RLG00000032389

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015572
Physical Location & Seq
Forward (+)
14306545 .. 14308494
1950 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032389

Sequence Viewer

Length: 1296 bp
ATGGCGGCAGCAGCAGCACTATCCACATCTCCACTGTTCAACATGGACTCGAACTCCGTCGACGTTGACAAGCTCAGCCACGAGATCTTCTCCATTCTCGAGAACAAGTTCCTCTTCGGTTACGACATTGATAACTCCAAAGACACTACCGCTACCACGCCTCTTCATCACATGAAGTCTAGCAAGCACATCACCGGCAAGGTCAGAATCTTAACCATCGACGGCGCCGGCGCCACCGACGGGATTTTGGCAGCCAAGTCTCTCGTCCACCTCGAGTCCTGCATCCGCCGCAAGTCCGGCGACTCCACTGCCGCCGTTTCTGACTACTTTGATGTTGTTTCTGGCTCCGGCGGCCGGCCGGTGTTCACTGCTCAGGAGGCACTCAACTTCCTTTTGGAAAACCGAAGTAAGATTTTCAAGTCGTCTACTGCAGGAATATTCCGGGGAGTTTTCCGGCCAGCGAAGGCGGAGAAGGAGAGGGAGAGGCTCTTCCGCAAGACGTTCGGCGATCAGCTGACGATGAAGGACACCCTTAAGTCGGTGTTGATTCCCTGCTACGACATGTCGTCCCGCGCGCCTTTTCTGTTTTCGCGCGCCGACGCTGTGGAGATGGACGGCTACGATTTCAAGATGAAGGACGTGTGCACCGCCACGTGCGCGCATCCGGCGGCGACGGGGGCCGTGGAGGTTAAGTCAGTCGACGGGAGGACGAAGATCATGGCCGTCGACGGCGGAATTGCCATGAACAATCCGACGGCCGCGGCTATTACGCACGTTCTGAATAATAAGCAAGAGTTTCCGTTCTGTTCTGGTGTGGAGGATCTCTTGGTGGTGTCACTCGGAAATGGAGAGTCGGAGTTCGGAAACCTGTCTCCTTCTGGCTGCCTAAGAATCGCCGGCGAAGGAGCTTCAGACTTGGTTGATCAAGCTGTTTCAATGGCATTTGGGCAATCCCGGACCAGCAATTATGTTCGCGTGCAAGCCACCGGCATCATAGCCAAAAACCCTGCGCGCATGGACCACTCCAACAACAAGAACAACACCAATACAGACATATTGGATTTAACAGAGGAAATGTTGGCACAGAGAAACGTGGAGTCGATTTTATTCGAGGGGAAGAAAATGGTGGAGAGCACGAATTTGAAGAAGTTGGAGTTGTTCGCCGGAGAGGTGATCAAGGAAGAAGAGAGGAGAAAGACCAGCATTTTGCCGACGGTGGCTTTGAAGCAGACTTCGGGGTCGCCAGCCAGAACATCGTCTGCTACTACCTTATCGAGTCTGTCATCTTCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

432

Amino Acids

46.46

Weight (kDa)

6.73

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 160 - 256 4.5e-07 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015572)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11650
malus_domestica MD05G1261500.v1.1
prunus_persica Prupe.4G104000_v2.0.a1
pyrus_communis pycom05g24510 pycom10g20060
rosa_chinensis RchiOBHm_Chr5g0018711
rosa_laevigata RLG00000032389
rosa_multiflora Rmu_co8494637.1_g000001
rosa_roxburghii Rroxscaffold_1G00059070 Rroxscaffold_1G00073800
rosa_rugosa Rorug05G0046100
rosa_samantha Rh5AG137100 Rh5BG135900 Rh5CG147900 Rh5DG137000
rosa_wichuraiana Rw5G012150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1237
