Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0011204

Physical Location & Seq

Genomic Coordinates Forward (+)

60046676 .. 60047518

Gene Structure 843 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00042040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 843 bp

ATGTCTATCTTCACTCAAACCAAGCTCCTCCCAAGCCCCAAACACCATGTTCACACAATATGCCACTCTCCAATCCTCCGAACAACTGTACATTGCTCTGTCTCCACCACCGCAAAGCCGAAGCCAGTCCCCGAAAAGCTCATCATCGAACCTCCCCGGCTGCAGCAGCTGGAACCGTCACCGGACAACATCCGGCCGATGCTCAATGACCCGAGTCTACTATCAACGTGGTCTCACCGTGCGTGGGTAGCGAGCGGGTGCACTACTGTGCTCATTTCTCTAGCTAAATCCGTAGCGGGTGCAGCCCATTCACACATATGGGCCGAGCCCATTTTAGCAGGCTTGGTCGGGTACGTGTTGGCGGATCTCGGATCCGGAGTTTACCATTGGGGCATTGACAACTATGGTGGTGCCTCAACTCCAATTTTTGGTGCCCAAATAGAGGCATTCCAAGGTCACCATAAGTGGCCCTGGACAATCACTAAGCGCCAATTTGCTAACAATTTACATGCCTTGGCTCGTGTCATAACTTTCACAGTGCTTCCCTTAGACCTTGGCTTTGATGATCCTATTTTTCATGGCTTTGTTGCTGTTTGCTCTGGTTGCATTATGTTTAGCCAGCAATTTCATGCTTGGGCTCATGGCACAAAGAGCCGGCTACCTCCATTGGTGCTGGCATTGCAAGATTCCGGCTTACTGATATCTCGGTCTCAACATTCGGAGCACCACCGGCAACCTTATAACAACAACTACTGCATAGTAAGTGGAGTTTGGAACGAGTTTTTGGATAAGCAGAAGGTATTTGAAGCGTTGGAGATGATCTTGTTTTTAAGCAAGGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009941 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016053 GO:0016491 GO:0016567 GO:0016705 GO:0016740 GO:0019538 GO:0019637 GO:0019752 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030433 GO:0031090 GO:0031625 GO:0031967 GO:0031969 GO:0031975 GO:0032446 GO:0032787 GO:0033554 GO:0033559 GO:0034976 GO:0036211 GO:0036503 GO:0042170 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044283 GO:0044389 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046471 GO:0046486 GO:0050896 GO:0051603 GO:0051716 GO:0052637 GO:0055114 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0072330 GO:0080167 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901698

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TMEM189_B_dmain

Protein Analysis

280

Amino Acids

31.05

Weight (kDa)

8.53

Isoelectric Point (pI)

