FvH4_3g08720

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012592
Physical Location & Seq
Forward (+)
5096098 .. 5097753
1656 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g08720.t1

Sequence Viewer

Length: 1656 bp
ATGGAAGTCCATGCCCACTTAATCAGACTAGGGTTGTCCCAAGACTTGAGCTTTCGGAACCATTTGATCAATCTCTATGCAAAAAGCAGGTTTTTTGGGCATGCCCGGAAGCTGGTCGACGAAAGTCCTGAACCAGATTTGGTTGCTTGGTCTGCTTTGGTTTCTGGGTATGCCCAGAATGGGCTTGGTAGAGAAGCCATTTCGGCTTTTCGTGAGATGTTTGGATTGGGGGTTAAGTGTAATGAGTTCACATTCCCAAGTGTGCTCAAGGCTTGCTCTAGTTGTAAAGATTTGAGACTGGGAATGCAGGTTCATGGGGTGGTTTTTGTGACCGGGTTTGAGTCCGACGAGTTTGTTGCTAATACTTTGGTTGTTATGTATTCTAAGTGTGGGGAGTTTGGGGATTCCAGGAGGTTGTTTGATGCCATTCCGGAGAGGAATGTTGTGTCGTGGAATGCGTTGTTTTCGTGTTATGTGCAGTGTGATTTCTTTGGGGAGGCTATGGAGTTGTTTGAGGAAATGGTGCTGAGTGGAATAAGGCCTAATGAGTATAGTCTGTCTAGTATAATAAATGTTGGTACCGGGTTGGGGGATGGTAGAAGAGGAAGGAAAATTCAAGGATATATAGTGAAGCTTGGATATGATTCGGATTTGTTCTCAGCCAATGCGCTTGTTGCCATGCATGCAAAAGCCATATGTCTTGAAGATGCAGTTTCAGTTTTTGAGAAAATTGCACAACCGGATATTGTATCCTGGAATGCTGTTATTGCTGGTTGTATTCTTCACAACTTCCATGGTCGGGCACTCAAATTGTTCAGGCAACTGAGTGGGTCAGGAATCCGTCCTAATATGTTTACAATATCAAGTGCACTTAAAGCTTGTGCTGGGATGGGTTTCAAGAAATTGGGTAGGCAGTTGCACTCTTTCTTGATAAAGATGGATACAGAATCAGATTCTTATGTCAAAGTTGGACTGATAGATATGTATTGCAAGTGTGAGATCATGACTGAGGCAAGGGTGCTTTTGAATATGATGCCAATGAAAGACATGATTGCATGCAATGCTGTTATCTCTGGTCATTCACAGATGGCCGAAGACATAGAAGCTGTAAGCCTCTTTCCTGAAATGCATAGGGAAGGAATAGGATTCAATGAGACTACTTTATCCACAGTCCTCAAATCCACCGCGAGCTTGCAGGCAGCAAAAGTTTGTGAACAAGTTCATTCTCTTTCTGTCAAAACAGATTTTCAGTCTGACAGGTATGTTATCAACAGCCTTCTTGATACGTACGGAAAATGTGGCCAAGTAGAAAATGCTGTAAGAATATTTGAAGAATGCAAATCTGAAGATCTGGTGGCTTTCACATCAATGATCACTGCCTATGCTCAGTATGAACAAGGCGAAGAAGCTCTAAAACTCTATGTGCAAATGCTACAGAGGGGGAACGAGCCTGATTCATTTGTATGCAGTTCTCTTCTGAATGCTTGTGCAAATTTGTCAGCGTATGAGCAAGGGAAGCAGATTCATGTTCATATTCTCAAGTTTGGATTTTTGTCAGATGCTTTTGCTGGAAATTCCCTTGTTAACATGTATTCAAAATGTGGAAGCATAGATGATGCAGGTCGTGCTTTCTCCGAGATACTGAGAGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

552

Amino Acids

61.01

Weight (kDa)

