FvH4_3g25160

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016153
Physical Location & Seq
Reverse (-)
18154193 .. 18154747
555 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25160.t1

Sequence Viewer

Length: 555 bp
ATGAGCCGCACGGAAGATAATTGGTTCTTGTCCTCCTCCCTATTCGGCGCCGCTTCCGCCGTCGTCATCACCGGCCTCCTCGTCGCGAAGCCCAAGGACCCGACCTTCGACCTCGTCTCCATCGACCTCACCTCCTTCAAGTTCAACCTCCCTCTCCTCGACGTCGGCCTCGTCCTCACCGTCAACATCACCAACCCCAACATCGTGCCCATCCACTACTCCTCCTCCACCATGTCCATCTTCTACAAAGGCGCCCTCCTCGGCTCTGCTGACATCAAGGCCGGCTCCCAGTCCGCCAAGTCCTCCAAGGTCCTCCGCCTCCCCGCTCGTCTCAACGGCCTCCAGCTCGCCCACCACGCCGCCAGCTTCGTCGCCGACGTCGCCAAGAGGGAGATGGTGCTCGACGCCGCCGTTGATATCGCCGGTGCCGCCAAGGTTCTGTGGTGGGATCACAAGTTCGCGGTCCACGTCGACAGTCATTTGACCGTTGATCCTTTGTTTCTCGACGTCGTTGAGCAGGAAAACAAGTCGGAGTTGGATCTCTTTGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

185

Amino Acids

19.89

Weight (kDa)

