Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0011204

Physical Location & Seq

Genomic Coordinates Reverse (-)

15465188 .. 15466105

Gene Structure 918 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g22400.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 918 bp

ATGTCTATCTTCACTCAAACCAAGCTCCTCCCAAGCCCCAAGCACCATGTTCACACACTATGCCACCCTCCAGTCCTCCGCACGACTGTCCATTGCTCCATTACCACCACAAAGCCAAAGCCAGTCCCCGAAAAGCTCGTCATCGAACCGCCCTGGCTGCCGCAGCTGGAACCCTCACCCGACATCGCCCGGCCGATGCTCAATGACCCGAGTCTACTATCAACATGGTCACACCGTGCATGGGTAGCGAGCGGGTGCACCACTGTGCTCATTTCTCTAGCTAAATCGGTAGTGGGTGCAGCCAATTCACACATATGGGCCGACCCCATATTAGCGGGCTTGGTCGGGTATGTGTTGGCGGATCTCGGGTCCGGAGTTTACCATTGGGGAATTGACAACTATGGTGGTGCCTCTACTCCAATTTTCGGTGCACAAATAGAAGCATTTCAAGGCCACCATAAGTGGCCATGGACTATCACTAAGCGCCAGTTTGCTAACAACTTACATGCCCTGGCTCGTGTCATTACTTTCACAGTGCTTCCCTTAGACCTTGCTTTTGATGATCCTATTCTTCATGGCTTTGTTGCTGTTTGCTCTGGTTGCATTATGTTTAGTCAACAATTTCATGCTTGGGCTCACGGCACAAAGAGCCGGCTACCACCATTGGTTGTGGCATTGCAAGATTCTGGCATACTTATATCTCGATCGCAACATTCGGCGCACCACCGGCAACCTTACAACAACAACTACTGCATTGTGAGTGGAGTTTGGAACGAGTTTTTGGATAAGCAGAAGGTATTTGAAGCTTTGGAGATGATCTTGTTTTTTAAGCAAGGGTTAAGACCTAGGTCTTGGAGTGAACCTAGCTCTGAGTGGATTGAAGAGGCTGAGACGATTTCTGAACTTGCAGACAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009941 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016053 GO:0016491 GO:0016567 GO:0016705 GO:0016740 GO:0019538 GO:0019637 GO:0019752 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030433 GO:0031090 GO:0031625 GO:0031967 GO:0031969 GO:0031975 GO:0032446 GO:0032787 GO:0033554 GO:0033559 GO:0034976 GO:0036211 GO:0036503 GO:0042170 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044283 GO:0044389 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046471 GO:0046486 GO:0050896 GO:0051603 GO:0051716 GO:0052637 GO:0055114 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0072330 GO:0080167 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901698

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TMEM189_B_dmain

Protein Analysis

306

Amino Acids

33.94

Weight (kDa)

6.78

Isoelectric Point (pI)

