Rh5DG137000

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0015572
Physical Location & Seq
Forward (+)
13758970 .. 13761978
3009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG137000.1

Sequence Viewer

Length: 1347 bp
ATGGCGGCAGCAGCAGCGCTATCCACATCTCCACTGTTCAACATAGACTCGAACTCCGTCGACGTTGACAAGCTCAGCCACGAGATCTTCTCCATTCTCGAGAACAAGTTCCTTTTCGGTTACGACATTGATAACTCCAAAGACACTACTGCTACCACGCCTCTTCATCACATGAAGTCTAGCAAGCACATCACCGGCAAGGTCAGAATCTTAACCATCGACGGCGCCGGAGCCACCGACGGGGTTTTGGCAGCCAAGTCTCTCGTCCACCTCGAGTCCTGCATCCGCCGCAAGTCCGGCGACTCCACTGCCGCCGTTTCTGACTACTTTGATGTTGTTTCTGGCTCCGGCGTCGGCGGCATTCTCGCGGCTCTGCTCTTCACCAAGGCAGCCGACGGATCGGGCCGGCCGGTGTTCACTGCTCAGGAGGCACTCAACTTCCTTTTGGAAAACCGAAGTAAGATTTTCAAGTCGTCTACGGCAGGAATATTCCGGCGAGTTTTCCGGCCGGCGAAGGCTGAGAAGGAGAGGGAGAGGCTCTTCCGCAAGACGTTCGGCGATCAGCTGACGATGAAGGACACCCTTAAGTCGGTGTTGATTCCCTGCTACGACATGTCGTCCCGCGCGCCTTTTCTGTTTTCGCGCGCCGATGCTGTGGAGATGGACGGCTACGATTTCAAGATGAAGGACGTGTGCACCGCCACGTGCGCGCATCCGGCGGCGACGGGGGCCGTGGAGGTTAAGTCAATCGACGGGAGGACGAAGATCATGGCCGTCGACGGCGGAATTGCCATGAACAATCCGACGGCCGCCGCTATTACGCACGTTCTGAATAATAAGCAAGAGTTTCCGTTCTGTTCTGGTGTGGAGGATCTCTTGGTGGTGTCACTCGGAAATGGAGAGTCGGAGTTCGGAAACTTGTCGCCTTCTGGCTGCCTAAGAATCGCCGGCGAAGGAGCTTCAGACTTGGTTGATCAAGCTGTTTCAATGGCATTTGGGCAATCCCGGACCAGCAATTATGTTCGCGTGCAAGCCACCGGCATCATAGCCAAAAACCCTGCGCGCATGGACCACTCCAACAACAAGAACAACACCAATACAGACATATTGGCTTTAACAGAGGAAATGTTGGCACAGAGAAACGTGGAGTCGATTTTATTCGAGGGGAAGAAAATGGTGGAGAGCACGAATTTGAAGAAGTTGGAGTTGTTCGCCGGAGAGGTGATCAAGGAAGAAGAGAGGAGAAAGACCAGCATTTTGCCGACGGTGGCTTTGAAGCAGACTTCGGGGTCGCCAGCCAGAACATCGTCTGCTACTACCTTATCGAGTCTGTCATCTTCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

448

Amino Acids

48.08

Weight (kDa)

7.6

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 74 - 273 7.4e-14 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015572)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11650
malus_domestica MD05G1261500.v1.1
prunus_persica Prupe.4G104000_v2.0.a1
pyrus_communis pycom05g24510 pycom10g20060
rosa_chinensis RchiOBHm_Chr5g0018711
rosa_laevigata RLG00000032389
rosa_multiflora Rmu_co8494637.1_g000001
rosa_roxburghii Rroxscaffold_1G00059070 Rroxscaffold_1G00073800
rosa_rugosa Rorug05G0046100
rosa_samantha Rh5AG137100 Rh5BG135900 Rh5CG147900 Rh5DG137000
rosa_wichuraiana Rw5G012150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1288
