pycom10g24240

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012592
Physical Location & Seq
Forward (+)
26109786 .. 26112572
2787 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g24240.1

Sequence Viewer

Length: 2787 bp
ATGATCAAATGGACCATGAAGCTTTGCTTCTTCTTCGATTCCCTCCAAAACCCTCTTCCCCAACGGCTTTACCTGCCATTTAAACCCATCCAAACCTCACTTCAATTCAGCACTGAGGCAAAAACCCTAAACCCTAATTTCACACCCACTTCTGTATCATACTCTAAGCTCTTGTCACAATGCACTGCTACCAAATCTGTTGGTGTGGGCAGGGAAATCCATGCCCACGTGATCAGATTCGGATGTTCCGAAGACCCAAATCTTCGGAACCATCTGATCAATCTGTACGCGAAATGTCGGTTTTTCAGGCACGCCCGGAAACTGGTTGATGAAAGTCCTGAACCAGATTTGGTGTCTTGGTCTGCTTTGATATCTGGGTATGCCCAAAATGGGCTTGGTAAAGAAGCCCTTTCGGCGTTTCGTGAGATGCACTCGTTGGGTGTTAAGTGTAATGAGTTTACGTTTCCGAGTGTTCTTAAGGCGTGCTCTATTACTAAGGATCTGGGACTGGGGAAGCAGGTTCACGGGGTTGCGCTGCTTACAGGGTTTGAGTCCGATGAGTTTGTTGCGAATACTTTGGTGGTAATGTATTCGAAATGTGGTGAGTTTGGGGATTCTAGGAGGCTCTTTGATGCAATTCCAGAGCGGAATGTTGTTTCGTGGAATGCGTTGTTTTCTTGTTATGTGCAGAGTGATTTCCATGGAGAAGCAATGGATTTGTTTCAGGAAATGGTTCTGAGCGGAGTAAGACCTAATGAGTACAGTCTATCTAGTATAATAAACGCGTGCACTGGGTTCGGGGATGGCAGTCAAGGAAGGAAAATTCATGGTTATATGATAAAGCTCGGATATGAATCTGACTCATTTTCAGCAAATGCACTTGTTGACATGTATGCCAAAGTCAAAAGTCTTGATGATGCAGTAACAGTATTTGAAAAAATCGAACAGCCCGATATTGTTTCCTGGAATGCTGTTATTGCTGGTTGTGTTCTTCACGAGTACCATGGTCGGGCTCTAAAATTTTTTATACAAATGAAAGGGTCAGGAATCCGTCCAAATATGTTCACATTATCGAGCGCACTTAAAGCCTGTGCCGGACTAGGGTTCAAGAAATTGGGTAGGCAGTTGCATTCTTTCTTGATAAAGATGGATATGGAATCAGATTCATTTGTCAATGTTGGACTGATAGATATGTATTGCAAGTGTGAGATGATGAGCAATGCAAGGGTTCTTTTCGATATGATGCCAAAGAAGGACATGATTGCATGGAATGCTGTGATCTCGGGACATTCGCAGAATGGGGAAGATATAGAAGCGGTATCCCTCTTTTCTGAAATGTATAAGGATGGGGTAGAATTTAACCAGACTACCTTATCTACAGTCCTCAAAGCCACGGCGAGCGTGCAGGCCAGAGATGTTTGTAAACAAGTTCATGCACTTTCTGTCAAAACAGGTTTCGAATCTGATATGTATGTTATAAATAGCCTTCTCGATACATATGGAAAATGCGGCAAAGTACAAGACGCGGCAAAAATTTTTAAAGAATGCCCAATTGAGGACGTGGTGGCTTTTACATCGATGATCACAGCCTATTCACAATATGAACAAGGAGAAGAAGCTCTAAAACTCTACTTGCAAATGCTGGAGAGGGAAAACAAACCTGATTCATTTGTATGCAGTTCTCTTCTGAATGCCTGTGCAAATTTGTCTGCATATGAGCAAGGGAAACAGATCCACGTTCACATTCTCAAGTTTGGATTCATGTCTGATGCTTTTGCCGGGAACTCTCTTGTTAACATGTATGCAAAATGTGGAAGCATAGAAGATGCAGATTGTGCTTTCGCTGAGGTACCTGAGAGAGGAATTGTTTCATGGTCTGCAATGATTGGAGGACTCGCTCAACACGGGCATGGAAGAGAGGCCCTCAACTTATTCATTCAGATGCTCAAGGATGGCGTTCCCCCCAATCATATTACTTTAGTAAGTGTCCTCTGTGCGTGCAATCACGCAGGTTTGGTAACGGAAGCCAAAAAGTATTTTGAGTCCATGACAGAGTTGTTTGGAGTTGTGCCGAGGCAAGAGCATTATGCTTGCATGATTGATCTCCTTGGCAGAGCAGGGAAGATAAATGAGGCAATGGAGCTTGTCAAAACAATGCCATTTCAAGCGAACGCGTCTGTTTGGGGGGCACTTCTTGGTGCTGCGAGAATCCATAAAAATGTTGAGCTTGGCGAGCGCGCTGCTGAAATGCTCTTAGTTCTTGAGCCCGAGAAATCTGGTACTCATGTACTTCTTGCAAACATTTACGCATCAGCCGGCATGTGGGATAATGTGGCGAAAATGAGAAAACTTATGAAAGATGACCAGGTAAAGAAGGAACCTGGGATGAGTTGGATTGAGGTCAAAGACCAGGTACACACATTTATAGTAGGAGATAGAAACCATTCAAGAAGTGCTGAAATTTATGCGAAACTCGATGAGCTTTTTGACCTAATGTATAAAGCCGGCTATGTTCCAATGGTGGAGATGGACCTCCATGATGTGGAACAAGGTGAAAAGCAGCGGCTTCTGCGCTACCACAGCGAGAAGCTTGCAGTGGCTTTCGCGTTGATTGCCACTCCGCGGGGAGCTCCCATTAGGGTGAAGAAGAATCTTAGAGTATGCGTGGACTGCCACACAGCACTCAAGTTCATCAGTAAAATAGTTTCCAGGGAGATTATTGTGAGGGACGTCAATAGATTCCATCATTTCAGAGAAGGTTCTTGCTCTTGTAGGGATTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

929

Amino Acids

103.66

Weight (kDa)

6.66

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 115 - 162 1.9e-10 PPR repeat family
PPR PF01535 118 - 148 3.8e-08 PPR repeat
PPR_2 PF13041 216 - 263 2.3e-11 PPR repeat family
PPR PF01535 219 - 249 2.6e-06 PPR repeat
PPR_2 PF13041 317 - 365 5.2e-10 PPR repeat family
PPR_2 PF13041 419 - 465 3.5e-08 PPR repeat family
PPR PF01535 422 - 451 6.1e-06 PPR repeat
PPR_2 PF13041 520 - 568 2.3e-10 PPR repeat family
PPR_2 PF13041 621 - 667 1.3e-09 PPR repeat family
PPR PF01535 623 - 653 6.6e-07 PPR repeat
E_motif PF20431 738 - 800 3.9e-22 E motif
Eplus_motif PF20430 805 - 830 1e-06 E+ motif
DYW_deaminase PF14432 836 - 928 4.9e-41 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1478
AatII GACGTC 1 cut(s) 2733
Acc36I ACCTGC 3 cut(s) 81, 508, 2000
Acc65I GGTACC 1 cut(s) 1849
AccB1I GGYRCC 1 cut(s) 1849
AccB7I CCANNNNNTGG 1 cut(s) 2542
AccBSI CCGCTC 2 cut(s) 646, 741
AccII CGCG 7 cut(s) 290, 785, 1526, 2174, 2238, 2606, 2623
AciI CCGC 8 cut(s) 646, 741, 1316, 1509, 1526, 2563, 2621, 2623
AclWI GGATC 2 cut(s) 507, 1726
AcsI RAATTY 6 cut(s) 822, 1019, 1355, 1533, 1702, 2460
AcvI CACGTG 1 cut(s) 229
AcyI GRCGYC 1 cut(s) 2730
AfaI GTAC 8 cut(s) 287, 761, 1001, 1518, 1851, 2281, 2289, 2415
AfiI CCNNNNNNNGG 9 cut(s) 322, 390, 1009, 1101, 1555, 1859, 2322, 2542, 2622
AflII CTTAAG 1 cut(s) 476
AflIII ACRYGT 4 cut(s) 783, 888, 1797, 2172
AgsI TTSAA 5 cut(s) 104, 935, 1108, 2165, 2448
AjiI CACGTC 1 cut(s) 1561
AjnI CCWGG 5 cut(s) 962, 2364, 2380, 2409, 2708
AjuI GAANNNNNNNTTGG 4 cut(s) 39, 71, 84, 116