46.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipid_desat	PF10520	117 - 285	2.1e-70	Lipid desaturase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011204)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27030
fragaria_vesca	FvH4_3g22400
malus_domestica	MD03G1205300.v1.1 MD11G1221200.v1.1
prunus_persica	Prupe.4G210500_v2.0.a1 Prupe.4G210600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0039071
rosa_laevigata	RLG00000033888 RLG00000033890 RLG00000033891
rosa_multiflora	Rmu_co8409277.1_g000001 Rmu_sc0000533.1_g000033 Rmu_sc0007753.1_g000004
rosa_roxburghii	Rroxscaffold_1G00042040
rosa_rugosa	Rorug05G0176300
rosa_samantha	Rh5AG262700 Rh5BG267500 Rh5CG300200 Rh5DG273900
rosa_wichuraiana	Rw5G024560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	407
AccBSI	CCGCTC	1 cut(s)	252
AccI	GTMKAC	1 cut(s)	214
AccIII	TCCGGA	1 cut(s)	371
AciI	CCGC	6 cut(s)	79, 149, 161, 252, 335, 359
AclWI	GGATC	2 cut(s)	369, 557
AcoI	YGGCCR	2 cut(s)	191, 464
AdeI	CACNNNGTG	1 cut(s)	236
AfiI	CCNNNNNNNGG	2 cut(s)	241, 425
AgsI	TTSAA	3 cut(s)	449, 803, 881
AjnI	CCWGG	2 cut(s)	152, 510
AjuI	GAANNNNNNNTTGG	2 cut(s)	764, 796
AleI	CACNNNNGTG	1 cut(s)	263
AluBI	AGCT	6 cut(s)	25, 136, 166, 281, 806, 867
AluI	AGCT	6 cut(s)	25, 136, 166, 281, 806, 867
Alw21I	GWGCWC	3 cut(s)	260, 270, 433
Alw26I	GTCTC	1 cut(s)	884
Alw44I	GTGCAC	2 cut(s)	256, 429
AlwI	GGATC	2 cut(s)	369, 557
Ama87I	CYCGRG	2 cut(s)	208, 365
Aor13HI	TCCGGA	1 cut(s)	371
AoxI	GGCC	4 cut(s)	191, 318, 451, 464
ApaLI	GTGCAC	2 cut(s)	256, 429
ApeKI	GCWGC	3 cut(s)	157, 163, 299
ArsI	GACNNNNNNTTYG	4 cut(s)	123, 155, 539, 571
Asp700I	GAANNNNTTC	1 cut(s)	444
AspA2I	CCTAGG	1 cut(s)	845
AspLEI	GCGC	2 cut(s)	486, 721
AspS9I	GGNCC	2 cut(s)	318, 369
AsuC2I	CCSGG	1 cut(s)	190
AsuHPI	GGTGA	1 cut(s)	168
AvaI	CYCGRG	2 cut(s)	208, 365
AvaII	GGWCC	1 cut(s)	369
AvrII	CCTAGG	1 cut(s)	845
BaeGI	GKGCMC	2 cut(s)	260, 433
BalI	TGGCCA	1 cut(s)	466
BanI	GGYRCC	1 cut(s)	407
BanII	GRGCYC	1 cut(s)	637
BauI	CACGAG	1 cut(s)	516
Bbv12I	GWGCWC	3 cut(s)	260, 270, 433
BbvI	GCAGC	3 cut(s)	144, 175, 311
BceAI	ACGGC	1 cut(s)	655
BciT130I	CCWGG	2 cut(s)	154, 512
BcnI	CCSGG	1 cut(s)	190
BcoDI	GTCTC	1 cut(s)	884
BfaI	CTAG	3 cut(s)	278, 846, 864
BfoI	RGCGCY	1 cut(s)	487
BisI	GCNGC	4 cut(s)	158, 161, 164, 300
BlnI	CCTAGG	1 cut(s)	845
BlsI	GCNGC	4 cut(s)	159, 162, 165, 301
Bme1390I	CCNGG	3 cut(s)	154, 190, 512
Bme18I	GGWCC	1 cut(s)	369
BmeT110I	CYCGRG	2 cut(s)	208, 365
BmgT120I	GGNCC	2 cut(s)	318, 369
BmiI	GGNNCC	3 cut(s)	171, 370, 409
BmrFI	CCNGG	3 cut(s)	154, 190, 512
BmsI	GCATC	1 cut(s)	186
BoxI	GACNNNNGTC	2 cut(s)	210, 847
BpmI	CTGGAG	1 cut(s)	54