43.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipid_desat	PF10520	118 - 279	1.9e-66	Lipid desaturase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011204)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27030
fragaria_vesca	FvH4_3g22400
malus_domestica	MD03G1205300.v1.1 MD11G1221200.v1.1
prunus_persica	Prupe.4G210500_v2.0.a1 Prupe.4G210600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0039071
rosa_laevigata	RLG00000033888 RLG00000033890 RLG00000033891
rosa_multiflora	Rmu_co8409277.1_g000001 Rmu_sc0000533.1_g000033 Rmu_sc0007753.1_g000004
rosa_roxburghii	Rroxscaffold_1G00042040
rosa_rugosa	Rorug05G0176300
rosa_samantha	Rh5AG262700 Rh5BG267500 Rh5CG300200 Rh5DG273900
rosa_wichuraiana	Rw5G024560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	741
AccB1I	GGYRCC	2 cut(s)	410, 431
AccB7I	CCANNNNNTGG	1 cut(s)	428
AccBSI	CCGCTC	1 cut(s)	255
AccI	GTMKAC	1 cut(s)	217
AccIII	TCCGGA	1 cut(s)	374
AciI	CCGC	4 cut(s)	111, 255, 296, 362
AclWI	GGATC	4 cut(s)	366, 372, 379, 560
AcoI	YGGCCR	1 cut(s)	194
AfaI	GTAC	2 cut(s)	90, 353
AfiI	CCNNNNNNNGG	3 cut(s)	244, 428, 442
AflIII	ACRYGT	1 cut(s)	354
AgsI	TTSAA	1 cut(s)	806
AjnI	CCWGG	1 cut(s)	470
AjuI	GAANNNNNNNTTGG	2 cut(s)	767, 799
AleI	CACNNNNGTG	1 cut(s)	266
AluBI	AGCT	4 cut(s)	25, 139, 169, 284
AluI	AGCT	4 cut(s)	25, 139, 169, 284
Alw21I	GWGCWC	3 cut(s)	263, 273, 726
Alw26I	GTCTC	3 cut(s)	106, 237, 714
Alw44I	GTGCAC	1 cut(s)	259
AlwI	GGATC	4 cut(s)	366, 372, 379, 560
AlwNI	CAGNNNCTG	1 cut(s)	169
Ama87I	CYCGRG	1 cut(s)	211
Aor13HI	TCCGGA	1 cut(s)	374
AoxI	GGCC	3 cut(s)	194, 321, 467
ApaLI	GTGCAC	1 cut(s)	259
ApeKI	GCWGC	4 cut(s)	160, 163, 166, 302
ArsI	GACNNNNNNTTYG	2 cut(s)	542, 574
AspLEI	GCGC	1 cut(s)	489
AspS9I	GGNCC	2 cut(s)	321, 468
AsuC2I	CCSGG	1 cut(s)	157
AsuHPI	GGTGA	3 cut(s)	171, 227, 449
AvaI	CYCGRG	1 cut(s)	211
BaeGI	GKGCMC	2 cut(s)	263, 436
BamHI	GGATCC	1 cut(s)	371
BanI	GGYRCC	2 cut(s)	410, 431
BanII	GRGCYC	2 cut(s)	330, 640
BauI	CACGAG	1 cut(s)	519
Bbv12I	GWGCWC	3 cut(s)	263, 273, 726
BbvI	GCAGC	4 cut(s)	147, 175, 178, 314
BciT130I	CCWGG	1 cut(s)	472
BcnI	CCSGG	1 cut(s)	157
BcoDI	GTCTC	3 cut(s)	106, 237, 714
