FvH4_3g24840

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0017575
Physical Location & Seq
Forward (+)
17930619 .. 17931048
430 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g24840.t1

Sequence Viewer

Length: 399 bp
ATGGATCGGAGGAGACAAGCGAGTCCAGTGTACACACGGCAGTGGAGCGGAGGCTCCAGCAACCCAGGCTCATCTCCGGCGATGTCGTCGGCGCATCCTCAGTCACGCCTCAATCCTTCCGCCACTGGATTCGATCTCCACCGTCAAGCGCAACTAGAACGTCGCCGCCAAAGCCGCTGCGCAGACGACGATGACGATAATGACGTCAGTTACCGATTCCCTGCTCGGCAACCTCATGCACCGTTTGCTTACTCCAACAATGGCGTCAATAACAGCGGTAGTGGCCTTCCAGCGATTTCGACAACTCGGCCTGCTAGATCTCCATCTCCGGCGGTAATTTCCAAGCCTAATTTAAATTATGATGTTGATTTGAATCATGTGGAAACCAAGTCTGTTTAA

Protein Analysis

133

Amino Acids

14.46

Weight (kDa)

9.73

Isoelectric Point (pI)

85.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017575)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14570 FvH4_3g24840
malus_domestica MD06G1132800.v1.1
rosa_laevigata RLG00000008407 RLG00000016476 RLG00000022590
rosa_multiflora Rmu_sc0003990.1_g000002
rosa_samantha Rh1CG261300 Rh4CG007400 Rh4CG180700 Rh5AG034800 Rh5CG037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 21
AatII GACGTC 1 cut(s) 207
Acc16I TGCGCA 1 cut(s) 181
AccBSI CCGCTC 1 cut(s) 48
AciI CCGC 6 cut(s) 48, 120, 166, 175, 276, 332
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 2 cut(s) 204, 264
AfaI GTAC 1 cut(s) 32
AgsI TTSAA 1 cut(s) 373
AjnI CCWGG 1 cut(s) 64
AleI CACNNNNGTG 1 cut(s) 40
Alw26I GTCTC 1 cut(s) 7
AlwI GGATC 1 cut(s) 12
AoxI GGCC 2 cut(s) 283, 308
ApeKI GCWGC 1 cut(s) 177
AspLEI GCGC 3 cut(s) 94, 151, 182
BbvI GCAGC 1 cut(s) 164
BccI CCATC 1 cut(s) 331
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 7
BfaI CTAG 2 cut(s) 155, 315
BglII AGATCT 1 cut(s) 317
BisI GCNGC 3 cut(s) 166, 175, 178
BlsI GCNGC 3 cut(s) 167, 176, 179
Bme1390I CCNGG 1 cut(s) 66
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 1 cut(s) 103
BpmI CTGGAG 1 cut(s) 40
BsaBI GATNNNNATC 2 cut(s) 322, 372
BsaHI GRCGYC 2 cut(s) 204, 264
BsaJI CCNNGG 1 cut(s) 64
Bse1I ACTGG 2 cut(s) 26, 130
Bse8I GATNNNNATC 2 cut(s) 322, 372
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 1 cut(s) 94
BseJI GATNNNNATC 2 cut(s) 322, 372
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 2 cut(s) 26, 130
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 164
BshFI GGCC 2 cut(s) 285, 310
BsiSI CCGG 2 cut(s) 77, 329
BsmAI GTCTC 1 cut(s) 7
BsnI GGCC 2 cut(s) 285, 310
Bsp1407I TGTACA 1 cut(s) 30
Bsp143I GATC 3 cut(s) 4, 133, 317
BspACI CCGC 6 cut(s) 48, 120, 166, 175, 276, 332
BspANI GGCC 2 cut(s) 285, 310
BspCNI CTCAG 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 1 cut(s) 12
BsrBI CCGCTC 1 cut(s) 48
BsrGI TGTACA 1 cut(s) 30
BsrI ACTGG 2 cut(s) 26, 130
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 3 cut(s) 4, 133, 317
BssNI GRCGYC 2 cut(s) 204, 264
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 2 cut(s) 143, 243
BstACI GRCGYC 2 cut(s) 204, 264
BstAPI GCANNNNNTGC 1 cut(s) 245
BstAUI TGTACA 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 312
BstDEI CTNAG 1 cut(s) 99
BstF5I GGATG 1 cut(s) 94
BstHHI GCGC 3 cut(s) 94, 151, 182
BstKTI GATC 3 cut(s) 7, 136, 320
BstMAI GTCTC 1 cut(s) 7
BstMBI GATC 3 cut(s) 4, 133, 317
BstMWI GCNNNNNNNGC 5 cut(s) 66, 171, 174, 245, 282
BstNI CCWGG 1 cut(s) 66
BstSCI CCNGG 1 cut(s) 64
BstV1I GCAGC 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BsuRI GGCC 2 cut(s) 285, 310
BtgZI GCGATG 1 cut(s) 95
BtsCI GGATG 1 cut(s) 94
BtsI GCAGTG 1 cut(s) 47
BtsIMutI CAGTG 3 cut(s) 33, 47, 123
Cac8I GCNNGC 1 cut(s) 312
CfoI GCGC 3 cut(s) 94, 151, 182
CseI GACGC 1 cut(s) 253
Csp6I GTAC 1 cut(s) 31
CviAII CATG 2 cut(s) 236, 377
