Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0011204

Physical Location & Seq

Genomic Coordinates Forward (+)

28145909 .. 28146874

Gene Structure 966 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1205300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 966 bp

ATGGCTATCTTAACCCAAACCAAGTTCCTCCCAAGTCCCAAAAACCAACTCCACACTTCCCACACTCCAATCCTCCGCACTCGAGTGCATTGCTCCGCCACCAACACCACTACCAAGGCGAACCCCAGCCCGGAAAAGCTAGTCATCAAACCGCCACTACAGTGGCTCGAGCCCTCACCCTCACCCTCACCCTCACCCTCACCCGCCCTACTCAGAGACACTCACACCACCATCCGTCCTCCACGACCCAATGACCCAAGTTTGCTATCCACATGGTCTCACCGTACATGGGTGGCAAGTGGGTGCACTACTGTCCTTGTTTCTCTAGCCAAGTCCATAACTTGTGCAGCTGATTCACACATATGGCTCGAGCCCATTTTGGCAGGCTTGGTTGGGTATGTGCTGGCAGACCTTGGGTCCGGAGTGTACCATTGGGGCATTGACAATTATGGCGATGCCTCGACTCCAATTTTTGGCGTCCAGATCGAAGCGTTCCAAGGCCATCATAAGTGGCCTTGGACAATCACTAAGCGACAATTTGCCAACAATTTACATGCCTTAGCTCGCGTGGTGACTTTCATTGTGCTTCCTATAAACCTTGTGTGTGATGATCCGGTTGTTAATGGGTTTGTTGCAATGTGCTCCGGCTCCATTATGTTTAGCCAACAGTTTCATGCTTGGGCTCACGGCACTAAAAGCCGCCTGCCACCGCTCGTGGTGGCGTTGCAAGATTTAGGAGTCTTGGTATCCCGGTCGCAACATGCAGCACACCATCGGCAGCCTTATAACAACAATTACTGCATTGTAAGTGGGATTTGGAATGAGCTTTTGGATCAGCACAAGGTTTTCGAGGCATTGGAGATGATCTTGTATTTTAACCTAGGGTTAAGACCCAGGTCTTGGAGTGAGCCTACCTCTGAATGGACTGAAGAAATTGAGACTGCTTCACAACTTCCAGCCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009941 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016053 GO:0016491 GO:0016567 GO:0016705 GO:0016740 GO:0019538 GO:0019637 GO:0019752 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030433 GO:0031090 GO:0031625 GO:0031967 GO:0031969 GO:0031975 GO:0032446 GO:0032787 GO:0033554 GO:0033559 GO:0034976 GO:0036211 GO:0036503 GO:0042170 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044283 GO:0044389 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046471 GO:0046486 GO:0050896 GO:0051603 GO:0051716 GO:0052637 GO:0055114 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0072330 GO:0080167 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901698

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TMEM189_B_dmain

Protein Analysis

322

Amino Acids

35.66

Weight (kDa)

7.76

Isoelectric Point (pI)