AccB1I GGYRCC 1 cut(s) 224
AccI GTMKAC 3 cut(s) 60, 476, 777
AccII CGCG 8 cut(s) 368, 624, 626, 643, 645, 710, 1026, 1063
AclWI GGATC 2 cut(s) 406, 879
AcoI YGGCCR 4 cut(s) 407, 506, 771, 807
AcsI RAATTY 1 cut(s) 1189
AcuI CTGAAG 1 cut(s) 945
AcvI CACGTG 1 cut(s) 705
AcyI GRCGYC 2 cut(s) 225, 351
AfeI AGCGCT 1 cut(s) 18
AfiI CCNNNNNNNGG 2 cut(s) 240, 589
AflII CTTAAG 1 cut(s) 584
AflIII ACRYGT 2 cut(s) 612, 690
AgsI TTSAA 6 cut(s) 40, 469, 679, 987, 1195, 1276
AhdI GACNNNNNGTC 1 cut(s) 616
AjiI CACGTC 1 cut(s) 691
AluBI AGCT 4 cut(s) 73, 565, 959, 980
AluI AGCT 4 cut(s) 73, 565, 959, 980
Alw21I GWGCWC 2 cut(s) 698, 1187
Alw26I GTCTC 1 cut(s) 264
Alw44I GTGCAC 1 cut(s) 694
AlwI GGATC 2 cut(s) 406, 879
Ama87I CYCGRG 2 cut(s) 98, 272
Aor51HI AGCGCT 1 cut(s) 18
AoxI GGCC 6 cut(s) 403, 407, 506, 729, 771, 807
ApaLI GTGCAC 1 cut(s) 694
ApeKI GCWGC 6 cut(s) 8, 11, 14, 251, 389, 933
ApoI RAATTY 1 cut(s) 1189
ArsI GACNNNNNNTTYG 4 cut(s) 1240, 1255, 1272, 1287
Asp700I GAANNNNTTC 1 cut(s) 107
AspS9I GGNCC 4 cut(s) 403, 729, 1008, 1069
AsuC2I CCSGG 1 cut(s) 1006
AsuHPI GGTGA 3 cut(s) 184, 373, 1234
AvaI CYCGRG 2 cut(s) 98, 272
AvaII GGWCC 2 cut(s) 1008, 1069
BaeGI GKGCMC 1 cut(s) 698
BanI GGYRCC 1 cut(s) 224
BauI CACGAG 1 cut(s) 80
BbrPI CACGTG 1 cut(s) 705
Bbv12I GWGCWC 2 cut(s) 698, 1187
BbvI GCAGC 6 cut(s) 20, 23, 26, 263, 401, 920
BccI CCATC 2 cut(s) 224, 655
BceAI ACGGC 8 cut(s) 238, 299, 495, 682, 716, 758, 796, 822
BcgI CGANNNNNNTGC 4 cut(s) 290, 324, 535, 569
BclI TGATCA 2 cut(s) 973, 1224
BcnI CCSGG 1 cut(s) 1006
BcoDI GTCTC 1 cut(s) 264
BfaI CTAG 1 cut(s) 180
BfoI RGCGCY 2 cut(s) 20, 228
BfrI CTTAAG 1 cut(s) 584
BglII AGATCT 1 cut(s) 84
BlpI GCTNAGC 1 cut(s) 74
Bme1390I CCNGG 1 cut(s) 1006
Bme18I GGWCC 2 cut(s) 1008, 1069
BmeRI GACNNNNNGTC 1 cut(s) 616
BmeT110I CYCGRG 2 cut(s) 98, 272
BmgBI CACGTC 1 cut(s) 691
BmgT120I GGNCC 4 cut(s) 403, 729, 1008, 1069
BmiI GGNNCC 4 cut(s) 226, 232, 346, 730
BmrFI CCNGG 1 cut(s) 1006
BmsI GCATC 4 cut(s) 291, 640, 721, 1050
BplI GAGNNNNNCTC 2 cut(s) 74, 106
Bpu10I CCTNAGC 1 cut(s) 423
Bpu1102I GCTNAGC 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 1006
BsaAI YACGTR 1 cut(s) 705
BsaHI GRCGYC 2 cut(s) 225, 351
BsaJI CCNNGG 2 cut(s) 384, 732
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 728, 758
