Rh5BG066600

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012592
Physical Location & Seq
Reverse (-)
5491894 .. 5496440
4547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG066600.1

Sequence Viewer

Length: 2820 bp
ATGAAGCTAAGCTTATTCCATTCCCTCCAAAACCCTTGTCTTCCCCAACGACTTTACCTGCCCCTTAAACCCAAAACCCTCCCGACCCAATTTCTAAACTCTGCTAAACACATCCAAACCTTAGCTCAATTCTCCACACACCCACAAACCGCCCCTATCCAAAGCTCACTCGAAACCCACAATTCTATCTCCTTCTCTAAGCTATTGTCGCAATGCGCTGCTTCCAAATCTGTGCGTGTGGGCATGGAAGTCCATGCCCACTTAATCAGATTCGGGTTGTCCCAAGACTCGAGCTTTCGGAACCATTTGATCAATTTCTATGCAAAAAGCAGGATTTTTGGGCATGCCCGGAAGCTGATCGACGAAAGTCCTGAACCAGATTTGGTTGCTTGGTCTGCTTTGATTTCTGGATATGCCCAGAATGGGCTTGGTAGAGAAGTCATTTCAGCTTTTCATGAGATGCATGGATTGGGGGTGACGTGTAATGAGTTTGCATTCCCAAGTGTGCTCAAGGCTTGTTCTAGTTCTAAAGATTTGAGGCTGGGAAAGCAGGTTCATGGGGTGGTGTTGGTTACCGGGTTTGAGTGCGATGAGTTTGTTGCTAATACTTTGGTTGTTATGTATTCGAAATGTGGGGATTTTGGGGATTCCAGGAGGCTGTTTGATGCAATTCCAGAGAGGAATGTTGTTTCGTGGAATGCGTTGTTTTCGTGTTATGTGCAGAGTGATTTCTTTGGGGAGGCAATGGATTTGTTTGAGGAAATGGTTGTGAGTGGAATAAGGCCTAATGAGTATAGTCTGTCTAGTATAATAAATGCATGTACTGGGTTGGGGGATGGTAGAAGAGGAAGGAAAATTCATGGATGTATAGTTAAGCTTGGGTATGATTCGGATTCTTTCTCAGCCAATGCGCTTGTTGACATGTATGCAAAAGCCAAAGGTCTTGAAGATGCAATTTCAGTTTTTGAGAAAATTGCACAACCAGATATTGTATCCTGGAATGCTGTTATTGCTGGTTGTGTTCTTCACAACTGCCATGGTCGGGCGCTCAAATTGTTCAGGCAACTGAGAGGGTCAGGAATCTGTCCAAACATGTTTACATTATCAAGTGCACTTAAAGCTTGTGCTGGGATGGGGTTCGAGAAATTGGGTAGGCAGTTGCACTCTTTCTTGATAAAGATGGATACAGAATCAGATTCATATGTCAAAGTTGGACTGATAGATATGTATTGCAAGTGTGAGATGATGACTGATGCAAGGGTGCTTTTTAATATGATGCCAAAGAAAGACATGATTGCATGGAACGCTGTTATCTCTGGTCATTCACAGATGGCTGAAGACAGAGAAGCTGTAACCCTCTTTCCTGAAATGCATAGGGAAGGAATAGGATTCAATGAGACTACTTTGTCTACAGTCCTCAAATCCACCGCGAGCTTGCAGGCAGTAAAAGTTTGTGAACAAGTTCATGCTCCTTCAATCAAAACAGGTTTTCAGTCTGACATGTATGTTATAAATAGCCTTCTTGATACATATGGAAAATGTGGCCAAGTAGAAAATGCAGTAAGAATTTTTGAAGAATGCAAAACTGAAGATTTGGTGGCTTTCACATCAATGATCACTGCCTATGCTCAATATGAACAAGGCGAAGAAGCTCTTAAACTCTATGTGCAAATGCTAATGAAGGGGAACAAGCCTGATTCATTTGTATGCAGTTCCCTTCTGAATGCTTGTGCAAATTTGTCGGCATATGAGCAAGGGAAGCAGATTCATGTTCATATTCTGAAGTTTGGATTTTTGTCTGATGCTTTTGCCGGAAATTCCCTTGTTAATATGTATGCAAAATGTGGAAGCATAGATGATGCGGGTCGTGCTTTCTCCGAGGTACCTGAGAGAGGAATAGTTTCATGGTCTGCAATGATTGGAGGACTTGCTCAACACGGACATGGAAAAGAAGCCCTCAATATGTTCAATCAGATGCTTGGAGATGGTATTTGCCCCAATCATATTACTTTAGTCAGTGTCCTCTGTGCATGCAATCATGCAGGTTTGGTAACTGAAGCCAGAAAATATTTTGAGTCAATGAAAGAGTTGTTTGGGGTTGTACCAAGGGAGGAGCATTATGCGTGCATGATTGATCTTCTTGGCAGAGCAGGAAAGATAAAAGAGGCAATGGAGCTAGTCAACACGATGCCATTTCAAGCAAACGCCTCAGTTTGGGGGTCACTTCTTGGTGCTGCAAGAATCCATAAAAATGTTGAGCTTGGTGAACGTGCTGCTGATATGCTCTTAGTTCTGGAGCCTGAGAAATCTGGTACCCATGTACTTCTTGCAAACATTTATGCAGCAGCTGGCATGTGGGACAAGGTAGCAAAAATGAGAAGACTTATGAAAAATAACCAGGTAAAGAAGGAACCTGGAATGAGTTGGATTGAGGTCCAAGACAAGGTGCACACCTTTATTGTAGGAGATAGAAGTCATTCAAGAAGCACGGAGATATATGCAAAACTTGATGAGCTGCTTGACCTTGTGCATAAAGCTGGCTATGTTCCAATGGTGGAGATTGACCTCCATGATGTGGAACAAAGTGAGAAGGAGCAACTTCTGCGATACCACAGTGAGAAGCTTGCAGTAGCTTTCGCATTGATTGTCACTCCACCTGGAGCTCCCATTAGAGTGAAGAAGAATTTAAGAGTATGTGTAGATTGCCATACTGCATTCAAGTTCATCTGTAAAATTGTCTCGAGGGAGATAATCGTGAGGGACATCAATAGATTCCATCATTTCAAAGATGGCTCATGCTCTTGTGGGGATTACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

939

Amino Acids

104.2

Weight (kDa)

7.05

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 126 - 174 7e-07 PPR repeat family
PPR PF01535 129 - 159 3e-06 PPR repeat
PPR_2 PF13041 227 - 274 6e-12 PPR repeat family
PPR PF01535 230 - 260 5.7e-07 PPR repeat
PPR_2 PF13041 328 - 376 1.7e-08 PPR repeat family
PPR_2 PF13041 531 - 579 7.8e-10 PPR repeat family
PPR_2 PF13041 632 - 678 8.3e-09 PPR repeat family
PPR PF01535 634 - 664 5.9e-07 PPR repeat
E_motif PF20431 749 - 811 8.9e-22 E motif
Eplus_motif PF20430 816 - 841 2.3e-07 E+ motif
DYW_deaminase PF14432 847 - 939 3e-41 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1511
AasI GACNNNNNNGTC 1 cut(s) 1405
Acc36I ACCTGC 3 cut(s) 66, 541, 2033
Acc65I GGTACC 2 cut(s) 1882, 2312
AccB1I GGYRCC 2 cut(s) 1882, 2312
AccB7I CCANNNNNTGG 1 cut(s) 2575
AccI GTMKAC 1 cut(s) 1409
AccII CGCG 1 cut(s) 1430
AciI CCGC 3 cut(s) 150, 1428, 1862
AcoI YGGCCR 1 cut(s) 1543
AcsI RAATTY 5 cut(s) 855, 1566, 1735, 1816, 2683
AcuI CTGAAG 4 cut(s) 1356, 1608, 1802, 2076
AfaI GTAC 5 cut(s) 823, 1884, 2103, 2314, 2322
AfiI CCNNNNNNNGG 6 cut(s) 423, 1042, 1892, 2214, 2443, 2575
AflIII ACRYGT 4 cut(s) 479, 921, 1092, 1500
AgsI TTSAA 9 cut(s) 947, 1393, 1476, 1574, 1969, 2198, 2481, 2719, 2785