AccB1I GGYRCC 2 cut(s) 224, 230
AccI GTMKAC 4 cut(s) 60, 425, 699, 726
AccII CGCG 8 cut(s) 573, 575, 592, 594, 659, 761, 975, 1012
AclWI GGATC 1 cut(s) 828
AcoI YGGCCR 5 cut(s) 352, 356, 455, 720, 756
AcsI RAATTY 1 cut(s) 1138
AcuI CTGAAG 1 cut(s) 894
AcvI CACGTG 1 cut(s) 654
AcyI GRCGYC 2 cut(s) 225, 231
AfiI CCNNNNNNNGG 3 cut(s) 240, 354, 538
AflII CTTAAG 1 cut(s) 533
AflIII ACRYGT 2 cut(s) 561, 639
AgsI TTSAA 6 cut(s) 40, 418, 628, 936, 1144, 1225
AhdI GACNNNNNGTC 1 cut(s) 565
AjiI CACGTC 1 cut(s) 640
AluBI AGCT 4 cut(s) 73, 514, 908, 929
AluI AGCT 4 cut(s) 73, 514, 908, 929
Alw21I GWGCWC 2 cut(s) 647, 1136
Alw26I GTCTC 2 cut(s) 264, 876
Alw44I GTGCAC 1 cut(s) 643
AlwI GGATC 1 cut(s) 828
Ama87I CYCGRG 2 cut(s) 98, 272
AoxI GGCC 6 cut(s) 352, 356, 455, 678, 720, 756
ApaLI GTGCAC 1 cut(s) 643
ApeKI GCWGC 5 cut(s) 8, 11, 14, 251, 882
ApoI RAATTY 1 cut(s) 1138
ArsI GACNNNNNNTTYG 4 cut(s) 1189, 1204, 1221, 1236
Asp700I GAANNNNTTC 1 cut(s) 107
AspS9I GGNCC 3 cut(s) 678, 957, 1018
AsuC2I CCSGG 2 cut(s) 443, 955
AsuHPI GGTGA 2 cut(s) 184, 1183
AvaI CYCGRG 2 cut(s) 98, 272
AvaII GGWCC 2 cut(s) 957, 1018
BaeGI GKGCMC 1 cut(s) 647
BanI GGYRCC 2 cut(s) 224, 230
BauI CACGAG 1 cut(s) 80
BbrPI CACGTG 1 cut(s) 654
Bbv12I GWGCWC 2 cut(s) 647, 1136
BbvI GCAGC 5 cut(s) 20, 23, 26, 263, 869
BccI CCATC 2 cut(s) 224, 604
BceAI ACGGC 7 cut(s) 238, 299, 631, 665, 707, 745, 771
BcgI CGANNNNNNTGC 4 cut(s) 290, 324, 484, 518
BclI TGATCA 2 cut(s) 922, 1173
BcnI CCSGG 2 cut(s) 443, 955
BcoDI GTCTC 2 cut(s) 264, 876
BfaI CTAG 1 cut(s) 180
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 2 cut(s) 228, 234
BfrI CTTAAG 1 cut(s) 533
BglII AGATCT 1 cut(s) 84
BlpI GCTNAGC 1 cut(s) 74
Bme1390I CCNGG 2 cut(s) 443, 955
Bme18I GGWCC 2 cut(s) 957, 1018
BmeRI GACNNNNNGTC 1 cut(s) 565
BmeT110I CYCGRG 2 cut(s) 98, 272
BmgBI CACGTC 1 cut(s) 640
BmgT120I GGNCC 3 cut(s) 678, 957, 1018
BmiI GGNNCC 4 cut(s) 226, 232, 346, 679
BmrFI CCNGG 2 cut(s) 443, 955
BmsI GCATC 3 cut(s) 291, 670, 999
BplI GAGNNNNNCTC 2 cut(s) 74, 106
Bpu10I CCTNAGC 1 cut(s) 372
Bpu1102I GCTNAGC 1 cut(s) 74
BpuMI CCSGG 2 cut(s) 443, 955
BsaAI YACGTR 1 cut(s) 654
BsaHI GRCGYC 2 cut(s) 225, 231
BsaJI CCNNGG 3 cut(s) 442, 681, 759
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 677, 707