BfaI	CTAG	1 cut(s)	281
BfmI	CTRYAG	1 cut(s)	161
BfoI	RGCGCY	1 cut(s)	490
BisI	GCNGC	4 cut(s)	161, 164, 167, 303
BlsI	GCNGC	4 cut(s)	162, 165, 168, 304
Bme1390I	CCNGG	2 cut(s)	157, 472
BmeT110I	CYCGRG	1 cut(s)	211
BmgT120I	GGNCC	2 cut(s)	321, 468
BmiI	GGNNCC	4 cut(s)	174, 373, 412, 433
BmrFI	CCNGG	2 cut(s)	157, 472
BmsI	GCATC	1 cut(s)	189
BoxI	GACNNNNGTC	1 cut(s)	213
BpuMI	CCSGG	1 cut(s)	157
BsaAI	YACGTR	1 cut(s)	355
BsaI	GGTCTC	2 cut(s)	237, 714
BsaJI	CCNNGG	5 cut(s)	155, 451, 470, 513, 553
BsaWI	WCCGGW	2 cut(s)	181, 374
Bsc4I	CCNNNNNNNGG	3 cut(s)	244, 428, 442
Bse118I	RCCGGY	2 cut(s)	654, 729
Bse1I	ACTGG	1 cut(s)	125
Bse3DI	GCAATG	2 cut(s)	91, 677
BseAI	TCCGGA	1 cut(s)	374
BseBI	CCWGG	1 cut(s)	472
BseDI	CCNNGG	5 cut(s)	155, 451, 470, 513, 553
BseGI	GGATG	1 cut(s)	189
BseLI	CCNNNNNNNGG	3 cut(s)	244, 428, 442
BseMI	GCAATG	2 cut(s)	91, 677
BseNI	ACTGG	1 cut(s)	125
BseRI	GAGGAG	1 cut(s)	17
BseSI	GKGCMC	2 cut(s)	263, 436
BseX3I	CGGCCG	1 cut(s)	194
BseXI	GCAGC	4 cut(s)	147, 175, 178, 314
BsgI	GTGCAG	1 cut(s)	321
Bsh1285I	CGRYCG	1 cut(s)	197
BshFI	GGCC	3 cut(s)	196, 323, 469
BshNI	GGYRCC	2 cut(s)	410, 431
BsiEI	CGRYCG	1 cut(s)	197
BsiHKAI	GWGCWC	3 cut(s)	263, 273, 726
BsiHKCI	CYCGRG	1 cut(s)	211
BsiSI	CCGG	7 cut(s)	157, 182, 193, 375, 655, 690, 730
BslFI	GGGAC	1 cut(s)	113
BslI	CCNNNNNNNGG	3 cut(s)	244, 428, 442
BsmAI	GTCTC	3 cut(s)	106, 237, 714
BsmFI	GGGAC	1 cut(s)	113
BsmI	GAATGC	1 cut(s)	446
BsnI	GGCC	3 cut(s)	196, 323, 469
Bso31I	GGTCTC	2 cut(s)	237, 714
BsoBI	CYCGRG	1 cut(s)	211
Bsp1286I	GDGCHC	6 cut(s)	263, 273, 330, 436, 640, 726
Bsp13I	TCCGGA	1 cut(s)	374
Bsp1407I	TGTACA	1 cut(s)	88
Bsp143I	GATC	4 cut(s)	364, 371, 565, 819
BspACI	CCGC	4 cut(s)	111, 255, 296, 362
BspANI	GGCC	3 cut(s)	196, 323, 469
BspEI	TCCGGA	1 cut(s)	374
BspLI	GGNNCC	4 cut(s)	174, 373, 412, 433
BspMAI	CTGCAG	1 cut(s)	165
BspPI	GGATC	4 cut(s)	366, 372, 379, 560
BspT107I	GGYRCC	2 cut(s)	410, 431
BspTNI	GGTCTC	2 cut(s)	237, 714
BsrBI	CCGCTC	1 cut(s)	255
BsrDI	GCAATG	2 cut(s)	91, 677
BsrFI	RCCGGY	2 cut(s)	654, 729
BsrGI	TGTACA	1 cut(s)	88
BsrI	ACTGG	1 cut(s)	125
BssAI	RCCGGY	2 cut(s)	654, 729
