Rw5G012150

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015572
Physical Location & Seq
Forward (+)
14239676 .. 14241895
2220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012150.1

Sequence Viewer

Length: 1518 bp
ATGTTCCACGATCCTCACCACAACATTCGTCTACATACATTTGCGAGTATTCTTACACGCCCTCACACGTTGCTTCTCAACCCATCATCTTCATCAACAATCTTCTACTACAAAAAATTCAACTCTAAGCTCTCACCTACTACTAAACAAACCAAAGCTCTCCAGTACTTCTATTCGTACGCAGCAGCGCTATCCACATCTCCACTGTTCAACATAGACTCGAACTCCGTCGACGTTGACAAGCTCAGCCACGAGATCTTCTCCATTCTCGAGAACAAGTTCCTTTTCGGTTACGACATTGATAACTCCAAAGACACTACTGCTACCACGCCTCTTCATCACATGAAGTCTTGCAAGCACATCACCGGCAAGGTCAGAATCTTAACCATCGACGGCGCCGGAGCCACCGACGGGGTTTTGGCAGCCAAGTCTCTCGTCCACCTCGAGTCCTGCATCCGCCGCAAGTCCGGCGACTCCACTGCCGCCGTTTCTGACTACTTTGATGTTGTTTCTGGCTCCGGCGTCGGCGGCATTCTCGCGGCTCTGCTCTTCACCAAGGCAGCCGACGGATCGGGCCGGCCGGTGTTCACTGCTCAGGAGGCACTCAACTTCCTTTTGGAAAACCGAAGTAAGATTTTCAAGTCGTCTACGGCAGGAATATTCCGGCGAGTTTTCCGGCCGGCGAAGGCTGAGCAGGAGAGGGAGAGGCTCTTCCGCAAGACGTTCGGCGATCAGCTGACGATGAAGGACACCCTTAAGTCGGTGTTGATTCCCTGCTACGACATGTCGTCCCGCGCGCCTTTTCTGTTTTCGCGCGCCGATGCTGTGGAGATGGACGGCTACGATTTCAAGATGAAGGACGTGTGCACCGCCACGTGCGCGCATCCGGCGGCGACGGGGGCCGTGGAGGTTAAGTCAATCGACGGGAGGACGAAGATCATGGCCGTCGACGGCGGAATTGCCATGAACAATCCGACGGCCGCCGCTATTACGCACGTTCTGAATAATAAGCAAGAGTTTCCGTTCTGTTCTGGTGTGGAGGATCTCTTGGTGGTGTCACTCGGAAATGGAGAGTCGGAGTTCGGAAACTTGTCGCCTTCTGGCTGCCTAAGAATCGCCGGCGAAGGAGCTTCAGACTTGGTTGATCAAGCTGTTTCAATGGCATTTGGGCAATCCCGGACCAGCAATTATGTTCGCGTGCAAGCCACCGGCATCATAGCCAAAAACCCTGCGCGCATGGACCACTCCAACAACAAGAACAACACCAATACAGACATATTGGCTTTAACAGAGGAAATGTTGGCACAGAGAAACGTGGAGTCGATTTTATTCGAGGGGAAGAAAATGGTGGAGAGCACGAATTTGAAGAAGTTGGAGTTGTTCGCCGGAGAGGTGATCAAGGAAGAAGAGAGGAGAAAGACCAGCATTTTGCCGACGGTGGCTTTGAAGCAGACTTCGGGGTCGCCAGCCAGAACATCGTCTGCTACTACCTTATCGAGTCTGTCATCTTCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

505

Amino Acids

54.92

Weight (kDa)

8.73

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 132 - 330 6.2e-14 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015572)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11650
malus_domestica MD05G1261500.v1.1
prunus_persica Prupe.4G104000_v2.0.a1
pyrus_communis pycom05g24510 pycom10g20060
rosa_chinensis RchiOBHm_Chr5g0018711
rosa_laevigata RLG00000032389
rosa_multiflora Rmu_co8494637.1_g000001
rosa_roxburghii Rroxscaffold_1G00059070 Rroxscaffold_1G00073800
rosa_rugosa Rorug05G0046100
rosa_samantha Rh5AG137100 Rh5BG135900 Rh5CG147900 Rh5DG137000
