Rroxscaffold_1G00073800

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015572
Physical Location & Seq
Forward (+)
94553687 .. 94555356
1670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073800.1

Sequence Viewer

Length: 687 bp
ATGGACGGCTACGATTTCAAGATGAAGGACGTGTGCACCGCCACTTGCGCGCATCCGGCGGCGAAGGGGGCCGTGGAGGTTAAGTCAGTCGACGGGAGGACGAAGATCATGGCCGTCGACGGCGGAATTGCCATGAACAATCCGACGGCCGCGGCTATTACGCACGTGTTGAATAATAAGCAAGAGTTTCCGTTCTGTTCTGGTGTGGAGGATCTCTTGGTGGTGTCACTCGGAAATGGAGAGTCGGAGTTCGGAAACTTGTCGCCTTCTGGCTGCCTAAGAATCGCCGGCGAAGGAGCGTCAGACTTGGTTGATCAAGCTGTTTCAATGGCATTTGGGCAATCCCGGACCAGCAATTATGTTCGCGTGCAAGCCACCGGCATTATAGCCAAAAACACTGCGCGCATGGACCACTCCAACAACAAGAACAACACCAATACAGACATATTGGCTTTAACAGAGGAAATGTTGGCACAGAGAAACGTGGAGTCGATTTTATTCGAGGGGAAGAAAATGGTGGAGAGCACGAATTTGAAGAAGTTGGAGTTGTTCGCCGGAGAGGTGATCAAGGAAGAAGAGAGGAGAAAGACCAGCATTTTGCCGACGGTGGCTTTGAAGCAGACTTCGGGGTCGCCAGCCAGAACATCGTCTGCTACTACCCTATCAAGTCTGTCATCTTCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

228

Amino Acids

24.24

Weight (kDa)

