pycom10g20060

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015572
Physical Location & Seq
Reverse (-)
23044568 .. 23046339
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g20060.1

Sequence Viewer

Length: 1338 bp
ATGGCGGCTCCCATGCTCAACATTGACACAAACTCGCTCGACGTCGACAAGCTCTCCTACGAAATCTTCTCGATTCTCGAGAACAACTTCCTCTTCGGAAACCCACCAAGCACAAAACCTCACAATGTCTCCGACCCCAACCCCGCCGATCTATCGACTCCTCTCCACCACATGCATTCTAGCAAACAAGTCACCGGAAAAGTCCGAATCCTCGCCATCGACGCTGCCGGAGCCACCGATGGCATCCTCGCCGCCAACGCGCTCTCCCACCTCGAGTCCTCTCTCCGCCGCAAGTCCGGCGATCCTAGCGCCGCCATCTCCGACTTCTTCGACGTCGTTTCAGGGTCGGGTGCTGGAGGCATTCTCGCTGCCCTCCTCTTCACCCGGGGATTGAAAGACGGGTCGACCCGACCCATGTTCACCGCCAAAGAAGCCCTCGACTTCCTCCTCCAGAACCGCCGCAAGATCTTCAAGTCGTCGCCAGGGGGGATTTTCCAGCGGGTTTTCCGGCCGGCGAAGGCGGAGAAGGAGAGGGAGAAGCTCTTTCGGAAGACTTTCGGGGAGCAGTTGACTCTCAGGGACACGCTGAAGTCGGTGCTGATCCCATGCTACGACATGTCCTCCCGCGCGCCCTTCTTGTTTTCGCGGGCGGACGCGGTGGAGATGGACAGCTACGATTTTAAGATGATGGACGTGTGCGCTGCCACGTCGTCTCAACCTGCCGTTGAGGTGAGGTCGGTGGATTGCAGAACGAAGATCATGGCCGTTGATGGAGGGATCGCAATGAACAATCCGACGGCTGCGGCTATTACGCACGTGCTGAATAATAAGCAGGAGTTTCCCTTCTGTAACGATGTTGAAGATCTGTTAGTGGTGTCGCTTGGAAATGGAGAGTCCCAGTTTGGAAATTTGTCGCCTTCTGGCTGTTTGAGGATCGCCGGTGAAGGAGCTTCAGATTTGGTTGATCAAGCCGTTTCAATGGCATTTGGACAATCCAGAGCAACCAATTATGTTCGCGTGCAAGCCAACGGAATCATATCGAAAATGTATGGAGGCCACCAAAACCCCAACGACAAAAATAAGAACAAAATAGACATGCTGGCCTTGACAGATGAAATGTTGTCACAAAAAAATGTGGAGTCCGTACTATTCCAAGGTAAAAAAATGGTGCAGAGCACAAATGTGGAGAAGCTAGAAATGTTTTCCGGGGAGCTGATTAAGGAACAAGAGAGGAGAAAAACTTGCATTCTGCCAACCGTGGCGTTGAAGCAGACTTCTTCATCATCGCCGAGCAGAACATCATCCGCCACAACCCTATCTATGTCATCTTCGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

446

Amino Acids

48.29

Weight (kDa)

8.08

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 72 - 270 8e-13 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015572)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11650
malus_domestica MD05G1261500.v1.1
prunus_persica Prupe.4G104000_v2.0.a1
pyrus_communis pycom05g24510 pycom10g20060
rosa_chinensis RchiOBHm_Chr5g0018711
rosa_laevigata RLG00000032389
rosa_multiflora Rmu_co8494637.1_g000001
rosa_roxburghii Rroxscaffold_1G00059070 Rroxscaffold_1G00073800
rosa_rugosa Rorug05G0046100
rosa_samantha Rh5AG137100 Rh5BG135900 Rh5CG147900 Rh5DG137000