AjiI CACGTC 1 cut(s) 480
AjnI CCWGG 5 cut(s) 650, 995, 2397, 2413, 2656
AloI GAACNNNNNNTCC 2 cut(s) 537, 569
Alw21I GWGCWC 4 cut(s) 510, 1114, 2451, 2665
Alw26I GTCTC 2 cut(s) 1391, 2743
Alw44I GTGCAC 2 cut(s) 1110, 2447
AlwNI CAGNNNCTG 1 cut(s) 2348
Ama87I CYCGRG 2 cut(s) 289, 2740
AoxI GGCC 2 cut(s) 782, 1543
ApaLI GTGCAC 2 cut(s) 1110, 2447
ApeKI GCWGC 6 cut(s) 218, 2234, 2273, 2342, 2345, 2515
ApoI RAATTY 5 cut(s) 855, 1566, 1735, 1816, 2683
ArsI GACNNNNNNTTYG 2 cut(s) 22, 54
Asp700I GAANNNNTTC 3 cut(s) 1461, 1900, 2476
Asp718I GGTACC 2 cut(s) 1882, 2312
AspLEI GCGC 3 cut(s) 218, 913, 1048
AspS9I GGNCC 1 cut(s) 2434
AsuC2I CCSGG 2 cut(s) 349, 577
AsuHPI GGTGA 2 cut(s) 487, 2276
AsuII TTCGAA 1 cut(s) 626
AvaI CYCGRG 2 cut(s) 289, 2740
AvaII GGWCC 1 cut(s) 2434
BaeGI GKGCMC 2 cut(s) 1114, 2451
BaeI ACNNNNGTAYC 4 cut(s) 2304, 2337, 2599, 2632
BalI TGGCCA 1 cut(s) 1545
BanI GGYRCC 2 cut(s) 1882, 2312
BanII GRGCYC 1 cut(s) 2665
BbsI GAAGAC 3 cut(s) 32, 1344, 2386
Bbv12I GWGCWC 4 cut(s) 510, 1114, 2451, 2665
BbvI GCAGC 6 cut(s) 205, 2221, 2260, 2354, 2357, 2502
BccI CCATC 7 cut(s) 830, 1126, 1174, 1324, 1979, 2783, 2784
BcgI CGANNNNNNTGC 2 cut(s) 1722, 1756
BciT130I CCWGG 5 cut(s) 652, 997, 2399, 2415, 2658
BciVI GTATCC 2 cut(s) 1003, 1177
BclI TGATCA 2 cut(s) 309, 1614
BcnI CCSGG 2 cut(s) 349, 577
BcoDI GTCTC 2 cut(s) 1391, 2743
BfaI CTAG 3 cut(s) 522, 804, 2177
BfmI CTRYAG 1 cut(s) 1410
BfoI RGCGCY 1 cut(s) 1049
BfuAI ACCTGC 3 cut(s) 66, 541, 2033
BfuI GTATCC 2 cut(s) 1003, 1177
BisI GCNGC 6 cut(s) 219, 2235, 2274, 2343, 2346, 2516
BlpI GCTNAGC 1 cut(s) 8
BlsI GCNGC 6 cut(s) 220, 2236, 2275, 2344, 2347, 2517
Bme1390I CCNGG 7 cut(s) 349, 577, 652, 997, 2399, 2415, 2658
Bme18I GGWCC 1 cut(s) 2434
BmeT110I CYCGRG 2 cut(s) 289, 2740
BmgBI CACGTC 1 cut(s) 480
BmgT120I GGNCC 1 cut(s) 2434
BmiI GGNNCC 5 cut(s) 302, 1884, 2298, 2314, 2412
BmrFI CCNGG 7 cut(s) 349, 577, 652, 997, 2399, 2415, 2658
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 9 cut(s) 450, 655, 940, 1243, 1266, 1792, 1849, 1965, 2178
BmuI ACTGGG 1 cut(s) 834
BoxI GACNNNNGTC 1 cut(s) 366
BpiI GAAGAC 3 cut(s) 32, 1344, 2386
