Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0016134

Physical Location & Seq

Genomic Coordinates Forward (+)

8212861 .. 8215754

Gene Structure 2894 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g13661.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2307 bp

ATGAAGCCCCTGCTCCGCCCCATCTACTACTTCATCCCCAAACCCCCATGGCGGCGCCGCTATTCCCACACCCAACCAGACCCATCTCCCACCGTCAACGACCTCCTCACCCTCCTCGAAAAATCCGACGGCCACAACCAACTCATCTCTCTGATCCAGCAGCACCACGCCAACCCACAACTGGCCTTTCGTGTCTTCATCTGGGCCACCCAAAGATCCAAGGTTTGCACCAGAACCTGTCACTCTGCAATCGTCGACATGCTCGTTAAGGACGACAAACGGTTCGACATTTACTGGAGCACAATGCAGGAGCTCAGGGACTGCGGGGTTGGGATCGGGTGCGGCGCGTTTTCGGTGCTGATCAGAGGGTATGAGAGGCTGGGGAATGCCGAGAAGGCGGTGGAGGCTTTCGTAAAGATGGAGGAGTTTGATTGTAAGCCGGATGTTTATACTTACAATGCGGTTTTATATGTTATGGTGAGGAAAGAGGTGTTTTTGTTAGCTTTAGCTGTGTATAATCAGATGTTGAAGTGTAATCTTAGTCCTACTAGGAGTACTTATAGCATTTTGATCAATGGGTTTTGCAAGACTAGGAAAACCCAGGATGCATTGCAGATGTTTGATGAGATGGCACAGAGAGGGATTGCCCCCGATACGGTTACTTATACTATAATTGTTTCGGGGCTGTGCCAGGCGAAGAGGGCACACGAGGCGCATAGGCTGGTGGATAAGATGAGGGAGACTGGGTGTGTTCCGAATATTGTTACTTACCATGCTTTGCTTGATGGGTATTGTAAGTTAGGTAGGCTTGATGAGGCTTACGCGCTTGTGAGGTCGTTTCAGAGGATTGGTTATGTTCTTGGAGTAGAGGGATATAGTTCCTTGATTTTTGGGTTGTTTAGAGCTAGGAGATTTGATGAGGCGCTTGGGTTGTATGGCAAATTGCTTGGCGAGGGGATTGAGCCTGACGTTATCTTGTGTACTATATTGATTAAGGGGCTATCAGACGCTGGCAGGGTTAAGGATGCTTTGAATTTTTTGGGTGAGATGAGTAAGAAAGGTTTGGTTCCAGATGCCTACTGTTATAATGCTGTCATCAAGGGGTTCTGTGATTTAGGTCTTTTGGATGAAGCTCGATCTCTCCATCTTGAGATTTCGAAGCAAGACTGCTTCCCTAATGCCTGCACTTACACCATTCTCATTTGTGGTATGTGCAGGAACGGGCTGGTAGGGGAGGCAGAACAGATATTCAATGAGATGGAGAAGCTTGGATGTGTTCCATGTGTTGTAACCTTCAATGCTCTTATTGATGGGCTTTGCAAGGCTTCTAAGCTTAAGGATGCACACATGCTGTTTTACAAGATGGAGATAGGAAGAAAGCCTTCATTATTTCTTCGTCTTTCTCAAGGCAGTGATCGAATTATTGATAGTGCGAGTCTTCAGAAGAAGGTGGAGCAATTGTGTGATTCAGGATTGATTCTTCAGGCCTATAAGCTTCTGATACAACTAGCTAGTAGTGGGGTTGCCCCCGACATCATCACTTACAACACTCTAATCGATGGCTTTTGTAAAAGTGGAAACATGGATGGTGCTTTTAAACTCTTCAAGGACATGCAACTGAAAGGGATCACCCCAGATTCTGTTACATACGGGACACTTATTGATGGGCTTCAAAGAGCTGAAAGAGAAGAAGATGCCTTTTTGGTCTTTAATCAAATGGTGAAGAATGGATGCACACCTAGTGCTGAAGTTTACAAATCACTTATGACCTGGTCTTCACGGAATAGGAAGGTTACATTATCTCTTAGTCTTTGGTTGAAGTACCTGAGAAGCCTTCCTAACAGAGATGAGGTAACAATCGAAGCAATAGAGAAAAATTTTAAGGAAGGGCAAATTGAGAAGGCTATCCAAGGCTTACTTGAAATGGATGTACAGTTTAAAAATTTAGATTTAGGACCATACACTATTTTGCTCATTGGTTTGTGTCAGGTGCAAAGAGTGGATGAAGCTCTGAGAATGTTTTCTGTACTTCAGGAGTACAAAGTCAATATCACTCCACCAAGTTGTGTGCACCTGATAGATGGTCTATGCAGAGAAGGGAATTTGGACCTGGCAATAAATATTTTCCATTATACTCTGGAGAGGGGTTTCATGTTAATGCCCGAAATATGCAATAAGCTGCTCAAGTGTCTTCTTCGTTCTCGAGACAAAAAGGGCCATGCCTTTGATCTCGTTCATAGGATGAGAAATTTTGGATATGATCTTGATGCTTGTCTTCACCAGACTACGAAGTTTCTACTACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

769

Amino Acids

86.91

Weight (kDa)

8.35

Isoelectric Point (pI)

34.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	104 - 160	5e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	117 - 156	6.5e-08	PPR repeat family
PPR_2	PF13041	151 - 196	7.5e-11	PPR repeat family
TPR_24	PF23276	154 - 276	1.8e-08	Fungal tetratrico peptide repeats
PPR_long	PF17177	167 - 276	5.7e-11	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	179 - 210	1.3e-09	PPR repeat
PPR	PF01535	185 - 215	9.7e-08	PPR repeat
PPR_2	PF13041	190 - 231	1.3e-14	PPR repeat family
PPR_3	PF13812	206 - 264	1.8e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	213 - 246	4.5e-11	PPR repeat
PPR_2	PF13041	218 - 266	8e-15	PPR repeat family
PPR_1	PF12854	249 - 278	9.2e-11	PPR repeat
PPR_3	PF13812	318 - 369	7.1e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	319 - 351	8.1e-06	PPR repeat
PPR_2	PF13041	322 - 371	7.4e-14	PPR repeat family
PPR_1	PF12854	353 - 380	1.9e-07	PPR repeat
PPR_2	PF13041	357 - 406	4.1e-13	PPR repeat family
PPR_3	PF13812	384 - 437	2.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	389 - 421	2e-13	PPR repeat
PPR_2	PF13041	392 - 427	1.6e-09	PPR repeat family
PPR	PF01535	396 - 425	1e-08	PPR repeat
PPR_1	PF12854	424 - 456	1.1e-09	PPR repeat
PPR_2	PF13041	427 - 456	5.8e-07	PPR repeat family
PPR	PF01535	430 - 456	8.3e-06	PPR repeat
PPR_2	PF13041	487 - 524	4.9e-08	PPR repeat family
PPR_3	PF13812	501 - 554	6.4e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	507 - 539	3.2e-15	PPR repeat
PPR_2	PF13041	510 - 557	1.5e-20	PPR repeat family
PPR	PF01535	513 - 543	1.4e-10	PPR repeat
