MD05G1261500.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015572
Physical Location & Seq
Reverse (-)
39680748 .. 39683181
2434 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1261500.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGGCGGCTCCCATGCTCAACGTTGACACAAACTTGCTCGACGTCGACAAGCTCTCCTACGAAATCTTCTCCGTTCTCGAGAACAACTTCCTCTTCGGAAACCCACCAAAACCAAAAGCCTTCGACCCCAACTCCGCCGATCTCTCCACTCCTCTCCACCACATGCATTCTAGCAAACAGTTTACCGGAAAAGTCAGAATTCTCACCATCGACGCCGCCGGAGCCACTGATGGCATCCTTGCCGCCAATGCTCTCTCCCACCTCGAGTCCTCTCTCCGCCGGAAATCCGGCAACCCCAACGCCGCCATCTCCGACTTCTTCGACGTCGTTTCCGGGTCAGGTGCCGGCGGCATCCTCGCCGCCCTCCTCTTCACTCGAGGTTTAAAGGAAGGGTCGACCCGACCCATGTTCACCGCCAAAGAAGCCCTCGACTTCCTTATCGAGAACCGCCGCAAGATCTTCAAACCATCTCCGGATGGGGTTCTCCGGCGGGTTTTCCGGCCGTCGATGGCTGAGAAGGAGAGGGAGAAACTCTTTCGGAAGACGTTCGGGGAGCAGCTGACTCTCAGGGACACGCTTAAGTCGGTGCTGATCCCCTGCTACGACATGTCGTCCTGCGCGCCGTTTTTGTTTTCGCGGGCGGACGCGGTGGAGATGGACGGCTACGATTTTAAGATGATGGACGTGTGCGCTGCCACGTCGGCTCAACCGGCCGTTGAGGTGAGGTCGGTGGACGGGAGAACAAAGCTCATGGCCGTTGATGGTAGTATCGCTATGAACAATCCGACGGCTGCAGCTATTACGCACGTGCTAAATAATAAGCAGGAATTTCCGTTCTGTAACGACGTTGAGGATCTCTTGGTCGTGTCTCTCGGAAATGGAGAGTCCGAGTTTGGAAATTTGTCGCCTTCTGGTTGCCTCAGAATCGCCGGTGAAGGAGCTTCAGACTTGGTTGATCAAGCCGTTTCAATGGCTTTTGGACAATCCCGAGCAACCAATTATGTTCGCGTGCAAGCCAACGGCATCATATCTAAAATGCATGGAGGCCCCCAAAACTCCAACGGCAAAAATAAGAACAAGATAGACATGTTGGCCTTGACAGAGGAAATGTTGTCACAGAAAAATGTGGAGTCCATATTATTCCAAGGTAAAAAAATGGTGAAATGCACAAATGTGGAGAAGCTAGAAATGTTTTCCGGGGAGCTGATTAAGGAGCAAGAGAGGAGAAAAACTTGTATTCTTCCAACCGTGGCGTTGAAGCAGACTTCTTCGTTATCACCCAGCAGAACATCGTCTGCCACAACCTTATCTATGTCGTCGTCATGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

444

Amino Acids

48.12

Weight (kDa)

8.04

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 72 - 267 1e-12 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015572)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11650
malus_domestica MD05G1261500.v1.1
prunus_persica Prupe.4G104000_v2.0.a1
pyrus_communis pycom05g24510 pycom10g20060
rosa_chinensis RchiOBHm_Chr5g0018711
rosa_laevigata RLG00000032389
rosa_multiflora Rmu_co8494637.1_g000001
rosa_roxburghii Rroxscaffold_1G00059070 Rroxscaffold_1G00073800