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 589
Bse118I RCCGGY 6 cut(s) 194, 405, 409, 508, 947, 1037
BseDI CCNNGG 2 cut(s) 384, 732
BseGI GGATG 2 cut(s) 282, 712
BseLI CCNNNNNNNGG 2 cut(s) 240, 589
BseMII CTCAG 3 cut(s) 88, 437, 510
BsePI GCGCGC 4 cut(s) 624, 643, 708, 1061
BseRI GAGGAG 1 cut(s) 1255
BseSI GKGCMC 1 cut(s) 698
BseX3I CGGCCG 3 cut(s) 407, 506, 807
BseXI GCAGC 6 cut(s) 20, 23, 26, 263, 401, 920
Bsh1236I CGCG 8 cut(s) 368, 624, 626, 643, 645, 710, 1026, 1063
Bsh1285I CGRYCG 3 cut(s) 410, 509, 810
BshFI GGCC 6 cut(s) 405, 409, 508, 731, 773, 809
BshNI GGYRCC 1 cut(s) 224
BsiEI CGRYCG 3 cut(s) 410, 509, 810
BsiHKAI GWGCWC 2 cut(s) 698, 1187
BsiHKCI CYCGRG 2 cut(s) 98, 272
BslFI GGGAC 1 cut(s) 604
BslI CCNNNNNNNGG 2 cut(s) 240, 589
BsmAI GTCTC 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 604
BsmI GAATGC 1 cut(s) 360
BsnI GGCC 6 cut(s) 405, 409, 508, 731, 773, 809
BsoBI CYCGRG 2 cut(s) 98, 272
Bsp1286I GDGCHC 2 cut(s) 698, 1187
Bsp143I GATC 7 cut(s) 84, 398, 559, 765, 871, 973, 1224
Bsp1720I GCTNAGC 1 cut(s) 74
BspANI GGCC 6 cut(s) 405, 409, 508, 731, 773, 809
BspCNI CTCAG 3 cut(s) 87, 436, 511
BspFNI CGCG 8 cut(s) 368, 624, 626, 643, 645, 710, 1026, 1063
BspLI GGNNCC 4 cut(s) 226, 232, 346, 730
BspPI GGATC 2 cut(s) 406, 879
BspQI GCTCTTC 2 cut(s) 383, 545
BspT107I GGYRCC 1 cut(s) 224
BspTI CTTAAG 1 cut(s) 584
BsrFI RCCGGY 6 cut(s) 194, 405, 409, 508, 947, 1037
BssAI RCCGGY 6 cut(s) 194, 405, 409, 508, 947, 1037
BssECI CCNNGG 2 cut(s) 384, 732
BssHII GCGCGC 4 cut(s) 624, 643, 708, 1061
BssMI GATC 7 cut(s) 84, 398, 559, 765, 871, 973, 1224
BssNI GRCGYC 2 cut(s) 225, 351
BssSI CACGAG 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 384
Bst2BI CACGAG 1 cut(s) 80
Bst4CI ACNGT 2 cut(s) 36, 1267
Bst6I CTCTTC 4 cut(s) 168, 383, 545, 1230
BstACI GRCGYC 2 cut(s) 225, 351
BstAFI CTTAAG 1 cut(s) 584
BstBAI YACGTR 1 cut(s) 705
BstDEI CTNAG 4 cut(s) 74, 423, 519, 938
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 2 cut(s) 282, 712
BstFNI CGCG 8 cut(s) 368, 624, 626, 643, 645, 710, 1026, 1063
BstH2I RGCGCY 2 cut(s) 20, 228
BstKTI GATC 7 cut(s) 87, 401, 562, 768, 874, 976, 1227
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 7 cut(s) 84, 398, 559, 765, 871, 973, 1224
BstMCI CGRYCG 3 cut(s) 410, 509, 810