PPR_3	PF13812	533 - 589	6.6e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	541 - 574	7.2e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016134)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79540
fragaria_vesca	FvH4_3g13661
malus_domestica	MD05G1241300.v1.1
prunus_persica	Prupe.4G124200_v2.0.a1 Prupe.4G124200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0022901
rosa_laevigata	RLG00000032681
rosa_multiflora	Rmu_co8399345.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056430
rosa_rugosa	Rorug05G0075400
rosa_samantha	Rh5AG161900 Rh5BG161100 Rh5CG175600 Rh5DG162300
rosa_wichuraiana	Rw5G014570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1086
AccB1I	GGYRCC	1 cut(s)	54
AccI	GTMKAC	1 cut(s)	255
AccII	CGCG	2 cut(s)	347, 824
AciI	CCGC	7 cut(s)	16, 52, 58, 324, 342, 398, 461
AclWI	GGATC	4 cut(s)	148, 210, 341, 1634
AcoI	YGGCCR	1 cut(s)	130
AcsI	RAATTY	5 cut(s)	1033, 1876, 1942, 2101, 2248
AcuI	CTGAAG	4 cut(s)	1424, 1466, 1767, 2015
AcyI	GRCGYC	1 cut(s)	55
AdeI	CACNNNGTG	1 cut(s)	1742
AfaI	GTAC	6 cut(s)	556, 982, 1823, 1932, 2028, 2039
AfiI	CCNNNNNNNGG	3 cut(s)	51, 181, 655
AflII	CTTAAG	1 cut(s)	1334
AgsI	TTSAA	8 cut(s)	529, 1033, 1252, 1297, 1606, 1673, 1819, 1922
AjnI	CCWGG	4 cut(s)	600, 690, 1769, 2109
Alw21I	GWGCWC	3 cut(s)	302, 315, 2073
Alw26I	GTCTC	2 cut(s)	734, 2199
Alw44I	GTGCAC	1 cut(s)	2069
AlwI	GGATC	4 cut(s)	148, 210, 341, 1634
AlwNI	CAGNNNCTG	4 cut(s)	237, 321, 1010, 1640
Ama87I	CYCGRG	1 cut(s)	2202
AoxI	GGCC	5 cut(s)	130, 183, 204, 1485, 2215
ApaLI	GTGCAC	1 cut(s)	2069
ApeKI	GCWGC	2 cut(s)	160, 2179
ApoI	RAATTY	5 cut(s)	1033, 1876, 1942, 2101, 2248
ArsI	GACNNNNNNTTYG	2 cut(s)	266, 298
Asp700I	GAANNNNTTC	2 cut(s)	1381, 2020
AspLEI	GCGC	5 cut(s)	57, 347, 715, 826, 925
AspS9I	GGNCC	4 cut(s)	204, 1955, 2107, 2215
AsuHPI	GGTGA	6 cut(s)	100, 490, 1055, 1621, 1732, 2270
AsuII	TTCGAA	1 cut(s)	1157
AvaI	CYCGRG	1 cut(s)	2202
AvaII	GGWCC	2 cut(s)	1955, 2107
BaeGI	GKGCMC	2 cut(s)	706, 2073
BanI	GGYRCC	1 cut(s)	54
BanII	GRGCYC	1 cut(s)	315
BauI	CACGAG	1 cut(s)	707
BbsI	GAAGAC	5 cut(s)	187, 1430, 1767, 2183, 2267
Bbv12I	GWGCWC	3 cut(s)	302, 315, 2073
BbvI	GCAGC	2 cut(s)	172, 2166
BceAI	ACGGC	1 cut(s)	145
BciT130I	CCWGG	4 cut(s)	602, 692, 1771, 2111
BclI	TGATCA	2 cut(s)	360, 570