rosa_rugosa Rorug05G0046100
rosa_samantha Rh5AG137100 Rh5BG135900 Rh5CG147900 Rh5DG137000
rosa_wichuraiana Rw5G012150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 45, 327
AccB1I GGYRCC 1 cut(s) 341
AccI GTMKAC 2 cut(s) 45, 395
AccII CGCG 4 cut(s) 620, 637, 647, 1008
AccIII TCCGGA 1 cut(s) 472
AclI AACGTT 1 cut(s) 21
AclWI GGATC 2 cut(s) 586, 861
AcoI YGGCCR 3 cut(s) 500, 711, 753
AcsI RAATTY 3 cut(s) 198, 827, 898
AcuI CTGAAG 1 cut(s) 927
AcvI CACGTG 1 cut(s) 808
AcyI GRCGYC 3 cut(s) 42, 213, 324
AflII CTTAAG 1 cut(s) 578
AflIII ACRYGT 3 cut(s) 606, 684, 1086
AgsI TTSAA 3 cut(s) 463, 969, 1258
AhdI GACNNNNNGTC 1 cut(s) 610
AjiI CACGTC 2 cut(s) 685, 699
AleI CACNNNNGTG 1 cut(s) 1172
AloI GAACNNNNNNTCC 2 cut(s) 818, 850
AluBI AGCT 7 cut(s) 52, 559, 748, 797, 941, 1183, 1204
AluI AGCT 7 cut(s) 52, 559, 748, 797, 941, 1183, 1204
Alw26I GTCTC 1 cut(s) 873
AlwI GGATC 2 cut(s) 586, 861
Ama87I CYCGRG 4 cut(s) 77, 263, 375, 987
Aor13HI TCCGGA 1 cut(s) 472
AoxI GGCC 5 cut(s) 500, 711, 753, 1045, 1092
ApeKI GCWGC 4 cut(s) 556, 692, 791, 794
ApoI RAATTY 3 cut(s) 198, 827, 898
Asp700I GAANNNNTTC 2 cut(s) 86, 545
AspLEI GCGC 3 cut(s) 620, 622, 692
AspS9I GGNCC 1 cut(s) 1046
AsuC2I CCSGG 2 cut(s) 334, 1198
AsuHPI GGTGA 6 cut(s) 196, 403, 733, 944, 1171, 1269
AvaI CYCGRG 4 cut(s) 77, 263, 375, 987
BanI GGYRCC 1 cut(s) 341
BbrPI CACGTG 1 cut(s) 808
BbsI GAAGAC 1 cut(s) 548
BbvI GCAGC 4 cut(s) 568, 679, 778, 806
BccI CCATC 9 cut(s) 215, 224, 314, 470, 475, 502, 649, 673, 755
BceAI ACGGC 9 cut(s) 487, 607, 676, 698, 740, 804, 947, 1036, 1078
BclI TGATCA 1 cut(s) 955
BcnI CCSGG 2 cut(s) 334, 1198
BcoDI GTCTC 1 cut(s) 873
BfaI CTAG 2 cut(s) 171, 1184
BfmI CTRYAG 1 cut(s) 792
BfrI CTTAAG 1 cut(s) 578
BglI GCCNNNNNGGC 1 cut(s) 701
BglII AGATCT 1 cut(s) 456
Bme1390I CCNGG 2 cut(s) 334, 1198
BmeRI GACNNNNNGTC 1 cut(s) 610
BmeT110I CYCGRG 4 cut(s) 77, 263, 375, 987
BmgBI CACGTC 2 cut(s) 685, 699
BmgT120I GGNCC 1 cut(s) 1046
BmiI GGNNCC 4 cut(s) 9, 223, 343, 1048
BmrFI CCNGG 2 cut(s) 334, 1198
BmsI GCATC 3 cut(s) 243, 360, 1032
BpiI GAAGAC 1 cut(s) 548
BpuMI CCSGG 2 cut(s) 334, 1198
BsaAI YACGTR 1 cut(s) 808
BsaHI GRCGYC 3 cut(s) 42, 213, 324
