Rh4CG180700

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0017575
Physical Location & Seq
Forward (+)
39158530 .. 39158736
207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG180700.1

Sequence Viewer

Length: 207 bp
ATGTACTCGCACCAGTGGAGCGGAGGTTCGAGCAGCCCGGGCTCATCTCCGGCGATGTCGCCGGCGCATCCTCCGTCATGGCTCAATCCTTCCGCGACTGGATTCTCCACCGTCAAGCGGAACCAGAACCTCACCGCCAAAGCCGCCGCGCAGCGGCTTGCGCAAGTCATGGCTTCGCAGACGACGGTTGATGACGACGATCTCTGA

Protein Analysis

68

Amino Acids

7.13

Weight (kDa)

6.7

Isoelectric Point (pI)

68.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017575)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14570 FvH4_3g24840
malus_domestica MD06G1132800.v1.1
rosa_laevigata RLG00000008407 RLG00000016476 RLG00000022590
rosa_multiflora Rmu_sc0003990.1_g000002
rosa_samantha Rh1CG261300 Rh4CG007400 Rh4CG180700 Rh5AG034800 Rh5CG037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 162
AccBSI CCGCTC 1 cut(s) 21
AccII CGCG 2 cut(s) 95, 149
AciI CCGC 7 cut(s) 21, 93, 118, 135, 144, 147, 154
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 117, 153
AloI GAACNNNNNNTCC 2 cut(s) 10, 42
Ama87I CYCGRG 1 cut(s) 37
ApeKI GCWGC 2 cut(s) 33, 151
AspLEI GCGC 3 cut(s) 67, 151, 163
AsuC2I CCSGG 2 cut(s) 38, 39
AsuHPI GGTGA 1 cut(s) 124
AvaI CYCGRG 1 cut(s) 37
BanII GRGCYC 1 cut(s) 44
BbvI GCAGC 2 cut(s) 45, 163
BcnI CCSGG 2 cut(s) 38, 39
BisI GCNGC 5 cut(s) 34, 144, 147, 152, 155
BlsI GCNGC 5 cut(s) 35, 145, 148, 153, 156
Bme1390I CCNGG 2 cut(s) 38, 39
BmeT110I CYCGRG 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 122
BmrFI CCNGG 2 cut(s) 38, 39
BmsI GCATC 1 cut(s) 76
BpuMI CCSGG 2 cut(s) 38, 39
BsaJI CCNNGG 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 2 cut(s) 117, 153
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 2 cut(s) 13, 103
BseDI CCNNGG 1 cut(s) 37
BseGI GGATG 1 cut(s) 67
BseLI CCNNNNNNNGG 2 cut(s) 117, 153
BseNI ACTGG 2 cut(s) 13, 103
BseXI GCAGC 2 cut(s) 45, 163
Bsh1236I CGCG 2 cut(s) 95, 149
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 3 cut(s) 38, 50, 62
BslI CCNNNNNNNGG 2 cut(s) 117, 153
BsoBI CYCGRG 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 1 cut(s) 199
BspACI CCGC 7 cut(s) 21, 93, 118, 135, 144, 147, 154
BspFNI CGCG 2 cut(s) 95, 149
BspLI GGNNCC 1 cut(s) 122
BsrBI CCGCTC 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 2 cut(s) 13, 103
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 1 cut(s) 199
Bst4CI ACNGT 2 cut(s) 112, 187
BstC8I GCNNGC 2 cut(s) 63, 159
BstF5I GGATG 1 cut(s) 67
BstFNI CGCG 2 cut(s) 95, 149
BstHHI GCGC 3 cut(s) 67, 151, 163
BstKTI GATC 1 cut(s) 202
BstMBI GATC 1 cut(s) 199
BstMWI GCNNNNNNNGC 3 cut(s) 39, 143, 160
BstSCI CCNGG 2 cut(s) 36, 37
BstUI CGCG 2 cut(s) 95, 149
BstV1I GCAGC 2 cut(s) 45, 163
BtgZI GCGATG 1 cut(s) 68
BtsCI GGATG 1 cut(s) 67
