Rroxscaffold_1G00059070

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015572
Physical Location & Seq
Reverse (-)
81057546 .. 81059962
2417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059070.1

Sequence Viewer

Length: 777 bp
ATGAAGGACACCCTTAAGTCGGTGTTGATTCCCTGCTACGACATGTCGTCCCGCGCGCCCTTTCTGTTTTCGCGCGCCGACGCTGTGGAGATGGACGGCTACGATTTCAAGATGAAGGACGTGTGCACCGCCACTTGCGCGCATCCGGCGGCGAAGGGGGCCGTGGAGGTTAAGTCAGTCGACGGGAGGACGAAGATCATGGCCGTCGACGGCGGAATTGCCATGAACAATCCGACGGCCGCGGCTATTACGCACGTGTTGAATAATAAGCAAGAGTTTCCGTTCTGTTCTGGTGTGGAGGATCTCTTGGTGGTGTCACTCGGAAATGGAGAGTCGGAGTTCGGAAACTTGTCGCCTTCTGGCTGCCTAAGAATCGCCGGCGAAGGAGCGTCAGACTTGGTTGATCAAGCTGTTTCAATGGCATTTGGGCAATCCCGGACCAGCAATTATGTTCGCGTGCAAGCCACCGGCATTATAGCCAAAAACACTGCGCGCATGGACCACTCCAACAACAAGAACAACACCAATACAGACATATTGGCTTTAACAGAGGAAATGTTGGCACAGAGAAACGTGGAGTCGATTTTATTCGAGGGGAAGAAAATGGTGGAGAGCACGAATTTGAAGAAGTTGGAGTTGTTCGCCGGAGAGGTGATCAAGGAAGAAGAGAGGAGAAAGACCAGCATTTTGCCGACGGTGGCTTTGAAGCAGACTTCGGGGTCGCCAGCCAGAACATCGTCTGCTACTACCCTATCAAGTCTGTCATCTTCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

258

Amino Acids

27.61

Weight (kDa)

