FvH4_3g25140

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016153
Physical Location & Seq
Reverse (-)
18150120 .. 18150674
555 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25140.t1

Sequence Viewer

Length: 555 bp
ATGAGCCGCAGGGAATCGAAATGGAGCTGGACCCACGCCCTCGTCGGCGCCGCTTCCGCCACAGCCGCCTTCGGCCTCCTCTCCGCCAAACCCAAAGATCCGACCTTCGACCTCATTTCCATCGACCTCACCTCCTTCAAGCTCAACCTCCCTCTCCTCGACGCCGACCTCATCCTCACCGTCCACGTCACCAACCCCAACGTGGTCCCCATCCACTACTCCTCCTCCACCATGTCCATCTTCTACAAAGGCTCCCTCCTCGGCTCCGCTGACGTCAAGGCCGGCTCTCAGTCCGCCAACTCCTGCAAGCTCCTCCGCCTCCCCGCCCGCCTCGACGGCCTCCAGCTCGCCCACCACGCCGCCAGCTTCGTCGCCGACGTCGCCAAGAGGGAGATGGTGCTCGACGCCGCCGTCGATATCGCCGGTGCCGCCAAGGTTCTGTGGTGGGATCACAAGTTCGTGGTGCACGTGGACAGTCACGTGACAGTTGATCCTCTCTTTCTTGATGTCGTTGAGCAGGAAAACAAGTCGGAGTTGGATCTCTTCGTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

185

Amino Acids

19.98

Weight (kDa)