5.8

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 60 - 157 7.2e-13 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44380
fragaria_vesca FvH4_3g25140 FvH4_3g25160
malus_domestica MD03G1147800.v1.1 MD11G1166600.v1.1
prunus_persica Prupe.6G132600_v2.0.a1 Prupe.6G132700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0046191 RchiOBHm_Chr5g0046241
rosa_laevigata RLG00000034378
rosa_multiflora Rmu_sc0017875.1_g000001
rosa_roxburghii Rroxscaffold_1G00035440
rosa_rugosa Rorug05G0224500
rosa_samantha Rh5DG324600
rosa_wichuraiana Rw5G028600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 165, 381, 510
AccB1I GGYRCC 3 cut(s) 47, 251, 425
AccBSI CCGCTC 1 cut(s) 326
AccI GTMKAC 1 cut(s) 471
AccII CGCG 2 cut(s) 86, 461
AclWI GGATC 3 cut(s) 456, 485, 546
AcyI GRCGYC 6 cut(s) 48, 162, 252, 378, 405, 507
AgsI TTSAA 2 cut(s) 139, 145
AjiI CACGTC 1 cut(s) 469
AloI GAACNNNNNNTCC 2 cut(s) 440, 472
AluBI AGCT 2 cut(s) 346, 366
AluI AGCT 2 cut(s) 346, 366
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 2 cut(s) 121, 335
AlwI GGATC 3 cut(s) 456, 485, 546
AoxI GGCC 4 cut(s) 73, 166, 279, 337
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AspLEI GCGC 2 cut(s) 50, 254
AspS9I GGNCC 3 cut(s) 97, 310, 463
AsuHPI GGTGA 4 cut(s) 61, 121, 169, 181
AvaII GGWCC 3 cut(s) 97, 310, 463
BaeGI GKGCMC 1 cut(s) 210
BanI GGYRCC 3 cut(s) 47, 251, 425
Bbv12I GWGCWC 1 cut(s) 402
BccI CCATC 4 cut(s) 128, 218, 245, 388
BceAI ACGGC 3 cut(s) 44, 352, 395
BcoDI GTCTC 2 cut(s) 121, 335
BfoI RGCGCY 2 cut(s) 51, 255
BisI GCNGC 5 cut(s) 7, 51, 360, 408, 429
BlsI GCNGC 5 cut(s) 8, 52, 361, 409, 430
Bme18I GGWCC 3 cut(s) 97, 310, 463
BmgBI CACGTC 1 cut(s) 469
BmgT120I GGNCC 3 cut(s) 97, 310, 463
BmiI GGNNCC 5 cut(s) 49, 99, 253, 286, 427
BmrI ACTGGG 1 cut(s) 283
BmuI ACTGGG 1 cut(s) 283
BpmI CTGGAG 1 cut(s) 326
BsaHI GRCGYC 6 cut(s) 48, 162, 252, 378, 405, 507
BsaJI CCNNGG 4 cut(s) 93, 259, 306, 432
BsaXI ACNNNNNCTCC 8 cut(s) 101, 116, 131, 146, 206, 209, 236, 239
Bse118I RCCGGY 3 cut(s) 71, 281, 422
Bse1I ACTGG 1 cut(s) 289
BseDI CCNNGG 4 cut(s) 93, 259, 306, 432
BseGI GGATG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 289
BseRI GAGGAG 6 cut(s) 25, 68, 146, 211, 214, 248
BseSI GKGCMC 1 cut(s) 210
Bsh1236I CGCG 2 cut(s) 86, 461
BshFI GGCC 4 cut(s) 75, 168, 281, 339
BshNI GGYRCC 3 cut(s) 47, 251, 425
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 3 cut(s) 72, 282, 423
BsmAI GTCTC 2 cut(s) 121, 335
BsmBI CGTCTC 2 cut(s) 121, 335
BsnI GGCC 4 cut(s) 75, 168, 281, 339
Bsp1286I GDGCHC 2 cut(s) 210, 402
Bsp143I GATC 3 cut(s) 448, 490, 538
Bsp68I TCGCGA 1 cut(s) 86
BspANI GGCC 4 cut(s) 75, 168, 281, 339
BspFNI CGCG 2 cut(s) 86, 461
BspLI GGNNCC 5 cut(s) 49, 99, 253, 286, 427
BspPI GGATC 3 cut(s) 456, 485, 546
BspT107I GGYRCC 3 cut(s) 47, 251, 425
BsrBI CCGCTC 1 cut(s) 326
BsrFI RCCGGY 3 cut(s) 71, 281, 422
BsrI ACTGG 1 cut(s) 289
BssAI RCCGGY 3 cut(s) 71, 281, 422
BssECI CCNNGG 4 cut(s) 93, 259, 306, 432
BssMI GATC 3 cut(s) 448, 490, 538
BssNI GRCGYC 6 cut(s) 48, 162, 252, 378, 405, 507
BssT1I CCWWGG 3 cut(s) 93, 306, 432
Bst4CI ACNGT 3 cut(s) 181, 476, 487
BstACI GRCGYC 6 cut(s) 48, 162, 252, 378, 405, 507
BstC8I GCNNGC 3 cut(s) 283, 348, 364
BstF5I GGATG 1 cut(s) 210
BstFNI CGCG 2 cut(s) 86, 461
BstH2I RGCGCY 2 cut(s) 51, 255
BstHHI GCGC 2 cut(s) 50, 254
BstKTI GATC 3 cut(s) 451, 493, 541
BstMAI GTCTC 2 cut(s) 121, 335
BstMBI GATC 3 cut(s) 448, 490, 538
BstMWI GCNNNNNNNGC 4 cut(s) 56, 356, 380, 428
BstSLI GKGCMC 1 cut(s) 210
BstUI CGCG 2 cut(s) 86, 461
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
BsuRI GGCC 4 cut(s) 75, 168, 281, 339
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 1 cut(s) 210
BtuMI TCGCGA 1 cut(s) 86
Cac8I GCNNGC 3 cut(s) 283, 348, 364
CfoI GCGC 2 cut(s) 50, 254
Cfr10I RCCGGY 3 cut(s) 71, 281, 422
Cfr13I GGNCC 3 cut(s) 97, 310, 463
