Rw5G014570

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016134
Physical Location & Seq
Forward (+)
17508423 .. 17510761
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G014570.1

Sequence Viewer

Length: 2310 bp
ATGAAACCCCTCCTCCGCCCCATCCACTACTTCATTCCCAAACCCCCATGGCGGCGCCGCTACTCCCACACCCAACCAGACCCATCTCCCACCGCCCATGACCTCCTCACCATCCTCAACAAATCCGACGGCGCTGAATCCCTGATCTCACACCTCTCCAATAACAAGCTCATCTCTCTGATCCAAGAACACCACACAAACCCACAATTGGGCTTTCGCCTCTTCATCTGGGCCACCCAAAGATCCAAGCTTTGCACCCGAATGTGTCGTTCCACAGTGATCGACATGCTCCTGGGGGACGATAATGGTGTTGAACTCTACTGGAGGACTCTGGAGGAGTTCAGAGATTGCGGGATTCGAATCGGGTGTGATGCATTTTCTGTGCTGATCAGAGGGTATGATAAGCTGGGCATTGCCGAGAAGGCGGTGGAGGCTTTTTCAAGGATGAAAGATTTCGATTGTAAGCCTGATGTTTATACTTACAATGCGGTTTTATATGTTTTGGTGAGGAAGGAAGTGTTTTTGTTAGCTTTAGCTGTGTATAATCAAATGTTGAAGTGTAATCTTAGTCCCAGTAGGACTACTTATAGCATTTTGATCAATGGGTTTTGCAAGAGTAGGAAAACCCAGGATGCGATTCAGATGTTTGATGAGATGACACAGAGAGGGATTGCACCCAATACCGTTACTTATACTATTATCATTTCCGGGTTGTGCAAGGCAAAGAGGGCTCATGATGCGCATAGGCTGGTCGATACGATGAAGGAAACCGGGTGTACTCCTGATGTGATTACTTACCATGCTTTGCTTGATGGGTATTGTAAGTTAGGTAGGCTTGACGAAGCTTACGCACTCGTGAGGTTATTGGAGAGGGATGGTTATGTTCTTGGAGTGGAGGGATATAGTTCCTTGATTTTGGGTTTGTTTAGAGCTAGGAGGTTTGATGAGGCACATGAGTTGTATGGGAAGTTGATCGGCGAGGGGATTAAGCTTGATGTTATCTTGTGTACTATATTGATTAAGGGGCTGGCAGACGCTGGCAGGGTTACGGATGCTTTAAACTTCTTGAGTGAGATGAGTAAGAAAGGTTTGGTTCCAGATGCATACTGCTATAATGCTGTCATCAAAGGGTTCTGCGATTTAGGTCTTTTGGATGAAGCTCGATCTCTCCATCTTGAGATTTCGAAGCAAGATTGCTTCCCTAATGCCTGCACTTACACCATTCTCATTTGTGGTATCACAGATTTCAATGAGATGGAGAAGCTTGGATGTGTTCCTTCTGTTGTAACCTTCAATGCTCTTATTGATGGGCTTTGTAAGGCTTCCAAGCTTGAGGATGCACAGTTGCTGTTTTACAAGATGGAGATAGGAAAAAAACCTTCATTATTTCTTCGTCTTTCTCAAGGTAGTGATCGGGTTATTGATAGTGCAAGTCTTCAAAAGAAGGTGGAGCAATTGTGCGAGTCAGGATTGATTCTTCAGGCCTATAAACTTCTGATGCAGCTAGCTAGTAGTGGGGTTGCCCCCGACGTCATCACTTACAACACTCTAATCAATGGATTTTGCAAAAATGGAAACATAGATGGTGCTTTCAAACTCTTCAAGGATATGCAATTGAAAGGGATCACCCCAGATTCTGTTACATATGGAACACTTATAGATGGACTTCAAAGGGTTGAAAGAGAAGAAGATGCCTTTGTGGTCTTTAATCAAATGGTGAAGAATGGATGCACACCTAGCTCTGAAGTTTACAAATCACTGATGACTTGGTCTTGCAGGTATAGCAAGGTTTCGGTAGCTGTTAGTCTTTGGTTGAAGTACCTGAGCAGCCTTCCTCACAGAGAAGAGGAAGCAATCAAAGCAATAGAGGAAAATTTTAAAGAGGGGCAAATTGAGAAGGCGATCCAAGGCTTACTTGAAATGGATGTAAAGTTTAAAGATTTAGATTTAGGACCGTACACTATTTTGCTCATTGGTTTGTGTCAGGTGCAAAGAGTGGGTGAAGCTCTGAGAATGTTCTCTGTACTTCAGGAGTACAAAGTCAATGTCACTCCACCAAGTTGTGTGCACTTGATAAATGGTCTCTGCAAAGAAGGGAATTTGGACCTGGCAATAAATGTTTTCCATTATACTCTGGAGAGGGGTTTCATATTAATGCCTGAAATATGCAATAAGCTGCTCAAGTGTCTTCTTCGTTCCCGAGACAAAAAGGACCGTGCCTTTGATCTCATTCGTAGGATGAGAAATTTTGGATATGATCTTGATGCTTGTCTTCACCAGACTACAAAGTTACTTCTACAATTTCACTAG

