FvH4_3g11650

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015572
Physical Location & Seq
Forward (+)
6882724 .. 6885136
2413 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11650.t1

Sequence Viewer

Length: 1614 bp
ATGTTCCACGATCCTCACCACAACATTGGTCTAAAACTTACGAGCACCATTCTTACACACGCCGTCACACGTGTGACGAAGCAGTACACTTACCTCCTCCTATATAAAGCCCTCATAGTGCTTCTCTACCCATCATCTCCATCAACAATCTCCAACTACAAAAAAACTCGACTCTCTGAGCTCTCACCTACTACTAAACAAAAACCAAAGCTAGCTCTCCAGTACTTCTACTTCGTACGGTTAATTCGCATTGAATTCAGTGCAATGGCGGCAGCTCTATCCACATCTCCACTGTTCAACATCGACTCCGACTCCGTCGACGTCGACAAACTCAGCCACGAGATCTTCTCCATTCTCGAGAACAAGTTCCTCTTCGGCTACGACATTGATAACGCTACCACCACCACCACCACCACCACCACTCCTCTTCATCACATGAAGTCTAGCAAGCACATCACCGGCAAGGTTAGAATCTTAACCATCGACGGCGCCGGCGCCACCGACGGCATTTTGGCCGCCAAGTCTCTCGTTCACCTCGAATCCTGCATCCGCCACAAGTCCGGCGACTCCACCGCCGCCGTGTCTGACTACTTCGACGTTGTTTCTGGTTCCGGTGTCGGTGGCGTTCTCGCGGCTCTGCTGTTTACTAAAGCGGCAGATGGATCCGGCCGGCCGGTGTTCACTGCTCAGGGGGCACTCAACTTCCTTCTGGAAAACCGAAGCAAGATTTTCAAGTCGTCTCCGGCGGGGGTCTTCCGGCGAGTTTTCCGGCCGGCGAAGGCGGAGAAGGAGAGGGAGAGGCTCTTCCGCAAGACGTTTGGTGACCAGTTGACTATGAAGGACACCCTTAAGGCGGTGTTGATTCCCTGCTACGACATGTCGTCCCGCGCGCCTTTTCTGTTTTCGCGCGCCGACGCTGTGGAGATGGACAGCTACGATTTCAAAATGAAGGACGTGTGCACCGCGACGTGCGCGCACCCGGCTGCGGTGGGGGCTGTGGAGGTTAAGTCAGTCGACGGGAGAACGAAGATCATGGCCGTCGACGGCGGAATTGCCATGAACAATCCGACGTCCGCGGCTATTACACACGTGTTGAATAACAAGCAAGAGTTTCCTTTCTGTTCTGGTGTGGAGGATCTCTTGGTGGTGTCACTCGGAAATGGAGAGTCGGAGTTCGGAAACTTGTCGCCTTCTGGCTGCTTGAGAATCGCTGGCGAAGGAGCTTCCGACTTGGTTGATCAAGCTGTTTCAATGGCATTTGGGCAATCCCGGACCAGCAATTATGTTCGCGTGCAAGCCACCGGCATCATCGCCAAAAACCCTGCACGGATGGACCACTCCAACAACAACAAGAAGAACACCAACACAGACATATTGGCTTTGACAGACGAAATGCTGGAACAGAGGAATGTGGAGTCGATTTTATTCGAGGGGAAGAGAATGGTGGAGAGCACGAACTTGAAGAAGTTGGAGTTGTACGCCGGCGAGGTGATCAAGGAAGAAGAGAGGAGAAAGACGAGCATTTTGCCGACGGTGGCTTTGAAGCAGACTTCAGGGTCGCCAGCCAGAACATCATCTGCTACCACACTATCGAGTCTGTCATCGTCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

538

Amino Acids

58.48

Weight (kDa)

9.0

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 160 - 361 4.1e-13 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015572)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11650
malus_domestica MD05G1261500.v1.1
prunus_persica Prupe.4G104000_v2.0.a1
pyrus_communis pycom05g24510 pycom10g20060
rosa_chinensis RchiOBHm_Chr5g0018711
rosa_laevigata RLG00000032389
rosa_multiflora Rmu_co8494637.1_g000001
rosa_roxburghii Rroxscaffold_1G00059070 Rroxscaffold_1G00073800