rosa_wichuraiana Rw5G012150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 45, 336
Acc36I ACCTGC 1 cut(s) 727
AccI GTMKAC 2 cut(s) 45, 404
AccII CGCG 6 cut(s) 260, 627, 629, 646, 656, 1017
AclWI GGATC 4 cut(s) 296, 595, 785, 941
AcoI YGGCCR 2 cut(s) 509, 762
AcsI RAATTY 1 cut(s) 907
AcuI CTGAAG 2 cut(s) 608, 936
AcvI CACGTG 1 cut(s) 817
AcyI GRCGYC 2 cut(s) 42, 333
AfaI GTAC 1 cut(s) 1146
AflIII ACRYGT 2 cut(s) 615, 693
AgsI TTSAA 5 cut(s) 394, 472, 860, 978, 1267
AjiI CACGTC 2 cut(s) 694, 708
AjnI CCWGG 1 cut(s) 481
AleI CACNNNNGTG 1 cut(s) 1181
AluBI AGCT 6 cut(s) 52, 541, 672, 950, 1192, 1213
AluI AGCT 6 cut(s) 52, 541, 672, 950, 1192, 1213
Alw21I GWGCWC 1 cut(s) 1178
Alw26I GTCTC 2 cut(s) 133, 717
AlwI GGATC 4 cut(s) 296, 595, 785, 941
Ama87I CYCGRG 3 cut(s) 77, 272, 384
AoxI GGCC 4 cut(s) 509, 762, 1054, 1101
ApeKI GCWGC 4 cut(s) 224, 368, 701, 800
ApoI RAATTY 1 cut(s) 907
Asp700I GAANNNNTTC 2 cut(s) 86, 554
AspLEI GCGC 5 cut(s) 262, 311, 629, 631, 701
AsuC2I CCSGG 3 cut(s) 385, 386, 1207
AsuHPI GGTGA 5 cut(s) 184, 373, 412, 742, 953
AvaI CYCGRG 3 cut(s) 77, 272, 384
BbrPI CACGTG 1 cut(s) 817
BbsI GAAGAC 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 1178
BbvI GCAGC 4 cut(s) 211, 355, 688, 787
BccI CCATC 6 cut(s) 224, 233, 323, 658, 682, 764
BceAI ACGGC 4 cut(s) 707, 749, 813, 956
BciT130I CCWGG 1 cut(s) 483
BclI TGATCA 1 cut(s) 964
BcnI CCSGG 3 cut(s) 385, 386, 1207
BcoDI GTCTC 2 cut(s) 133, 717
BfaI CTAG 3 cut(s) 180, 306, 1193
BfoI RGCGCY 1 cut(s) 312
BfuAI ACCTGC 1 cut(s) 727
BglII AGATCT 2 cut(s) 465, 862
Bme1390I CCNGG 4 cut(s) 385, 386, 483, 1207
BmeT110I CYCGRG 3 cut(s) 77, 272, 384
BmgBI CACGTC 2 cut(s) 694, 708
BmiI GGNNCC 2 cut(s) 9, 232
BmrFI CCNGG 4 cut(s) 385, 386, 483, 1207
BmrI ACTGGG 1 cut(s) 892
BmsI GCATC 1 cut(s) 252
BmuI ACTGGG 1 cut(s) 892
BpiI GAAGAC 1 cut(s) 557
BplI GAGNNNNNCTC 2 cut(s) 348, 380
BpmI CTGGAG 2 cut(s) 375, 434
BpuMI CCSGG 3 cut(s) 385, 386, 1207
BsaAI YACGTR 1 cut(s) 817
BsaHI GRCGYC 2 cut(s) 42, 333
BsaJI CCNNGG 6 cut(s) 384, 385, 482, 1153, 1206, 1257
BsaWI WCCGGW 1 cut(s) 194
BsaXI ACNNNNNCTCC 8 cut(s) 38, 68, 113, 143, 248, 278, 605, 635
Bse118I RCCGGY 2 cut(s) 511, 938
Bse1I ACTGG 1 cut(s) 898
Bse3DI GCAATG 1 cut(s) 789