Protein Analysis

769

Amino Acids

86.95

Weight (kDa)

8.1

Isoelectric Point (pI)

33.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 126 - 168 3.5e-07 PPR repeat family
PPR_2 PF13041 157 - 205 1.7e-12 PPR repeat family
TPR_24 PF23276 164 - 282 1.6e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 176 - 287 1.1e-12 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 188 - 220 3.1e-10 PPR repeat
PPR_2 PF13041 195 - 240 3.7e-18 PPR repeat family
PPR PF01535 195 - 224 5.5e-08 PPR repeat
PPR_3 PF13812 215 - 273 8.3e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 222 - 255 2.4e-11 PPR repeat
PPR_2 PF13041 226 - 275 2.2e-17 PPR repeat family
PPR_1 PF12854 258 - 287 4.9e-11 PPR repeat
PPR_3 PF13812 327 - 378 6.9e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 332 - 380 3.6e-13 PPR repeat family
PPR_1 PF12854 362 - 389 1.9e-07 PPR repeat
PPR_2 PF13041 366 - 412 2.9e-11 PPR repeat family
PPR_2 PF13041 416 - 440 7.2e-07 PPR repeat family
PPR_1 PF12854 423 - 455 2.3e-11 PPR repeat
PPR_2 PF13041 426 - 455 6.9e-06 PPR repeat family
PPR PF01535 429 - 455 9.3e-06 PPR repeat
PPR_2 PF13041 486 - 523 7.4e-09 PPR repeat family
PPR_long PF17177 492 - 627 1.3e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 498 - 553 3.2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 506 - 538 1.4e-16 PPR repeat
PPR_2 PF13041 509 - 556 4.7e-21 PPR repeat family
PPR PF01535 512 - 542 8.9e-11 PPR repeat
PPR_3 PF13812 532 - 588 7.1e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 540 - 573 2.3e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016134)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79540
fragaria_vesca FvH4_3g13661
malus_domestica MD05G1241300.v1.1
prunus_persica Prupe.4G124200_v2.0.a1 Prupe.4G124200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0022901
rosa_laevigata RLG00000032681
rosa_multiflora Rmu_co8399345.1_g000001
rosa_roxburghii Rroxscaffold_1G00056430
rosa_rugosa Rorug05G0075400
rosa_samantha Rh5AG161900 Rh5BG161100 Rh5CG175600 Rh5DG162300