BcoDI	GTCTC	2 cut(s)	734, 2199
BfaI	CTAG	6 cut(s)	549, 591, 906, 1508, 1512, 1740
BfoI	RGCGCY	2 cut(s)	58, 926
BfrI	CTTAAG	1 cut(s)	1334
BglI	GCCNNNNNGGC	1 cut(s)	395
BisI	GCNGC	5 cut(s)	53, 58, 161, 343, 2180
BlsI	GCNGC	5 cut(s)	54, 59, 162, 344, 2181
BmcAI	AGTACT	1 cut(s)	556
Bme1390I	CCNGG	4 cut(s)	602, 692, 1771, 2111
Bme18I	GGWCC	2 cut(s)	1955, 2107
BmeT110I	CYCGRG	1 cut(s)	2202
BmgT120I	GGNCC	4 cut(s)	204, 1955, 2107, 2215
BmiI	GGNNCC	2 cut(s)	56, 1068
BmrFI	CCNGG	4 cut(s)	602, 692, 1771, 2111
BmrI	ACTGGG	1 cut(s)	753
BmsI	GCATC	7 cut(s)	595, 1015, 1063, 1330, 1684, 1721, 2257
BmuI	ACTGGG	1 cut(s)	753
BpiI	GAAGAC	5 cut(s)	187, 1430, 1767, 2183, 2267
BpmI	CTGGAG	2 cut(s)	316, 2159
Bpu10I	CCTNAGC	1 cut(s)	314
Bpu14I	TTCGAA	1 cut(s)	1157
BpuEI	CTTGAG	3 cut(s)	1169, 1389, 2168
Bsa29I	ATCGAT	1 cut(s)	1557
BsaHI	GRCGYC	1 cut(s)	55
BsaJI	CCNNGG	4 cut(s)	47, 219, 600, 1909
BsaXI	ACNNNNNCTCC	4 cut(s)	731, 761, 1445, 1475
Bsc4I	CCNNNNNNNGG	3 cut(s)	51, 181, 655
Bse1I	ACTGG	3 cut(s)	186, 299, 748
Bse3DI	GCAATG	1 cut(s)	608
BseBI	CCWGG	4 cut(s)	602, 692, 1771, 2111
BseCI	ATCGAT	1 cut(s)	1557
BseDI	CCNNGG	4 cut(s)	47, 219, 600, 1909
BseLI	CCNNNNNNNGG	3 cut(s)	51, 181, 655
BseMI	GCAATG	1 cut(s)	608
BseMII	CTCAG	3 cut(s)	328, 1817, 2003
BseNI	ACTGG	3 cut(s)	186, 299, 748
BseRI	GAGGAG	3 cut(s)	95, 104, 437
BseSI	GKGCMC	2 cut(s)	706, 2073
BseXI	GCAGC	2 cut(s)	172, 2166
BseYI	CCCAGC	1 cut(s)	379
BsgI	GTGCAG	2 cut(s)	1168, 1234
Bsh1236I	CGCG	2 cut(s)	347, 824
BshFI	GGCC	5 cut(s)	132, 185, 206, 1487, 2217
BshNI	GGYRCC	1 cut(s)	54
BshVI	ATCGAT	1 cut(s)	1557
BsiHKAI	GWGCWC	3 cut(s)	302, 315, 2073
BsiHKCI	CYCGRG	1 cut(s)	2202
BsiSI	CCGG	1 cut(s)	440
BslFI	GGGAC	2 cut(s)	332, 1666
BslI	CCNNNNNNNGG	3 cut(s)	51, 181, 655
BsmAI	GTCTC	2 cut(s)	734, 2199
BsmFI	GGGAC	2 cut(s)	332, 1666
BsmI	GAATGC	1 cut(s)	391
BsnI	GGCC	5 cut(s)	132, 185, 206, 1487, 2217
BsoBI	CYCGRG	1 cut(s)	2202
Bsp119I	TTCGAA	1 cut(s)	1157
Bsp1286I	GDGCHC	4 cut(s)	302, 315, 706, 2073
Bsp1407I	TGTACA	1 cut(s)	1930
Bsp19I	CCATGG	1 cut(s)	47
BspACI	CCGC	7 cut(s)	16, 52, 58, 324, 342, 398, 461
BspANI	GGCC	5 cut(s)	132, 185, 206, 1487, 2217
BspCNI	CTCAG	3 cut(s)	327, 1818, 2004