51.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipid_desat	PF10520	133 - 301	2.5e-70	Lipid desaturase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011204)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27030
fragaria_vesca	FvH4_3g22400
malus_domestica	MD03G1205300.v1.1 MD11G1221200.v1.1
prunus_persica	Prupe.4G210500_v2.0.a1 Prupe.4G210600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0039071
rosa_laevigata	RLG00000033888 RLG00000033890 RLG00000033891
rosa_multiflora	Rmu_co8409277.1_g000001 Rmu_sc0000533.1_g000033 Rmu_sc0007753.1_g000004
rosa_roxburghii	Rroxscaffold_1G00042040
rosa_rugosa	Rorug05G0176300
rosa_samantha	Rh5AG262700 Rh5BG267500 Rh5CG300200 Rh5DG273900
rosa_wichuraiana	Rw5G024560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	786
AccB7I	CCANNNNNTGG	2 cut(s)	473, 900
AccBSI	CCGCTC	1 cut(s)	712
AccII	CGCG	1 cut(s)	567
AccIII	TCCGGA	1 cut(s)	419
AciI	CCGC	6 cut(s)	76, 96, 152, 204, 700, 710
AclWI	GGATC	2 cut(s)	605, 840
AcuI	CTGAAG	1 cut(s)	948
AcyI	GRCGYC	1 cut(s)	477
AfaI	GTAC	2 cut(s)	286, 428
AfiI	CCNNNNNNNGG	5 cut(s)	130, 289, 473, 900, 921
AhdI	GACNNNNNGTC	1 cut(s)	415
AjnI	CCWGG	1 cut(s)	893
AjuI	GAANNNNNNNTTGG	2 cut(s)	812, 844
AleI	CACNNNNGTG	2 cut(s)	83, 160
AluBI	AGCT	4 cut(s)	139, 350, 563, 826
AluI	AGCT	4 cut(s)	139, 350, 563, 826
Alw21I	GWGCWC	2 cut(s)	308, 644
Alw26I	GTCTC	3 cut(s)	210, 282, 932
Alw44I	GTGCAC	1 cut(s)	304
AlwI	GGATC	2 cut(s)	605, 840
Ama87I	CYCGRG	3 cut(s)	81, 167, 368
Aor13HI	TCCGGA	1 cut(s)	419
AoxI	GGCC	2 cut(s)	499, 512
ApaLI	GTGCAC	1 cut(s)	304
ApeKI	GCWGC	3 cut(s)	347, 764, 778
AspA2I	CCTAGG	1 cut(s)	880
AspS9I	GGNCC	1 cut(s)	417
AsuC2I	CCSGG	2 cut(s)	131, 751
AsuHPI	GGTGA	7 cut(s)	168, 174, 180, 186, 192, 272, 583
AvaI	CYCGRG	3 cut(s)	81, 167, 368
AvaII	GGWCC	1 cut(s)	417
AvrII	CCTAGG	1 cut(s)	880
BaeGI	GKGCMC	1 cut(s)	308
BanII	GRGCYC	3 cut(s)	174, 375, 685
BauI	CACGAG	1 cut(s)	713
Bbv12I	GWGCWC	2 cut(s)	308, 644
BbvI	GCAGC	3 cut(s)	359, 776, 790
BccI	CCATC	3 cut(s)	239, 510, 780
BceAI	ACGGC	1 cut(s)	703
BcgI	CGANNNNNNTGC	2 cut(s)	72, 106
BciT130I	CCWGG	1 cut(s)	895
BciVI	GTATCC	1 cut(s)	757
BcnI	CCSGG	2 cut(s)	131, 751
BcoDI	GTCTC	3 cut(s)	210, 282, 932
BfaI	CTAG	3 cut(s)	140, 326, 881
BfmI	CTRYAG	1 cut(s)	158
BfuI	GTATCC	1 cut(s)	757
BisI	GCNGC	4 cut(s)	348, 700, 765, 779
BlnI	CCTAGG	1 cut(s)	880
BlsI	GCNGC	4 cut(s)	349, 701, 766, 780
Bme1390I	CCNGG	3 cut(s)	131, 751, 895
Bme18I	GGWCC	1 cut(s)	417
BmeRI	GACNNNNNGTC	1 cut(s)	415
BmeT110I	CYCGRG	3 cut(s)	81, 167, 368