rosa_wichuraiana Rw5G014570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1533
Acc16I TGCGCA 1 cut(s) 741
Acc36I ACCTGC 1 cut(s) 1767
AccB1I GGYRCC 1 cut(s) 54
AciI CCGC 7 cut(s) 16, 52, 58, 93, 351, 425, 488
AclWI GGATC 4 cut(s) 175, 237, 1631, 1897
AcsI RAATTY 3 cut(s) 1873, 2098, 2245
AcuI CTGAAG 3 cut(s) 1463, 1764, 2012
AcyI GRCGYC 2 cut(s) 55, 1530
AfaI GTAC 6 cut(s) 778, 1009, 1820, 1958, 2025, 2036
AfiI CCNNNNNNNGG 3 cut(s) 51, 208, 209
AjnI CCWGG 3 cut(s) 291, 627, 2106
Alw21I GWGCWC 1 cut(s) 2070
Alw26I GTCTC 2 cut(s) 2087, 2196
Alw44I GTGCAC 1 cut(s) 2066
AlwI GGATC 4 cut(s) 175, 237, 1631, 1897
AlwNI CAGNNNCTG 3 cut(s) 1037, 1348, 1637
Ama87I CYCGRG 1 cut(s) 2199
AoxI GGCC 2 cut(s) 231, 1482
ApaLI GTGCAC 1 cut(s) 2066
ApeKI GCWGC 3 cut(s) 1501, 1827, 2176
ApoI RAATTY 3 cut(s) 1873, 2098, 2245
ArsI GACNNNNNNTTYG 4 cut(s) 2031, 2063, 2204, 2236
AseI ATTAAT 1 cut(s) 2153
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 3 cut(s) 57, 134, 742
AspS9I GGNCC 4 cut(s) 231, 1952, 2104, 2212
AsuC2I CCSGG 2 cut(s) 709, 772
AsuHPI GGTGA 6 cut(s) 100, 517, 1618, 1729, 2012, 2267
AsuII TTCGAA 2 cut(s) 358, 1184
AsuNHI GCTAGC 1 cut(s) 1504
AvaI CYCGRG 1 cut(s) 2199
AvaII GGWCC 3 cut(s) 1952, 2104, 2212
BaeGI GKGCMC 1 cut(s) 2070
BanI GGYRCC 1 cut(s) 54
BanII GRGCYC 1 cut(s) 733
BauI CACGAG 1 cut(s) 854
BbsI GAAGAC 3 cut(s) 1427, 2180, 2264
Bbv12I GWGCWC 1 cut(s) 2070
BbvI GCAGC 3 cut(s) 1513, 1839, 2163
BceAI ACGGC 1 cut(s) 145
BciT130I CCWGG 3 cut(s) 293, 629, 2108
BclI TGATCA 2 cut(s) 387, 597
BcnI CCSGG 2 cut(s) 709, 772
BcoDI GTCTC 2 cut(s) 2087, 2196
BfaI CTAG 5 cut(s) 933, 1505, 1509, 1737, 2308
BfoI RGCGCY 2 cut(s) 58, 135
BfuAI ACCTGC 1 cut(s) 1767
BglI GCCNNNNNGGC 1 cut(s) 422
BisI GCNGC 5 cut(s) 53, 58, 1502, 1828, 2177
BlsI GCNGC 5 cut(s) 54, 59, 1503, 1829, 2178
Bme1390I CCNGG 5 cut(s) 293, 629, 709, 772, 2108
Bme18I GGWCC 3 cut(s) 1952, 2104, 2212
BmeT110I CYCGRG 1 cut(s) 2199
BmgT120I GGNCC 4 cut(s) 231, 1952, 2104, 2212
BmiI GGNNCC 2 cut(s) 56, 1095
BmrFI CCNGG 5 cut(s) 293, 629, 709, 772, 2108
BmrI ACTGGG 1 cut(s) 567
BmtI GCTAGC 1 cut(s) 1508
BmuI ACTGGG 1 cut(s) 567
BpiI GAAGAC 3 cut(s) 1427, 2180, 2264
BpmI CTGGAG 3 cut(s) 343, 353, 2156
Bpu10I CCTNAGC 1 cut(s) 1823
Bpu14I TTCGAA 2 cut(s) 358, 1184
BpuEI CTTGAG 5 cut(s) 1087, 1196, 1352, 1386, 2165