rosa_wichuraiana Rw5G012150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1459
AccB1I GGYRCC 1 cut(s) 395
AccI GTMKAC 4 cut(s) 31, 231, 647, 948
AccII CGCG 8 cut(s) 539, 795, 797, 814, 816, 881, 1197, 1234
AclWI GGATC 3 cut(s) 5, 577, 1050
AcoI YGGCCR 4 cut(s) 578, 677, 942, 978
AcsI RAATTY 2 cut(s) 116, 1360
AcuI CTGAAG 1 cut(s) 1116
AcvI CACGTG 1 cut(s) 876
AcyI GRCGYC 2 cut(s) 396, 522
AfaI GTAC 2 cut(s) 167, 179
AfeI AGCGCT 1 cut(s) 189
AfiI CCNNNNNNNGG 2 cut(s) 411, 760
AflII CTTAAG 1 cut(s) 755
AflIII ACRYGT 3 cut(s) 66, 783, 861
AgsI TTSAA 7 cut(s) 121, 211, 640, 850, 1158, 1366, 1447
AhdI GACNNNNNGTC 1 cut(s) 787
AjiI CACGTC 1 cut(s) 862
AluBI AGCT 6 cut(s) 130, 158, 244, 736, 1130, 1151
AluI AGCT 6 cut(s) 130, 158, 244, 736, 1130, 1151
Alw21I GWGCWC 2 cut(s) 869, 1358
Alw26I GTCTC 1 cut(s) 435
Alw44I GTGCAC 1 cut(s) 865
AlwI GGATC 3 cut(s) 5, 577, 1050
Ama87I CYCGRG 2 cut(s) 269, 443
Aor51HI AGCGCT 1 cut(s) 189
AoxI GGCC 6 cut(s) 574, 578, 677, 900, 942, 978
ApaLI GTGCAC 1 cut(s) 865
ApeKI GCWGC 5 cut(s) 182, 185, 422, 560, 1104
ApoI RAATTY 2 cut(s) 116, 1360
ArsI GACNNNNNNTTYG 4 cut(s) 1411, 1426, 1443, 1458
Asp700I GAANNNNTTC 1 cut(s) 278
AspS9I GGNCC 4 cut(s) 574, 900, 1179, 1240
AsuC2I CCSGG 1 cut(s) 1177
AsuHPI GGTGA 5 cut(s) 8, 126, 355, 544, 1405
AvaI CYCGRG 2 cut(s) 269, 443
AvaII GGWCC 2 cut(s) 1179, 1240
BaeGI GKGCMC 1 cut(s) 869
BanI GGYRCC 1 cut(s) 395
BauI CACGAG 1 cut(s) 251
BbrPI CACGTG 1 cut(s) 876
Bbv12I GWGCWC 2 cut(s) 869, 1358
BbvI GCAGC 5 cut(s) 194, 197, 434, 572, 1091
BccI CCATC 3 cut(s) 91, 395, 826
BceAI ACGGC 8 cut(s) 409, 470, 666, 853, 887, 929, 967, 993
BcgI CGANNNNNNTGC 4 cut(s) 461, 495, 706, 740
BclI TGATCA 2 cut(s) 1144, 1395
BcnI CCSGG 1 cut(s) 1177
BcoDI GTCTC 1 cut(s) 435
BfoI RGCGCY 2 cut(s) 191, 399
BfrI CTTAAG 1 cut(s) 755
BglII AGATCT 1 cut(s) 255
BlpI GCTNAGC 2 cut(s) 245, 690
BmcAI AGTACT 1 cut(s) 167
Bme1390I CCNGG 1 cut(s) 1177
Bme18I GGWCC 2 cut(s) 1179, 1240
BmeRI GACNNNNNGTC 1 cut(s) 787
BmeT110I CYCGRG 2 cut(s) 269, 443
BmgBI CACGTC 1 cut(s) 862
BmgT120I GGNCC 4 cut(s) 574, 900, 1179, 1240
BmiI GGNNCC 4 cut(s) 397, 403, 517, 901
BmrFI CCNGG 1 cut(s) 1177
BmsI GCATC 4 cut(s) 462, 811, 892, 1221
BplI GAGNNNNNCTC 2 cut(s) 245, 277
BpmI CTGGAG 1 cut(s) 146
Bpu10I CCTNAGC 1 cut(s) 594
Bpu1102I GCTNAGC 2 cut(s) 245, 690
BpuMI CCSGG 1 cut(s) 1177
BsaAI YACGTR 1 cut(s) 876
BsaHI GRCGYC 2 cut(s) 396, 522