BssECI	CCNNGG	5 cut(s)	155, 451, 470, 513, 553
BssMI	GATC	4 cut(s)	364, 371, 565, 819
BssSI	CACGAG	1 cut(s)	519
BssT1I	CCWWGG	3 cut(s)	451, 513, 553
Bst2BI	CACGAG	1 cut(s)	519
Bst2UI	CCWGG	1 cut(s)	472
Bst4CI	ACNGT	5 cut(s)	88, 177, 239, 268, 538
BstAUI	TGTACA	1 cut(s)	88
BstBAI	YACGTR	1 cut(s)	355
BstC8I	GCNNGC	5 cut(s)	253, 340, 620, 656, 675
BstDEI	CTNAG	2 cut(s)	483, 547
BstEII	GGTNACC	1 cut(s)	455
BstF5I	GGATG	1 cut(s)	189
BstH2I	RGCGCY	1 cut(s)	490
BstHHI	GCGC	1 cut(s)	489
BstKTI	GATC	4 cut(s)	367, 374, 568, 822
BstMAI	GTCTC	3 cut(s)	106, 237, 714
BstMBI	GATC	4 cut(s)	364, 371, 565, 819
BstMCI	CGRYCG	1 cut(s)	197
BstMWI	GCNNNNNNNGC	7 cut(s)	166, 248, 302, 603, 651, 679, 730
BstNI	CCWGG	1 cut(s)	472
BstNSI	RCATGY	1 cut(s)	512
BstPAI	GACNNNNGTC	1 cut(s)	213
BstPI	GGTNACC	1 cut(s)	455
BstSCI	CCNGG	2 cut(s)	155, 470
BstSFI	CTRYAG	1 cut(s)	161
BstSLI	GKGCMC	2 cut(s)	263, 436
BstV1I	GCAGC	4 cut(s)	147, 175, 178, 314
BstX2I	RGATCY	2 cut(s)	364, 371
BstYI	RGATCY	2 cut(s)	364, 371
BstZI	CGGCCG	1 cut(s)	194
BsuRI	GGCC	3 cut(s)	196, 323, 469
BtsCI	GGATG	1 cut(s)	189
BtsIMutI	CAGTG	1 cut(s)	543
Cac8I	GCNNGC	5 cut(s)	253, 340, 620, 656, 675
CaiI	CAGNNNCTG	1 cut(s)	169
CfoI	GCGC	1 cut(s)	489
Cfr10I	RCCGGY	2 cut(s)	654, 729
Cfr13I	GGNCC	2 cut(s)	321, 468
Csp6I	GTAC	2 cut(s)	89, 352
CspCI	CAANNNNNGTGG	2 cut(s)	388, 423
CviAII	CATG	5 cut(s)	47, 509, 578, 629, 641
CviQI	GTAC	2 cut(s)	89, 352
DdeI	CTNAG	2 cut(s)	483, 547
DpnI	GATC	4 cut(s)	366, 373, 567, 821
DpnII	GATC	4 cut(s)	364, 371, 565, 819
EaeI	YGGCCR	1 cut(s)	194
EagI	CGGCCG	1 cut(s)	194
EciI	GGCGGA	1 cut(s)	377
EclXI	CGGCCG	1 cut(s)	194
Eco130I	CCWWGG	3 cut(s)	451, 513, 553
Eco24I	GRGCYC	2 cut(s)	330, 640
Eco31I	GGTCTC	2 cut(s)	237, 714
Eco32I	GATATC	1 cut(s)	702
Eco52I	CGGCCG	1 cut(s)	194
Eco88I	CYCGRG	1 cut(s)	211
Eco91I	GGTNACC	1 cut(s)	455
EcoO65I	GGTNACC	1 cut(s)	455
EcoRII	CCWGG	1 cut(s)	470
EcoRV	GATATC	1 cut(s)	702
EcoT14I	CCWWGG	3 cut(s)	451, 513, 553
EcoT38I	GRGCYC	2 cut(s)	330, 640
ErhI	CCWWGG	3 cut(s)	451, 513, 553
FaeI	CATG	5 cut(s)	50, 512, 581, 632, 644
FaqI	GGGAC	1 cut(s)	113
FatI	CATG	5 cut(s)	46, 508, 577, 628, 640