BpuMI CCSGG 2 cut(s) 709, 772
BsaBI GATNNNNATC 1 cut(s) 359
BsaHI GRCGYC 2 cut(s) 55, 1530
BsaI GGTCTC 1 cut(s) 2087
BsaJI CCNNGG 4 cut(s) 47, 292, 627, 1906
BsaXI ACNNNNNCTCC 3 cut(s) 27, 1442, 1472
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 208, 209
Bse1I ACTGG 2 cut(s) 326, 573
Bse3DI GCAATG 1 cut(s) 411
Bse8I GATNNNNATC 1 cut(s) 359
BseBI CCWGG 3 cut(s) 293, 629, 2108
BseDI CCNNGG 4 cut(s) 47, 292, 627, 1906
BseJI GATNNNNATC 1 cut(s) 359
BseLI CCNNNNNNNGG 3 cut(s) 51, 208, 209
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 2 cut(s) 1814, 2000
BseNI ACTGG 2 cut(s) 326, 573
BseRI GAGGAG 2 cut(s) 95, 350
BseSI GKGCMC 1 cut(s) 2070
BseXI GCAGC 3 cut(s) 1513, 1839, 2163
BseYI CCCAGC 1 cut(s) 406
BsgI GTGCAG 1 cut(s) 1195
BshFI GGCC 2 cut(s) 233, 1484
BshNI GGYRCC 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 2070
BsiHKCI CYCGRG 1 cut(s) 2199
BsiSI CCGG 2 cut(s) 708, 771
BslFI GGGAC 2 cut(s) 311, 555
BslI CCNNNNNNNGG 3 cut(s) 51, 208, 209
BsmAI GTCTC 2 cut(s) 2087, 2196
BsmFI GGGAC 2 cut(s) 311, 555
BsnI GGCC 2 cut(s) 233, 1484
Bso31I GGTCTC 1 cut(s) 2087
BsoBI CYCGRG 1 cut(s) 2199
Bsp119I TTCGAA 2 cut(s) 358, 1184
Bsp1286I GDGCHC 2 cut(s) 733, 2070
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 7 cut(s) 16, 52, 58, 93, 351, 425, 488
BspANI GGCC 2 cut(s) 233, 1484
BspCNI CTCAG 2 cut(s) 1815, 2001
BspHI TCATGA 1 cut(s) 733
BspLI GGNNCC 2 cut(s) 56, 1095
BspMI ACCTGC 1 cut(s) 1767
BspOI GCTAGC 1 cut(s) 1508
BspPI GGATC 4 cut(s) 175, 237, 1631, 1897
BspT104I TTCGAA 2 cut(s) 358, 1184
BspT107I GGYRCC 1 cut(s) 54
BspTNI GGTCTC 1 cut(s) 2087
BsrDI GCAATG 1 cut(s) 411
BsrI ACTGG 2 cut(s) 326, 573
BssECI CCNNGG 4 cut(s) 47, 292, 627, 1906
BssNI GRCGYC 2 cut(s) 55, 1530
BssSI CACGAG 1 cut(s) 854
BssT1I CCWWGG 2 cut(s) 47, 1906
Bst2BI CACGAG 1 cut(s) 854
Bst2UI CCWGG 3 cut(s) 293, 629, 2108
Bst4CI ACNGT 5 cut(s) 277, 685, 1344, 1956, 2216
Bst6I CTCTTC 3 cut(s) 227, 1604, 1839
BstACI GRCGYC 2 cut(s) 55, 1530
BstBI TTCGAA 2 cut(s) 358, 1184
BstC8I GCNNGC 4 cut(s) 1029, 1039, 1210, 1506
BstDEI CTNAG 3 cut(s) 566, 1823, 2009
BstDSI CCRYGG 1 cut(s) 47
BstH2I RGCGCY 2 cut(s) 58, 135
BstHHI GCGC 3 cut(s) 57, 134, 742
BstMAI GTCTC 2 cut(s) 2087, 2196
BstMWI GCNNNNNNNGC 7 cut(s) 422, 431, 728, 737, 1737, 1782, 1859