rosa_rugosa Rorug05G0046100
rosa_samantha Rh5AG137100 Rh5BG135900 Rh5CG147900 Rh5DG137000
rosa_wichuraiana Rw5G012150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1555
AatII GACGTC 2 cut(s) 324, 1073
AccB1I GGYRCC 2 cut(s) 488, 494
AccI GTMKAC 4 cut(s) 318, 324, 1014, 1041
AccII CGCG 9 cut(s) 632, 888, 890, 907, 909, 965, 974, 1076, 1290
AclWI GGATC 4 cut(s) 5, 657, 670, 1143
AcoI YGGCCR 5 cut(s) 513, 667, 671, 770, 1035
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 1536
AcvI CACGTG 2 cut(s) 71, 1090
AcyI GRCGYC 4 cut(s) 321, 489, 495, 1070
AfaI GTAC 4 cut(s) 86, 224, 237, 1478
AfiI CCNNNNNNNGG 2 cut(s) 853, 985
AflII CTTAAG 1 cut(s) 848
AflIII ACRYGT 6 cut(s) 68, 70, 876, 954, 1087, 1089
AgsI TTSAA 8 cut(s) 254, 298, 733, 943, 1096, 1251, 1462, 1543
AhdI GACNNNNNGTC 1 cut(s) 880
AjiI CACGTC 2 cut(s) 955, 969
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 7 cut(s) 181, 211, 215, 275, 933, 1223, 1244
AluI AGCT 7 cut(s) 181, 211, 215, 275, 933, 1223, 1244
Alw21I GWGCWC 4 cut(s) 47, 183, 962, 1454
Alw26I GTCTC 2 cut(s) 528, 744
Alw44I GTGCAC 1 cut(s) 958
AlwI GGATC 4 cut(s) 5, 657, 670, 1143
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 5 cut(s) 513, 667, 671, 770, 1035
ApaLI GTGCAC 1 cut(s) 958
ApeKI GCWGC 3 cut(s) 272, 983, 1197
ApoI RAATTY 1 cut(s) 254
ArsI GACNNNNNNTTYG 4 cut(s) 1507, 1522, 1539, 1554
Asp700I GAANNNNTTC 1 cut(s) 365
AspLEI GCGC 8 cut(s) 491, 497, 890, 892, 909, 911, 974, 976
AspS9I GGNCC 2 cut(s) 1272, 1333
AsuC2I CCSGG 2 cut(s) 980, 1270
AsuHPI GGTGA 6 cut(s) 8, 177, 448, 524, 833, 1501
AsuNHI GCTAGC 1 cut(s) 211
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 2 cut(s) 1272, 1333
BaeGI GKGCMC 2 cut(s) 697, 962
BamHI GGATCC 1 cut(s) 662
BanI GGYRCC 2 cut(s) 488, 494
BanII GRGCYC 1 cut(s) 183
BarI GAAGNNNNNNTAC 2 cut(s) 215, 247
BauI CACGAG 1 cut(s) 338
BbrPI CACGTG 2 cut(s) 71, 1090
BbsI GAAGAC 1 cut(s) 745
Bbv12I GWGCWC 4 cut(s) 47, 183, 962, 1454
BbvI GCAGC 3 cut(s) 284, 970, 1184
BccI CCATC 6 cut(s) 139, 148, 488, 653, 919, 1324
BceAI ACGGC 6 cut(s) 47, 502, 520, 563, 1022, 1060
BcgI CGANNNNNNTGC 2 cut(s) 1507, 1541
BclI TGATCA 2 cut(s) 1237, 1491
BcnI CCSGG 2 cut(s) 980, 1270
BcoDI GTCTC 2 cut(s) 528, 744
BfaI CTAG 2 cut(s) 212, 444
BfoI RGCGCY 2 cut(s) 492, 498
BfrI CTTAAG 1 cut(s) 848
BglII AGATCT 1 cut(s) 342
BisI GCNGC 9 cut(s) 270, 273, 516, 576, 633, 654, 984, 1077, 1198