BstMWI GCNNNNNNNGC 9 cut(s) 11, 14, 288, 297, 357, 428, 707, 716, 728
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 1 cut(s) 1004
BstSLI GKGCMC 1 cut(s) 698
BstUI CGCG 8 cut(s) 368, 624, 626, 643, 645, 710, 1026, 1063
BstV1I GCAGC 6 cut(s) 20, 23, 26, 263, 401, 920
BstX2I RGATCY 2 cut(s) 84, 871
BstYI RGATCY 2 cut(s) 84, 871
BstZI CGGCCG 3 cut(s) 407, 506, 807
BsuRI GGCC 6 cut(s) 405, 409, 508, 731, 773, 809
BtgI CCRYGG 1 cut(s) 732
BtrI CACGTC 1 cut(s) 691
BtsCI GGATG 2 cut(s) 282, 712
BtsI GCAGTG 2 cut(s) 306, 417
BtsIMutI CAGTG 3 cut(s) 32, 306, 417
Cfr10I RCCGGY 6 cut(s) 194, 405, 409, 508, 947, 1037
Cfr13I GGNCC 4 cut(s) 403, 729, 1008, 1069
CseI GACGC 1 cut(s) 340
CviAII CATG 5 cut(s) 172, 613, 769, 793, 1066
DdeI CTNAG 4 cut(s) 74, 423, 519, 938
DinI GGCGCC 1 cut(s) 226
DpnI GATC 7 cut(s) 86, 400, 561, 767, 873, 975, 1226
DpnII GATC 7 cut(s) 84, 398, 559, 765, 871, 973, 1224
DrdI GACNNNNNNGTC 1 cut(s) 1288
DriI GACNNNNNGTC 1 cut(s) 616
DseDI GACNNNNNNGTC 1 cut(s) 1288
EaeI YGGCCR 4 cut(s) 407, 506, 771, 807
EagI CGGCCG 3 cut(s) 407, 506, 807
Eam1104I CTCTTC 4 cut(s) 168, 383, 545, 1230
Eam1105I GACNNNNNGTC 1 cut(s) 616
EarI CTCTTC 4 cut(s) 168, 383, 545, 1230
EciI GGCGGA 2 cut(s) 275, 798
EclXI CGGCCG 3 cut(s) 407, 506, 807
Eco130I CCWWGG 1 cut(s) 384
Eco47I GGWCC 2 cut(s) 1008, 1069
Eco47III AGCGCT 1 cut(s) 18
Eco52I CGGCCG 3 cut(s) 407, 506, 807
Eco57I CTGAAG 1 cut(s) 945
Eco72I CACGTG 1 cut(s) 705
Eco88I CYCGRG 2 cut(s) 98, 272
EcoT14I CCWWGG 1 cut(s) 384
EgeI GGCGCC 1 cut(s) 226
EheI GGCGCC 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 5 cut(s) 175, 616, 772, 796, 1069
FaiI YATR 9 cut(s) 44, 173, 614, 770, 794, 1020, 1046, 1067, 1106
FaqI GGGAC 1 cut(s) 604
FatI CATG 5 cut(s) 171, 612, 768, 792, 1065
FauI CCCGC 1 cut(s) 629
FbaI TGATCA 2 cut(s) 973, 1224
FblI GTMKAC 3 cut(s) 60, 476, 777
FokI GGATG 2 cut(s) 269, 699
FseI GGCCGGCC 1 cut(s) 409
FspBI CTAG 1 cut(s) 180
HaeII RGCGCY 2 cut(s) 20, 228
HaeIII GGCC 6 cut(s) 405, 409, 508, 731, 773, 809
HgaI GACGC 1 cut(s) 340
Hin1I GRCGYC 2 cut(s) 225, 351
Hin1II CATG 5 cut(s) 175, 616, 772, 796, 1069
HincII GTYRAC 3 cut(s) 61, 67, 778
HindII GTYRAC 3 cut(s) 61, 67, 778
HinfI GANTC 9 cut(s) 47, 207, 275, 302, 598, 902, 942, 1148, 1327
HphI GGTGA 3 cut(s) 184, 373, 1234