Bsc4I CCNNNNNNNGG 3 cut(s) 240, 354, 538
Bse118I RCCGGY 6 cut(s) 194, 227, 354, 358, 896, 986
BseDI CCNNGG 3 cut(s) 442, 681, 759
BseGI GGATG 2 cut(s) 282, 661
BseLI CCNNNNNNNGG 3 cut(s) 240, 354, 538
BseMII CTCAG 2 cut(s) 88, 386
BsePI GCGCGC 4 cut(s) 573, 592, 657, 1010
BseRI GAGGAG 1 cut(s) 1204
BseSI GKGCMC 1 cut(s) 647
BseX3I CGGCCG 3 cut(s) 352, 356, 756
BseXI GCAGC 5 cut(s) 20, 23, 26, 263, 869
Bsh1236I CGCG 8 cut(s) 573, 575, 592, 594, 659, 761, 975, 1012
Bsh1285I CGRYCG 3 cut(s) 355, 359, 759
BshFI GGCC 6 cut(s) 354, 358, 457, 680, 722, 758
BshNI GGYRCC 2 cut(s) 224, 230
BsiEI CGRYCG 3 cut(s) 355, 359, 759
BsiHKAI GWGCWC 2 cut(s) 647, 1136
BsiHKCI CYCGRG 2 cut(s) 98, 272
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 3 cut(s) 240, 354, 538
BsmAI GTCTC 2 cut(s) 264, 876
BsmFI GGGAC 1 cut(s) 553
BsnI GGCC 6 cut(s) 354, 358, 457, 680, 722, 758
BsoBI CYCGRG 2 cut(s) 98, 272
Bsp1286I GDGCHC 2 cut(s) 647, 1136
Bsp143I GATC 6 cut(s) 84, 508, 714, 820, 922, 1173
Bsp1720I GCTNAGC 1 cut(s) 74
BspANI GGCC 6 cut(s) 354, 358, 457, 680, 722, 758
BspCNI CTCAG 2 cut(s) 87, 385
BspFNI CGCG 8 cut(s) 573, 575, 592, 594, 659, 761, 975, 1012
BspLI GGNNCC 4 cut(s) 226, 232, 346, 679
BspMAI CTGCAG 1 cut(s) 433
BspPI GGATC 1 cut(s) 828
BspQI GCTCTTC 1 cut(s) 494
BspT107I GGYRCC 2 cut(s) 224, 230
BspTI CTTAAG 1 cut(s) 533
BsrFI RCCGGY 6 cut(s) 194, 227, 354, 358, 896, 986
BssAI RCCGGY 6 cut(s) 194, 227, 354, 358, 896, 986
BssECI CCNNGG 3 cut(s) 442, 681, 759
BssHII GCGCGC 4 cut(s) 573, 592, 657, 1010
BssMI GATC 6 cut(s) 84, 508, 714, 820, 922, 1173
BssNI GRCGYC 2 cut(s) 225, 231
BssSI CACGAG 1 cut(s) 80
Bst2BI CACGAG 1 cut(s) 80
Bst4CI ACNGT 2 cut(s) 36, 1216
Bst6I CTCTTC 4 cut(s) 119, 168, 494, 1179
BstACI GRCGYC 2 cut(s) 225, 231
BstAFI CTTAAG 1 cut(s) 533
BstBAI YACGTR 1 cut(s) 654
BstDEI CTNAG 3 cut(s) 74, 372, 887
BstDSI CCRYGG 2 cut(s) 681, 759
BstF5I GGATG 2 cut(s) 282, 661
BstFNI CGCG 8 cut(s) 573, 575, 592, 594, 659, 761, 975, 1012
BstH2I RGCGCY 2 cut(s) 228, 234
BstKTI GATC 6 cut(s) 87, 511, 717, 823, 925, 1176
BstMAI GTCTC 2 cut(s) 264, 876
BstMBI GATC 6 cut(s) 84, 508, 714, 820, 922, 1173
BstMCI CGRYCG 3 cut(s) 355, 359, 759
BstMWI GCNNNNNNNGC 9 cut(s) 11, 14, 288, 297, 351, 377, 656, 665, 677
BstNSI RCATGY 1 cut(s) 565
BstSCI CCNGG 2 cut(s) 441, 953