BlsI GCNGC 9 cut(s) 271, 274, 517, 577, 634, 655, 985, 1078, 1199
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 2 cut(s) 980, 1270
Bme18I GGWCC 2 cut(s) 1272, 1333
BmeRI GACNNNNNGTC 1 cut(s) 880
BmeT110I CYCGRG 1 cut(s) 356
BmgBI CACGTC 2 cut(s) 955, 969
BmgT120I GGNCC 2 cut(s) 1272, 1333
BmiI GGNNCC 4 cut(s) 490, 496, 610, 664
BmrFI CCNGG 2 cut(s) 980, 1270
BmsI GCATC 2 cut(s) 555, 1314
BmtI GCTAGC 1 cut(s) 215
BpiI GAAGAC 1 cut(s) 745
BplI GAGNNNNNCTC 2 cut(s) 332, 364
BpmI CTGGAG 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 687
BpuEI CTTGAG 1 cut(s) 1222
BpuMI CCSGG 2 cut(s) 980, 1270
BsaAI YACGTR 2 cut(s) 71, 1090
BsaHI GRCGYC 4 cut(s) 321, 489, 495, 1070
BsaJI CCNNGG 1 cut(s) 1074
BsaWI WCCGGW 1 cut(s) 611
BsaXI ACNNNNNCTCC 8 cut(s) 290, 296, 320, 326, 406, 436, 992, 1022
Bsc4I CCNNNNNNNGG 2 cut(s) 853, 985
Bse118I RCCGGY 7 cut(s) 458, 491, 669, 673, 772, 1301, 1481
Bse1I ACTGG 2 cut(s) 220, 826
Bse3DI GCAATG 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 1074
BseGI GGATG 2 cut(s) 546, 1335
BseLI CCNNNNNNNGG 2 cut(s) 853, 985
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 3 cut(s) 168, 346, 701
BseNI ACTGG 2 cut(s) 220, 826
BsePI GCGCGC 3 cut(s) 888, 907, 972
BseRI GAGGAG 3 cut(s) 86, 414, 1522
BseSI GKGCMC 2 cut(s) 697, 962
BseX3I CGGCCG 3 cut(s) 667, 671, 770
BseXI GCAGC 3 cut(s) 284, 970, 1184
BsgI GTGCAG 1 cut(s) 1308
Bsh1236I CGCG 9 cut(s) 632, 888, 890, 907, 909, 965, 974, 1076, 1290
Bsh1285I CGRYCG 3 cut(s) 670, 674, 773
BshFI GGCC 5 cut(s) 515, 669, 673, 772, 1037
BshNI GGYRCC 2 cut(s) 488, 494
BsiEI CGRYCG 3 cut(s) 670, 674, 773
BsiHKAI GWGCWC 4 cut(s) 47, 183, 962, 1454
BsiHKCI CYCGRG 1 cut(s) 356
BsiWI CGTACG 1 cut(s) 235
BslFI GGGAC 1 cut(s) 868
BslI CCNNNNNNNGG 2 cut(s) 853, 985
BsmAI GTCTC 2 cut(s) 528, 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 868
BsnI GGCC 5 cut(s) 515, 669, 673, 772, 1037
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 5 cut(s) 47, 183, 697, 962, 1454
Bsp143I GATC 7 cut(s) 10, 342, 662, 1029, 1135, 1237, 1491
BspANI GGCC 5 cut(s) 515, 669, 673, 772, 1037
BspCNI CTCAG 3 cut(s) 169, 345, 700
BspFNI CGCG 9 cut(s) 632, 888, 890, 907, 909, 965, 974, 1076, 1290
BspLI GGNNCC 4 cut(s) 490, 496, 610, 664
BspOI GCTAGC 1 cut(s) 215
BspPI GGATC 4 cut(s) 5, 657, 670, 1143
BspQI GCTCTTC 1 cut(s) 809
BspT107I GGYRCC 2 cut(s) 488, 494
BspTI CTTAAG 1 cut(s) 848
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 7 cut(s) 458, 491, 669, 673, 772, 1301, 1481