BstNI CCWGG 3 cut(s) 293, 629, 2108
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 5 cut(s) 291, 627, 707, 770, 2106
BstSLI GKGCMC 1 cut(s) 2070
BstV1I GCAGC 3 cut(s) 1513, 1839, 2163
BstV2I GAAGAC 3 cut(s) 1427, 2180, 2264
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BsuRI GGCC 2 cut(s) 233, 1484
BtgI CCRYGG 1 cut(s) 47
BtsIMutI CAGTG 2 cut(s) 282, 1757
BveI ACCTGC 1 cut(s) 1767
Cac8I GCNNGC 4 cut(s) 1029, 1039, 1210, 1506
CaiI CAGNNNCTG 3 cut(s) 1037, 1348, 1637
CciI TCATGA 1 cut(s) 733
CfoI GCGC 3 cut(s) 57, 134, 742
Cfr13I GGNCC 4 cut(s) 231, 1952, 2104, 2212
CseI GACGC 1 cut(s) 1043
Csp6I GTAC 6 cut(s) 777, 1008, 1819, 1957, 2024, 2035
CviAII CATG 6 cut(s) 48, 98, 286, 734, 800, 953
CviQI GTAC 6 cut(s) 777, 1008, 1819, 1957, 2024, 2035
DdeI CTNAG 3 cut(s) 566, 1823, 2009
DinI GGCGCC 1 cut(s) 56
DraI TTTAAA 3 cut(s) 1059, 1879, 1936
Eam1104I CTCTTC 3 cut(s) 227, 1604, 1839
EarI CTCTTC 3 cut(s) 227, 1604, 1839
EciI GGCGGA 1 cut(s) 5
Eco130I CCWWGG 2 cut(s) 47, 1906
Eco147I AGGCCT 1 cut(s) 1484
Eco24I GRGCYC 1 cut(s) 733
Eco31I GGTCTC 1 cut(s) 2087
Eco47I GGWCC 3 cut(s) 1952, 2104, 2212
Eco57I CTGAAG 3 cut(s) 1463, 1764, 2012
Eco88I CYCGRG 1 cut(s) 2199
EcoRII CCWGG 3 cut(s) 291, 627, 2106
EcoT14I CCWWGG 2 cut(s) 47, 1906
EcoT22I ATGCAT 2 cut(s) 376, 1105
EcoT38I GRGCYC 1 cut(s) 733
EgeI GGCGCC 1 cut(s) 56
EheI GGCGCC 1 cut(s) 56
ErhI CCWWGG 2 cut(s) 47, 1906
FaeI CATG 6 cut(s) 51, 101, 289, 737, 803, 956
FalI AAGNNNNNCTT 2 cut(s) 1899, 1931
FaqI GGGAC 2 cut(s) 311, 555
FatI CATG 6 cut(s) 47, 97, 285, 733, 799, 952
FauI CCCGC 1 cut(s) 344
FauNDI CATATG 1 cut(s) 1645
FbaI TGATCA 2 cut(s) 387, 597
Fnu4HI GCNGC 5 cut(s) 53, 58, 1502, 1828, 2177
FriOI GRGCYC 1 cut(s) 733
Fsp4HI GCNGC 5 cut(s) 53, 58, 1502, 1828, 2177
FspAI RTGCGCAY 1 cut(s) 741
FspBI CTAG 5 cut(s) 933, 1505, 1509, 1737, 2308
FspI TGCGCA 1 cut(s) 741
GlaI GCGC 3 cut(s) 56, 133, 741
GluI GCNGC 5 cut(s) 53, 58, 1502, 1828, 2177
GsaI CCCAGC 1 cut(s) 410
GsuI CTGGAG 3 cut(s) 343, 353, 2156
HaeII RGCGCY 2 cut(s) 58, 135
HaeIII GGCC 2 cut(s) 233, 1484
HapII CCGG 2 cut(s) 708, 771
HgaI GACGC 1 cut(s) 1043
HhaI GCGC 3 cut(s) 57, 134, 742
Hin1I GRCGYC 2 cut(s) 55, 1530
Hin1II CATG 6 cut(s) 51, 101, 289, 737, 803, 956
Hin6I GCGC 3 cut(s) 55, 132, 740
HinP1I GCGC 3 cut(s) 55, 132, 740