6.02

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 60 - 157 1.1e-11 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44380
fragaria_vesca FvH4_3g25140 FvH4_3g25160
malus_domestica MD03G1147800.v1.1 MD11G1166600.v1.1
prunus_persica Prupe.6G132600_v2.0.a1 Prupe.6G132700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0046191 RchiOBHm_Chr5g0046241
rosa_laevigata RLG00000034378
rosa_multiflora Rmu_sc0017875.1_g000001
rosa_roxburghii Rroxscaffold_1G00035440
rosa_rugosa Rorug05G0224500
rosa_samantha Rh5DG324600
rosa_wichuraiana Rw5G028600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 410
AatII GACGTC 2 cut(s) 276, 381
AccB1I GGYRCC 2 cut(s) 47, 425
AclWI GGATC 4 cut(s) 92, 456, 485, 546
AcvI CACGTG 2 cut(s) 469, 481
AcyI GRCGYC 5 cut(s) 48, 162, 273, 378, 405
AfiI CCNNNNNNNGG 1 cut(s) 202
AgsI TTSAA 1 cut(s) 139
AjiI CACGTC 1 cut(s) 187
AloI GAACNNNNNNTCC 2 cut(s) 440, 472
AluBI AGCT 5 cut(s) 27, 142, 310, 346, 366
AluI AGCT 5 cut(s) 27, 142, 310, 346, 366
Alw21I GWGCWC 2 cut(s) 402, 468
Alw44I GTGCAC 1 cut(s) 464
AlwI GGATC 4 cut(s) 92, 456, 485, 546
AoxI GGCC 3 cut(s) 73, 279, 337
ApaLI GTGCAC 1 cut(s) 464
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 2 cut(s) 30, 205
AsuHPI GGTGA 3 cut(s) 121, 169, 181
AvaII GGWCC 2 cut(s) 30, 205
BaeGI GKGCMC 1 cut(s) 468
BanI GGYRCC 2 cut(s) 47, 425
BbrPI CACGTG 2 cut(s) 469, 481
Bbv12I GWGCWC 2 cut(s) 402, 468
BccI CCATC 4 cut(s) 128, 218, 245, 388
BceAI ACGGC 2 cut(s) 352, 395
BfoI RGCGCY 1 cut(s) 51
BglI GCCNNNNNGGC 1 cut(s) 336
BisI GCNGC 6 cut(s) 7, 51, 66, 360, 408, 429
BlsI GCNGC 6 cut(s) 8, 52, 67, 361, 409, 430
Bme18I GGWCC 2 cut(s) 30, 205
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 30, 205
BmiI GGNNCC 6 cut(s) 32, 49, 207, 253, 265, 427
BpmI CTGGAG 1 cut(s) 326
BsaAI YACGTR 2 cut(s) 469, 481
BsaHI GRCGYC 5 cut(s) 48, 162, 273, 378, 405
BsaJI CCNNGG 2 cut(s) 259, 432
BsaXI ACNNNNNCTCC 8 cut(s) 116, 146, 206, 209, 236, 236, 239, 266
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse118I RCCGGY 2 cut(s) 281, 422
BseDI CCNNGG 2 cut(s) 259, 432
BseGI GGATG 2 cut(s) 171, 210
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMII CTCAG 1 cut(s) 302
BseRI GAGGAG 6 cut(s) 68, 146, 211, 214, 248, 302
BseSI GKGCMC 1 cut(s) 468
BshFI GGCC 3 cut(s) 75, 281, 339
BshNI GGYRCC 2 cut(s) 47, 425
BsiHKAI GWGCWC 2 cut(s) 402, 468
BsiSI CCGG 2 cut(s) 282, 423
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 202
BsmFI GGGAC 1 cut(s) 191
BsnI GGCC 3 cut(s) 75, 281, 339
Bsp1286I GDGCHC 2 cut(s) 402, 468
Bsp143I GATC 4 cut(s) 97, 448, 490, 538
BspANI GGCC 3 cut(s) 75, 281, 339
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 6 cut(s) 32, 49, 207, 253, 265, 427
BspPI GGATC 4 cut(s) 92, 456, 485, 546
BspT107I GGYRCC 2 cut(s) 47, 425
BsrFI RCCGGY 2 cut(s) 281, 422
BssAI RCCGGY 2 cut(s) 281, 422
BssECI CCNNGG 2 cut(s) 259, 432
BssMI GATC 4 cut(s) 97, 448, 490, 538
BssNI GRCGYC 5 cut(s) 48, 162, 273, 378, 405
BssT1I CCWWGG 1 cut(s) 432
Bst4CI ACNGT 3 cut(s) 181, 476, 487
Bst6I CTCTTC 1 cut(s) 548
BstACI GRCGYC 5 cut(s) 48, 162, 273, 378, 405
BstBAI YACGTR 2 cut(s) 469, 481
BstC8I GCNNGC 5 cut(s) 283, 308, 328, 348, 364
BstDEI CTNAG 1 cut(s) 288
BstF5I GGATG 2 cut(s) 171, 210
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 4 cut(s) 100, 451, 493, 541
BstMBI GATC 4 cut(s) 97, 448, 490, 538
BstMWI GCNNNNNNNGC 6 cut(s) 56, 65, 336, 356, 380, 428
BstSLI GKGCMC 1 cut(s) 468
BstX2I RGATCY 2 cut(s) 97, 538
BstYI RGATCY 2 cut(s) 97, 538
BsuRI GGCC 3 cut(s) 75, 281, 339
BtrI CACGTC 1 cut(s) 187
BtsCI GGATG 2 cut(s) 171, 210
Cac8I GCNNGC 5 cut(s) 283, 308, 328, 348, 364
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 2 cut(s) 281, 422
Cfr13I GGNCC 2 cut(s) 30, 205
CseI GACGC 2 cut(s) 170, 413
CviAII CATG 1 cut(s) 232
DdeI CTNAG 1 cut(s) 288
DinI GGCGCC 1 cut(s) 49
DpnI GATC 4 cut(s) 99, 450, 492, 540