BsrI ACTGG 2 cut(s) 220, 826
BssAI RCCGGY 7 cut(s) 458, 491, 669, 673, 772, 1301, 1481
BssECI CCNNGG 1 cut(s) 1074
BssHII GCGCGC 3 cut(s) 888, 907, 972
BssMI GATC 7 cut(s) 10, 342, 662, 1029, 1135, 1237, 1491
BssNI GRCGYC 4 cut(s) 321, 489, 495, 1070
BssSI CACGAG 1 cut(s) 338
Bst2BI CACGAG 1 cut(s) 338
Bst4CI ACNGT 3 cut(s) 240, 294, 1534
Bst6I CTCTTC 5 cut(s) 377, 432, 809, 1430, 1497
BstACI GRCGYC 4 cut(s) 321, 489, 495, 1070
BstAFI CTTAAG 1 cut(s) 848
BstBAI YACGTR 2 cut(s) 71, 1090
BstDEI CTNAG 3 cut(s) 177, 332, 687
BstDSI CCRYGG 1 cut(s) 1074
BstEII GGTNACC 1 cut(s) 821
BstF5I GGATG 2 cut(s) 546, 1335
BstFNI CGCG 9 cut(s) 632, 888, 890, 907, 909, 965, 974, 1076, 1290
BstH2I RGCGCY 2 cut(s) 492, 498
BstHHI GCGC 8 cut(s) 491, 497, 890, 892, 909, 911, 974, 976
BstKTI GATC 7 cut(s) 13, 345, 665, 1032, 1138, 1240, 1494
BstMAI GTCTC 2 cut(s) 528, 744
BstMBI GATC 7 cut(s) 10, 342, 662, 1029, 1135, 1237, 1491
BstMCI CGRYCG 3 cut(s) 670, 674, 773
BstMWI GCNNNNNNNGC 5 cut(s) 269, 692, 971, 980, 992
BstNSI RCATGY 1 cut(s) 880
BstPI GGTNACC 1 cut(s) 821
BstSCI CCNGG 2 cut(s) 978, 1268
BstSLI GKGCMC 2 cut(s) 697, 962
BstUI CGCG 9 cut(s) 632, 888, 890, 907, 909, 965, 974, 1076, 1290
BstV1I GCAGC 3 cut(s) 284, 970, 1184
BstV2I GAAGAC 1 cut(s) 745
BstX2I RGATCY 3 cut(s) 342, 662, 1135
BstXI CCANNNNNNTGG 1 cut(s) 26
BstYI RGATCY 3 cut(s) 342, 662, 1135
BstZI CGGCCG 3 cut(s) 667, 671, 770
BsuRI GGCC 5 cut(s) 515, 669, 673, 772, 1037
BtgI CCRYGG 1 cut(s) 1074
BtgZI GCGATG 1 cut(s) 1294
BtrI CACGTC 2 cut(s) 955, 969
BtsCI GGATG 2 cut(s) 546, 1335
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 3 cut(s) 265, 290, 681
CfoI GCGC 8 cut(s) 491, 497, 890, 892, 909, 911, 974, 976
Cfr10I RCCGGY 7 cut(s) 458, 491, 669, 673, 772, 1301, 1481
Cfr13I GGNCC 2 cut(s) 1272, 1333
Cfr42I CCGCGG 1 cut(s) 1077
CseI GACGC 1 cut(s) 923
Csp6I GTAC 4 cut(s) 85, 223, 236, 1477
CviAII CATG 4 cut(s) 436, 877, 1033, 1057
CviQI GTAC 4 cut(s) 85, 223, 236, 1477
DdeI CTNAG 3 cut(s) 177, 332, 687
DinI GGCGCC 2 cut(s) 490, 496
DpnI GATC 7 cut(s) 12, 344, 664, 1031, 1137, 1239, 1493
DpnII GATC 7 cut(s) 10, 342, 662, 1029, 1135, 1237, 1491
DrdI GACNNNNNNGTC 1 cut(s) 1555
DriI GACNNNNNGTC 1 cut(s) 880
DseDI GACNNNNNNGTC 1 cut(s) 1555
EaeI YGGCCR 5 cut(s) 513, 667, 671, 770, 1035
EagI CGGCCG 3 cut(s) 667, 671, 770
Eam1104I CTCTTC 5 cut(s) 377, 432, 809, 1430, 1497
