Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0016134

Physical Location & Seq

Genomic Coordinates Reverse (-)

78186895 .. 78195999

Gene Structure 9105 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00056430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGGGGATCTTTACCCGCACCGAAGACCGGGCCGCCTTCTCCAACAGGGTCCTTCACCTTGTCTTGGAGCTTATCAATCTGAACGCTTCCCTGACGATATTAACTTTGCTCTGCAATGTTGATGCTGGAGACACATGTTCACTACTCAGTTTACTTTTAGTCCAGAACTTGAGACCTATGAGCAGGTACAGATGTTTAAACTGGGAATGCCAACAAGAGATACTATGCAGCCCCTCAACTTGCAGAATAAAAGCTAATTTGCTCGTATATTGTTGTAACATCAACTTTATGTTGTACTTTAGAACCACAAATCCTCTCAAATTTTCATTAATCAAAATATATTATGGTGCTTTCCTGAATTTCGTCGGTGCTCATCAGATTGCGGGATTCGAATCGGAAGGCGGTGGAGGCTTTTCAAGGATGAAAGATTTCGATTGTAAGCCTGATGTTTATACCTACAATGCGGTTTTATATGTTTTGGTGAGGAAAGAAGTGTTTTTGTTAGCTTTAGCTGTGTATAACCAAATGTTGAAGTGTAATCTTAGTCCCAGTAGGACTACTTATAGCATTTTGATCAATGGGTTTTGCAAGAGTAGGAAAACGCAGGATGCGATTCAGATGTTTGATGAGATGACACAGAGAGGGATTGCCCCCAATACCGTTACTTATACTATTATCATTTCCGGGTTGTGCAAGGCAAAGAGGGCCCATGATGCGCATAGGCTGGTCGATACGATGAAGGAAACTGGGTGTACTCCTGATGTGATTACTTACCATGCTTTGCTTGATGGGTATTGTAAGTTAGGTAGGCTTGATGAAGCTTACGCGCTCGTGAGGTTGTTGCAGAGGGATGGTTATGTTCTTGGAGTGGAGGGATATAGTTCCTTGATTTTGGGTTTGTTTAGAGCTAGGAGGTTTGATGAGGCACATGAGTTGTATGGGAAGTTGATCGACGAGGGGATTAAGCTTGACGTTATCTTGTGTACTATATTGATTAAGGGGCTGGCAGATGCTGGCAGGGTTACGGATGCTTTAAACTTCTTGAGTGAGATGAGTAAGAAAGGTTTGGTTCCAGATGCATACTGCTATAATGCTGTCATCAAAGGGTTCTGCGATTTAGGTCTTTTGGATGAAGCTCGATCTCTCCATCTTGAAATTTCGAAGCAAGATTGCTTCCCTAATGCCTGCACTTGCACCATTCTCATTTGTGGTATGTGCAGGAATGGGCTGATAGGGGAAGCAGCACAGATTTTCAATGAGATGGAGAAGCTTGGATGTGTTCCTTCTGTTGTAACCTTCAATGCTCTTATTGATGGGCTTTGTAAGGCTTCTAAGCTTGAGGATGCACAGTTGCTGTTTTACAAGATGGAGATAGGAAAAAAACCTTCATTATTTCTTCGTCTTTCTCAAGGTAGTGATCGGGTTATTGATAGTGCGAGTCTTCAAAAGAAGGTGGAGCAATTGTGCGAGTCAGGATTGATTCTTCAGGCCTATAAACTTCTGATGCAGCTAGCTAGTAGTGGGGTTGCCCCCGACGTCATCACTTACAACACTCTAATCAATGGATTTTGCAAAAATGGAAACATAGATGGTGCTTTCAAACTCTTCAAGGATATGCAATTGAAAGGGATCACCCCAGATTCTGTTACATATGGAACACTTATAGATGGACTTCAAAGGGTTGAAAGAGAAGAAGATGCCTTTGTGGTCTTTAATCAAATGGTGAAGAATGGATGCACACCTAGCTCTGAAGTTTACAAATCACTGATGACTTGGTCTTGCAGGTATAGCAAGGTTTCGGTAGCTGTTAGTCTTTGGTTGAAGTACCTGAGCAGCCTTCCTCACAGAGAAGAGGAAGCAATCAAAGCAATAGAGGAAAATTTTAAAGAGGGGCAAATTGAGAAGGCGATCCAAGGCTTACTTGAAATGGATGTAAAGTTTAAAGATTTAGATTTAGGACCGTACACTATTTTGCTCATTGGTTTGTGTCAGGTGCAAAGAGTGGGTGAAGCTCTGAGAATGTTCTCTGTACTTCAGGAGTACAAAGTCAATGTCACTCCACCAAGTTGTGTGCACTTGATAAATGGTCTCTGCAAAGAAGGGAATTTGGACCTGGCAATAAATGTTTTCCATTATACTCTGGAGAGGGGTTTCATATTAATGCCTGAAATATGCAATAAGCTGCTCAAGTGTCTTCTTCGTTCCCGAGACAAAAAGGACCGTGCCTTTGATCTCATTCGTAGGATGAGAAATTTTGGATATGATCTTGATGCTTGTCTTCACCAGACTACAAAGTTACTTCTACAATTTCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

