MD05G1260500.v1.1

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009521
Physical Location & Seq
Forward (+)
39570773 .. 39572376
1604 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1260500.v1.1.491

Sequence Viewer

Length: 483 bp
ATGAAGAAAGAAGGAGCCGAGGAAACAAGCATGGAGATAGTGAAGAAGTTTTTGGAGAAAGAAGGTGAAATGGCAATCGCCATGGATAGTCTGCCCGACAGGTTCTTTGAGCCCTTCATCCTGAAAGGCATTAACGTCGATCTCCTTGAACCCGGACACATTGTTTGCTCCTTCAAAGTACCCCCGCGTTTGGTGAACGGGGGCAATTTCTTGCACGGTGGAGCCACAGCAACCCTTGTGGATTTGATAGGATCAGCTGCAATCCTCTCCTCTGGAGCTCTCAGTTCCGGAGTTTCGGTGGAGATCAATGTGTCGTACTTGGATTCGGCTTATCCTGGCGAGGAAGTTGAGGTCGAGTCCAGGACGTTACGTGTTGGGAAGACTGTTGGTGTTGCCAGTGTTGAGATCAGGAAGAAGAAGACTGGGAAGATTATTGCTCAGGGCCGTCATACCAAGTACCTTGCTGTTTCTAGCAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

161

Amino Acids

17.15

Weight (kDa)

6.84

Isoelectric Point (pI)