FauI	CCCGC	2 cut(s)	248, 289
FauNDI	CATATG	1 cut(s)	317
FblI	GTMKAC	1 cut(s)	217
Fnu4HI	GCNGC	4 cut(s)	161, 164, 167, 303
FokI	GGATG	1 cut(s)	176
FriOI	GRGCYC	2 cut(s)	330, 640
Fsp4HI	GCNGC	4 cut(s)	161, 164, 167, 303
FspBI	CTAG	1 cut(s)	281
GlaI	GCGC	1 cut(s)	488
GluI	GCNGC	4 cut(s)	161, 164, 167, 303
HaeII	RGCGCY	1 cut(s)	490
HaeIII	GGCC	3 cut(s)	196, 323, 469
HapII	CCGG	7 cut(s)	157, 182, 193, 375, 655, 690, 730
HhaI	GCGC	1 cut(s)	489
Hin1II	CATG	5 cut(s)	50, 512, 581, 632, 644
Hin6I	GCGC	1 cut(s)	487
HinP1I	GCGC	1 cut(s)	487
HinfI	GANTC	2 cut(s)	214, 686
HpaII	CCGG	7 cut(s)	157, 182, 193, 375, 655, 690, 730
HphI	GGTGA	3 cut(s)	171, 227, 449
Hpy166II	GTNNAC	4 cut(s)	52, 218, 261, 382
Hpy188I	TCNGA	3 cut(s)	80, 371, 721
Hpy188III	TCNNGA	1 cut(s)	375
Hpy8I	GTNNAC	4 cut(s)	52, 218, 261, 382
HpyAV	CCTTC	1 cut(s)	790
HpyCH4III	ACNGT	5 cut(s)	88, 177, 239, 268, 538
HpyCH4IV	ACGT	2 cut(s)	227, 354
HpyCH4V	TGCA	6 cut(s)	163, 261, 302, 606, 682, 756
HpyF10VI	GCNNNNNNNGC	7 cut(s)	166, 248, 302, 603, 651, 679, 730
HpyF3I	CTNAG	2 cut(s)	483, 547
HpySE526I	ACGT	2 cut(s)	227, 354
Hsp92II	CATG	5 cut(s)	50, 512, 581, 632, 644
HspAI	GCGC	1 cut(s)	487
Kpn2I	TCCGGA	1 cut(s)	374
KroI	GCCGGC	1 cut(s)	654
KroNI	GCCGGC	1 cut(s)	656
Kzo9I	GATC	4 cut(s)	364, 371, 565, 819
LmnI	GCTCC	2 cut(s)	30, 721
Lsp1109I	GCAGC	4 cut(s)	147, 175, 178, 314
LweI	GCATC	1 cut(s)	189
MaeI	CTAG	1 cut(s)	281
MaeII	ACGT	2 cut(s)	227, 354
MaeIII	GTNAC	2 cut(s)	177, 455
MalI	GATC	4 cut(s)	366, 373, 567, 821
MbiI	CCGCTC	1 cut(s)	255
MboI	GATC	4 cut(s)	364, 371, 565, 819
MflI	RGATCY	2 cut(s)	364, 371
MhlI	GDGCHC	6 cut(s)	263, 273, 330, 436, 640, 726
MluCI	AATT	4 cut(s)	423, 491, 502, 623
MlyI	GAGTC	1 cut(s)	223
MmeI	TCCRAC	1 cut(s)	792
MnlI	CCTC	6 cut(s)	38, 86, 162, 424, 436, 672
MroI	TCCGGA	1 cut(s)	374
MroNI	GCCGGC	1 cut(s)	654
MseI	TTAA	2 cut(s)	830, 841
MslI	CAYNNNNRTG	2 cut(s)	266, 316
MspA1I	CMGCKG	1 cut(s)	169
MspI	CCGG	7 cut(s)	157, 182, 193, 375, 655, 690, 730
MspR9I	CCNGG	2 cut(s)	157, 472
Mva1269I	GAATGC	1 cut(s)	446
MvaI	CCWGG	1 cut(s)	472
MwoI	GCNNNNNNNGC	7 cut(s)	166, 248, 302, 603, 651, 679, 730