5.99

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015572)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11650
malus_domestica MD05G1261500.v1.1
prunus_persica Prupe.4G104000_v2.0.a1
pyrus_communis pycom05g24510 pycom10g20060
rosa_chinensis RchiOBHm_Chr5g0018711
rosa_laevigata RLG00000032389
rosa_multiflora Rmu_co8494637.1_g000001
rosa_roxburghii Rroxscaffold_1G00059070 Rroxscaffold_1G00073800
rosa_rugosa Rorug05G0046100
rosa_samantha Rh5AG137100 Rh5BG135900 Rh5CG147900 Rh5DG137000
rosa_wichuraiana Rw5G012150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 718
AccI GTMKAC 2 cut(s) 180, 207
AccII CGCG 8 cut(s) 54, 56, 73, 75, 140, 242, 456, 493
AciI CCGC 6 cut(s) 52, 129, 149, 213, 240, 242
AclWI GGATC 1 cut(s) 309
AcoI YGGCCR 2 cut(s) 201, 237
AcsI RAATTY 1 cut(s) 619
AcvI CACGTG 1 cut(s) 256
AfiI CCNNNNNNNGG 1 cut(s) 19
AflII CTTAAG 1 cut(s) 14
AflIII ACRYGT 3 cut(s) 42, 120, 255
AgsI TTSAA 5 cut(s) 109, 262, 417, 625, 706
AhdI GACNNNNNGTC 1 cut(s) 46
AjiI CACGTC 1 cut(s) 121
AluBI AGCT 1 cut(s) 410
AluI AGCT 1 cut(s) 410
Alw21I GWGCWC 2 cut(s) 128, 617
Alw44I GTGCAC 1 cut(s) 124
AlwI GGATC 1 cut(s) 309
AoxI GGCC 3 cut(s) 159, 201, 237
ApaLI GTGCAC 1 cut(s) 124
ApeKI GCWGC 1 cut(s) 363
ApoI RAATTY 1 cut(s) 619
ArsI GACNNNNNNTTYG 4 cut(s) 670, 685, 702, 717
AspLEI GCGC 8 cut(s) 56, 58, 75, 77, 140, 142, 493, 495
AspS9I GGNCC 3 cut(s) 159, 438, 499
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 1 cut(s) 664
AvaII GGWCC 2 cut(s) 438, 499
BaeGI GKGCMC 1 cut(s) 128
BbrPI CACGTG 1 cut(s) 256
Bbv12I GWGCWC 2 cut(s) 128, 617
BbvI GCAGC 1 cut(s) 350
BccI CCATC 1 cut(s) 85
BceAI ACGGC 5 cut(s) 112, 146, 188, 226, 252
BclI TGATCA 2 cut(s) 403, 654
BcnI CCSGG 1 cut(s) 436
BfrI CTTAAG 1 cut(s) 14
BisI GCNGC 4 cut(s) 150, 240, 243, 364
BlsI GCNGC 4 cut(s) 151, 241, 244, 365
Bme1390I CCNGG 1 cut(s) 436
Bme18I GGWCC 2 cut(s) 438, 499
BmeRI GACNNNNNGTC 1 cut(s) 46
BmgBI CACGTC 1 cut(s) 121
BmgT120I GGNCC 3 cut(s) 159, 438, 499
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 1 cut(s) 436
BmsI GCATC 1 cut(s) 151
BpuMI CCSGG 1 cut(s) 436
BsaAI YACGTR 1 cut(s) 256
BsaJI CCNNGG 2 cut(s) 162, 240
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 1 cut(s) 19
Bse118I RCCGGY 2 cut(s) 377, 467
BseDI CCNNGG 2 cut(s) 162, 240
BseGI GGATG 1 cut(s) 142
BseLI CCNNNNNNNGG 1 cut(s) 19
BsePI GCGCGC 4 cut(s) 54, 73, 138, 491
BseRI GAGGAG 1 cut(s) 685
BseSI GKGCMC 1 cut(s) 128
BseX3I CGGCCG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 350
Bsh1236I CGCG 8 cut(s) 54, 56, 73, 75, 140, 242, 456, 493
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 3 cut(s) 161, 203, 239
BsiEI CGRYCG 1 cut(s) 240
BsiHKAI GWGCWC 2 cut(s) 128, 617
BsiSI CCGG 5 cut(s) 146, 378, 436, 468, 645
BslFI GGGAC 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 34
BsnI GGCC 3 cut(s) 161, 203, 239
Bsp1286I GDGCHC 2 cut(s) 128, 617
Bsp143I GATC 4 cut(s) 195, 301, 403, 654
BspACI CCGC 6 cut(s) 52, 129, 149, 213, 240, 242
BspANI GGCC 3 cut(s) 161, 203, 239
BspFNI CGCG 8 cut(s) 54, 56, 73, 75, 140, 242, 456, 493
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 1 cut(s) 309
BspTI CTTAAG 1 cut(s) 14
BsrFI RCCGGY 2 cut(s) 377, 467
BssAI RCCGGY 2 cut(s) 377, 467