Eam1105I GACNNNNNGTC 1 cut(s) 880
EarI CTCTTC 5 cut(s) 377, 432, 809, 1430, 1497
EciI GGCGGA 3 cut(s) 539, 797, 1062
Ecl136II GAGCTC 1 cut(s) 181
EclXI CGGCCG 3 cut(s) 667, 671, 770
Eco24I GRGCYC 1 cut(s) 183
Eco47I GGWCC 2 cut(s) 1272, 1333
Eco52I CGGCCG 3 cut(s) 667, 671, 770
Eco53kI GAGCTC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 1536
Eco72I CACGTG 2 cut(s) 71, 1090
Eco88I CYCGRG 1 cut(s) 356
Eco91I GGTNACC 1 cut(s) 821
EcoICRI GAGCTC 1 cut(s) 181
EcoO65I GGTNACC 1 cut(s) 821
EcoRI GAATTC 1 cut(s) 254
EcoT38I GRGCYC 1 cut(s) 183
EgeI GGCGCC 2 cut(s) 490, 496
EheI GGCGCC 2 cut(s) 490, 496
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 4 cut(s) 439, 880, 1036, 1060
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 1 cut(s) 868
FatI CATG 4 cut(s) 435, 876, 1032, 1056
FauI CCCGC 2 cut(s) 739, 893
FbaI TGATCA 2 cut(s) 1237, 1491
FblI GTMKAC 4 cut(s) 318, 324, 1014, 1041
Fnu4HI GCNGC 9 cut(s) 270, 273, 516, 576, 633, 654, 984, 1077, 1198
FokI GGATG 2 cut(s) 533, 1342
FriOI GRGCYC 1 cut(s) 183
FseI GGCCGGCC 1 cut(s) 673
Fsp4HI GCNGC 9 cut(s) 270, 273, 516, 576, 633, 654, 984, 1077, 1198
FspBI CTAG 2 cut(s) 212, 444
GlaI GCGC 8 cut(s) 490, 496, 889, 891, 908, 910, 973, 975
GluI GCNGC 9 cut(s) 270, 273, 516, 576, 633, 654, 984, 1077, 1198
GsuI CTGGAG 1 cut(s) 203
HaeII RGCGCY 2 cut(s) 492, 498
HaeIII GGCC 5 cut(s) 515, 669, 673, 772, 1037
HgaI GACGC 1 cut(s) 923
HhaI GCGC 8 cut(s) 491, 497, 890, 892, 909, 911, 974, 976
Hin1I GRCGYC 4 cut(s) 321, 489, 495, 1070
Hin1II CATG 4 cut(s) 439, 880, 1036, 1060
Hin6I GCGC 8 cut(s) 489, 495, 888, 890, 907, 909, 972, 974
HinP1I GCGC 8 cut(s) 489, 495, 888, 890, 907, 909, 972, 974
HincII GTYRAC 5 cut(s) 319, 325, 831, 1015, 1042
HindII GTYRAC 5 cut(s) 319, 325, 831, 1015, 1042
HphI GGTGA 6 cut(s) 8, 177, 448, 524, 833, 1501
Hpy188I TCNGA 8 cut(s) 178, 310, 586, 1068, 1157, 1171, 1178, 1228
Hpy188III TCNNGA 3 cut(s) 356, 358, 710
HpyAV CCTTC 7 cut(s) 716, 772, 781, 832, 943, 1200, 1211
HpyCH4III ACNGT 3 cut(s) 240, 294, 1534
HpyCH4IV ACGT 8 cut(s) 70, 321, 597, 815, 954, 968, 1070, 1089
HpyCH4V TGCA 5 cut(s) 263, 546, 960, 1294, 1325
HpyF10VI GCNNNNNNNGC 5 cut(s) 269, 692, 971, 980, 992
HpyF3I CTNAG 3 cut(s) 177, 332, 687
HpySE526I ACGT 8 cut(s) 70, 321, 597, 815, 954, 968, 1070, 1089
Hsp92I GRCGYC 4 cut(s) 321, 489, 495, 1070
Hsp92II CATG 4 cut(s) 439, 880, 1036, 1060
HspAI GCGC 8 cut(s) 489, 495, 888, 890, 907, 909, 972, 974