26.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 69 - 143 1.2e-16 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 187
AccIII TCCGGA 1 cut(s) 287
AciI CCGC 1 cut(s) 185
AclWI GGATC 1 cut(s) 259
AfaI GTAC 3 cut(s) 180, 317, 458
AfiI CCNNNNNNNGG 1 cut(s) 190
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 2 cut(s) 149, 175
AjnI CCWGG 2 cut(s) 334, 359
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
AluBI AGCT 2 cut(s) 257, 278
AluI AGCT 2 cut(s) 257, 278
Alw21I GWGCWC 1 cut(s) 280
AlwI GGATC 1 cut(s) 259
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 1 cut(s) 442
ApeKI GCWGC 1 cut(s) 257
ArsI GACNNNNNNTTYG 4 cut(s) 89, 121, 147, 179
AspS9I GGNCC 1 cut(s) 442
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 2 cut(s) 77, 205
BanII GRGCYC 2 cut(s) 114, 280
BbsI GAAGAC 2 cut(s) 386, 425
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 1 cut(s) 244
BceAI ACGGC 1 cut(s) 429
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 2 cut(s) 336, 361
BcnI CCSGG 1 cut(s) 153
BfaI CTAG 1 cut(s) 471
BisI GCNGC 1 cut(s) 258
BlsI GCNGC 1 cut(s) 259
Bme1390I CCNGG 3 cut(s) 153, 336, 361
BmgT120I GGNCC 1 cut(s) 442
BmiI GGNNCC 2 cut(s) 16, 223
BmrFI CCNGG 3 cut(s) 153, 336, 361
BmrI ACTGGG 1 cut(s) 432
BmuI ACTGGG 1 cut(s) 432
BpiI GAAGAC 2 cut(s) 386, 425
BpmI CTGGAG 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 153
BsaAI YACGTR 1 cut(s) 371
BsaJI CCNNGG 2 cut(s) 18, 81
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse1I ACTGG 2 cut(s) 396, 427
BseAI TCCGGA 1 cut(s) 287
BseBI CCWGG 2 cut(s) 336, 361
BseDI CCNNGG 2 cut(s) 18, 81
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 2 cut(s) 295, 452
BseNI ACTGG 2 cut(s) 396, 427
BseRI GAGGAG 1 cut(s) 259
BseXI GCAGC 1 cut(s) 244
Bsh1236I CGCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 444
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 2 cut(s) 153, 288
BslI CCNNNNNNNGG 1 cut(s) 190
BsnI GGCC 1 cut(s) 444
Bsp1286I GDGCHC 2 cut(s) 114, 280
Bsp13I TCCGGA 1 cut(s) 287
Bsp143I GATC 4 cut(s) 139, 251, 303, 405
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 1 cut(s) 185
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 2 cut(s) 294, 451
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 1 cut(s) 187
BspLI GGNNCC 2 cut(s) 16, 223
BspPI GGATC 1 cut(s) 259
BsrI ACTGG 2 cut(s) 396, 427
BssECI CCNNGG 2 cut(s) 18, 81
BssMI GATC 4 cut(s) 139, 251, 303, 405
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 2 cut(s) 336, 361
Bst4CI ACNGT 2 cut(s) 218, 385
BstBAI YACGTR 1 cut(s) 371
BstDEI CTNAG 2 cut(s) 281, 438
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 1 cut(s) 117
BstFNI CGCG 1 cut(s) 187
BstKTI GATC 4 cut(s) 142, 254, 306, 408
BstMBI GATC 4 cut(s) 139, 251, 303, 405
BstNI CCWGG 2 cut(s) 336, 361
BstSCI CCNGG 3 cut(s) 151, 334, 359
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 244
BstV2I GAAGAC 2 cut(s) 386, 425
BsuRI GGCC 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 403
Cfr13I GGNCC 1 cut(s) 442
Csp6I GTAC 3 cut(s) 179, 316, 457
CspCI CAANNNNNGTGG 2 cut(s) 219, 254
CviAII CATG 2 cut(s) 31, 82
CviJI RGCY 7 cut(s) 17, 112, 224, 257, 278, 329, 444
CviKI_1 RGCY 7 cut(s) 17, 112, 224, 257, 278, 329, 444
CviQI GTAC 3 cut(s) 179, 316, 457
DdeI CTNAG 2 cut(s) 281, 438
DpnI GATC 4 cut(s) 141, 253, 305, 407
DpnII GATC 4 cut(s) 139, 251, 303, 405
Ecl136II GAGCTC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 2 cut(s) 114, 280
Eco53kI GAGCTC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 334, 359
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 2 cut(s) 114, 280
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 2 cut(s) 34, 85
FaiI YATR 3 cut(s) 32, 83, 450
FatI CATG 2 cut(s) 30, 81
FauI CCCGC 1 cut(s) 192
Fnu4HI GCNGC 1 cut(s) 258
FokI GGATG 1 cut(s) 104
FriOI GRGCYC 2 cut(s) 114, 280
Fsp4HI GCNGC 1 cut(s) 258
FspBI CTAG 1 cut(s) 471
GluI GCNGC 1 cut(s) 258
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 1 cut(s) 444
HapII CCGG 2 cut(s) 153, 288
Hin1II CATG 2 cut(s) 34, 85
HinfI GANTC 2 cut(s) 323, 356
HpaII CCGG 2 cut(s) 153, 288
HphI GGTGA 2 cut(s) 77, 205
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 1 cut(s) 482
Hpy188III TCNNGA 4 cut(s) 121, 273, 288, 409
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 4 cut(s) 5, 56, 124, 181
HpyCH4III ACNGT 2 cut(s) 218, 385
HpyCH4IV ACGT 3 cut(s) 135, 365, 370
HpyCH4V TGCA 2 cut(s) 214, 260
HpyF3I CTNAG 2 cut(s) 281, 438
HpySE526I ACGT 3 cut(s) 135, 365, 370
Hsp92II CATG 2 cut(s) 34, 85
Kpn2I TCCGGA 1 cut(s) 287
Kzo9I GATC 4 cut(s) 139, 251, 303, 405
LmnI GCTCC 4 cut(s) 14, 173, 221, 275
Lsp1109I GCAGC 1 cut(s) 244
MaeI CTAG 1 cut(s) 471
MaeII ACGT 3 cut(s) 135, 365, 370
MaeIII GTNAC 1 cut(s) 366
MalI GATC 4 cut(s) 141, 253, 305, 407
MboI GATC 4 cut(s) 139, 251, 303, 405
MboII GAAGA 7 cut(s) 16, 55, 391, 424, 427, 430, 439
MhlI GDGCHC 2 cut(s) 114, 280
MluCI AATT 1 cut(s) 205
MlyI GAGTC 1 cut(s) 365
MnlI CCTC 5 cut(s) 13, 275, 280, 334, 343
MroI TCCGGA 1 cut(s) 287
MseI TTAA 1 cut(s) 132
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 2 cut(s) 153, 288
MspR9I CCNGG 3 cut(s) 153, 336, 361
MvaI CCWGG 2 cut(s) 336, 361
MvnI CGCG 1 cut(s) 187
NciI CCSGG 1 cut(s) 153
NcoI CCATGG 1 cut(s) 81
NdeII GATC 4 cut(s) 139, 251, 303, 405
NlaIII CATG 2 cut(s) 34, 85
NlaIV GGNNCC 2 cut(s) 16, 223
NmeAIII GCCGAG 1 cut(s) 43
PfeI GAWTC 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 359
PkrI GCNGC 1 cut(s) 259
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
Ppu21I YACGTR 1 cut(s) 371
Psp124BI GAGCTC 1 cut(s) 280
Psp6I CCWGG 2 cut(s) 334, 359
PspGI CCWGG 2 cut(s) 334, 359
PspN4I GGNNCC 2 cut(s) 16, 223
PspPI GGNCC 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 3 cut(s) 180, 317, 458
RsaNI GTAC 3 cut(s) 179, 316, 457
SacI GAGCTC 1 cut(s) 280
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 4 cut(s) 139, 251, 303, 405
Sau96I GGNCC 1 cut(s) 442
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 3 cut(s) 153, 336, 361
SduI GDGCHC 2 cut(s) 114, 280
SetI ASST 9 cut(s) 67, 104, 138, 259, 280, 354, 368, 373, 462
Sse9I AATT 1 cut(s) 205
SsiI CCGC 1 cut(s) 185
SspMI CTAG 1 cut(s) 471
SstI GAGCTC 1 cut(s) 280
StyD4I CCNGG 3 cut(s) 151, 334, 359
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 2 cut(s) 218, 385
TaiI ACGT 3 cut(s) 138, 368, 373
TaqI TCGA 2 cut(s) 138, 354
TasI AATT 1 cut(s) 205
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TscAI CASTG 1 cut(s) 403
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 2 cut(s) 17, 106
TspRI CASTG 1 cut(s) 403
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.