BpuMI	CCSGG	1 cut(s)	190
BsaJI	CCNNGG	4 cut(s)	152, 467, 510, 845
BsaWI	WCCGGW	1 cut(s)	371
Bsc4I	CCNNNNNNNGG	2 cut(s)	241, 425
Bse118I	RCCGGY	2 cut(s)	651, 726
Bse1I	ACTGG	3 cut(s)	71, 122, 487
Bse3DI	GCAATG	2 cut(s)	91, 674
BseAI	TCCGGA	1 cut(s)	371
BseBI	CCWGG	2 cut(s)	154, 512
BseDI	CCNNGG	4 cut(s)	152, 467, 510, 845
BseLI	CCNNNNNNNGG	2 cut(s)	241, 425
BseMI	GCAATG	2 cut(s)	91, 674
BseMII	CTCAG	2 cut(s)	861, 879
BseNI	ACTGG	3 cut(s)	71, 122, 487
BseRI	GAGGAG	1 cut(s)	17
BseSI	GKGCMC	2 cut(s)	260, 433
BseX3I	CGGCCG	1 cut(s)	191
BseXI	GCAGC	3 cut(s)	144, 175, 311
BsgI	GTGCAG	1 cut(s)	318
Bsh1285I	CGRYCG	2 cut(s)	194, 707
BshFI	GGCC	4 cut(s)	193, 320, 453, 466
BshNI	GGYRCC	1 cut(s)	407
BsiEI	CGRYCG	2 cut(s)	194, 707
BsiHKAI	GWGCWC	3 cut(s)	260, 270, 433
BsiHKCI	CYCGRG	2 cut(s)	208, 365
BsiSI	CCGG	4 cut(s)	190, 372, 652, 727
BslFI	GGGAC	1 cut(s)	110
BslI	CCNNNNNNNGG	2 cut(s)	241, 425
BsmAI	GTCTC	1 cut(s)	884
BsmBI	CGTCTC	1 cut(s)	884
BsmFI	GGGAC	1 cut(s)	110
BsnI	GGCC	4 cut(s)	193, 320, 453, 466
BsoBI	CYCGRG	2 cut(s)	208, 365
Bsp1286I	GDGCHC	4 cut(s)	260, 270, 433, 637
Bsp13I	TCCGGA	1 cut(s)	371
Bsp143I	GATC	4 cut(s)	361, 562, 704, 816
Bsp19I	CCATGG	1 cut(s)	467
BspACI	CCGC	6 cut(s)	79, 149, 161, 252, 335, 359
BspANI	GGCC	4 cut(s)	193, 320, 453, 466
BspCNI	CTCAG	2 cut(s)	862, 880
BspEI	TCCGGA	1 cut(s)	371
BspLI	GGNNCC	3 cut(s)	171, 370, 409
BspPI	GGATC	2 cut(s)	369, 557
BspT107I	GGYRCC	1 cut(s)	407
BsrBI	CCGCTC	1 cut(s)	252
BsrDI	GCAATG	2 cut(s)	91, 674
BsrFI	RCCGGY	2 cut(s)	651, 726
BsrI	ACTGG	3 cut(s)	71, 122, 487
BssAI	RCCGGY	2 cut(s)	651, 726
BssECI	CCNNGG	4 cut(s)	152, 467, 510, 845
BssMI	GATC	4 cut(s)	361, 562, 704, 816
BssSI	CACGAG	1 cut(s)	516
BssT1I	CCWWGG	2 cut(s)	467, 845
Bst2BI	CACGAG	1 cut(s)	516
Bst2UI	CCWGG	2 cut(s)	154, 512
Bst4CI	ACNGT	4 cut(s)	88, 236, 265, 535
Bst6I	CTCTTC	1 cut(s)	876
BstC8I	GCNNGC	3 cut(s)	250, 337, 653
BstDEI	CTNAG	4 cut(s)	480, 544, 870, 888
BstDSI	CCRYGG	1 cut(s)	467
BstH2I	RGCGCY	1 cut(s)	487
BstHHI	GCGC	2 cut(s)	486, 721
BstKTI	GATC	4 cut(s)	364, 565, 707, 819
BstMAI	GTCTC	1 cut(s)	884
BstMBI	GATC	4 cut(s)	361, 562, 704, 816
BstMCI	CGRYCG	2 cut(s)	194, 707
BstMWI	GCNNNNNNNGC	6 cut(s)	157, 163, 245, 600, 648, 727
BstNI	CCWGG	2 cut(s)	154, 512
BstNSI	RCATGY	1 cut(s)	509
BstPAI	GACNNNNGTC	2 cut(s)	210, 847
BstSCI	CCNGG	3 cut(s)	152, 188, 510
BstSLI	GKGCMC	2 cut(s)	260, 433
BstV1I	GCAGC	3 cut(s)	144, 175, 311
BstX2I	RGATCY	1 cut(s)	361
BstYI	RGATCY	1 cut(s)	361
BstZI	CGGCCG	1 cut(s)	191
BsuRI	GGCC	4 cut(s)	193, 320, 453, 466
BtgI	CCRYGG	1 cut(s)	467
BtgZI	GCGATG	1 cut(s)	169
BtsIMutI	CAGTG	2 cut(s)	261, 540