BpmI CTGGAG 2 cut(s) 2315, 2679
Bpu10I CCTNAGC 1 cut(s) 121
Bpu1102I GCTNAGC 1 cut(s) 8
Bpu14I TTCGAA 1 cut(s) 626
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 2 cut(s) 349, 577
BsaJI CCNNGG 3 cut(s) 1036, 1878, 2105
Bsc4I CCNNNNNNNGG 6 cut(s) 423, 1042, 1892, 2214, 2443, 2575
Bse1I ACTGG 2 cut(s) 829, 2819
Bse3DI GCAATG 4 cut(s) 218, 750, 1920, 2175
BseBI CCWGG 5 cut(s) 652, 997, 2399, 2415, 2658
BseDI CCNNGG 3 cut(s) 1036, 1878, 2105
BseGI GGATG 4 cut(s) 111, 841, 869, 1137
BseLI CCNNNNNNNGG 6 cut(s) 423, 1042, 1892, 2214, 2443, 2575
BseMI GCAATG 4 cut(s) 218, 750, 1920, 2175
BseMII CTCAG 5 cut(s) 915, 1058, 1878, 2223, 2292
BseNI ACTGG 2 cut(s) 829, 2819
BseRI GAGGAG 1 cut(s) 2126
BseSI GKGCMC 2 cut(s) 1114, 2451
BseXI GCAGC 6 cut(s) 205, 2221, 2260, 2354, 2357, 2502
BseYI CCCAGC 2 cut(s) 541, 1127
BsgI GTGCAG 1 cut(s) 740
Bsh1236I CGCG 1 cut(s) 1430
BshFI GGCC 2 cut(s) 784, 1545
BshNI GGYRCC 2 cut(s) 1882, 2312
BsiHKAI GWGCWC 4 cut(s) 510, 1114, 2451, 2665
BsiHKCI CYCGRG 2 cut(s) 289, 2740
BsiSI CCGG 3 cut(s) 349, 576, 1812
BslFI GGGAC 3 cut(s) 265, 2372, 2774
BslI CCNNNNNNNGG 6 cut(s) 423, 1042, 1892, 2214, 2443, 2575
BsmAI GTCTC 2 cut(s) 1391, 2743
BsmFI GGGAC 3 cut(s) 265, 2372, 2774
BsmI GAATGC 6 cut(s) 494, 703, 1006, 1583, 1729, 2714
BsnI GGCC 2 cut(s) 784, 1545
BsoBI CYCGRG 2 cut(s) 289, 2740
Bsp119I TTCGAA 1 cut(s) 626
Bsp1286I GDGCHC 4 cut(s) 510, 1114, 2451, 2665
Bsp143I GATC 4 cut(s) 309, 357, 1614, 2134
Bsp1720I GCTNAGC 1 cut(s) 8
Bsp19I CCATGG 1 cut(s) 1036
BspACI CCGC 3 cut(s) 150, 1428, 1862
BspANI GGCC 2 cut(s) 784, 1545
BspCNI CTCAG 5 cut(s) 914, 1059, 1879, 2222, 2293
BspFNI CGCG 1 cut(s) 1430
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 5 cut(s) 302, 1884, 2298, 2314, 2412
BspMI ACCTGC 3 cut(s) 66, 541, 2033
BspT104I TTCGAA 1 cut(s) 626
BspT107I GGYRCC 2 cut(s) 1882, 2312
BsrDI GCAATG 4 cut(s) 218, 750, 1920, 2175
BsrI ACTGG 2 cut(s) 829, 2819
BssECI CCNNGG 3 cut(s) 1036, 1878, 2105
BssMI GATC 4 cut(s) 309, 357, 1614, 2134
BssT1I CCWWGG 2 cut(s) 1036, 2105
Bst2UI CCWGG 5 cut(s) 652, 997, 2399, 2415, 2658
Bst4CI ACNGT 2 cut(s) 1414, 2615
Bst6I CTCTTC 1 cut(s) 838
BstAPI GCANNNNNTGC 1 cut(s) 2602