BmgT120I	GGNCC	1 cut(s)	417
BmiI	GGNNCC	2 cut(s)	418, 649
BmrFI	CCNGG	3 cut(s)	131, 751, 895
BmsI	GCATC	1 cut(s)	445
BoxI	GACNNNNGTC	1 cut(s)	895
BplI	GAGNNNNNCTC	2 cut(s)	899, 931
Bpu10I	CCTNAGC	1 cut(s)	559
BpuMI	CCSGG	2 cut(s)	131, 751
BsaHI	GRCGYC	1 cut(s)	477
BsaI	GGTCTC	1 cut(s)	282
BsaJI	CCNNGG	6 cut(s)	114, 412, 496, 515, 880, 893
BsaWI	WCCGGW	2 cut(s)	419, 613
BsaXI	ACNNNNNCTCC	2 cut(s)	729, 759
Bsc4I	CCNNNNNNNGG	5 cut(s)	130, 289, 473, 900, 921
Bse3DI	GCAATG	2 cut(s)	88, 642
BseAI	TCCGGA	1 cut(s)	419
BseBI	CCWGG	1 cut(s)	895
BseDI	CCNNGG	6 cut(s)	114, 412, 496, 515, 880, 893
BseGI	GGATG	1 cut(s)	231
BseLI	CCNNNNNNNGG	5 cut(s)	130, 289, 473, 900, 921
BseMI	GCAATG	2 cut(s)	88, 642
BseMII	CTCAG	1 cut(s)	226
BseSI	GKGCMC	1 cut(s)	308
BseXI	GCAGC	3 cut(s)	359, 776, 790
BseYI	CCCAGC	1 cut(s)	125
BsgI	GTGCAG	1 cut(s)	366
Bsh1236I	CGCG	1 cut(s)	567
Bsh1285I	CGRYCG	1 cut(s)	755
BshFI	GGCC	2 cut(s)	501, 514
BsiEI	CGRYCG	1 cut(s)	755
BsiHKAI	GWGCWC	2 cut(s)	308, 644
BsiHKCI	CYCGRG	3 cut(s)	81, 167, 368
BsiSI	CCGG	5 cut(s)	131, 420, 614, 645, 751
BslFI	GGGAC	1 cut(s)	21
BslI	CCNNNNNNNGG	5 cut(s)	130, 289, 473, 900, 921
BsmAI	GTCTC	3 cut(s)	210, 282, 932
BsmFI	GGGAC	1 cut(s)	21
BsnI	GGCC	2 cut(s)	501, 514
Bso31I	GGTCTC	1 cut(s)	282
BsoBI	CYCGRG	3 cut(s)	81, 167, 368
Bsp1286I	GDGCHC	5 cut(s)	174, 308, 375, 644, 685
Bsp13I	TCCGGA	1 cut(s)	419
Bsp143I	GATC	4 cut(s)	483, 610, 832, 864
BspACI	CCGC	6 cut(s)	76, 96, 152, 204, 700, 710
BspANI	GGCC	2 cut(s)	501, 514
BspCNI	CTCAG	1 cut(s)	225
BspEI	TCCGGA	1 cut(s)	419
BspFNI	CGCG	1 cut(s)	567
BspLI	GGNNCC	2 cut(s)	418, 649
BspPI	GGATC	2 cut(s)	605, 840
BspTNI	GGTCTC	1 cut(s)	282
BsrBI	CCGCTC	1 cut(s)	712
BsrDI	GCAATG	2 cut(s)	88, 642
BssECI	CCNNGG	6 cut(s)	114, 412, 496, 515, 880, 893
BssMI	GATC	4 cut(s)	483, 610, 832, 864
BssNI	GRCGYC	1 cut(s)	477
BssSI	CACGAG	1 cut(s)	713
BssT1I	CCWWGG	5 cut(s)	114, 412, 496, 515, 880
Bst2BI	CACGAG	1 cut(s)	713
Bst2UI	CCWGG	1 cut(s)	895
Bst4CI	ACNGT	4 cut(s)	162, 284, 313, 669
BstACI	GRCGYC	1 cut(s)	477
BstC8I	GCNNGC	4 cut(s)	385, 405, 565, 704
BstDEI	CTNAG	3 cut(s)	212, 528, 559
BstF5I	GGATG	1 cut(s)	231
BstFNI	CGCG	1 cut(s)	567
BstKTI	GATC	4 cut(s)	486, 613, 835, 867
BstMAI	GTCTC	3 cut(s)	210, 282, 932
BstMBI	GATC	4 cut(s)	483, 610, 832, 864
BstMCI	CGRYCG	1 cut(s)	755
BstMWI	GCNNNNNNNGC	1 cut(s)	696
BstNI	CCWGG	1 cut(s)	895
BstNSI	RCATGY	2 cut(s)	557, 764
BstPAI	GACNNNNGTC	1 cut(s)	895
BstSCI	CCNGG	3 cut(s)	129, 749, 893
BstSFI	CTRYAG	1 cut(s)	158
BstSLI	GKGCMC	1 cut(s)	308
BstUI	CGCG	1 cut(s)	567