BstSFI CTRYAG 1 cut(s) 429
BstSLI GKGCMC 1 cut(s) 647
BstUI CGCG 8 cut(s) 573, 575, 592, 594, 659, 761, 975, 1012
BstV1I GCAGC 5 cut(s) 20, 23, 26, 263, 869
BstX2I RGATCY 2 cut(s) 84, 820
BstYI RGATCY 2 cut(s) 84, 820
BstZI CGGCCG 3 cut(s) 352, 356, 756
BsuRI GGCC 6 cut(s) 354, 358, 457, 680, 722, 758
BtgI CCRYGG 2 cut(s) 681, 759
BtrI CACGTC 1 cut(s) 640
BtsCI GGATG 2 cut(s) 282, 661
BtsI GCAGTG 2 cut(s) 306, 366
BtsIMutI CAGTG 3 cut(s) 32, 306, 366
Cfr10I RCCGGY 6 cut(s) 194, 227, 354, 358, 896, 986
Cfr13I GGNCC 3 cut(s) 678, 957, 1018
Cfr42I CCGCGG 1 cut(s) 762
CseI GACGC 1 cut(s) 608
CviAII CATG 6 cut(s) 43, 172, 562, 718, 742, 1015
DdeI CTNAG 3 cut(s) 74, 372, 887
DinI GGCGCC 2 cut(s) 226, 232
DpnI GATC 6 cut(s) 86, 510, 716, 822, 924, 1175
DpnII GATC 6 cut(s) 84, 508, 714, 820, 922, 1173
DrdI GACNNNNNNGTC 1 cut(s) 1237
DriI GACNNNNNGTC 1 cut(s) 565
DseDI GACNNNNNNGTC 1 cut(s) 1237
EaeI YGGCCR 5 cut(s) 352, 356, 455, 720, 756
EagI CGGCCG 3 cut(s) 352, 356, 756
Eam1104I CTCTTC 4 cut(s) 119, 168, 494, 1179
Eam1105I GACNNNNNGTC 1 cut(s) 565
EarI CTCTTC 4 cut(s) 119, 168, 494, 1179
EciI GGCGGA 3 cut(s) 275, 482, 747
EclXI CGGCCG 3 cut(s) 352, 356, 756
Eco47I GGWCC 2 cut(s) 957, 1018
Eco52I CGGCCG 3 cut(s) 352, 356, 756
Eco57I CTGAAG 1 cut(s) 894
Eco72I CACGTG 1 cut(s) 654
Eco88I CYCGRG 2 cut(s) 98, 272
EgeI GGCGCC 2 cut(s) 226, 232
EheI GGCGCC 2 cut(s) 226, 232
FaeI CATG 6 cut(s) 46, 175, 565, 721, 745, 1018
FaiI YATR 9 cut(s) 44, 173, 563, 719, 743, 969, 995, 1016, 1055
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FaqI GGGAC 1 cut(s) 553
FatI CATG 6 cut(s) 42, 171, 561, 717, 741, 1014
FauI CCCGC 1 cut(s) 578
FbaI TGATCA 2 cut(s) 922, 1173
FblI GTMKAC 4 cut(s) 60, 425, 699, 726
FokI GGATG 2 cut(s) 269, 648
FseI GGCCGGCC 1 cut(s) 358
FspBI CTAG 1 cut(s) 180
HaeII RGCGCY 2 cut(s) 228, 234
HaeIII GGCC 6 cut(s) 354, 358, 457, 680, 722, 758
HgaI GACGC 1 cut(s) 608
Hin1I GRCGYC 2 cut(s) 225, 231
Hin1II CATG 6 cut(s) 46, 175, 565, 721, 745, 1018
HincII GTYRAC 4 cut(s) 61, 67, 700, 727
HindII GTYRAC 4 cut(s) 61, 67, 700, 727
HinfI GANTC 9 cut(s) 47, 207, 275, 302, 547, 851, 891, 1097, 1276
HphI GGTGA 2 cut(s) 184, 1183
Hpy166II GTNNAC 8 cut(s) 61, 67, 268, 366, 426, 645, 700, 727
Hpy188I TCNGA 8 cut(s) 206, 322, 753, 780, 842, 856, 863, 913