Cac8I	GCNNGC	3 cut(s)	250, 337, 653
CfoI	GCGC	2 cut(s)	486, 721
Cfr10I	RCCGGY	2 cut(s)	651, 726
Cfr13I	GGNCC	2 cut(s)	318, 369
CspCI	CAANNNNNGTGG	2 cut(s)	385, 420
CviAII	CATG	7 cut(s)	47, 225, 240, 468, 506, 575, 626
DdeI	CTNAG	4 cut(s)	480, 544, 870, 888
DpnI	GATC	4 cut(s)	363, 564, 706, 818
DpnII	GATC	4 cut(s)	361, 562, 704, 816
DraIII	CACNNNGTG	1 cut(s)	236
EaeI	YGGCCR	2 cut(s)	191, 464
EagI	CGGCCG	1 cut(s)	191
Eam1104I	CTCTTC	1 cut(s)	876
EarI	CTCTTC	1 cut(s)	876
EciI	GGCGGA	1 cut(s)	374
EclXI	CGGCCG	1 cut(s)	191
Eco130I	CCWWGG	2 cut(s)	467, 845
Eco24I	GRGCYC	1 cut(s)	637
Eco47I	GGWCC	1 cut(s)	369
Eco52I	CGGCCG	1 cut(s)	191
Eco88I	CYCGRG	2 cut(s)	208, 365
EcoRII	CCWGG	2 cut(s)	152, 510
EcoT14I	CCWWGG	2 cut(s)	467, 845
EcoT38I	GRGCYC	1 cut(s)	637
ErhI	CCWWGG	2 cut(s)	467, 845
Esp3I	CGTCTC	1 cut(s)	884
FaeI	CATG	7 cut(s)	50, 228, 243, 471, 509, 578, 629
FaqI	GGGAC	1 cut(s)	110
FatI	CATG	7 cut(s)	46, 224, 239, 467, 505, 574, 625
FauI	CCCGC	2 cut(s)	245, 328
FauNDI	CATATG	1 cut(s)	314
FblI	GTMKAC	1 cut(s)	214
Fnu4HI	GCNGC	4 cut(s)	158, 161, 164, 300
FriOI	GRGCYC	1 cut(s)	637
Fsp4HI	GCNGC	4 cut(s)	158, 161, 164, 300
FspBI	CTAG	3 cut(s)	278, 846, 864
GlaI	GCGC	2 cut(s)	485, 720
GluI	GCNGC	4 cut(s)	158, 161, 164, 300
GsuI	CTGGAG	1 cut(s)	54
HaeII	RGCGCY	1 cut(s)	487
HaeIII	GGCC	4 cut(s)	193, 320, 453, 466
HapII	CCGG	4 cut(s)	190, 372, 652, 727
HhaI	GCGC	2 cut(s)	486, 721
Hin1II	CATG	7 cut(s)	50, 228, 243, 471, 509, 578, 629
Hin6I	GCGC	2 cut(s)	484, 719
HinP1I	GCGC	2 cut(s)	484, 719
HincII	GTYRAC	1 cut(s)	617
HindII	GTYRAC	1 cut(s)	617
HindIII	AAGCTT	1 cut(s)	804
HinfI	GANTC	2 cut(s)	211, 683
HpaII	CCGG	4 cut(s)	190, 372, 652, 727
HphI	GGTGA	1 cut(s)	168
Hpy166II	GTNNAC	7 cut(s)	52, 215, 258, 379, 431, 617, 860
Hpy188I	TCNGA	2 cut(s)	871, 901
Hpy188III	TCNNGA	2 cut(s)	372, 702
Hpy8I	GTNNAC	7 cut(s)	52, 215, 258, 379, 431, 617, 860
HpyAV	CCTTC	1 cut(s)	787
HpyCH4III	ACNGT	4 cut(s)	88, 236, 265, 535
HpyCH4V	TGCA	8 cut(s)	239, 258, 299, 431, 603, 679, 753, 908
HpyF10VI	GCNNNNNNNGC	6 cut(s)	157, 163, 245, 600, 648, 727
HpyF3I	CTNAG	4 cut(s)	480, 544, 870, 888
Hsp92II	CATG	7 cut(s)	50, 228, 243, 471, 509, 578, 629
HspAI	GCGC	2 cut(s)	484, 719
Kpn2I	TCCGGA	1 cut(s)	371
KroI	GCCGGC	1 cut(s)	651
KroNI	GCCGGC	1 cut(s)	653
Kzo9I	GATC	4 cut(s)	361, 562, 704, 816
LmnI	GCTCC	2 cut(s)	30, 101
Lsp1109I	GCAGC	3 cut(s)	144, 175, 311
LweI	GCATC	1 cut(s)	186
MaeI	CTAG	3 cut(s)	278, 846, 864
MaeIII	GTNAC	1 cut(s)	228
MalI	GATC	4 cut(s)	363, 564, 706, 818
MbiI	CCGCTC	1 cut(s)	252
MboI	GATC	4 cut(s)	361, 562, 704, 816
MboII	GAAGA	2 cut(s)	563, 893
MfeI	CAATTG	1 cut(s)	913
MflI	RGATCY	1 cut(s)	361