DpnII GATC 4 cut(s) 97, 448, 490, 538
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
Eam1104I CTCTTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 548
EciI GGCGGA 4 cut(s) 46, 73, 283, 305
Eco130I CCWWGG 1 cut(s) 432
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 2 cut(s) 30, 205
Eco72I CACGTG 2 cut(s) 469, 481
EcoRV GATATC 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 432
EgeI GGCGCC 1 cut(s) 49
EheI GGCGCC 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 1 cut(s) 235
FaiI YATR 1 cut(s) 233
FaqI GGGAC 1 cut(s) 191
FatI CATG 1 cut(s) 231
FauI CCCGC 2 cut(s) 331, 335
Fnu4HI GCNGC 6 cut(s) 7, 51, 66, 360, 408, 429
FokI GGATG 2 cut(s) 158, 197
Fsp4HI GCNGC 6 cut(s) 7, 51, 66, 360, 408, 429
GlaI GCGC 1 cut(s) 49
GluI GCNGC 6 cut(s) 7, 51, 66, 360, 408, 429
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 3 cut(s) 75, 281, 339
HapII CCGG 2 cut(s) 282, 423
HgaI GACGC 2 cut(s) 170, 413
HhaI GCGC 1 cut(s) 50
Hin1I GRCGYC 5 cut(s) 48, 162, 273, 378, 405
Hin1II CATG 1 cut(s) 235
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HinfI GANTC 1 cut(s) 14
HpaII CCGG 2 cut(s) 282, 423
HphI GGTGA 3 cut(s) 121, 169, 181
Hpy166II GTNNAC 3 cut(s) 184, 466, 472
Hpy188I TCNGA 2 cut(s) 102, 532
Hpy188III TCNNGA 1 cut(s) 503
Hpy8I GTNNAC 3 cut(s) 184, 466, 472
Hpy99I CGWCG 8 cut(s) 47, 164, 338, 374, 380, 383, 407, 416
HpyAV CCTTC 3 cut(s) 79, 115, 145
HpyCH4III ACNGT 3 cut(s) 181, 476, 487
HpyCH4IV ACGT 6 cut(s) 186, 201, 273, 378, 468, 480
HpyCH4V TGCA 2 cut(s) 306, 466
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 65, 336, 356, 380, 428
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 6 cut(s) 186, 201, 273, 378, 468, 480
Hsp92I GRCGYC 5 cut(s) 48, 162, 273, 378, 405
Hsp92II CATG 1 cut(s) 235
HspAI GCGC 1 cut(s) 48
KasI GGCGCC 1 cut(s) 47
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
Kzo9I GATC 4 cut(s) 97, 448, 490, 538
LmnI GCTCC 4 cut(s) 24, 257, 269, 315
LpnPI CCDG 7 cut(s) 13, 295, 316, 356, 376, 436, 503
MaeII ACGT 6 cut(s) 186, 201, 273, 378, 468, 480
MaeIII GTNAC 3 cut(s) 187, 476, 481
MalI GATC 4 cut(s) 99, 450, 492, 540
MboI GATC 4 cut(s) 97, 448, 490, 538
MboII GAAGA 2 cut(s) 232, 535
MflI RGATCY 2 cut(s) 97, 538
MhlI GDGCHC 2 cut(s) 402, 468
Mly113I GGCGCC 1 cut(s) 48
MmeI TCCRAC 3 cut(s) 125, 510, 516
MroNI GCCGGC 1 cut(s) 281
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 2 cut(s) 282, 423
MwoI GCNNNNNNNGC 6 cut(s) 56, 65, 336, 356, 380, 428
NaeI GCCGGC 1 cut(s) 283
NarI GGCGCC 1 cut(s) 48
NdeII GATC 4 cut(s) 97, 448, 490, 538
NgoMIV GCCGGC 1 cut(s) 281
NlaIII CATG 1 cut(s) 235
NlaIV GGNNCC 6 cut(s) 32, 49, 207, 253, 265, 427
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 3 cut(s) 187, 476, 481
PcsI WCGNNNNNNNCGW 4 cut(s) 375, 408, 411, 465
PdiI GCCGGC 1 cut(s) 283
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 6 cut(s) 8, 52, 67, 361, 409, 430
PluTI GGCGCC 1 cut(s) 51
PmaCI CACGTG 2 cut(s) 469, 481
PmlI CACGTG 2 cut(s) 469, 481
Ppu21I YACGTR 2 cut(s) 469, 481
PspCI CACGTG 2 cut(s) 469, 481
PspN4I GGNNCC 6 cut(s) 32, 49, 207, 253, 265, 427
PspPI GGNCC 2 cut(s) 30, 205
PsuI RGATCY 2 cut(s) 97, 538
SatI GCNGC 6 cut(s) 7, 51, 66, 360, 408, 429
Sau3AI GATC 4 cut(s) 97, 448, 490, 538
Sau96I GGNCC 2 cut(s) 30, 205
SduI GDGCHC 2 cut(s) 402, 468
SfoI GGCGCC 1 cut(s) 49
SgrAI CRCCGGYG 1 cut(s) 422
SinI GGWCC 2 cut(s) 30, 205
SspDI GGCGCC 1 cut(s) 47
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 3 cut(s) 181, 476, 487
TaiI ACGT 6 cut(s) 189, 204, 276, 381, 471, 483
TaqI TCGA 7 cut(s) 17, 108, 123, 159, 333, 402, 414
TauI GCSGC 6 cut(s) 9, 53, 68, 362, 410, 431
TfiI GAWTC 1 cut(s) 14
TseFI GTSAC 3 cut(s) 187, 476, 481
Tsp45I GTSAC 3 cut(s) 187, 476, 481
VneI GTGCAC 1 cut(s) 464
VpaK11BI GGWCC 2 cut(s) 30, 205
XcmI CCANNNNNNNNNTGG 2 cut(s) 199, 391
ZraI GACGTC 2 cut(s) 274, 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.