771

Amino Acids

86.68

Weight (kDa)

8.04

Isoelectric Point (pI)

32.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	140 - 195	4e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	148 - 197	2.4e-13	PPR repeat family
TPR_24	PF23276	156 - 274	3e-07	Fungal tetratrico peptide repeats
PPR_long	PF17177	168 - 279	9.6e-13	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	180 - 212	3.1e-10	PPR repeat
PPR	PF01535	187 - 216	5.5e-08	PPR repeat
PPR_2	PF13041	191 - 232	4e-16	PPR repeat family
PPR_3	PF13812	207 - 265	8.4e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	214 - 247	2.4e-11	PPR repeat
PPR_2	PF13041	219 - 267	1.7e-16	PPR repeat family
PPR_1	PF12854	250 - 279	5.8e-11	PPR repeat
PPR_2	PF13041	290 - 336	4.7e-06	PPR repeat family
PPR_3	PF13812	317 - 370	3.1e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	324 - 372	3.6e-13	PPR repeat family
PPR_1	PF12854	354 - 381	1.9e-07	PPR repeat
PPR_2	PF13041	358 - 407	1.5e-11	PPR repeat family
PPR_3	PF13812	385 - 438	3.2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	390 - 422	5.9e-09	PPR repeat
PPR_2	PF13041	393 - 442	6.3e-17	PPR repeat family
PPR	PF01535	397 - 426	1.2e-06	PPR repeat
PPR_1	PF12854	425 - 457	2.4e-11	PPR repeat
PPR_2	PF13041	428 - 457	7.3e-06	PPR repeat family
PPR	PF01535	431 - 457	9.4e-06	PPR repeat
PPR_2	PF13041	488 - 525	7.4e-09	PPR repeat family
PPR_long	PF17177	494 - 629	1.3e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	500 - 555	3.2e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	508 - 540	1.4e-16	PPR repeat
PPR_2	PF13041	511 - 558	4.7e-21	PPR repeat family
PPR	PF01535	514 - 544	9e-11	PPR repeat
PPR_3	PF13812	534 - 590	7.2e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	542 - 575	2.3e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016134)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79540
fragaria_vesca	FvH4_3g13661
malus_domestica	MD05G1241300.v1.1
prunus_persica	Prupe.4G124200_v2.0.a1 Prupe.4G124200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0022901
rosa_laevigata	RLG00000032681
