FvH4_3g11670

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009521
Physical Location & Seq
Reverse (-)
6888054 .. 6889811
1758 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11670.t2

Sequence Viewer

Length: 501 bp
ATGGCAACAGAAATGAAGGACACGAAGGGAGGCGAGGTGCAAATAGATATGGAGGCGGCGAGGAGATACTTGGAGAAGGAAGGTGAAACCGCAGCCTCCATGGACTCGCTGCCTGATAAGTTCTTTGAGCCTTTTATCATGAAAGGCATTAAAGTTGATCGCATTGAACGCGGCACAATTGTCTGCTCCTTCAAAGTACCCCCACGGTTGCTGAATGGGGGCGGTTTCCTGCACGGTGGAGCTACGGCAACCCTTGTGGATTTGGTTGGGTCAGCTGTAATCTATACTGTTGGAGCTCCCACTGTTGGAGTGTCTGTGGAGATCAATATTTCGTACTTGGATTCTGCTTATGCTGGGGAGGATGTTGAGATTGAGGCCAAGGCTTTGAGGGTTGGGAAAGCAGTGGCTGTTGTTAGTATTGAGTTCAGGAAGAAGAAGACGGGGAAGATTATTGCTCAGGGGCGCCATACCAAGTACCTTGCCCTCGCTAGCAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

167

Amino Acids

17.84

Weight (kDa)

8.59

Isoelectric Point (pI)