AluBI AGCT 9 cut(s) 22, 169, 844, 1619, 2143, 2227, 2482, 2590, 2630
AluI AGCT 9 cut(s) 22, 169, 844, 1619, 2143, 2227, 2482, 2590, 2630
Alw21I GWGCWC 3 cut(s) 488, 791, 2632
Alw44I GTGCAC 1 cut(s) 787
AlwI GGATC 2 cut(s) 507, 1726
Ama87I CYCGRG 2 cut(s) 1282, 2267
AoxI GGCC 2 cut(s) 1407, 1920
ApaLI GTGCAC 1 cut(s) 787
ApeKI GCWGC 4 cut(s) 535, 2201, 2240, 2560
ApoI RAATTY 6 cut(s) 822, 1019, 1355, 1533, 1702, 2460
Asp700I GAANNNNTTC 3 cut(s) 732, 1867, 2443
Asp718I GGTACC 1 cut(s) 1849
AspLEI GCGC 5 cut(s) 535, 1079, 2238, 2240, 2574
AspS9I GGNCC 3 cut(s) 12, 1921, 2530
AsuC2I CCSGG 2 cut(s) 316, 1780
AsuHPI GGTGA 3 cut(s) 614, 2564, 2653
AsuII TTCGAA 2 cut(s) 593, 1458
AvaI CYCGRG 2 cut(s) 1282, 2267
AvaII GGWCC 2 cut(s) 12, 2530
BaeGI GKGCMC 2 cut(s) 791, 2191
BaeI ACNNNNGTAYC 4 cut(s) 138, 171, 2271, 2304
BanI GGYRCC 1 cut(s) 1849
BanII GRGCYC 3 cut(s) 1015, 2268, 2632
BauI CACGAG 1 cut(s) 995
BbrPI CACGTG 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 3 cut(s) 488, 791, 2632
BbvCI CCTCAGC 1 cut(s) 1845
BbvI GCAGC 4 cut(s) 522, 2188, 2227, 2572
BccI CCATC 8 cut(s) 95, 279, 797, 1141, 1340, 1946, 2521, 2751
BceAI ACGGC 2 cut(s) 80, 1410
BcgI CGANNNNNNTGC 6 cut(s) 778, 812, 2222, 2256, 2573, 2607
BciT130I CCWGG 5 cut(s) 964, 2366, 2382, 2411, 2710
BciVI GTATCC 1 cut(s) 1330
BclI TGATCA 4 cut(s) 3, 231, 276, 1581
BcnI CCSGG 2 cut(s) 316, 1780
BfaI CTAG 3 cut(s) 618, 771, 1100
BfmI CTRYAG 1 cut(s) 1377
BfrI CTTAAG 1 cut(s) 476
BfuAI ACCTGC 3 cut(s) 81, 508, 2000
BfuI GTATCC 1 cut(s) 1330
BglI GCCNNNNNGGC 1 cut(s) 413
BisI GCNGC 7 cut(s) 536, 1510, 1527, 2202, 2241, 2561, 2564
BlsI GCNGC 7 cut(s) 537, 1511, 1528, 2203, 2242, 2562, 2565
Bme1390I CCNGG 7 cut(s) 316, 964, 1780, 2366, 2382, 2411, 2710
Bme18I GGWCC 2 cut(s) 12, 2530
BmeT110I CYCGRG 2 cut(s) 1282, 2267
BmgBI CACGTC 1 cut(s) 1561
BmgT120I GGNCC 3 cut(s) 12, 1921, 2530
BmiI GGNNCC 3 cut(s) 269, 1851, 2379
BmrFI CCNGG 7 cut(s) 316, 964, 1780, 2366, 2382, 2411, 2710
BmrI ACTGGG 2 cut(s) 518, 801
BmsI GCATC 8 cut(s) 417, 622, 907, 1233, 1759, 1816, 1932, 2318
BmuI ACTGGG 2 cut(s) 518, 801
BpiI GAAGAC 1 cut(s) 258
BplI GAGNNNNNCTC 4 cut(s) 416, 448, 1908, 1940
BpmI CTGGAG 1 cut(s) 1664
Bpu10I CCTNAGC 1 cut(s) 1845
Bpu14I TTCGAA 2 cut(s) 593, 1458
BpuEI CTTGAG 4 cut(s) 1733, 1931, 2282, 2669