BspDI	ATCGAT	1 cut(s)	1557
BspFNI	CGCG	2 cut(s)	347, 824
BspLI	GGNNCC	2 cut(s)	56, 1068
BspPI	GGATC	4 cut(s)	148, 210, 341, 1634
BspT104I	TTCGAA	1 cut(s)	1157
BspT107I	GGYRCC	1 cut(s)	54
BspTI	CTTAAG	1 cut(s)	1334
BsrDI	GCAATG	1 cut(s)	608
BsrGI	TGTACA	1 cut(s)	1930
BsrI	ACTGG	3 cut(s)	186, 299, 748
BssECI	CCNNGG	4 cut(s)	47, 219, 600, 1909
BssNI	GRCGYC	1 cut(s)	55
BssSI	CACGAG	1 cut(s)	707
BssT1I	CCWWGG	3 cut(s)	47, 219, 1909
Bst2BI	CACGAG	1 cut(s)	707
Bst2UI	CCWGG	4 cut(s)	602, 692, 1771, 2111
Bst4CI	ACNGT	5 cut(s)	94, 282, 658, 1082, 1935
Bst6I	CTCTTC	2 cut(s)	692, 1607
BstACI	GRCGYC	1 cut(s)	55
BstAFI	CTTAAG	1 cut(s)	1334
BstAUI	TGTACA	1 cut(s)	1930
BstBI	TTCGAA	1 cut(s)	1157
BstC8I	GCNNGC	2 cut(s)	1012, 1183
BstDEI	CTNAG	6 cut(s)	314, 539, 1329, 1805, 1826, 2012
BstDSI	CCRYGG	1 cut(s)	47
BstFNI	CGCG	2 cut(s)	347, 824
BstH2I	RGCGCY	2 cut(s)	58, 926
BstHHI	GCGC	5 cut(s)	57, 347, 715, 826, 925
BstMAI	GTCTC	2 cut(s)	734, 2199
BstMWI	GCNNNNNNNGC	4 cut(s)	395, 404, 701, 710
BstNI	CCWGG	4 cut(s)	602, 692, 1771, 2111
BstNSI	RCATGY	3 cut(s)	262, 1351, 1615
BstSCI	CCNGG	4 cut(s)	600, 690, 1769, 2109
BstSLI	GKGCMC	2 cut(s)	706, 2073
BstUI	CGCG	2 cut(s)	347, 824
BstV1I	GCAGC	2 cut(s)	172, 2166
BstV2I	GAAGAC	5 cut(s)	187, 1430, 1767, 2183, 2267
BstX2I	RGATCY	1 cut(s)	215
BstYI	RGATCY	1 cut(s)	215
Bsu15I	ATCGAT	1 cut(s)	1557
BsuRI	GGCC	5 cut(s)	132, 185, 206, 1487, 2217
BsuTUI	ATCGAT	1 cut(s)	1557
BtgI	CCRYGG	1 cut(s)	47
BtsI	GCAGTG	1 cut(s)	1417
BtsIMutI	CAGTG	1 cut(s)	1417
Cac8I	GCNNGC	2 cut(s)	1012, 1183
CaiI	CAGNNNCTG	4 cut(s)	237, 321, 1010, 1640
CfoI	GCGC	5 cut(s)	57, 347, 715, 826, 925
Cfr13I	GGNCC	4 cut(s)	204, 1955, 2107, 2215
ClaI	ATCGAT	1 cut(s)	1557
CseI	GACGC	1 cut(s)	1016
CsiI	ACCWGGT	1 cut(s)	1769
Csp6I	GTAC	6 cut(s)	555, 981, 1822, 1931, 2027, 2038
CviAII	CATG	9 cut(s)	48, 259, 773, 1281, 1348, 1582, 1612, 2152, 2219
CviQI	GTAC	6 cut(s)	555, 981, 1822, 1931, 2027, 2038
DdeI	CTNAG	6 cut(s)	314, 539, 1329, 1805, 1826, 2012
DinI	GGCGCC	1 cut(s)	56
DraI	TTTAAA	2 cut(s)	1597, 1939
DraIII	CACNNNGTG	1 cut(s)	1742
EaeI	YGGCCR	1 cut(s)	130
Eam1104I	CTCTTC	2 cut(s)	692, 1607
EarI	CTCTTC	2 cut(s)	692, 1607
EciI	GGCGGA	1 cut(s)	5