5.98

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015572)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11650
malus_domestica MD05G1261500.v1.1
prunus_persica Prupe.4G104000_v2.0.a1
pyrus_communis pycom05g24510 pycom10g20060
rosa_chinensis RchiOBHm_Chr5g0018711
rosa_laevigata RLG00000032389
rosa_multiflora Rmu_co8494637.1_g000001
rosa_roxburghii Rroxscaffold_1G00059070 Rroxscaffold_1G00073800
rosa_rugosa Rorug05G0046100
rosa_samantha Rh5AG137100 Rh5BG135900 Rh5CG147900 Rh5DG137000
rosa_wichuraiana Rw5G012150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 628
AccI GTMKAC 2 cut(s) 90, 117
AccII CGCG 4 cut(s) 50, 152, 366, 403
AciI CCGC 5 cut(s) 39, 59, 123, 150, 152
AclWI GGATC 1 cut(s) 219
AcoI YGGCCR 2 cut(s) 111, 147
AcsI RAATTY 1 cut(s) 529
AcvI CACGTG 1 cut(s) 166
AflIII ACRYGT 2 cut(s) 30, 165
AgsI TTSAA 5 cut(s) 19, 172, 327, 535, 616
AjiI CACGTC 1 cut(s) 31
AluBI AGCT 1 cut(s) 320
AluI AGCT 1 cut(s) 320
Alw21I GWGCWC 2 cut(s) 38, 527
Alw44I GTGCAC 1 cut(s) 34
AlwI GGATC 1 cut(s) 219
AoxI GGCC 3 cut(s) 69, 111, 147
ApaLI GTGCAC 1 cut(s) 34
ApeKI GCWGC 1 cut(s) 273
ApoI RAATTY 1 cut(s) 529
ArsI GACNNNNNNTTYG 4 cut(s) 580, 595, 612, 627
AspLEI GCGC 4 cut(s) 50, 52, 403, 405
AspS9I GGNCC 3 cut(s) 69, 348, 409
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 1 cut(s) 574
AvaII GGWCC 2 cut(s) 348, 409
BaeGI GKGCMC 1 cut(s) 38
BbrPI CACGTG 1 cut(s) 166
Bbv12I GWGCWC 2 cut(s) 38, 527
BbvI GCAGC 1 cut(s) 260
BceAI ACGGC 5 cut(s) 22, 56, 98, 136, 162
BclI TGATCA 2 cut(s) 313, 564
BcnI CCSGG 1 cut(s) 346
BisI GCNGC 4 cut(s) 60, 150, 153, 274
BlsI GCNGC 4 cut(s) 61, 151, 154, 275
Bme1390I CCNGG 1 cut(s) 346
Bme18I GGWCC 2 cut(s) 348, 409
BmgBI CACGTC 1 cut(s) 31
BmgT120I GGNCC 3 cut(s) 69, 348, 409
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 1 cut(s) 346
BmsI GCATC 1 cut(s) 61
BpuMI CCSGG 1 cut(s) 346
BsaAI YACGTR 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 72, 150
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bse118I RCCGGY 2 cut(s) 287, 377
BseDI CCNNGG 2 cut(s) 72, 150
BseGI GGATG 1 cut(s) 52
BsePI GCGCGC 2 cut(s) 48, 401
BseRI GAGGAG 1 cut(s) 595
BseSI GKGCMC 1 cut(s) 38
BseX3I CGGCCG 1 cut(s) 147
BseXI GCAGC 1 cut(s) 260
Bsh1236I CGCG 4 cut(s) 50, 152, 366, 403
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 3 cut(s) 71, 113, 149
BsiEI CGRYCG 1 cut(s) 150
BsiHKAI GWGCWC 2 cut(s) 38, 527
BsiSI CCGG 5 cut(s) 56, 288, 346, 378, 555
BsnI GGCC 3 cut(s) 71, 113, 149
Bsp1286I GDGCHC 2 cut(s) 38, 527
Bsp143I GATC 4 cut(s) 105, 211, 313, 564
BspACI CCGC 5 cut(s) 39, 59, 123, 150, 152
BspANI GGCC 3 cut(s) 71, 113, 149
BspFNI CGCG 4 cut(s) 50, 152, 366, 403
BspLI GGNNCC 1 cut(s) 70
BspPI GGATC 1 cut(s) 219
BsrFI RCCGGY 2 cut(s) 287, 377
BssAI RCCGGY 2 cut(s) 287, 377
BssECI CCNNGG 2 cut(s) 72, 150
