MD10G1239800.v1.1

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009521
Physical Location & Seq
Forward (+)
33547193 .. 33548339
1147 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1239800.v1.1.491

Sequence Viewer

Length: 483 bp
ATGAAGAAAGAAGGAACCGAGGAAACAAGCATGGAGATGGTGAAGTTGTTTTTGGAGAAAAAAGGTGAAATGGCAGTCGCCATGGATACTCTGCCGGACAAGTTCTTCGAGCCCTTTATCATGAAGGGCGTCAAAATCGACCTCCTTGAACCCGGACGCATTGTTTGCTCCTTCAAGGTGCCCCCGCGTCTGCTGAACGGGGGCAATTTCATGCACGGCGGAGCCACAGCAACCCTTGTGGATTTGGTAGGATCAGCTGCACTCCTCTCCTCCGGAGCTCTCAACGTTGGAGTTTCGGTGGAGATCAATGTGTCGTACTTGGATTCGGCTTATCCCGGCGAGGATGTTGAGATCGAGTCCAGGACGTTACGTGTTGGAAAAAGTGTTGGTGTTGTGAGTGTTGAGTTCAGGAAGAAGAAGACTGGGAAGATTATCGCGCAGGGCCGTCATACCAAGTACCTCGCTGTTTCTAGCAAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

161

Amino Acids

17.31

Weight (kDa)

8.95

Isoelectric Point (pI)