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 9 cut(s) 345, 1432, 1436, 1440, 2032, 2125, 2350, 2539, 2625
BstDEI CTNAG 9 cut(s) 8, 121, 198, 901, 1067, 1887, 2209, 2287, 2301
BstDSI CCRYGG 1 cut(s) 1036
BstEII GGTNACC 1 cut(s) 571
BstENI CCTNNNNNAGG 1 cut(s) 1890
BstF5I GGATG 4 cut(s) 111, 841, 869, 1137
BstFNI CGCG 1 cut(s) 1430
BstH2I RGCGCY 1 cut(s) 1049
BstHHI GCGC 3 cut(s) 218, 913, 1048
BstKTI GATC 4 cut(s) 312, 360, 1617, 2137
BstMAI GTCTC 2 cut(s) 1391, 2743
BstMBI GATC 4 cut(s) 309, 357, 1614, 2134
BstMWI GCNNNNNNNGC 9 cut(s) 208, 395, 547, 1010, 1118, 1304, 1759, 1868, 2602
BstNI CCWGG 5 cut(s) 652, 997, 2399, 2415, 2658
BstNSI RCATGY 7 cut(s) 347, 822, 925, 1096, 1504, 2034, 2356
BstPAI GACNNNNGTC 1 cut(s) 366
BstPI GGTNACC 1 cut(s) 571
BstSCI CCNGG 7 cut(s) 347, 575, 650, 995, 2397, 2413, 2656
BstSFI CTRYAG 1 cut(s) 1410
BstSLI GKGCMC 2 cut(s) 1114, 2451
BstUI CGCG 1 cut(s) 1430
BstV1I GCAGC 6 cut(s) 205, 2221, 2260, 2354, 2357, 2502
BstV2I GAAGAC 3 cut(s) 32, 1344, 2386
BsuI GTATCC 2 cut(s) 1003, 1177
BsuRI GGCC 2 cut(s) 784, 1545
BtgI CCRYGG 1 cut(s) 1036
BtgZI GCGATG 1 cut(s) 603
BtrI CACGTC 1 cut(s) 480
BtsCI GGATG 4 cut(s) 111, 841, 869, 1137
BtsI GCAGTG 1 cut(s) 1617
BtsIMutI CAGTG 3 cut(s) 1617, 2023, 2620
BveI ACCTGC 3 cut(s) 66, 541, 2033
Cac8I GCNNGC 9 cut(s) 345, 1432, 1436, 1440, 2032, 2125, 2350, 2539, 2625
CaiI CAGNNNCTG 1 cut(s) 2348
CciI TCATGA 1 cut(s) 454
CfoI GCGC 3 cut(s) 218, 913, 1048
Cfr13I GGNCC 1 cut(s) 2434
CsiI ACCWGGT 1 cut(s) 2397
Csp6I GTAC 5 cut(s) 822, 1883, 2102, 2313, 2321
CviQI GTAC 5 cut(s) 822, 1883, 2102, 2313, 2321
DdeI CTNAG 9 cut(s) 8, 121, 198, 901, 1067, 1887, 2209, 2287, 2301
DpnI GATC 4 cut(s) 311, 359, 1616, 2136
DpnII GATC 4 cut(s) 309, 357, 1614, 2134
DrdI GACNNNNNNGTC 1 cut(s) 1405
DseDI GACNNNNNNGTC 1 cut(s) 1405
EaeI YGGCCR 1 cut(s) 1543
Eam1104I CTCTTC 1 cut(s) 838
EarI CTCTTC 1 cut(s) 838
Ecl136II GAGCTC 1 cut(s) 2663
Eco130I CCWWGG 2 cut(s) 1036, 2105
Eco147I AGGCCT 1 cut(s) 784
Eco24I GRGCYC 1 cut(s) 2665
Eco47I GGWCC 1 cut(s) 2434
Eco53kI GAGCTC 1 cut(s) 2663
Eco57I CTGAAG 4 cut(s) 1356, 1608, 1802, 2076
Eco88I CYCGRG 2 cut(s) 289, 2740