KasI GGCGCC 2 cut(s) 488, 494
KroI GCCGGC 4 cut(s) 491, 669, 772, 1481
KroNI GCCGGC 4 cut(s) 493, 671, 774, 1483
Ksp22I TGATCA 2 cut(s) 1237, 1491
KspI CCGCGG 1 cut(s) 1077
Kzo9I GATC 7 cut(s) 10, 342, 662, 1029, 1135, 1237, 1491
LguI GCTCTTC 1 cut(s) 809
LmnI GCTCC 1 cut(s) 1220
Lsp1109I GCAGC 3 cut(s) 284, 970, 1184
LweI GCATC 2 cut(s) 555, 1314
MaeI CTAG 2 cut(s) 212, 444
MaeII ACGT 8 cut(s) 70, 321, 597, 815, 954, 968, 1070, 1089
MaeIII GTNAC 4 cut(s) 64, 73, 821, 1149
MalI GATC 7 cut(s) 12, 344, 664, 1031, 1137, 1239, 1493
MboI GATC 7 cut(s) 10, 342, 662, 1029, 1135, 1237, 1491
MflI RGATCY 3 cut(s) 342, 662, 1135
MhlI GDGCHC 5 cut(s) 47, 183, 697, 962, 1454
MluCI AATT 4 cut(s) 243, 254, 1050, 1279
Mly113I GGCGCC 2 cut(s) 489, 495
MlyI GAGTC 7 cut(s) 165, 299, 305, 560, 1175, 1424, 1603
MmeI TCCRAC 7 cut(s) 177, 333, 1091, 1149, 1251, 1365, 1449
MreI CGCCGGCG 2 cut(s) 491, 1481
MroNI GCCGGC 4 cut(s) 491, 669, 772, 1481
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 4 cut(s) 242, 476, 849, 1005
MslI CAYNNNNRTG 2 cut(s) 71, 1606
MspA1I CMGCKG 1 cut(s) 1076
MspCI CTTAAG 1 cut(s) 848
MspR9I CCNGG 2 cut(s) 980, 1270
MvnI CGCG 9 cut(s) 632, 888, 890, 907, 909, 965, 974, 1076, 1290
MwoI GCNNNNNNNGC 5 cut(s) 269, 692, 971, 980, 992
NaeI GCCGGC 4 cut(s) 493, 671, 774, 1483
NarI GGCGCC 2 cut(s) 489, 495
NciI CCSGG 2 cut(s) 980, 1270
NdeII GATC 7 cut(s) 10, 342, 662, 1029, 1135, 1237, 1491
NgoMIV GCCGGC 4 cut(s) 491, 669, 772, 1481
NheI GCTAGC 1 cut(s) 211
NlaIII CATG 4 cut(s) 439, 880, 1036, 1060
NlaIV GGNNCC 4 cut(s) 490, 496, 610, 664
NmuCI GTSAC 4 cut(s) 64, 73, 821, 1149
NspI RCATGY 1 cut(s) 880
OliI CACNNNNGTG 1 cut(s) 71
PaeR7I CTCGAG 1 cut(s) 356
PauI GCGCGC 3 cut(s) 888, 907, 972
PciI ACATGT 1 cut(s) 876
PciSI GCTCTTC 1 cut(s) 809
PcsI WCGNNNNNNNCGW 1 cut(s) 534
PdiI GCCGGC 4 cut(s) 493, 671, 774, 1483
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 4 cut(s) 471, 539, 862, 1206
Pfl23II CGTACG 1 cut(s) 235
PflFI GACNNNGTC 1 cut(s) 314
PfoI TCCNGGA 1 cut(s) 1268
PkrI GCNGC 9 cut(s) 271, 274, 517, 577, 634, 655, 985, 1078, 1199
PleI GAGTC 7 cut(s) 165, 299, 305, 560, 1174, 1423, 1602
PluTI GGCGCC 2 cut(s) 492, 498
PmaCI CACGTG 2 cut(s) 71, 1090
PmlI CACGTG 2 cut(s) 71, 1090
PpsI GAGTC 7 cut(s) 165, 299, 305, 560, 1174, 1423, 1602
Ppu21I YACGTR 2 cut(s) 71, 1090
PscI ACATGT 1 cut(s) 876
Psp124BI GAGCTC 1 cut(s) 183
PspCI CACGTG 2 cut(s) 71, 1090