rosa_multiflora	Rmu_co8399345.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056430
rosa_rugosa	Rorug05G0075400
rosa_samantha	Rh5AG161900 Rh5BG161100 Rh5CG175600 Rh5DG162300
rosa_wichuraiana	Rw5G014570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1539
Acc16I	TGCGCA	1 cut(s)	717
Acc36I	ACCTGC	2 cut(s)	174, 1773
AccII	CGCG	1 cut(s)	827
AciI	CCGC	5 cut(s)	16, 33, 383, 402, 464
AclWI	GGATC	3 cut(s)	14, 1637, 1903
AcsI	RAATTY	6 cut(s)	320, 358, 1155, 1879, 2104, 2251
AcuI	CTGAAG	3 cut(s)	1469, 1770, 2018
AcyI	GRCGYC	1 cut(s)	1536
AfaI	GTAC	8 cut(s)	188, 296, 754, 985, 1826, 1964, 2031, 2042
AfiI	CCNNNNNNNGG	2 cut(s)	27, 64
AflIII	ACRYGT	1 cut(s)	134
AjnI	CCWGG	1 cut(s)	2112
AloI	GAACNNNNNNTCC	2 cut(s)	295, 327
Alw21I	GWGCWC	2 cut(s)	373, 2076
Alw26I	GTCTC	4 cut(s)	123, 166, 2093, 2202
Alw44I	GTGCAC	1 cut(s)	2072
AlwI	GGATC	3 cut(s)	14, 1637, 1903
AlwNI	CAGNNNCTG	3 cut(s)	1013, 1354, 1643
Ama87I	CYCGRG	1 cut(s)	2205
AoxI	GGCC	3 cut(s)	30, 705, 1488
ApaI	GGGCCC	1 cut(s)	709
ApaLI	GTGCAC	1 cut(s)	2072
ApeKI	GCWGC	5 cut(s)	228, 1241, 1507, 1833, 2182
ApoI	RAATTY	6 cut(s)	320, 358, 1155, 1879, 2104, 2251
ArsI	GACNNNNNNTTYG	4 cut(s)	2037, 2069, 2210, 2242
AseI	ATTAAT	2 cut(s)	329, 2159
Asp700I	GAANNNNTTC	1 cut(s)	428
AspLEI	GCGC	2 cut(s)	718, 829
AspS9I	GGNCC	7 cut(s)	30, 49, 705, 706, 1958, 2110, 2218
AsuC2I	CCSGG	2 cut(s)	29, 685
AsuHPI	GGTGA	6 cut(s)	47, 493, 1624, 1735, 2018, 2273
AsuII	TTCGAA	2 cut(s)	390, 1160
AsuNHI	GCTAGC	1 cut(s)	1510
AvaI	CYCGRG	1 cut(s)	2205
AvaII	GGWCC	4 cut(s)	49, 1958, 2110, 2218
BaeGI	GKGCMC	2 cut(s)	709, 2076
BaeI	ACNNNNGTAYC	2 cut(s)	178, 211
BanII	GRGCYC	1 cut(s)	709
BauI	CACGAG	1 cut(s)	830
BbsI	GAAGAC	4 cut(s)	30, 1433, 2186, 2270
Bbv12I	GWGCWC	2 cut(s)	373, 2076
BbvI	GCAGC	5 cut(s)	240, 1253, 1519, 1845, 2169
BccI	CCATC	8 cut(s)	782, 845, 1155, 1255, 1307, 1360, 1583, 1661
BciT130I	CCWGG	1 cut(s)	2114
BclI	TGATCA	1 cut(s)	573
BcnI	CCSGG	2 cut(s)	29, 685
BcoDI	GTCTC	4 cut(s)	123, 166, 2093, 2202
BfaI	CTAG	5 cut(s)	909, 1511, 1515, 1743, 2314
BfuAI	ACCTGC	2 cut(s)	174, 1773
BisI	GCNGC	6 cut(s)	33, 229, 1242, 1508, 1834, 2183
BlsI	GCNGC	6 cut(s)	34, 230, 1243, 1509, 1835, 2184
Bme1390I	CCNGG	3 cut(s)	29, 685, 2114
Bme18I	GGWCC	4 cut(s)	49, 1958, 2110, 2218
BmeT110I	CYCGRG	1 cut(s)	2205
BmgT120I	GGNCC	7 cut(s)	30, 49, 705, 706, 1958, 2110, 2218
BmiI	GGNNCC	3 cut(s)	50, 707, 1071