BssHII GCGCGC 2 cut(s) 48, 401
BssMI GATC 4 cut(s) 105, 211, 313, 564
Bst4CI ACNGT 1 cut(s) 607
Bst6I CTCTTC 1 cut(s) 570
BstBAI YACGTR 1 cut(s) 166
BstC8I GCNNGC 6 cut(s) 50, 289, 368, 372, 403, 636
BstDEI CTNAG 1 cut(s) 278
BstDSI CCRYGG 2 cut(s) 72, 150
BstF5I GGATG 1 cut(s) 52
BstFNI CGCG 4 cut(s) 50, 152, 366, 403
BstHHI GCGC 4 cut(s) 50, 52, 403, 405
BstKTI GATC 4 cut(s) 108, 214, 316, 567
BstMBI GATC 4 cut(s) 105, 211, 313, 564
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 3 cut(s) 47, 56, 68
BstSCI CCNGG 1 cut(s) 344
BstSLI GKGCMC 1 cut(s) 38
BstUI CGCG 4 cut(s) 50, 152, 366, 403
BstV1I GCAGC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BstZI CGGCCG 1 cut(s) 147
BsuRI GGCC 3 cut(s) 71, 113, 149
BtgI CCRYGG 2 cut(s) 72, 150
BtrI CACGTC 1 cut(s) 31
BtsCI GGATG 1 cut(s) 52
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 1 cut(s) 396
Cac8I GCNNGC 6 cut(s) 50, 289, 368, 372, 403, 636
CfoI GCGC 4 cut(s) 50, 52, 403, 405
Cfr10I RCCGGY 2 cut(s) 287, 377
Cfr13I GGNCC 3 cut(s) 69, 348, 409
Cfr42I CCGCGG 1 cut(s) 153
CseI GACGC 1 cut(s) 288
CviAII CATG 3 cut(s) 109, 133, 406
DdeI CTNAG 1 cut(s) 278
DpnI GATC 4 cut(s) 107, 213, 315, 566
DpnII GATC 4 cut(s) 105, 211, 313, 564
DrdI GACNNNNNNGTC 1 cut(s) 628
DseDI GACNNNNNNGTC 1 cut(s) 628
EaeI YGGCCR 2 cut(s) 111, 147
EagI CGGCCG 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
EciI GGCGGA 1 cut(s) 138
EclXI CGGCCG 1 cut(s) 147
Eco47I GGWCC 2 cut(s) 348, 409
Eco52I CGGCCG 1 cut(s) 147
Eco72I CACGTG 1 cut(s) 166
FaeI CATG 3 cut(s) 112, 136, 409
FaiI YATR 6 cut(s) 110, 134, 360, 386, 407, 446
FatI CATG 3 cut(s) 108, 132, 405
FbaI TGATCA 2 cut(s) 313, 564
FblI GTMKAC 2 cut(s) 90, 117
Fnu4HI GCNGC 4 cut(s) 60, 150, 153, 274
FokI GGATG 1 cut(s) 39
Fsp4HI GCNGC 4 cut(s) 60, 150, 153, 274
GlaI GCGC 4 cut(s) 49, 51, 402, 404
GluI GCNGC 4 cut(s) 60, 150, 153, 274
HaeIII GGCC 3 cut(s) 71, 113, 149
HapII CCGG 5 cut(s) 56, 288, 346, 378, 555
HgaI GACGC 1 cut(s) 288
HhaI GCGC 4 cut(s) 50, 52, 403, 405
Hin1II CATG 3 cut(s) 112, 136, 409
Hin6I GCGC 4 cut(s) 48, 50, 401, 403
HinP1I GCGC 4 cut(s) 48, 50, 401, 403
HincII GTYRAC 2 cut(s) 91, 118
HindII GTYRAC 2 cut(s) 91, 118
HinfI GANTC 3 cut(s) 242, 282, 488
HpaII CCGG 5 cut(s) 56, 288, 346, 378, 555
HphI GGTGA 1 cut(s) 574
Hpy166II GTNNAC 3 cut(s) 36, 91, 118
Hpy188I TCNGA 5 cut(s) 144, 233, 247, 254, 304
Hpy188III TCNNGA 1 cut(s) 19
Hpy8I GTNNAC 3 cut(s) 36, 91, 118
Hpy99I CGWCG 5 cut(s) 95, 119, 122, 148, 607
HpyAV CCTTC 4 cut(s) 19, 58, 276, 287
HpyCH4III ACNGT 1 cut(s) 607
HpyCH4IV ACGT 3 cut(s) 30, 165, 483
HpyCH4V TGCA 2 cut(s) 36, 370
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 56, 68
HpyF3I CTNAG 1 cut(s) 278
HpySE526I ACGT 3 cut(s) 30, 165, 483
Hsp92II CATG 3 cut(s) 112, 136, 409
HspAI GCGC 4 cut(s) 48, 50, 401, 403