NaeI	GCCGGC	1 cut(s)	656
NciI	CCSGG	1 cut(s)	157
NdeI	CATATG	1 cut(s)	317
NdeII	GATC	4 cut(s)	364, 371, 565, 819
NgoMIV	GCCGGC	1 cut(s)	654
NlaIII	CATG	5 cut(s)	50, 512, 581, 632, 644
NlaIV	GGNNCC	4 cut(s)	174, 373, 412, 433
NmeAIII	GCCGAG	1 cut(s)	349
NmuCI	GTSAC	2 cut(s)	177, 455
NspI	RCATGY	1 cut(s)	512
OliI	CACNNNNGTG	1 cut(s)	266
PctI	GAATGC	1 cut(s)	446
PdiI	GCCGGC	1 cut(s)	656
PfeI	GAWTC	1 cut(s)	686
PflMI	CCANNNNNTGG	1 cut(s)	428
PkrI	GCNGC	4 cut(s)	162, 165, 168, 304
PleI	GAGTC	1 cut(s)	222
PpsI	GAGTC	1 cut(s)	222
Ppu21I	YACGTR	1 cut(s)	355
PshAI	GACNNNNGTC	1 cut(s)	213
PsiI	TTATAA	1 cut(s)	741
Psp6I	CCWGG	1 cut(s)	470
PspEI	GGTNACC	1 cut(s)	455
PspGI	CCWGG	1 cut(s)	470
PspN4I	GGNNCC	4 cut(s)	174, 373, 412, 433
PspPI	GGNCC	2 cut(s)	321, 468
PstI	CTGCAG	1 cut(s)	165
PstNI	CAGNNNCTG	1 cut(s)	169
PsuI	RGATCY	2 cut(s)	364, 371
PvuII	CAGCTG	1 cut(s)	169
RsaI	GTAC	2 cut(s)	90, 353
RsaNI	GTAC	2 cut(s)	89, 352
RseI	CAYNNNNRTG	2 cut(s)	266, 316
SaqAI	TTAA	2 cut(s)	830, 841
SatI	GCNGC	4 cut(s)	161, 164, 167, 303
Sau3AI	GATC	4 cut(s)	364, 371, 565, 819
Sau96I	GGNCC	2 cut(s)	321, 468
SchI	GAGTC	1 cut(s)	223
ScrFI	CCNGG	2 cut(s)	157, 472
SduI	GDGCHC	6 cut(s)	263, 273, 330, 436, 640, 726
SfaNI	GCATC	1 cut(s)	189
SfcI	CTRYAG	1 cut(s)	161
SmiMI	CAYNNNNRTG	2 cut(s)	266, 316
Sse9I	AATT	4 cut(s)	423, 491, 502, 623
SsiI	CCGC	4 cut(s)	111, 255, 296, 362
SspMI	CTAG	1 cut(s)	281
StyD4I	CCNGG	2 cut(s)	155, 470
StyI	CCWWGG	3 cut(s)	451, 513, 553
TaaI	ACNGT	5 cut(s)	88, 177, 239, 268, 538
TaiI	ACGT	2 cut(s)	230, 357
TaqI	TCGA	1 cut(s)	147
TaqII	GACCGA	1 cut(s)	696
TasI	AATT	4 cut(s)	423, 491, 502, 623
TatI	WGTACW	1 cut(s)	88
TfiI	GAWTC	1 cut(s)	686
Tru1I	TTAA	2 cut(s)	830, 841
Tru9I	TTAA	2 cut(s)	830, 841
TscAI	CASTG	1 cut(s)	543
TseFI	GTSAC	2 cut(s)	177, 455
TseI	GCWGC	4 cut(s)	160, 163, 166, 302
Tsp45I	GTSAC	2 cut(s)	177, 455
TspDTI	ATGAA	2 cut(s)	566, 617
TspGWI	ACGGA	1 cut(s)	280
TspRI	CASTG	1 cut(s)	543
Van91I	CCANNNNNTGG	1 cut(s)	428
VneI	GTGCAC	1 cut(s)	259
XceI	RCATGY	1 cut(s)	512
XmiI	GTMKAC	1 cut(s)	217
XspI	CTAG	1 cut(s)	281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.