RLG00000016476

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017575
Physical Location & Seq
Reverse (-)
6980260 .. 6980651
392 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016476

Sequence Viewer

Length: 357 bp
ATGTACTCGCACCAGTGGAGTGGAGGTTCGAGCAGCCCGGGCTCATCTCCGGCGATGTCGCCGGCACATCCTCCATCATGGCTCAATCCTTCCGCGACTGGATTCTCCACTGTCAAGCGGAACCAGAACCTCACCGCCAAAGCCGCCGCGCAGCGGCTTGGGCAAATCATGGCTTCGCAGACGACGCTAGCAGCCTCATGCGCCGTCTTCCCCTCGATTCCTCTTCTTCGCTCGATTCCGTTGTCGACGCTGACTTCAATGCCAACGAAGGCTTCTCCTCGCTCGATTCGATGTTGCAGTGAGCGTTGGGACTTCGATCTGAACAAGCAACGGCTCGAGATAAAGCAGCTCCGGTGA

Protein Analysis

119

Amino Acids

12.79

Weight (kDa)

10.59

Isoelectric Point (pI)

82.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017575)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14570 FvH4_3g24840
malus_domestica MD06G1132800.v1.1
rosa_laevigata RLG00000008407 RLG00000016476 RLG00000022590
rosa_multiflora Rmu_sc0003990.1_g000002
rosa_samantha Rh1CG261300 Rh4CG007400 Rh4CG180700 Rh5AG034800 Rh5CG037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 245
AccII CGCG 2 cut(s) 95, 149
AciI CCGC 6 cut(s) 93, 118, 135, 144, 147, 154
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 153
AgsI TTSAA 1 cut(s) 258
AjuI GAANNNNNNNTTGG 2 cut(s) 256, 288
AloI GAACNNNNNNTCC 2 cut(s) 10, 42
AluBI AGCT 1 cut(s) 349
AluI AGCT 1 cut(s) 349
Ama87I CYCGRG 2 cut(s) 37, 335
ApeKI GCWGC 4 cut(s) 33, 151, 191, 346
AspLEI GCGC 2 cut(s) 151, 203
AsuC2I CCSGG 2 cut(s) 38, 39
AsuHPI GGTGA 1 cut(s) 124
AsuNHI GCTAGC 1 cut(s) 187
AvaI CYCGRG 2 cut(s) 37, 335
BanII GRGCYC 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 199
BbvI GCAGC 3 cut(s) 45, 163, 203
BccI CCATC 1 cut(s) 82
BceAI ACGGC 2 cut(s) 188, 347
BcnI CCSGG 2 cut(s) 38, 39
BfaI CTAG 1 cut(s) 188
BisI GCNGC 7 cut(s) 34, 144, 147, 152, 155, 192, 347
BlsI GCNGC 7 cut(s) 35, 145, 148, 153, 156, 193, 348
Bme1390I CCNGG 2 cut(s) 38, 39
BmeT110I CYCGRG 2 cut(s) 37, 335
BmiI GGNNCC 1 cut(s) 122
BmrFI CCNGG 2 cut(s) 38, 39
BmtI GCTAGC 1 cut(s) 191
BpiI GAAGAC 1 cut(s) 199
BpuMI CCSGG 2 cut(s) 38, 39
BsaJI CCNNGG 1 cut(s) 37
BsaWI WCCGGW 1 cut(s) 351
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 1 cut(s) 153
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 2 cut(s) 13, 103
BseDI CCNNGG 1 cut(s) 37
BseGI GGATG 1 cut(s) 67
BseLI CCNNNNNNNGG 1 cut(s) 153
BseNI ACTGG 2 cut(s) 13, 103
BseRI GAGGAG 1 cut(s) 267
BseXI GCAGC 3 cut(s) 45, 163, 203
Bsh1236I CGCG 2 cut(s) 95, 149
BsiHKCI CYCGRG 2 cut(s) 37, 335
BsiSI CCGG 4 cut(s) 38, 50, 62, 352
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 323
BsoBI CYCGRG 2 cut(s) 37, 335
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 1 cut(s) 316
BspACI CCGC 6 cut(s) 93, 118, 135, 144, 147, 154
BspFNI CGCG 2 cut(s) 95, 149
BspLI GGNNCC 1 cut(s) 122
BspOI GCTAGC 1 cut(s) 191
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 2 cut(s) 13, 103
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 1 cut(s) 316
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 228
BstC8I GCNNGC 2 cut(s) 63, 189
BstF5I GGATG 1 cut(s) 67
BstFNI CGCG 2 cut(s) 95, 149
BstHHI GCGC 2 cut(s) 151, 203
BstKTI GATC 1 cut(s) 319
BstMBI GATC 1 cut(s) 316
BstMWI GCNNNNNNNGC 5 cut(s) 39, 143, 160, 184, 200
BstSCI CCNGG 2 cut(s) 36, 37
BstUI CGCG 2 cut(s) 95, 149
BstV1I GCAGC 3 cut(s) 45, 163, 203
BstV2I GAAGAC 1 cut(s) 199
BstXI CCANNNNNNTGG 1 cut(s) 20
BtgZI GCGATG 1 cut(s) 68
BtsCI GGATG 1 cut(s) 67