BsaJI CCNNGG 3 cut(s) 1144, 1197, 1248
BsaWI WCCGGW 2 cut(s) 185, 472
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 116, 146
Bse118I RCCGGY 3 cut(s) 344, 709, 929
BseAI TCCGGA 1 cut(s) 472
BseDI CCNNGG 3 cut(s) 1144, 1197, 1248
BseGI GGATG 3 cut(s) 234, 351, 481
BseMII CTCAG 3 cut(s) 504, 580, 934
BsePI GCGCGC 1 cut(s) 618
BseRI GAGGAG 3 cut(s) 141, 356, 1237
BseX3I CGGCCG 2 cut(s) 500, 711
BseXI GCAGC 4 cut(s) 568, 679, 778, 806
BseYI CCCAGC 1 cut(s) 1280
Bsh1236I CGCG 4 cut(s) 620, 637, 647, 1008
Bsh1285I CGRYCG 2 cut(s) 503, 714
BshFI GGCC 5 cut(s) 502, 713, 755, 1047, 1094
BshNI GGYRCC 1 cut(s) 341
BsiEI CGRYCG 2 cut(s) 503, 714
BsiHKCI CYCGRG 4 cut(s) 77, 263, 375, 987
BslFI GGGAC 1 cut(s) 584
BsmAI GTCTC 1 cut(s) 873
BsmFI GGGAC 1 cut(s) 584
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 5 cut(s) 502, 713, 755, 1047, 1094
BsoBI CYCGRG 4 cut(s) 77, 263, 375, 987
Bsp13I TCCGGA 1 cut(s) 472
Bsp143I GATC 5 cut(s) 139, 456, 591, 853, 955
BspANI GGCC 5 cut(s) 502, 713, 755, 1047, 1094
BspCNI CTCAG 3 cut(s) 505, 579, 933
BspEI TCCGGA 1 cut(s) 472
BspFNI CGCG 4 cut(s) 620, 637, 647, 1008
BspLI GGNNCC 4 cut(s) 9, 223, 343, 1048
BspMAI CTGCAG 1 cut(s) 796
BspPI GGATC 2 cut(s) 586, 861
BspT107I GGYRCC 1 cut(s) 341
BspTI CTTAAG 1 cut(s) 578
BsrFI RCCGGY 3 cut(s) 344, 709, 929
BssAI RCCGGY 3 cut(s) 344, 709, 929
BssECI CCNNGG 3 cut(s) 1144, 1197, 1248
BssHII GCGCGC 1 cut(s) 618
BssMI GATC 5 cut(s) 139, 456, 591, 853, 955
BssNI GRCGYC 3 cut(s) 42, 213, 324
BssT1I CCWWGG 1 cut(s) 1144
Bst4CI ACNGT 2 cut(s) 180, 1249
Bst6I CTCTTC 2 cut(s) 98, 374
BstACI GRCGYC 3 cut(s) 42, 213, 324
BstAFI CTTAAG 1 cut(s) 578
BstBAI YACGTR 1 cut(s) 808
BstC8I GCNNGC 5 cut(s) 346, 620, 639, 1010, 1014
BstDEI CTNAG 3 cut(s) 513, 566, 920
BstDSI CCRYGG 1 cut(s) 1248
BstF5I GGATG 3 cut(s) 234, 351, 481
BstFNI CGCG 4 cut(s) 620, 637, 647, 1008
BstHHI GCGC 3 cut(s) 620, 622, 692
BstKTI GATC 5 cut(s) 142, 459, 594, 856, 958
BstMAI GTCTC 1 cut(s) 873
BstMBI GATC 5 cut(s) 139, 456, 591, 853, 955
BstMCI CGRYCG 2 cut(s) 503, 714
BstMWI GCNNNNNNNGC 5 cut(s) 221, 248, 422, 701, 710
BstNSI RCATGY 3 cut(s) 166, 610, 1090