BsaJI CCNNGG 2 cut(s) 555, 903
BsaXI ACNNNNNCTCC 4 cut(s) 209, 239, 899, 929
Bsc4I CCNNNNNNNGG 2 cut(s) 411, 760
Bse118I RCCGGY 6 cut(s) 365, 576, 580, 679, 1118, 1208
Bse1I ACTGG 1 cut(s) 163
BseDI CCNNGG 2 cut(s) 555, 903
BseGI GGATG 2 cut(s) 453, 883
BseLI CCNNNNNNNGG 2 cut(s) 411, 760
BseMII CTCAG 3 cut(s) 259, 608, 681
BseNI ACTGG 1 cut(s) 163
BsePI GCGCGC 4 cut(s) 795, 814, 879, 1232
BseRI GAGGAG 1 cut(s) 1426
BseSI GKGCMC 1 cut(s) 869
BseX3I CGGCCG 3 cut(s) 578, 677, 978
BseXI GCAGC 5 cut(s) 194, 197, 434, 572, 1091
Bsh1236I CGCG 8 cut(s) 539, 795, 797, 814, 816, 881, 1197, 1234
Bsh1285I CGRYCG 3 cut(s) 581, 680, 981
BshFI GGCC 6 cut(s) 576, 580, 679, 902, 944, 980
BshNI GGYRCC 1 cut(s) 395
BsiEI CGRYCG 3 cut(s) 581, 680, 981
BsiHKAI GWGCWC 2 cut(s) 869, 1358
BsiHKCI CYCGRG 2 cut(s) 269, 443
BsiWI CGTACG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 775
BslI CCNNNNNNNGG 2 cut(s) 411, 760
BsmAI GTCTC 1 cut(s) 435
BsmFI GGGAC 1 cut(s) 775
BsmI GAATGC 1 cut(s) 531
BsnI GGCC 6 cut(s) 576, 580, 679, 902, 944, 980
BsoBI CYCGRG 2 cut(s) 269, 443
Bsp1286I GDGCHC 2 cut(s) 869, 1358
Bsp143I GATC 8 cut(s) 10, 255, 569, 730, 936, 1042, 1144, 1395
Bsp1720I GCTNAGC 2 cut(s) 245, 690
BspANI GGCC 6 cut(s) 576, 580, 679, 902, 944, 980
BspCNI CTCAG 3 cut(s) 258, 607, 682
BspFNI CGCG 8 cut(s) 539, 795, 797, 814, 816, 881, 1197, 1234
BspLI GGNNCC 4 cut(s) 397, 403, 517, 901
BspPI GGATC 3 cut(s) 5, 577, 1050
BspQI GCTCTTC 2 cut(s) 554, 716
BspT107I GGYRCC 1 cut(s) 395
BspTI CTTAAG 1 cut(s) 755
BsrFI RCCGGY 6 cut(s) 365, 576, 580, 679, 1118, 1208
BsrI ACTGG 1 cut(s) 163
BssAI RCCGGY 6 cut(s) 365, 576, 580, 679, 1118, 1208
BssECI CCNNGG 2 cut(s) 555, 903
BssHII GCGCGC 4 cut(s) 795, 814, 879, 1232
BssMI GATC 8 cut(s) 10, 255, 569, 730, 936, 1042, 1144, 1395
BssNI GRCGYC 2 cut(s) 396, 522
BssSI CACGAG 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 555
Bst2BI CACGAG 1 cut(s) 251
Bst4CI ACNGT 2 cut(s) 207, 1438
Bst6I CTCTTC 4 cut(s) 339, 554, 716, 1401
BstACI GRCGYC 2 cut(s) 396, 522
BstAFI CTTAAG 1 cut(s) 755
BstBAI YACGTR 1 cut(s) 876
BstDEI CTNAG 5 cut(s) 126, 245, 594, 690, 1109
BstDSI CCRYGG 1 cut(s) 903
BstF5I GGATG 2 cut(s) 453, 883
BstFNI CGCG 8 cut(s) 539, 795, 797, 814, 816, 881, 1197, 1234
BstH2I RGCGCY 2 cut(s) 191, 399
BstKTI GATC 8 cut(s) 13, 258, 572, 733, 939, 1045, 1147, 1398
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 8 cut(s) 10, 255, 569, 730, 936, 1042, 1144, 1395