BpuMI CCSGG 2 cut(s) 316, 1780
Bsa29I ATCGAT 1 cut(s) 1577
BsaAI YACGTR 1 cut(s) 229
BsaBI GATNNNNATC 1 cut(s) 853
BsaHI GRCGYC 1 cut(s) 2730
BsaJI CCNNGG 8 cut(s) 700, 1003, 1392, 2072, 2107, 2381, 2621, 2709
Bsc4I CCNNNNNNNGG 9 cut(s) 322, 390, 1009, 1101, 1555, 1859, 2322, 2542, 2622
Bse118I RCCGGY 2 cut(s) 2315, 2504
Bse1I ACTGG 3 cut(s) 327, 513, 796
Bse3DI GCAATG 4 cut(s) 717, 1225, 1887, 2142
Bse8I GATNNNNATC 1 cut(s) 853
BseBI CCWGG 5 cut(s) 964, 2366, 2382, 2411, 2710
BseCI ATCGAT 1 cut(s) 1577
BseDI CCNNGG 8 cut(s) 700, 1003, 1392, 2072, 2107, 2381, 2621, 2709
BseGI GGATG 6 cut(s) 87, 248, 808, 1351, 1957, 2391
BseJI GATNNNNATC 1 cut(s) 853
BseLI CCNNNNNNNGG 9 cut(s) 322, 390, 1009, 1101, 1555, 1859, 2322, 2542, 2622
BseMI GCAATG 4 cut(s) 717, 1225, 1887, 2142
BseMII CTCAG 4 cut(s) 105, 728, 1836, 1845
BseNI ACTGG 3 cut(s) 327, 513, 796
BsePI GCGCGC 1 cut(s) 2236
BseSI GKGCMC 2 cut(s) 791, 2191
BseXI GCAGC 4 cut(s) 522, 2188, 2227, 2572
BsgI GTGCAG 2 cut(s) 707, 1424
Bsh1236I CGCG 7 cut(s) 290, 785, 1526, 2174, 2238, 2606, 2623
BshFI GGCC 2 cut(s) 1409, 1922
BshNI GGYRCC 1 cut(s) 1849
BshVI ATCGAT 1 cut(s) 1577
BsiHKAI GWGCWC 3 cut(s) 488, 791, 2632
BsiHKCI CYCGRG 2 cut(s) 1282, 2267
BsiSI CCGG 5 cut(s) 316, 1095, 1779, 2316, 2505
BslFI GGGAC 3 cut(s) 519, 1299, 2741
BslI CCNNNNNNNGG 9 cut(s) 322, 390, 1009, 1101, 1555, 1859, 2322, 2542, 2622
BsmFI GGGAC 3 cut(s) 519, 1299, 2741
BsmI GAATGC 6 cut(s) 670, 973, 1129, 1276, 1550, 1696
BsnI GGCC 2 cut(s) 1409, 1922
BsoBI CYCGRG 2 cut(s) 1282, 2267
Bsp119I TTCGAA 2 cut(s) 593, 1458
Bsp1286I GDGCHC 6 cut(s) 488, 791, 1015, 2191, 2268, 2632
Bsp143I GATC 8 cut(s) 3, 231, 276, 499, 1278, 1581, 1731, 2101
Bsp19I CCATGG 2 cut(s) 700, 1003
BspACI CCGC 8 cut(s) 646, 741, 1316, 1509, 1526, 2563, 2621, 2623
BspANI GGCC 2 cut(s) 1409, 1922
BspCNI CTCAG 4 cut(s) 106, 729, 1837, 1846
BspDI ATCGAT 1 cut(s) 1577
BspFNI CGCG 7 cut(s) 290, 785, 1526, 2174, 2238, 2606, 2623
BspLI GGNNCC 3 cut(s) 269, 1851, 2379
BspMI ACCTGC 3 cut(s) 81, 508, 2000
BspPI GGATC 2 cut(s) 507, 1726
BspT104I TTCGAA 2 cut(s) 593, 1458
BspT107I GGYRCC 1 cut(s) 1849
BspTI CTTAAG 1 cut(s) 476
BsrBI CCGCTC 2 cut(s) 646, 741
BsrDI GCAATG 4 cut(s) 717, 1225, 1887, 2142
BsrFI RCCGGY 2 cut(s) 2315, 2504
BsrI ACTGG 3 cut(s) 327, 513, 796
BssAI RCCGGY 2 cut(s) 2315, 2504