23.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 69 - 143 7e-17 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 178
AccII CGCG 2 cut(s) 187, 437
AccIII TCCGGA 1 cut(s) 272
AciI CCGC 2 cut(s) 185, 219
AclI AACGTT 1 cut(s) 285
AclWI GGATC 1 cut(s) 259
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 2 cut(s) 317, 458
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 2 cut(s) 149, 175
AjnI CCWGG 1 cut(s) 359
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
AluBI AGCT 2 cut(s) 257, 278
AluI AGCT 2 cut(s) 257, 278
Alw21I GWGCWC 1 cut(s) 280
AlwI GGATC 1 cut(s) 259
Aor13HI TCCGGA 1 cut(s) 272
AoxI GGCC 1 cut(s) 442
ApeKI GCWGC 1 cut(s) 257
ArsI GACNNNNNNTTYG 4 cut(s) 89, 121, 147, 179
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 1 cut(s) 442
AsuC2I CCSGG 2 cut(s) 153, 336
AsuHPI GGTGA 2 cut(s) 52, 77
BaeGI GKGCMC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 178
BanII GRGCYC 2 cut(s) 114, 280
BbsI GAAGAC 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 1 cut(s) 244
BccI CCATC 1 cut(s) 31
BceAI ACGGC 2 cut(s) 232, 429
BciT130I CCWGG 1 cut(s) 361
BciVI GTATCC 1 cut(s) 79
BcnI CCSGG 2 cut(s) 153, 336
BfaI CTAG 1 cut(s) 471
BfuI GTATCC 1 cut(s) 79
BisI GCNGC 1 cut(s) 258
BlsI GCNGC 1 cut(s) 259
Bme1390I CCNGG 3 cut(s) 153, 336, 361
BmgT120I GGNCC 1 cut(s) 442
BmiI GGNNCC 3 cut(s) 16, 180, 223
BmrFI CCNGG 3 cut(s) 153, 336, 361
BmrI ACTGGG 1 cut(s) 432
BmuI ACTGGG 1 cut(s) 432
BpiI GAAGAC 1 cut(s) 425
BpuMI CCSGG 2 cut(s) 153, 336
BsaAI YACGTR 1 cut(s) 371
BsaHI GRCGYC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 18, 81
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bse1I ACTGG 1 cut(s) 427
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 1 cut(s) 361
BseDI CCNNGG 2 cut(s) 18, 81
BseGI GGATG 1 cut(s) 349
BseNI ACTGG 1 cut(s) 427
BseRI GAGGAG 2 cut(s) 254, 259
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 1 cut(s) 244
BsgI GTGCAG 1 cut(s) 243
Bsh1236I CGCG 2 cut(s) 187, 437
BshFI GGCC 1 cut(s) 444
BshNI GGYRCC 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 4 cut(s) 95, 153, 273, 336
BsnI GGCC 1 cut(s) 444
Bsp1286I GDGCHC 3 cut(s) 114, 183, 280
Bsp13I TCCGGA 1 cut(s) 272
Bsp143I GATC 3 cut(s) 251, 303, 351
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 2 cut(s) 185, 219
BspANI GGCC 1 cut(s) 444
BspEI TCCGGA 1 cut(s) 272
BspFNI CGCG 2 cut(s) 187, 437
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 3 cut(s) 16, 180, 223
BspPI GGATC 1 cut(s) 259
BspT107I GGYRCC 1 cut(s) 178
BsrI ACTGG 1 cut(s) 427
BssECI CCNNGG 2 cut(s) 18, 81
BssMI GATC 3 cut(s) 251, 303, 351
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 361
Bst4CI ACNGT 1 cut(s) 480
BstACI GRCGYC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 165
BstBAI YACGTR 1 cut(s) 371
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 2 cut(s) 187, 437
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 3 cut(s) 254, 306, 354
BstMBI GATC 3 cut(s) 251, 303, 351
BstMWI GCNNNNNNNGC 1 cut(s) 165
BstNI CCWGG 1 cut(s) 361
BstSCI CCNGG 3 cut(s) 151, 334, 359
BstSLI GKGCMC 1 cut(s) 183
BstUI CGCG 2 cut(s) 187, 437
BstV1I GCAGC 1 cut(s) 244
BstV2I GAAGAC 1 cut(s) 425
BsuI GTATCC 1 cut(s) 79
BsuRI GGCC 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 349
CciI TCATGA 1 cut(s) 120
CfoI GCGC 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 442
CseI GACGC 3 cut(s) 118, 165, 176
Csp6I GTAC 2 cut(s) 316, 457
CspCI CAANNNNNGTGG 2 cut(s) 219, 254
CviAII CATG 4 cut(s) 31, 82, 121, 211
CviJI RGCY 6 cut(s) 112, 224, 257, 278, 329, 444
CviKI_1 RGCY 6 cut(s) 112, 224, 257, 278, 329, 444
CviQI GTAC 2 cut(s) 316, 457
DpnI GATC 3 cut(s) 253, 305, 353
DpnII GATC 3 cut(s) 251, 303, 351
EciI GGCGGA 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 2 cut(s) 114, 280
Eco53kI GAGCTC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 359
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 2 cut(s) 114, 280
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 4 cut(s) 34, 85, 124, 214
FaiI YATR 5 cut(s) 32, 83, 122, 212, 450
FatI CATG 4 cut(s) 30, 81, 120, 210
FauI CCCGC 1 cut(s) 192
Fnu4HI GCNGC 1 cut(s) 258
FokI GGATG 1 cut(s) 356
FriOI GRGCYC 2 cut(s) 114, 280
Fsp4HI GCNGC 1 cut(s) 258
FspBI CTAG 1 cut(s) 471
GlaI GCGC 1 cut(s) 438
GluI GCNGC 1 cut(s) 258
HaeIII GGCC 1 cut(s) 444
HapII CCGG 4 cut(s) 95, 153, 273, 336
HgaI GACGC 3 cut(s) 118, 165, 176
HhaI GCGC 1 cut(s) 439
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 4 cut(s) 34, 85, 124, 214
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HinfI GANTC 2 cut(s) 323, 356
HpaII CCGG 4 cut(s) 95, 153, 273, 336
HphI GGTGA 2 cut(s) 52, 77
Hpy188III TCNNGA 3 cut(s) 121, 273, 409
HpyAV CCTTC 3 cut(s) 5, 118, 181
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 3 cut(s) 285, 365, 370
HpyCH4V TGCA 2 cut(s) 214, 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 165
HpySE526I ACGT 3 cut(s) 285, 365, 370
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 4 cut(s) 34, 85, 124, 214
HspAI GCGC 1 cut(s) 437
Kpn2I TCCGGA 1 cut(s) 272
Kzo9I GATC 3 cut(s) 251, 303, 351
LmnI GCTCC 3 cut(s) 173, 221, 275
LpnPI CCDG 9 cut(s) 108, 166, 286, 346, 349, 373, 394, 408, 425
Lsp1109I GCAGC 1 cut(s) 244
MaeI CTAG 1 cut(s) 471
MaeII ACGT 3 cut(s) 285, 365, 370
MaeIII GTNAC 1 cut(s) 366
MalI GATC 3 cut(s) 253, 305, 353
MboI GATC 3 cut(s) 251, 303, 351
MboII GAAGA 6 cut(s) 16, 97, 424, 427, 430, 439
MhlI GDGCHC 3 cut(s) 114, 183, 280
MluCI AATT 1 cut(s) 205
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 2 cut(s) 268, 355
MnlI CCTC 6 cut(s) 13, 152, 275, 280, 334, 470
MroI TCCGGA 1 cut(s) 272
MslI CAYNNNNRTG 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 4 cut(s) 95, 153, 273, 336
MspR9I CCNGG 3 cut(s) 153, 336, 361
MvaI CCWGG 1 cut(s) 361
MvnI CGCG 2 cut(s) 187, 437
MwoI GCNNNNNNNGC 1 cut(s) 165
NciI CCSGG 2 cut(s) 153, 336
NcoI CCATGG 1 cut(s) 81
NdeII GATC 3 cut(s) 251, 303, 351
NlaIII CATG 4 cut(s) 34, 85, 124, 214
NlaIV GGNNCC 3 cut(s) 16, 180, 223
PagI TCATGA 1 cut(s) 120
PfeI GAWTC 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 359
PkrI GCNGC 1 cut(s) 259
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
Ppu21I YACGTR 1 cut(s) 371
Psp124BI GAGCTC 1 cut(s) 280
Psp1406I AACGTT 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 359
PspGI CCWGG 1 cut(s) 359
PspN4I GGNNCC 3 cut(s) 16, 180, 223
PspPI GGNCC 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 317, 458
RsaNI GTAC 2 cut(s) 316, 457
RseI CAYNNNNRTG 1 cut(s) 35
SacI GAGCTC 1 cut(s) 280
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 3 cut(s) 251, 303, 351
Sau96I GGNCC 1 cut(s) 442
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 3 cut(s) 153, 336, 361
SduI GDGCHC 3 cut(s) 114, 183, 280
SetI ASST 9 cut(s) 67, 144, 180, 259, 280, 288, 368, 373, 462
SmiMI CAYNNNNRTG 1 cut(s) 35
Sse9I AATT 1 cut(s) 205
SsiI CCGC 2 cut(s) 185, 219
SspMI CTAG 1 cut(s) 471
SstI GAGCTC 1 cut(s) 280
StyD4I CCNGG 3 cut(s) 151, 334, 359
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 3 cut(s) 288, 368, 373
TaqI TCGA 3 cut(s) 108, 138, 354
TasI AATT 1 cut(s) 205
TfiI GAWTC 1 cut(s) 323
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 3 cut(s) 17, 137, 199
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.