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 6 cut(s) 384, 385, 482, 1153, 1206, 1257
BseGI GGATG 2 cut(s) 243, 1301
BseMI GCAATG 1 cut(s) 789
BseMII CTCAG 1 cut(s) 589
BseNI ACTGG 1 cut(s) 898
BsePI GCGCGC 1 cut(s) 627
BseRI GAGGAG 4 cut(s) 150, 365, 437, 1246
BseX3I CGGCCG 1 cut(s) 509
BseXI GCAGC 4 cut(s) 211, 355, 688, 787
BsgI GTGCAG 1 cut(s) 1190
Bsh1236I CGCG 6 cut(s) 260, 627, 629, 646, 656, 1017
Bsh1285I CGRYCG 1 cut(s) 512
BshFI GGCC 4 cut(s) 511, 764, 1056, 1103
BsiEI CGRYCG 1 cut(s) 512
BsiHKAI GWGCWC 1 cut(s) 1178
BsiHKCI CYCGRG 3 cut(s) 77, 272, 384
BsiSI CCGG 8 cut(s) 195, 228, 297, 385, 508, 512, 939, 1206
BslFI GGGAC 2 cut(s) 593, 880
BsmAI GTCTC 2 cut(s) 133, 717
BsmBI CGTCTC 1 cut(s) 717
BsmFI GGGAC 2 cut(s) 593, 880
BsmI GAATGC 3 cut(s) 175, 360, 1245
BsnI GGCC 4 cut(s) 511, 764, 1056, 1103
BsoBI CYCGRG 3 cut(s) 77, 272, 384
Bsp1286I GDGCHC 1 cut(s) 1178
Bsp143I GATC 9 cut(s) 148, 301, 465, 600, 756, 777, 862, 933, 964
BspANI GGCC 4 cut(s) 511, 764, 1056, 1103
BspCNI CTCAG 1 cut(s) 588
BspFNI CGCG 6 cut(s) 260, 627, 629, 646, 656, 1017
BspLI GGNNCC 2 cut(s) 9, 232
BspMI ACCTGC 1 cut(s) 727
BspPI GGATC 4 cut(s) 296, 595, 785, 941
BsrDI GCAATG 1 cut(s) 789
BsrFI RCCGGY 2 cut(s) 511, 938
BsrI ACTGG 1 cut(s) 898
BssAI RCCGGY 2 cut(s) 511, 938
BssECI CCNNGG 6 cut(s) 384, 385, 482, 1153, 1206, 1257
BssHII GCGCGC 1 cut(s) 627
BssMI GATC 9 cut(s) 148, 301, 465, 600, 756, 777, 862, 933, 964
BssNI GRCGYC 2 cut(s) 42, 333
BssT1I CCWWGG 1 cut(s) 1153
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 1 cut(s) 1258
Bst6I CTCTTC 2 cut(s) 98, 383
BstACI GRCGYC 2 cut(s) 42, 333
BstBAI YACGTR 1 cut(s) 817
BstC8I GCNNGC 6 cut(s) 513, 629, 648, 1019, 1023, 1101
BstDEI CTNAG 1 cut(s) 575
BstDSI CCRYGG 1 cut(s) 1257
BstF5I GGATG 2 cut(s) 243, 1301
BstFNI CGCG 6 cut(s) 260, 627, 629, 646, 656, 1017
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 5 cut(s) 262, 311, 629, 631, 701
BstKTI GATC 9 cut(s) 151, 304, 468, 603, 759, 780, 865, 936, 967
BstMAI GTCTC 2 cut(s) 133, 717
BstMBI GATC 9 cut(s) 148, 301, 465, 600, 756, 777, 862, 933, 964
BstMCI CGRYCG 1 cut(s) 512
BstMWI GCNNNNNNNGC 6 cut(s) 221, 230, 257, 297, 306, 431
BstNI CCWGG 1 cut(s) 483