BmrFI	CCNGG	3 cut(s)	29, 685, 2114
BmrI	ACTGGG	3 cut(s)	211, 543, 756
BmtI	GCTAGC	1 cut(s)	1514
BmuI	ACTGGG	3 cut(s)	211, 543, 756
BpiI	GAAGAC	4 cut(s)	30, 1433, 2186, 2270
BpmI	CTGGAG	2 cut(s)	147, 2162
Bpu10I	CCTNAGC	1 cut(s)	1829
Bpu14I	TTCGAA	2 cut(s)	390, 1160
BpuEI	CTTGAG	5 cut(s)	190, 1063, 1358, 1392, 2171
BpuMI	CCSGG	2 cut(s)	29, 685
BsaBI	GATNNNNATC	1 cut(s)	391
BsaHI	GRCGYC	1 cut(s)	1536
BsaI	GGTCTC	2 cut(s)	166, 2093
BsaJI	CCNNGG	1 cut(s)	1912
BsaXI	ACNNNNNCTCC	2 cut(s)	1448, 1478
Bsc4I	CCNNNNNNNGG	2 cut(s)	27, 64
Bse1I	ACTGG	3 cut(s)	206, 549, 751
Bse3DI	GCAATG	1 cut(s)	121
Bse8I	GATNNNNATC	1 cut(s)	391
BseBI	CCWGG	1 cut(s)	2114
BseDI	CCNNGG	1 cut(s)	1912
BseJI	GATNNNNATC	1 cut(s)	391
BseLI	CCNNNNNNNGG	2 cut(s)	27, 64
BseMI	GCAATG	1 cut(s)	121
BseMII	CTCAG	3 cut(s)	160, 1820, 2006
BseNI	ACTGG	3 cut(s)	206, 549, 751
BseSI	GKGCMC	2 cut(s)	709, 2076
BseXI	GCAGC	5 cut(s)	240, 1253, 1519, 1845, 2169
BsgI	GTGCAG	2 cut(s)	1171, 1237
Bsh1236I	CGCG	1 cut(s)	827
BshFI	GGCC	3 cut(s)	32, 707, 1490
BsiHKAI	GWGCWC	2 cut(s)	373, 2076
BsiHKCI	CYCGRG	1 cut(s)	2205
BsiSI	CCGG	2 cut(s)	28, 684
BslFI	GGGAC	1 cut(s)	531
BslI	CCNNNNNNNGG	2 cut(s)	27, 64
BsmAI	GTCTC	4 cut(s)	123, 166, 2093, 2202
BsmFI	GGGAC	1 cut(s)	531
BsmI	GAATGC	1 cut(s)	212
BsnI	GGCC	3 cut(s)	32, 707, 1490
Bso31I	GGTCTC	2 cut(s)	166, 2093
BsoBI	CYCGRG	1 cut(s)	2205
Bsp119I	TTCGAA	2 cut(s)	390, 1160
Bsp120I	GGGCCC	1 cut(s)	705
Bsp1286I	GDGCHC	3 cut(s)	373, 709, 2076
Bsp143I	GATC	9 cut(s)	6, 573, 948, 1139, 1417, 1629, 1908, 2230, 2263
BspACI	CCGC	5 cut(s)	16, 33, 383, 402, 464
BspANI	GGCC	3 cut(s)	32, 707, 1490
BspCNI	CTCAG	3 cut(s)	159, 1821, 2007
BspFNI	CGCG	1 cut(s)	827
BspLI	GGNNCC	3 cut(s)	50, 707, 1071
BspMI	ACCTGC	2 cut(s)	174, 1773
BspOI	GCTAGC	1 cut(s)	1514
BspPI	GGATC	3 cut(s)	14, 1637, 1903
BspT104I	TTCGAA	2 cut(s)	390, 1160
BspTNI	GGTCTC	2 cut(s)	166, 2093
BsrDI	GCAATG	1 cut(s)	121
BsrI	ACTGG	3 cut(s)	206, 549, 751
BssECI	CCNNGG	1 cut(s)	1912
BssMI	GATC	9 cut(s)	6, 573, 948, 1139, 1417, 1629, 1908, 2230, 2263
BssNI	GRCGYC	1 cut(s)	1536
BssSI	CACGAG	1 cut(s)	830
BssT1I	CCWWGG	1 cut(s)	1912
Bst2BI	CACGAG	1 cut(s)	830
Bst2UI	CCWGG	1 cut(s)	2114
Bst4CI	ACNGT	4 cut(s)	661, 1350, 1962, 2222
Bst6I	CTCTTC	2 cut(s)	1610, 1845
BstACI	GRCGYC	1 cut(s)	1536
BstBI	TTCGAA	2 cut(s)	390, 1160
BstC8I	GCNNGC	4 cut(s)	1005, 1015, 1186, 1512