KroI GCCGGC 1 cut(s) 287
KroNI GCCGGC 1 cut(s) 289
Ksp22I TGATCA 2 cut(s) 313, 564
KspI CCGCGG 1 cut(s) 153
Kzo9I GATC 4 cut(s) 105, 211, 313, 564
LmnI GCTCC 1 cut(s) 296
Lsp1109I GCAGC 1 cut(s) 260
LweI GCATC 1 cut(s) 61
MaeII ACGT 3 cut(s) 30, 165, 483
MaeIII GTNAC 1 cut(s) 225
MalI GATC 4 cut(s) 107, 213, 315, 566
MboI GATC 4 cut(s) 105, 211, 313, 564
MboII GAAGA 6 cut(s) 115, 520, 547, 584, 587, 669
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 2 cut(s) 38, 527
MluCI AATT 3 cut(s) 126, 355, 529
MlyI GAGTC 2 cut(s) 251, 497
MmeI TCCRAC 4 cut(s) 167, 225, 441, 522
MnlI CCTC 7 cut(s) 70, 90, 202, 454, 496, 553, 573
MreI CGCCGGCG 1 cut(s) 287
MroNI GCCGGC 1 cut(s) 287
MseI TTAA 2 cut(s) 81, 455
MslI CAYNNNNRTG 1 cut(s) 679
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 5 cut(s) 56, 288, 346, 378, 555
MspR9I CCNGG 1 cut(s) 346
MvnI CGCG 4 cut(s) 50, 152, 366, 403
MwoI GCNNNNNNNGC 3 cut(s) 47, 56, 68
NaeI GCCGGC 1 cut(s) 289
NciI CCSGG 1 cut(s) 346
NdeII GATC 4 cut(s) 105, 211, 313, 564
NgoMIV GCCGGC 1 cut(s) 287
NlaIII CATG 3 cut(s) 112, 136, 409
NlaIV GGNNCC 1 cut(s) 70
NmuCI GTSAC 1 cut(s) 225
PauI GCGCGC 2 cut(s) 48, 401
PdiI GCCGGC 1 cut(s) 289
PfeI GAWTC 1 cut(s) 282
PfoI TCCNGGA 1 cut(s) 344
PkrI GCNGC 4 cut(s) 61, 151, 154, 275
PleI GAGTC 2 cut(s) 250, 496
PmaCI CACGTG 1 cut(s) 166
PmlI CACGTG 1 cut(s) 166
PpsI GAGTC 2 cut(s) 250, 496
Ppu21I YACGTR 1 cut(s) 166
PspCI CACGTG 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 3 cut(s) 69, 348, 409
PsuI RGATCY 1 cut(s) 211
PteI GCGCGC 2 cut(s) 48, 401
RseI CAYNNNNRTG 1 cut(s) 679
SacII CCGCGG 1 cut(s) 153
SalI GTCGAC 2 cut(s) 89, 116
SaqAI TTAA 2 cut(s) 81, 455
SatI GCNGC 4 cut(s) 60, 150, 153, 274
Sau3AI GATC 4 cut(s) 105, 211, 313, 564
Sau96I GGNCC 3 cut(s) 69, 348, 409
SchI GAGTC 2 cut(s) 251, 497
ScrFI CCNGG 1 cut(s) 346
SduI GDGCHC 2 cut(s) 38, 527
SetI ASST 6 cut(s) 33, 81, 168, 322, 486, 564
SfaNI GCATC 1 cut(s) 61
Sfr303I CCGCGG 1 cut(s) 153
SgrAI CRCCGGYG 1 cut(s) 287
SgrBI CCGCGG 1 cut(s) 153
SgrDI CGTCGACG 1 cut(s) 116
SinI GGWCC 2 cut(s) 348, 409
SmiMI CAYNNNNRTG 1 cut(s) 679
Sse9I AATT 3 cut(s) 126, 355, 529
SsiI CCGC 5 cut(s) 39, 59, 123, 150, 152
StyD4I CCNGG 1 cut(s) 344
TaaI ACNGT 1 cut(s) 607
TaiI ACGT 3 cut(s) 33, 168, 486
TaqI TCGA 4 cut(s) 90, 117, 491, 501
TasI AATT 3 cut(s) 126, 355, 529
TauI GCSGC 3 cut(s) 62, 152, 155
TfiI GAWTC 1 cut(s) 282
Tru1I TTAA 2 cut(s) 81, 455
Tru9I TTAA 2 cut(s) 81, 455
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 1 cut(s) 273
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 2 cut(s) 38, 149
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 1 cut(s) 403
VneI GTGCAC 1 cut(s) 34
VpaK11BI GGWCC 2 cut(s) 348, 409
XapI RAATTY 1 cut(s) 529
XmiI GTMKAC 2 cut(s) 90, 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.