BssECI CCNNGG 8 cut(s) 700, 1003, 1392, 2072, 2107, 2381, 2621, 2709
BssHII GCGCGC 1 cut(s) 2236
BssMI GATC 8 cut(s) 3, 231, 276, 499, 1278, 1581, 1731, 2101
BssNI GRCGYC 1 cut(s) 2730
BssSI CACGAG 1 cut(s) 995
BssT1I CCWWGG 3 cut(s) 700, 1003, 2107
Bst2BI CACGAG 1 cut(s) 995
Bst2UI CCWGG 5 cut(s) 964, 2366, 2382, 2411, 2710
Bst4CI ACNGT 3 cut(s) 764, 928, 1381
Bst6I CTCTTC 3 cut(s) 60, 1689, 1909
BstACI GRCGYC 1 cut(s) 2730
BstAFI CTTAAG 1 cut(s) 476
BstAPI GCANNNNNTGC 2 cut(s) 1271, 1835
BstBAI YACGTR 1 cut(s) 229
BstBI TTCGAA 2 cut(s) 593, 1458
BstDEI CTNAG 8 cut(s) 114, 165, 495, 737, 1845, 1854, 2254, 2654
BstDSI CCRYGG 4 cut(s) 700, 1003, 1392, 2621
BstENI CCTNNNNNAGG 1 cut(s) 1857
BstF5I GGATG 6 cut(s) 87, 248, 808, 1351, 1957, 2391
BstFNI CGCG 7 cut(s) 290, 785, 1526, 2174, 2238, 2606, 2623
BstHHI GCGC 5 cut(s) 535, 1079, 2238, 2240, 2574
BstKTI GATC 8 cut(s) 6, 234, 279, 502, 1281, 1584, 1734, 2104
BstMBI GATC 8 cut(s) 3, 231, 276, 499, 1278, 1581, 1731, 2101
BstNI CCWGG 5 cut(s) 964, 2366, 2382, 2411, 2710
BstNSI RCATGY 3 cut(s) 892, 1801, 2323
BstSCI CCNGG 7 cut(s) 314, 962, 1778, 2364, 2380, 2409, 2708
BstSFI CTRYAG 1 cut(s) 1377
BstSLI GKGCMC 2 cut(s) 791, 2191
BstUI CGCG 7 cut(s) 290, 785, 1526, 2174, 2238, 2606, 2623
BstV1I GCAGC 4 cut(s) 522, 2188, 2227, 2572
BstV2I GAAGAC 1 cut(s) 258
BstX2I RGATCY 2 cut(s) 499, 1731
BstYI RGATCY 2 cut(s) 499, 1731
Bsu15I ATCGAT 1 cut(s) 1577
BsuI GTATCC 1 cut(s) 1330
BsuRI GGCC 2 cut(s) 1409, 1922
BsuTUI ATCGAT 1 cut(s) 1577
BtgI CCRYGG 4 cut(s) 700, 1003, 1392, 2621
BtrI CACGTC 1 cut(s) 1561
BtsCI GGATG 6 cut(s) 87, 248, 808, 1351, 1957, 2391
BtsI GCAGTG 2 cut(s) 183, 2601
BtsIMutI CAGTG 4 cut(s) 111, 183, 789, 2601
BveI ACCTGC 3 cut(s) 81, 508, 2000
CfoI GCGC 5 cut(s) 535, 1079, 2238, 2240, 2574
Cfr10I RCCGGY 2 cut(s) 2315, 2504
Cfr13I GGNCC 3 cut(s) 12, 1921, 2530
Cfr42I CCGCGG 1 cut(s) 2624
ClaI ATCGAT 1 cut(s) 1577
CseI GACGC 2 cut(s) 1532, 2163
CsiI ACCWGGT 2 cut(s) 2364, 2409
Csp6I GTAC 8 cut(s) 286, 760, 1000, 1517, 1850, 2280, 2288, 2414
CviQI GTAC 8 cut(s) 286, 760, 1000, 1517, 1850, 2280, 2288, 2414
DdeI CTNAG 8 cut(s) 114, 165, 495, 737, 1845, 1854, 2254, 2654
DpnI GATC 8 cut(s) 5, 233, 278, 501, 1280, 1583, 1733, 2103
DpnII GATC 8 cut(s) 3, 231, 276, 499, 1278, 1581, 1731, 2101