BstDEI	CTNAG	5 cut(s)	146, 542, 1332, 1829, 2015
BstFNI	CGCG	1 cut(s)	827
BstHHI	GCGC	2 cut(s)	718, 829
BstKTI	GATC	9 cut(s)	9, 576, 951, 1142, 1420, 1632, 1911, 2233, 2266
BstMAI	GTCTC	4 cut(s)	123, 166, 2093, 2202
BstMBI	GATC	9 cut(s)	6, 573, 948, 1139, 1417, 1629, 1908, 2230, 2263
BstMWI	GCNNNNNNNGC	6 cut(s)	408, 704, 713, 1743, 1788, 1865
BstNI	CCWGG	1 cut(s)	2114
BstNSI	RCATGY	1 cut(s)	138
BstSCI	CCNGG	3 cut(s)	27, 683, 2112
BstSLI	GKGCMC	2 cut(s)	709, 2076
BstUI	CGCG	1 cut(s)	827
BstV1I	GCAGC	5 cut(s)	240, 1253, 1519, 1845, 2169
BstV2I	GAAGAC	4 cut(s)	30, 1433, 2186, 2270
BstX2I	RGATCY	1 cut(s)	6
BstYI	RGATCY	1 cut(s)	6
BsuRI	GGCC	3 cut(s)	32, 707, 1490
BtsIMutI	CAGTG	1 cut(s)	1763
BveI	ACCTGC	2 cut(s)	174, 1773
Cac8I	GCNNGC	4 cut(s)	1005, 1015, 1186, 1512
CaiI	CAGNNNCTG	3 cut(s)	1013, 1354, 1643
CfoI	GCGC	2 cut(s)	718, 829
Cfr13I	GGNCC	7 cut(s)	30, 49, 705, 706, 1958, 2110, 2218
Csp6I	GTAC	8 cut(s)	187, 295, 753, 984, 1825, 1963, 2030, 2041
CviAII	CATG	4 cut(s)	135, 710, 776, 929
CviQI	GTAC	8 cut(s)	187, 295, 753, 984, 1825, 1963, 2030, 2041
DdeI	CTNAG	5 cut(s)	146, 542, 1332, 1829, 2015
DpnI	GATC	9 cut(s)	8, 575, 950, 1141, 1419, 1631, 1910, 2232, 2265
DpnII	GATC	9 cut(s)	6, 573, 948, 1139, 1417, 1629, 1908, 2230, 2263
DraI	TTTAAA	4 cut(s)	198, 1035, 1885, 1942
Eam1104I	CTCTTC	2 cut(s)	1610, 1845
EarI	CTCTTC	2 cut(s)	1610, 1845
Eco130I	CCWWGG	1 cut(s)	1912
Eco147I	AGGCCT	1 cut(s)	1490
Eco24I	GRGCYC	1 cut(s)	709
Eco31I	GGTCTC	2 cut(s)	166, 2093
Eco47I	GGWCC	4 cut(s)	49, 1958, 2110, 2218
Eco57I	CTGAAG	3 cut(s)	1469, 1770, 2018
Eco88I	CYCGRG	1 cut(s)	2205
EcoO109I	RGGNCCY	2 cut(s)	49, 705
EcoRII	CCWGG	1 cut(s)	2112
EcoT14I	CCWWGG	1 cut(s)	1912
EcoT22I	ATGCAT	1 cut(s)	1081
EcoT38I	GRGCYC	1 cut(s)	709
ErhI	CCWWGG	1 cut(s)	1912
FaeI	CATG	4 cut(s)	138, 713, 779, 932
FalI	AAGNNNNNCTT	2 cut(s)	1905, 1937
FaqI	GGGAC	1 cut(s)	531
FatI	CATG	4 cut(s)	134, 709, 775, 928
FauI	CCCGC	2 cut(s)	23, 376
FauNDI	CATATG	1 cut(s)	1651
FbaI	TGATCA	1 cut(s)	573
Fnu4HI	GCNGC	6 cut(s)	33, 229, 1242, 1508, 1834, 2183
FriOI	GRGCYC	1 cut(s)	709
Fsp4HI	GCNGC	6 cut(s)	33, 229, 1242, 1508, 1834, 2183
FspAI	RTGCGCAY	1 cut(s)	717
FspBI	CTAG	5 cut(s)	909, 1511, 1515, 1743, 2314
FspI	TGCGCA	1 cut(s)	717
GlaI	GCGC	2 cut(s)	717, 828
GluI	GCNGC	6 cut(s)	33, 229, 1242, 1508, 1834, 2183
GsuI	CTGGAG	2 cut(s)	147, 2162
HaeIII	GGCC	3 cut(s)	32, 707, 1490