Eco91I GGTNACC 1 cut(s) 571
EcoICRI GAGCTC 1 cut(s) 2663
EcoNI CCTNNNNNAGG 1 cut(s) 1890
EcoO65I GGTNACC 1 cut(s) 571
EcoRII CCWGG 5 cut(s) 650, 995, 2397, 2413, 2656
EcoT14I CCWWGG 2 cut(s) 1036, 2105
EcoT22I ATGCAT 3 cut(s) 465, 820, 1374
EcoT38I GRGCYC 1 cut(s) 2665
ErhI CCWWGG 2 cut(s) 1036, 2105
FalI AAGNNNNNCTT 3 cut(s) 28, 1638, 1670
FaqI GGGAC 3 cut(s) 265, 2372, 2774
FauI CCCGC 1 cut(s) 1855
FauNDI CATATG 3 cut(s) 1201, 1531, 1747
FbaI TGATCA 2 cut(s) 309, 1614
FblI GTMKAC 1 cut(s) 1409
Fnu4HI GCNGC 6 cut(s) 219, 2235, 2274, 2343, 2346, 2516
FokI GGATG 4 cut(s) 98, 848, 876, 1144
FriOI GRGCYC 1 cut(s) 2665
Fsp4HI GCNGC 6 cut(s) 219, 2235, 2274, 2343, 2346, 2516
FspBI CTAG 3 cut(s) 522, 804, 2177
GlaI GCGC 3 cut(s) 217, 912, 1047
GluI GCNGC 6 cut(s) 219, 2235, 2274, 2343, 2346, 2516
GsaI CCCAGC 2 cut(s) 545, 1131
GsuI CTGGAG 2 cut(s) 2315, 2679
HaeII RGCGCY 1 cut(s) 1049
HaeIII GGCC 2 cut(s) 784, 1545
HapII CCGG 3 cut(s) 349, 576, 1812
HhaI GCGC 3 cut(s) 218, 913, 1048
Hin6I GCGC 3 cut(s) 216, 911, 1046
HinP1I GCGC 3 cut(s) 216, 911, 1046
HincII GTYRAC 2 cut(s) 919, 2182
HindII GTYRAC 2 cut(s) 919, 2182
HindIII AAGCTT 4 cut(s) 10, 875, 1119, 2621
HpaII CCGG 3 cut(s) 349, 576, 1812
HphI GGTGA 2 cut(s) 487, 2276
Hpy166II GTNNAC 8 cut(s) 919, 1098, 1112, 1410, 1457, 2182, 2267, 2449
Hpy8I GTNNAC 8 cut(s) 919, 1098, 1112, 1410, 1457, 2182, 2267, 2449
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 9 cut(s) 202, 843, 1373, 1482, 1529, 1675, 1727, 2401, 2584
HpyCH4III ACNGT 2 cut(s) 1414, 2615
HpyCH4IV ACGT 2 cut(s) 479, 2269
HpyF10VI GCNNNNNNNGC 9 cut(s) 208, 395, 547, 1010, 1118, 1304, 1759, 1868, 2602
HpyF3I CTNAG 9 cut(s) 8, 121, 198, 901, 1067, 1887, 2209, 2287, 2301
HpySE526I ACGT 2 cut(s) 479, 2269
HspAI GCGC 3 cut(s) 216, 911, 1046
KpnI GGTACC 2 cut(s) 1886, 2316
Ksp22I TGATCA 2 cut(s) 309, 1614
Kzo9I GATC 4 cut(s) 309, 357, 1614, 2134
LmnI GCTCC 7 cut(s) 1474, 2113, 2173, 2296, 2593, 2660, 2668
Lsp1109I GCAGC 6 cut(s) 205, 2221, 2260, 2354, 2357, 2502
LweI GCATC 9 cut(s) 450, 655, 940, 1243, 1266, 1792, 1849, 1965, 2178
MabI ACCWGGT 1 cut(s) 2397
MaeI CTAG 3 cut(s) 522, 804, 2177
MaeII ACGT 2 cut(s) 479, 2269