DraI TTTAAA 2 cut(s) 82, 1540
Eam1104I CTCTTC 3 cut(s) 60, 1689, 1909
EarI CTCTTC 3 cut(s) 60, 1689, 1909
Ecl136II GAGCTC 1 cut(s) 2630
Eco130I CCWWGG 3 cut(s) 700, 1003, 2107
Eco24I GRGCYC 3 cut(s) 1015, 2268, 2632
Eco32I GATATC 1 cut(s) 372
Eco47I GGWCC 2 cut(s) 12, 2530
Eco53kI GAGCTC 1 cut(s) 2630
Eco72I CACGTG 1 cut(s) 229
Eco88I CYCGRG 2 cut(s) 1282, 2267
EcoICRI GAGCTC 1 cut(s) 2630
EcoNI CCTNNNNNAGG 1 cut(s) 1857
EcoO109I RGGNCCY 1 cut(s) 1921
EcoRII CCWGG 5 cut(s) 962, 2364, 2380, 2409, 2708
EcoRV GATATC 1 cut(s) 372
EcoT14I CCWWGG 3 cut(s) 700, 1003, 2107
EcoT38I GRGCYC 3 cut(s) 1015, 2268, 2632
ErhI CCWWGG 3 cut(s) 700, 1003, 2107
FalI AAGNNNNNCTT 4 cut(s) 11, 43, 393, 425
FaqI GGGAC 3 cut(s) 519, 1299, 2741
FauI CCCGC 1 cut(s) 2616
FauNDI CATATG 2 cut(s) 1498, 1714
FbaI TGATCA 4 cut(s) 3, 231, 276, 1581
Fnu4HI GCNGC 7 cut(s) 536, 1510, 1527, 2202, 2241, 2561, 2564
FokI GGATG 6 cut(s) 74, 255, 815, 1358, 1964, 2398
FriOI GRGCYC 3 cut(s) 1015, 2268, 2632
Fsp4HI GCNGC 7 cut(s) 536, 1510, 1527, 2202, 2241, 2561, 2564
FspBI CTAG 3 cut(s) 618, 771, 1100
GlaI GCGC 5 cut(s) 534, 1078, 2237, 2239, 2573
GluI GCNGC 7 cut(s) 536, 1510, 1527, 2202, 2241, 2561, 2564
GsuI CTGGAG 1 cut(s) 1664
HaeIII GGCC 2 cut(s) 1409, 1922
HapII CCGG 5 cut(s) 316, 1095, 1779, 2316, 2505
HgaI GACGC 2 cut(s) 1532, 2163
HhaI GCGC 5 cut(s) 535, 1079, 2238, 2240, 2574
Hin1I GRCGYC 1 cut(s) 2730
Hin6I GCGC 5 cut(s) 533, 1077, 2236, 2238, 2572
HinP1I GCGC 5 cut(s) 533, 1077, 2236, 2238, 2572
HincII GTYRAC 2 cut(s) 886, 1795
HindII GTYRAC 2 cut(s) 886, 1795
HindIII AAGCTT 2 cut(s) 20, 2588
HpaI GTTAAC 1 cut(s) 1795
HpaII CCGG 5 cut(s) 316, 1095, 1779, 2316, 2505
HphI GGTGA 3 cut(s) 614, 2564, 2653
HpyAV CCTTC 5 cut(s) 810, 1246, 1496, 2368, 2750
HpyCH4III ACNGT 3 cut(s) 764, 928, 1381
HpyCH4IV ACGT 5 cut(s) 228, 461, 1560, 1737, 2730
HpyF3I CTNAG 8 cut(s) 114, 165, 495, 737, 1845, 1854, 2254, 2654
HpySE526I ACGT 5 cut(s) 228, 461, 1560, 1737, 2730
Hsp92I GRCGYC 1 cut(s) 2730
HspAI GCGC 5 cut(s) 533, 1077, 2236, 2238, 2572
KpnI GGTACC 1 cut(s) 1853
KroI GCCGGC 2 cut(s) 2315, 2504
KroNI GCCGGC 2 cut(s) 2317, 2506
Ksp22I TGATCA 4 cut(s) 3, 231, 276, 1581
KspAI GTTAAC 1 cut(s) 1795
KspI CCGCGG 1 cut(s) 2624
Kzo9I GATC 8 cut(s) 3, 231, 276, 499, 1278, 1581, 1731, 2101
LmnI GCTCC 3 cut(s) 2140, 2627, 2635