BstNSI RCATGY 3 cut(s) 175, 619, 1099
BstSCI CCNGG 4 cut(s) 383, 384, 481, 1205
BstUI CGCG 6 cut(s) 260, 627, 629, 646, 656, 1017
BstV1I GCAGC 4 cut(s) 211, 355, 688, 787
BstV2I GAAGAC 1 cut(s) 557
BstX2I RGATCY 2 cut(s) 465, 862
BstYI RGATCY 2 cut(s) 465, 862
BstZI CGGCCG 1 cut(s) 509
BsuRI GGCC 4 cut(s) 511, 764, 1056, 1103
BtgI CCRYGG 1 cut(s) 1257
BtgZI GCGATG 1 cut(s) 1269
BtrI CACGTC 2 cut(s) 694, 708
BtsCI GGATG 2 cut(s) 243, 1301
BveI ACCTGC 1 cut(s) 727
Cac8I GCNNGC 6 cut(s) 513, 629, 648, 1019, 1023, 1101
CfoI GCGC 5 cut(s) 262, 311, 629, 631, 701
Cfr10I RCCGGY 2 cut(s) 511, 938
Cfr9I CCCGGG 1 cut(s) 384
CseI GACGC 2 cut(s) 230, 662
Csp6I GTAC 1 cut(s) 1145
CviAII CATG 7 cut(s) 13, 172, 415, 606, 616, 760, 1096
CviQI GTAC 1 cut(s) 1145
DdeI CTNAG 1 cut(s) 575
DpnI GATC 9 cut(s) 150, 303, 467, 602, 758, 779, 864, 935, 966
DpnII GATC 9 cut(s) 148, 301, 465, 600, 756, 777, 862, 933, 964
EaeI YGGCCR 2 cut(s) 509, 762
EagI CGGCCG 1 cut(s) 509
Eam1104I CTCTTC 2 cut(s) 98, 383
EarI CTCTTC 2 cut(s) 98, 383
EciI GGCGGA 4 cut(s) 275, 536, 665, 1294
EclXI CGGCCG 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 1153
Eco52I CGGCCG 1 cut(s) 509
Eco57I CTGAAG 2 cut(s) 608, 936
Eco72I CACGTG 1 cut(s) 817
Eco88I CYCGRG 3 cut(s) 77, 272, 384
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 1 cut(s) 1153
EcoT22I ATGCAT 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 1153
Esp3I CGTCTC 1 cut(s) 717
FaeI CATG 7 cut(s) 16, 175, 418, 609, 619, 763, 1099
FaqI GGGAC 2 cut(s) 593, 880
FatI CATG 7 cut(s) 12, 171, 414, 605, 615, 759, 1095
FauI CCCGC 4 cut(s) 151, 492, 632, 639
FbaI TGATCA 1 cut(s) 964
FblI GTMKAC 2 cut(s) 45, 404
FokI GGATG 2 cut(s) 230, 1288
FspBI CTAG 3 cut(s) 180, 306, 1193
GlaI GCGC 5 cut(s) 261, 310, 628, 630, 700
GsuI CTGGAG 2 cut(s) 375, 434
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 4 cut(s) 511, 764, 1056, 1103
HapII CCGG 8 cut(s) 195, 228, 297, 385, 508, 512, 939, 1206
HgaI GACGC 2 cut(s) 230, 662
HhaI GCGC 5 cut(s) 262, 311, 629, 631, 701
Hin1I GRCGYC 2 cut(s) 42, 333
Hin1II CATG 7 cut(s) 16, 175, 418, 609, 619, 763, 1099
Hin6I GCGC 5 cut(s) 260, 309, 627, 629, 699
HinP1I GCGC 5 cut(s) 260, 309, 627, 629, 699
HincII GTYRAC 3 cut(s) 46, 405, 570