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 3 cut(s) 20, 108, 304
Cac8I GCNNGC 2 cut(s) 63, 189
CfoI GCGC 2 cut(s) 151, 203
Cfr10I RCCGGY 1 cut(s) 61
Cfr9I CCCGGG 1 cut(s) 37
CseI GACGC 2 cut(s) 193, 256
Csp6I GTAC 1 cut(s) 4
CviAII CATG 3 cut(s) 78, 169, 198
CviQI GTAC 1 cut(s) 4
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 44
Eco88I CYCGRG 2 cut(s) 37, 335
EcoT38I GRGCYC 1 cut(s) 44
FaeI CATG 3 cut(s) 81, 172, 201
FaiI YATR 3 cut(s) 79, 170, 199
FaqI GGGAC 1 cut(s) 323
FatI CATG 3 cut(s) 77, 168, 197
FblI GTMKAC 1 cut(s) 245
Fnu4HI GCNGC 7 cut(s) 34, 144, 147, 152, 155, 192, 347
FokI GGATG 1 cut(s) 54
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 7 cut(s) 34, 144, 147, 152, 155, 192, 347
FspBI CTAG 1 cut(s) 188
GlaI GCGC 2 cut(s) 150, 202
GluI GCNGC 7 cut(s) 34, 144, 147, 152, 155, 192, 347
HapII CCGG 4 cut(s) 38, 50, 62, 352
HgaI GACGC 2 cut(s) 193, 256
HhaI GCGC 2 cut(s) 151, 203
Hin1II CATG 3 cut(s) 81, 172, 201
Hin6I GCGC 2 cut(s) 149, 201
HinP1I GCGC 2 cut(s) 149, 201
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HinfI GANTC 4 cut(s) 102, 217, 235, 286
HpaII CCGG 4 cut(s) 38, 50, 62, 352
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 1 cut(s) 321
Hpy188III TCNNGA 1 cut(s) 337
Hpy8I GTNNAC 1 cut(s) 246
Hpy99I CGWCG 2 cut(s) 187, 250
HpyAV CCTTC 2 cut(s) 99, 262
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4V TGCA 1 cut(s) 297
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 143, 160, 184, 200
Hsp92II CATG 3 cut(s) 81, 172, 201
HspAI GCGC 2 cut(s) 149, 201
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Kzo9I GATC 1 cut(s) 316
LmnI GCTCC 1 cut(s) 354
LpnPI CCDG 6 cut(s) 26, 51, 63, 75, 84, 137
Lsp1109I GCAGC 3 cut(s) 45, 163, 203
MaeI CTAG 1 cut(s) 188
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 3 cut(s) 199, 215, 218
MhlI GDGCHC 1 cut(s) 44
MnlI CCTC 7 cut(s) 17, 81, 140, 205, 223, 231, 288
MroNI GCCGGC 1 cut(s) 61
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 4 cut(s) 38, 50, 62, 352
MspR9I CCNGG 2 cut(s) 38, 39
MvnI CGCG 2 cut(s) 95, 149
MwoI GCNNNNNNNGC 5 cut(s) 39, 143, 160, 184, 200
NaeI GCCGGC 1 cut(s) 63
NciI CCSGG 2 cut(s) 38, 39
NdeII GATC 1 cut(s) 316
NgoMIV GCCGGC 1 cut(s) 61
NheI GCTAGC 1 cut(s) 187
NlaIII CATG 3 cut(s) 81, 172, 201
NlaIV GGNNCC 1 cut(s) 122
PaeR7I CTCGAG 1 cut(s) 335
PcsI WCGNNNNNNNCGW 1 cut(s) 286
PdiI GCCGGC 1 cut(s) 63
PfeI GAWTC 4 cut(s) 102, 217, 235, 286
PkrI GCNGC 7 cut(s) 35, 145, 148, 153, 156, 193, 348
PspN4I GGNNCC 1 cut(s) 122
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SalI GTCGAC 1 cut(s) 244
SatI GCNGC 7 cut(s) 34, 144, 147, 152, 155, 192, 347
Sau3AI GATC 1 cut(s) 316
ScrFI CCNGG 2 cut(s) 38, 39
SduI GDGCHC 1 cut(s) 44
SetI ASST 3 cut(s) 28, 132, 351
Sfr274I CTCGAG 1 cut(s) 335
SlaI CTCGAG 1 cut(s) 335
SmaI CCCGGG 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
SrfI GCCCGGGC 1 cut(s) 39
SsiI CCGC 6 cut(s) 93, 118, 135, 144, 147, 154
SspMI CTAG 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 36, 37
TaaI ACNGT 1 cut(s) 112
TaqI TCGA 8 cut(s) 29, 215, 233, 245, 284, 289, 315, 336
TatI WGTACW 1 cut(s) 3
TauI GCSGC 3 cut(s) 146, 149, 157
TfiI GAWTC 4 cut(s) 102, 217, 235, 286
TscAI CASTG 3 cut(s) 20, 115, 304
TseI GCWGC 4 cut(s) 33, 151, 191, 346
TspGWI ACGGA 1 cut(s) 228
TspMI CCCGGG 1 cut(s) 37
TspRI CASTG 3 cut(s) 20, 115, 304
XhoI CTCGAG 1 cut(s) 335
XmaI CCCGGG 1 cut(s) 37
XmiI GTMKAC 1 cut(s) 245
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.