Lsp1109I GCAGC 4 cut(s) 522, 2188, 2227, 2572
LweI GCATC 8 cut(s) 417, 622, 907, 1233, 1759, 1816, 1932, 2318
MabI ACCWGGT 2 cut(s) 2364, 2409
MaeI CTAG 3 cut(s) 618, 771, 1100
MaeII ACGT 5 cut(s) 228, 461, 1560, 1737, 2730
MaeIII GTNAC 3 cut(s) 174, 922, 2017
MalI GATC 8 cut(s) 5, 233, 278, 501, 1280, 1583, 1733, 2103
MbiI CCGCTC 2 cut(s) 646, 741
MboI GATC 8 cut(s) 3, 231, 276, 499, 1278, 1581, 1731, 2101
MfeI CAATTG 1 cut(s) 1551
MflI RGATCY 2 cut(s) 499, 1731
MhlI GDGCHC 6 cut(s) 488, 791, 1015, 2191, 2268, 2632
MluI ACGCGT 2 cut(s) 783, 2172
MlyI GAGTC 4 cut(s) 560, 854, 1887, 2051
MmeI TCCRAC 2 cut(s) 1159, 2372
MroNI GCCGGC 2 cut(s) 2315, 2504
MroXI GAANNNNTTC 3 cut(s) 732, 1867, 2443
MseI TTAA 7 cut(s) 81, 444, 477, 1083, 1359, 1539, 1794
MslI CAYNNNNRTG 5 cut(s) 1672, 1908, 1940, 2217, 2639
MspA1I CMGCKG 2 cut(s) 2563, 2623
MspCI CTTAAG 1 cut(s) 476
MspI CCGG 5 cut(s) 316, 1095, 1779, 2316, 2505
MspR9I CCNGG 7 cut(s) 316, 964, 1780, 2366, 2382, 2411, 2710
MunI CAATTG 1 cut(s) 1551
Mva1269I GAATGC 6 cut(s) 670, 973, 1129, 1276, 1550, 1696
MvaI CCWGG 5 cut(s) 964, 2366, 2382, 2411, 2710
MvnI CGCG 7 cut(s) 290, 785, 1526, 2174, 2238, 2606, 2623
NaeI GCCGGC 2 cut(s) 2317, 2506
NciI CCSGG 2 cut(s) 316, 1780
NcoI CCATGG 2 cut(s) 700, 1003
NdeI CATATG 2 cut(s) 1498, 1714
NdeII GATC 8 cut(s) 3, 231, 276, 499, 1278, 1581, 1731, 2101
NgoMIV GCCGGC 2 cut(s) 2315, 2504
NlaIV GGNNCC 3 cut(s) 269, 1851, 2379
NmeAIII GCCGAG 1 cut(s) 2097
NmuCI GTSAC 1 cut(s) 174
NspI RCATGY 3 cut(s) 892, 1801, 2323
NspV TTCGAA 2 cut(s) 593, 1458
PauI GCGCGC 1 cut(s) 2236
PciI ACATGT 2 cut(s) 888, 1797
PcsI WCGNNNNNNNCGW 3 cut(s) 246, 665, 948
PctI GAATGC 6 cut(s) 670, 973, 1129, 1276, 1550, 1696
PdiI GCCGGC 2 cut(s) 2317, 2506
PdmI GAANNNNTTC 3 cut(s) 732, 1867, 2443
PflMI CCANNNNNTGG 1 cut(s) 2542
PfoI TCCNGGA 1 cut(s) 962
PkrI GCNGC 7 cut(s) 537, 1511, 1528, 2203, 2242, 2562, 2565
PleI GAGTC 4 cut(s) 559, 854, 1887, 2050
PmaCI CACGTG 1 cut(s) 229
PmlI CACGTG 1 cut(s) 229
PpsI GAGTC 4 cut(s) 559, 854, 1887, 2050
Ppu21I YACGTR 1 cut(s) 229
PscI ACATGT 2 cut(s) 888, 1797
PsiI TTATAA 1 cut(s) 1478
Psp124BI GAGCTC 1 cut(s) 2632
Psp6I CCWGG 5 cut(s) 962, 2364, 2380, 2409, 2708
PspCI CACGTG 1 cut(s) 229
PspGI CCWGG 5 cut(s) 962, 2364, 2380, 2409, 2708
PspN4I GGNNCC 3 cut(s) 269, 1851, 2379