5.69

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 45 - 93 1.1e-08 PPR repeat family
PPR PF01535 48 - 78 3.6e-06 PPR repeat
PPR_2 PF13041 146 - 191 8.6e-11 PPR repeat family
PPR PF01535 149 - 179 2.6e-07 PPR repeat
PPR_2 PF13041 247 - 295 2.9e-08 PPR repeat family
PPR_2 PF13041 450 - 498 8.6e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 78, 298, 1610
Acc65I GGTACC 1 cut(s) 578
AccB1I GGYRCC 1 cut(s) 578
AccI GTMKAC 1 cut(s) 117
AccII CGCG 1 cut(s) 1187
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 1 cut(s) 1185
AcoI YGGCCR 2 cut(s) 1089, 1300
AcsI RAATTY 3 cut(s) 612, 1492, 1573
AcuI CTGAAG 1 cut(s) 1365
AfaI GTAC 2 cut(s) 580, 1289
AfiI CCNNNNNNNGG 4 cut(s) 112, 180, 588, 799
AflIII ACRYGT 1 cut(s) 1587
AgsI TTSAA 7 cut(s) 617, 704, 898, 1027, 1150, 1331, 1596
AjnI CCWGG 2 cut(s) 407, 752
AloI GAACNNNNNNTCC 2 cut(s) 294, 326
AluBI AGCT 7 cut(s) 51, 112, 634, 878, 1106, 1191, 1409
AluI AGCT 7 cut(s) 51, 112, 634, 878, 1106, 1191, 1409
Alw21I GWGCWC 2 cut(s) 267, 871
Alw26I GTCTC 2 cut(s) 289, 1148
Alw44I GTGCAC 1 cut(s) 867
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 3 cut(s) 539, 1089, 1300
ApaLI GTGCAC 1 cut(s) 867
ApeKI GCWGC 1 cut(s) 1199
ApoI RAATTY 3 cut(s) 612, 1492, 1573
Asp700I GAANNNNTTC 1 cut(s) 1218
Asp718I GGTACC 1 cut(s) 578
AspLEI GCGC 1 cut(s) 670
AsuC2I CCSGG 3 cut(s) 106, 334, 583
BaeGI GKGCMC 2 cut(s) 805, 871
BalI TGGCCA 1 cut(s) 1302
BanI GGYRCC 1 cut(s) 578
BbsI GAAGAC 1 cut(s) 1101
Bbv12I GWGCWC 2 cut(s) 267, 871
BbvI GCAGC 1 cut(s) 1211
BccI CCATC 4 cut(s) 587, 883, 931, 1081
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BciT130I CCWGG 2 cut(s) 409, 754
BciVI GTATCC 2 cut(s) 760, 934
BclI TGATCA 2 cut(s) 66, 1371
BcnI CCSGG 3 cut(s) 106, 334, 583
BcoDI GTCTC 2 cut(s) 289, 1148
BfaI CTAG 3 cut(s) 29, 279, 561
BfmI CTRYAG 1 cut(s) 1433
BfuAI ACCTGC 3 cut(s) 78, 298, 1610
BfuI GTATCC 2 cut(s) 760, 934
BglI GCCNNNNNGGC 1 cut(s) 203
BglII AGATCT 1 cut(s) 1348
BisI GCNGC 1 cut(s) 1200
BlsI GCNGC 1 cut(s) 1201
Bme1390I CCNGG 5 cut(s) 106, 334, 409, 583, 754
BmiI GGNNCC 2 cut(s) 59, 580
BmrFI CCNGG 5 cut(s) 106, 334, 409, 583, 754
BmrI ACTGGG 1 cut(s) 308
BmsI GCATC 5 cut(s) 412, 697, 1023, 1549, 1606
BmuI ACTGGG 1 cut(s) 308
BoxI GACNNNNGTC 1 cut(s) 123
BpiI GAAGAC 1 cut(s) 1101
BpuEI CTTGAG 3 cut(s) 67, 251, 1523
BpuMI CCSGG 3 cut(s) 106, 334, 583
BsaAI YACGTR 1 cut(s) 1287
BsaJI CCNNGG 1 cut(s) 793
BsaWI WCCGGW 2 cut(s) 430, 739
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 180, 588, 799
Bse1I ACTGG 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 1066
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 2 cut(s) 409, 754
BseDI CCNNGG 1 cut(s) 793
BseGI GGATG 2 cut(s) 598, 894