BstMCI CGRYCG 3 cut(s) 581, 680, 981
BstMWI GCNNNNNNNGC 7 cut(s) 459, 468, 528, 599, 878, 887, 899
BstNSI RCATGY 1 cut(s) 787
BstSCI CCNGG 1 cut(s) 1175
BstSLI GKGCMC 1 cut(s) 869
BstUI CGCG 8 cut(s) 539, 795, 797, 814, 816, 881, 1197, 1234
BstV1I GCAGC 5 cut(s) 194, 197, 434, 572, 1091
BstX2I RGATCY 2 cut(s) 255, 1042
BstYI RGATCY 2 cut(s) 255, 1042
BstZI CGGCCG 3 cut(s) 578, 677, 978
BsuRI GGCC 6 cut(s) 576, 580, 679, 902, 944, 980
BtgI CCRYGG 1 cut(s) 903
BtrI CACGTC 1 cut(s) 862
BtsCI GGATG 2 cut(s) 453, 883
BtsI GCAGTG 2 cut(s) 477, 588
BtsIMutI CAGTG 3 cut(s) 203, 477, 588
Cfr10I RCCGGY 6 cut(s) 365, 576, 580, 679, 1118, 1208
Cfr13I GGNCC 4 cut(s) 574, 900, 1179, 1240
CseI GACGC 1 cut(s) 511
Csp6I GTAC 2 cut(s) 166, 178
CviAII CATG 5 cut(s) 343, 784, 940, 964, 1237
CviQI GTAC 2 cut(s) 166, 178
DdeI CTNAG 5 cut(s) 126, 245, 594, 690, 1109
DinI GGCGCC 1 cut(s) 397
DpnI GATC 8 cut(s) 12, 257, 571, 732, 938, 1044, 1146, 1397
DpnII GATC 8 cut(s) 10, 255, 569, 730, 936, 1042, 1144, 1395
DrdI GACNNNNNNGTC 1 cut(s) 1459
DriI GACNNNNNGTC 1 cut(s) 787
DseDI GACNNNNNNGTC 1 cut(s) 1459
EaeI YGGCCR 4 cut(s) 578, 677, 942, 978
EagI CGGCCG 3 cut(s) 578, 677, 978
Eam1104I CTCTTC 4 cut(s) 339, 554, 716, 1401
Eam1105I GACNNNNNGTC 1 cut(s) 787
EarI CTCTTC 4 cut(s) 339, 554, 716, 1401
EciI GGCGGA 2 cut(s) 446, 969
EclXI CGGCCG 3 cut(s) 578, 677, 978
Eco130I CCWWGG 1 cut(s) 555
Eco47I GGWCC 2 cut(s) 1179, 1240
Eco47III AGCGCT 1 cut(s) 189
Eco52I CGGCCG 3 cut(s) 578, 677, 978
Eco57I CTGAAG 1 cut(s) 1116
Eco72I CACGTG 1 cut(s) 876
Eco88I CYCGRG 2 cut(s) 269, 443
EcoT14I CCWWGG 1 cut(s) 555
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 555
FaeI CATG 5 cut(s) 346, 787, 943, 967, 1240
FaqI GGGAC 1 cut(s) 775
FatI CATG 5 cut(s) 342, 783, 939, 963, 1236
FauI CCCGC 1 cut(s) 800
FbaI TGATCA 2 cut(s) 1144, 1395
FblI GTMKAC 4 cut(s) 31, 231, 647, 948
FokI GGATG 2 cut(s) 440, 870
FseI GGCCGGCC 1 cut(s) 580
GsuI CTGGAG 1 cut(s) 146
HaeII RGCGCY 2 cut(s) 191, 399
HaeIII GGCC 6 cut(s) 576, 580, 679, 902, 944, 980
HgaI GACGC 1 cut(s) 511
Hin1I GRCGYC 2 cut(s) 396, 522
Hin1II CATG 5 cut(s) 346, 787, 943, 967, 1240
HincII GTYRAC 3 cut(s) 232, 238, 949
HindII GTYRAC 3 cut(s) 232, 238, 949
HinfI GANTC 9 cut(s) 218, 378, 446, 473, 769, 1073, 1113, 1319, 1498
HphI GGTGA 5 cut(s) 8, 126, 355, 544, 1405
Hpy166II GTNNAC 8 cut(s) 32, 232, 238, 439, 588, 648, 867, 949