HindIII AAGCTT 5 cut(s) 248, 843, 989, 1262, 1328
HinfI GANTC 8 cut(s) 137, 328, 355, 360, 637, 1463, 1474, 1634
HpaII CCGG 2 cut(s) 708, 771
HphI GGTGA 6 cut(s) 100, 517, 1618, 1729, 2012, 2267
Hpy166II GTNNAC 5 cut(s) 777, 1008, 1750, 1959, 2068
Hpy188I TCNGA 8 cut(s) 127, 180, 344, 392, 642, 1497, 1744, 2010
Hpy8I GTNNAC 5 cut(s) 777, 1008, 1750, 1959, 2068
Hpy99I CGWCG 2 cut(s) 131, 1532
HpyCH4III ACNGT 5 cut(s) 277, 685, 1344, 1956, 2216
HpyCH4IV ACGT 1 cut(s) 1530
HpyF10VI GCNNNNNNNGC 7 cut(s) 422, 431, 728, 737, 1737, 1782, 1859
HpyF3I CTNAG 3 cut(s) 566, 1823, 2009
HpySE526I ACGT 1 cut(s) 1530
Hsp92I GRCGYC 2 cut(s) 55, 1530
Hsp92II CATG 6 cut(s) 51, 101, 289, 737, 803, 956
HspAI GCGC 3 cut(s) 55, 132, 740
KasI GGCGCC 1 cut(s) 54
Ksp22I TGATCA 2 cut(s) 387, 597
LmnI GCTCC 2 cut(s) 294, 1450
Lsp1109I GCAGC 3 cut(s) 1513, 1839, 2163
MaeI CTAG 5 cut(s) 933, 1505, 1509, 1737, 2308
MaeII ACGT 1 cut(s) 1530
MaeIII GTNAC 6 cut(s) 685, 1045, 1285, 1639, 2047, 2289
MfeI CAATTG 3 cut(s) 206, 1454, 1613
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 2 cut(s) 733, 2070
MluCI AATT 8 cut(s) 206, 1454, 1613, 1873, 1890, 2098, 2245, 2300
Mly113I GGCGCC 1 cut(s) 55
MlyI GAGTC 2 cut(s) 322, 1472
MmeI TCCRAC 1 cut(s) 150
Mph1103I ATGCAT 2 cut(s) 376, 1105
MroXI GAANNNNTTC 1 cut(s) 452
MseI TTAA 7 cut(s) 987, 1020, 1058, 1707, 1878, 1935, 2153
MslI CAYNNNNRTG 2 cut(s) 260, 2153
MspI CCGG 2 cut(s) 708, 771
MspR9I CCNGG 5 cut(s) 293, 629, 709, 772, 2108
MunI CAATTG 3 cut(s) 206, 1454, 1613
MvaI CCWGG 3 cut(s) 293, 629, 2108
MwoI GCNNNNNNNGC 7 cut(s) 422, 431, 728, 737, 1737, 1782, 1859
NarI GGCGCC 1 cut(s) 55
NciI CCSGG 2 cut(s) 709, 772
NcoI CCATGG 1 cut(s) 47
NdeI CATATG 1 cut(s) 1645
NheI GCTAGC 1 cut(s) 1504
NlaIII CATG 6 cut(s) 51, 101, 289, 737, 803, 956
NlaIV GGNNCC 2 cut(s) 56, 1095
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 2047
NsbI TGCGCA 1 cut(s) 741
NsiI ATGCAT 2 cut(s) 376, 1105
NspI RCATGY 1 cut(s) 289
NspV TTCGAA 2 cut(s) 358, 1184
PagI TCATGA 1 cut(s) 733
PceI AGGCCT 1 cut(s) 1484
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 6 cut(s) 137, 355, 360, 637, 1474, 1634
PflFI GACNNNGTC 1 cut(s) 1768
PkrI GCNGC 5 cut(s) 54, 59, 1503, 1829, 2178
PleI GAGTC 2 cut(s) 322, 1471
PluTI GGCGCC 1 cut(s) 58
PpsI GAGTC 2 cut(s) 322, 1471