Hpy188III TCNNGA 4 cut(s) 98, 100, 374, 628
Hpy8I GTNNAC 8 cut(s) 61, 67, 268, 366, 426, 645, 700, 727
HpyAV CCTTC 6 cut(s) 457, 466, 517, 628, 885, 896
HpyCH4III ACNGT 2 cut(s) 36, 1216
HpyCH4IV ACGT 6 cut(s) 63, 500, 639, 653, 774, 1092
HpyCH4V TGCA 4 cut(s) 282, 431, 645, 979
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 14, 288, 297, 351, 377, 656, 665, 677
HpyF3I CTNAG 3 cut(s) 74, 372, 887
HpySE526I ACGT 6 cut(s) 63, 500, 639, 653, 774, 1092
Hsp92I GRCGYC 2 cut(s) 225, 231
Hsp92II CATG 6 cut(s) 46, 175, 565, 721, 745, 1018
KasI GGCGCC 2 cut(s) 224, 230
KroI GCCGGC 3 cut(s) 227, 354, 896
KroNI GCCGGC 3 cut(s) 229, 356, 898
Ksp22I TGATCA 2 cut(s) 922, 1173
KspI CCGCGG 1 cut(s) 762
Kzo9I GATC 6 cut(s) 84, 508, 714, 820, 922, 1173
LguI GCTCTTC 1 cut(s) 494
LmnI GCTCC 2 cut(s) 350, 905
Lsp1109I GCAGC 5 cut(s) 20, 23, 26, 263, 869
LweI GCATC 3 cut(s) 291, 670, 999
MaeI CTAG 1 cut(s) 180
MaeII ACGT 6 cut(s) 63, 500, 639, 653, 774, 1092
MaeIII GTNAC 2 cut(s) 119, 834
MalI GATC 6 cut(s) 86, 510, 716, 822, 924, 1175
MboI GATC 6 cut(s) 84, 508, 714, 820, 922, 1173
MflI RGATCY 2 cut(s) 84, 820
MhlI GDGCHC 2 cut(s) 647, 1136
MluCI AATT 3 cut(s) 735, 964, 1138
Mly113I GGCGCC 2 cut(s) 225, 231
MlyI GAGTC 6 cut(s) 41, 284, 296, 860, 1106, 1285
MmeI TCCRAC 4 cut(s) 776, 834, 1050, 1131
MreI CGCCGGCG 2 cut(s) 227, 896
MroNI GCCGGC 3 cut(s) 227, 354, 896
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 4 cut(s) 212, 534, 690, 1064
MslI CAYNNNNRTG 1 cut(s) 1288
MspA1I CMGCKG 2 cut(s) 514, 761
MspCI CTTAAG 1 cut(s) 533
MspR9I CCNGG 2 cut(s) 443, 955
MvnI CGCG 8 cut(s) 573, 575, 592, 594, 659, 761, 975, 1012
MwoI GCNNNNNNNGC 9 cut(s) 11, 14, 288, 297, 351, 377, 656, 665, 677
NaeI GCCGGC 3 cut(s) 229, 356, 898
NarI GGCGCC 2 cut(s) 225, 231
NciI CCSGG 2 cut(s) 443, 955
NdeII GATC 6 cut(s) 84, 508, 714, 820, 922, 1173
NgoMIV GCCGGC 3 cut(s) 227, 354, 896
NlaIII CATG 6 cut(s) 46, 175, 565, 721, 745, 1018
NlaIV GGNNCC 4 cut(s) 226, 232, 346, 679
NmuCI GTSAC 1 cut(s) 834
NspI RCATGY 1 cut(s) 565
PaeR7I CTCGAG 2 cut(s) 98, 272
PauI GCGCGC 4 cut(s) 573, 592, 657, 1010
PciI ACATGT 1 cut(s) 561
PciSI GCTCTTC 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PdiI GCCGGC 3 cut(s) 229, 356, 898