Hpy166II GTNNAC 7 cut(s) 61, 67, 268, 417, 477, 696, 778
Hpy188I TCNGA 8 cut(s) 206, 322, 804, 831, 893, 907, 914, 964
Hpy188III TCNNGA 4 cut(s) 98, 100, 425, 679
Hpy8I GTNNAC 7 cut(s) 61, 67, 268, 417, 477, 696, 778
HpyAV CCTTC 6 cut(s) 508, 517, 568, 679, 936, 947
HpyCH4III ACNGT 2 cut(s) 36, 1267
HpyCH4IV ACGT 6 cut(s) 63, 551, 690, 704, 825, 1143
HpyCH4V TGCA 3 cut(s) 282, 696, 1030
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 14, 288, 297, 357, 428, 707, 716, 728
HpyF3I CTNAG 4 cut(s) 74, 423, 519, 938
HpySE526I ACGT 6 cut(s) 63, 551, 690, 704, 825, 1143
Hsp92I GRCGYC 2 cut(s) 225, 351
Hsp92II CATG 5 cut(s) 175, 616, 772, 796, 1069
KasI GGCGCC 1 cut(s) 224
KroI GCCGGC 3 cut(s) 405, 508, 947
KroNI GCCGGC 3 cut(s) 407, 510, 949
Ksp22I TGATCA 2 cut(s) 973, 1224
Kzo9I GATC 7 cut(s) 84, 398, 559, 765, 871, 973, 1224
LguI GCTCTTC 2 cut(s) 383, 545
LmnI GCTCC 3 cut(s) 230, 350, 956
Lsp1109I GCAGC 6 cut(s) 20, 23, 26, 263, 401, 920
LweI GCATC 4 cut(s) 291, 640, 721, 1050
MaeI CTAG 1 cut(s) 180
MaeII ACGT 6 cut(s) 63, 551, 690, 704, 825, 1143
MaeIII GTNAC 2 cut(s) 119, 885
MalI GATC 7 cut(s) 86, 400, 561, 767, 873, 975, 1226
MboI GATC 7 cut(s) 84, 398, 559, 765, 871, 973, 1224
MflI RGATCY 2 cut(s) 84, 871
MhlI GDGCHC 2 cut(s) 698, 1187
MluCI AATT 3 cut(s) 786, 1015, 1189
Mly113I GGCGCC 1 cut(s) 225
MlyI GAGTC 6 cut(s) 41, 284, 296, 911, 1157, 1336
MmeI TCCRAC 4 cut(s) 827, 885, 1101, 1182
MreI CGCCGGCG 1 cut(s) 947
MroNI GCCGGC 3 cut(s) 405, 508, 947
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 4 cut(s) 212, 585, 741, 1115
MslI CAYNNNNRTG 1 cut(s) 1339
MspA1I CMGCKG 1 cut(s) 565
MspCI CTTAAG 1 cut(s) 584
MspR9I CCNGG 1 cut(s) 1006
Mva1269I GAATGC 1 cut(s) 360
MvnI CGCG 8 cut(s) 368, 624, 626, 643, 645, 710, 1026, 1063
MwoI GCNNNNNNNGC 9 cut(s) 11, 14, 288, 297, 357, 428, 707, 716, 728
NaeI GCCGGC 3 cut(s) 407, 510, 949
NarI GGCGCC 1 cut(s) 225
NciI CCSGG 1 cut(s) 1006
NdeII GATC 7 cut(s) 84, 398, 559, 765, 871, 973, 1224
NgoMIV GCCGGC 3 cut(s) 405, 508, 947
NlaIII CATG 5 cut(s) 175, 616, 772, 796, 1069
NlaIV GGNNCC 4 cut(s) 226, 232, 346, 730
NmuCI GTSAC 1 cut(s) 885
NspI RCATGY 1 cut(s) 616
PaeR7I CTCGAG 2 cut(s) 98, 272
PauI GCGCGC 4 cut(s) 624, 643, 708, 1061
PciI ACATGT 1 cut(s) 612
PciSI GCTCTTC 2 cut(s) 383, 545
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 1 cut(s) 360