Ecl136II	GAGCTC	1 cut(s)	313
Eco130I	CCWWGG	3 cut(s)	47, 219, 1909
Eco147I	AGGCCT	1 cut(s)	1487
Eco24I	GRGCYC	1 cut(s)	315
Eco47I	GGWCC	2 cut(s)	1955, 2107
Eco53kI	GAGCTC	1 cut(s)	313
Eco57I	CTGAAG	4 cut(s)	1424, 1466, 1767, 2015
Eco88I	CYCGRG	1 cut(s)	2202
EcoICRI	GAGCTC	1 cut(s)	313
EcoRII	CCWGG	4 cut(s)	600, 690, 1769, 2109
EcoT14I	CCWWGG	3 cut(s)	47, 219, 1909
EcoT22I	ATGCAT	1 cut(s)	610
EcoT38I	GRGCYC	1 cut(s)	315
EgeI	GGCGCC	1 cut(s)	56
EheI	GGCGCC	1 cut(s)	56
ErhI	CCWWGG	3 cut(s)	47, 219, 1909
FaeI	CATG	9 cut(s)	51, 262, 776, 1284, 1351, 1585, 1615, 2155, 2222
FalI	AAGNNNNNCTT	4 cut(s)	1366, 1398, 1902, 1934
FaqI	GGGAC	2 cut(s)	332, 1666
FatI	CATG	9 cut(s)	47, 258, 772, 1280, 1347, 1581, 1611, 2151, 2218
FauI	CCCGC	1 cut(s)	317
FbaI	TGATCA	2 cut(s)	360, 570
FblI	GTMKAC	1 cut(s)	255
Fnu4HI	GCNGC	5 cut(s)	53, 58, 161, 343, 2180
FriOI	GRGCYC	1 cut(s)	315
Fsp4HI	GCNGC	5 cut(s)	53, 58, 161, 343, 2180
FspBI	CTAG	6 cut(s)	549, 591, 906, 1508, 1512, 1740
GlaI	GCGC	5 cut(s)	56, 346, 714, 825, 924
GluI	GCNGC	5 cut(s)	53, 58, 161, 343, 2180
GsaI	CCCAGC	1 cut(s)	383
GsuI	CTGGAG	2 cut(s)	316, 2159
HaeII	RGCGCY	2 cut(s)	58, 926
HaeIII	GGCC	5 cut(s)	132, 185, 206, 1487, 2217
HapII	CCGG	1 cut(s)	440
HgaI	GACGC	1 cut(s)	1016
HhaI	GCGC	5 cut(s)	57, 347, 715, 826, 925
Hin1I	GRCGYC	1 cut(s)	55
Hin1II	CATG	9 cut(s)	51, 262, 776, 1284, 1351, 1585, 1615, 2155, 2222
Hin6I	GCGC	5 cut(s)	55, 345, 713, 824, 923
HinP1I	GCGC	5 cut(s)	55, 345, 713, 824, 923
HincII	GTYRAC	2 cut(s)	97, 256
HindII	GTYRAC	2 cut(s)	97, 256
HindIII	AAGCTT	3 cut(s)	1265, 1331, 1493
HinfI	GANTC	4 cut(s)	1435, 1466, 1477, 1637
HpaII	CCGG	1 cut(s)	440
HphI	GGTGA	6 cut(s)	100, 490, 1055, 1621, 1732, 2270
Hpy166II	GTNNAC	5 cut(s)	97, 256, 981, 1753, 2071
Hpy188III	TCNNGA	8 cut(s)	1070, 1148, 1470, 2033, 2138, 2202, 2204, 2264
Hpy8I	GTNNAC	5 cut(s)	97, 256, 981, 1753, 2071
Hpy99I	CGWCG	2 cut(s)	131, 257
HpyAV	CCTTC	9 cut(s)	388, 1303, 1392, 1441, 1783, 1844, 1880, 1894, 2090
HpyCH4III	ACNGT	5 cut(s)	94, 282, 658, 1082, 1935
HpyCH4IV	ACGT	1 cut(s)	969
HpyF10VI	GCNNNNNNNGC	4 cut(s)	395, 404, 701, 710
HpyF3I	CTNAG	6 cut(s)	314, 539, 1329, 1805, 1826, 2012
HpySE526I	ACGT	1 cut(s)	969