BstSCI CCNGG 2 cut(s) 332, 1196
BstSFI CTRYAG 1 cut(s) 792
BstUI CGCG 4 cut(s) 620, 637, 647, 1008
BstV1I GCAGC 4 cut(s) 568, 679, 778, 806
BstV2I GAAGAC 1 cut(s) 548
BstX2I RGATCY 2 cut(s) 456, 853
BstYI RGATCY 2 cut(s) 456, 853
BstZI CGGCCG 2 cut(s) 500, 711
BsuRI GGCC 5 cut(s) 502, 713, 755, 1047, 1094
BtgI CCRYGG 1 cut(s) 1248
BtrI CACGTC 2 cut(s) 685, 699
BtsCI GGATG 3 cut(s) 234, 351, 481
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 5 cut(s) 346, 620, 639, 1010, 1014
CfoI GCGC 3 cut(s) 620, 622, 692
Cfr10I RCCGGY 3 cut(s) 344, 709, 929
Cfr13I GGNCC 1 cut(s) 1046
CseI GACGC 2 cut(s) 221, 653
CviAII CATG 8 cut(s) 13, 163, 406, 607, 751, 1040, 1087, 1323
DdeI CTNAG 3 cut(s) 513, 566, 920
DpnI GATC 5 cut(s) 141, 458, 593, 855, 957
DpnII GATC 5 cut(s) 139, 456, 591, 853, 955
DraI TTTAAA 1 cut(s) 384
DriI GACNNNNNGTC 1 cut(s) 610
EaeI YGGCCR 3 cut(s) 500, 711, 753
EagI CGGCCG 2 cut(s) 500, 711
Eam1104I CTCTTC 2 cut(s) 98, 374
Eam1105I GACNNNNNGTC 1 cut(s) 610
EarI CTCTTC 2 cut(s) 98, 374
EciI GGCGGA 3 cut(s) 124, 266, 656
EclXI CGGCCG 2 cut(s) 500, 711
Eco130I CCWWGG 1 cut(s) 1144
Eco52I CGGCCG 2 cut(s) 500, 711
Eco57I CTGAAG 1 cut(s) 927
Eco72I CACGTG 1 cut(s) 808
Eco88I CYCGRG 4 cut(s) 77, 263, 375, 987
EcoO109I RGGNCCY 1 cut(s) 1046
EcoRI GAATTC 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 1144
EcoT22I ATGCAT 2 cut(s) 168, 1041
ErhI CCWWGG 1 cut(s) 1144
FaeI CATG 8 cut(s) 16, 166, 409, 610, 754, 1043, 1090, 1326
FaqI GGGAC 1 cut(s) 584
FatI CATG 8 cut(s) 12, 162, 405, 606, 750, 1039, 1086, 1322
FauI CCCGC 2 cut(s) 483, 630
FbaI TGATCA 1 cut(s) 955
FblI GTMKAC 2 cut(s) 45, 395
FokI GGATG 3 cut(s) 221, 338, 488
FspBI CTAG 2 cut(s) 171, 1184
GlaI GCGC 3 cut(s) 619, 621, 691
GsaI CCCAGC 1 cut(s) 1284
HaeIII GGCC 5 cut(s) 502, 713, 755, 1047, 1094
HgaI GACGC 2 cut(s) 221, 653
HhaI GCGC 3 cut(s) 620, 622, 692
Hin1I GRCGYC 3 cut(s) 42, 213, 324
Hin1II CATG 8 cut(s) 16, 166, 409, 610, 754, 1043, 1090, 1326
Hin6I GCGC 3 cut(s) 618, 620, 690
HinP1I GCGC 3 cut(s) 618, 620, 690
HincII GTYRAC 3 cut(s) 25, 46, 396
HindII GTYRAC 3 cut(s) 25, 46, 396
HinfI GANTC 5 cut(s) 266, 562, 884, 924, 1130