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 3 cut(s) 207, 547, 891
PfoI TCCNGGA 1 cut(s) 953
PleI GAGTC 6 cut(s) 41, 283, 296, 859, 1105, 1284
PluTI GGCGCC 2 cut(s) 228, 234
PmaCI CACGTG 1 cut(s) 654
PmlI CACGTG 1 cut(s) 654
PpsI GAGTC 6 cut(s) 41, 283, 296, 859, 1105, 1284
Ppu21I YACGTR 1 cut(s) 654
PscI ACATGT 1 cut(s) 561
PspCI CACGTG 1 cut(s) 654
PspN4I GGNNCC 4 cut(s) 226, 232, 346, 679
PspPI GGNCC 3 cut(s) 678, 957, 1018
PspXI VCTCGAGB 1 cut(s) 272
PstI CTGCAG 1 cut(s) 433
PsuI RGATCY 2 cut(s) 84, 820
PteI GCGCGC 4 cut(s) 573, 592, 657, 1010
PvuII CAGCTG 1 cut(s) 514
RigI GGCCGGCC 1 cut(s) 358
RseI CAYNNNNRTG 1 cut(s) 1288
SacII CCGCGG 1 cut(s) 762
SalI GTCGAC 3 cut(s) 59, 698, 725
SapI GCTCTTC 1 cut(s) 494
SaqAI TTAA 4 cut(s) 212, 534, 690, 1064
Sau3AI GATC 6 cut(s) 84, 508, 714, 820, 922, 1173
Sau96I GGNCC 3 cut(s) 678, 957, 1018
SchI GAGTC 6 cut(s) 41, 284, 296, 860, 1106, 1285
ScrFI CCNGG 2 cut(s) 443, 955
SduI GDGCHC 2 cut(s) 647, 1136
SfaNI GCATC 3 cut(s) 291, 670, 999
SfcI CTRYAG 1 cut(s) 429
SfoI GGCGCC 2 cut(s) 226, 232
Sfr274I CTCGAG 2 cut(s) 98, 272
Sfr303I CCGCGG 1 cut(s) 762
SgrAI CRCCGGYG 2 cut(s) 227, 896
SgrBI CCGCGG 1 cut(s) 762
SgrDI CGTCGACG 2 cut(s) 59, 725
SinI GGWCC 2 cut(s) 957, 1018
SlaI CTCGAG 2 cut(s) 98, 272
SmiMI CAYNNNNRTG 1 cut(s) 1288
SmlI CTYRAG 3 cut(s) 98, 272, 533
SmoI CTYRAG 3 cut(s) 98, 272, 533
Sse9I AATT 3 cut(s) 735, 964, 1138
SspDI GGCGCC 2 cut(s) 224, 230
SspI AATATT 1 cut(s) 438
SspMI CTAG 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 441, 953
TaaI ACNGT 2 cut(s) 36, 1216
TaiI ACGT 6 cut(s) 66, 503, 642, 656, 777, 1095
TasI AATT 3 cut(s) 735, 964, 1138
TauI GCSGC 7 cut(s) 8, 291, 314, 354, 671, 761, 764
TfiI GAWTC 3 cut(s) 207, 547, 891
Tru1I TTAA 4 cut(s) 212, 534, 690, 1064
Tru9I TTAA 4 cut(s) 212, 534, 690, 1064
TscAI CASTG 3 cut(s) 39, 313, 373
TseFI GTSAC 1 cut(s) 834
TseI GCWGC 5 cut(s) 8, 11, 14, 251, 882
Tsp45I GTSAC 1 cut(s) 834
TspDTI ATGAA 5 cut(s) 155, 188, 536, 647, 758
TspGWI ACGGA 2 cut(s) 46, 789
TspRI CASTG 3 cut(s) 39, 313, 373
Vha464I CTTAAG 1 cut(s) 533
VneI GTGCAC 1 cut(s) 643
VpaK11BI GGWCC 2 cut(s) 957, 1018
XapI RAATTY 1 cut(s) 1138
XceI RCATGY 1 cut(s) 565
XhoI CTCGAG 2 cut(s) 98, 272
XmiI GTMKAC 4 cut(s) 60, 425, 699, 726
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.