Hsp92I	GRCGYC	1 cut(s)	55
Hsp92II	CATG	9 cut(s)	51, 262, 776, 1284, 1351, 1585, 1615, 2155, 2222
HspAI	GCGC	5 cut(s)	55, 345, 713, 824, 923
KasI	GGCGCC	1 cut(s)	54
Ksp22I	TGATCA	2 cut(s)	360, 570
LmnI	GCTCC	4 cut(s)	18, 297, 310, 1453
Lsp1109I	GCAGC	2 cut(s)	172, 2166
LweI	GCATC	7 cut(s)	595, 1015, 1063, 1330, 1684, 1721, 2257
MabI	ACCWGGT	1 cut(s)	1769
MaeI	CTAG	6 cut(s)	549, 591, 906, 1508, 1512, 1740
MaeII	ACGT	1 cut(s)	969
MaeIII	GTNAC	7 cut(s)	239, 658, 763, 1288, 1642, 1792, 1852
MfeI	CAATTG	1 cut(s)	1457
MflI	RGATCY	1 cut(s)	215
MhlI	GDGCHC	4 cut(s)	302, 315, 706, 2073
Mly113I	GGCGCC	1 cut(s)	55
MlyI	GAGTC	1 cut(s)	1444
MmeI	TCCRAC	1 cut(s)	150
Mph1103I	ATGCAT	1 cut(s)	610
MroXI	GAANNNNTTC	2 cut(s)	1381, 2020
MseI	TTAA	9 cut(s)	267, 993, 1020, 1335, 1596, 1710, 1881, 1938, 2156
MslI	CAYNNNNRTG	1 cut(s)	2156
MspCI	CTTAAG	1 cut(s)	1334
MspI	CCGG	1 cut(s)	440
MspR9I	CCNGG	4 cut(s)	602, 692, 1771, 2111
MunI	CAATTG	1 cut(s)	1457
Mva1269I	GAATGC	1 cut(s)	391
MvaI	CCWGG	4 cut(s)	602, 692, 1771, 2111
MvnI	CGCG	2 cut(s)	347, 824
MwoI	GCNNNNNNNGC	4 cut(s)	395, 404, 701, 710
NarI	GGCGCC	1 cut(s)	55
NcoI	CCATGG	1 cut(s)	47
NlaIII	CATG	9 cut(s)	51, 262, 776, 1284, 1351, 1585, 1615, 2155, 2222
NlaIV	GGNNCC	2 cut(s)	56, 1068
NmeAIII	GCCGAG	1 cut(s)	415
NmuCI	GTSAC	1 cut(s)	239
NsiI	ATGCAT	1 cut(s)	610
NspI	RCATGY	3 cut(s)	262, 1351, 1615
NspV	TTCGAA	1 cut(s)	1157
PaeR7I	CTCGAG	1 cut(s)	2202
PceI	AGGCCT	1 cut(s)	1487
PcsI	WCGNNNNNNNCGW	3 cut(s)	123, 261, 270
PctI	GAATGC	1 cut(s)	391
PdmI	GAANNNNTTC	2 cut(s)	1381, 2020
PfeI	GAWTC	3 cut(s)	1466, 1477, 1637
PflFI	GACNNNGTC	1 cut(s)	1771
PkrI	GCNGC	5 cut(s)	54, 59, 162, 344, 2181
PleI	GAGTC	1 cut(s)	1443
PluTI	GGCGCC	1 cut(s)	58
PpsI	GAGTC	1 cut(s)	1443
PsiI	TTATAA	1 cut(s)	1086
Psp124BI	GAGCTC	1 cut(s)	315
Psp6I	CCWGG	4 cut(s)	600, 690, 1769, 2109
PspFI	CCCAGC	1 cut(s)	379
PspGI	CCWGG	4 cut(s)	600, 690, 1769, 2109
PspN4I	GGNNCC	2 cut(s)	56, 1068
PspPI	GGNCC	4 cut(s)	204, 1955, 2107, 2215
PstNI	CAGNNNCTG	4 cut(s)	237, 321, 1010, 1640
PsuI	RGATCY	1 cut(s)	215
PsyI	GACNNNGTC	1 cut(s)	1771
RsaI	GTAC	6 cut(s)	556, 982, 1823, 1932, 2028, 2039
RsaNI	GTAC	6 cut(s)	555, 981, 1822, 1931, 2027, 2038