MhlI	GDGCHC	4 cut(s)	260, 270, 433, 637
MlsI	TGGCCA	1 cut(s)	466
MluCI	AATT	5 cut(s)	304, 390, 420, 620, 913
MluNI	TGGCCA	1 cut(s)	466
MlyI	GAGTC	1 cut(s)	220
MnlI	CCTC	6 cut(s)	38, 78, 86, 184, 421, 877
Mox20I	TGGCCA	1 cut(s)	466
MroI	TCCGGA	1 cut(s)	371
MroNI	GCCGGC	1 cut(s)	651
MroXI	GAANNNNTTC	1 cut(s)	444
MscI	TGGCCA	1 cut(s)	466
MseI	TTAA	2 cut(s)	828, 839
MslI	CAYNNNNRTG	2 cut(s)	263, 313
Msp20I	TGGCCA	1 cut(s)	466
MspA1I	CMGCKG	1 cut(s)	166
MspI	CCGG	4 cut(s)	190, 372, 652, 727
MspR9I	CCNGG	3 cut(s)	154, 190, 512
MunI	CAATTG	1 cut(s)	913
MvaI	CCWGG	2 cut(s)	154, 512
MwoI	GCNNNNNNNGC	6 cut(s)	157, 163, 245, 600, 648, 727
NaeI	GCCGGC	1 cut(s)	653
NciI	CCSGG	1 cut(s)	190
NcoI	CCATGG	1 cut(s)	467
NdeI	CATATG	1 cut(s)	314
NdeII	GATC	4 cut(s)	361, 562, 704, 816
NgoMIV	GCCGGC	1 cut(s)	651
NlaIII	CATG	7 cut(s)	50, 228, 243, 471, 509, 578, 629
NlaIV	GGNNCC	3 cut(s)	171, 370, 409
NmuCI	GTSAC	1 cut(s)	228
NspI	RCATGY	1 cut(s)	509
OliI	CACNNNNGTG	1 cut(s)	263
PdiI	GCCGGC	1 cut(s)	653
PdmI	GAANNNNTTC	1 cut(s)	444
PfeI	GAWTC	1 cut(s)	683
PkrI	GCNGC	4 cut(s)	159, 162, 165, 301
Ple19I	CGATCG	1 cut(s)	707
PleI	GAGTC	1 cut(s)	219
PpsI	GAGTC	1 cut(s)	219
PshAI	GACNNNNGTC	2 cut(s)	210, 847
Psp6I	CCWGG	2 cut(s)	152, 510
PspGI	CCWGG	2 cut(s)	152, 510
PspN4I	GGNNCC	3 cut(s)	171, 370, 409
PspPI	GGNCC	2 cut(s)	318, 369
PsuI	RGATCY	1 cut(s)	361
PvuI	CGATCG	1 cut(s)	707
PvuII	CAGCTG	1 cut(s)	166
RseI	CAYNNNNRTG	2 cut(s)	263, 313
SaqAI	TTAA	2 cut(s)	828, 839
SatI	GCNGC	4 cut(s)	158, 161, 164, 300
Sau3AI	GATC	4 cut(s)	361, 562, 704, 816
Sau96I	GGNCC	2 cut(s)	318, 369
SchI	GAGTC	1 cut(s)	220
ScrFI	CCNGG	3 cut(s)	154, 190, 512
SduI	GDGCHC	4 cut(s)	260, 270, 433, 637
SfaNI	GCATC	1 cut(s)	186
SinI	GGWCC	1 cut(s)	369
SmiMI	CAYNNNNRTG	2 cut(s)	263, 313
Sse9I	AATT	5 cut(s)	304, 390, 420, 620, 913
SsiI	CCGC	6 cut(s)	79, 149, 161, 252, 335, 359
SspMI	CTAG	3 cut(s)	278, 846, 864
StyD4I	CCNGG	3 cut(s)	152, 188, 510
StyI	CCWWGG	2 cut(s)	467, 845
TaaI	ACNGT	4 cut(s)	88, 236, 265, 535
TaqI	TCGA	2 cut(s)	144, 703
TasI	AATT	5 cut(s)	304, 390, 420, 620, 913
TauI	GCSGC	1 cut(s)	163
TfiI	GAWTC	1 cut(s)	683
Tru1I	TTAA	2 cut(s)	828, 839
Tru9I	TTAA	2 cut(s)	828, 839
TscAI	CASTG	2 cut(s)	268, 540
TseFI	GTSAC	1 cut(s)	228
TseI	GCWGC	3 cut(s)	157, 163, 299
Tsp45I	GTSAC	1 cut(s)	228
TspDTI	ATGAA	2 cut(s)	563, 614
TspRI	CASTG	2 cut(s)	268, 540
VneI	GTGCAC	2 cut(s)	256, 429
VpaK11BI	GGWCC	1 cut(s)	369
XceI	RCATGY	1 cut(s)	509
XmaJI	CCTAGG	1 cut(s)	845
XmiI	GTMKAC	1 cut(s)	214
XmnI	GAANNNNTTC	1 cut(s)	444
XspI	CTAG	3 cut(s)	278, 846, 864

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.