BstV1I	GCAGC	3 cut(s)	359, 776, 790
BstXI	CCANNNNNNTGG	1 cut(s)	162
BsuI	GTATCC	1 cut(s)	757
BsuRI	GGCC	2 cut(s)	501, 514
BtgZI	GCGATG	1 cut(s)	468
BtsCI	GGATG	1 cut(s)	231
BtsIMutI	CAGTG	1 cut(s)	167
Cac8I	GCNNGC	4 cut(s)	385, 405, 565, 704
Cfr13I	GGNCC	1 cut(s)	417
CseI	GACGC	1 cut(s)	466
Csp6I	GTAC	2 cut(s)	285, 427
CviAII	CATG	5 cut(s)	273, 288, 554, 674, 761
CviQI	GTAC	2 cut(s)	285, 427
DdeI	CTNAG	3 cut(s)	212, 528, 559
DpnI	GATC	4 cut(s)	485, 612, 834, 866
DpnII	GATC	4 cut(s)	483, 610, 832, 864
DriI	GACNNNNNGTC	1 cut(s)	415
Eam1105I	GACNNNNNGTC	1 cut(s)	415
EciI	GGCGGA	1 cut(s)	85
Eco130I	CCWWGG	5 cut(s)	114, 412, 496, 515, 880
Eco24I	GRGCYC	3 cut(s)	174, 375, 685
Eco31I	GGTCTC	1 cut(s)	282
Eco47I	GGWCC	1 cut(s)	417
Eco57I	CTGAAG	1 cut(s)	948
Eco88I	CYCGRG	3 cut(s)	81, 167, 368
EcoRII	CCWGG	1 cut(s)	893
EcoT14I	CCWWGG	5 cut(s)	114, 412, 496, 515, 880
EcoT38I	GRGCYC	3 cut(s)	174, 375, 685
ErhI	CCWWGG	5 cut(s)	114, 412, 496, 515, 880
FaeI	CATG	5 cut(s)	276, 291, 557, 677, 764
FaqI	GGGAC	1 cut(s)	21
FatI	CATG	5 cut(s)	272, 287, 553, 673, 760
FauI	CCCGC	1 cut(s)	211
FauNDI	CATATG	1 cut(s)	362
Fnu4HI	GCNGC	4 cut(s)	348, 700, 765, 779
FokI	GGATG	1 cut(s)	218
FriOI	GRGCYC	3 cut(s)	174, 375, 685
Fsp4HI	GCNGC	4 cut(s)	348, 700, 765, 779
FspBI	CTAG	3 cut(s)	140, 326, 881
GluI	GCNGC	4 cut(s)	348, 700, 765, 779
GsaI	CCCAGC	1 cut(s)	129
HaeIII	GGCC	2 cut(s)	501, 514
HapII	CCGG	5 cut(s)	131, 420, 614, 645, 751
HgaI	GACGC	1 cut(s)	466
Hin1I	GRCGYC	1 cut(s)	477
Hin1II	CATG	5 cut(s)	276, 291, 557, 677, 764
HinfI	GANTC	3 cut(s)	353, 463, 738
HpaII	CCGG	5 cut(s)	131, 420, 614, 645, 751
HphI	GGTGA	7 cut(s)	168, 174, 180, 186, 192, 272, 583
Hpy166II	GTNNAC	2 cut(s)	306, 427
Hpy188I	TCNGA	2 cut(s)	215, 919
Hpy188III	TCNNGA	2 cut(s)	420, 481
Hpy8I	GTNNAC	2 cut(s)	306, 427
HpyCH4III	ACNGT	4 cut(s)	162, 284, 313, 669
HpyCH4V	TGCA	7 cut(s)	88, 306, 347, 635, 727, 764, 801
HpyF10VI	GCNNNNNNNGC	1 cut(s)	696
HpyF3I	CTNAG	3 cut(s)	212, 528, 559
Hsp92I	GRCGYC	1 cut(s)	477
Hsp92II	CATG	5 cut(s)	276, 291, 557, 677, 764
Kpn2I	TCCGGA	1 cut(s)	419
Kzo9I	GATC	4 cut(s)	483, 610, 832, 864
LmnI	GCTCC	3 cut(s)	98, 647, 653
Lsp1109I	GCAGC	3 cut(s)	359, 776, 790
LweI	GCATC	1 cut(s)	445
MaeI	CTAG	3 cut(s)	140, 326, 881
MaeIII	GTNAC	1 cut(s)	571
MalI	GATC	4 cut(s)	485, 612, 834, 866
MbiI	CCGCTC	1 cut(s)	712
MboI	GATC	4 cut(s)	483, 610, 832, 864
MboII	GAAGA	1 cut(s)	941
MhlI	GDGCHC	5 cut(s)	174, 308, 375, 644, 685
MluCI	AATT	6 cut(s)	445, 468, 536, 547, 793, 933
MlyI	GAGTC	2 cut(s)	457, 747