RseI	CAYNNNNRTG	1 cut(s)	2156
SacI	GAGCTC	1 cut(s)	315
SalI	GTCGAC	1 cut(s)	254
SaqAI	TTAA	9 cut(s)	267, 993, 1020, 1335, 1596, 1710, 1881, 1938, 2156
SatI	GCNGC	5 cut(s)	53, 58, 161, 343, 2180
Sau96I	GGNCC	4 cut(s)	204, 1955, 2107, 2215
ScaI	AGTACT	1 cut(s)	556
SchI	GAGTC	1 cut(s)	1444
ScrFI	CCNGG	4 cut(s)	602, 692, 1771, 2111
SduI	GDGCHC	4 cut(s)	302, 315, 706, 2073
SexAI	ACCWGGT	1 cut(s)	1769
SfaNI	GCATC	7 cut(s)	595, 1015, 1063, 1330, 1684, 1721, 2257
SfoI	GGCGCC	1 cut(s)	56
Sfr274I	CTCGAG	1 cut(s)	2202
SfuI	TTCGAA	1 cut(s)	1157
SinI	GGWCC	2 cut(s)	1955, 2107
SlaI	CTCGAG	1 cut(s)	2202
SmiMI	CAYNNNNRTG	1 cut(s)	2156
SmlI	CTYRAG	5 cut(s)	1148, 1334, 1404, 2183, 2202
SmoI	CTYRAG	5 cut(s)	1148, 1334, 1404, 2183, 2202
SseBI	AGGCCT	1 cut(s)	1487
SsiI	CCGC	7 cut(s)	16, 52, 58, 324, 342, 398, 461
SspDI	GGCGCC	1 cut(s)	54
SspI	AATATT	2 cut(s)	760, 2122
SspMI	CTAG	6 cut(s)	549, 591, 906, 1508, 1512, 1740
SstI	GAGCTC	1 cut(s)	315
StuI	AGGCCT	1 cut(s)	1487
StyD4I	CCNGG	4 cut(s)	600, 690, 1769, 2109
StyI	CCWWGG	3 cut(s)	47, 219, 1909
TaaI	ACNGT	5 cut(s)	94, 282, 658, 1082, 1935
TaiI	ACGT	1 cut(s)	972
TaqI	TCGA	9 cut(s)	117, 255, 285, 1135, 1157, 1417, 1557, 1860, 2203
TatI	WGTACW	5 cut(s)	554, 980, 1930, 2026, 2037
TauI	GCSGC	3 cut(s)	55, 60, 345
TfiI	GAWTC	3 cut(s)	1466, 1477, 1637
Tru1I	TTAA	9 cut(s)	267, 993, 1020, 1335, 1596, 1710, 1881, 1938, 2156
Tru9I	TTAA	9 cut(s)	267, 993, 1020, 1335, 1596, 1710, 1881, 1938, 2156
TscAI	CASTG	1 cut(s)	1417
TseFI	GTSAC	1 cut(s)	239
TseI	GCWGC	2 cut(s)	160, 2179
Tsp45I	GTSAC	1 cut(s)	239
TspDTI	ATGAA	8 cut(s)	17, 22, 187, 1143, 1374, 2019, 2140, 2225
TspGWI	ACGGA	1 cut(s)	1795
TspRI	CASTG	1 cut(s)	1417
Tth111I	GACNNNGTC	1 cut(s)	1771
Vha464I	CTTAAG	1 cut(s)	1334
VneI	GTGCAC	1 cut(s)	2069
VpaK11BI	GGWCC	2 cut(s)	1955, 2107
XapI	RAATTY	5 cut(s)	1033, 1876, 1942, 2101, 2248
XceI	RCATGY	3 cut(s)	262, 1351, 1615
XcmI	CCANNNNNNNNNTGG	1 cut(s)	178
XhoI	CTCGAG	1 cut(s)	2202
XmiI	GTMKAC	1 cut(s)	255
XmnI	GAANNNNTTC	2 cut(s)	1381, 2020
XspI	CTAG	6 cut(s)	549, 591, 906, 1508, 1512, 1740
ZrmI	AGTACT	1 cut(s)	556
Zsp2I	ATGCAT	1 cut(s)	610

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.