PdiI GCCGGC 3 cut(s) 407, 510, 949
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 3 cut(s) 207, 598, 942
PfoI TCCNGGA 1 cut(s) 1004
PleI GAGTC 6 cut(s) 41, 283, 296, 910, 1156, 1335
PluTI GGCGCC 1 cut(s) 228
PmaCI CACGTG 1 cut(s) 705
PmlI CACGTG 1 cut(s) 705
PpsI GAGTC 6 cut(s) 41, 283, 296, 910, 1156, 1335
Ppu21I YACGTR 1 cut(s) 705
PscI ACATGT 1 cut(s) 612
PspCI CACGTG 1 cut(s) 705
PspN4I GGNNCC 4 cut(s) 226, 232, 346, 730
PspPI GGNCC 4 cut(s) 403, 729, 1008, 1069
PspXI VCTCGAGB 1 cut(s) 272
PsuI RGATCY 2 cut(s) 84, 871
PteI GCGCGC 4 cut(s) 624, 643, 708, 1061
PvuII CAGCTG 1 cut(s) 565
RigI GGCCGGCC 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 1339
SalI GTCGAC 2 cut(s) 59, 776
SapI GCTCTTC 2 cut(s) 383, 545
SaqAI TTAA 4 cut(s) 212, 585, 741, 1115
Sau3AI GATC 7 cut(s) 84, 398, 559, 765, 871, 973, 1224
Sau96I GGNCC 4 cut(s) 403, 729, 1008, 1069
SchI GAGTC 6 cut(s) 41, 284, 296, 911, 1157, 1336
ScrFI CCNGG 1 cut(s) 1006
SduI GDGCHC 2 cut(s) 698, 1187
SfaNI GCATC 4 cut(s) 291, 640, 721, 1050
SfoI GGCGCC 1 cut(s) 226
Sfr274I CTCGAG 2 cut(s) 98, 272
SgrAI CRCCGGYG 1 cut(s) 947
SgrDI CGTCGACG 2 cut(s) 59, 776
SinI GGWCC 2 cut(s) 1008, 1069
SlaI CTCGAG 2 cut(s) 98, 272
SmiMI CAYNNNNRTG 1 cut(s) 1339
SmlI CTYRAG 3 cut(s) 98, 272, 584
SmoI CTYRAG 3 cut(s) 98, 272, 584
Sse9I AATT 3 cut(s) 786, 1015, 1189
SspDI GGCGCC 1 cut(s) 224
SspI AATATT 1 cut(s) 489
SspMI CTAG 1 cut(s) 180
StyD4I CCNGG 1 cut(s) 1004
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 2 cut(s) 36, 1267
TaiI ACGT 6 cut(s) 66, 554, 693, 707, 828, 1146
TasI AATT 3 cut(s) 786, 1015, 1189
TauI GCSGC 8 cut(s) 8, 291, 314, 360, 371, 722, 812, 815
TfiI GAWTC 3 cut(s) 207, 598, 942
Tru1I TTAA 4 cut(s) 212, 585, 741, 1115
Tru9I TTAA 4 cut(s) 212, 585, 741, 1115
TscAI CASTG 3 cut(s) 39, 313, 424
TseFI GTSAC 1 cut(s) 885
TseI GCWGC 6 cut(s) 8, 11, 14, 251, 389, 933
Tsp45I GTSAC 1 cut(s) 885
TspDTI ATGAA 5 cut(s) 155, 188, 587, 698, 809
TspGWI ACGGA 3 cut(s) 46, 411, 840
TspRI CASTG 3 cut(s) 39, 313, 424
Vha464I CTTAAG 1 cut(s) 584
VneI GTGCAC 1 cut(s) 694
VpaK11BI GGWCC 2 cut(s) 1008, 1069
XapI RAATTY 1 cut(s) 1189
XceI RCATGY 1 cut(s) 616
XhoI CTCGAG 2 cut(s) 98, 272
XmiI GTMKAC 3 cut(s) 60, 476, 777
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.