BtsIMutI CAGTG 1 cut(s) 20
Cac8I GCNNGC 2 cut(s) 63, 159
CfoI GCGC 3 cut(s) 67, 151, 163
Cfr10I RCCGGY 1 cut(s) 61
Cfr9I CCCGGG 1 cut(s) 37
Csp6I GTAC 1 cut(s) 4
CviAII CATG 2 cut(s) 78, 169
CviJI RGCY 6 cut(s) 36, 42, 82, 143, 157, 173
CviKI_1 RGCY 6 cut(s) 36, 42, 82, 143, 157, 173
CviQI GTAC 1 cut(s) 4
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 37
EcoT38I GRGCYC 1 cut(s) 44
FaeI CATG 2 cut(s) 81, 172
FaiI YATR 2 cut(s) 79, 170
FatI CATG 2 cut(s) 77, 168
Fnu4HI GCNGC 5 cut(s) 34, 144, 147, 152, 155
FokI GGATG 1 cut(s) 54
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 5 cut(s) 34, 144, 147, 152, 155
FspI TGCGCA 1 cut(s) 162
GlaI GCGC 3 cut(s) 66, 150, 162
GluI GCNGC 5 cut(s) 34, 144, 147, 152, 155
HapII CCGG 3 cut(s) 38, 50, 62
HhaI GCGC 3 cut(s) 67, 151, 163
Hin1II CATG 2 cut(s) 81, 172
Hin6I GCGC 3 cut(s) 65, 149, 161
HinP1I GCGC 3 cut(s) 65, 149, 161
HinfI GANTC 1 cut(s) 102
HpaII CCGG 3 cut(s) 38, 50, 62
HphI GGTGA 1 cut(s) 124
Hpy188I TCNGA 1 cut(s) 206
Hpy99I CGWCG 2 cut(s) 187, 200
HpyAV CCTTC 1 cut(s) 99
HpyCH4III ACNGT 2 cut(s) 112, 187
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 143, 160
Hsp92II CATG 2 cut(s) 81, 172
HspAI GCGC 3 cut(s) 65, 149, 161
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Kzo9I GATC 1 cut(s) 199
LmnI GCTCC 1 cut(s) 18
LpnPI CCDG 6 cut(s) 26, 51, 63, 75, 84, 137
Lsp1109I GCAGC 2 cut(s) 45, 163
LweI GCATC 1 cut(s) 76
MalI GATC 1 cut(s) 201
MbiI CCGCTC 1 cut(s) 21
MboI GATC 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 44
MnlI CCTC 3 cut(s) 17, 81, 140
MreI CGCCGGCG 1 cut(s) 61
MroNI GCCGGC 1 cut(s) 61
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 3 cut(s) 38, 50, 62
MspR9I CCNGG 2 cut(s) 38, 39
MvnI CGCG 2 cut(s) 95, 149
MwoI GCNNNNNNNGC 3 cut(s) 39, 143, 160
NaeI GCCGGC 1 cut(s) 63
NciI CCSGG 2 cut(s) 38, 39
NdeII GATC 1 cut(s) 199
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 2 cut(s) 81, 172
NlaIV GGNNCC 1 cut(s) 122
NsbI TGCGCA 1 cut(s) 162
PdiI GCCGGC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 102
PkrI GCNGC 5 cut(s) 35, 145, 148, 153, 156
PspN4I GGNNCC 1 cut(s) 122
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SatI GCNGC 5 cut(s) 34, 144, 147, 152, 155
Sau3AI GATC 1 cut(s) 199
ScrFI CCNGG 2 cut(s) 38, 39
SduI GDGCHC 1 cut(s) 44
SetI ASST 2 cut(s) 28, 132
SfaNI GCATC 1 cut(s) 76
SgrAI CRCCGGYG 1 cut(s) 61
SmaI CCCGGG 1 cut(s) 39
SrfI GCCCGGGC 1 cut(s) 39
SsiI CCGC 7 cut(s) 21, 93, 118, 135, 144, 147, 154
StyD4I CCNGG 2 cut(s) 36, 37
TaaI ACNGT 2 cut(s) 112, 187
TaqI TCGA 1 cut(s) 29
TatI WGTACW 1 cut(s) 3
TauI GCSGC 3 cut(s) 146, 149, 157
TfiI GAWTC 1 cut(s) 102
TscAI CASTG 1 cut(s) 20
TseI GCWGC 2 cut(s) 33, 151
TspGWI ACGGA 1 cut(s) 63
TspMI CCCGGG 1 cut(s) 37
TspRI CASTG 1 cut(s) 20
XmaI CCCGGG 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.