HindII GTYRAC 3 cut(s) 46, 405, 570
HinfI GANTC 8 cut(s) 73, 157, 207, 275, 571, 893, 1032, 1139
HpaII CCGG 8 cut(s) 195, 228, 297, 385, 508, 512, 939, 1206
HphI GGTGA 5 cut(s) 184, 373, 412, 742, 953
Hpy166II GTNNAC 4 cut(s) 46, 405, 420, 570
Hpy188I TCNGA 7 cut(s) 98, 133, 206, 322, 549, 795, 955
Hpy188III TCNNGA 5 cut(s) 70, 77, 79, 451, 996
Hpy8I GTNNAC 4 cut(s) 46, 405, 420, 570
Hpy99I CGWCG 8 cut(s) 44, 47, 224, 335, 338, 481, 712, 799
HpyAV CCTTC 6 cut(s) 511, 520, 643, 853, 927, 938
HpyCH4III ACNGT 1 cut(s) 1258
HpyCH4IV ACGT 5 cut(s) 42, 333, 693, 707, 816
HpyCH4V TGCA 5 cut(s) 175, 747, 1021, 1171, 1245
HpyF10VI GCNNNNNNNGC 6 cut(s) 221, 230, 257, 297, 306, 431
HpyF3I CTNAG 1 cut(s) 575
HpySE526I ACGT 5 cut(s) 42, 333, 693, 707, 816
Hsp92I GRCGYC 2 cut(s) 42, 333
Hsp92II CATG 7 cut(s) 16, 175, 418, 609, 619, 763, 1099
HspAI GCGC 5 cut(s) 260, 309, 627, 629, 699
KroI GCCGGC 1 cut(s) 511
KroNI GCCGGC 1 cut(s) 513
Ksp22I TGATCA 1 cut(s) 964
Kzo9I GATC 9 cut(s) 148, 301, 465, 600, 756, 777, 862, 933, 964
LmnI GCTCC 5 cut(s) 13, 230, 562, 947, 1210
Lsp1109I GCAGC 4 cut(s) 211, 355, 688, 787
LweI GCATC 1 cut(s) 252
MaeI CTAG 3 cut(s) 180, 306, 1193
MaeII ACGT 5 cut(s) 42, 333, 693, 707, 816
MaeIII GTNAC 3 cut(s) 190, 848, 1122
MalI GATC 9 cut(s) 150, 303, 467, 602, 758, 779, 864, 935, 966
MboI GATC 9 cut(s) 148, 301, 465, 600, 756, 777, 862, 933, 964
MflI RGATCY 2 cut(s) 465, 862
MhlI GDGCHC 1 cut(s) 1178
MluCI AATT 2 cut(s) 907, 1006
MlyI GAGTC 5 cut(s) 151, 284, 565, 902, 1148
MmeI TCCRAC 3 cut(s) 156, 345, 818
Mph1103I ATGCAT 1 cut(s) 177
MroNI GCCGGC 1 cut(s) 511
MroXI GAANNNNTTC 2 cut(s) 86, 554
MseI TTAA 2 cut(s) 681, 1218
MslI CAYNNNNRTG 2 cut(s) 1181, 1330
MspA1I CMGCKG 1 cut(s) 499
MspI CCGG 8 cut(s) 195, 228, 297, 385, 508, 512, 939, 1206
MspR9I CCNGG 4 cut(s) 385, 386, 483, 1207
Mva1269I GAATGC 3 cut(s) 175, 360, 1245
MvaI CCWGG 1 cut(s) 483
MvnI CGCG 6 cut(s) 260, 627, 629, 646, 656, 1017
MwoI GCNNNNNNNGC 6 cut(s) 221, 230, 257, 297, 306, 431
NaeI GCCGGC 1 cut(s) 513
NciI CCSGG 3 cut(s) 385, 386, 1207
NdeII GATC 9 cut(s) 148, 301, 465, 600, 756, 777, 862, 933, 964
NgoMIV GCCGGC 1 cut(s) 511
NlaIII CATG 7 cut(s) 16, 175, 418, 609, 619, 763, 1099
NlaIV GGNNCC 2 cut(s) 9, 232