PspPI GGNCC 3 cut(s) 12, 1921, 2530
PsuI RGATCY 2 cut(s) 499, 1731
PteI GCGCGC 1 cut(s) 2236
RsaI GTAC 8 cut(s) 287, 761, 1001, 1518, 1851, 2281, 2289, 2415
RsaNI GTAC 8 cut(s) 286, 760, 1000, 1517, 1850, 2280, 2288, 2414
RseI CAYNNNNRTG 5 cut(s) 1672, 1908, 1940, 2217, 2639
SacI GAGCTC 1 cut(s) 2632
SacII CCGCGG 1 cut(s) 2624
SaqAI TTAA 7 cut(s) 81, 444, 477, 1083, 1359, 1539, 1794
SatI GCNGC 7 cut(s) 536, 1510, 1527, 2202, 2241, 2561, 2564
Sau3AI GATC 8 cut(s) 3, 231, 276, 499, 1278, 1581, 1731, 2101
Sau96I GGNCC 3 cut(s) 12, 1921, 2530
SchI GAGTC 4 cut(s) 560, 854, 1887, 2051
ScrFI CCNGG 7 cut(s) 316, 964, 1780, 2366, 2382, 2411, 2710
SduI GDGCHC 6 cut(s) 488, 791, 1015, 2191, 2268, 2632
SexAI ACCWGGT 2 cut(s) 2364, 2409
SfaNI GCATC 8 cut(s) 417, 622, 907, 1233, 1759, 1816, 1932, 2318
SfcI CTRYAG 1 cut(s) 1377
Sfr303I CCGCGG 1 cut(s) 2624
SfuI TTCGAA 2 cut(s) 593, 1458
SgrBI CCGCGG 1 cut(s) 2624
SinI GGWCC 2 cut(s) 12, 2530
SmiMI CAYNNNNRTG 5 cut(s) 1672, 1908, 1940, 2217, 2639
SmlI CTYRAG 5 cut(s) 476, 1748, 1946, 2261, 2684
SmoI CTYRAG 5 cut(s) 476, 1748, 1946, 2261, 2684
SsiI CCGC 8 cut(s) 646, 741, 1316, 1509, 1526, 2563, 2621, 2623
SspMI CTAG 3 cut(s) 618, 771, 1100
SstI GAGCTC 1 cut(s) 2632
StyD4I CCNGG 7 cut(s) 314, 962, 1778, 2364, 2380, 2409, 2708
StyI CCWWGG 3 cut(s) 700, 1003, 2107
TaaI ACNGT 3 cut(s) 764, 928, 1381
TaiI ACGT 5 cut(s) 231, 464, 1563, 1740, 2733
TaqI TCGA 9 cut(s) 36, 593, 942, 1073, 1236, 1458, 1491, 1577, 2475
TatI WGTACW 3 cut(s) 759, 1516, 2287
TauI GCSGC 3 cut(s) 1512, 1529, 2566
Tru1I TTAA 7 cut(s) 81, 444, 477, 1083, 1359, 1539, 1794
Tru9I TTAA 7 cut(s) 81, 444, 477, 1083, 1359, 1539, 1794
TscAI CASTG 4 cut(s) 118, 190, 796, 2601
TseFI GTSAC 1 cut(s) 174
TseI GCWGC 4 cut(s) 535, 2201, 2240, 2560
Tsp45I GTSAC 1 cut(s) 174
TspGWI ACGGA 2 cut(s) 1040, 2036
TspRI CASTG 4 cut(s) 118, 190, 796, 2601
Van91I CCANNNNNTGG 1 cut(s) 2542
Vha464I CTTAAG 1 cut(s) 476
VneI GTGCAC 1 cut(s) 787
VpaK11BI GGWCC 2 cut(s) 12, 2530
XagI CCTNNNNNAGG 1 cut(s) 1857
XapI RAATTY 6 cut(s) 822, 1019, 1355, 1533, 1702, 2460
XceI RCATGY 3 cut(s) 892, 1801, 2323
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
XmnI GAANNNNTTC 3 cut(s) 732, 1867, 2443
XspI CTAG 3 cut(s) 618, 771, 1100
ZraI GACGTC 1 cut(s) 2731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.