HphI GGTGA 6 cut(s) 196, 403, 733, 944, 1171, 1269
Hpy166II GTNNAC 6 cut(s) 25, 46, 183, 396, 411, 733
Hpy188I TCNGA 9 cut(s) 98, 197, 313, 540, 786, 875, 889, 923, 946
Hpy188III TCNNGA 5 cut(s) 77, 79, 442, 473, 987
Hpy8I GTNNAC 6 cut(s) 25, 46, 183, 396, 411, 733
HpyAV CCTTC 5 cut(s) 130, 383, 511, 918, 929
HpyCH4III ACNGT 2 cut(s) 180, 1249
HpyCH4IV ACGT 8 cut(s) 21, 42, 324, 545, 684, 698, 807, 846
HpyCH4V TGCA 5 cut(s) 166, 794, 1012, 1039, 1167
HpyF10VI GCNNNNNNNGC 5 cut(s) 221, 248, 422, 701, 710
HpyF3I CTNAG 3 cut(s) 513, 566, 920
HpySE526I ACGT 8 cut(s) 21, 42, 324, 545, 684, 698, 807, 846
Hsp92I GRCGYC 3 cut(s) 42, 213, 324
Hsp92II CATG 8 cut(s) 16, 166, 409, 610, 754, 1043, 1090, 1326
HspAI GCGC 3 cut(s) 618, 620, 690
Kpn2I TCCGGA 1 cut(s) 472
KroI GCCGGC 1 cut(s) 344
KroNI GCCGGC 1 cut(s) 346
Ksp22I TGATCA 1 cut(s) 955
Kzo9I GATC 5 cut(s) 139, 456, 591, 853, 955
LmnI GCTCC 6 cut(s) 13, 221, 553, 938, 1201, 1213
Lsp1109I GCAGC 4 cut(s) 568, 679, 778, 806
LweI GCATC 3 cut(s) 243, 360, 1032
MaeI CTAG 2 cut(s) 171, 1184
MaeII ACGT 8 cut(s) 21, 42, 324, 545, 684, 698, 807, 846
MaeIII GTNAC 2 cut(s) 839, 1113
MalI GATC 5 cut(s) 141, 458, 593, 855, 957
MboI GATC 5 cut(s) 139, 456, 591, 853, 955
MboII GAAGA 8 cut(s) 58, 85, 310, 361, 451, 553, 1232, 1260
MflI RGATCY 2 cut(s) 456, 853
MluCI AATT 5 cut(s) 198, 827, 898, 997, 1327
MlyI GAGTC 4 cut(s) 275, 556, 893, 1139
MmeI TCCRAC 4 cut(s) 336, 809, 1083, 1268
Mph1103I ATGCAT 2 cut(s) 168, 1041
MroI TCCGGA 1 cut(s) 472
MroNI GCCGGC 1 cut(s) 344
MroXI GAANNNNTTC 2 cut(s) 86, 545
MseI TTAA 4 cut(s) 383, 579, 672, 1209
MslI CAYNNNNRTG 1 cut(s) 1172
MspA1I CMGCKG 1 cut(s) 559
MspCI CTTAAG 1 cut(s) 578
MspR9I CCNGG 2 cut(s) 334, 1198
Mva1269I GAATGC 1 cut(s) 166
MvnI CGCG 4 cut(s) 620, 637, 647, 1008
MwoI GCNNNNNNNGC 5 cut(s) 221, 248, 422, 701, 710
NaeI GCCGGC 1 cut(s) 346
NciI CCSGG 2 cut(s) 334, 1198
NdeII GATC 5 cut(s) 139, 456, 591, 853, 955
NgoMIV GCCGGC 1 cut(s) 344
NlaIII CATG 8 cut(s) 16, 166, 409, 610, 754, 1043, 1090, 1326
NlaIV GGNNCC 4 cut(s) 9, 223, 343, 1048
NmuCI GTSAC 1 cut(s) 1113
NsiI ATGCAT 2 cut(s) 168, 1041
NspI RCATGY 3 cut(s) 166, 610, 1090
OliI CACNNNNGTG 1 cut(s) 1172