MroI	TCCGGA	1 cut(s)	419
MseI	TTAA	4 cut(s)	11, 621, 876, 887
MslI	CAYNNNNRTG	3 cut(s)	83, 160, 361
MspA1I	CMGCKG	1 cut(s)	350
MspI	CCGG	5 cut(s)	131, 420, 614, 645, 751
MspR9I	CCNGG	3 cut(s)	131, 751, 895
MvaI	CCWGG	1 cut(s)	895
MvnI	CGCG	1 cut(s)	567
MwoI	GCNNNNNNNGC	1 cut(s)	696
NciI	CCSGG	2 cut(s)	131, 751
NdeI	CATATG	1 cut(s)	362
NdeII	GATC	4 cut(s)	483, 610, 832, 864
NlaIII	CATG	5 cut(s)	276, 291, 557, 677, 764
NlaIV	GGNNCC	2 cut(s)	418, 649
NmuCI	GTSAC	1 cut(s)	571
NspI	RCATGY	2 cut(s)	557, 764
OliI	CACNNNNGTG	2 cut(s)	83, 160
PaeR7I	CTCGAG	3 cut(s)	81, 167, 368
PfeI	GAWTC	1 cut(s)	353
PflMI	CCANNNNNTGG	2 cut(s)	473, 900
PkrI	GCNGC	4 cut(s)	349, 701, 766, 780
PleI	GAGTC	2 cut(s)	457, 746
PpsI	GAGTC	2 cut(s)	457, 746
PshAI	GACNNNNGTC	1 cut(s)	895
PsiI	TTATAA	1 cut(s)	786
Psp6I	CCWGG	1 cut(s)	893
PspFI	CCCAGC	1 cut(s)	125
PspGI	CCWGG	1 cut(s)	893
PspN4I	GGNNCC	2 cut(s)	418, 649
PspPI	GGNCC	1 cut(s)	417
PspXI	VCTCGAGB	3 cut(s)	81, 167, 368
PvuII	CAGCTG	1 cut(s)	350
RsaI	GTAC	2 cut(s)	286, 428
RsaNI	GTAC	2 cut(s)	285, 427
RseI	CAYNNNNRTG	3 cut(s)	83, 160, 361
SaqAI	TTAA	4 cut(s)	11, 621, 876, 887
SatI	GCNGC	4 cut(s)	348, 700, 765, 779
Sau3AI	GATC	4 cut(s)	483, 610, 832, 864
Sau96I	GGNCC	1 cut(s)	417
SchI	GAGTC	2 cut(s)	457, 747
ScrFI	CCNGG	3 cut(s)	131, 751, 895
SduI	GDGCHC	5 cut(s)	174, 308, 375, 644, 685
SfaNI	GCATC	1 cut(s)	445
SfcI	CTRYAG	1 cut(s)	158
Sfr274I	CTCGAG	3 cut(s)	81, 167, 368
SinI	GGWCC	1 cut(s)	417
SlaI	CTCGAG	3 cut(s)	81, 167, 368
SmiMI	CAYNNNNRTG	3 cut(s)	83, 160, 361
SmlI	CTYRAG	3 cut(s)	81, 167, 368
SmoI	CTYRAG	3 cut(s)	81, 167, 368
Sse9I	AATT	6 cut(s)	445, 468, 536, 547, 793, 933
SsiI	CCGC	6 cut(s)	76, 96, 152, 204, 700, 710
SspMI	CTAG	3 cut(s)	140, 326, 881
StyD4I	CCNGG	3 cut(s)	129, 749, 893
StyI	CCWWGG	5 cut(s)	114, 412, 496, 515, 880
TaaI	ACNGT	4 cut(s)	162, 284, 313, 669
TaqI	TCGA	6 cut(s)	82, 168, 369, 461, 486, 849
TasI	AATT	6 cut(s)	445, 468, 536, 547, 793, 933
TauI	GCSGC	1 cut(s)	702
TfiI	GAWTC	1 cut(s)	353
Tru1I	TTAA	4 cut(s)	11, 621, 876, 887
Tru9I	TTAA	4 cut(s)	11, 621, 876, 887
TscAI	CASTG	1 cut(s)	167
TseFI	GTSAC	1 cut(s)	571
TseI	GCWGC	3 cut(s)	347, 764, 778
Tsp45I	GTSAC	1 cut(s)	571
TspDTI	ATGAA	2 cut(s)	568, 662
TspGWI	ACGGA	1 cut(s)	224
TspRI	CASTG	1 cut(s)	167
Van91I	CCANNNNNTGG	2 cut(s)	473, 900
VneI	GTGCAC	1 cut(s)	304
VpaK11BI	GGWCC	1 cut(s)	417
XceI	RCATGY	2 cut(s)	557, 764
XhoI	CTCGAG	3 cut(s)	81, 167, 368
XmaJI	CCTAGG	1 cut(s)	880
XspI	CTAG	3 cut(s)	140, 326, 881

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.