MaeIII GTNAC 6 cut(s) 475, 571, 1351, 2050, 2220, 2647
MalI GATC 4 cut(s) 311, 359, 1616, 2136
MboI GATC 4 cut(s) 309, 357, 1614, 2134
MhlI GDGCHC 4 cut(s) 510, 1114, 2451, 2665
MlsI TGGCCA 1 cut(s) 1545
MluNI TGGCCA 1 cut(s) 1545
MlyI GAGTC 2 cut(s) 281, 2084
MmeI TCCRAC 2 cut(s) 1192, 2405
Mox20I TGGCCA 1 cut(s) 1545
Mph1103I ATGCAT 3 cut(s) 465, 820, 1374
MroXI GAANNNNTTC 3 cut(s) 1461, 1900, 2476
MscI TGGCCA 1 cut(s) 1545
MseI TTAA 8 cut(s) 66, 263, 873, 1116, 1269, 1656, 1827, 2687
MslI CAYNNNNRTG 5 cut(s) 1610, 1705, 1941, 2250, 2672
Msp20I TGGCCA 1 cut(s) 1545
MspA1I CMGCKG 1 cut(s) 2348
MspI CCGG 3 cut(s) 349, 576, 1812
MspR9I CCNGG 7 cut(s) 349, 577, 652, 997, 2399, 2415, 2658
Mva1269I GAATGC 6 cut(s) 494, 703, 1006, 1583, 1729, 2714
MvaI CCWGG 5 cut(s) 652, 997, 2399, 2415, 2658
MvnI CGCG 1 cut(s) 1430
MwoI GCNNNNNNNGC 9 cut(s) 208, 395, 547, 1010, 1118, 1304, 1759, 1868, 2602
NciI CCSGG 2 cut(s) 349, 577
NcoI CCATGG 1 cut(s) 1036
NdeI CATATG 3 cut(s) 1201, 1531, 1747
NdeII GATC 4 cut(s) 309, 357, 1614, 2134
NlaIV GGNNCC 5 cut(s) 302, 1884, 2298, 2314, 2412
NmuCI GTSAC 3 cut(s) 475, 2220, 2647
NsiI ATGCAT 3 cut(s) 465, 820, 1374
NspI RCATGY 7 cut(s) 347, 822, 925, 1096, 1504, 2034, 2356
NspV TTCGAA 1 cut(s) 626
PaeI GCATGC 2 cut(s) 347, 2034
PaeR7I CTCGAG 2 cut(s) 289, 2740
PagI TCATGA 1 cut(s) 454
PceI AGGCCT 1 cut(s) 784
PciI ACATGT 3 cut(s) 921, 1092, 1500
PcsI WCGNNNNNNNCGW 1 cut(s) 698
PctI GAATGC 6 cut(s) 494, 703, 1006, 1583, 1729, 2714
PdmI GAANNNNTTC 3 cut(s) 1461, 1900, 2476
PflMI CCANNNNNTGG 1 cut(s) 2575
PfoI TCCNGGA 2 cut(s) 650, 995
PkrI GCNGC 6 cut(s) 220, 2236, 2275, 2344, 2347, 2517
PleI GAGTC 2 cut(s) 281, 2083
PpsI GAGTC 2 cut(s) 281, 2083
PscI ACATGT 3 cut(s) 921, 1092, 1500
PshAI GACNNNNGTC 1 cut(s) 366
PsiI TTATAA 1 cut(s) 1511
Psp124BI GAGCTC 1 cut(s) 2665
Psp6I CCWGG 5 cut(s) 650, 995, 2397, 2413, 2656
PspEI GGTNACC 1 cut(s) 571
PspFI CCCAGC 2 cut(s) 541, 1127
PspGI CCWGG 5 cut(s) 650, 995, 2397, 2413, 2656
PspN4I GGNNCC 5 cut(s) 302, 1884, 2298, 2314, 2412
PspPI GGNCC 1 cut(s) 2434
PspXI VCTCGAGB 1 cut(s) 289
PstNI CAGNNNCTG 1 cut(s) 2348
PvuII CAGCTG 1 cut(s) 2348