PaeR7I CTCGAG 3 cut(s) 77, 263, 375
PauI GCGCGC 1 cut(s) 618
PciI ACATGT 2 cut(s) 606, 1086
PctI GAATGC 1 cut(s) 166
PdiI GCCGGC 1 cut(s) 346
PdmI GAANNNNTTC 2 cut(s) 86, 545
PfeI GAWTC 1 cut(s) 924
PleI GAGTC 4 cut(s) 274, 556, 892, 1138
PmaCI CACGTG 1 cut(s) 808
PmlI CACGTG 1 cut(s) 808
PpsI GAGTC 4 cut(s) 274, 556, 892, 1138
Ppu21I YACGTR 1 cut(s) 808
PscI ACATGT 2 cut(s) 606, 1086
Psp1406I AACGTT 1 cut(s) 21
PspCI CACGTG 1 cut(s) 808
PspFI CCCAGC 1 cut(s) 1280
PspN4I GGNNCC 4 cut(s) 9, 223, 343, 1048
PspPI GGNCC 1 cut(s) 1046
PspXI VCTCGAGB 2 cut(s) 263, 375
PstI CTGCAG 1 cut(s) 796
PsuI RGATCY 2 cut(s) 456, 853
PteI GCGCGC 1 cut(s) 618
PvuII CAGCTG 1 cut(s) 559
RseI CAYNNNNRTG 1 cut(s) 1172
SalI GTCGAC 2 cut(s) 44, 394
SaqAI TTAA 4 cut(s) 383, 579, 672, 1209
Sau3AI GATC 5 cut(s) 139, 456, 591, 853, 955
Sau96I GGNCC 1 cut(s) 1046
SchI GAGTC 4 cut(s) 275, 556, 893, 1139
ScrFI CCNGG 2 cut(s) 334, 1198
SfaNI GCATC 3 cut(s) 243, 360, 1032
SfcI CTRYAG 1 cut(s) 792
Sfr274I CTCGAG 3 cut(s) 77, 263, 375
SgrAI CRCCGGYG 1 cut(s) 929
SlaI CTCGAG 3 cut(s) 77, 263, 375
SmiMI CAYNNNNRTG 1 cut(s) 1172
SmlI CTYRAG 4 cut(s) 77, 263, 375, 578
SmoI CTYRAG 4 cut(s) 77, 263, 375, 578
Sse9I AATT 5 cut(s) 198, 827, 898, 997, 1327
SspMI CTAG 2 cut(s) 171, 1184
StyD4I CCNGG 2 cut(s) 332, 1196
StyI CCWWGG 1 cut(s) 1144
TaaI ACNGT 2 cut(s) 180, 1249
TaiI ACGT 8 cut(s) 24, 45, 327, 548, 687, 701, 810, 849
TasI AATT 5 cut(s) 198, 827, 898, 997, 1327
TauI GCSGC 7 cut(s) 8, 218, 245, 305, 351, 362, 453
TfiI GAWTC 1 cut(s) 924
Tru1I TTAA 4 cut(s) 383, 579, 672, 1209
Tru9I TTAA 4 cut(s) 383, 579, 672, 1209
TscAI CASTG 1 cut(s) 232
TseFI GTSAC 1 cut(s) 1113
TseI GCWGC 4 cut(s) 556, 692, 791, 794
Tsp45I GTSAC 1 cut(s) 1113
TspDTI ATGAA 1 cut(s) 791
TspGWI ACGGA 2 cut(s) 61, 822
TspRI CASTG 1 cut(s) 232
Vha464I CTTAAG 1 cut(s) 578
XapI RAATTY 3 cut(s) 198, 827, 898
XceI RCATGY 3 cut(s) 166, 610, 1090
XhoI CTCGAG 3 cut(s) 77, 263, 375
XmiI GTMKAC 2 cut(s) 45, 395
XmnI GAANNNNTTC 2 cut(s) 86, 545
XspI CTAG 2 cut(s) 171, 1184
ZraI GACGTC 2 cut(s) 43, 325
Zsp2I ATGCAT 2 cut(s) 168, 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.