CseI GACGC 1 cut(s) 413
CviAII CATG 1 cut(s) 232
DinI GGCGCC 2 cut(s) 49, 253
DpnI GATC 3 cut(s) 450, 492, 540
DpnII GATC 3 cut(s) 448, 490, 538
EciI GGCGGA 3 cut(s) 46, 283, 305
Eco130I CCWWGG 3 cut(s) 93, 306, 432
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 3 cut(s) 97, 310, 463
EcoO109I RGGNCCY 2 cut(s) 97, 310
EcoRV GATATC 1 cut(s) 418
EcoT14I CCWWGG 3 cut(s) 93, 306, 432
EgeI GGCGCC 2 cut(s) 49, 253
EheI GGCGCC 2 cut(s) 49, 253
ErhI CCWWGG 3 cut(s) 93, 306, 432
Esp3I CGTCTC 2 cut(s) 121, 335
FaeI CATG 1 cut(s) 235
FaiI YATR 1 cut(s) 233
FatI CATG 1 cut(s) 231
FauI CCCGC 1 cut(s) 331
FblI GTMKAC 1 cut(s) 471
Fnu4HI GCNGC 5 cut(s) 7, 51, 360, 408, 429
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 5 cut(s) 7, 51, 360, 408, 429
GlaI GCGC 2 cut(s) 49, 253
GluI GCNGC 5 cut(s) 7, 51, 360, 408, 429
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 2 cut(s) 51, 255
HaeIII GGCC 4 cut(s) 75, 168, 281, 339
HapII CCGG 3 cut(s) 72, 282, 423
HgaI GACGC 1 cut(s) 413
HhaI GCGC 2 cut(s) 50, 254
Hin1I GRCGYC 6 cut(s) 48, 162, 252, 378, 405, 507
Hin1II CATG 1 cut(s) 235
Hin6I GCGC 2 cut(s) 48, 252
HinP1I GCGC 2 cut(s) 48, 252
HincII GTYRAC 2 cut(s) 184, 472
HindII GTYRAC 2 cut(s) 184, 472
HpaII CCGG 3 cut(s) 72, 282, 423
HphI GGTGA 4 cut(s) 61, 121, 169, 181
Hpy166II GTNNAC 3 cut(s) 184, 466, 472
Hpy188I TCNGA 1 cut(s) 532
Hpy188III TCNNGA 3 cut(s) 85, 503, 552
Hpy8I GTNNAC 3 cut(s) 184, 466, 472
HpyAV CCTTC 2 cut(s) 115, 145
HpyCH4III ACNGT 3 cut(s) 181, 476, 487
HpyCH4IV ACGT 4 cut(s) 162, 378, 468, 507
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 356, 380, 428
HpySE526I ACGT 4 cut(s) 162, 378, 468, 507
Hsp92I GRCGYC 6 cut(s) 48, 162, 252, 378, 405, 507
Hsp92II CATG 1 cut(s) 235
HspAI GCGC 2 cut(s) 48, 252
KasI GGCGCC 2 cut(s) 47, 251
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
Kzo9I GATC 3 cut(s) 448, 490, 538
LmnI GCTCC 1 cut(s) 290
LpnPI CCDG 7 cut(s) 85, 295, 302, 356, 376, 436, 503
MaeII ACGT 4 cut(s) 162, 378, 468, 507
MalI GATC 3 cut(s) 450, 492, 540
MbiI CCGCTC 1 cut(s) 326
MboI GATC 3 cut(s) 448, 490, 538
MboII GAAGA 2 cut(s) 26, 232
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 2 cut(s) 210, 402
MluCI AATT 1 cut(s) 19
Mly113I GGCGCC 2 cut(s) 48, 252
MmeI TCCRAC 2 cut(s) 510, 516
MroNI GCCGGC 1 cut(s) 281
MspI CCGG 3 cut(s) 72, 282, 423
MvnI CGCG 2 cut(s) 86, 461
MwoI GCNNNNNNNGC 4 cut(s) 56, 356, 380, 428
NaeI GCCGGC 1 cut(s) 283
NarI GGCGCC 2 cut(s) 48, 252
NdeII GATC 3 cut(s) 448, 490, 538
NgoMIV GCCGGC 1 cut(s) 281
NlaIII CATG 1 cut(s) 235
NlaIV GGNNCC 5 cut(s) 49, 99, 253, 286, 427
NmeAIII GCCGAG 1 cut(s) 240
NruI TCGCGA 1 cut(s) 86
PcsI WCGNNNNNNNCGW 6 cut(s) 120, 168, 177, 375, 408, 465
PdiI GCCGGC 1 cut(s) 283
PflFI GACNNNGTC 1 cut(s) 113
PkrI GCNGC 5 cut(s) 8, 52, 361, 409, 430
PluTI GGCGCC 2 cut(s) 51, 255
PpuMI RGGWCCY 2 cut(s) 97, 310
Psp5II RGGWCCY 2 cut(s) 97, 310
PspN4I GGNNCC 5 cut(s) 49, 99, 253, 286, 427
PspPI GGNCC 3 cut(s) 97, 310, 463
PspPPI RGGWCCY 2 cut(s) 97, 310
PsuI RGATCY 1 cut(s) 538
PsyI GACNNNGTC 1 cut(s) 113
RruI TCGCGA 1 cut(s) 86
SalI GTCGAC 1 cut(s) 470
SatI GCNGC 5 cut(s) 7, 51, 360, 408, 429
Sau3AI GATC 3 cut(s) 448, 490, 538
Sau96I GGNCC 3 cut(s) 97, 310, 463
SduI GDGCHC 2 cut(s) 210, 402
SfoI GGCGCC 2 cut(s) 49, 253
SgrAI CRCCGGYG 1 cut(s) 422
SinI GGWCC 3 cut(s) 97, 310, 463
Sse9I AATT 1 cut(s) 19
SspDI GGCGCC 2 cut(s) 47, 251
StyI CCWWGG 3 cut(s) 93, 306, 432
TaaI ACNGT 3 cut(s) 181, 476, 487
TaiI ACGT 4 cut(s) 165, 381, 471, 510
TaqI TCGA 6 cut(s) 108, 123, 159, 402, 471, 504
TasI AATT 1 cut(s) 19
TauI GCSGC 5 cut(s) 9, 53, 362, 410, 431
TspGWI ACGGA 1 cut(s) 26
Tth111I GACNNNGTC 1 cut(s) 113
VpaK11BI GGWCC 3 cut(s) 97, 310, 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 471
ZraI GACGTC 3 cut(s) 163, 379, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.