Hpy188I TCNGA 8 cut(s) 377, 493, 975, 1002, 1064, 1078, 1085, 1135
Hpy188III TCNNGA 4 cut(s) 269, 271, 596, 850
Hpy8I GTNNAC 8 cut(s) 32, 232, 238, 439, 588, 648, 867, 949
HpyAV CCTTC 5 cut(s) 679, 739, 850, 1107, 1118
HpyCH4III ACNGT 2 cut(s) 207, 1438
HpyCH4IV ACGT 7 cut(s) 68, 234, 722, 861, 875, 996, 1314
HpyCH4V TGCA 4 cut(s) 354, 453, 867, 1201
HpyF10VI GCNNNNNNNGC 7 cut(s) 459, 468, 528, 599, 878, 887, 899
HpyF3I CTNAG 5 cut(s) 126, 245, 594, 690, 1109
HpySE526I ACGT 7 cut(s) 68, 234, 722, 861, 875, 996, 1314
Hsp92I GRCGYC 2 cut(s) 396, 522
Hsp92II CATG 5 cut(s) 346, 787, 943, 967, 1240
KasI GGCGCC 1 cut(s) 395
KroI GCCGGC 3 cut(s) 576, 679, 1118
KroNI GCCGGC 3 cut(s) 578, 681, 1120
Ksp22I TGATCA 2 cut(s) 1144, 1395
Kzo9I GATC 8 cut(s) 10, 255, 569, 730, 936, 1042, 1144, 1395
LguI GCTCTTC 2 cut(s) 554, 716
LmnI GCTCC 3 cut(s) 401, 521, 1127
Lsp1109I GCAGC 5 cut(s) 194, 197, 434, 572, 1091
LweI GCATC 4 cut(s) 462, 811, 892, 1221
MaeII ACGT 7 cut(s) 68, 234, 722, 861, 875, 996, 1314
MaeIII GTNAC 2 cut(s) 290, 1056
MalI GATC 8 cut(s) 12, 257, 571, 732, 938, 1044, 1146, 1397
MboI GATC 8 cut(s) 10, 255, 569, 730, 936, 1042, 1144, 1395
MflI RGATCY 2 cut(s) 255, 1042
MhlI GDGCHC 2 cut(s) 869, 1358
MluCI AATT 4 cut(s) 116, 957, 1186, 1360
Mly113I GGCGCC 1 cut(s) 396
MlyI GAGTC 6 cut(s) 212, 455, 467, 1082, 1328, 1507
MmeI TCCRAC 4 cut(s) 998, 1056, 1272, 1353
MreI CGCCGGCG 1 cut(s) 1118
MroNI GCCGGC 3 cut(s) 576, 679, 1118
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 4 cut(s) 383, 756, 912, 1286
MslI CAYNNNNRTG 1 cut(s) 1510
MspA1I CMGCKG 1 cut(s) 736
MspCI CTTAAG 1 cut(s) 755
MspR9I CCNGG 1 cut(s) 1177
Mva1269I GAATGC 1 cut(s) 531
MvnI CGCG 8 cut(s) 539, 795, 797, 814, 816, 881, 1197, 1234
MwoI GCNNNNNNNGC 7 cut(s) 459, 468, 528, 599, 878, 887, 899
NaeI GCCGGC 3 cut(s) 578, 681, 1120
NarI GGCGCC 1 cut(s) 396
NciI CCSGG 1 cut(s) 1177
NdeII GATC 8 cut(s) 10, 255, 569, 730, 936, 1042, 1144, 1395
NgoMIV GCCGGC 3 cut(s) 576, 679, 1118
NlaIII CATG 5 cut(s) 346, 787, 943, 967, 1240
NlaIV GGNNCC 4 cut(s) 397, 403, 517, 901
NmuCI GTSAC 1 cut(s) 1056
NspI RCATGY 1 cut(s) 787
PaeR7I CTCGAG 2 cut(s) 269, 443
PauI GCGCGC 4 cut(s) 795, 814, 879, 1232
PciI ACATGT 1 cut(s) 783
PciSI GCTCTTC 2 cut(s) 554, 716
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PctI GAATGC 1 cut(s) 531
PdiI GCCGGC 3 cut(s) 578, 681, 1120
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 3 cut(s) 378, 769, 1113