BssECI CCNNGG 2 cut(s) 162, 240
BssHII GCGCGC 4 cut(s) 54, 73, 138, 491
BssMI GATC 4 cut(s) 195, 301, 403, 654
Bst4CI ACNGT 1 cut(s) 697
Bst6I CTCTTC 1 cut(s) 660
BstAFI CTTAAG 1 cut(s) 14
BstBAI YACGTR 1 cut(s) 256
BstC8I GCNNGC 8 cut(s) 56, 75, 140, 379, 458, 462, 493, 726
BstDEI CTNAG 1 cut(s) 368
BstDSI CCRYGG 2 cut(s) 162, 240
BstF5I GGATG 1 cut(s) 142
BstFNI CGCG 8 cut(s) 54, 56, 73, 75, 140, 242, 456, 493
BstHHI GCGC 8 cut(s) 56, 58, 75, 77, 140, 142, 493, 495
BstKTI GATC 4 cut(s) 198, 304, 406, 657
BstMBI GATC 4 cut(s) 195, 301, 403, 654
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 3 cut(s) 137, 146, 158
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 434
BstSLI GKGCMC 1 cut(s) 128
BstUI CGCG 8 cut(s) 54, 56, 73, 75, 140, 242, 456, 493
BstV1I GCAGC 1 cut(s) 350
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BstZI CGGCCG 1 cut(s) 237
BsuRI GGCC 3 cut(s) 161, 203, 239
BtgI CCRYGG 2 cut(s) 162, 240
BtrI CACGTC 1 cut(s) 121
BtsCI GGATG 1 cut(s) 142
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 1 cut(s) 486
Cac8I GCNNGC 8 cut(s) 56, 75, 140, 379, 458, 462, 493, 726
CfoI GCGC 8 cut(s) 56, 58, 75, 77, 140, 142, 493, 495
Cfr10I RCCGGY 2 cut(s) 377, 467
Cfr13I GGNCC 3 cut(s) 159, 438, 499
Cfr42I CCGCGG 1 cut(s) 243
CseI GACGC 2 cut(s) 89, 378
CviAII CATG 4 cut(s) 43, 199, 223, 496
DdeI CTNAG 1 cut(s) 368
DpnI GATC 4 cut(s) 197, 303, 405, 656
DpnII GATC 4 cut(s) 195, 301, 403, 654
DrdI GACNNNNNNGTC 1 cut(s) 718
DriI GACNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 718
EaeI YGGCCR 2 cut(s) 201, 237
EagI CGGCCG 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 660
Eam1105I GACNNNNNGTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 660
EciI GGCGGA 1 cut(s) 228
EclXI CGGCCG 1 cut(s) 237
Eco47I GGWCC 2 cut(s) 438, 499
Eco52I CGGCCG 1 cut(s) 237
Eco72I CACGTG 1 cut(s) 256
FaeI CATG 4 cut(s) 46, 202, 226, 499
FaiI YATR 7 cut(s) 44, 200, 224, 450, 476, 497, 536
FaqI GGGAC 1 cut(s) 34
FatI CATG 4 cut(s) 42, 198, 222, 495
FauI CCCGC 1 cut(s) 59
FbaI TGATCA 2 cut(s) 403, 654
FblI GTMKAC 2 cut(s) 180, 207
Fnu4HI GCNGC 4 cut(s) 150, 240, 243, 364
FokI GGATG 1 cut(s) 129
Fsp4HI GCNGC 4 cut(s) 150, 240, 243, 364
GlaI GCGC 8 cut(s) 55, 57, 74, 76, 139, 141, 492, 494
GluI GCNGC 4 cut(s) 150, 240, 243, 364
HaeIII GGCC 3 cut(s) 161, 203, 239
HapII CCGG 5 cut(s) 146, 378, 436, 468, 645
HgaI GACGC 2 cut(s) 89, 378
HhaI GCGC 8 cut(s) 56, 58, 75, 77, 140, 142, 493, 495
Hin1II CATG 4 cut(s) 46, 202, 226, 499
Hin6I GCGC 8 cut(s) 54, 56, 73, 75, 138, 140, 491, 493
HinP1I GCGC 8 cut(s) 54, 56, 73, 75, 138, 140, 491, 493
HincII GTYRAC 2 cut(s) 181, 208
HindII GTYRAC 2 cut(s) 181, 208
HinfI GANTC 4 cut(s) 28, 332, 372, 578
HpaII CCGG 5 cut(s) 146, 378, 436, 468, 645
HphI GGTGA 1 cut(s) 664
Hpy166II GTNNAC 3 cut(s) 126, 181, 208
Hpy188I TCNGA 5 cut(s) 234, 323, 337, 344, 394
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 3 cut(s) 126, 181, 208
Hpy99I CGWCG 6 cut(s) 83, 185, 209, 212, 238, 697
HpyAV CCTTC 4 cut(s) 109, 148, 366, 377
HpyCH4III ACNGT 1 cut(s) 697
HpyCH4IV ACGT 3 cut(s) 120, 255, 573
HpyCH4V TGCA 2 cut(s) 126, 460
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 146, 158
HpyF3I CTNAG 1 cut(s) 368
HpySE526I ACGT 3 cut(s) 120, 255, 573