BseLI CCNNNNNNNGG 4 cut(s) 112, 180, 588, 799
BseMI GCAATG 1 cut(s) 1066
BseMII CTCAG 6 cut(s) 518, 672, 815, 999, 1400, 1634
BseNI ACTGG 1 cut(s) 303
BseSI GKGCMC 2 cut(s) 805, 871
BseXI GCAGC 1 cut(s) 1211
BseYI CCCAGC 1 cut(s) 884
BsgI GTGCAG 1 cut(s) 497
Bsh1236I CGCG 1 cut(s) 1187
BshFI GGCC 3 cut(s) 541, 1091, 1302
BshNI GGYRCC 1 cut(s) 578
BsiHKAI GWGCWC 2 cut(s) 267, 871
BsiSI CCGG 5 cut(s) 106, 333, 431, 582, 740
BsiWI CGTACG 1 cut(s) 1287
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 4 cut(s) 112, 180, 588, 799
BsmAI GTCTC 2 cut(s) 289, 1148
BsmFI GGGAC 1 cut(s) 22
BsmI GAATGC 5 cut(s) 309, 460, 763, 1340, 1486
BsnI GGCC 3 cut(s) 541, 1091, 1302
Bsp1286I GDGCHC 3 cut(s) 267, 805, 871
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 4 cut(s) 66, 999, 1348, 1371
Bsp19I CCATGG 1 cut(s) 793
BspACI CCGC 1 cut(s) 1185
BspANI GGCC 3 cut(s) 541, 1091, 1302
BspCNI CTCAG 6 cut(s) 519, 671, 816, 1000, 1399, 1635
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 1 cut(s) 1187
BspHI TCATGA 1 cut(s) 1002
BspLI GGNNCC 2 cut(s) 59, 580
BspMI ACCTGC 3 cut(s) 78, 298, 1610
BspT107I GGYRCC 1 cut(s) 578
BsrDI GCAATG 1 cut(s) 1066
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 793
BssMI GATC 4 cut(s) 66, 999, 1348, 1371
BssT1I CCWWGG 1 cut(s) 793
Bst2UI CCWGG 2 cut(s) 409, 754
Bst4CI ACNGT 1 cut(s) 1171
Bst6I CTCTTC 2 cut(s) 595, 1479
BstAPI GCANNNNNTGC 2 cut(s) 1061, 1625
BstBAI YACGTR 1 cut(s) 1287
BstC8I GCNNGC 7 cut(s) 102, 274, 684, 1057, 1189, 1193, 1197
BstDEI CTNAG 7 cut(s) 384, 527, 658, 824, 1008, 1386, 1643
BstDSI CCRYGG 1 cut(s) 793
BstF5I GGATG 2 cut(s) 598, 894
BstFNI CGCG 1 cut(s) 1187
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 4 cut(s) 69, 1002, 1351, 1374
BstMAI GTCTC 2 cut(s) 289, 1148
BstMBI GATC 4 cut(s) 66, 999, 1348, 1371
BstMWI GCNNNNNNNGC 9 cut(s) 152, 203, 674, 683, 767, 875, 1061, 1516, 1625
BstNI CCWGG 2 cut(s) 409, 754
BstNSI RCATGY 4 cut(s) 104, 686, 1059, 1591
BstPAI GACNNNNGTC 1 cut(s) 123
BstSCI CCNGG 5 cut(s) 104, 332, 407, 581, 752
BstSFI CTRYAG 1 cut(s) 1433
BstSLI GKGCMC 2 cut(s) 805, 871
BstSNI TACGTA 1 cut(s) 1287
BstUI CGCG 1 cut(s) 1187
BstV1I GCAGC 1 cut(s) 1211
BstV2I GAAGAC 1 cut(s) 1101
BstX2I RGATCY 1 cut(s) 1348
BstYI RGATCY 1 cut(s) 1348
BsuI GTATCC 2 cut(s) 760, 934
BsuRI GGCC 3 cut(s) 541, 1091, 1302
BtgI CCRYGG 1 cut(s) 793
BtsCI GGATG 2 cut(s) 598, 894
BtsI GCAGTG 2 cut(s) 485, 1374
BtsIMutI CAGTG 2 cut(s) 485, 1374
BveI ACCTGC 3 cut(s) 78, 298, 1610
Cac8I GCNNGC 7 cut(s) 102, 274, 684, 1057, 1189, 1193, 1197
CciI TCATGA 1 cut(s) 1002
CfoI GCGC 1 cut(s) 670
Csp6I GTAC 2 cut(s) 579, 1288
CviQI GTAC 2 cut(s) 579, 1288
DdeI CTNAG 7 cut(s) 384, 527, 658, 824, 1008, 1386, 1643