HapII	CCGG	2 cut(s)	28, 684
HhaI	GCGC	2 cut(s)	718, 829
Hin1I	GRCGYC	1 cut(s)	1536
Hin1II	CATG	4 cut(s)	138, 713, 779, 932
Hin6I	GCGC	2 cut(s)	716, 827
HinP1I	GCGC	2 cut(s)	716, 827
HindIII	AAGCTT	4 cut(s)	819, 965, 1268, 1334
HinfI	GANTC	7 cut(s)	387, 392, 613, 1438, 1469, 1480, 1640
HpaII	CCGG	2 cut(s)	28, 684
HphI	GGTGA	6 cut(s)	47, 493, 1624, 1735, 2018, 2273
Hpy166II	GTNNAC	7 cut(s)	140, 152, 753, 984, 1756, 1965, 2074
Hpy188I	TCNGA	7 cut(s)	81, 378, 397, 618, 1503, 1750, 2016
Hpy8I	GTNNAC	7 cut(s)	140, 152, 753, 984, 1756, 1965, 2074
Hpy99I	CGWCG	3 cut(s)	368, 956, 1538
HpyCH4III	ACNGT	4 cut(s)	661, 1350, 1962, 2222
HpyCH4IV	ACGT	2 cut(s)	972, 1536
HpyF10VI	GCNNNNNNNGC	6 cut(s)	408, 704, 713, 1743, 1788, 1865
HpyF3I	CTNAG	5 cut(s)	146, 542, 1332, 1829, 2015
HpySE526I	ACGT	2 cut(s)	972, 1536
Hsp92I	GRCGYC	1 cut(s)	1536
Hsp92II	CATG	4 cut(s)	138, 713, 779, 932
HspAI	GCGC	2 cut(s)	716, 827
Ksp22I	TGATCA	1 cut(s)	573
Kzo9I	GATC	9 cut(s)	6, 573, 948, 1139, 1417, 1629, 1908, 2230, 2263
LmnI	GCTCC	2 cut(s)	67, 1456
Lsp1109I	GCAGC	5 cut(s)	240, 1253, 1519, 1845, 2169
MaeI	CTAG	5 cut(s)	909, 1511, 1515, 1743, 2314
MaeII	ACGT	2 cut(s)	972, 1536
MaeIII	GTNAC	7 cut(s)	275, 661, 1021, 1291, 1645, 2053, 2295
MalI	GATC	9 cut(s)	8, 575, 950, 1141, 1419, 1631, 1910, 2232, 2265
MboI	GATC	9 cut(s)	6, 573, 948, 1139, 1417, 1629, 1908, 2230, 2263
MfeI	CAATTG	2 cut(s)	1460, 1619
MflI	RGATCY	1 cut(s)	6
MhlI	GDGCHC	3 cut(s)	373, 709, 2076
MlyI	GAGTC	2 cut(s)	1447, 1478
MmeI	TCCRAC	1 cut(s)	66
Mph1103I	ATGCAT	1 cut(s)	1081
MroXI	GAANNNNTTC	1 cut(s)	428
MslI	CAYNNNNRTG	1 cut(s)	2159
MspI	CCGG	2 cut(s)	28, 684
MspR9I	CCNGG	3 cut(s)	29, 685, 2114
MssI	GTTTAAAC	1 cut(s)	198
MunI	CAATTG	2 cut(s)	1460, 1619
Mva1269I	GAATGC	1 cut(s)	212
MvaI	CCWGG	1 cut(s)	2114
MvnI	CGCG	1 cut(s)	827
MwoI	GCNNNNNNNGC	6 cut(s)	408, 704, 713, 1743, 1788, 1865
NciI	CCSGG	2 cut(s)	29, 685
NdeI	CATATG	1 cut(s)	1651
NdeII	GATC	9 cut(s)	6, 573, 948, 1139, 1417, 1629, 1908, 2230, 2263
NheI	GCTAGC	1 cut(s)	1510
NlaIII	CATG	4 cut(s)	138, 713, 779, 932
NlaIV	GGNNCC	3 cut(s)	50, 707, 1071
NmuCI	GTSAC	1 cut(s)	2053
NsbI	TGCGCA	1 cut(s)	717
NsiI	ATGCAT	1 cut(s)	1081
NspI	RCATGY	1 cut(s)	138
NspV	TTCGAA	2 cut(s)	390, 1160
PceI	AGGCCT	1 cut(s)	1490
PciI	ACATGT	1 cut(s)	134
PcsI	WCGNNNNNNNCGW	1 cut(s)	608
PctI	GAATGC	1 cut(s)	212
PdmI	GAANNNNTTC	1 cut(s)	428