CviJI RGCY 6 cut(s) 54, 69, 174, 285, 310, 346
CviKI_1 RGCY 6 cut(s) 54, 69, 174, 285, 310, 346
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 1 cut(s) 99
DpnI GATC 3 cut(s) 6, 135, 319
DpnII GATC 3 cut(s) 4, 133, 317
DraI TTTAAA 1 cut(s) 354
DrdI GACNNNNNNGTC 1 cut(s) 21
DseDI GACNNNNNNGTC 1 cut(s) 21
EciI GGCGGA 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 64
FaeI CATG 2 cut(s) 239, 380
FaiI YATR 3 cut(s) 237, 360, 378
FatI CATG 2 cut(s) 235, 376
Fnu4HI GCNGC 3 cut(s) 166, 175, 178
FokI GGATG 1 cut(s) 81
Fsp4HI GCNGC 3 cut(s) 166, 175, 178
FspBI CTAG 2 cut(s) 155, 315
FspI TGCGCA 1 cut(s) 181
GlaI GCGC 3 cut(s) 93, 150, 181
GluI GCNGC 3 cut(s) 166, 175, 178
GsuI CTGGAG 1 cut(s) 40
HaeIII GGCC 2 cut(s) 285, 310
HapII CCGG 2 cut(s) 77, 329
HgaI GACGC 1 cut(s) 253
HhaI GCGC 3 cut(s) 94, 151, 182
Hin1I GRCGYC 2 cut(s) 204, 264
Hin1II CATG 2 cut(s) 239, 380
Hin6I GCGC 3 cut(s) 92, 149, 180
HinP1I GCGC 3 cut(s) 92, 149, 180
HinfI GANTC 4 cut(s) 22, 129, 216, 373
HpaII CCGG 2 cut(s) 77, 329
Hpy166II GTNNAC 2 cut(s) 31, 33
Hpy188I TCNGA 1 cut(s) 9
Hpy8I GTNNAC 2 cut(s) 31, 33
Hpy99I CGWCG 3 cut(s) 91, 165, 191
HpyAV CCTTC 2 cut(s) 126, 296
HpyCH4III ACNGT 2 cut(s) 143, 243
HpyCH4IV ACGT 2 cut(s) 160, 204
HpyCH4V TGCA 1 cut(s) 239
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 171, 174, 245, 282
HpyF3I CTNAG 1 cut(s) 99
HpySE526I ACGT 2 cut(s) 160, 204
Hsp92I GRCGYC 2 cut(s) 204, 264
Hsp92II CATG 2 cut(s) 239, 380
HspAI GCGC 3 cut(s) 92, 149, 180
Kzo9I GATC 3 cut(s) 4, 133, 317
LmnI GCTCC 2 cut(s) 45, 59
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 1 cut(s) 103
MaeI CTAG 2 cut(s) 155, 315
MaeII ACGT 2 cut(s) 160, 204
MaeIII GTNAC 2 cut(s) 102, 209
MalI GATC 3 cut(s) 6, 135, 319
MbiI CCGCTC 1 cut(s) 48
MboI GATC 3 cut(s) 4, 133, 317
MflI RGATCY 1 cut(s) 317
MluCI AATT 3 cut(s) 336, 349, 355
MlyI GAGTC 1 cut(s) 31
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 5 cut(s) 3, 44, 108, 119, 243
MseI TTAA 2 cut(s) 353, 397
MslI CAYNNNNRTG 1 cut(s) 40
MspA1I CMGCKG 2 cut(s) 177, 276
MspI CCGG 2 cut(s) 77, 329
MspR9I CCNGG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 5 cut(s) 66, 171, 174, 245, 282
NdeII GATC 3 cut(s) 4, 133, 317
NlaIII CATG 2 cut(s) 239, 380
NlaIV GGNNCC 1 cut(s) 55
NmeAIII GCCGAG 2 cut(s) 205, 286
NmuCI GTSAC 1 cut(s) 102
NsbI TGCGCA 1 cut(s) 181
OliI CACNNNNGTG 1 cut(s) 40
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 201
PfeI GAWTC 3 cut(s) 129, 216, 373
PkrI GCNGC 3 cut(s) 167, 176, 179
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 64
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 1 cut(s) 55
PsuI RGATCY 1 cut(s) 317
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
RseI CAYNNNNRTG 1 cut(s) 40
SaqAI TTAA 2 cut(s) 353, 397
SatI GCNGC 3 cut(s) 166, 175, 178
Sau3AI GATC 3 cut(s) 4, 133, 317
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 66
SetI ASST 3 cut(s) 163, 207, 235
SfaNI GCATC 1 cut(s) 103
SmiI ATTTAAAT 1 cut(s) 354
SmiMI CAYNNNNRTG 1 cut(s) 40
Sse9I AATT 3 cut(s) 336, 349, 355
SsiI CCGC 6 cut(s) 48, 120, 166, 175, 276, 332
SspMI CTAG 2 cut(s) 155, 315
StyD4I CCNGG 1 cut(s) 64
SwaI ATTTAAAT 1 cut(s) 354
TaaI ACNGT 2 cut(s) 143, 243
TaiI ACGT 2 cut(s) 163, 207
TaqI TCGA 2 cut(s) 132, 299
TasI AATT 3 cut(s) 336, 349, 355
TatI WGTACW 1 cut(s) 30
TauI GCSGC 2 cut(s) 168, 177
TfiI GAWTC 3 cut(s) 129, 216, 373
Tru1I TTAA 2 cut(s) 353, 397
Tru9I TTAA 2 cut(s) 353, 397
TscAI CASTG 3 cut(s) 33, 47, 130
TseFI GTSAC 1 cut(s) 102
TseI GCWGC 1 cut(s) 177
Tsp45I GTSAC 1 cut(s) 102
TspRI CASTG 3 cut(s) 33, 47, 130
XspI CTAG 2 cut(s) 155, 315
ZraI GACGTC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.