DpnI GATC 4 cut(s) 68, 1001, 1350, 1373
DpnII GATC 4 cut(s) 66, 999, 1348, 1371
EaeI YGGCCR 2 cut(s) 1089, 1300
Eam1104I CTCTTC 2 cut(s) 595, 1479
EarI CTCTTC 2 cut(s) 595, 1479
Eco105I TACGTA 1 cut(s) 1287
Eco130I CCWWGG 1 cut(s) 793
Eco147I AGGCCT 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 1365
EcoRII CCWGG 2 cut(s) 407, 752
EcoT14I CCWWGG 1 cut(s) 793
EcoT22I ATGCAT 2 cut(s) 684, 1131
ErhI CCWWGG 1 cut(s) 793
FaqI GGGAC 1 cut(s) 22
FauNDI CATATG 1 cut(s) 695
FbaI TGATCA 2 cut(s) 66, 1371
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 1 cut(s) 1200
FokI GGATG 2 cut(s) 605, 901
Fsp4HI GCNGC 1 cut(s) 1200
FspBI CTAG 3 cut(s) 29, 279, 561
GlaI GCGC 1 cut(s) 669
GluI GCNGC 1 cut(s) 1200
GsaI CCCAGC 1 cut(s) 888
HaeIII GGCC 3 cut(s) 541, 1091, 1302
HapII CCGG 5 cut(s) 106, 333, 431, 582, 740
HhaI GCGC 1 cut(s) 670
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HincII GTYRAC 2 cut(s) 118, 1585
HindII GTYRAC 2 cut(s) 118, 1585
HindIII AAGCTT 2 cut(s) 632, 876
HinfI GANTC 9 cut(s) 341, 404, 644, 837, 947, 953, 1146, 1454, 1522
HpaI GTTAAC 1 cut(s) 1585
HpaII CCGG 5 cut(s) 106, 333, 431, 582, 740
Hpy166II GTNNAC 6 cut(s) 118, 249, 855, 869, 1214, 1585
Hpy8I GTNNAC 6 cut(s) 118, 249, 855, 869, 1214, 1585
Hpy99I CGWCG 2 cut(s) 122, 350
HpyAV CCTTC 3 cut(s) 600, 1130, 1286
HpyCH4III ACNGT 1 cut(s) 1171
HpyCH4IV ACGT 1 cut(s) 1286
HpyF10VI GCNNNNNNNGC 9 cut(s) 152, 203, 674, 683, 767, 875, 1061, 1516, 1625
HpyF3I CTNAG 7 cut(s) 384, 527, 658, 824, 1008, 1386, 1643
HpySE526I ACGT 1 cut(s) 1286
HspAI GCGC 1 cut(s) 668
Kpn2I TCCGGA 1 cut(s) 430
KpnI GGTACC 1 cut(s) 582
Ksp22I TGATCA 2 cut(s) 66, 1371
KspAI GTTAAC 1 cut(s) 1585
Kzo9I GATC 4 cut(s) 66, 999, 1348, 1371
Lsp1109I GCAGC 1 cut(s) 1211
LweI GCATC 5 cut(s) 412, 697, 1023, 1549, 1606
MaeI CTAG 3 cut(s) 29, 279, 561
MaeII ACGT 1 cut(s) 1286
MaeIII GTNAC 1 cut(s) 328
MalI GATC 4 cut(s) 68, 1001, 1350, 1373
MboI GATC 4 cut(s) 66, 999, 1348, 1371
MboII GAAGA 8 cut(s) 612, 716, 773, 1106, 1343, 1358, 1415, 1466
MflI RGATCY 1 cut(s) 1348
MhlI GDGCHC 3 cut(s) 267, 805, 871
MlsI TGGCCA 1 cut(s) 1302
MluCI AATT 6 cut(s) 612, 729, 809, 902, 1492, 1573
MluNI TGGCCA 1 cut(s) 1302
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 2 cut(s) 369, 949
Mox20I TGGCCA 1 cut(s) 1302
Mph1103I ATGCAT 2 cut(s) 684, 1131
MroI TCCGGA 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 1218
MscI TGGCCA 1 cut(s) 1302
MseI TTAA 4 cut(s) 20, 234, 873, 1584
MslI CAYNNNNRTG 2 cut(s) 1367, 1462
Msp20I TGGCCA 1 cut(s) 1302
MspI CCGG 5 cut(s) 106, 333, 431, 582, 740
MspR9I CCNGG 5 cut(s) 106, 334, 409, 583, 754
Mva1269I GAATGC 5 cut(s) 309, 460, 763, 1340, 1486
MvaI CCWGG 2 cut(s) 409, 754