PfeI	GAWTC	5 cut(s)	387, 392, 613, 1480, 1640
PflFI	GACNNNGTC	1 cut(s)	1774
PkrI	GCNGC	6 cut(s)	34, 230, 1243, 1509, 1835, 2184
PleI	GAGTC	2 cut(s)	1446, 1477
PmeI	GTTTAAAC	1 cut(s)	198
PpsI	GAGTC	2 cut(s)	1446, 1477
PpuMI	RGGWCCY	1 cut(s)	49
PscI	ACATGT	1 cut(s)	134
PshBI	ATTAAT	2 cut(s)	329, 2159
Psp5II	RGGWCCY	1 cut(s)	49
Psp6I	CCWGG	1 cut(s)	2112
PspGI	CCWGG	1 cut(s)	2112
PspN4I	GGNNCC	3 cut(s)	50, 707, 1071
PspOMI	GGGCCC	1 cut(s)	705
PspPI	GGNCC	7 cut(s)	30, 49, 705, 706, 1958, 2110, 2218
PspPPI	RGGWCCY	1 cut(s)	49
PstNI	CAGNNNCTG	3 cut(s)	1013, 1354, 1643
PsuI	RGATCY	1 cut(s)	6
PsyI	GACNNNGTC	1 cut(s)	1774
RsaI	GTAC	8 cut(s)	188, 296, 754, 985, 1826, 1964, 2031, 2042
RsaNI	GTAC	8 cut(s)	187, 295, 753, 984, 1825, 1963, 2030, 2041
RseI	CAYNNNNRTG	1 cut(s)	2159
SatI	GCNGC	6 cut(s)	33, 229, 1242, 1508, 1834, 2183
Sau3AI	GATC	9 cut(s)	6, 573, 948, 1139, 1417, 1629, 1908, 2230, 2263
Sau96I	GGNCC	7 cut(s)	30, 49, 705, 706, 1958, 2110, 2218
SchI	GAGTC	2 cut(s)	1447, 1478
ScrFI	CCNGG	3 cut(s)	29, 685, 2114
SduI	GDGCHC	3 cut(s)	373, 709, 2076
SfuI	TTCGAA	2 cut(s)	390, 1160
SinI	GGWCC	4 cut(s)	49, 1958, 2110, 2218
SmiMI	CAYNNNNRTG	1 cut(s)	2159
SmlI	CTYRAG	5 cut(s)	169, 1042, 1337, 1407, 2186
SmoI	CTYRAG	5 cut(s)	169, 1042, 1337, 1407, 2186
SseBI	AGGCCT	1 cut(s)	1490
SsiI	CCGC	5 cut(s)	16, 33, 383, 402, 464
SspMI	CTAG	5 cut(s)	909, 1511, 1515, 1743, 2314
StuI	AGGCCT	1 cut(s)	1490
StyD4I	CCNGG	3 cut(s)	27, 683, 2112
StyI	CCWWGG	1 cut(s)	1912
TaaI	ACNGT	4 cut(s)	661, 1350, 1962, 2222
TaiI	ACGT	2 cut(s)	975, 1539
TaqI	TCGA	6 cut(s)	390, 432, 729, 951, 1138, 1160
TatI	WGTACW	5 cut(s)	294, 752, 983, 2029, 2040
TauI	GCSGC	1 cut(s)	35
TfiI	GAWTC	5 cut(s)	387, 392, 613, 1480, 1640
TscAI	CASTG	1 cut(s)	1770
TseFI	GTSAC	1 cut(s)	2053
TseI	GCWGC	5 cut(s)	228, 1241, 1507, 1833, 2182
Tsp45I	GTSAC	1 cut(s)	2053
TspDTI	ATGAA	7 cut(s)	315, 437, 752, 831, 1146, 1377, 2143
TspGWI	ACGGA	1 cut(s)	1040
TspRI	CASTG	1 cut(s)	1770
Tth111I	GACNNNGTC	1 cut(s)	1774
VneI	GTGCAC	1 cut(s)	2072
VpaK11BI	GGWCC	4 cut(s)	49, 1958, 2110, 2218
VspI	ATTAAT	2 cut(s)	329, 2159
XapI	RAATTY	6 cut(s)	320, 358, 1155, 1879, 2104, 2251
XceI	RCATGY	1 cut(s)	138
XmnI	GAANNNNTTC	1 cut(s)	428
XspI	CTAG	5 cut(s)	909, 1511, 1515, 1743, 2314
ZraI	GACGTC	1 cut(s)	1537
Zsp2I	ATGCAT	1 cut(s)	1081

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.