RsaI GTAC 5 cut(s) 823, 1884, 2103, 2314, 2322
RsaNI GTAC 5 cut(s) 822, 1883, 2102, 2313, 2321
RseI CAYNNNNRTG 5 cut(s) 1610, 1705, 1941, 2250, 2672
SacI GAGCTC 1 cut(s) 2665
SaqAI TTAA 8 cut(s) 66, 263, 873, 1116, 1269, 1656, 1827, 2687
SatI GCNGC 6 cut(s) 219, 2235, 2274, 2343, 2346, 2516
Sau3AI GATC 4 cut(s) 309, 357, 1614, 2134
Sau96I GGNCC 1 cut(s) 2434
SchI GAGTC 2 cut(s) 281, 2084
ScrFI CCNGG 7 cut(s) 349, 577, 652, 997, 2399, 2415, 2658
SduI GDGCHC 4 cut(s) 510, 1114, 2451, 2665
SexAI ACCWGGT 1 cut(s) 2397
SfaNI GCATC 9 cut(s) 450, 655, 940, 1243, 1266, 1792, 1849, 1965, 2178
SfcI CTRYAG 1 cut(s) 1410
Sfr274I CTCGAG 2 cut(s) 289, 2740
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 1 cut(s) 2434
SlaI CTCGAG 2 cut(s) 289, 2740
SmiMI CAYNNNNRTG 5 cut(s) 1610, 1705, 1941, 2250, 2672
SmlI CTYRAG 3 cut(s) 289, 509, 2740
SmoI CTYRAG 3 cut(s) 289, 509, 2740
SphI GCATGC 2 cut(s) 347, 2034
SseBI AGGCCT 1 cut(s) 784
SsiI CCGC 3 cut(s) 150, 1428, 1862
SspI AATATT 1 cut(s) 2069
SspMI CTAG 3 cut(s) 522, 804, 2177
SstI GAGCTC 1 cut(s) 2665
StuI AGGCCT 1 cut(s) 784
StyD4I CCNGG 7 cut(s) 347, 575, 650, 995, 2397, 2413, 2656
StyI CCWWGG 2 cut(s) 1036, 2105
TaaI ACNGT 2 cut(s) 1414, 2615
TaiI ACGT 2 cut(s) 482, 2272
TaqI TCGA 6 cut(s) 171, 290, 360, 626, 1140, 2741
TatI WGTACW 2 cut(s) 821, 2320
Tru1I TTAA 8 cut(s) 66, 263, 873, 1116, 1269, 1656, 1827, 2687
Tru9I TTAA 8 cut(s) 66, 263, 873, 1116, 1269, 1656, 1827, 2687
TscAI CASTG 3 cut(s) 1624, 2023, 2620
TseFI GTSAC 3 cut(s) 475, 2220, 2647
TseI GCWGC 6 cut(s) 218, 2234, 2273, 2342, 2345, 2515
Tsp45I GTSAC 3 cut(s) 475, 2220, 2647
TspGWI ACGGA 2 cut(s) 1953, 2504
TspRI CASTG 3 cut(s) 1624, 2023, 2620
Van91I CCANNNNNTGG 1 cut(s) 2575
VneI GTGCAC 2 cut(s) 1110, 2447
VpaK11BI GGWCC 1 cut(s) 2434
XagI CCTNNNNNAGG 1 cut(s) 1890
XapI RAATTY 5 cut(s) 855, 1566, 1735, 1816, 2683
XceI RCATGY 7 cut(s) 347, 822, 925, 1096, 1504, 2034, 2356
XcmI CCANNNNNNNNNTGG 1 cut(s) 425
XhoI CTCGAG 2 cut(s) 289, 2740
XmiI GTMKAC 1 cut(s) 1409
XmnI GAANNNNTTC 3 cut(s) 1461, 1900, 2476
XspI CTAG 3 cut(s) 522, 804, 2177
Zsp2I ATGCAT 3 cut(s) 465, 820, 1374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.