PshBI ATTAAT 1 cut(s) 2153
Psp6I CCWGG 3 cut(s) 291, 627, 2106
PspFI CCCAGC 1 cut(s) 406
PspGI CCWGG 3 cut(s) 291, 627, 2106
PspN4I GGNNCC 2 cut(s) 56, 1095
PspPI GGNCC 4 cut(s) 231, 1952, 2104, 2212
PstNI CAGNNNCTG 3 cut(s) 1037, 1348, 1637
PsuI RGATCY 1 cut(s) 242
PsyI GACNNNGTC 1 cut(s) 1768
RsaI GTAC 6 cut(s) 778, 1009, 1820, 1958, 2025, 2036
RsaNI GTAC 6 cut(s) 777, 1008, 1819, 1957, 2024, 2035
RseI CAYNNNNRTG 2 cut(s) 260, 2153
SaqAI TTAA 7 cut(s) 987, 1020, 1058, 1707, 1878, 1935, 2153
SatI GCNGC 5 cut(s) 53, 58, 1502, 1828, 2177
Sau96I GGNCC 4 cut(s) 231, 1952, 2104, 2212
SchI GAGTC 2 cut(s) 322, 1472
ScrFI CCNGG 5 cut(s) 293, 629, 709, 772, 2108
SduI GDGCHC 2 cut(s) 733, 2070
SfoI GGCGCC 1 cut(s) 56
SfuI TTCGAA 2 cut(s) 358, 1184
SinI GGWCC 3 cut(s) 1952, 2104, 2212
SmiMI CAYNNNNRTG 2 cut(s) 260, 2153
SmlI CTYRAG 5 cut(s) 1066, 1175, 1331, 1401, 2180
SmoI CTYRAG 5 cut(s) 1066, 1175, 1331, 1401, 2180
Sse9I AATT 8 cut(s) 206, 1454, 1613, 1873, 1890, 2098, 2245, 2300
SseBI AGGCCT 1 cut(s) 1484
SsiI CCGC 7 cut(s) 16, 52, 58, 93, 351, 425, 488
SspDI GGCGCC 1 cut(s) 54
SspMI CTAG 5 cut(s) 933, 1505, 1509, 1737, 2308
StuI AGGCCT 1 cut(s) 1484
StyD4I CCNGG 5 cut(s) 291, 627, 707, 770, 2106
StyI CCWWGG 2 cut(s) 47, 1906
TaaI ACNGT 5 cut(s) 277, 685, 1344, 1956, 2216
TaiI ACGT 1 cut(s) 1533
TaqI TCGA 6 cut(s) 282, 358, 456, 753, 1162, 1184
TasI AATT 8 cut(s) 206, 1454, 1613, 1873, 1890, 2098, 2245, 2300
TatI WGTACW 4 cut(s) 776, 1007, 2023, 2034
TauI GCSGC 2 cut(s) 55, 60
TfiI GAWTC 6 cut(s) 137, 355, 360, 637, 1474, 1634
Tru1I TTAA 7 cut(s) 987, 1020, 1058, 1707, 1878, 1935, 2153
Tru9I TTAA 7 cut(s) 987, 1020, 1058, 1707, 1878, 1935, 2153
TscAI CASTG 2 cut(s) 282, 1764
TseFI GTSAC 1 cut(s) 2047
TseI GCWGC 3 cut(s) 1501, 1827, 2176
Tsp45I GTSAC 1 cut(s) 2047
TspDTI ATGAA 8 cut(s) 17, 22, 214, 461, 776, 1170, 1371, 2137
TspGWI ACGGA 1 cut(s) 1064
TspRI CASTG 2 cut(s) 282, 1764
Tth111I GACNNNGTC 1 cut(s) 1768
VneI GTGCAC 1 cut(s) 2066
VpaK11BI GGWCC 3 cut(s) 1952, 2104, 2212
VspI ATTAAT 1 cut(s) 2153
XapI RAATTY 3 cut(s) 1873, 2098, 2245
XceI RCATGY 1 cut(s) 289
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 5 cut(s) 933, 1505, 1509, 1737, 2308
ZraI GACGTC 1 cut(s) 1531
Zsp2I ATGCAT 2 cut(s) 376, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.