MvnI CGCG 1 cut(s) 1187
MwoI GCNNNNNNNGC 9 cut(s) 152, 203, 674, 683, 767, 875, 1061, 1516, 1625
NciI CCSGG 3 cut(s) 106, 334, 583
NcoI CCATGG 1 cut(s) 793
NdeI CATATG 1 cut(s) 695
NdeII GATC 4 cut(s) 66, 999, 1348, 1371
NlaIV GGNNCC 2 cut(s) 59, 580
NmuCI GTSAC 1 cut(s) 328
NsiI ATGCAT 2 cut(s) 684, 1131
NspI RCATGY 4 cut(s) 104, 686, 1059, 1591
PaeI GCATGC 3 cut(s) 104, 686, 1059
PagI TCATGA 1 cut(s) 1002
PceI AGGCCT 1 cut(s) 541
PciI ACATGT 1 cut(s) 1587
PcsI WCGNNNNNNNCGW 1 cut(s) 455
PctI GAATGC 5 cut(s) 309, 460, 763, 1340, 1486
PdmI GAANNNNTTC 1 cut(s) 1218
PfeI GAWTC 8 cut(s) 404, 644, 837, 947, 953, 1146, 1454, 1522
Pfl23II CGTACG 1 cut(s) 1287
PfoI TCCNGGA 2 cut(s) 407, 752
PkrI GCNGC 1 cut(s) 1201
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
Ppu21I YACGTR 1 cut(s) 1287
PscI ACATGT 1 cut(s) 1587
PshAI GACNNNNGTC 1 cut(s) 123
Psp6I CCWGG 2 cut(s) 407, 752
PspFI CCCAGC 1 cut(s) 884
PspGI CCWGG 2 cut(s) 407, 752
PspLI CGTACG 1 cut(s) 1287
PspN4I GGNNCC 2 cut(s) 59, 580
PsuI RGATCY 1 cut(s) 1348
RsaI GTAC 2 cut(s) 580, 1289
RsaNI GTAC 2 cut(s) 579, 1288
RseI CAYNNNNRTG 2 cut(s) 1367, 1462
SalI GTCGAC 1 cut(s) 116
SaqAI TTAA 4 cut(s) 20, 234, 873, 1584
SatI GCNGC 1 cut(s) 1200
Sau3AI GATC 4 cut(s) 66, 999, 1348, 1371
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 5 cut(s) 106, 334, 409, 583, 754
SduI GDGCHC 3 cut(s) 267, 805, 871
SfaNI GCATC 5 cut(s) 412, 697, 1023, 1549, 1606
SfcI CTRYAG 1 cut(s) 1433
SmiMI CAYNNNNRTG 2 cut(s) 1367, 1462
SmlI CTYRAG 3 cut(s) 46, 266, 1538
SmoI CTYRAG 3 cut(s) 46, 266, 1538
SnaBI TACGTA 1 cut(s) 1287
SphI GCATGC 3 cut(s) 104, 686, 1059
Sse9I AATT 6 cut(s) 612, 729, 809, 902, 1492, 1573
SseBI AGGCCT 1 cut(s) 541
SsiI CCGC 1 cut(s) 1185
SspI AATATT 1 cut(s) 1326
SspMI CTAG 3 cut(s) 29, 279, 561
StuI AGGCCT 1 cut(s) 541
StyD4I CCNGG 5 cut(s) 104, 332, 407, 581, 752
StyI CCWWGG 1 cut(s) 793
TaaI ACNGT 1 cut(s) 1171
TaiI ACGT 1 cut(s) 1289
TaqI TCGA 1 cut(s) 117
TasI AATT 6 cut(s) 612, 729, 809, 902, 1492, 1573
TfiI GAWTC 8 cut(s) 404, 644, 837, 947, 953, 1146, 1454, 1522
Tru1I TTAA 4 cut(s) 20, 234, 873, 1584
Tru9I TTAA 4 cut(s) 20, 234, 873, 1584
TscAI CASTG 2 cut(s) 485, 1381
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 1 cut(s) 1199
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 7 cut(s) 302, 1055, 1211, 1407, 1446, 1514, 1520
TspGWI ACGGA 2 cut(s) 830, 1305
TspRI CASTG 2 cut(s) 485, 1381
VneI GTGCAC 1 cut(s) 867
XapI RAATTY 3 cut(s) 612, 1492, 1573
XceI RCATGY 4 cut(s) 104, 686, 1059, 1591
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 117
XmnI GAANNNNTTC 1 cut(s) 1218
XspI CTAG 3 cut(s) 29, 279, 561
Zsp2I ATGCAT 2 cut(s) 684, 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.