Hsp92II CATG 4 cut(s) 46, 202, 226, 499
HspAI GCGC 8 cut(s) 54, 56, 73, 75, 138, 140, 491, 493
KroI GCCGGC 1 cut(s) 377
KroNI GCCGGC 1 cut(s) 379
Ksp22I TGATCA 2 cut(s) 403, 654
KspI CCGCGG 1 cut(s) 243
Kzo9I GATC 4 cut(s) 195, 301, 403, 654
LmnI GCTCC 1 cut(s) 386
Lsp1109I GCAGC 1 cut(s) 350
LweI GCATC 1 cut(s) 151
MaeII ACGT 3 cut(s) 120, 255, 573
MaeIII GTNAC 1 cut(s) 315
MalI GATC 4 cut(s) 197, 303, 405, 656
MboI GATC 4 cut(s) 195, 301, 403, 654
MboII GAAGA 6 cut(s) 205, 610, 637, 674, 677, 759
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 2 cut(s) 128, 617
MluCI AATT 3 cut(s) 216, 445, 619
MlyI GAGTC 2 cut(s) 341, 587
MmeI TCCRAC 4 cut(s) 257, 315, 531, 612
MnlI CCTC 7 cut(s) 160, 180, 292, 544, 586, 643, 663
MreI CGCCGGCG 1 cut(s) 377
MroNI GCCGGC 1 cut(s) 377
MseI TTAA 3 cut(s) 15, 171, 545
MslI CAYNNNNRTG 1 cut(s) 769
MspA1I CMGCKG 1 cut(s) 242
MspCI CTTAAG 1 cut(s) 14
MspI CCGG 5 cut(s) 146, 378, 436, 468, 645
MspR9I CCNGG 1 cut(s) 436
MvnI CGCG 8 cut(s) 54, 56, 73, 75, 140, 242, 456, 493
MwoI GCNNNNNNNGC 3 cut(s) 137, 146, 158
NaeI GCCGGC 1 cut(s) 379
NciI CCSGG 1 cut(s) 436
NdeII GATC 4 cut(s) 195, 301, 403, 654
NgoMIV GCCGGC 1 cut(s) 377
NlaIII CATG 4 cut(s) 46, 202, 226, 499
NlaIV GGNNCC 1 cut(s) 160
NmuCI GTSAC 1 cut(s) 315
NspI RCATGY 1 cut(s) 46
PauI GCGCGC 4 cut(s) 54, 73, 138, 491
PciI ACATGT 1 cut(s) 42
PdiI GCCGGC 1 cut(s) 379
PfeI GAWTC 2 cut(s) 28, 372
PfoI TCCNGGA 1 cut(s) 434
PkrI GCNGC 4 cut(s) 151, 241, 244, 365
PleI GAGTC 2 cut(s) 340, 586
PmaCI CACGTG 1 cut(s) 256
PmlI CACGTG 1 cut(s) 256
PpsI GAGTC 2 cut(s) 340, 586
Ppu21I YACGTR 1 cut(s) 256
PscI ACATGT 1 cut(s) 42
PspCI CACGTG 1 cut(s) 256
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 3 cut(s) 159, 438, 499
PsuI RGATCY 1 cut(s) 301
PteI GCGCGC 4 cut(s) 54, 73, 138, 491
RseI CAYNNNNRTG 1 cut(s) 769
SacII CCGCGG 1 cut(s) 243
SalI GTCGAC 2 cut(s) 179, 206
SaqAI TTAA 3 cut(s) 15, 171, 545
SatI GCNGC 4 cut(s) 150, 240, 243, 364
Sau3AI GATC 4 cut(s) 195, 301, 403, 654
Sau96I GGNCC 3 cut(s) 159, 438, 499
SchI GAGTC 2 cut(s) 341, 587
ScrFI CCNGG 1 cut(s) 436
SduI GDGCHC 2 cut(s) 128, 617
SetI ASST 6 cut(s) 123, 171, 258, 412, 576, 654
SfaNI GCATC 1 cut(s) 151
Sfr303I CCGCGG 1 cut(s) 243
SgrAI CRCCGGYG 1 cut(s) 377
SgrBI CCGCGG 1 cut(s) 243
SgrDI CGTCGACG 1 cut(s) 206
SinI GGWCC 2 cut(s) 438, 499
SmiMI CAYNNNNRTG 1 cut(s) 769
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
Sse9I AATT 3 cut(s) 216, 445, 619
SsiI CCGC 6 cut(s) 52, 129, 149, 213, 240, 242
StyD4I CCNGG 1 cut(s) 434
TaaI ACNGT 1 cut(s) 697
TaiI ACGT 3 cut(s) 123, 258, 576
TaqI TCGA 4 cut(s) 180, 207, 581, 591
TasI AATT 3 cut(s) 216, 445, 619
TauI GCSGC 3 cut(s) 152, 242, 245
TfiI GAWTC 2 cut(s) 28, 372
Tru1I TTAA 3 cut(s) 15, 171, 545
Tru9I TTAA 3 cut(s) 15, 171, 545
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 1 cut(s) 315
TseI GCWGC 1 cut(s) 363
Tsp45I GTSAC 1 cut(s) 315
TspDTI ATGAA 3 cut(s) 17, 128, 239
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 1 cut(s) 493
Vha464I CTTAAG 1 cut(s) 14
VneI GTGCAC 1 cut(s) 124
VpaK11BI GGWCC 2 cut(s) 438, 499
XapI RAATTY 1 cut(s) 619
XceI RCATGY 1 cut(s) 46
XmiI GTMKAC 2 cut(s) 180, 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.