HaeIII GGCC 3 cut(s) 199, 225, 273
Hin1II CATG 8 cut(s) 31, 115, 133, 308, 389, 410, 442, 488
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 1 cut(s) 346
HphI GGTGA 3 cut(s) 133, 275, 322
Hpy166II GTNNAC 3 cut(s) 25, 88, 320
Hpy188III TCNNGA 1 cut(s) 305
Hpy8I GTNNAC 3 cut(s) 25, 88, 320
HpyAV CCTTC 3 cut(s) 112, 145, 174
HpyCH4III ACNGT 3 cut(s) 35, 127, 395
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 3 cut(s) 19, 25, 115
HpyF10VI GCNNNNNNNGC 2 cut(s) 259, 396
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 8 cut(s) 31, 115, 133, 308, 389, 410, 442, 488
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 2 cut(s) 307, 327
LmnI GCTCC 1 cut(s) 403
LpnPI CCDG 5 cut(s) 24, 162, 228, 263, 437
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 2 cut(s) 6, 265
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 121
MalI GATC 2 cut(s) 309, 329
MboI GATC 2 cut(s) 307, 327
MboII GAAGA 1 cut(s) 230
MhlI GDGCHC 1 cut(s) 27
MlsI TGGCCA 2 cut(s) 225, 273
MluCI AATT 3 cut(s) 340, 352, 373
MluNI TGGCCA 2 cut(s) 225, 273
MnlI CCTC 5 cut(s) 162, 165, 432, 435, 492
Mox20I TGGCCA 2 cut(s) 225, 273
MscI TGGCCA 2 cut(s) 225, 273
MseI TTAA 4 cut(s) 78, 158, 234, 444
MslI CAYNNNNRTG 2 cut(s) 303, 461
Msp20I TGGCCA 2 cut(s) 225, 273
MspA1I CMGCKG 1 cut(s) 188
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 2 cut(s) 259, 396
NdeII GATC 2 cut(s) 307, 327
NlaIII CATG 8 cut(s) 31, 115, 133, 308, 389, 410, 442, 488
NmuCI GTSAC 1 cut(s) 121
NspI RCATGY 1 cut(s) 115
OliI CACNNNNGTG 1 cut(s) 461
PagI TCATGA 1 cut(s) 304
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 365
PfeI GAWTC 1 cut(s) 346
PkrI GCNGC 1 cut(s) 255
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 245, 371
RsaNI GTAC 2 cut(s) 244, 370
RseI CAYNNNNRTG 2 cut(s) 303, 461
SaqAI TTAA 4 cut(s) 78, 158, 234, 444
SatI GCNGC 1 cut(s) 254
Sau3AI GATC 2 cut(s) 307, 327
ScaI AGTACT 1 cut(s) 245
SduI GDGCHC 1 cut(s) 27
SetI ASST 9 cut(s) 67, 78, 102, 123, 156, 190, 195, 234, 338
SfaNI GCATC 2 cut(s) 6, 265
SfcI CTRYAG 1 cut(s) 391
SmiMI CAYNNNNRTG 2 cut(s) 303, 461
Sse9I AATT 3 cut(s) 340, 352, 373
SsiI CCGC 1 cut(s) 265
TaaI ACNGT 3 cut(s) 35, 127, 395
TaiI ACGT 1 cut(s) 102
TaqI TCGA 1 cut(s) 344
TasI AATT 3 cut(s) 340, 352, 373
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 1 cut(s) 346
Tru1I TTAA 4 cut(s) 78, 158, 234, 444
Tru9I TTAA 4 cut(s) 78, 158, 234, 444
TscAI CASTG 4 cut(s) 171, 400, 463, 475
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 1 cut(s) 489
TspRI CASTG 4 cut(s) 171, 400, 463, 475
VneI GTGCAC 1 cut(s) 23
XceI RCATGY 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 87
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.