NmeAIII GCCGAG 1 cut(s) 1314
NmuCI GTSAC 2 cut(s) 190, 1122
NsiI ATGCAT 1 cut(s) 177
NspI RCATGY 3 cut(s) 175, 619, 1099
OliI CACNNNNGTG 1 cut(s) 1181
PaeR7I CTCGAG 2 cut(s) 77, 272
PauI GCGCGC 1 cut(s) 627
PciI ACATGT 1 cut(s) 615
PctI GAATGC 3 cut(s) 175, 360, 1245
PdiI GCCGGC 1 cut(s) 513
PdmI GAANNNNTTC 2 cut(s) 86, 554
PfeI GAWTC 3 cut(s) 73, 207, 1032
PleI GAGTC 5 cut(s) 151, 283, 565, 901, 1147
PmaCI CACGTG 1 cut(s) 817
PmlI CACGTG 1 cut(s) 817
PpsI GAGTC 5 cut(s) 151, 283, 565, 901, 1147
Ppu21I YACGTR 1 cut(s) 817
PscI ACATGT 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 481
PspCI CACGTG 1 cut(s) 817
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 2 cut(s) 9, 232
PspXI VCTCGAGB 1 cut(s) 272
PsuI RGATCY 2 cut(s) 465, 862
PteI GCGCGC 1 cut(s) 627
RsaI GTAC 1 cut(s) 1146
RsaNI GTAC 1 cut(s) 1145
RseI CAYNNNNRTG 2 cut(s) 1181, 1330
SalI GTCGAC 2 cut(s) 44, 403
SaqAI TTAA 2 cut(s) 681, 1218
Sau3AI GATC 9 cut(s) 148, 301, 465, 600, 756, 777, 862, 933, 964
SchI GAGTC 5 cut(s) 151, 284, 565, 902, 1148
ScrFI CCNGG 4 cut(s) 385, 386, 483, 1207
SduI GDGCHC 1 cut(s) 1178
SfaNI GCATC 1 cut(s) 252
Sfr274I CTCGAG 2 cut(s) 77, 272
SgrAI CRCCGGYG 1 cut(s) 938
SlaI CTCGAG 2 cut(s) 77, 272
SmaI CCCGGG 1 cut(s) 386
SmiMI CAYNNNNRTG 2 cut(s) 1181, 1330
SmlI CTYRAG 2 cut(s) 77, 272
SmoI CTYRAG 2 cut(s) 77, 272
Sse9I AATT 2 cut(s) 907, 1006
SspMI CTAG 3 cut(s) 180, 306, 1193
StyD4I CCNGG 4 cut(s) 383, 384, 481, 1205
StyI CCWWGG 1 cut(s) 1153
TaaI ACNGT 1 cut(s) 1258
TaiI ACGT 5 cut(s) 45, 336, 696, 710, 819
TasI AATT 2 cut(s) 907, 1006
TauI GCSGC 6 cut(s) 8, 254, 291, 314, 462, 806
TfiI GAWTC 3 cut(s) 73, 207, 1032
Tru1I TTAA 2 cut(s) 681, 1218
Tru9I TTAA 2 cut(s) 681, 1218
TseFI GTSAC 2 cut(s) 190, 1122
TseI GCWGC 4 cut(s) 224, 368, 701, 800
Tsp45I GTSAC 2 cut(s) 190, 1122
TspDTI ATGAA 3 cut(s) 800, 1128, 1269
TspGWI ACGGA 2 cut(s) 1044, 1132
TspMI CCCGGG 1 cut(s) 384
XapI RAATTY 1 cut(s) 907
XceI RCATGY 3 cut(s) 175, 619, 1099
XhoI CTCGAG 2 cut(s) 77, 272
XmaI CCCGGG 1 cut(s) 384
XmiI GTMKAC 2 cut(s) 45, 404
XmnI GAANNNNTTC 2 cut(s) 86, 554
XspI CTAG 3 cut(s) 180, 306, 1193
ZraI GACGTC 2 cut(s) 43, 334
Zsp2I ATGCAT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.