PspEI GGTNACC 1 cut(s) 821
PspLI CGTACG 1 cut(s) 235
PspN4I GGNNCC 4 cut(s) 490, 496, 610, 664
PspPI GGNCC 2 cut(s) 1272, 1333
PsuI RGATCY 3 cut(s) 342, 662, 1135
PsyI GACNNNGTC 1 cut(s) 314
PteI GCGCGC 3 cut(s) 888, 907, 972
RigI GGCCGGCC 1 cut(s) 673
RsaI GTAC 4 cut(s) 86, 224, 237, 1478
RsaNI GTAC 4 cut(s) 85, 223, 236, 1477
RseI CAYNNNNRTG 2 cut(s) 71, 1606
SacI GAGCTC 1 cut(s) 183
SacII CCGCGG 1 cut(s) 1077
SalI GTCGAC 4 cut(s) 317, 323, 1013, 1040
SapI GCTCTTC 1 cut(s) 809
SaqAI TTAA 4 cut(s) 242, 476, 849, 1005
SatI GCNGC 9 cut(s) 270, 273, 516, 576, 633, 654, 984, 1077, 1198
Sau3AI GATC 7 cut(s) 10, 342, 662, 1029, 1135, 1237, 1491
Sau96I GGNCC 2 cut(s) 1272, 1333
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 7 cut(s) 165, 299, 305, 560, 1175, 1424, 1603
ScrFI CCNGG 2 cut(s) 980, 1270
SduI GDGCHC 5 cut(s) 47, 183, 697, 962, 1454
SfaNI GCATC 2 cut(s) 555, 1314
SfoI GGCGCC 2 cut(s) 490, 496
Sfr274I CTCGAG 1 cut(s) 356
Sfr303I CCGCGG 1 cut(s) 1077
SgrAI CRCCGGYG 2 cut(s) 491, 1481
SgrBI CCGCGG 1 cut(s) 1077
SgrDI CGTCGACG 2 cut(s) 317, 1040
SinI GGWCC 2 cut(s) 1272, 1333
SlaI CTCGAG 1 cut(s) 356
SmiMI CAYNNNNRTG 2 cut(s) 71, 1606
SmlI CTYRAG 3 cut(s) 356, 848, 1201
SmoI CTYRAG 3 cut(s) 356, 848, 1201
Sse9I AATT 4 cut(s) 243, 254, 1050, 1279
SspDI GGCGCC 2 cut(s) 488, 494
SspMI CTAG 2 cut(s) 212, 444
SstI GAGCTC 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 978, 1268
TaaI ACNGT 3 cut(s) 240, 294, 1534
TaiI ACGT 8 cut(s) 73, 324, 600, 818, 957, 971, 1073, 1092
TasI AATT 4 cut(s) 243, 254, 1050, 1279
TatI WGTACW 2 cut(s) 84, 222
TauI GCSGC 6 cut(s) 272, 518, 578, 635, 656, 1079
TfiI GAWTC 4 cut(s) 471, 539, 862, 1206
Tru1I TTAA 4 cut(s) 242, 476, 849, 1005
Tru9I TTAA 4 cut(s) 242, 476, 849, 1005
TscAI CASTG 3 cut(s) 265, 297, 688
TseFI GTSAC 4 cut(s) 64, 73, 821, 1149
TseI GCWGC 3 cut(s) 272, 983, 1197
Tsp45I GTSAC 4 cut(s) 64, 73, 821, 1149
TspDTI ATGAA 5 cut(s) 419, 452, 851, 962, 1073
TspGWI ACGGA 2 cut(s) 304, 1342
TspRI CASTG 3 cut(s) 265, 297, 688
Tth111I GACNNNGTC 1 cut(s) 314
Vha464I CTTAAG 1 cut(s) 848
VneI GTGCAC 1 cut(s) 958
VpaK11BI GGWCC 2 cut(s) 1272, 1333
XapI RAATTY 1 cut(s) 254
XceI RCATGY 1 cut(s) 880
XhoI CTCGAG 1 cut(s) 356
XmiI GTMKAC 4 cut(s) 318, 324, 1014, 1041
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 2 cut(s) 212, 444
ZraI GACGTC 2 cut(s) 322, 1071
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.