Pfl23II CGTACG 1 cut(s) 177
PfoI TCCNGGA 1 cut(s) 1175
PleI GAGTC 6 cut(s) 212, 454, 467, 1081, 1327, 1506
PluTI GGCGCC 1 cut(s) 399
PmaCI CACGTG 1 cut(s) 876
PmlI CACGTG 1 cut(s) 876
PpsI GAGTC 6 cut(s) 212, 454, 467, 1081, 1327, 1506
Ppu21I YACGTR 1 cut(s) 876
PscI ACATGT 1 cut(s) 783
PspCI CACGTG 1 cut(s) 876
PspLI CGTACG 1 cut(s) 177
PspN4I GGNNCC 4 cut(s) 397, 403, 517, 901
PspPI GGNCC 4 cut(s) 574, 900, 1179, 1240
PspXI VCTCGAGB 1 cut(s) 443
PsuI RGATCY 2 cut(s) 255, 1042
PteI GCGCGC 4 cut(s) 795, 814, 879, 1232
PvuII CAGCTG 1 cut(s) 736
RigI GGCCGGCC 1 cut(s) 580
RsaI GTAC 2 cut(s) 167, 179
RsaNI GTAC 2 cut(s) 166, 178
RseI CAYNNNNRTG 1 cut(s) 1510
SalI GTCGAC 2 cut(s) 230, 947
SapI GCTCTTC 2 cut(s) 554, 716
SaqAI TTAA 4 cut(s) 383, 756, 912, 1286
Sau3AI GATC 8 cut(s) 10, 255, 569, 730, 936, 1042, 1144, 1395
Sau96I GGNCC 4 cut(s) 574, 900, 1179, 1240
ScaI AGTACT 1 cut(s) 167
SchI GAGTC 6 cut(s) 212, 455, 467, 1082, 1328, 1507
ScrFI CCNGG 1 cut(s) 1177
SduI GDGCHC 2 cut(s) 869, 1358
SfaNI GCATC 4 cut(s) 462, 811, 892, 1221
SfoI GGCGCC 1 cut(s) 397
Sfr274I CTCGAG 2 cut(s) 269, 443
SgrAI CRCCGGYG 1 cut(s) 1118
SgrDI CGTCGACG 2 cut(s) 230, 947
SinI GGWCC 2 cut(s) 1179, 1240
SlaI CTCGAG 2 cut(s) 269, 443
SmiMI CAYNNNNRTG 1 cut(s) 1510
SmlI CTYRAG 3 cut(s) 269, 443, 755
SmoI CTYRAG 3 cut(s) 269, 443, 755
Sse9I AATT 4 cut(s) 116, 957, 1186, 1360
SspDI GGCGCC 1 cut(s) 395
SspI AATATT 1 cut(s) 660
StyD4I CCNGG 1 cut(s) 1175
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 2 cut(s) 207, 1438
TaiI ACGT 7 cut(s) 71, 237, 725, 864, 878, 999, 1317
TasI AATT 4 cut(s) 116, 957, 1186, 1360
TatI WGTACW 1 cut(s) 165
TauI GCSGC 7 cut(s) 462, 485, 531, 542, 893, 983, 986
TfiI GAWTC 3 cut(s) 378, 769, 1113
Tru1I TTAA 4 cut(s) 383, 756, 912, 1286
Tru9I TTAA 4 cut(s) 383, 756, 912, 1286
TscAI CASTG 3 cut(s) 210, 484, 595
TseFI GTSAC 1 cut(s) 1056
TseI GCWGC 5 cut(s) 182, 185, 422, 560, 1104
Tsp45I GTSAC 1 cut(s) 1056
TspDTI ATGAA 6 cut(s) 81, 326, 359, 758, 869, 980
TspGWI ACGGA 3 cut(s) 217, 582, 1011
TspRI CASTG 3 cut(s) 210, 484, 595
Vha464I CTTAAG 1 cut(s) 755
VneI GTGCAC 1 cut(s) 865
VpaK11BI GGWCC 2 cut(s) 1179, 1240
XapI RAATTY 2 cut(s) 116, 1360
XceI RCATGY 1 cut(s) 787
XhoI CTCGAG 2 cut(s) 269, 443
XmiI GTMKAC 4 cut(s) 31, 231, 647, 948
XmnI GAANNNNTTC 1 cut(s) 278
ZrmI AGTACT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.