25.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 74 - 149 7.8e-18 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 462
AccII CGCG 1 cut(s) 171
AciI CCGC 4 cut(s) 56, 90, 171, 222
AcyI GRCGYC 1 cut(s) 463
AfaI GTAC 3 cut(s) 198, 335, 476
AfiI CCNNNNNNNGG 1 cut(s) 305
AgsI TTSAA 2 cut(s) 167, 193
AluBI AGCT 3 cut(s) 242, 275, 296
AluI AGCT 3 cut(s) 242, 275, 296
Alw21I GWGCWC 1 cut(s) 298
AlwNI CAGNNNCTG 1 cut(s) 407
AoxI GGCC 1 cut(s) 375
ApeKI GCWGC 2 cut(s) 92, 109
AspLEI GCGC 1 cut(s) 465
AsuHPI GGTGA 1 cut(s) 95
AsuNHI GCTAGC 1 cut(s) 488
BanI GGYRCC 1 cut(s) 462
BanII GRGCYC 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 298
BbvI GCAGC 2 cut(s) 96, 104
BceAI ACGGC 1 cut(s) 261
BfaI CTAG 1 cut(s) 489
BfoI RGCGCY 1 cut(s) 466
BisI GCNGC 4 cut(s) 57, 93, 110, 172
BlsI GCNGC 4 cut(s) 58, 94, 111, 173
BmiI GGNNCC 1 cut(s) 464
BmtI GCTAGC 1 cut(s) 492
BpiI GAAGAC 1 cut(s) 443
Bpu10I CCTNAGC 1 cut(s) 456
BsaHI GRCGYC 1 cut(s) 463
BsaJI CCNNGG 3 cut(s) 99, 203, 378
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 300, 330
Bsc4I CCNNNNNNNGG 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 99, 203, 378
BseGI GGATG 1 cut(s) 367
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMII CTCAG 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 76
BseXI GCAGC 2 cut(s) 96, 104
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 1 cut(s) 377
BshNI GGYRCC 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 305
BsnI GGCC 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 2 cut(s) 157, 321
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 4 cut(s) 56, 90, 171, 222
BspANI GGCC 1 cut(s) 377
BspCNI CTCAG 1 cut(s) 469
BspFNI CGCG 1 cut(s) 171
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 464
BspOI GCTAGC 1 cut(s) 492
BspT107I GGYRCC 1 cut(s) 462
BssECI CCNNGG 3 cut(s) 99, 203, 378
BssMI GATC 2 cut(s) 157, 321
BssNI GRCGYC 1 cut(s) 463
BssT1I CCWWGG 2 cut(s) 99, 378
Bst4CI ACNGT 4 cut(s) 207, 236, 289, 304
BstACI GRCGYC 1 cut(s) 463
BstC8I GCNNGC 1 cut(s) 490
BstDEI CTNAG 1 cut(s) 456
BstDSI CCRYGG 2 cut(s) 99, 203
BstF5I GGATG 1 cut(s) 367
BstFNI CGCG 1 cut(s) 171
BstH2I RGCGCY 1 cut(s) 466
BstHHI GCGC 1 cut(s) 465
BstKTI GATC 2 cut(s) 160, 324
BstMBI GATC 2 cut(s) 157, 321
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 96, 104
BstV2I GAAGAC 1 cut(s) 443
BsuRI GGCC 1 cut(s) 377
BtgI CCRYGG 2 cut(s) 99, 203
BtsCI GGATG 1 cut(s) 367
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 2 cut(s) 300, 408
Cac8I GCNNGC 1 cut(s) 490
CaiI CAGNNNCTG 1 cut(s) 407
CciI TCATGA 1 cut(s) 138
CfoI GCGC 1 cut(s) 465
Csp6I GTAC 3 cut(s) 197, 334, 475
CspCI CAANNNNNGTGG 2 cut(s) 237, 272
CviAII CATG 2 cut(s) 100, 139
CviJI RGCY 8 cut(s) 95, 130, 242, 275, 296, 377, 383, 407
CviKI_1 RGCY 8 cut(s) 95, 130, 242, 275, 296, 377, 383, 407
CviQI GTAC 3 cut(s) 197, 334, 475
DdeI CTNAG 1 cut(s) 456
DinI GGCGCC 1 cut(s) 464
DpnI GATC 2 cut(s) 159, 323
DpnII GATC 2 cut(s) 157, 321
Ecl136II GAGCTC 1 cut(s) 296
Eco130I CCWWGG 2 cut(s) 99, 378
Eco24I GRGCYC 1 cut(s) 298
Eco53kI GAGCTC 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 296
EcoT14I CCWWGG 2 cut(s) 99, 378
EcoT38I GRGCYC 1 cut(s) 298
EgeI GGCGCC 1 cut(s) 464
EheI GGCGCC 1 cut(s) 464
ErhI CCWWGG 2 cut(s) 99, 378
FaeI CATG 2 cut(s) 103, 142
FaiI YATR 6 cut(s) 50, 101, 140, 285, 351, 468
FatI CATG 2 cut(s) 99, 138
Fnu4HI GCNGC 4 cut(s) 57, 93, 110, 172
FokI GGATG 1 cut(s) 374
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 4 cut(s) 57, 93, 110, 172
FspBI CTAG 1 cut(s) 489
GlaI GCGC 1 cut(s) 464
GluI GCNGC 4 cut(s) 57, 93, 110, 172
GsaI CCCAGC 1 cut(s) 357
HaeII RGCGCY 1 cut(s) 466
HaeIII GGCC 1 cut(s) 377
HhaI GCGC 1 cut(s) 465
Hin1I GRCGYC 1 cut(s) 463
Hin1II CATG 2 cut(s) 103, 142
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HinfI GANTC 2 cut(s) 104, 341
HphI GGTGA 1 cut(s) 95
Hpy188III TCNNGA 2 cut(s) 139, 427
HpyAV CCTTC 5 cut(s) 10, 19, 70, 74, 199
HpyCH4III ACNGT 4 cut(s) 207, 236, 289, 304
HpyCH4V TGCA 2 cut(s) 40, 232
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpyF3I CTNAG 1 cut(s) 456
Hsp92I GRCGYC 1 cut(s) 463
Hsp92II CATG 2 cut(s) 103, 142
HspAI GCGC 1 cut(s) 463
KasI GGCGCC 1 cut(s) 462
Kzo9I GATC 2 cut(s) 157, 321
LmnI GCTCC 4 cut(s) 191, 239, 293, 301
LpnPI CCDG 5 cut(s) 126, 242, 339, 412, 443
Lsp1109I GCAGC 2 cut(s) 96, 104
MaeI CTAG 1 cut(s) 489
MalI GATC 2 cut(s) 159, 323
MboI GATC 2 cut(s) 157, 321
MboII GAAGA 4 cut(s) 442, 445, 448, 457
MfeI CAATTG 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 298
MluCI AATT 2 cut(s) 177, 494
Mly113I GGCGCC 1 cut(s) 463
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 2 cut(s) 271, 286
MnlI CCTC 9 cut(s) 23, 28, 46, 54, 106, 352, 367, 381, 494
MseI TTAA 1 cut(s) 150
MspA1I CMGCKG 1 cut(s) 275
MunI CAATTG 1 cut(s) 177
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 1 cut(s) 168
NarI GGCGCC 1 cut(s) 463
NcoI CCATGG 1 cut(s) 99
NdeII GATC 2 cut(s) 157, 321
NheI GCTAGC 1 cut(s) 488
NlaIII CATG 2 cut(s) 103, 142
NlaIV GGNNCC 1 cut(s) 464
PagI TCATGA 1 cut(s) 138
PfeI GAWTC 1 cut(s) 341
PkrI GCNGC 4 cut(s) 58, 94, 111, 173
PleI GAGTC 1 cut(s) 98
PluTI GGCGCC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 353
PspN4I GGNNCC 1 cut(s) 464
PstNI CAGNNNCTG 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 3 cut(s) 198, 335, 476
RsaNI GTAC 3 cut(s) 197, 334, 475
SacI GAGCTC 1 cut(s) 298
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 4 cut(s) 57, 93, 110, 172
Sau3AI GATC 2 cut(s) 157, 321
SchI GAGTC 1 cut(s) 98
SduI GDGCHC 1 cut(s) 298
SetI ASST 6 cut(s) 39, 85, 244, 277, 298, 480
SfoI GGCGCC 1 cut(s) 464
Sse9I AATT 2 cut(s) 177, 494
SsiI CCGC 4 cut(s) 56, 90, 171, 222
SspDI GGCGCC 1 cut(s) 462
SspI AATATT 1 cut(s) 328
SspMI CTAG 1 cut(s) 489
SstI GAGCTC 1 cut(s) 298
StyI CCWWGG 2 cut(s) 99, 378
TaaI ACNGT 4 cut(s) 207, 236, 289, 304
TasI AATT 2 cut(s) 177, 494
TauI GCSGC 2 cut(s) 59, 174
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TscAI CASTG 2 cut(s) 307, 408
TseI GCWGC 2 cut(s) 92, 109
TspDTI ATGAA 2 cut(s) 29, 155
TspRI CASTG 2 cut(s) 307, 408
XspI CTAG 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.